Moc07g03210 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g03210
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	rRNA N-glycosidase
Location	chr7: 2874991 .. 2877622 (+)
RNA-Seq Expression	Moc07g03210
Synteny	Moc07g03210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAAATTCTCAGCATTTCTTTCCATAATTCTTGCCATCTTCGTCCTTGATGTTCCTTCTATCAAGGGCCGTGATCCTCTCAACTTCAATTTGCTAGGCGTTTCTTCAAAATCCTACAAAAACTTCATCGAAAAGCAACTGAGGCCAGCTCTTCCATCCAATGAACAAGTGTACAGCATACCAGTGTTGCTCCCCACCATAGCTGACAAAGACCGCTTCCTACTCATCAATCTCGCCAACTACGACAGCGAAACCATCACGGTGGCTGTAGACGTATTGAACGTGTACGTCGTGGCATATCGTAGCGGCGACGTATCCTACTAAACAACGAGACGAATCTCCGCACCGCTTTTAAAGTACTATTCAAAGACAGTCCCCGCCATGTAGACCTCGATTATTCCAGCAACTACAAGGATCTTGAAGGCGCTGCACATGCATCAAAAGAGGAGATTCCTCTTGGATTTCCTGCCCTTGACGCTGCCATTACAAACATGATATCTTATAACAATTATAGAGCTGTGGCGAAGGCGTTTATCGTAATTATCCAGACATTCGCCGAGGCTGCAAGATTTCAGTCTATTATGGCAGAAATTGCTGGTATTGCTGCTGATGGTTCGGCTAAGCCAAGTGCATCAATGTTGAGTTTGGAAACTCAATGGTCTGCTCTCTCCAAAAATATTCAGTATGCAAAGACTAATGATGGACAGTTTCAAACACCTGTTGAGATTATAGACGGTAAAAATAACAAAGTCAAAATAACCAATGTTACCTCGAAAGTTGTGACTTCCAACGTCAAATTGCTGCTGAACAATCGAGATAAAGCCATGGTGATGCCATCCAATTGTGGCTACACAATGGGAAGAAAGTGGATGGTTGATGATGGATGTGAAAATCATGGATGGTTGATAATGTAAGCTTTTAAGTTTTAGTGTCTCTCAGTTTTCTGCTTTGCATCAAGAATATGTTGTCAAAATAAGGCTGGTCCATGTAAGTGTTAAATCTACTACAACAAATCCTGTCTATAGGACAAGGTTTTGTGTAGAGTTTTTAGTACTTCATAAGATATAATTATAAACCCTAAAGTTGTCTAGTATTTATTTAATTCATTTAGCTTTTATTATGTACTAGTTCTACAGCCCCATTTTGTCACGTTCATGTTTTTTGGTTTCTACTTCTTAATTTTTATACAGAAAAATGAATTGCATGGTCCCAAGATTCATGGATTCAAGTATGATAAAACAATCTTTTTATTTATGTTATGGAACAACTTGGAAATGATAACAAGAAAGAAGAAACTTGTTTTGGTTGTTTTTAAAATTTCTATACAATTTTTAGAATGTTCAAAAAAAAAAAAGAAGAAGAAAAACAAGAAAAGAGAAACAAAAACATAGGTAGTTACAAAATGCTTAAATTTCTTAAGAAATTTGGAAACGAGAAACCATAACGTTACATTGGGATTGTAAGAGAAATGCAAACAAAGTCTCAGATTGACTAGACAAGAGAATGATCTACGGCATATAGATGAGGACAACTAGCTCCACTGGTATGAGACCTAACCGTGAGAGTTATGCCCAAACGGGCCCTACGTGTAATGTACCTGCTGATATTTCAACTTTACCCTTATTCTACCTTTAATGATATTTGCACTTGAATTTCCATCTCTATCAAATAAGGCTTAGTTTAGTTTTAATATTTTCATTTGCTTCTTGGATATTGATTTTTGATTACTGTAACAATTTTTTACCAATGTGTATATATGCATAATGCCTCGATGATATTTGCACTTACATTTTTCATCTCTATAAAAATAATATGTTGTTAGTTTTATATTCATATTGGCTTTTTTGTATATTTACTGTTATTATTTGTACAAATTTTCACCAAATATTGTTGAGGGTAAAATCAAGCAGGACGCGTGTCCACTTCCATGAGGTGGGGACCGCAGACGGTTACACACCGTACGTTACAATGACAGGGGATACGTGTTTCCATCTCCCAAGTACGGATAGCACATCGAGCTCTAGCGGCAGGCCACAGGCTCCAGGCCTGCAGGCGGTGGCGCCACCTCTTGTTGTCGGGGACATACACCCTCAGCAAACGTCGTACCTCGCAGCTTGTCCCTACCATCCCTACCGTCGTCCTTCCAAGTCGGCTGCTCAGGCCTATATAAGGAAGGACCATCACCCTTACCAAGTAAGTGAAAACCTGTCATTAATACTTAAGATGTCTTTTTCCCTATTATCTACTCATGTTATTAACTTGAGCATCAGAGCGTATTAGGTCGGACACCGCACCGGTTTCTAGTTTTCTAGTTGCAAGCTCATTTGTTCTTAGGTGAAGCTACTCGTTCGAGGGACATGTGGACCTCTCCAAGATTCGCCATCAACAGTTGGTGCCGTCTGTGGGAACGACATTTTCAAGCTGAAACAAAAACTATTTTTTTTGGTACTAAGACATCACTAAAATGGCTTTCGATAAGGAGTCATCTTATGAAAACTCTGTCGACGAAGAACGTCGAGGCGATAAGCTAGTTGACAAGCCTGCTCCGACTCTTCCTGCACTGCAGAAGGCTAGAGAAGCACGGGACGCTGTCAACCCCCTTCGCCTCATCGGCCACCTCAGAAAGTAA

mRNA sequence

ATGAACAAATTCTCAGCATTTCTTTCCATAATTCTTGCCATCTTCGTCCTTGATGTTCCTTCTATCAAGGGCCGTGATCCTCTCAACTTCAATTTGCTAGGCGTTTCTTCAAAATCCTACAAAAACTTCATCGAAAAGCAACTGAGGCCAGCTCTTCCATCCAATGAACAAGTGTACAGCATACCAGTGTTGCTCCCCACCATAGCTGACAAAGACCGCTTCCTACTCATCAATCTCGCCAACTACGACAGCGAAACCATCACGGTGGCTGTAGACGTATTGAACGTTCCCCGCCATGTAGACCTCGATTATTCCAGCAACTACAAGGATCTTGAAGGCGCTGCACATGCATCAAAAGAGGAGATTCCTCTTGGATTTCCTGCCCTTGACGCTGCCATTACAAACATGATATCTTATAACAATTATAGAGCTGTGGCGAAGGCGTTTATCGTAATTATCCAGACATTCGCCGAGGCTGCAAGATTTCAGTCTATTATGGCAGAAATTGCTGGTATTGCTGCTGATGGTTCGGCTAAGCCAAGTGCATCAATGTTGAGTTTGGAAACTCAATGGTCTGCTCTCTCCAAAAATATTCAGTATGCAAAGACTAATGATGGACAGTTTCAAACACCTGTTGAGATTATAGACGGGGATACGTGTTTCCATCTCCCAAGTACGGATAGCACATCGAGCTCTAGCGGCAGGCCACAGGCTCCAGGCCTGCAGGCGGTGGCGCCACCTCTTGTTGTCGGGGACATACACCCTCAGCAAACGTCGTACCTCGCAGCTTGTCCCTACCATCCCTACCGTCGTCCTTCCAAGTCGGCTGCTCAGGCCTATATAAGGAAGGACCATCACCCTTACCAAGAGTCATCTTATGAAAACTCTGTCGACGAAGAACGTCGAGGCGATAAGCTAGTTGACAAGCCTGCTCCGACTCTTCCTGCACTGCAGAAGGCTAGAGAAGCACGGGACGCTGTCAACCCCCTTCGCCTCATCGGCCACCTCAGAAAGTAA

Coding sequence (CDS)

ATGAACAAATTCTCAGCATTTCTTTCCATAATTCTTGCCATCTTCGTCCTTGATGTTCCTTCTATCAAGGGCCGTGATCCTCTCAACTTCAATTTGCTAGGCGTTTCTTCAAAATCCTACAAAAACTTCATCGAAAAGCAACTGAGGCCAGCTCTTCCATCCAATGAACAAGTGTACAGCATACCAGTGTTGCTCCCCACCATAGCTGACAAAGACCGCTTCCTACTCATCAATCTCGCCAACTACGACAGCGAAACCATCACGGTGGCTGTAGACGTATTGAACGTTCCCCGCCATGTAGACCTCGATTATTCCAGCAACTACAAGGATCTTGAAGGCGCTGCACATGCATCAAAAGAGGAGATTCCTCTTGGATTTCCTGCCCTTGACGCTGCCATTACAAACATGATATCTTATAACAATTATAGAGCTGTGGCGAAGGCGTTTATCGTAATTATCCAGACATTCGCCGAGGCTGCAAGATTTCAGTCTATTATGGCAGAAATTGCTGGTATTGCTGCTGATGGTTCGGCTAAGCCAAGTGCATCAATGTTGAGTTTGGAAACTCAATGGTCTGCTCTCTCCAAAAATATTCAGTATGCAAAGACTAATGATGGACAGTTTCAAACACCTGTTGAGATTATAGACGGGGATACGTGTTTCCATCTCCCAAGTACGGATAGCACATCGAGCTCTAGCGGCAGGCCACAGGCTCCAGGCCTGCAGGCGGTGGCGCCACCTCTTGTTGTCGGGGACATACACCCTCAGCAAACGTCGTACCTCGCAGCTTGTCCCTACCATCCCTACCGTCGTCCTTCCAAGTCGGCTGCTCAGGCCTATATAAGGAAGGACCATCACCCTTACCAAGAGTCATCTTATGAAAACTCTGTCGACGAAGAACGTCGAGGCGATAAGCTAGTTGACAAGCCTGCTCCGACTCTTCCTGCACTGCAGAAGGCTAGAGAAGCACGGGACGCTGTCAACCCCCTTCGCCTCATCGGCCACCTCAGAAAGTAA

Protein sequence

MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYSIPVLLPTIADKDRFLLINLANYDSETITVAVDVLNVPRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIIDGDTCFHLPSTDSTSSSSGRPQAPGLQAVAPPLVVGDIHPQQTSYLAACPYHPYRRPSKSAAQAYIRKDHHPYQESSYENSVDEERRGDKLVDKPAPTLPALQKAREARDAVNPLRLIGHLRK

Homology

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BLAST of Moc07g03210 vs. NCBI nr
Match: XP_022150386.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 416.0 bits (1068), Expect = 3.1e-112
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS
Sbjct: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNVPRHVDLDYSSNYKDLEGAAHASKE 120
           IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNVPRHVDLDYSSNYKDLEGAAHASKE
Sbjct: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNVPRHVDLDYSSNYKDLEGAAHASKE 120

Query: 121 EIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAKP 180
           EIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAKP
Sbjct: 121 EIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAKP 180

Query: 181 SASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIIDG 218
           SASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIIDG
Sbjct: 181 SASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIIDG 217

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BLAST of Moc07g03210 vs. NCBI nr
Match: XP_038884329.1 (putative ribosome-inactivating protein [Benincasa hispida])

HSP 1 Score: 164.1 bits (414), Expect = 2.1e-36
Identity = 103/246 (41.87%), Postives = 151/246 (61.38%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MN+FS    IIL IFV  VP+ +G + + FN+LG +SKSY+NFI   LR ALP+   VY+
Sbjct: 1   MNRFSVVCLIILNIFVHGVPTAEGDNDVIFNMLGSNSKSYRNFI-TSLRKALPNAGDVYN 60

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           IP+L+P++    R++++ L+NY+ ++I VAVDV NV                        
Sbjct: 61  IPLLVPSLPGPRRYIVMQLSNYEEKSIKVAVDVTNVYIMGYLVNSTSYFFDETDAQTASK 120

Query: 121 -----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIV 180
                 + + L YSSNY+ L+ AA   ++ IPLGF ALD+AI+ +  Y + +A   AF+V
Sbjct: 121 YVFQNTKMITLPYSSNYQKLQNAARKDRDSIPLGFKALDSAISTLY-YYDAKAAPAAFLV 180

Query: 181 IIQTFAEAARFQSI-MAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQT 217
           +IQ+ AEA+RF+ I    I GI+   +  PS + +SLE +WS LSK IQ AK+N+G FQ 
Sbjct: 181 LIQSTAEASRFKYIEQLIIDGISE--NKLPSLAAISLENEWSLLSKQIQLAKSNNGLFQI 240

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BLAST of Moc07g03210 vs. NCBI nr
Match: XP_023005049.1 (ribosome-inactivating protein cucurmosin [Cucurbita maxima])

HSP 1 Score: 162.9 bits (411), Expect = 4.7e-36
Identity = 107/244 (43.85%), Postives = 140/244 (57.38%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MNK  A   + LAIF+   P+      + F+L G +S SYK FI K LR ALP + +VY 
Sbjct: 11  MNKSLALSFLFLAIFLNTRPADAN---VRFDLSGATSSSYKTFI-KNLREALPKDGKVYD 70

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           I VLL T+ D  RF+LI+L NYD ++IT A+DVLNV                        
Sbjct: 71  ISVLLSTVMDSKRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQIPAQAPKL 130

Query: 121 ----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIVI 180
                +   L Y+ NY++L+ AA   +E I LG PALD+AIT +  Y N  A A A +V+
Sbjct: 131 LFKGTQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHY-NAEAAASALLVL 190

Query: 181 IQTFAEAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQTPV 217
           IQT +EAARF+ I  +IA        KPS +++SLE  WSALSK IQ AK  +GQF+TPV
Sbjct: 191 IQTTSEAARFRYIELQIANNVGT-KFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPV 248

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BLAST of Moc07g03210 vs. NCBI nr
Match: XP_022992350.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima])

HSP 1 Score: 162.2 bits (409), Expect = 8.1e-36
Identity = 108/246 (43.90%), Postives = 143/246 (58.13%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MN  S F  +ILAIF      + G   ++FNLLG +SKSY NFI K LR ALPS ++VY+
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAG--DVSFNLLGSNSKSYSNFI-KALRSALPSKDKVYN 70

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           I +LL +     R+  + L+NYD + ITVA+DV NV                        
Sbjct: 71  ISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASK 130

Query: 121 -----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIV 180
                   + L YS NY+ L+ AA   +E+IPLGFPALD+AIT +  Y++  A A AFIV
Sbjct: 131 YVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDS-TAAAAAFIV 190

Query: 181 IIQTFAEAARFQSIMAE-IAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQT 217
           +IQ  AEA+R++ I A+ I  I+ D    PS +++SLE  WSALSK IQ A+TN G F+ 
Sbjct: 191 VIQCTAEASRYKYIEAQMIKRISKDD--VPSPAIISLENSWSALSKQIQLAQTNKGVFKA 250

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BLAST of Moc07g03210 vs. NCBI nr
Match: XP_022953306.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata])

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-35
Identity = 108/246 (43.90%), Postives = 142/246 (57.72%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MN  S F  +ILAIF      + G   ++FNLLG +SKSY NFIE  LR ALPS ++VY+
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAG--DVSFNLLGSNSKSYSNFIE-ALRSALPSKDKVYN 70

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           I +LL +     R+  + L+NYD + ITVA+DV NV                        
Sbjct: 71  ISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASK 130

Query: 121 -----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIV 180
                   + L YS NY+ L+ AA   +E+IPLGFPALD AIT +  Y++  A A AFIV
Sbjct: 131 YVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDS-TAAAAAFIV 190

Query: 181 IIQTFAEAARFQSIMAE-IAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQT 217
           +IQ  AEA+R++ I A+ I  I+ D    PS +++SLE  WSALSK IQ A+TN G F+ 
Sbjct: 191 VIQCTAEASRYRYIEAQMIKRISKDD--VPSLAIISLENSWSALSKQIQLAQTNKGVFKA 250

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BLAST of Moc07g03210 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.3e-38
Identity = 96/245 (39.18%), Postives = 144/245 (58.78%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MN+FS  + +++      VP+ +G   + F+LLG + KSY  FI   +R ALP+   +Y+
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFI-TSMRNALPNAGDIYN 60

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           IP+L+P+I+   R++L+ L+NY+  TIT+AVDV NV                        
Sbjct: 61  IPLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASK 120

Query: 121 -----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIV 180
                 + + L YS NY+ L+  A   ++ IPLGF ALD+AI+ +  Y++ R+   AF+V
Sbjct: 121 FVFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDS-RSAPIAFLV 180

Query: 181 IIQTFAEAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQTP 217
           +IQT AEAAR++ I  +I       S  P  + +SLE +WS LSK IQ AK+N+GQFQTP
Sbjct: 181 LIQTTAEAARYKYIEKQIID-RISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTP 240

[Close]

BLAST of Moc07g03210 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 157.9 bits (398), Expect = 2.0e-37
Identity = 98/217 (45.16%), Postives = 128/217 (58.99%), Query Frame = 0


Query: 28  LNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYSIPVLLPTIADKDRFLLINLANYDSETI 87
           + F+L   +S SYK FI K LR ALP + +VY IPVLL T+ D  RF+LI+L NYD ++I
Sbjct: 2   VRFDLSSATSSSYKTFI-KNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 61

Query: 88  TVAVDVLNV----------------------------PRHVDLDYSSNYKDLEGAAHASK 147
           T A+DVLNV                             +   L Y+ NY++L+ AA   +
Sbjct: 62  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 121

Query: 148 EEIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAK 207
           E I LG PALD+AIT +  Y N  A A A +V+IQT +EAARF+ I  +IA        K
Sbjct: 122 ENIELGLPALDSAITTLFHY-NAEAAASALLVLIQTTSEAARFRYIELQIANNVGT-KFK 181

Query: 208 PSASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIID 217
           PS +++SLE  WSALSK IQ AK  +GQF+TPV +ID
Sbjct: 182 PSQTIISLENNWSALSKQIQIAKNKNGQFETPVILID 215

[Close]

BLAST of Moc07g03210 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.3e-36
Identity = 104/246 (42.28%), Postives = 145/246 (58.94%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           M++FS    +ILAIF L    +KG   ++F L G   +SY  FI K LR ALP  E+VY+
Sbjct: 1   MSRFSVLSFLILAIF-LGGSIVKG--DVSFRLSGADPRSYGMFI-KDLRNALPFREKVYN 60

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           IP+LLP+++   R+LL++L NYD +TITVAVDV NV                        
Sbjct: 61  IPLLLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQ 120

Query: 121 ------PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFI 180
                  R + L YS NY+ L+ AA   +E+IP+G PALD+AI+ ++ Y++  A A A +
Sbjct: 121 YVFRDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDS-TAAAGALL 180

Query: 181 VIIQTFAEAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQT 217
           V+IQT AEAARF+ I  +I   A      PS + +SLE  WS LSK IQ A+ N+G F+T
Sbjct: 181 VLIQTTAEAARFKYIEQQIQERAYRDEV-PSLATISLENSWSGLSKQIQLAQGNNGIFRT 240

[Close]

BLAST of Moc07g03210 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 152.5 bits (384), Expect = 8.4e-36
Identity = 102/246 (41.46%), Postives = 140/246 (56.91%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           M +F  F  +IL +F L  P+++G   ++F L G +S SY  FI   LR ALP   ++Y 
Sbjct: 1   MIRFLVFSLLILTLF-LTAPAVEG--DVSFRLSGATSSSYGVFI-SNLRKALPYERKLYD 60

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           IP+L  T+    R+ LI+L NY  ETI+VA+DV NV                        
Sbjct: 61  IPLLRSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAK 120

Query: 121 ------PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFI 180
                  R V L YS NY+ L+ AA   +E IPLG PALD+AIT +  Y N  + A A +
Sbjct: 121 YVFKDAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLF-YYNANSAASALM 180

Query: 181 VIIQTFAEAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQT 217
           V+IQ+ +EAAR++ I  +I G   D +  PS +++SLE  WSALSK IQ A TN+GQF+T
Sbjct: 181 VLIQSTSEAARYKFIEQQI-GKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFET 240

[Close]

BLAST of Moc07g03210 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-35
Identity = 107/239 (44.77%), Postives = 137/239 (57.32%), Query Frame = 0


Query: 7   FLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYSIPVLLP 66
           F  +ILAIFV    S    D + F+L G SS SY  FI   LR ALPSN  VY+I +LL 
Sbjct: 4   FTVLILAIFV--AASTVEAD-VRFSLSGSSSTSYSKFI-GDLRKALPSNGTVYNITLLLS 63

Query: 67  TIADKDRFLLINLANYDSETITVAVDVLNV-----------------------------P 126
           + +   R+ L+ L+NYD + ITVAVDV NV                              
Sbjct: 64  SASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGS 123

Query: 127 RHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFA 186
             V L YS NY+ L+ AA   +E+IPLGFPALD+AIT +  Y++  A A AF+VIIQT A
Sbjct: 124 TIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDS-TAAAAAFLVIIQTTA 183

Query: 187 EAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIID 217
           EA+RF+ I  +I    +     PS + +SLE +WSALSK IQ A+TN+G F+TPV I D
Sbjct: 184 EASRFKYIEGQIIERISKNQV-PSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITD 236

[Close]

BLAST of Moc07g03210 vs. ExPASy TrEMBL
Match: A0A6J1DBC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111018562 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.5e-112
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS
Sbjct: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNVPRHVDLDYSSNYKDLEGAAHASKE 120
           IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNVPRHVDLDYSSNYKDLEGAAHASKE
Sbjct: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNVPRHVDLDYSSNYKDLEGAAHASKE 120

Query: 121 EIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAKP 180
           EIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAKP
Sbjct: 121 EIPLGFPALDAAITNMISYNNYRAVAKAFIVIIQTFAEAARFQSIMAEIAGIAADGSAKP 180

Query: 181 SASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIIDG 218
           SASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIIDG
Sbjct: 181 SASMLSLETQWSALSKNIQYAKTNDGQFQTPVEIIDG 217

[Close]

BLAST of Moc07g03210 vs. ExPASy TrEMBL
Match: A0A6J1KY21 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498157 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 2.3e-36
Identity = 107/244 (43.85%), Postives = 140/244 (57.38%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MNK  A   + LAIF+   P+      + F+L G +S SYK FI K LR ALP + +VY 
Sbjct: 11  MNKSLALSFLFLAIFLNTRPADAN---VRFDLSGATSSSYKTFI-KNLREALPKDGKVYD 70

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           I VLL T+ D  RF+LI+L NYD ++IT A+DVLNV                        
Sbjct: 71  ISVLLSTVMDSKRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQIPAQAPKL 130

Query: 121 ----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIVI 180
                +   L Y+ NY++L+ AA   +E I LG PALD+AIT +  Y N  A A A +V+
Sbjct: 131 LFKGTQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHY-NAEAAASALLVL 190

Query: 181 IQTFAEAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQTPV 217
           IQT +EAARF+ I  +IA        KPS +++SLE  WSALSK IQ AK  +GQF+TPV
Sbjct: 191 IQTTSEAARFRYIELQIANNVGT-KFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPV 248

[Close]

BLAST of Moc07g03210 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 3.9e-36
Identity = 108/246 (43.90%), Postives = 143/246 (58.13%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MN  S F  +ILAIF      + G   ++FNLLG +SKSY NFI K LR ALPS ++VY+
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAG--DVSFNLLGSNSKSYSNFI-KALRSALPSKDKVYN 70

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           I +LL +     R+  + L+NYD + ITVA+DV NV                        
Sbjct: 71  ISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASK 130

Query: 121 -----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIV 180
                   + L YS NY+ L+ AA   +E+IPLGFPALD+AIT +  Y++  A A AFIV
Sbjct: 131 YVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDS-TAAAAAFIV 190

Query: 181 IIQTFAEAARFQSIMAE-IAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQT 217
           +IQ  AEA+R++ I A+ I  I+ D    PS +++SLE  WSALSK IQ A+TN G F+ 
Sbjct: 191 VIQCTAEASRYKYIEAQMIKRISKDD--VPSPAIISLENSWSALSKQIQLAQTNKGVFKA 250

[Close]

BLAST of Moc07g03210 vs. ExPASy TrEMBL
Match: A0A6J1H7R8 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111460858 PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 5.1e-36
Identity = 106/244 (43.44%), Postives = 139/244 (56.97%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MNK  A   + L IF+   P+      + F+L   +S SYK FI K LR ALP + +VY 
Sbjct: 1   MNKSLALSFLFLTIFLNTRPADAN---VRFDLSSATSSSYKTFI-KNLREALPKDGKVYD 60

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           IPVLL T+ D  RF+LI+L NYD ++IT A+DVLNV                        
Sbjct: 61  IPVLLSTVMDSRRFILIDLVNYDGQSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKL 120

Query: 121 ----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIVI 180
                +   L Y+ NY++L+ AA   +E I LG PALD+AIT +  Y N  A A A +V+
Sbjct: 121 LFKGTQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHY-NAEAAASALLVL 180

Query: 181 IQTFAEAARFQSIMAEIAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQTPV 217
           IQT +EAARF+ I  +IA        KPS +++SLE  WSALSK IQ AK  +GQF+TPV
Sbjct: 181 IQTTSEAARFRYIELQIANNVGT-KFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPV 238

[Close]

BLAST of Moc07g03210 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 5.1e-36
Identity = 108/246 (43.90%), Postives = 142/246 (57.72%), Query Frame = 0


Query: 1   MNKFSAFLSIILAIFVLDVPSIKGRDPLNFNLLGVSSKSYKNFIEKQLRPALPSNEQVYS 60
           MN  S F  +ILAIF      + G   ++FNLLG +SKSY NFIE  LR ALPS ++VY+
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAG--DVSFNLLGSNSKSYSNFIE-ALRSALPSKDKVYN 70

Query: 61  IPVLLPTIADKDRFLLINLANYDSETITVAVDVLNV------------------------ 120
           I +LL +     R+  + L+NYD + ITVA+DV NV                        
Sbjct: 71  ISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASK 130

Query: 121 -----PRHVDLDYSSNYKDLEGAAHASKEEIPLGFPALDAAITNMISYNNYRAVAKAFIV 180
                   + L YS NY+ L+ AA   +E+IPLGFPALD AIT +  Y++  A A AFIV
Sbjct: 131 YVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDS-TAAAAAFIV 190

Query: 181 IIQTFAEAARFQSIMAE-IAGIAADGSAKPSASMLSLETQWSALSKNIQYAKTNDGQFQT 217
           +IQ  AEA+R++ I A+ I  I+ D    PS +++SLE  WSALSK IQ A+TN G F+ 
Sbjct: 191 VIQCTAEASRYRYIEAQMIKRISKDD--VPSLAIISLENSWSALSKQIQLAQTNKGVFKA 250

The following BLAST results are available for this feature:

BLAST of Moc07g03210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150386.1	3.1e-112	100.00	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]
XP_038884329.1	2.1e-36	41.87	putative ribosome-inactivating protein [Benincasa hispida]	[more]
XP_023005049.1	4.7e-36	43.85	ribosome-inactivating protein cucurmosin [Cucurbita maxima]	[more]
XP_022992350.1	8.1e-36	43.90	ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima]	[more]
XP_022953306.1	1.1e-35	43.90	ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata]	[more]

BLAST of Moc07g03210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FRX4	5.3e-38	39.18	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]
P84531	2.0e-37	45.16	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
P16094	1.3e-36	42.28	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
P24478	8.4e-36	41.46	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
Q00465	1.4e-35	44.77	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of Moc07g03210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBC7	1.5e-112	100.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111018562 PE=3 SV=1	[more]
A0A6J1KY21	2.3e-36	43.85	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498157 PE=3 SV=1	[more]
A0A6J1JVG7	3.9e-36	43.90	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
A0A6J1H7R8	5.1e-36	43.44	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111460858 PE=3 SV=1	[more]
A0A6J1GMM2	5.1e-36	43.90	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]

BLAST of Moc07g03210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 102..199 e-value: 1.3E-16 score: 60.8
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 36..96 coord: 101..218
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 28..96 e-value: 6.9E-10 score: 40.9
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 97..161 e-value: 3.5E-15 score: 58.1
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 162..223 e-value: 4.8E-13 score: 50.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 284..310
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 284..308
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 36..96 coord: 101..218
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 29..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g03210.1	Moc07g03210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity