Moc07g02860 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g02860
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	cysteine proteinase inhibitor B
Location	chr7: 2528817 .. 2529203 (-)
RNA-Seq Expression	Moc07g02860
Synteny	Moc07g02860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGAAGAAGATTAATCGCGGCGGCGATCGCAATCGCGGCCGTGACGCTGGCGGCTGCGGCGGAAGGATACGGCGGAAGATTGGGCGGAAGGACGGAGATCGAAGACGTGAGAACGAACGAGGAGGTGCAGGAATTAGGGCGATTCTGCGTGGAGGAGTACAATCGGCGGAGGAACGGCGGCGGAGAGGTGAGATTCAGGGCGGTGGTGGCGGCGGAGAAGCAGGTGGTGGCCGGAATGAAGTATTATCTGAAGATCTCCGGGAGCGTGAAGGGGGAGAGTGAAATGTTGTTCGATTCGATCGTGATTGTGAAGCCCTGGCTTCGCTCTAAGCAACTCCTCCATTTTTCGCCCTCCACAATTTTGATCGCCAACAAATTTTGA

mRNA sequence

ATGGCGAGAAGAAGATTAATCGCGGCGGCGATCGCAATCGCGGCCGTGACGCTGGCGGCTGCGGCGGAAGGATACGGCGGAAGATTGGGCGGAAGGACGGAGATCGAAGACGTGAGAACGAACGAGGAGGTGCAGGAATTAGGGCGATTCTGCGTGGAGGAGTACAATCGGCGGAGGAACGGCGGCGGAGAGGTGAGATTCAGGGCGGTGGTGGCGGCGGAGAAGCAGGTGGTGGCCGGAATGAAGTATTATCTGAAGATCTCCGGGAGCGTGAAGGGGGAGAGTGAAATGTTGTTCGATTCGATCGTGATTGTGAAGCCCTGGCTTCGCTCTAAGCAACTCCTCCATTTTTCGCCCTCCACAATTTTGATCGCCAACAAATTTTGA

Coding sequence (CDS)

ATGGCGAGAAGAAGATTAATCGCGGCGGCGATCGCAATCGCGGCCGTGACGCTGGCGGCTGCGGCGGAAGGATACGGCGGAAGATTGGGCGGAAGGACGGAGATCGAAGACGTGAGAACGAACGAGGAGGTGCAGGAATTAGGGCGATTCTGCGTGGAGGAGTACAATCGGCGGAGGAACGGCGGCGGAGAGGTGAGATTCAGGGCGGTGGTGGCGGCGGAGAAGCAGGTGGTGGCCGGAATGAAGTATTATCTGAAGATCTCCGGGAGCGTGAAGGGGGAGAGTGAAATGTTGTTCGATTCGATCGTGATTGTGAAGCCCTGGCTTCGCTCTAAGCAACTCCTCCATTTTTCGCCCTCCACAATTTTGATCGCCAACAAATTTTGA

Protein sequence

MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRNGGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPSTILIANKF

Homology

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BLAST of Moc07g02860 vs. NCBI nr
Match: XP_022150412.1 (cysteine proteinase inhibitor B [Momordica charantia])

HSP 1 Score: 240.4 bits (612), Expect = 8.8e-60
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60
           MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN
Sbjct: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60

Query: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120
           GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS
Sbjct: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120

Query: 121 TILIANKF 129
           TILIANKF
Sbjct: 121 TILIANKF 128

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BLAST of Moc07g02860 vs. NCBI nr
Match: XP_004138536.1 (cysteine proteinase inhibitor B [Cucumis sativus] >KGN45922.1 hypothetical protein Csa_005586 [Cucumis sativus])

HSP 1 Score: 145.6 bits (366), Expect = 3.0e-31
Identity = 77/122 (63.11%), Postives = 96/122 (78.69%), Query Frame = 0


Query: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60
           MA+ +L +  + + AV L A  EGYGGR+GGR E++DVR NEEVQ LGRF VEEYNRR  
Sbjct: 1   MAKTKL-SVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG 60

Query: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120
           GGGEV+F AVVAAE+QVV+G KYYL+I G+  GE + +FDS+VIVKPW+ SK+LL FSPS
Sbjct: 61  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERK-VFDSVVIVKPWIGSKRLLDFSPS 120

Query: 121 TI 123
            +
Sbjct: 121 AV 120

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BLAST of Moc07g02860 vs. NCBI nr
Match: XP_008465470.1 (PREDICTED: cysteine proteinase inhibitor B [Cucumis melo])

HSP 1 Score: 143.3 bits (360), Expect = 1.5e-30
Identity = 77/122 (63.11%), Postives = 95/122 (77.87%), Query Frame = 0


Query: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60
           MA+ +L    +A AAV L    EGYGG++GGR EI+DV+ NEEVQ LGRF VEEYNRR  
Sbjct: 1   MAKTKLSVMLVA-AAVALVVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRRMG 60

Query: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120
           GGGEV+F AVVAA++QVV+G KYYL+I G   GE + +FDS+VIVKPW+ SK+LL FSPS
Sbjct: 61  GGGEVKFTAVVAADRQVVSGTKYYLRILGIQNGERK-IFDSVVIVKPWIGSKRLLDFSPS 120

Query: 121 TI 123
           T+
Sbjct: 121 TV 120

[Close]

BLAST of Moc07g02860 vs. NCBI nr
Match: XP_038886316.1 (cysteine proteinase inhibitor 4 [Benincasa hispida])

HSP 1 Score: 142.5 bits (358), Expect = 2.5e-30
Identity = 75/117 (64.10%), Postives = 94/117 (80.34%), Query Frame = 0


Query: 7   IAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRR-NGGGEV 66
           + AA+A+A V  A  AEGYGGR+GGR EI+DV+ NEEVQ LGRF VEEYN R   GGG+V
Sbjct: 8   VMAAVAVALVAAAVVAEGYGGRVGGRMEIKDVKRNEEVQRLGRFSVEEYNHRMGGGGGKV 67

Query: 67  RFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPSTI 123
           +F AV+AAE+QVV+G+KYYL+I G   GES+ +FDS+VIVKPW+ SK+LL FSPS +
Sbjct: 68  KFAAVIAAERQVVSGIKYYLRILGIQNGESK-IFDSVVIVKPWIGSKRLLDFSPSIV 123

[Close]

BLAST of Moc07g02860 vs. NCBI nr
Match: XP_023513886.1 (cysteine proteinase inhibitor B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 134.8 bits (338), Expect = 5.2e-28
Identity = 75/125 (60.00%), Postives = 96/125 (76.80%), Query Frame = 0


Query: 1   MARRRL-IAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNR-- 60
           M + RL + A + +A + +A  AEGY GR+GGR  I+DVR+NEEVQ LGRF VEEYNR  
Sbjct: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60

Query: 61  RRNGGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHF 120
           RR+G GEV+F AV+AAE+QVVAG KYYL+I GS  G  + +F+S+V+VKPWLRSKQL+ F
Sbjct: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGR-KRIFESVVVVKPWLRSKQLIDF 120

Query: 121 SPSTI 123
           S S +
Sbjct: 121 SASRL 124

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BLAST of Moc07g02860 vs. ExPASy Swiss-Prot
Match: Q5N806 (Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0915200 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.8e-21
Identity = 55/107 (51.40%), Postives = 77/107 (71.96%), Query Frame = 0


Query: 24  GYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRNGG--GEVR--FRAVVAAEKQVVA 83
           G G R+GGRTE+ DV  + EVQELGRF VEE+NRRR     G+VR  F  VVAA++QVV+
Sbjct: 45  GAGRRVGGRTEVRDVEGDREVQELGRFSVEEHNRRRRSRDCGDVRLEFGRVVAAQRQVVS 104

Query: 84  GMKYYLKISGSVKGES------EMLFDSIVIVKPWLRSKQLLHFSPS 121
           G+KYYL+++ + +G +        +FD++V+VKPWL S+ LL F+P+
Sbjct: 105 GLKYYLRVAAAEEGAAGQNGGEPRVFDAVVVVKPWLESRTLLTFAPA 151

[Close]

BLAST of Moc07g02860 vs. ExPASy Swiss-Prot
Match: Q0JGM8 (Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0915401 PE=3 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 3.5e-19
Identity = 58/132 (43.94%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 6   LIAAAIAIAAVTLAAAAE---------GYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYN 65
           L+ A ++ A +  AAAA+         G G ++GGRT++EDV  N EVQELG FCV E+N
Sbjct: 17  LLLAPLSSARLAAAAAADDDGQWPAGGGRGRKVGGRTDVEDVEGNREVQELGLFCVVEHN 76

Query: 66  RRRNGGGEVR-----FRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRS 124
           RR  GG   R     F  VVAA+ QVV+G+KYYL+I+   + + E++FD++V+VK W+ S
Sbjct: 77  RR--GGSATRGRGLVFSRVVAAQTQVVSGIKYYLRIAAQ-EADDELVFDAVVVVKAWVPS 136

[Close]

BLAST of Moc07g02860 vs. ExPASy Swiss-Prot
Match: Q10993 (Cysteine proteinase inhibitor B OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.5e-19
Identity = 46/97 (47.42%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 30  GGRTEIEDVRTNEEVQELGRFCVEEYNR----RRNGGGEVRFRAVVAAEKQVVAGMKYYL 89
           GGRT++++V+T+ E+Q+LG + V+EYNR    ++ G G+++F  V+AAE QVVAG KYYL
Sbjct: 3   GGRTKVKNVKTDTEIQQLGSYSVDEYNRLQRTKKTGAGDLKFSQVIAAETQVVAGTKYYL 62

Query: 90  KISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPSTI 123
           KI    KG    +FD+ V+V+ W  SK+LL F P+ +
Sbjct: 63  KIEAITKGGKMKVFDAEVVVQSWKHSKKLLGFKPAPV 99

[Close]

BLAST of Moc07g02860 vs. ExPASy Swiss-Prot
Match: Q8L5T9 (Cysteine proteinase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=CYS2 PE=2 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 7.9e-19
Identity = 47/106 (44.34%), Postives = 73/106 (68.87%), Query Frame = 0


Query: 29  LGGRTEIEDVRTNEEVQELGRFCVEEYNRR-RNGGGEV--------------RFRAVVAA 88
           LGG++ + ++RTN E+Q+LGR+CVE++N++ +N  G +              +F  VV+A
Sbjct: 36  LGGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQGNIGSIAKTDTAISNPLQFSRVVSA 95

Query: 89  EKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSP 120
           +KQVVAG+KYYL+I  +    S  +FDS+V+++PWL SKQLL F+P
Sbjct: 96  QKQVVAGLKYYLRIEVTQPNGSTRMFDSVVVIQPWLHSKQLLGFTP 141

[Close]

BLAST of Moc07g02860 vs. ExPASy Swiss-Prot
Match: A2XS65 (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_014908 PE=3 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 2.5e-17
Identity = 56/133 (42.11%), Postives = 80/133 (60.15%), Query Frame = 0


Query: 6   LIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRNGGG-- 65
           L+AAA    A    AAA      +GGRTEI DV +N+ VQ LGRF V E+NRR   GG  
Sbjct: 16  LVAAATGDEASPSNAAAPAAPVLVGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSG 75

Query: 66  --------EVRFRAVVAAEKQVVAGMKYYLKISGSVK-------GESEMLFDSIVIVKPW 122
                   ++ F  VV A+KQVV+G+ YYLK++ S +          + +FD++V+VK W
Sbjct: 76  GPADPVPVKLAFARVVEAQKQVVSGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAW 135

[Close]

BLAST of Moc07g02860 vs. ExPASy TrEMBL
Match: A0A6J1DAN3 (cysteine proteinase inhibitor B OS=Momordica charantia OX=3673 GN=LOC111018574 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.3e-60
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60
           MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN
Sbjct: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60

Query: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120
           GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS
Sbjct: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120

Query: 121 TILIANKF 129
           TILIANKF
Sbjct: 121 TILIANKF 128

[Close]

BLAST of Moc07g02860 vs. ExPASy TrEMBL
Match: A0A0A0KC20 (Cystatin OS=Cucumis sativus OX=3659 GN=Csa_6G022340 PE=4 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.4e-31
Identity = 77/122 (63.11%), Postives = 96/122 (78.69%), Query Frame = 0


Query: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60
           MA+ +L +  + + AV L A  EGYGGR+GGR E++DVR NEEVQ LGRF VEEYNRR  
Sbjct: 1   MAKTKL-SVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG 60

Query: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120
           GGGEV+F AVVAAE+QVV+G KYYL+I G+  GE + +FDS+VIVKPW+ SK+LL FSPS
Sbjct: 61  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERK-VFDSVVIVKPWIGSKRLLDFSPS 120

Query: 121 TI 123
            +
Sbjct: 121 AV 120

[Close]

BLAST of Moc07g02860 vs. ExPASy TrEMBL
Match: A0A1S3CPD1 (cysteine proteinase inhibitor B OS=Cucumis melo OX=3656 GN=LOC103503076 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.1e-31
Identity = 77/122 (63.11%), Postives = 95/122 (77.87%), Query Frame = 0


Query: 1   MARRRLIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRN 60
           MA+ +L    +A AAV L    EGYGG++GGR EI+DV+ NEEVQ LGRF VEEYNRR  
Sbjct: 1   MAKTKLSVMLVA-AAVALVVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRRMG 60

Query: 61  GGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 120
           GGGEV+F AVVAA++QVV+G KYYL+I G   GE + +FDS+VIVKPW+ SK+LL FSPS
Sbjct: 61  GGGEVKFTAVVAADRQVVSGTKYYLRILGIQNGERK-IFDSVVIVKPWIGSKRLLDFSPS 120

Query: 121 TI 123
           T+
Sbjct: 121 TV 120

[Close]

BLAST of Moc07g02860 vs. ExPASy TrEMBL
Match: A0A5N5HJ78 (Cysteine proteinase inhibitor B-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_025466 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.7e-27
Identity = 70/113 (61.95%), Postives = 88/113 (77.88%), Query Frame = 0


Query: 12  AIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRR-----NGGGEVR 71
           A+    L  A+ GYGG LGGR EIE+V+TNEEVQELGRF VEEYNR+R     +GGGE++
Sbjct: 8   ALVICLLFVASNGYGGMLGGRKEIENVKTNEEVQELGRFSVEEYNRQRGTQKMDGGGELQ 67

Query: 72  FRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSP 120
           F  VV A+ QVV+G+KYY+K+S +V+    MLFDS V+VKPWLRSKQLL+F+P
Sbjct: 68  FLEVVEAQSQVVSGLKYYIKVS-AVRNGVHMLFDSEVVVKPWLRSKQLLNFAP 119

[Close]

BLAST of Moc07g02860 vs. ExPASy TrEMBL
Match: A0A5N5H943 (Cysteine proteinase inhibitor B OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_025265 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 6.2e-27
Identity = 70/113 (61.95%), Postives = 88/113 (77.88%), Query Frame = 0


Query: 12  AIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRR-----NGGGEVR 71
           A+    L  A+ GYGG LGGR EIE+V+TN+EVQELGRF VEEYNR+R     +GGGE++
Sbjct: 8   ALVICLLFVASNGYGGMLGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMDGGGELQ 67

Query: 72  FRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSP 120
           F  VV A+ QVV+G+KYYLK+S +V+    MLFDS V+VKPWLRSKQLL+F+P
Sbjct: 68  FLEVVEAQSQVVSGLKYYLKVS-AVRNGVHMLFDSEVVVKPWLRSKQLLNFAP 119

[Close]

BLAST of Moc07g02860 vs. TAIR 10
Match: AT2G31980.1 (PHYTOCYSTATIN 2 )

HSP 1 Score: 94.7 bits (234), Expect = 5.6e-20
Identity = 47/106 (44.34%), Postives = 73/106 (68.87%), Query Frame = 0


Query: 29  LGGRTEIEDVRTNEEVQELGRFCVEEYNRR-RNGGGEV--------------RFRAVVAA 88
           LGG++ + ++RTN E+Q+LGR+CVE++N++ +N  G +              +F  VV+A
Sbjct: 36  LGGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQGNIGSIAKTDTAISNPLQFSRVVSA 95

Query: 89  EKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSP 120
           +KQVVAG+KYYL+I  +    S  +FDS+V+++PWL SKQLL F+P
Sbjct: 96  QKQVVAGLKYYLRIEVTQPNGSTRMFDSVVVIQPWLHSKQLLGFTP 141

[Close]

BLAST of Moc07g02860 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 32/93 (34.41%), Postives = 56/93 (60.22%), Query Frame = 0


Query: 29  LGGRTEIEDVRTNEEVQELGRFCVEEYNRRRNGGGEVRFRAVVAAEKQVVAGMKYYLKIS 88
           LGG  ++   + + E++ L RF ++E+N+++N    + F+ +V A +QVVAG  Y+L + 
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQN--KILEFKKIVKAREQVVAGTMYHLTLE 94

Query: 89  GSVKGESEMLFDSIVIVKPWLRSKQLLHFSPST 122
              +G+    F++ V VKPW+  KQL  F  S+
Sbjct: 95  AK-EGDQTKNFEAKVWVKPWMNFKQLQEFKESS 124

[Close]

BLAST of Moc07g02860 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 4.4e-09
Identity = 39/115 (33.91%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 6   LIAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNRRRNGGGEV 65
           L+  ++ +  + L A+A     R+GG + I +V T+ +V E+G F V EYN+R   G  +
Sbjct: 8   LLLLSLVVVLLPLYASA---AARVGGWSPISNV-TDPQVVEIGEFAVSEYNKRSESG--L 67

Query: 66  RFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHFSPS 121
           +F  VV+ E QVV+G  Y LK++ +        + +IV  KPW++ + L  F P+
Sbjct: 68  KFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEPA 116

[Close]

BLAST of Moc07g02860 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 57.4 bits (137), Expect = 9.9e-09
Identity = 33/93 (35.48%), Postives = 57/93 (61.29%), Query Frame = 0


Query: 29  LGGRTEIEDVRTNEEVQELGRFCVEEYNRRRNGGGEVRFRAVVAAEKQVVAGMKYYLKIS 88
           +GG  ++   + + EV+ L RF V+E+N++ N    + F  VV A++QVVAG  ++L + 
Sbjct: 37  VGGVGDVPANQNSGEVESLARFAVDEHNKKEN--ALLEFARVVKAKEQVVAGTLHHLTLE 96

Query: 89  GSVKGESEMLFDSIVIVKPWLRSKQLLHFSPST 122
               G+ + L+++ V VKPWL  K+L  F P++
Sbjct: 97  ILEAGQKK-LYEAKVWVKPWLNFKELQEFKPAS 126

[Close]

BLAST of Moc07g02860 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 57.4 bits (137), Expect = 9.9e-09
Identity = 33/93 (35.48%), Postives = 57/93 (61.29%), Query Frame = 0


Query: 29  LGGRTEIEDVRTNEEVQELGRFCVEEYNRRRNGGGEVRFRAVVAAEKQVVAGMKYYLKIS 88
           +GG  ++   + + EV+ L RF V+E+N++ N    + F  VV A++QVVAG  ++L + 
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNKKEN--ALLEFARVVKAKEQVVAGTLHHLTLE 63

Query: 89  GSVKGESEMLFDSIVIVKPWLRSKQLLHFSPST 122
               G+ + L+++ V VKPWL  K+L  F P++
Sbjct: 64  ILEAGQKK-LYEAKVWVKPWLNFKELQEFKPAS 93

The following BLAST results are available for this feature:

BLAST of Moc07g02860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150412.1	8.8e-60	100.00	cysteine proteinase inhibitor B [Momordica charantia]	[more]
XP_004138536.1	3.0e-31	63.11	cysteine proteinase inhibitor B [Cucumis sativus] >KGN45922.1 hypothetical prote...	[more]
XP_008465470.1	1.5e-30	63.11	PREDICTED: cysteine proteinase inhibitor B [Cucumis melo]	[more]
XP_038886316.1	2.5e-30	64.10	cysteine proteinase inhibitor 4 [Benincasa hispida]	[more]
XP_023513886.1	5.2e-28	60.00	cysteine proteinase inhibitor B [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc07g02860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5N806	3.8e-21	51.40	Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01...	[more]
Q0JGM8	3.5e-19	43.94	Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01...	[more]
Q10993	3.5e-19	47.42	Cysteine proteinase inhibitor B OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]
Q8L5T9	7.9e-19	44.34	Cysteine proteinase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=CYS2 PE=2 SV=...	[more]
A2XS65	2.5e-17	42.11	Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_0...	[more]

BLAST of Moc07g02860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAN3	4.3e-60	100.00	cysteine proteinase inhibitor B OS=Momordica charantia OX=3673 GN=LOC111018574 P...	[more]
A0A0A0KC20	1.4e-31	63.11	Cystatin OS=Cucumis sativus OX=3659 GN=Csa_6G022340 PE=4 SV=1	[more]
A0A1S3CPD1	7.1e-31	63.11	cysteine proteinase inhibitor B OS=Cucumis melo OX=3656 GN=LOC103503076 PE=4 SV=...	[more]
A0A5N5HJ78	3.7e-27	61.95	Cysteine proteinase inhibitor B-like OS=Pyrus ussuriensis x Pyrus communis OX=24...	[more]
A0A5N5H943	6.2e-27	61.95	Cysteine proteinase inhibitor B OS=Pyrus ussuriensis x Pyrus communis OX=2448454...	[more]

BLAST of Moc07g02860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31980.1	5.6e-20	44.34	PHYTOCYSTATIN 2	[more]
AT2G40880.1	2.0e-09	34.41	cystatin A	[more]
AT5G47550.1	4.4e-09	33.91	Cystatin/monellin superfamily protein	[more]
AT3G12490.2	9.9e-09	35.48	cystatin B	[more]
AT3G12490.1	9.9e-09	35.48	cystatin B	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 27..120 e-value: 3.0E-11 score: 53.4
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 37..118 e-value: 2.2E-13 score: 50.3
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 30..119 e-value: 1.027E-14 score: 63.262
None	No IPR available	GENE3D	3.10.450.10		coord: 27..121 e-value: 5.7E-27 score: 95.9
None	No IPR available	PANTHER	PTHR47373:SF1	CYSTEINE PROTEINASE INHIBITOR 2	coord: 9..121
None	No IPR available	PANTHER	PTHR47373	CYSTEINE PROTEINASE INHIBITOR 2	coord: 9..121
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 35..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g02860.1	Moc07g02860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity