Moc07g03490 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g03490
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	rRNA N-glycosidase
Location	chr7: 3047411 .. 3048268 (-)
RNA-Seq Expression	Moc07g03490
Synteny	Moc07g03490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAATCCATAGCACTTTCTTTTTTCATTCTCACAATCTTTCTTGGCCCTCCTACTGCCAAGGGTGATGTTAGCTTCGATTTGTCCACAGCTACTAAAAACTCCTATACATCTTTTATCAGAGACTTGAGGAGTGCTCTTCCGACTAGAGGTACAGTGTGCAATATACCTGTGCTACCTTCGACTGCATCTGGGTTACAATGGTTCAGATTCTTCAGTCTCACCAATTATAACTACCAAACCATCACAGTGGCGGTAAATGTAACAAATGTTTATATCGCAGCGTATAGCACTGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCTTTCAGGCTCTTATTCGTAGGTACTCAAAAGGTAACACTTCCATATTCTGGTAATTACGATAGGCTTCAAAGTATTGTAGGCAAAAACCGAGATTTGATTGAGCTTGGAATCCCTACTCTAAGCAATGCGATTACAAACTTGTTTTACAACAACAACTACAAACTTACTGCAACCGCACTACTCGTACTCATCCAGTCGACTGCAGAAGCTGCAAGATTTAAATATATCGAGCAAGAAGTTTCTAAACATGTTAGCAGCAATTTTTTTCCAAATCAAGCAATCATAAGTTTGGAAAATAGTTGGGGTGCTCTCTCGAAACAAATCCAGATAGCAAATAGCAAAGGAACTGGACAATTTGACAAAGCTGTTGAGCTTATAACCATTGATGGCAAACGAGTTAGTGTAACCAATACTTCTACTGGAGTTGTAAAAAGCAATATCAAGCTCCTACTATACTACAAAGTCAATGGTGCTAATGACAATTATATCCCTACAATATTGCCCCTTGGAGCTATATGA

mRNA sequence

ATGATGAAATCCATAGCACTTTCTTTTTTCATTCTCACAATCTTTCTTGGCCCTCCTACTGCCAAGGGTGATGTTAGCTTCGATTTGTCCACAGCTACTAAAAACTCCTATACATCTTTTATCAGAGACTTGAGGAGTGCTCTTCCGACTAGAGGTACAGTGTGCAATATACCTGTGCTACCTTCGACTGCATCTGGGTTACAATGGTTCAGATTCTTCAGTCTCACCAATTATAACTACCAAACCATCACAGTGGCGGTAAATGTAACAAATGTTTATATCGCAGCGTATAGCACTGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCTTTCAGGCTCTTATTCGTAGGTACTCAAAAGGTAACACTTCCATATTCTGGTAATTACGATAGGCTTCAAAGTATTGTAGGCAAAAACCGAGATTTGATTGAGCTTGGAATCCCTACTCTAAGCAATGCGATTACAAACTTGTTTTACAACAACAACTACAAACTTACTGCAACCGCACTACTCGTACTCATCCAGTCGACTGCAGAAGCTGCAAGATTTAAATATATCGAGCAAGAAGTTTCTAAACATGTTAGCAGCAATTTTTTTCCAAATCAAGCAATCATAAGTTTGGAAAATAGTTGGGGTGCTCTCTCGAAACAAATCCAGATAGCAAATAGCAAAGGAACTGGACAATTTGACAAAGCTGTTGAGCTTATAACCATTGATGGCAAACGAGTTAGTGTAACCAATACTTCTACTGGAGTTGTAAAAAGCAATATCAAGCTCCTACTATACTACAAAGTCAATGGTGCTAATGACAATTATATCCCTACAATATTGCCCCTTGGAGCTATATGA

Coding sequence (CDS)

ATGATGAAATCCATAGCACTTTCTTTTTTCATTCTCACAATCTTTCTTGGCCCTCCTACTGCCAAGGGTGATGTTAGCTTCGATTTGTCCACAGCTACTAAAAACTCCTATACATCTTTTATCAGAGACTTGAGGAGTGCTCTTCCGACTAGAGGTACAGTGTGCAATATACCTGTGCTACCTTCGACTGCATCTGGGTTACAATGGTTCAGATTCTTCAGTCTCACCAATTATAACTACCAAACCATCACAGTGGCGGTAAATGTAACAAATGTTTATATCGCAGCGTATAGCACTGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCTTTCAGGCTCTTATTCGTAGGTACTCAAAAGGTAACACTTCCATATTCTGGTAATTACGATAGGCTTCAAAGTATTGTAGGCAAAAACCGAGATTTGATTGAGCTTGGAATCCCTACTCTAAGCAATGCGATTACAAACTTGTTTTACAACAACAACTACAAACTTACTGCAACCGCACTACTCGTACTCATCCAGTCGACTGCAGAAGCTGCAAGATTTAAATATATCGAGCAAGAAGTTTCTAAACATGTTAGCAGCAATTTTTTTCCAAATCAAGCAATCATAAGTTTGGAAAATAGTTGGGGTGCTCTCTCGAAACAAATCCAGATAGCAAATAGCAAAGGAACTGGACAATTTGACAAAGCTGTTGAGCTTATAACCATTGATGGCAAACGAGTTAGTGTAACCAATACTTCTACTGGAGTTGTAAAAAGCAATATCAAGCTCCTACTATACTACAAAGTCAATGGTGCTAATGACAATTATATCCCTACAATATTGCCCCTTGGAGCTATATGA

Protein sequence

MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVLPSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVGTQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQSTAEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELITIDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLGAI

Homology

[Close]

BLAST of Moc07g03490 vs. NCBI nr
Match: XP_022156709.1 (type I ribosome-inactivating protein trichoanguina-like [Momordica charantia])

HSP 1 Score: 550.4 bits (1417), Expect = 8.9e-153
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
           PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLGAI 286
           IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLGAI
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLGAI 285

[Close]

BLAST of Moc07g03490 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes anguina] >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 389.0 bits (998), Expect = 3.4e-104
Identity = 205/282 (72.70%), Postives = 232/282 (82.27%), Query Frame = 0


Query: 5   IALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVLPSTA 64
           +ALSFF L I LG PTA GDVSFDLSTATK SY+SFI  LR ALPT+GTVC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVGTQKV 124
           SG QWFRFF+LTNYN +T+TVAVNVTNVYI AY   AVSYFFEDT AEAF+L+F GT+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQSTAEAA 184
            LPYSGNYD+LQS+VGK RD+IELGIP LS+AITN+ Y  +Y+ TA ALLVLIQ TAEAA
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVY-YDYQSTAAALLVLIQCTAEAA 180

Query: 185 RFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELITIDGK 244
           R+KYIEQ+VS H+SSNF+PNQA+ISLEN WGALSKQIQIAN  G GQF+  VEL   DG 
Sbjct: 181 RYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGT 240

Query: 245 RVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIP-TILPLGAI 286
           R SVTNTS GVVK NIKLLLYYK +  ++  IP TIL  GA+
Sbjct: 241 RFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAM 281

[Close]

BLAST of Moc07g03490 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 377.9 bits (969), Expect = 7.9e-101
Identity = 197/281 (70.11%), Postives = 233/281 (82.92%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M K IA++ F++ I L  PT K D++FDLSTATK SY++FI  LR+ALPT  TVCNIP+L
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
           PSTASGL WF FF++TNYNYQTITVAVNVTNVYI AY   A SYFFEDT AEA +LLF  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           TQKVTLP++GNYD+LQSIVGK RDLIELGIP LS A+TN+ Y N  +LTA ALLVLIQS+
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNK-QLTAAALLVLIQSS 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIEQ+V++H+S  F+PNQA+ISLEN+WGALSKQIQIANS G GQF   VELI+
Sbjct: 181 AEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIS 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILP 282
            +G R SVT+TS GVV+SNIKLLLYYK++ A++N  PT +P
Sbjct: 241 PNGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPTRMP 280

[Close]

BLAST of Moc07g03490 vs. NCBI nr
Match: XP_023513430.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372.1 bits (954), Expect = 4.3e-99
Identity = 193/281 (68.68%), Postives = 229/281 (81.49%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M K IA+S F+L I L  PT K D++ DLSTATK SY++FI  +R+ALPT+ TVCNIP+L
Sbjct: 1   MNKLIAISSFLLAISLISPTLKADIALDLSTATKASYSAFITHIRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
           PST +GL  F FF++TNYNYQT+ VAVNVTNVYI AY  GA SYFFEDT A+A +LLF G
Sbjct: 61  PSTPTGLASFTFFTITNYNYQTVEVAVNVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAG 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           TQKVTLP++GNYD+LQSIVGK RD++ELGIP LS A+TN+ Y N  +LTA ALLVLIQST
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNK-QLTAAALLVLIQST 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIEQ+V++H+S  F+PNQA+ISLEN+WGALSKQIQIANS G GQF   VELI 
Sbjct: 181 AEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIG 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILP 282
             G R SVTNTS GVV+ NIKLLLYYK++ A+DN  PT +P
Sbjct: 241 PTGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSPTRMP 280

[Close]

BLAST of Moc07g03490 vs. NCBI nr
Match: KAG6592904.1 (Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025309.1 Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370.9 bits (951), Expect = 9.6e-99
Identity = 190/281 (67.62%), Postives = 233/281 (82.92%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M K IA++ F+L I L  PT K D++FDLSTATK SY++FI  LR+ALPT+ TVCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
           PSTA+GL WF FF++TNYNYQT+ VAVNV+NVYI AY   A SYFFEDT A+A +LLF G
Sbjct: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           TQKVTLP++GNYD+LQSIVGK RD++ELGIP LS A+TN+ Y N  + TA ALLVLIQST
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNK-QPTAAALLVLIQST 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIEQ+V++++S  F+PNQA+ISLEN+WGALSKQIQIANS G+GQF   VELI+
Sbjct: 181 AEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELIS 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILP 282
            +G R  VT+TS+GVV+ NIKLLLYYK++ A+DN  PT +P
Sbjct: 241 PNGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMP 280

[Close]

BLAST of Moc07g03490 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 389.0 bits (998), Expect = 4.5e-107
Identity = 205/282 (72.70%), Postives = 232/282 (82.27%), Query Frame = 0


Query: 5   IALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVLPSTA 64
           +ALSFF L I LG PTA GDVSFDLSTATK SY+SFI  LR ALPT+GTVC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVGTQKV 124
           SG QWFRFF+LTNYN +T+TVAVNVTNVYI AY   AVSYFFEDT AEAF+L+F GT+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQSTAEAA 184
            LPYSGNYD+LQS+VGK RD+IELGIP LS+AITN+ Y  +Y+ TA ALLVLIQ TAEAA
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVY-YDYQSTAAALLVLIQCTAEAA 180

Query: 185 RFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELITIDGK 244
           R+KYIEQ+VS H+SSNF+PNQA+ISLEN WGALSKQIQIAN  G GQF+  VEL   DG 
Sbjct: 181 RYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGT 240

Query: 245 RVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIP-TILPLGAI 286
           R SVTNTS GVVK NIKLLLYYK +  ++  IP TIL  GA+
Sbjct: 241 RFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAM 281

[Close]

BLAST of Moc07g03490 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 4.8e-77
Identity = 157/283 (55.48%), Postives = 209/283 (73.85%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M+K + LSF I+ IF+G PTAKGDV+FDLSTAT  +YT FI D R+ LP    V +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
            ST S  + F   +LT+Y Y+TI+VA++VTNVY+ AY T  VSYFF+++  EA+ +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           T+K+TLPY+GNY+ LQ+   K R+ I+LG+P LS+AIT LFY  N +   +ALLVLIQ+T
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFY-YNAQSAPSALLVLIQTT 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIE+ V+K+V++NF PN AIISLEN W ALSKQI +A ++G G+F   V+LI 
Sbjct: 181 AEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIK 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLG 284
             G+R  VTN  + VVK NIKLLL  + + A++N+I T+  LG
Sbjct: 241 PTGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTLLG 281

[Close]

BLAST of Moc07g03490 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 6.3e-77
Identity = 157/283 (55.48%), Postives = 208/283 (73.50%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M+K + LSF I+ IF+G PTAKGDV+FDLSTAT  +YT FI D R+ LP    V +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
            ST S  + F    LT+Y Y+TI+VA++VTNVY+ AY T  VSYFF+++  EA+ +LF G
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           T+K+TLPY+GNY+ LQ+   K R+ I+LG+P LS+AIT LFY  N +   +ALLVLIQ+T
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFY-YNAQSAPSALLVLIQTT 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIE+ V+K+V++NF PN AIISLEN W ALSKQI +A ++G G+F   V+LI 
Sbjct: 181 AEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIK 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLG 284
             G+R  VTN  + VVK NIKLLL  + + A++N+I T+  LG
Sbjct: 241 PTGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTLLG 281

[Close]

BLAST of Moc07g03490 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 253.4 bits (646), Expect = 2.9e-66
Identity = 149/285 (52.28%), Postives = 188/285 (65.96%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M++ + LS  ILT+FL  P  +GDVSF LS AT +SY  FI +LR ALP    + +IP+L
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSA-EAFRLLFV 120
            S+  G Q +    LTNY  +TI+VA++VTNVYI  Y  G  SYFF + SA EA + +F 
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 -GTQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQ 180
              +KVTLPYSGNY+RLQ+  GK R+ I LG+P L +AIT LFY  N    A+AL+VLIQ
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFY-YNANSAASALMVLIQ 180

Query: 181 STAEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVEL 240
           ST+EAAR+K+IEQ++ K V   F P+ AIISLENSW ALSKQIQIA S   GQF+  V L
Sbjct: 181 STSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFESPVVL 240

Query: 241 ITIDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLG 284
           I    +RV++TN   GVV SNI LLL      A D+ +P     G
Sbjct: 241 INAQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMTQSFG 283

[Close]

BLAST of Moc07g03490 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 250.4 bits (638), Expect = 2.5e-65
Identity = 148/285 (51.93%), Postives = 186/285 (65.26%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M++ +  S  ILT+FL  P  +GDVSF LS AT +SY  FI +LR ALP    + +IP+L
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSA-EAFRLLFV 120
            ST  G Q +    LTNY  +TI+VA++VTNVY+  Y  G  SYFF + SA EA + +F 
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 -GTQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQ 180
              +KVTLPYSGNY+RLQ   GK R+ I LG+P L +AIT LFY  N    A+AL+VLIQ
Sbjct: 121 DAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFY-YNANSAASALMVLIQ 180

Query: 181 STAEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVEL 240
           ST+EAAR+K+IEQ++ K V   F P+ AIISLENSW ALSKQIQIA S   GQF+  V L
Sbjct: 181 STSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFETPVVL 240

Query: 241 ITIDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLG 284
           I    +RV++TN   GVV SNI LLL      A D+ +P     G
Sbjct: 241 INAQNQRVTITNVDAGVVTSNIALLLNRNNMAAIDDDVPMAQSFG 283

[Close]

BLAST of Moc07g03490 vs. ExPASy TrEMBL
Match: A0A6J1DUC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.3e-153
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
           PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLGAI 286
           IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLGAI
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLGAI 285

[Close]

BLAST of Moc07g03490 vs. ExPASy TrEMBL
Match: A0A6J1KWC4 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.8e-101
Identity = 197/281 (70.11%), Postives = 233/281 (82.92%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M K IA++ F++ I L  PT K D++FDLSTATK SY++FI  LR+ALPT  TVCNIP+L
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
           PSTASGL WF FF++TNYNYQTITVAVNVTNVYI AY   A SYFFEDT AEA +LLF  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           TQKVTLP++GNYD+LQSIVGK RDLIELGIP LS A+TN+ Y N  +LTA ALLVLIQS+
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNK-QLTAAALLVLIQSS 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIEQ+V++H+S  F+PNQA+ISLEN+WGALSKQIQIANS G GQF   VELI+
Sbjct: 181 AEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIS 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILP 282
            +G R SVT+TS GVV+SNIKLLLYYK++ A++N  PT +P
Sbjct: 241 PNGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPTRMP 280

[Close]

BLAST of Moc07g03490 vs. ExPASy TrEMBL
Match: A0A6J1H951 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 4.8e-96
Identity = 186/281 (66.19%), Postives = 230/281 (81.85%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           M K IA++ F+L I L  PT K D++ DLSTATK +Y++FI  LR+ALPT+ TVCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
           PSTA+GL  F FF++TNYNY+T+ VAVNV+NVYI AY   A SYFFEDTSA+A +LLF  
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           TQK+TLP++GNYDRLQ +VGK RD IELGIP LS A+TN+ Y N  +LTA ALLVLIQST
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNK-QLTAAALLVLIQST 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIEQ+V++++S  F+PNQA+ISLEN+WGALSKQIQIANS G+GQF   VELI+
Sbjct: 181 AEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELIS 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILP 282
            +G R  VT+TS+GVV+ NIKLLLYYK++ A+DN  PT +P
Sbjct: 241 PNGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMP 280

[Close]

BLAST of Moc07g03490 vs. ExPASy TrEMBL
Match: A0A6J1DR40 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 8.6e-85
Identity = 176/271 (64.94%), Postives = 206/271 (76.01%), Query Frame = 0


Query: 2   MKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVLP 61
           M+ +     +L I LGP +A+GDVSF LS A K SYT+FI+ LR A+PT  TVC IPVLP
Sbjct: 1   MRKLTTLSLLLAILLGPLSAEGDVSFQLSGANKKSYTNFIKQLRDAVPTTTTVCKIPVLP 60

Query: 62  STASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVGT 121
           STASG + F F  LTNY  QT+ VA+N+TNVYI AY    VSYFF+D S EA+++LF GT
Sbjct: 61  STASGPKSFTFVHLTNYADQTVVVAINITNVYIVAYHIDHVSYFFDDASPEAYKVLFEGT 120

Query: 122 QKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQSTA 181
            + TLPYS NYD+LQSIVGK RDLIELG+P LS+AITNL Y   YK TA ALLVLIQS A
Sbjct: 121 DRKTLPYSSNYDKLQSIVGKKRDLIELGLPALSSAITNLVY-YEYKSTAAALLVLIQSVA 180

Query: 182 EAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELITI 241
           EAAR+KYIEQE+S HV   F P+QAIISLEN+WGALSKQIQIA +KG GQF+K VELI  
Sbjct: 181 EAARYKYIEQEISTHVDDKFKPDQAIISLENNWGALSKQIQIA-AKGNGQFEKPVELINP 240

Query: 242 DGKRVSVTNTSTGVVKSNIKLLLYYKVNGAN 273
           +    SVTN S+ VV SNIKLLLY+K+  AN
Sbjct: 241 NNTPFSVTNVSSLVVTSNIKLLLYFKLAAAN 269

[Close]

BLAST of Moc07g03490 vs. ExPASy TrEMBL
Match: A0A6J1DRC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 6.1e-75
Identity = 158/283 (55.83%), Postives = 209/283 (73.85%), Query Frame = 0


Query: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60
           MMK + LSF I+ IF+G PTAKGDV+FDLSTAT  +YT FI D R+ LP    V +IP+L
Sbjct: 1   MMKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120
            ST S  + F   +LT+Y Y+TI+VA++VTNVY+ AY T  VSYFF+++  EA+ +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180
           T+K+TLPY+GNY+ LQ+   K R+ I+LG+P LS+AIT LFY  N +   +ALLVLIQ+T
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFY-YNAQSAPSALLVLIQTT 180

Query: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240
           AEAARFKYIE+ V+K+V++NF PN AIISLEN W ALSKQI +A ++G G+F   V+LI 
Sbjct: 181 AEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIK 240

Query: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILPLG 284
             G+R  VTN  + VVK NIKLLL  + + A++N+I T+  LG
Sbjct: 241 PTGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTLLG 281

The following BLAST results are available for this feature:

BLAST of Moc07g03490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156709.1	8.9e-153	100.00	type I ribosome-inactivating protein trichoanguina-like [Momordica charantia]	[more]
P56626.2	3.4e-104	72.70	RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt...	[more]
XP_023005055.1	7.9e-101	70.11	type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima]	[more]
XP_023513430.1	4.3e-99	68.68	type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. p...	[more]
KAG6592904.1	9.6e-99	67.62	Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrospe...	[more]

BLAST of Moc07g03490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56626	4.5e-107	72.70	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P24817	4.8e-77	55.48	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	6.3e-77	55.48	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P09989	2.9e-66	52.28	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P24478	2.5e-65	51.93	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]

BLAST of Moc07g03490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUC7	4.3e-153	100.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1	[more]
A0A6J1KWC4	3.8e-101	70.11	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1	[more]
A0A6J1H951	4.8e-96	66.19	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1	[more]
A0A6J1DR40	8.6e-85	64.94	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1	[more]
A0A6J1DRC7	6.1e-75	55.83	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1	[more]

BLAST of Moc07g03490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 143..157 score: 47.02 coord: 205..222 score: 51.02 coord: 173..193 score: 65.54 coord: 35..48 score: 42.86 coord: 82..97 score: 48.68
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..184 e-value: 5.7E-43 score: 148.4
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..222 e-value: 4.3E-33 score: 114.6
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 24..272
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 185..270 e-value: 6.8E-27 score: 95.1
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 24..272
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 177..193
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..269

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g03490.1	Moc07g03490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity