Moc07g03880 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g03880
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	rRNA N-glycosidase
Location	chr7: 3381388 .. 3382244 (+)
RNA-Seq Expression	Moc07g03880
Synteny	Moc07g03880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAAATTGTTAGTGTTTTGTTTGCTAATTCTGGCCATCTTCCTTGCATCTCCTACGGTCGAGGGCGATGTGAACTTCAACTTGGCCACGGCTACTAAAACATCCTATGCAGCTTTCCTCAATGCCTTTAGAGCCGATCTTCCACTTGAAATTAAGAGACCCTTTGATATATCTCTACTGCGTGCCGCGGTTGCAGGCGCAGCTCGCTACACACGAATCCATCTTACCGACCGCAGTGGTCGTACCGTCACACTGGCTATAGATGTAACAAACGTTTACGTTCTTGGATTTTACTCCCCGCAGTGTAATCTATCCCACTTCTTTAATGACCCATTTCCATTGGAGCTATGGCATTCGTATTCACCAATTCTCAGCAAAGAAGACCTCCATTTGATGGCAGTTACCCACAACTTTCTGGGGCTGGAGCCAACCGAAGCTCTCTTGTCCTTGGCCTCCCATCCCTCAACAATTACATTCCAACGTTGGCTGGCTACAACTGCCCAAACACCAACTACCAGCCCTCCGACGTCGCGAAGGCGTGTGTGATAACCATTCAGACGATGTCGGAGGCAGCAAGATTTCAAAAGATTCAGGATGCAGTTCCAAACAATTTGGTTCCAAATCCAGAAATTCTATCTTTAGAAAACAATTGGGGTAGACTCTCCAGACAAGTTCAGCTAGCAGAGAGTAATGGTGGACGATTTACATCTAACGTCACGCTTCAAGACCCAACCGGTGCTACGGTTATGGTATCGAACGTTAATTCACCATATGTAAGAGGCAACATTCGGTTGCTGCTGAACCAACAGAATGCTCCAACTACCTCTGAACATGAAAATTATGCTACAATGTAA

mRNA sequence

ATGAGCAAATTGTTAGTGTTTTGTTTGCTAATTCTGGCCATCTTCCTTGCATCTCCTACGGTCGAGGGCGATGTGAACTTCAACTTGGCCACGGCTACTAAAACATCCTATGCAGCTTTCCTCAATGCCTTTAGAGCCGATCTTCCACTTGAAATTAAGAGACCCTTTGATATATCTCTACTGCGTGCCGCGGTTGCAGGCGCAGCTCGCTACACACGAATCCATCTTACCGACCGCAGTGGTCGTACCGTCACACTGGCTATAGATCAAAGAAGACCTCCATTTGATGGCAGTTACCCACAACTTTCTGGGGCTGGAGCCAACCGAAGCTCTCTTGTCCTTGGCCTCCCATCCCTCAACAATTACATTCCAACGTTGGCTGGCTACAACTGCCCAAACACCAACTACCAGCCCTCCGACGTCGCGAAGGCGTGTGTGATAACCATTCAGACGATGTCGGAGGCAGCAAGATTTCAAAAGATTCAGGATGCAGTTCCAAACAATTTGGTTCCAAATCCAGAAATTCTATCTTTAGAAAACAATTGGGGTAGACTCTCCAGACAAGTTCAGCTAGCAGAGAGTAATGGTGGACGATTTACATCTAACGTCACGCTTCAAGACCCAACCGGTGCTACGGTTATGGTATCGAACGTTAATTCACCATATGTAAGAGGCAACATTCGGTTGCTGCTGAACCAACAGAATGCTCCAACTACCTCTGAACATGAAAATTATGCTACAATGTAA

Coding sequence (CDS)

ATGAGCAAATTGTTAGTGTTTTGTTTGCTAATTCTGGCCATCTTCCTTGCATCTCCTACGGTCGAGGGCGATGTGAACTTCAACTTGGCCACGGCTACTAAAACATCCTATGCAGCTTTCCTCAATGCCTTTAGAGCCGATCTTCCACTTGAAATTAAGAGACCCTTTGATATATCTCTACTGCGTGCCGCGGTTGCAGGCGCAGCTCGCTACACACGAATCCATCTTACCGACCGCAGTGGTCGTACCGTCACACTGGCTATAGATCAAAGAAGACCTCCATTTGATGGCAGTTACCCACAACTTTCTGGGGCTGGAGCCAACCGAAGCTCTCTTGTCCTTGGCCTCCCATCCCTCAACAATTACATTCCAACGTTGGCTGGCTACAACTGCCCAAACACCAACTACCAGCCCTCCGACGTCGCGAAGGCGTGTGTGATAACCATTCAGACGATGTCGGAGGCAGCAAGATTTCAAAAGATTCAGGATGCAGTTCCAAACAATTTGGTTCCAAATCCAGAAATTCTATCTTTAGAAAACAATTGGGGTAGACTCTCCAGACAAGTTCAGCTAGCAGAGAGTAATGGTGGACGATTTACATCTAACGTCACGCTTCAAGACCCAACCGGTGCTACGGTTATGGTATCGAACGTTAATTCACCATATGTAAGAGGCAACATTCGGTTGCTGCTGAACCAACAGAATGCTCCAACTACCTCTGAACATGAAAATTATGCTACAATGTAA

Protein sequence

MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISLLRAAVAGAARYTRIHLTDRSGRTVTLAIDQRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM

Homology

[Close]

BLAST of Moc07g03880 vs. NCBI nr
Match: XP_022156706.1 (ribosome-inactivating protein bryodin I-like, partial [Momordica charantia])

HSP 1 Score: 413.3 bits (1061), Expect = 1.5e-111
Identity = 218/286 (76.22%), Postives = 232/286 (81.12%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M KLLVF LLIL IFLASPTVEGDV+FNL+TATKTSY AFLNAFRADLPLE K+P++ISL
Sbjct: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAID------------------------------- 120
           LRA VAGAARYTRIHLTDRSGR +TLAID                               
Sbjct: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120

Query: 121 -------QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVA 180
                  Q+  PFDGSYPQL+G GANRSSLVLGLPSLNN+IPTLAGYNCPNTNYQP+DVA
Sbjct: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA 180

Query: 181 KACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSN 240
           KACV+TIQTMSEAARFQKIQDAVPNNLVPN EILSLENNWG+LS+QVQLAESNGGRF SN
Sbjct: 181 KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN 240

Query: 241 VTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 249
           VTLQDPTGATV+VSNVNSPYVRGNIRLLLNQQN    SEHENYATM
Sbjct: 241 VTLQDPTGATVIVSNVNSPYVRGNIRLLLNQQNVQAMSEHENYATM 286

[Close]

BLAST of Moc07g03880 vs. NCBI nr
Match: XP_022159206.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 322.4 bits (825), Expect = 3.4e-84
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 90  QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKACVITI 149
           QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKACVITI
Sbjct: 10  QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKACVITI 69

Query: 150 QTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVTLQDPT 209
           QTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVTLQDPT
Sbjct: 70  QTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVTLQDPT 129

Query: 210 GATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 249
           GATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM
Sbjct: 130 GATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 168

[Close]

BLAST of Moc07g03880 vs. NCBI nr
Match: XP_022158190.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 275.8 bits (704), Expect = 3.7e-70
Identity = 159/286 (55.59%), Postives = 191/286 (66.78%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M+KLLVFCLLILA+FLA+PTVEGDV+FNL+  TKTSYA FLNA+RADLP + +  FDI L
Sbjct: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIIL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAID------------------------------- 120
           L   V G++RYTRIHLT+   +T+ LAID                               
Sbjct: 61  LPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKF 120

Query: 121 ------QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNN-YIPTLAGYNCPNTNYQPSDVA 180
                 Q+   FDGSY QL+GAG  R SL LGL  LN  YIPTL GYNCP  +Y+PSDVA
Sbjct: 121 VFTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVA 180

Query: 181 KACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSN 240
           KA V+ IQT+SEAARFQKIQ  V  N +P  E+LSLENNW +LS+ V+ A+   G F  N
Sbjct: 181 KAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVN 240

Query: 241 VTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 249
            TL++ TG  V+VSNVNSPYV+GNI+LLLN+QN   TS  ENYAT+
Sbjct: 241 FTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATS--ENYATI 283

[Close]

BLAST of Moc07g03880 vs. NCBI nr
Match: XP_022158194.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 256.5 bits (654), Expect = 2.3e-64
Identity = 146/271 (53.87%), Postives = 173/271 (63.84%), Query Frame = 0


Query: 3   KLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISLLR 62
           KL+VFCLLI+AIF A+P+VEGDV+F L   TK SY AFL  FR D+P+E K  F I LLR
Sbjct: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61

Query: 63  AAVAGAARYTRIHLTDRSGRTVTLAID--------------------------------- 122
           A V GA RYTRIHL+DR   T+TLAID                                 
Sbjct: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121

Query: 123 -----QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKA 182
                Q   PFDGSY +L  AG +R  L LGL ++N  IP L  Y+CPN+    SDVAKA
Sbjct: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKA 181

Query: 183 CVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVT 236
           CV+ IQT+SEAARFQ I++AV NN+ P  EILSLENNWG LS+QVQLA+ N GRF + V 
Sbjct: 182 CVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATVQ 241

[Close]

BLAST of Moc07g03880 vs. NCBI nr
Match: XP_022158438.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 180.3 bits (456), Expect = 2.1e-41
Identity = 125/298 (41.95%), Postives = 155/298 (52.01%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASP--TVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDI 60
           M KL VFCLL LAIFLA+P  TV+G     ++T    SY + LN FR  L  + K+  +I
Sbjct: 1   MRKLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIAL-RDNKKVCNI 60

Query: 61  SLLRAAVAGAARYTRIHLTDRSGRTVTLAID----------------------------- 120
            LLRA V    R+  I +   +  T+ LAID                             
Sbjct: 61  PLLRANVIDKNRFIEISIAS-TNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVR 120

Query: 121 -----------QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQP 180
                      Q+  PFDG YP     GANR SL LGL SLN+ +P L  Y C  T Y  
Sbjct: 121 ATQYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQ 180

Query: 181 SDVAKACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGR 240
           +DVAKA V+ +Q +SEAARF+ IQ AVPNN  PN E+LSL+N+W  LS  VQ A+   GR
Sbjct: 181 TDVAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGR 240

Query: 241 FTSNVTLQDPTGATVMVSNVNSPYVRGNIRLLL--NQQNAPTTS------EHENYATM 249
           F   VTL D  G  V V N++S YV GN++LLL  N   A   S       H+NYAT+
Sbjct: 241 FKDKVTLNDEKGHPVDVWNISSKYVSGNLKLLLFVNTTFAALPSVVDNNGGHQNYATI 296

[Close]

BLAST of Moc07g03880 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 157.1 bits (396), Expect = 2.5e-37
Identity = 103/282 (36.52%), Postives = 151/282 (53.55%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M KLLV  LLIL IFL SPTVEGDV+F L+ AT TSY  F+   R  LP E ++ ++I L
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYE-RKVYNIPL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAIDQRRP--------------------------- 120
           LR++++G+ RYT +HLT+ +  T+++A+D                               
Sbjct: 61  LRSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVF 120

Query: 121 ---------PFDGSYPQL-SGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAK 180
                    P+ G+Y +L + AG  R ++ LGLP+L++ I TL  Y         S  A 
Sbjct: 121 KDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTA-------SSAAS 180

Query: 181 ACVITIQTMSEAARF----QKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRF 240
           A ++ IQ+ +E+AR+    Q+I   V    +P+   +SLENNW  LS+Q+Q+A +N G+F
Sbjct: 181 ALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQF 240

Query: 241 TSNVTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSE 242
            S V L D     V ++N ++  V  NI LLLN+ N     E
Sbjct: 241 ESPVVLIDGNNQRVSITNASARVVTSNIALLLNRNNIAAIGE 274

[Close]

BLAST of Moc07g03880 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 151.8 bits (382), Expect = 1.0e-35
Identity = 101/276 (36.59%), Postives = 152/276 (55.07%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M + LVF LLIL +FL +P VEGDV+F L+ AT +SY  F++  R  LP E ++ +DI L
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYE-RKLYDIPL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAID------------------------------- 120
           LR+ + G+ RY  IHLT+ +  T+++AID                               
Sbjct: 61  LRSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVF 120

Query: 121 ---QRRP--PFDGSYPQLS-GAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAK 180
              +R+   P+ G+Y +L   AG  R ++ LGLP+L++ I TL  YN        +  A 
Sbjct: 121 KDAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNA-------NSAAS 180

Query: 181 ACVITIQTMSEAARF----QKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRF 236
           A ++ IQ+ SEAAR+    Q+I   V    +P+  I+SLEN+W  LS+Q+Q+A +N G+F
Sbjct: 181 ALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQF 240

[Close]

BLAST of Moc07g03880 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 2.3e-35
Identity = 100/276 (36.23%), Postives = 150/276 (54.35%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M + LV  LLIL +FL +P VEGDV+F L+ AT +SY  F++  R  LP E ++ +DI L
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNE-RKLYDIPL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAIDQRRP--------------------------- 120
           LR+++ G+ RY  IHLT+ +  T+++AID                               
Sbjct: 61  LRSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVF 120

Query: 121 ---------PFDGSYPQL-SGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAK 180
                    P+ G+Y +L + AG  R ++ LGLP+L++ I TL  YN        +  A 
Sbjct: 121 KDAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNA-------NSAAS 180

Query: 181 ACVITIQTMSEAARF----QKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRF 236
           A ++ IQ+ SEAAR+    Q+I   V    +P+  I+SLEN+W  LS+Q+Q+A +N G+F
Sbjct: 181 ALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQF 240

[Close]

BLAST of Moc07g03880 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-34
Identity = 105/286 (36.71%), Postives = 154/286 (53.85%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M K L+   LI+AIF+  PT +GDVNF+L+TAT  +Y  F+  FRA LP   K  +DI L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHK-VYDIPL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAID------------------QRRP--------- 120
           L + ++ + R+  ++LT  +  T+++AID                  +  P         
Sbjct: 61  LYSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFK 120

Query: 121 -------PFDGSYPQLSGAGAN-RSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKAC 180
                  P+ G+Y  L  A    R ++ LGLP+L++ I TL  YN  +    PS    A 
Sbjct: 121 GTRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSA---PS----AL 180

Query: 181 VITIQTMSEAARFQKIQ----DAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTS 240
           ++ IQT +EAARF+ I+      V  N  PN  I+SLEN W  LS+Q+ LA++ GG+F +
Sbjct: 181 LVLIQTTAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRN 240

Query: 241 NVTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYAT 248
            V L  PTG    V+NV+S  V+GNI+LLLN +    ++  EN+ T
Sbjct: 241 PVDLIKPTGERFQVTNVDSDVVKGNIKLLLNSR---ASTADENFIT 275

[Close]

BLAST of Moc07g03880 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.0e-34
Identity = 105/286 (36.71%), Postives = 153/286 (53.50%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M K L+   LI+AIF+  PT +GDVNF+L+TAT  +Y  F+  FRA LP   K  +DI L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHK-VYDIPL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAID------------------QRRP--------- 120
           L + ++ + R+  + LT  +  T+++AID                  +  P         
Sbjct: 61  LYSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFK 120

Query: 121 -------PFDGSYPQLSGAGAN-RSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKAC 180
                  P+ G+Y  L  A    R ++ LGLP+L++ I TL  YN  +    PS    A 
Sbjct: 121 GTRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSA---PS----AL 180

Query: 181 VITIQTMSEAARFQKIQ----DAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTS 240
           ++ IQT +EAARF+ I+      V  N  PN  I+SLEN W  LS+Q+ LA++ GG+F +
Sbjct: 181 LVLIQTTAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRN 240

Query: 241 NVTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYAT 248
            V L  PTG    V+NV+S  V+GNI+LLLN +    ++  EN+ T
Sbjct: 241 PVDLIKPTGERFQVTNVDSDVVKGNIKLLLNSR---ASTADENFIT 275

[Close]

BLAST of Moc07g03880 vs. ExPASy TrEMBL
Match: A0A6J1DVR9 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 7.1e-112
Identity = 218/286 (76.22%), Postives = 232/286 (81.12%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M KLLVF LLIL IFLASPTVEGDV+FNL+TATKTSY AFLNAFRADLPLE K+P++ISL
Sbjct: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAID------------------------------- 120
           LRA VAGAARYTRIHLTDRSGR +TLAID                               
Sbjct: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120

Query: 121 -------QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVA 180
                  Q+  PFDGSYPQL+G GANRSSLVLGLPSLNN+IPTLAGYNCPNTNYQP+DVA
Sbjct: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA 180

Query: 181 KACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSN 240
           KACV+TIQTMSEAARFQKIQDAVPNNLVPN EILSLENNWG+LS+QVQLAESNGGRF SN
Sbjct: 181 KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN 240

Query: 241 VTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 249
           VTLQDPTGATV+VSNVNSPYVRGNIRLLLNQQN    SEHENYATM
Sbjct: 241 VTLQDPTGATVIVSNVNSPYVRGNIRLLLNQQNVQAMSEHENYATM 286

[Close]

BLAST of Moc07g03880 vs. ExPASy TrEMBL
Match: A0A6J1E379 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111025625 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.7e-84
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 90  QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKACVITI 149
           QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKACVITI
Sbjct: 10  QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKACVITI 69

Query: 150 QTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVTLQDPT 209
           QTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVTLQDPT
Sbjct: 70  QTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVTLQDPT 129

Query: 210 GATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 249
           GATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM
Sbjct: 130 GATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 168

[Close]

BLAST of Moc07g03880 vs. ExPASy TrEMBL
Match: A0A6J1DV53 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.8e-70
Identity = 159/286 (55.59%), Postives = 191/286 (66.78%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISL 60
           M+KLLVFCLLILA+FLA+PTVEGDV+FNL+  TKTSYA FLNA+RADLP + +  FDI L
Sbjct: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIIL 60

Query: 61  LRAAVAGAARYTRIHLTDRSGRTVTLAID------------------------------- 120
           L   V G++RYTRIHLT+   +T+ LAID                               
Sbjct: 61  LPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKF 120

Query: 121 ------QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNN-YIPTLAGYNCPNTNYQPSDVA 180
                 Q+   FDGSY QL+GAG  R SL LGL  LN  YIPTL GYNCP  +Y+PSDVA
Sbjct: 121 VFTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVA 180

Query: 181 KACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSN 240
           KA V+ IQT+SEAARFQKIQ  V  N +P  E+LSLENNW +LS+ V+ A+   G F  N
Sbjct: 181 KAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVN 240

Query: 241 VTLQDPTGATVMVSNVNSPYVRGNIRLLLNQQNAPTTSEHENYATM 249
            TL++ TG  V+VSNVNSPYV+GNI+LLLN+QN   TS  ENYAT+
Sbjct: 241 FTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATS--ENYATI 283

[Close]

BLAST of Moc07g03880 vs. ExPASy TrEMBL
Match: A0A6J1DYP1 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 1.1e-64
Identity = 146/271 (53.87%), Postives = 173/271 (63.84%), Query Frame = 0


Query: 3   KLLVFCLLILAIFLASPTVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDISLLR 62
           KL+VFCLLI+AIF A+P+VEGDV+F L   TK SY AFL  FR D+P+E K  F I LLR
Sbjct: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61

Query: 63  AAVAGAARYTRIHLTDRSGRTVTLAID--------------------------------- 122
           A V GA RYTRIHL+DR   T+TLAID                                 
Sbjct: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121

Query: 123 -----QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSDVAKA 182
                Q   PFDGSY +L  AG +R  L LGL ++N  IP L  Y+CPN+    SDVAKA
Sbjct: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKA 181

Query: 183 CVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFTSNVT 236
           CV+ IQT+SEAARFQ I++AV NN+ P  EILSLENNWG LS+QVQLA+ N GRF + V 
Sbjct: 182 CVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATVQ 241

[Close]

BLAST of Moc07g03880 vs. ExPASy TrEMBL
Match: A0A6J1DW33 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.0e-41
Identity = 125/298 (41.95%), Postives = 155/298 (52.01%), Query Frame = 0


Query: 1   MSKLLVFCLLILAIFLASP--TVEGDVNFNLATATKTSYAAFLNAFRADLPLEIKRPFDI 60
           M KL VFCLL LAIFLA+P  TV+G     ++T    SY + LN FR  L  + K+  +I
Sbjct: 1   MRKLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIAL-RDNKKVCNI 60

Query: 61  SLLRAAVAGAARYTRIHLTDRSGRTVTLAID----------------------------- 120
            LLRA V    R+  I +   +  T+ LAID                             
Sbjct: 61  PLLRANVIDKNRFIEISIAS-TNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVR 120

Query: 121 -----------QRRPPFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQP 180
                      Q+  PFDG YP     GANR SL LGL SLN+ +P L  Y C  T Y  
Sbjct: 121 ATQYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQ 180

Query: 181 SDVAKACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGR 240
           +DVAKA V+ +Q +SEAARF+ IQ AVPNN  PN E+LSL+N+W  LS  VQ A+   GR
Sbjct: 181 TDVAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGR 240

Query: 241 FTSNVTLQDPTGATVMVSNVNSPYVRGNIRLLL--NQQNAPTTS------EHENYATM 249
           F   VTL D  G  V V N++S YV GN++LLL  N   A   S       H+NYAT+
Sbjct: 241 FKDKVTLNDEKGHPVDVWNISSKYVSGNLKLLLFVNTTFAALPSVVDNNGGHQNYATI 296

The following BLAST results are available for this feature:

BLAST of Moc07g03880 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156706.1	1.5e-111	76.22	ribosome-inactivating protein bryodin I-like, partial [Momordica charantia]	[more]
XP_022159206.1	3.4e-84	100.00	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]
XP_022158190.1	3.7e-70	55.59	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022158194.1	2.3e-64	53.87	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022158438.1	2.1e-41	41.95	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]

BLAST of Moc07g03880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33185	2.5e-37	36.52	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P24478	1.0e-35	36.59	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	2.3e-35	36.23	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P24817	1.2e-34	36.71	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	2.0e-34	36.71	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]

BLAST of Moc07g03880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVR9	7.1e-112	76.22	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1	[more]
A0A6J1E379	1.7e-84	100.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111025625 PE=3 SV=1	[more]
A0A6J1DV53	1.8e-70	55.59	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1	[more]
A0A6J1DYP1	1.1e-64	53.87	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1	[more]
A0A6J1DW33	1.0e-41	41.95	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1	[more]

BLAST of Moc07g03880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 158..237 e-value: 1.0E-22 score: 81.7
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 91..157 e-value: 9.3E-13 score: 50.3
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..90 e-value: 2.1E-8 score: 36.0
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 93..190 e-value: 1.5E-15 score: 57.4
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 90..236
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 90..236
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 149..165
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..236

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g03880.1	Moc07g03880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity