Moc06g42720 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g42720
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr6: 33456776 .. 33457991 (+)
RNA-Seq Expression	Moc06g42720
Synteny	Moc06g42720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCCGGATCGGGCTGAGGTATATCCATCTCTTCCTAGTGCCAATTACACACAAAGCAGCTTTCTCGTTCATTAAGTAAATATTTTCTTTTTAAAATTTTATAAAACACACAATGAATAAGAACCCCGCCAGTTCCCAATTTGAGGACGATTTTCTTATCTTTTTATGAATAAGTAAAACGTCTAACTTTGATGGCTGTCAAAGAAGTCAAGATCTTGCAGTGTCAAATTAAATACTTTTGCCCTTTGTGTTGATACATCTCGCAGTATTGCTAATGGTTCAACTAGAAAAGGTGTTGCCACTTTAAAGAATTCGCTTACTGGAGCTTCTTCAATAGTGGATTGTCTTCCCATGCTGGATGGTGAACTGAATTATAGTCTAATTCCCAATTTATGTTTGTACTCTCCTTTTCCCCTATATTTTTCAACTTCCCGTATATTATAAAAATGTCAGATCTGAAGGCCCTAGTTTTGTTTTGAGAATCTGCTTTTGATATGCAGGCTTGAAGGCAAGATAGCACTGGTTACGGGAGCAGCAAGCGGTATTGGAAGACAACTGCGTCCAAATTTATAAACAACGGAGCCAAATTGTGATTTACGACGTCAGAGAAAAACTTGGCCTAGAGACGGCCGAGCAGCTAGGACCCAACGCAGCCTTCGTCCCCTGTGATGTCATGTTACTAAAGAGTCTGATGTCGCTGGTGCTGTCAATTACACTATCTCTCTACACAAAACGCTAGATATCATGTACAACAATGCTGGGGTTGCTTGCAACACTCCTCCAAGCATTTTGGACCTCGACCTCGTGGTGTTCGATAAAGTCATGAACATCAATGTCCGTGGAGTCCTGGCTGGTATCAAACATGCCTCCCGTGTCATGATCCCTCGACAATCAGGTCTATTTTCTGCGCAGCTAGCGTCACAGGACCGAGGGCTGGGCTGCCCCAACCCACGTACTCAGTGTCAAAGGTCGCTATTATATGCATTGTTACATCCATGGCATCAGAGTTGTGTGAATACGGTATCCGGGTCAATTGCATATCACCATTTCCTATCCCGACATCATTTGTTATGGAGGAAATGAGTCGCCTTTTTCCTTGTATTGAAGAGCAGAAACTTGAGAAGATGATATTCGACGTGGGTGCTTTAGAAGGAACAGTTTGTGAAACCAATGATATGGCAAATGCTGCCCTTTTTATTGCATCTGAATTCTGA

mRNA sequence

ATGTTCCGGATCGGGCTGAGTATTGCTAATGGTTCAACTAGAAAAGGTGTTGCCACTTTAAAGAATTCGCTTACTGGAGCTTCTTCAATAGTGGATTGTCTTCCCATGCTGGATGGTGAACTGAATTATAGTCTAATTCCCAATTTATGTTTGCTTGAAGGCAAGATAGCACTGGTTACGGGAGCAGCAAGCGATATCATGTACAACAATGCTGGGGTTGCTTGCAACACTCCTCCAAGCATTTTGGACCTCGACCTCGTGGTGTTCGATAAAGTCATGAACATCAATGTCCGTGGAGTCCTGGCTGCTAGCGTCACAGGACCGAGGGCTGGGCTGCCCCAACCCACGTACTCAGTGTCAAAGGTCGCTATTATATGCATTGTTACATCCATGGCATCAGAGTTGTGTGAATACGGTATCCGGGTCAATTGCATATCACCATTTCCTATCCCGACATCATTTGTTATGGAGGAAATGAGTCGCCTTTTTCCTTGTATTGAAGAGCAGAAACTTGAGAAGATGATATTCGACGTGGGTGCTTTAGAAGGAACAGTTTGTGAAACCAATGATATGGCAAATGCTGCCCTTTTTATTGCATCTGAATTCTGA

Coding sequence (CDS)

ATGTTCCGGATCGGGCTGAGTATTGCTAATGGTTCAACTAGAAAAGGTGTTGCCACTTTAAAGAATTCGCTTACTGGAGCTTCTTCAATAGTGGATTGTCTTCCCATGCTGGATGGTGAACTGAATTATAGTCTAATTCCCAATTTATGTTTGCTTGAAGGCAAGATAGCACTGGTTACGGGAGCAGCAAGCGATATCATGTACAACAATGCTGGGGTTGCTTGCAACACTCCTCCAAGCATTTTGGACCTCGACCTCGTGGTGTTCGATAAAGTCATGAACATCAATGTCCGTGGAGTCCTGGCTGCTAGCGTCACAGGACCGAGGGCTGGGCTGCCCCAACCCACGTACTCAGTGTCAAAGGTCGCTATTATATGCATTGTTACATCCATGGCATCAGAGTTGTGTGAATACGGTATCCGGGTCAATTGCATATCACCATTTCCTATCCCGACATCATTTGTTATGGAGGAAATGAGTCGCCTTTTTCCTTGTATTGAAGAGCAGAAACTTGAGAAGATGATATTCGACGTGGGTGCTTTAGAAGGAACAGTTTGTGAAACCAATGATATGGCAAATGCTGCCCTTTTTATTGCATCTGAATTCTGA

Protein sequence

MFRIGLSIANGSTRKGVATLKNSLTGASSIVDCLPMLDGELNYSLIPNLCLLEGKIALVTGAASDIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLAASVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFPCIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASEF

Homology

[Close]

BLAST of Moc06g42720 vs. NCBI nr
Match: XP_022154505.1 (secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154507.1 secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154508.1 secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154509.1 secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154510.1 secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154511.1 secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154512.1 secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154513.1 secoisolariciresinol dehydrogenase [Momordica charantia])

HSP 1 Score: 235.3 bits (599), Expect = 4.5e-58
Identity = 149/286 (52.10%), Postives = 160/286 (55.94%), Query Frame = 0


Query: 1   MFRIGLSIANGSTRKGVATLKNSLTGASSIVDCLPMLDGELNYSLIPNLCLLEGKIALVT 60
           MFRI LSIA+GSTRK V  L NSLT                N  L  +   LEGKIAL+T
Sbjct: 1   MFRIRLSIASGSTRKSVTNLTNSLT------------QSFFNRELSSHAGRLEGKIALIT 60

Query: 61  GAAS-------------------------------------------------------- 120
           GAAS                                                        
Sbjct: 61  GAASGIGKATASKFINNGAKVVIADVKEKLGLETAEQLGPNATYVPCDVTKESDVSDAVD 120

Query: 121 ---------DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVL-------------- 180
                    DIMYNNAGVACNTPPSI+DLDL VFDKVMN+NVRGVL              
Sbjct: 121 YTISLHKKLDIMYNNAGVACNTPPSIVDLDLAVFDKVMNVNVRGVLVGIKHASRVMIPRQ 180

Query: 181 ------AASVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFV 202
                  ASVTG   GL Q TYSVSKVAII +V SMASELC+YGIRVNCISPFPIPT FV
Sbjct: 181 SGSILCTASVTGLMGGLAQHTYSVSKVAIIGMVKSMASELCKYGIRVNCISPFPIPTPFV 240

[Close]

BLAST of Moc06g42720 vs. NCBI nr
Match: XP_038876474.1 (secoisolariciresinol dehydrogenase [Benincasa hispida])

HSP 1 Score: 220.3 bits (560), Expect = 1.5e-53
Identity = 112/157 (71.34%), Postives = 127/157 (80.89%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSILDLDL VFDKVMNINVRGVLA                    AS
Sbjct: 104 DIMYNNAGIACNTPPSILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPQRSGSILCTAS 163

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           +TG   GL Q TYSVSKVA+I IV S+ASELC+YGIRVNCISPFPIPT FV+EEM+RLFP
Sbjct: 164 ITGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMTRLFP 223

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
           C+E++KLEKMIFD+ ALEGTVCETND+ANAAL++AS+
Sbjct: 224 CVEKEKLEKMIFDLSALEGTVCETNDIANAALYLASD 260

[Close]

BLAST of Moc06g42720 vs. NCBI nr
Match: XP_008467261.1 (PREDICTED: secoisolariciresinol dehydrogenase [Cucumis melo])

HSP 1 Score: 213.8 bits (543), Expect = 1.4e-51
Identity = 110/157 (70.06%), Postives = 124/157 (78.98%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSI DLDL VFDKVMN+NVRGVLA                    AS
Sbjct: 110 DIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTAS 169

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           VTG   GL Q TYSVSKVA+I IV S+ASELC YGIRVNCISPFPIPT FV+EEM++LFP
Sbjct: 170 VTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFP 229

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            +EE+KLEKMIFD+ ALEGTVCETND+ANAAL++AS+
Sbjct: 230 SVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASD 266

[Close]

BLAST of Moc06g42720 vs. NCBI nr
Match: KAA0055139.1 (secoisolariciresinol dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 213.8 bits (543), Expect = 1.4e-51
Identity = 110/157 (70.06%), Postives = 124/157 (78.98%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSI DLDL VFDKVMN+NVRGVLA                    AS
Sbjct: 80  DIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTAS 139

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           VTG   GL Q TYSVSKVA+I IV S+ASELC YGIRVNCISPFPIPT FV+EEM++LFP
Sbjct: 140 VTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFP 199

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            +EE+KLEKMIFD+ ALEGTVCETND+ANAAL++AS+
Sbjct: 200 SVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASD 236

[Close]

BLAST of Moc06g42720 vs. NCBI nr
Match: TYK20720.1 (secoisolariciresinol dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 213.8 bits (543), Expect = 1.4e-51
Identity = 110/157 (70.06%), Postives = 124/157 (78.98%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSI DLDL VFDKVMN+NVRGVLA                    AS
Sbjct: 83  DIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTAS 142

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           VTG   GL Q TYSVSKVA+I IV S+ASELC YGIRVNCISPFPIPT FV+EEM++LFP
Sbjct: 143 VTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFP 202

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            +EE+KLEKMIFD+ ALEGTVCETND+ANAAL++AS+
Sbjct: 203 SVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASD 239

[Close]

BLAST of Moc06g42720 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 59/164 (35.98%), Postives = 84/164 (51.22%), Query Frame = 0


Query: 65  DIMYNNAGVACNTP--PSILDLDLVVFDKVMNINVRGV---------------------L 124
           DI++NNAGV  +     SILD D   FD VM +NVRGV                      
Sbjct: 115 DILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIIS 174

Query: 125 AASVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSR 184
            ASV G   G+    Y+ SK AI+ +  + A EL +YGIRVNCISPF + TS ++    +
Sbjct: 175 TASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRK 234

Query: 185 LFPCIEE----QKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
                 E    +++E+ +  +  L+G     ND+A AAL++AS+
Sbjct: 235 TSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAEAALYLASD 278

[Close]

BLAST of Moc06g42720 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.3e-15
Identity = 52/158 (32.91%), Postives = 85/158 (53.80%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVL--------------------AAS 124
           DIM++NAG++    P I+D +   F++V ++NV GV                      AS
Sbjct: 94  DIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLCMKHAARVMIPARSGNIISTAS 153

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           ++    G     Y  SK A++ +  ++A EL ++GIRVNC+SPF +PT+     + + F 
Sbjct: 154 LSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNCLSPFGLPTA-----LGKKFS 213

Query: 185 CIE-EQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            I+ E++ E +I   G L+G      D+ANAAL++AS+
Sbjct: 214 GIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASD 246

[Close]

BLAST of Moc06g42720 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.7e-13
Identity = 45/158 (28.48%), Postives = 77/158 (48.73%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLAASVTGPRAGLP----------- 124
           DIM+ N GV   TP SIL+     F +VM+INV G    +    R  +P           
Sbjct: 94  DIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLVAKHAARVMIPAKKGSIVFTAS 153

Query: 125 ----------QPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLF 184
                        Y+ +K A++ + TS+ +EL EYGIRVNC+SP+ + +  + +      
Sbjct: 154 ISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRVNCVSPYIVASPLLTDVFG--- 213

Query: 185 PCIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
             ++  ++E++      L+GT+    D+A+A  ++A +
Sbjct: 214 --VDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGD 246

[Close]

BLAST of Moc06g42720 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.6e-12
Identity = 42/158 (26.58%), Postives = 76/158 (48.10%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLAASVTGPRAGLP----------- 124
           DIM+ N GV   TP SIL+     F +VM+INV G    +    R  +P           
Sbjct: 94  DIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLVAKHAARVMIPAKKGSIVFTAS 153

Query: 125 ----------QPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLF 184
                        Y+ +K A++ + TS+ +EL ++GIRVNC+SP+ + +  + +      
Sbjct: 154 ISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRVNCVSPYVVASPLLTDVFG--- 213

Query: 185 PCIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
             ++  ++E++      L+G +    D+A+A  ++A +
Sbjct: 214 --VDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGD 246

[Close]

BLAST of Moc06g42720 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.6e-12
Identity = 52/165 (31.52%), Postives = 78/165 (47.27%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVL--------------------AAS 124
           DIM NNAG+  +    I D D   F KV +INV GV                      AS
Sbjct: 83  DIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLGMKHAARIMIPKMKGSIVSLAS 142

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           V+   AG     Y+ +K A++ +  S+A+EL  +GIRVNC+SP+ +PT   M       P
Sbjct: 143 VSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVNCVSPYAVPTRLSM-------P 202

Query: 185 CIEEQKLEK--------MIFDVGALEGTVCETNDMANAALFIASE 202
            + E ++++         +     L+G     ND+A A L++A+E
Sbjct: 203 YLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATE 240

[Close]

BLAST of Moc06g42720 vs. ExPASy TrEMBL
Match: A0A6J1DLW0 (secoisolariciresinol dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021770 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 2.2e-58
Identity = 149/286 (52.10%), Postives = 160/286 (55.94%), Query Frame = 0


Query: 1   MFRIGLSIANGSTRKGVATLKNSLTGASSIVDCLPMLDGELNYSLIPNLCLLEGKIALVT 60
           MFRI LSIA+GSTRK V  L NSLT                N  L  +   LEGKIAL+T
Sbjct: 1   MFRIRLSIASGSTRKSVTNLTNSLT------------QSFFNRELSSHAGRLEGKIALIT 60

Query: 61  GAAS-------------------------------------------------------- 120
           GAAS                                                        
Sbjct: 61  GAASGIGKATASKFINNGAKVVIADVKEKLGLETAEQLGPNATYVPCDVTKESDVSDAVD 120

Query: 121 ---------DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVL-------------- 180
                    DIMYNNAGVACNTPPSI+DLDL VFDKVMN+NVRGVL              
Sbjct: 121 YTISLHKKLDIMYNNAGVACNTPPSIVDLDLAVFDKVMNVNVRGVLVGIKHASRVMIPRQ 180

Query: 181 ------AASVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFV 202
                  ASVTG   GL Q TYSVSKVAII +V SMASELC+YGIRVNCISPFPIPT FV
Sbjct: 181 SGSILCTASVTGLMGGLAQHTYSVSKVAIIGMVKSMASELCKYGIRVNCISPFPIPTPFV 240

[Close]

BLAST of Moc06g42720 vs. ExPASy TrEMBL
Match: A0A5D3DB97 (Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001200 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 6.8e-52
Identity = 110/157 (70.06%), Postives = 124/157 (78.98%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSI DLDL VFDKVMN+NVRGVLA                    AS
Sbjct: 83  DIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTAS 142

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           VTG   GL Q TYSVSKVA+I IV S+ASELC YGIRVNCISPFPIPT FV+EEM++LFP
Sbjct: 143 VTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFP 202

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            +EE+KLEKMIFD+ ALEGTVCETND+ANAAL++AS+
Sbjct: 203 SVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASD 239

[Close]

BLAST of Moc06g42720 vs. ExPASy TrEMBL
Match: A0A5A7UNV0 (Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00700 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 6.8e-52
Identity = 110/157 (70.06%), Postives = 124/157 (78.98%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSI DLDL VFDKVMN+NVRGVLA                    AS
Sbjct: 80  DIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTAS 139

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           VTG   GL Q TYSVSKVA+I IV S+ASELC YGIRVNCISPFPIPT FV+EEM++LFP
Sbjct: 140 VTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFP 199

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            +EE+KLEKMIFD+ ALEGTVCETND+ANAAL++AS+
Sbjct: 200 SVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASD 236

[Close]

BLAST of Moc06g42720 vs. ExPASy TrEMBL
Match: A0A1S3CT49 (secoisolariciresinol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504658 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 6.8e-52
Identity = 110/157 (70.06%), Postives = 124/157 (78.98%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSI DLDL VFDKVMN+NVRGVLA                    AS
Sbjct: 110 DIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTAS 169

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           VTG   GL Q TYSVSKVA+I IV S+ASELC YGIRVNCISPFPIPT FV+EEM++LFP
Sbjct: 170 VTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFP 229

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            +EE+KLEKMIFD+ ALEGTVCETND+ANAAL++AS+
Sbjct: 230 SVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASD 266

[Close]

BLAST of Moc06g42720 vs. ExPASy TrEMBL
Match: A0A0A0KL23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G171720 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.6e-51
Identity = 111/157 (70.70%), Postives = 122/157 (77.71%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------AS 124
           DIMYNNAG+ACNTPPSI DLDL VFDKVMN+NVRGVLA                    AS
Sbjct: 110 DIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGVKHASRVMIPQRSGSILCTAS 169

Query: 125 VTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLFP 184
           VTG   GL Q TYSVSKVA+I IV S+ASELC YGIRVNCISPFPIPT FV+EEM +LFP
Sbjct: 170 VTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMVQLFP 229

Query: 185 CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
            +EE KLEKMIFD+ ALEGTVCETND+ANAALF+AS+
Sbjct: 230 RVEEAKLEKMIFDLSALEGTVCETNDIANAALFLASD 266

[Close]

BLAST of Moc06g42720 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 2.0e-40
Identity = 96/228 (42.11%), Postives = 131/228 (57.46%), Query Frame = 0


Query: 8   IANGSTRKGVATLKNSLTGASSIV--DCLPMLDGELNYSLIPNLCLLEGKIALVTGAAS- 67
           I  G++  G AT    ++  + ++  D  P +  E    L P+       +   +  A+ 
Sbjct: 85  ITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGPSCAYFPCDVTKESDIANA 144

Query: 68  -----------DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRGVL------------ 127
                      DIMYNNAG+ C TPPSI+DLDL VFDKV+N NVRGV+            
Sbjct: 145 VDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRGVMAGIKHAARVMIP 204

Query: 128 --------AASVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTS 187
                   A SVTG   GL Q TYSVSK A+I IV S ASELC++ IRVNCISPF I TS
Sbjct: 205 RNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRIRVNCISPFAITTS 264

Query: 188 FVMEEMSRLFPCIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
           FVM+EM +++P +++ +L +++   G L G VCE  D+ANAA+++AS+
Sbjct: 265 FVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLASD 312

[Close]

BLAST of Moc06g42720 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-25
Identity = 64/157 (40.76%), Postives = 91/157 (57.96%), Query Frame = 0


Query: 65  DIMYNNAG-VACNTPPSILDLDLVVFDKVMNINVRGVLA--------------------A 124
           D+MYNNAG V   TP SI  LD+  F++VM INV GV++                    +
Sbjct: 119 DVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVVSGIKHAAKFMIPARSGCILCTS 178

Query: 125 SVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLF 184
           SV G   GL   +Y++SK     IV S ASELCE+G+R+NCISP  + T   +  + ++F
Sbjct: 179 SVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVRINCISPGTVATPLTLSYLQKVF 238

Query: 185 PCIEEQKLEKMIFDVGALEGTVCETNDMANAALFIAS 201
           P + E+KL + +  +G L+G  CE  D+A AAL++AS
Sbjct: 239 PKVSEEKLRETVKGMGELKGAECEEADVAKAALYLAS 275

[Close]

BLAST of Moc06g42720 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 2.0e-24
Identity = 62/160 (38.75%), Postives = 93/160 (58.13%), Query Frame = 0


Query: 65  DIMYNNAGVACN-TPPSILDLDLVVFDKVMNINVRGVL--------------------AA 124
           D+M N+AG++C+ +PPSI DLD+  +DKVM +NVRG +                     +
Sbjct: 114 DVMLNSAGISCSISPPSIADLDMDTYDKVMRLNVRGTVLGIKHAARAMIPAGSGSILCLS 173

Query: 125 SVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLF 184
           S++G   GL    YS+SK  I  +V ++ASELC++G+R+NCISP  IPT   +      F
Sbjct: 174 SISGLMGGLGPHAYSISKFTIPGVVKTVASELCKHGLRINCISPAGIPTPLTLRMFREAF 233

Query: 185 P--CIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
               I E++L  ++   G L+G  CE  D+A AAL++AS+
Sbjct: 234 AGHSIREEQLLAIVNATGELKGEKCEEIDVAKAALYLASD 273

[Close]

BLAST of Moc06g42720 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 59/164 (35.98%), Postives = 84/164 (51.22%), Query Frame = 0


Query: 65  DIMYNNAGVACNTP--PSILDLDLVVFDKVMNINVRGV---------------------L 124
           DI++NNAGV  +     SILD D   FD VM +NVRGV                      
Sbjct: 115 DILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIIS 174

Query: 125 AASVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSR 184
            ASV G   G+    Y+ SK AI+ +  + A EL +YGIRVNCISPF + TS ++    +
Sbjct: 175 TASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRK 234

Query: 185 LFPCIEE----QKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
                 E    +++E+ +  +  L+G     ND+A AAL++AS+
Sbjct: 235 TSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAEAALYLASD 278

[Close]

BLAST of Moc06g42720 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 5.2e-12
Identity = 49/158 (31.01%), Postives = 79/158 (50.00%), Query Frame = 0


Query: 65  DIMYNNAGVACNTPPSILDLDLVVFDKVMNINVRG---------------------VLAA 124
           D++++NAGV      S+LDLDL  FD+ M +NVRG                     V   
Sbjct: 123 DVLFSNAGV-LEAFGSVLDLDLEAFDRTMAVNVRGAAAFIKHAARSMVASGTRGSIVCTT 182

Query: 125 SVTGPRAGLPQPTYSVSKVAIICIVTSMASELCEYGIRVNCISPFPIPTSFVMEEMSRLF 184
           S+     G    +Y+ SK A++ ++ S  + L +YGIRVN ++P+ + T      M+  +
Sbjct: 183 SIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVNGVAPYGVATG-----MTSAY 242

Query: 185 PCIEEQKLEKMIFDVGALEGTVCETNDMANAALFIASE 202
                + LE+    +G L+G V +   +A AALF+AS+
Sbjct: 243 NEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASD 274

The following BLAST results are available for this feature:

BLAST of Moc06g42720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154505.1	4.5e-58	52.10	secoisolariciresinol dehydrogenase [Momordica charantia] >XP_022154507.1 secoiso...	[more]
XP_038876474.1	1.5e-53	71.34	secoisolariciresinol dehydrogenase [Benincasa hispida]	[more]
XP_008467261.1	1.4e-51	70.06	PREDICTED: secoisolariciresinol dehydrogenase [Cucumis melo]	[more]
KAA0055139.1	1.4e-51	70.06	secoisolariciresinol dehydrogenase [Cucumis melo var. makuwa]	[more]
TYK20720.1	1.4e-51	70.06	secoisolariciresinol dehydrogenase [Cucumis melo var. makuwa]	[more]

BLAST of Moc06g42720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCU0	1.5e-16	35.98	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
Q94KL7	8.3e-15	32.91	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q94KL8	2.7e-13	28.48	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	8.6e-12	26.58	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
F1SWA0	8.6e-12	31.52	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of Moc06g42720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLW0	2.2e-58	52.10	secoisolariciresinol dehydrogenase OS=Momordica charantia OX=3673 GN=LOC11102177...	[more]
A0A5D3DB97	6.8e-52	70.06	Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A5A7UNV0	6.8e-52	70.06	Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3CT49	6.8e-52	70.06	secoisolariciresinol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504658 PE=4 ...	[more]
A0A0A0KL23	2.6e-51	70.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G171720 PE=4 SV=1	[more]

BLAST of Moc06g42720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03140.1	2.0e-40	42.11	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	1.1e-25	40.76	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26760.1	2.0e-24	38.75	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	1.1e-17	35.98	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.1	5.2e-12	31.01	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 62..73 score: 42.09 coord: 138..155 score: 38.38 coord: 181..201 score: 24.53 coord: 117..136 score: 31.45
None	No IPR available	GENE3D	3.40.50.720		coord: 63..202 e-value: 1.7E-19 score: 71.9
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 102..201 e-value: 2.8E-12 score: 46.7
None	No IPR available	PANTHER	PTHR43180:SF72	SECOISOLARICIRESINOL DEHYDROGENASE-LIKE	coord: 102..201 coord: 65..102
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 102..201
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 65..102
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 104..132
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 53..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g42720.1	Moc06g42720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity