Moc06g40380 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g40380
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Pectinesterase
Location	chr6: 31604313 .. 31606631 (+)
RNA-Seq Expression	Moc06g40380
Synteny	Moc06g40380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAGAGCTCTTGTAGCAGAGACCCTCACGAAGCAGCTCGGCCAAAAATGCCGAAACCAAAAGGAGAGAGCCGCGTGGGCCGATTGCGTGGAGCTTTTCCAATCCACAATCCTCCACCTCAACAAAACAGTCTCCGACAAATGCTCTGGTTTCGATATTCAGACATGGCTCAGCTCCGCACTCACCAACCTCGACACGTGTCGCGCTGGGTTTGCAGACCTCCACGTGGCGGACTACGGCGCCGTTTTTCCGCTGTTGGCGAATAATAACGTCACCAAGCTTATAAGCAACAGTTTGGCGATGAATAACTGCTCGGAATCCGAATCCGAACTGGGGGCAGGTTATAGAGGCGGGTTTCCGACATGGGTTCGGGGTGGGGACCGGCGGCTGCTGCAGGCGGCATCGCCGAAGGCGGATGTGGTGGTTGCGCAGGATGGGTCCGGGAACTACCGGACGATTGCGGCGGCGTTGGCGGCGGCGGAGAAGAGGAGCGGGAGTGGGAGATTTGTGATACATGTGAAGAGAGGGGTTTATAAGGAGAATTTGGTGATCGGAAGTAAGTTGAAGAATATAATGCTTTTGGGGGATGGATTGAGGTACACCATTATTACTGGGAGTAGAAGCGTTGGAGGAGGTTCCACCACTTTCAATTCTGCTACTGTTGGTCAGTTTCTCTCTCCTTTCTTTCCTTTTTTTTTTCCCTCTCCCTAAAAATTATAAAAAATACGTTTAAATCTCATTTTGTCTCCCTCCAAACACCATTTTATTTTTGTTCCAAATTTATAAAATGTCTATTTAGCTATTTTAATTTTTAAATGTTCAATATAGCTTTATTTTAGTCTTTCAACCTTAGAAAATGATATTTAATTTCTTTGCCATTATTTTGTGATTTAATATCTATCAACAAAAATACTGTGATCATCTTAATGGACTTGTATGTTAACAATAATACACATATTCAAAATCATGTTATCTATTCGTTAGGTTAAAGATTAATGGTTATTTTTTAAAAAGAAAAATAAGTTTTAGGATTAAAATTAACATTTTGAAAATTGAGGGACAAAAAATAGAATAAACCTAAAGAAAGACTATAATAAATATTTTAAAAGTTCAGAACTAAAATGGAATAATCCCACCGGCTTGAGACTTGAGCTTAAAATAATTATTTTAGTTTTTAAATTTTGAAGATGAATCAATCCTTAAAATAAAAATATTTATTTTAGTCGTCTAAATTTTTGAAGAATAAAACAAGATCATTTTAGTCCTTCCTATTAATAGTTTACCTATATAACTCCATATTGAAACATTAATACAATAATTATACATTTGATAAAAAAAAGTAAAATAAAGACATTTTAGATTATGCAATAGTTCAACTGGCCAGACATTGTAACGTTTAGTGAACTCTATCCCTAAATTTTTATCAGATTCAGAGGAGTTGTGTTGATGATCATATTTCTCTATTGAGTTAGCTTGAAAATTACAAAAAGAAATCTTATTTTCACATTCTTGAAACCTTTTTCCTGATTTGACAAATTCTCACGTTCACATTTTTCTCTTCCTTTTAGCAACTCTTTTGTAAATGCCTAATATATAACACTTGAGATCTTGTGACTTTCTTTAGCTGTGACTGGAGAACGTTTCATTGCTCGTGGTATCACATTCCGCAACACTGCCGGACCTCAAAACCACCAAGCAGTAGCGCTTCGGTCAGGCTCCGATCTCTCTGTCTTCTTTCAATGCAGTTTCGAGGGATATCAAGACACTCTCTACGTCCATTCTCAAAGACAATTCTACAGAGAGTGCTACATTTACGGCACGGTCGATTTCATCTTCGGTAATGCTGCTGTGGTCCTCCAAAATTGCATGATATACGCAAGAAAACCTATCCCTGGCCAGCAATGCGTCGTGACCGCCCAAGGCCGCACGGACCCGAACCAAAACACCGGGATCTCGATCCACAACTCGAGGGTGATGGCTGCAGCCGACCTCAAACCGGTTCAAAGCTCGGTTAGAACCTACTTGGGAAGGCCATGGAAGGAGTACTCTCGCACAGTTTACTTGAAAAATTACTTGGATGGGTTGGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCCCTTAATACATTGTACTATGGAGAGTATAACAATTTTGGGCCTGGTTCTTCTACTAGCAACAGAGTGAAATGGAAGGGTTATAGAGTAATTACAAGTGCCACAGAAGCATCAAAGTTTACTGTGGAGAATTTTATTGCTGGGAAGTCATGGCTGCCTGGGACTGGAGTGCCATTCACTGTTGGCCTTTGA

mRNA sequence

ATGGACAGAGCTCTTGTAGCAGAGACCCTCACGAAGCAGCTCGGCCAAAAATGCCGAAACCAAAAGGAGAGAGCCGCGTGGGCCGATTGCGTGGAGCTTTTCCAATCCACAATCCTCCACCTCAACAAAACAGTCTCCGACAAATGCTCTGGTTTCGATATTCAGACATGGCTCAGCTCCGCACTCACCAACCTCGACACGTGTCGCGCTGGGTTTGCAGACCTCCACGTGGCGGACTACGGCGCCGTTTTTCCGCTGTTGGCGAATAATAACGTCACCAAGCTTATAAGCAACAGTTTGGCGATGAATAACTGCTCGGAATCCGAATCCGAACTGGGGGCAGGTTATAGAGGCGGGTTTCCGACATGGGTTCGGGGTGGGGACCGGCGGCTGCTGCAGGCGGCATCGCCGAAGGCGGATGTGGTGGTTGCGCAGGATGGGTCCGGGAACTACCGGACGATTGCGGCGGCGTTGGCGGCGGCGGAGAAGAGGAGCGGGAGTGGGAGATTTGTGATACATGTGAAGAGAGGGGTTTATAAGGAGAATTTGGTGATCGGAAGTAAGTTGAAGAATATAATGCTTTTGGGGGATGGATTGAGGTACACCATTATTACTGGGAGTAGAAGCGTTGGAGGAGGTTCCACCACTTTCAATTCTGCTACTGTTGCTGTGACTGGAGAACGTTTCATTGCTCGTGGTATCACATTCCGCAACACTGCCGGACCTCAAAACCACCAAGCAGTAGCGCTTCGGTCAGGCTCCGATCTCTCTGTCTTCTTTCAATGCAGTTTCGAGGGATATCAAGACACTCTCTACGTCCATTCTCAAAGACAATTCTACAGAGAGTGCTACATTTACGGCACGGTCGATTTCATCTTCGGTAATGCTGCTGTGGTCCTCCAAAATTGCATGATATACGCAAGAAAACCTATCCCTGGCCAGCAATGCGTCGTGACCGCCCAAGGCCGCACGGACCCGAACCAAAACACCGGGATCTCGATCCACAACTCGAGGGTGATGGCTGCAGCCGACCTCAAACCGGTTCAAAGCTCGGTTAGAACCTACTTGGGAAGGCCATGGAAGGAGTACTCTCGCACAGTTTACTTGAAAAATTACTTGGATGGGTTGGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCCCTTAATACATTGTACTATGGAGAGTATAACAATTTTGGGCCTGGTTCTTCTACTAGCAACAGAGTGAAATGGAAGGGTTATAGAGTAATTACAAGTGCCACAGAAGCATCAAAGTTTACTGTGGAGAATTTTATTGCTGGGAAGTCATGGCTGCCTGGGACTGGAGTGCCATTCACTGTTGGCCTTTGA

Coding sequence (CDS)

ATGGACAGAGCTCTTGTAGCAGAGACCCTCACGAAGCAGCTCGGCCAAAAATGCCGAAACCAAAAGGAGAGAGCCGCGTGGGCCGATTGCGTGGAGCTTTTCCAATCCACAATCCTCCACCTCAACAAAACAGTCTCCGACAAATGCTCTGGTTTCGATATTCAGACATGGCTCAGCTCCGCACTCACCAACCTCGACACGTGTCGCGCTGGGTTTGCAGACCTCCACGTGGCGGACTACGGCGCCGTTTTTCCGCTGTTGGCGAATAATAACGTCACCAAGCTTATAAGCAACAGTTTGGCGATGAATAACTGCTCGGAATCCGAATCCGAACTGGGGGCAGGTTATAGAGGCGGGTTTCCGACATGGGTTCGGGGTGGGGACCGGCGGCTGCTGCAGGCGGCATCGCCGAAGGCGGATGTGGTGGTTGCGCAGGATGGGTCCGGGAACTACCGGACGATTGCGGCGGCGTTGGCGGCGGCGGAGAAGAGGAGCGGGAGTGGGAGATTTGTGATACATGTGAAGAGAGGGGTTTATAAGGAGAATTTGGTGATCGGAAGTAAGTTGAAGAATATAATGCTTTTGGGGGATGGATTGAGGTACACCATTATTACTGGGAGTAGAAGCGTTGGAGGAGGTTCCACCACTTTCAATTCTGCTACTGTTGCTGTGACTGGAGAACGTTTCATTGCTCGTGGTATCACATTCCGCAACACTGCCGGACCTCAAAACCACCAAGCAGTAGCGCTTCGGTCAGGCTCCGATCTCTCTGTCTTCTTTCAATGCAGTTTCGAGGGATATCAAGACACTCTCTACGTCCATTCTCAAAGACAATTCTACAGAGAGTGCTACATTTACGGCACGGTCGATTTCATCTTCGGTAATGCTGCTGTGGTCCTCCAAAATTGCATGATATACGCAAGAAAACCTATCCCTGGCCAGCAATGCGTCGTGACCGCCCAAGGCCGCACGGACCCGAACCAAAACACCGGGATCTCGATCCACAACTCGAGGGTGATGGCTGCAGCCGACCTCAAACCGGTTCAAAGCTCGGTTAGAACCTACTTGGGAAGGCCATGGAAGGAGTACTCTCGCACAGTTTACTTGAAAAATTACTTGGATGGGTTGGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCCCTTAATACATTGTACTATGGAGAGTATAACAATTTTGGGCCTGGTTCTTCTACTAGCAACAGAGTGAAATGGAAGGGTTATAGAGTAATTACAAGTGCCACAGAAGCATCAAAGTTTACTGTGGAGAATTTTATTGCTGGGAAGTCATGGCTGCCTGGGACTGGAGTGCCATTCACTGTTGGCCTTTGA

Protein sequence

MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDKCSGFDIQTWLSSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRGGFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFTVGL

Homology

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BLAST of Moc06g40380 vs. NCBI nr
Match: XP_038875884.1 (pectinesterase 2-like [Benincasa hispida])

HSP 1 Score: 788.1 bits (2034), Expect = 4.0e-224
Identity = 390/455 (85.71%), Postives = 418/455 (91.87%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSD-KCSGFDIQTWLS 60
           MDRA+ AETLTK+LG+KCRN+KERAAWADC+EL+Q+TILHLNKT SD KCS FDIQTWLS
Sbjct: 71  MDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKCSDFDIQTWLS 130

Query: 61  SALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRG- 120
           SALT+L+TCRAGF DL + DY  VFP LANNNVTKLISNSLAMNNCSES+     GY+  
Sbjct: 131 SALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESD----VGYKSN 190

Query: 121 -GFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRG 180
            GFP WVR GDRRLLQAA PKAD+VVAQDGSGNYRT+ AA+ AA KR GSGRFVIH+KRG
Sbjct: 191 EGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIHIKRG 250

Query: 181 VYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFR 240
           VYKEN+VI  K+KN+ML+GDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFR
Sbjct: 251 VYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFR 310

Query: 241 NTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAA 300
           NTAGP+NHQAVALRSGSDLSVFF+C FEGYQDTLY HSQRQFYRECYIYGTVDFIFGNAA
Sbjct: 311 NTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGNAA 370

Query: 301 VVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLG 360
           VVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV  SVRTYLG
Sbjct: 371 VVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTYLG 430

Query: 361 RPWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGY 420
           RPWKEYSRTVYL+N++D LVNPAGWLEWSGNFALNTLYYGE+ NFGPGSST+ RVKWKGY
Sbjct: 431 RPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGY 490

Query: 421 RVITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           RVITSATEASKFTV NFIAG+SWLPGTGVPFT GL
Sbjct: 491 RVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 519

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BLAST of Moc06g40380 vs. NCBI nr
Match: XP_008436965.1 (PREDICTED: pectinesterase 2-like [Cucumis melo])

HSP 1 Score: 778.1 bits (2008), Expect = 4.2e-221
Identity = 380/452 (84.07%), Postives = 412/452 (91.15%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDKCSGFDIQTWLSS 60
           M+RA+  ETLTK LGQKCRN+KERAAWADC+EL+Q+TILHLNKT SD CS FDIQTWLSS
Sbjct: 72  MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHCSNFDIQTWLSS 131

Query: 61  ALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRGGF 120
           ALTNLDTCRAGF DL + DYG VFP LANNN+TKLISNSLAMNNCSES+   G     GF
Sbjct: 132 ALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESDE--GNTSDEGF 191

Query: 121 PTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGVYK 180
           P WVRGGDRRLLQAA PKAD+VVAQDGSGNY+T+ AA+ AA KR GSGRFVI +K+GVYK
Sbjct: 192 PKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYK 251

Query: 181 ENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 240
           EN+VI  K+KN+ML+GDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA
Sbjct: 252 ENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 311

Query: 241 GPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVL 300
           GPQNHQAVALRSG+DLSVFF+C FEGYQDTLY HSQRQFYRECYIYGT+DFIFGNAAVVL
Sbjct: 312 GPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVVL 371

Query: 301 QNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGRPW 360
           QNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV  SVRTYLGRPW
Sbjct: 372 QNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRPW 431

Query: 361 KEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYRVI 420
           KEYSRTVYL+++++ LVNPAGWLEWSGNFALNTLYYGE+ N+GPGSST+NRVKWKGYRVI
Sbjct: 432 KEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRVI 491

Query: 421 TSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           TSA EA+KFTV NFIAG SWLPGTGVPFT GL
Sbjct: 492 TSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 519

[Close]

BLAST of Moc06g40380 vs. NCBI nr
Match: KAA0055086.1 (pectinesterase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 778.1 bits (2008), Expect = 4.2e-221
Identity = 380/452 (84.07%), Postives = 412/452 (91.15%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDKCSGFDIQTWLSS 60
           M+RA+  ETLTK LGQKCRN+KERAAWADC+EL+Q+TILHLNKT SD CS FDIQTWLSS
Sbjct: 1   MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHCSNFDIQTWLSS 60

Query: 61  ALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRGGF 120
           ALTNLDTCRAGF DL + DYG VFP LANNN+TKLISNSLAMNNCSES+   G     GF
Sbjct: 61  ALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESDE--GNTSDEGF 120

Query: 121 PTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGVYK 180
           P WVRGGDRRLLQAA PKAD+VVAQDGSGNY+T+ AA+ AA KR GSGRFVI +K+GVYK
Sbjct: 121 PKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYK 180

Query: 181 ENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 240
           EN+VI  K+KN+ML+GDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA
Sbjct: 181 ENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 240

Query: 241 GPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVL 300
           GPQNHQAVALRSG+DLSVFF+C FEGYQDTLY HSQRQFYRECYIYGT+DFIFGNAAVVL
Sbjct: 241 GPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVVL 300

Query: 301 QNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGRPW 360
           QNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV  SVRTYLGRPW
Sbjct: 301 QNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRPW 360

Query: 361 KEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYRVI 420
           KEYSRTVYL+++++ LVNPAGWLEWSGNFALNTLYYGE+ N+GPGSST+NRVKWKGYRVI
Sbjct: 361 KEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRVI 420

Query: 421 TSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           TSA EA+KFTV NFIAG SWLPGTGVPFT GL
Sbjct: 421 TSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 448

[Close]

BLAST of Moc06g40380 vs. NCBI nr
Match: XP_004143735.1 (pectinesterase 2 [Cucumis sativus] >KGN50327.1 hypothetical protein Csa_000416 [Cucumis sativus])

HSP 1 Score: 765.0 bits (1974), Expect = 3.6e-217
Identity = 377/453 (83.22%), Postives = 410/453 (90.51%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDK-CSGFDIQTWLS 60
           M+RA+  ETLTK LGQKCRN+KERAAWADC+EL+Q+TILHLNKT SDK CS FDIQTWLS
Sbjct: 72  MERAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDKNCSNFDIQTWLS 131

Query: 61  SALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRGG 120
           SALTNL TCRAGF DL + DYG VFP L NNN+TKLISNSLAMNNCSES+   G     G
Sbjct: 132 SALTNLHTCRAGFVDLGIKDYGVVFPFLENNNITKLISNSLAMNNCSESDE--GNTSDEG 191

Query: 121 FPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGVY 180
           FP W+ GGDRRLLQAA PKAD+VVAQDGSGNY+T+ AA+ AA KR GSGRFVI VK+GVY
Sbjct: 192 FPKWLHGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRVKKGVY 251

Query: 181 KENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNT 240
           KEN+VI  K+KN+ML+GDGL+YTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNT
Sbjct: 252 KENVVI--KVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNT 311

Query: 241 AGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVV 300
           AGPQNHQAVALRSG+DLSVFF+C FEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN+AVV
Sbjct: 312 AGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNSAVV 371

Query: 301 LQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGRP 360
           LQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA  DLKPV  SVRTYLGRP
Sbjct: 372 LQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMATDDLKPVIKSVRTYLGRP 431

Query: 361 WKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYRV 420
           WKEYSRTVYL++++D LVNPAGWLEWSGNFALNTLYYGE+ N GPGSST+NRVKWKGYRV
Sbjct: 432 WKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEFKNSGPGSSTANRVKWKGYRV 491

Query: 421 ITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           ITSA+EA+KFTV +FIAG SWLPGTGVPFT GL
Sbjct: 492 ITSASEAAKFTVGSFIAGNSWLPGTGVPFTAGL 520

[Close]

BLAST of Moc06g40380 vs. NCBI nr
Match: XP_022996028.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 372/454 (81.94%), Postives = 412/454 (90.75%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDK-CSGFDIQTWLS 60
           M+RAL AETLTK+  QKCR  KE+ AWADC+EL+Q TILHLN+T SD  CS FDIQTWLS
Sbjct: 68  MERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCSDFDIQTWLS 127

Query: 61  SALTNLDTCRAGFADLHVADYGAVFPLLA-NNNVTKLISNSLAMNNCSESESELGAGYRG 120
           SALTNL+TCRAGF DL++ DY  VFP L+ +NNVTKLI NSLAMNNCSE+E+    GY G
Sbjct: 128 SALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETET----GYHG 187

Query: 121 GFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGV 180
           GFPTWV+ G+RRLLQAA PKAD+VVA DGSGNY+T+ AA+ AA KR GSGRFVIHVK+GV
Sbjct: 188 GFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGV 247

Query: 181 YKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRN 240
           YKEN+VIG+K+KN+ML+GDGL+YTIITGSRSVGGGSTTFNSATVAVTGE FIARGITFRN
Sbjct: 248 YKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRN 307

Query: 241 TAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 300
           TAGP+NHQAVALRSGSDLSVF++CSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV
Sbjct: 308 TAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 367

Query: 301 VLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGR 360
           VLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PV SSVRTYLGR
Sbjct: 368 VLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTYLGR 427

Query: 361 PWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYR 420
           PW+EYSRTVYL+ ++D LVNPAGWLEWSG+FALNTLYYGE+ NFGPGSST+ RVKWKGYR
Sbjct: 428 PWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWKGYR 487

Query: 421 VITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           VITSA EASKFTV +FIAGKSWLP TGVPFT GL
Sbjct: 488 VITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

[Close]

BLAST of Moc06g40380 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.7e-172
Identity = 307/454 (67.62%), Postives = 368/454 (81.06%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKT--VSDKCSGFDIQTWL 60
           ++RA  A++ T  LG KCRN++E+AAW DC EL++ T+L LN+T   S  C+  D QTWL
Sbjct: 69  LERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWL 128

Query: 61  SSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRG 120
           S+ALTNL+TCRA   DL V +Y  V PLL +NNVTKLISN+L++N    +E      Y+ 
Sbjct: 129 STALTNLETCRASLEDLGVPEY--VLPLL-SNNVTKLISNTLSLNKVPYNE----PSYKD 188

Query: 121 GFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGV 180
           GFPTWV+ GDR+LLQ  +P+A++VVAQDGSGN +TI  A+AAA  R+G  R+VI++K G 
Sbjct: 189 GFPTWVKPGDRKLLQ-TTPRANIVVAQDGSGNVKTIQEAVAAA-SRAGGSRYVIYIKAGT 248

Query: 181 YKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRN 240
           Y EN+ +  KLKNIM +GDG+  TIITGS+SVGGG+TTF SATVAV G+ FIAR IT RN
Sbjct: 249 YNENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRN 308

Query: 241 TAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 300
           TAGP NHQAVALRSGSDLSVF++CSFEGYQDTLYVHSQRQFYREC IYGTVDFIFGNAAV
Sbjct: 309 TAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAV 368

Query: 301 VLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGR 360
           VLQNC I+ARKP P +   +TAQGRTDPNQ+TGI IHN RV AA+DLKPVQSSV+T+LGR
Sbjct: 369 VLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGR 428

Query: 361 PWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYR 420
           PWK+YSRTVY+K +LD L+NPAGW+EWSG+FALNTLYY EY N GPGSST+NRVKW+GY 
Sbjct: 429 PWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYH 488

Query: 421 VITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           V+TS ++ S+FTV NFIAG SWLP T VPFT GL
Sbjct: 489 VLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of Moc06g40380 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-158
Identity = 286/454 (63.00%), Postives = 356/454 (78.41%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSD--KCSGFDIQTWL 60
           +DRA++A+T    LG KCR+ +E+AAW DC++L+  T+  +N+T+    KCS  D QTWL
Sbjct: 71  LDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWL 130

Query: 61  SSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRG 120
           S+ALTNLDTCRAGF +L V D   V PL+ +NNV+ L+ N+LA+N    + +      + 
Sbjct: 131 STALTNLDTCRAGFLELGVTD--IVLPLM-SNNVSNLLCNTLAINKVPFNYT---PPEKD 190

Query: 121 GFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGV 180
           GFP+WV+ GDR+LLQ+++PK + VVA+DGSGN++TI  A+ AA   SGSGRFVI+VK+GV
Sbjct: 191 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQGV 250

Query: 181 YKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRN 240
           Y ENL I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+ FIARGITFRN
Sbjct: 251 YSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRN 310

Query: 241 TAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 300
           TAG  N QAVALRSGSDLSVF+QCSFE YQDTLYVHS RQFYR+C +YGTVDFIFGNAA 
Sbjct: 311 TAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAA 370

Query: 301 VLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGR 360
           VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV AA+DL+PV  S +TYLGR
Sbjct: 371 VLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGR 430

Query: 361 PWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYR 420
           PW++YSRTV++K  LD L++P GWLEW GNFAL TL+Y E+ N GPG+STS RV W G+R
Sbjct: 431 PWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFR 490

Query: 421 VITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           V+ SA+EASKFTV  F+AG SW+P + VPFT GL
Sbjct: 491 VLGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

[Close]

BLAST of Moc06g40380 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 5.6e-144
Identity = 260/466 (55.79%), Postives = 335/466 (71.89%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTV---------SDKCSG 60
           MDRA+ A       G+ C + K++A  ADC++L+  TI+ LN+T+         +  C+ 
Sbjct: 75  MDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTD 134

Query: 61  FDIQTWLSSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESE 120
           FD QTWLS+ALTN +TCR G +DL+V D+  + P+++N  ++ LISN LA+N    +   
Sbjct: 135 FDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVSNTKISHLISNCLAVNGALLTAGN 194

Query: 121 LG--AGYRGGFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRS-GSG 180
            G     + GFPTW+   D+RLL+A   +A++VVA+DGSG++ T+ AA+  A +R   SG
Sbjct: 195 KGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAIDVAGRRKVTSG 254

Query: 181 RFVIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGER 240
           RFVI+VKRG+Y+EN+ +     +IML+GDG+R TIITG RSV GG TT+NSAT  + G  
Sbjct: 255 RFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLH 314

Query: 241 FIARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGT 300
           FIA+GITFRNTAGP   QAVALRS SDLS+F++CS EGYQDTL VHSQRQFYRECYIYGT
Sbjct: 315 FIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGT 374

Query: 301 VDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPV 360
           VDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTGISIHNSR++ A DLKPV
Sbjct: 375 VDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLKPV 434

Query: 361 QSSVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEW--SGNFALNTLYYGEYNNFGPGS 420
             +V+TY+GRPW ++SRTV L+ YLD +V+P GW  W     F L+TL+Y EY N GP S
Sbjct: 435 VGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPAS 494

Query: 421 STSNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           ST  RV WKG+ V+  A++AS FTV  FIAG +WLP TG+PFT GL
Sbjct: 495 STRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of Moc06g40380 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 7.3e-144
Identity = 258/461 (55.97%), Postives = 336/461 (72.89%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSD-------KCSGFD 60
           MDRA+ A        + C + K++A  ADC+ L+  T++ LN+T+         +C+ FD
Sbjct: 79  MDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFD 138

Query: 61  IQTWLSSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNC--SESESE 120
            QTWLS+ALTN +TCR G +DL+V+D+    P+++N  ++ LISN LA+N    +  +++
Sbjct: 139 AQTWLSTALTNTETCRRGSSDLNVSDF--TTPIVSNTKISHLISNCLAVNGALLTAGKND 198

Query: 121 LGAGYRGGFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRS-GSGRF 180
              G   GFPTWV   +RRLLQ  S +A++VVA+DGSG+++T+ AA+  A +R   SGRF
Sbjct: 199 STTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRF 258

Query: 181 VIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFI 240
           VI+VKRG+Y+ENL +     NIML+GDG+RYTIITG RSV GG TT++SAT  + G  FI
Sbjct: 259 VIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGLHFI 318

Query: 241 ARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVD 300
           A+GI F+NTAGP   QAVALRS SDLS+F++CS EGYQDTL VHSQRQFYRECYIYGTVD
Sbjct: 319 AKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYGTVD 378

Query: 301 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQS 360
           FIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHNS ++ A DLKPV  
Sbjct: 379 FIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKPVVR 438

Query: 361 SVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEWS--GNFALNTLYYGEYNNFGPGSST 420
           SV+TY+GRPW  YSRTV LK Y+D +V+P GW  W+    + L+TL+Y EY N GP SST
Sbjct: 439 SVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPASST 498

Query: 421 SNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFT 450
             RV+WKG+ V++ A++AS F+V  FIAG +WLPG+G+PFT
Sbjct: 499 RWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFT 537

[Close]

BLAST of Moc06g40380 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.0e-141
Identity = 255/468 (54.49%), Postives = 336/468 (71.79%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTV--------SD-KCSG 60
           +D+A++  + T + G  C N + +AAW+DCV LFQ+T+  LN+T+        SD KC+ 
Sbjct: 129 LDQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTD 188

Query: 61  FDIQTWLSSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNN--CSESE 120
           FD QTWLS+A TN++TCR+G  DL+V+D+  V P+++N N++ LI N LA+N     + +
Sbjct: 189 FDAQTWLSTAQTNIETCRSGSEDLNVSDF--VMPVISNKNLSDLIGNCLAVNGVLMKQHD 248

Query: 121 SELGAGYRGGFPTWVRGGDRRLLQAAS---PKADVVVAQDGSGNYRTIAAALAAAEKRSG 180
               A ++  FP+WV   +RRLL +AS       +VVAQD SG++R+I AA+  A +R  
Sbjct: 249 HTTTANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRF 308

Query: 181 SGRFVIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTG 240
             RFVI+VK+GVY+EN+ +G+   NIML+GDG R TIIT  RSV  G TT+NSAT    G
Sbjct: 309 KSRFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGG 368

Query: 241 ERFIARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIY 300
           +RF+A+ +TF NTAGP   QAVA+RS SDLSVF++    G+QDTLY+HSQRQF+RECYI 
Sbjct: 369 QRFVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYIS 428

Query: 301 GTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLK 360
           GT+DFIFGNAAVV QNCMI  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++AA+DLK
Sbjct: 429 GTIDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLK 488

Query: 361 PVQSSVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEW--SGNFALNTLYYGEYNNFGP 420
           PV  + +TYLGRPW+ YSR   +K Y+D  ++P GW  W    NFALNT++YGEY NFGP
Sbjct: 489 PVIRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGP 548

Query: 421 GSSTSNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           GSST  RV+WKG+  ITS   AS+FTV + IAG SWLP TGVPF  GL
Sbjct: 549 GSSTRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Moc06g40380 vs. ExPASy TrEMBL
Match: A0A5A7ULB7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.0e-221
Identity = 380/452 (84.07%), Postives = 412/452 (91.15%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDKCSGFDIQTWLSS 60
           M+RA+  ETLTK LGQKCRN+KERAAWADC+EL+Q+TILHLNKT SD CS FDIQTWLSS
Sbjct: 1   MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHCSNFDIQTWLSS 60

Query: 61  ALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRGGF 120
           ALTNLDTCRAGF DL + DYG VFP LANNN+TKLISNSLAMNNCSES+   G     GF
Sbjct: 61  ALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESDE--GNTSDEGF 120

Query: 121 PTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGVYK 180
           P WVRGGDRRLLQAA PKAD+VVAQDGSGNY+T+ AA+ AA KR GSGRFVI +K+GVYK
Sbjct: 121 PKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYK 180

Query: 181 ENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 240
           EN+VI  K+KN+ML+GDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA
Sbjct: 181 ENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 240

Query: 241 GPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVL 300
           GPQNHQAVALRSG+DLSVFF+C FEGYQDTLY HSQRQFYRECYIYGT+DFIFGNAAVVL
Sbjct: 241 GPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVVL 300

Query: 301 QNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGRPW 360
           QNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV  SVRTYLGRPW
Sbjct: 301 QNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRPW 360

Query: 361 KEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYRVI 420
           KEYSRTVYL+++++ LVNPAGWLEWSGNFALNTLYYGE+ N+GPGSST+NRVKWKGYRVI
Sbjct: 361 KEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRVI 420

Query: 421 TSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           TSA EA+KFTV NFIAG SWLPGTGVPFT GL
Sbjct: 421 TSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 448

[Close]

BLAST of Moc06g40380 vs. ExPASy TrEMBL
Match: A0A1S3ASJ4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.0e-221
Identity = 380/452 (84.07%), Postives = 412/452 (91.15%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDKCSGFDIQTWLSS 60
           M+RA+  ETLTK LGQKCRN+KERAAWADC+EL+Q+TILHLNKT SD CS FDIQTWLSS
Sbjct: 72  MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHCSNFDIQTWLSS 131

Query: 61  ALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRGGF 120
           ALTNLDTCRAGF DL + DYG VFP LANNN+TKLISNSLAMNNCSES+   G     GF
Sbjct: 132 ALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESDE--GNTSDEGF 191

Query: 121 PTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGVYK 180
           P WVRGGDRRLLQAA PKAD+VVAQDGSGNY+T+ AA+ AA KR GSGRFVI +K+GVYK
Sbjct: 192 PKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYK 251

Query: 181 ENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 240
           EN+VI  K+KN+ML+GDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA
Sbjct: 252 ENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTA 311

Query: 241 GPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVL 300
           GPQNHQAVALRSG+DLSVFF+C FEGYQDTLY HSQRQFYRECYIYGT+DFIFGNAAVVL
Sbjct: 312 GPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVVL 371

Query: 301 QNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGRPW 360
           QNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV  SVRTYLGRPW
Sbjct: 372 QNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRPW 431

Query: 361 KEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYRVI 420
           KEYSRTVYL+++++ LVNPAGWLEWSGNFALNTLYYGE+ N+GPGSST+NRVKWKGYRVI
Sbjct: 432 KEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRVI 491

Query: 421 TSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           TSA EA+KFTV NFIAG SWLPGTGVPFT GL
Sbjct: 492 TSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 519

[Close]

BLAST of Moc06g40380 vs. ExPASy TrEMBL
Match: A0A0A0KPG9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.8e-217
Identity = 377/453 (83.22%), Postives = 410/453 (90.51%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDK-CSGFDIQTWLS 60
           M+RA+  ETLTK LGQKCRN+KERAAWADC+EL+Q+TILHLNKT SDK CS FDIQTWLS
Sbjct: 72  MERAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDKNCSNFDIQTWLS 131

Query: 61  SALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRGG 120
           SALTNL TCRAGF DL + DYG VFP L NNN+TKLISNSLAMNNCSES+   G     G
Sbjct: 132 SALTNLHTCRAGFVDLGIKDYGVVFPFLENNNITKLISNSLAMNNCSESDE--GNTSDEG 191

Query: 121 FPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGVY 180
           FP W+ GGDRRLLQAA PKAD+VVAQDGSGNY+T+ AA+ AA KR GSGRFVI VK+GVY
Sbjct: 192 FPKWLHGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRVKKGVY 251

Query: 181 KENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNT 240
           KEN+VI  K+KN+ML+GDGL+YTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNT
Sbjct: 252 KENVVI--KVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNT 311

Query: 241 AGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVV 300
           AGPQNHQAVALRSG+DLSVFF+C FEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN+AVV
Sbjct: 312 AGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNSAVV 371

Query: 301 LQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGRP 360
           LQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA  DLKPV  SVRTYLGRP
Sbjct: 372 LQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMATDDLKPVIKSVRTYLGRP 431

Query: 361 WKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYRV 420
           WKEYSRTVYL++++D LVNPAGWLEWSGNFALNTLYYGE+ N GPGSST+NRVKWKGYRV
Sbjct: 432 WKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEFKNSGPGSSTANRVKWKGYRV 491

Query: 421 ITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           ITSA+EA+KFTV +FIAG SWLPGTGVPFT GL
Sbjct: 492 ITSASEAAKFTVGSFIAGNSWLPGTGVPFTAGL 520

[Close]

BLAST of Moc06g40380 vs. ExPASy TrEMBL
Match: A0A6J1K9P3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.7e-216
Identity = 372/454 (81.94%), Postives = 412/454 (90.75%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDK-CSGFDIQTWLS 60
           M+RAL AETLTK+  QKCR  KE+ AWADC+EL+Q TILHLN+T SD  CS FDIQTWLS
Sbjct: 68  MERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCSDFDIQTWLS 127

Query: 61  SALTNLDTCRAGFADLHVADYGAVFPLLA-NNNVTKLISNSLAMNNCSESESELGAGYRG 120
           SALTNL+TCRAGF DL++ DY  VFP L+ +NNVTKLI NSLAMNNCSE+E+    GY G
Sbjct: 128 SALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETET----GYHG 187

Query: 121 GFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGV 180
           GFPTWV+ G+RRLLQAA PKAD+VVA DGSGNY+T+ AA+ AA KR GSGRFVIHVK+GV
Sbjct: 188 GFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGV 247

Query: 181 YKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRN 240
           YKEN+VIG+K+KN+ML+GDGL+YTIITGSRSVGGGSTTFNSATVAVTGE FIARGITFRN
Sbjct: 248 YKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRN 307

Query: 241 TAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 300
           TAGP+NHQAVALRSGSDLSVF++CSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV
Sbjct: 308 TAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 367

Query: 301 VLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGR 360
           VLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PV SSVRTYLGR
Sbjct: 368 VLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTYLGR 427

Query: 361 PWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYR 420
           PW+EYSRTVYL+ ++D LVNPAGWLEWSG+FALNTLYYGE+ NFGPGSST+ RVKWKGYR
Sbjct: 428 PWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWKGYR 487

Query: 421 VITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           VITSA EASKFTV +FIAGKSWLP TGVPFT GL
Sbjct: 488 VITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

[Close]

BLAST of Moc06g40380 vs. ExPASy TrEMBL
Match: A0A6J1H1Z9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.4e-213
Identity = 368/454 (81.06%), Postives = 408/454 (89.87%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSDK-CSGFDIQTWLS 60
           M+RAL AE+LTK+  QKCR  KE+ AWADC+EL+Q TIL LN+T SD  CS FDIQTWLS
Sbjct: 68  MERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLS 127

Query: 61  SALTNLDTCRAGFADLHVADYGAVFPLLA-NNNVTKLISNSLAMNNCSESESELGAGYRG 120
           SALTNL+TCRAGF DL++ DY  VFP L  +NNVTKLI NSLAMNNCSE+E+    GY G
Sbjct: 128 SALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETET----GYHG 187

Query: 121 GFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGV 180
           GFPTWV+ G+RRLLQAA PKAD+VVA DGSGNY+T+ AA+ AA KR GSGRFVIHVK+GV
Sbjct: 188 GFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGV 247

Query: 181 YKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRN 240
           YKEN+VIG+K+KN+ML+GDGL+YTIITGSRSV GGSTTFNSATVAVTGE FIARGITFRN
Sbjct: 248 YKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGFIARGITFRN 307

Query: 241 TAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 300
           TAGPQNHQAVALRSGSDLSVF++C FEGYQDTLYVHSQRQFY+ECYIYGTVDFIFGNAAV
Sbjct: 308 TAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAV 367

Query: 301 VLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGR 360
           VLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PV SSVRTYLGR
Sbjct: 368 VLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTYLGR 427

Query: 361 PWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYR 420
           PWKEYSRTVY++ ++D LVNPAGWLEWSG+FALNTLYYGE+ NFGPGSST+ RVKWKGYR
Sbjct: 428 PWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWKGYR 487

Query: 421 VITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           VITSA EASKFTV +FIAGKSWLP TGVPFT GL
Sbjct: 488 VITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

[Close]

BLAST of Moc06g40380 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 561.2 bits (1445), Expect = 7.4e-160
Identity = 286/454 (63.00%), Postives = 356/454 (78.41%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSD--KCSGFDIQTWL 60
           +DRA++A+T    LG KCR+ +E+AAW DC++L+  T+  +N+T+    KCS  D QTWL
Sbjct: 71  LDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWL 130

Query: 61  SSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESELGAGYRG 120
           S+ALTNLDTCRAGF +L V D   V PL+ +NNV+ L+ N+LA+N    + +      + 
Sbjct: 131 STALTNLDTCRAGFLELGVTD--IVLPLM-SNNVSNLLCNTLAINKVPFNYT---PPEKD 190

Query: 121 GFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRSGSGRFVIHVKRGV 180
           GFP+WV+ GDR+LLQ+++PK + VVA+DGSGN++TI  A+ AA   SGSGRFVI+VK+GV
Sbjct: 191 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQGV 250

Query: 181 YKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRN 240
           Y ENL I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+ FIARGITFRN
Sbjct: 251 YSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRN 310

Query: 241 TAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAV 300
           TAG  N QAVALRSGSDLSVF+QCSFE YQDTLYVHS RQFYR+C +YGTVDFIFGNAA 
Sbjct: 311 TAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAA 370

Query: 301 VLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQSSVRTYLGR 360
           VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV AA+DL+PV  S +TYLGR
Sbjct: 371 VLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGR 430

Query: 361 PWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYNNFGPGSSTSNRVKWKGYR 420
           PW++YSRTV++K  LD L++P GWLEW GNFAL TL+Y E+ N GPG+STS RV W G+R
Sbjct: 431 PWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFR 490

Query: 421 VITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           V+ SA+EASKFTV  F+AG SW+P + VPFT GL
Sbjct: 491 VLGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

[Close]

BLAST of Moc06g40380 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 512.3 bits (1318), Expect = 4.0e-145
Identity = 260/466 (55.79%), Postives = 335/466 (71.89%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTV---------SDKCSG 60
           MDRA+ A       G+ C + K++A  ADC++L+  TI+ LN+T+         +  C+ 
Sbjct: 75  MDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTD 134

Query: 61  FDIQTWLSSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNCSESESE 120
           FD QTWLS+ALTN +TCR G +DL+V D+  + P+++N  ++ LISN LA+N    +   
Sbjct: 135 FDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVSNTKISHLISNCLAVNGALLTAGN 194

Query: 121 LG--AGYRGGFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRS-GSG 180
            G     + GFPTW+   D+RLL+A   +A++VVA+DGSG++ T+ AA+  A +R   SG
Sbjct: 195 KGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAIDVAGRRKVTSG 254

Query: 181 RFVIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGER 240
           RFVI+VKRG+Y+EN+ +     +IML+GDG+R TIITG RSV GG TT+NSAT  + G  
Sbjct: 255 RFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLH 314

Query: 241 FIARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGT 300
           FIA+GITFRNTAGP   QAVALRS SDLS+F++CS EGYQDTL VHSQRQFYRECYIYGT
Sbjct: 315 FIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGT 374

Query: 301 VDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPV 360
           VDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTGISIHNSR++ A DLKPV
Sbjct: 375 VDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLKPV 434

Query: 361 QSSVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEW--SGNFALNTLYYGEYNNFGPGS 420
             +V+TY+GRPW ++SRTV L+ YLD +V+P GW  W     F L+TL+Y EY N GP S
Sbjct: 435 VGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPAS 494

Query: 421 STSNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           ST  RV WKG+ V+  A++AS FTV  FIAG +WLP TG+PFT GL
Sbjct: 495 STRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of Moc06g40380 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 511.9 bits (1317), Expect = 5.2e-145
Identity = 258/461 (55.97%), Postives = 336/461 (72.89%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTVSD-------KCSGFD 60
           MDRA+ A        + C + K++A  ADC+ L+  T++ LN+T+         +C+ FD
Sbjct: 79  MDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFD 138

Query: 61  IQTWLSSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNNC--SESESE 120
            QTWLS+ALTN +TCR G +DL+V+D+    P+++N  ++ LISN LA+N    +  +++
Sbjct: 139 AQTWLSTALTNTETCRRGSSDLNVSDF--TTPIVSNTKISHLISNCLAVNGALLTAGKND 198

Query: 121 LGAGYRGGFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAAAEKRS-GSGRF 180
              G   GFPTWV   +RRLLQ  S +A++VVA+DGSG+++T+ AA+  A +R   SGRF
Sbjct: 199 STTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRF 258

Query: 181 VIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFI 240
           VI+VKRG+Y+ENL +     NIML+GDG+RYTIITG RSV GG TT++SAT  + G  FI
Sbjct: 259 VIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGLHFI 318

Query: 241 ARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIYGTVD 300
           A+GI F+NTAGP   QAVALRS SDLS+F++CS EGYQDTL VHSQRQFYRECYIYGTVD
Sbjct: 319 AKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYGTVD 378

Query: 301 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVQS 360
           FIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHNS ++ A DLKPV  
Sbjct: 379 FIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKPVVR 438

Query: 361 SVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEWS--GNFALNTLYYGEYNNFGPGSST 420
           SV+TY+GRPW  YSRTV LK Y+D +V+P GW  W+    + L+TL+Y EY N GP SST
Sbjct: 439 SVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPASST 498

Query: 421 SNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFT 450
             RV+WKG+ V++ A++AS F+V  FIAG +WLPG+G+PFT
Sbjct: 499 RWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFT 537

[Close]

BLAST of Moc06g40380 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 503.8 bits (1296), Expect = 1.4e-142
Identity = 255/468 (54.49%), Postives = 336/468 (71.79%), Query Frame = 0


Query: 1   MDRALVAETLTKQLGQKCRNQKERAAWADCVELFQSTILHLNKTV--------SD-KCSG 60
           +D+A++  + T + G  C N + +AAW+DCV LFQ+T+  LN+T+        SD KC+ 
Sbjct: 129 LDQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTD 188

Query: 61  FDIQTWLSSALTNLDTCRAGFADLHVADYGAVFPLLANNNVTKLISNSLAMNN--CSESE 120
           FD QTWLS+A TN++TCR+G  DL+V+D+  V P+++N N++ LI N LA+N     + +
Sbjct: 189 FDAQTWLSTAQTNIETCRSGSEDLNVSDF--VMPVISNKNLSDLIGNCLAVNGVLMKQHD 248

Query: 121 SELGAGYRGGFPTWVRGGDRRLLQAAS---PKADVVVAQDGSGNYRTIAAALAAAEKRSG 180
               A ++  FP+WV   +RRLL +AS       +VVAQD SG++R+I AA+  A +R  
Sbjct: 249 HTTTANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRF 308

Query: 181 SGRFVIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSATVAVTG 240
             RFVI+VK+GVY+EN+ +G+   NIML+GDG R TIIT  RSV  G TT+NSAT    G
Sbjct: 309 KSRFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGG 368

Query: 241 ERFIARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFYRECYIY 300
           +RF+A+ +TF NTAGP   QAVA+RS SDLSVF++    G+QDTLY+HSQRQF+RECYI 
Sbjct: 369 QRFVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYIS 428

Query: 301 GTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLK 360
           GT+DFIFGNAAVV QNCMI  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++AA+DLK
Sbjct: 429 GTIDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLK 488

Query: 361 PVQSSVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEW--SGNFALNTLYYGEYNNFGP 420
           PV  + +TYLGRPW+ YSR   +K Y+D  ++P GW  W    NFALNT++YGEY NFGP
Sbjct: 489 PVIRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGP 548

Query: 421 GSSTSNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           GSST  RV+WKG+  ITS   AS+FTV + IAG SWLP TGVPF  GL
Sbjct: 549 GSSTRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Moc06g40380 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 442.6 bits (1137), Expect = 3.9e-124
Identity = 240/472 (50.85%), Postives = 304/472 (64.41%), Query Frame = 0


Query: 22  KERAAWADCVELFQSTILHLNKTVSD----------KCSGFDIQTWLSSALTNLDTCRAG 81
           +E+ A  DC+E    T+  L++TV D          +    D++T +SSA+TN +TC  G
Sbjct: 122 REKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDG 181

Query: 82  FADLHVADYGAVFPLLANNNVTKLISNSLAM--------------------NNCSESESE 141
           F+            L    +V  + SN+LAM                    NN    E  
Sbjct: 182 FSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEEN 241

Query: 142 ---------LGAGY--RGGFPTWVRGGDRRLLQAASPKADVVVAQDGSGNYRTIAAALAA 201
                     GAG     G+PTW+  GDRRLLQ +  KAD  VA DGSG ++T+AAA+AA
Sbjct: 242 QETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAA 301

Query: 202 AEKRSGSGRFVIHVKRGVYKENLVIGSKLKNIMLLGDGLRYTIITGSRSVGGGSTTFNSA 261
           A + S   R+VIH+K GVY+EN+ +  K KNIM +GDG   TIITGSR+V  GSTTF+SA
Sbjct: 302 APENSNK-RYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSA 361

Query: 262 TVAVTGERFIARGITFRNTAGPQNHQAVALRSGSDLSVFFQCSFEGYQDTLYVHSQRQFY 321
           TVA  GERF+AR ITF+NTAGP  HQAVALR GSD S F+ C    YQDTLYVHS RQF+
Sbjct: 362 TVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFF 421

Query: 322 RECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVM 381
            +C I GTVDFIFGNAAVVLQ+C I+AR+P  GQ+ +VTAQGRTDPNQNTGI I   R+ 
Sbjct: 422 VKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIG 481

Query: 382 AAADLKPVQSSVRTYLGRPWKEYSRTVYLKNYLDGLVNPAGWLEWSGNFALNTLYYGEYN 441
           A +DL+ V+ S  TYLGRPWKEYS+TV +++ +  ++ P GW EW+G FALNTL Y EY+
Sbjct: 482 ATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYS 541

Query: 442 NFGPGSSTSNRVKWKGYRVITSATEASKFTVENFIAGKSWLPGTGVPFTVGL 453
           N G G+ T+NRVKW+G++VIT+A EA K+T   FI G  WL  TG PF++GL
Sbjct: 542 NTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

The following BLAST results are available for this feature:

BLAST of Moc06g40380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875884.1	4.0e-224	85.71	pectinesterase 2-like [Benincasa hispida]	[more]
XP_008436965.1	4.2e-221	84.07	PREDICTED: pectinesterase 2-like [Cucumis melo]	[more]
KAA0055086.1	4.2e-221	84.07	pectinesterase 2-like [Cucumis melo var. makuwa]	[more]
XP_004143735.1	3.6e-217	83.22	pectinesterase 2 [Cucumis sativus] >KGN50327.1 hypothetical protein Csa_000416 [...	[more]
XP_022996028.1	1.2e-215	81.94	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of Moc06g40380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	3.7e-172	67.62	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	1.0e-158	63.00	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	5.6e-144	55.79	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	7.3e-144	55.97	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	2.0e-141	54.49	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Moc06g40380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7ULB7	2.0e-221	84.07	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120...	[more]
A0A1S3ASJ4	2.0e-221	84.07	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1	[more]
A0A0A0KPG9	1.8e-217	83.22	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1	[more]
A0A6J1K9P3	5.7e-216	81.94	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1	[more]
A0A6J1H1Z9	3.4e-213	81.06	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1	[more]

BLAST of Moc06g40380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	7.4e-160	63.00	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	4.0e-145	55.79	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	5.2e-145	55.97	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	1.4e-142	54.49	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G14310.1	3.9e-124	50.85	pectin methylesterase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 1..102 e-value: 4.6E-7 score: 39.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 9..104 e-value: 3.1E-13 score: 47.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 6..102 e-value: 1.2E-16 score: 61.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 134..452 e-value: 7.8E-126 score: 421.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 1..105 e-value: 2.8E-16 score: 61.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 4..105
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 140..437 e-value: 1.0E-145 score: 484.1
None	No IPR available	PANTHER	PTHR31707:SF181	PECTINESTERASE	coord: 1..451
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 1..451
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 1..104 e-value: 2.19287E-24 score: 96.7384
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 285..294
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 166..185
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 136..451

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g40380.1	Moc06g40380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity