Moc06g39670 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc06g39670 vs. NCBI nr
Match: XP_022159652.1 (subtilisin-like protease SBT1.7 [Momordica charantia])

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 748/750 (99.73%), Postives = 748/750 (99.73%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSS 60
           MVVLPSLFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSS
Sbjct: 1   MVVLPSLFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSS 60

Query: 61  SEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNR 120
           SEDHPKLLYSF NVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNR
Sbjct: 61  SEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNR 120

Query: 121 QFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKG IIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLA 240
           RSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLA
Sbjct: 181 RSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKG 300
           HLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKG
Sbjct: 241 HLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKG 300

Query: 301 IFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFP 360
           IFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFP
Sbjct: 301 IFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFP 360

Query: 361 PTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAM 420
           PTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAM
Sbjct: 361 PTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAM 420

Query: 421 ILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSP 480
           ILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSP
Sbjct: 421 ILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSP 480

Query: 481 AIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPH 540
           AIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPH
Sbjct: 481 AIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPH 540

Query: 541 LSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSK 600
           LSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSK
Sbjct: 541 LSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSK 600

Query: 601 ATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG 660
           ATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG
Sbjct: 601 ATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG 660

Query: 661 PPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSI 720
           PPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSI
Sbjct: 661 PPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSI 720

Query: 721 TPPSSGSAQAYLKCMSSKHIVRSPISIKFV 751
           TPPSSGSAQAYLKCMSSKHIVRSPISIKFV
Sbjct: 721 TPPSSGSAQAYLKCMSSKHIVRSPISIKFV 750

BLAST of Moc06g39670 vs. NCBI nr
Match: XP_023532974.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1222.2 bits (3161), Expect = 0.0e+00
Identity = 622/752 (82.71%), Postives = 669/752 (88.96%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV  PSLFLL+L +FH  GA  T     NLQTYIVHV+KPE        +DLE+WHRSFL
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVEKPE------TTDDLESWHRSFL 60

Query: 61  PSSSEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 120
           PSSS     LLYS+RNVMSGFAARL+EE VKAMEEKDGF+SARRERIL+LHTTHTP+FLG
Sbjct: 61  PSSS----SLLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLG 120

Query: 121 LNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKL 180
           LNRQFGFWKDSNFGKG IIGVLDGGI P+HPSFDD GMPPPP KWKGRCEFNFSACNNKL
Sbjct: 121 LNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKL 180

Query: 181 IGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMA 240
           IGARSFNLATK LKGE     DSPIDEDGHGTHTASTAAGAFV  AEALGNAKGTAVGMA
Sbjct: 181 IGARSFNLATKVLKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMA 240

Query: 241 PLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAAT 300
           PLAHLAIYKVCFGEDCPD+DILAALDAA+EDGVDVLSLSLGS S PFF+D +AIGAFAA 
Sbjct: 241 PLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAI 300

Query: 301 QKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPS 360
           QKGIFVSCSA+NSGP  ATLSNEAPWILTVAASTIDRRI A AKLGNGEEFDGESLFQPS
Sbjct: 301 QKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPS 360

Query: 361 DFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGG 420
           DFPPT FLPLVYAGEKN+TAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EVKNAGG
Sbjct: 361 DFPPT-FLPLVYAGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGG 420

Query: 421 AAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDD 480
           AAMILLNQQ DGF+TEADAHVLPA+HV+H AALKIK YINST  P A I F+GTVIGDD+
Sbjct: 421 AAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDN 480

Query: 481 FSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMS 540
           FSPAIASFSSRGPS+ASPGILKPDITGPG+SILAAWPFPLD N+ NTK TFN+ISGTSMS
Sbjct: 481 FSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNS-NTKSTFNIISGTSMS 540

Query: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVN 600
           CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITN +G+PI+D+N QPADLFATGAGHVN
Sbjct: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVN 600

Query: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSV 660
           PSKA DPGLVYDIQPDDYIPYLCGLGYK NEV+ IA +P++C AKPSIPEG+LNYPSF+V
Sbjct: 601 PSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTV 660

Query: 661 ALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRI 720
            LGPPQTFTRTVTNVG G EVYT ++EAPPS +SVT+RP KIFFS +N+KV YSVTFKRI
Sbjct: 661 VLGPPQTFTRTVTNVGCGREVYTAVVEAPPS-ISVTIRPSKIFFSKINEKVTYSVTFKRI 720

Query: 721 GSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 750
           GSI+ PS+   + YLK +S KH+VRSPIS KF
Sbjct: 721 GSIS-PSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Moc06g39670 vs. NCBI nr
Match: XP_022958542.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata])

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 623/752 (82.85%), Postives = 668/752 (88.83%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV  PSLFLL+L +FH  GA  T     NLQTYIVHVKKPE        +DLE WHRSFL
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPE------TTDDLEIWHRSFL 60

Query: 61  PSSSEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 120
           PSSS     LLYS+RNVMSGFAARL+EE VKAMEE DGF+SARRERIL+LHTTHTP+FLG
Sbjct: 61  PSSS----SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLG 120

Query: 121 LNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKL 180
           LNRQFGFWKDSNFGKG IIGVLDGGI P+HPSFDD GMPPPP KWKGRCEFNFSACNNKL
Sbjct: 121 LNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKL 180

Query: 181 IGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMA 240
           IGARSFNLATKALKGE     DSPIDEDGHGTHTASTAAGAFV  AEALGNAKGTAVGMA
Sbjct: 181 IGARSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMA 240

Query: 241 PLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAAT 300
           PLAHLAIYKVCFGEDCPD+DILAALDAA+EDGVDVLSLSLGS S PFF+D +AIGAFAA 
Sbjct: 241 PLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAI 300

Query: 301 QKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPS 360
           QKGIFVSCSA+NSGP  ATLSNEAPWILTVAASTIDRRI A AKLGNGEEFDGESLFQPS
Sbjct: 301 QKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPS 360

Query: 361 DFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGG 420
           DFPPTL LPLVYAGEKN+TAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EVKNAGG
Sbjct: 361 DFPPTL-LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGG 420

Query: 421 AAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDD 480
           AAMILLNQQ DGF+TEADAHVLPA+HV+H AALKIK YINST  P A I F+GTVIGDD+
Sbjct: 421 AAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDN 480

Query: 481 FSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMS 540
           FSPAIASFSSRGPS+ASPGILKPDITGPG+SILAAWPFPLD N +NTK TFN+ISGTSMS
Sbjct: 481 FSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKN-RNTKSTFNIISGTSMS 540

Query: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVN 600
           CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITN +G+PI+D+N QPADLFATGAGHVN
Sbjct: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVN 600

Query: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSV 660
           PSKA DPGLVYDIQPDDYIPYLCGLGYK NEV+ IA +P++C AKPSIPEG+LNYPSF+V
Sbjct: 601 PSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTV 660

Query: 661 ALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRI 720
            LGPPQTFTRTVTNVG G EVYT ++EAPPS +SVT+RP KIFFS +NQKV +SVTFKRI
Sbjct: 661 VLGPPQTFTRTVTNVGCGREVYTAVVEAPPS-ISVTIRPSKIFFSKINQKVTFSVTFKRI 720

Query: 721 GSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 750
           GSI+ PS+   + YLK +S KH+VRSPIS KF
Sbjct: 721 GSIS-PSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Moc06g39670 vs. NCBI nr
Match: KAG7036083.1 (Subtilisin-like protease SBT1.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 622/752 (82.71%), Postives = 667/752 (88.70%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV  PSLFLL+L +FH  GA  T     NLQTYIVHVKKPE        +DLE WHRSFL
Sbjct: 65  MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPE------TTDDLEIWHRSFL 124

Query: 61  PSSSEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 120
           PSSS     LLYS+RNVMSGFAARL+EE VKAMEE DGF+SARRERIL+LHTTHTP+FLG
Sbjct: 125 PSSS----SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLG 184

Query: 121 LNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKL 180
           LNRQFGFWKDSNFGKG IIGVLDGGI P+HPSFDD GMPPPP KWKGRCEFNFSACNNKL
Sbjct: 185 LNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKL 244

Query: 181 IGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMA 240
           IGARSFNLATKALKGE     DSPIDEDGHGTHTASTAAGAFV  AEALGNAKGTAVGMA
Sbjct: 245 IGARSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMA 304

Query: 241 PLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAAT 300
           PLAHLAIYKVCFGEDCPD+DILAALDAA+EDGVDVLSLSLGS S PFF+D +AIGAFAA 
Sbjct: 305 PLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAI 364

Query: 301 QKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPS 360
           QKGIFVSCSA+NSGP  ATLSNEAPWILTVAASTIDRRI A AKLGNGEEFDGESLFQPS
Sbjct: 365 QKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPS 424

Query: 361 DFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGG 420
           DFPPTL LPLVYAGEKN+TAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EVKNAGG
Sbjct: 425 DFPPTL-LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGG 484

Query: 421 AAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDD 480
           AAMILLNQQ DGF+TEADAHVLPA+HV+H AALKIK YINST  P A I F+GTVIGDD+
Sbjct: 485 AAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDN 544

Query: 481 FSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMS 540
           FSPAIASFSSRGPS+ASPGILKPDITGPG+SILAAWPFPLD N +NTK TFN+ISGTSMS
Sbjct: 545 FSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKN-RNTKSTFNIISGTSMS 604

Query: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVN 600
           CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITN +G+PI+D+N QPADLFATGAGHVN
Sbjct: 605 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVN 664

Query: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSV 660
           PSKA DPGLVYDIQPDDYIPYLCGLGYK NEV+ IA +P++C AKPSIPEG+LNYPSF+V
Sbjct: 665 PSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTV 724

Query: 661 ALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRI 720
            LGPPQTFTRTVTNVG G EVYT ++EA PS +SVT+RP KIFFS +NQKV +SVTFKRI
Sbjct: 725 VLGPPQTFTRTVTNVGCGREVYTAVVEASPS-ISVTIRPSKIFFSKINQKVTFSVTFKRI 784

Query: 721 GSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 750
           GSI+ PS+   + YLK +S KH+VRSPIS KF
Sbjct: 785 GSIS-PSTEFGKGYLKWVSDKHVVRSPISFKF 801

BLAST of Moc06g39670 vs. NCBI nr
Match: KAG6606138.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 621/752 (82.58%), Postives = 666/752 (88.56%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV  PSLFLL+L  FH  GA  T     NLQTYIVHVKKPE        +DLE+WHRSFL
Sbjct: 1   MVAFPSLFLLLLLDFHYVGALVTELPLINLQTYIVHVKKPE------TTDDLESWHRSFL 60

Query: 61  PSSSEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 120
           PSSS     LLYS+RNVMSGFAARL+EE VKAMEE DGF+SARRERIL+LHTTHTP+FLG
Sbjct: 61  PSSS----SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLG 120

Query: 121 LNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKL 180
           LNRQFGFWKDSNFGKG IIGVLDGGI P+HPSFDD GMPPPP KWKGRCEFNFSACNNKL
Sbjct: 121 LNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKL 180

Query: 181 IGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMA 240
           IGARSFNLATKALKGE     DSPIDEDGHGTHTASTAAGAFV  AEALGNAKGTAVGMA
Sbjct: 181 IGARSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMA 240

Query: 241 PLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAAT 300
           PLAHLAIYKVCFGEDCPD+DILAALDAA+EDGVDVLSLSLGS S PFF+D +AIGAFAA 
Sbjct: 241 PLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAI 300

Query: 301 QKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPS 360
           QKGIFVSCSA+NSGP  ATLSNEAPWILTVAASTIDRRI A AKLGNGEE DGESLFQPS
Sbjct: 301 QKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEECDGESLFQPS 360

Query: 361 DFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGG 420
           DFPPTL LPLVYAGEKN+TAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EVKNAGG
Sbjct: 361 DFPPTL-LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGG 420

Query: 421 AAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDD 480
           AAMILLNQQ DGF+TEADAHVLPA+HV+H AALKIK YINST  P A I F+GTVIGDD+
Sbjct: 421 AAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDN 480

Query: 481 FSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMS 540
           FSPAIASFSSRGPS+ASPGILKPDITGPG+SILAAWPFPLD N +NTK TFN+ISGTSMS
Sbjct: 481 FSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKN-RNTKSTFNIISGTSMS 540

Query: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVN 600
           CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITN +G+PI+D+N QPADLFATGAGHVN
Sbjct: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVN 600

Query: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSV 660
           PSKA DPGLVYDIQPDDYIPYLCGLGYK NEV+ IA +P++C AKPSIPEG+LNYPSF+V
Sbjct: 601 PSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTV 660

Query: 661 ALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRI 720
            LGPPQTFTRTVTNVG G EVYT ++EA PS +SVT+RP KIFFS +NQKV +SVTFKRI
Sbjct: 661 VLGPPQTFTRTVTNVGCGREVYTAVVEASPS-ISVTIRPSKIFFSKINQKVTFSVTFKRI 720

Query: 721 GSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 750
           GSI+ PS+   + YLK +S KH+VRSPIS KF
Sbjct: 721 GSIS-PSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Moc06g39670 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 3.6e-281
Identity = 500/760 (65.79%), Postives = 595/760 (78.29%), Query Frame = 0

Query: 6   SLFLLVLFHFHG--GGARPT-------SSNLQTYIVHVKKPE-KLSTYGQVEDLENWHRS 65
           +L  L+ FH H   G   PT       +S+ + YI+HV  PE K+ T  + EDLE+W+ S
Sbjct: 8   ALTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLT--ESEDLESWYHS 67

Query: 66  FLP---SSSEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHT 125
           FLP    SSE+ P+++YS++NV+ GFAA LT+E + A+E+K+GFISA  +R+L   TTHT
Sbjct: 68  FLPPTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHT 127

Query: 126 PNFLGLNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSA 185
           P FLGL +  G WK+SNFGKG IIGVLD GITP HPSF D G+PPPP KWKGRC+ N +A
Sbjct: 128 PKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTA 187

Query: 186 CNNKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGT 245
           CNNKLIGAR+FNLA +A+ G++    ++PIDEDGHGTHTASTAAGAFV+ AE LGNAKGT
Sbjct: 188 CNNKLIGARAFNLAAEAMNGKK---AEAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGT 247

Query: 246 AVGMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLG-SDSAPFFRDDIAI 305
           A GMAP AHLAIYKVCFGEDCP+SDILAALDAAVEDGVDV+S+SLG S+  PFF D  AI
Sbjct: 248 AAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAI 307

Query: 306 GAFAATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGE 365
           GAFAA QKGIFVSC+A NSGP N+++ N APWILTV ASTIDRRI+ATAKLGNG+EFDGE
Sbjct: 308 GAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGE 367

Query: 366 SLFQPSDFPPTLFLPLVYAGEK-NETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGM 425
           S+FQPS F PTL LPL YAG+   E +A C  GSL D   +GK V+CERGGGIARIAKG 
Sbjct: 368 SVFQPSSFTPTL-LPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGE 427

Query: 426 EVKNAGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRG 485
           EVK AGGAAMIL+N +T+ F+  AD H LPATHV++ A ++IK YINST  P A I F+G
Sbjct: 428 EVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKG 487

Query: 486 TVIGDDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNV 545
           TVIG +  +PA+ASFSSRGP+L SPGILKPDI GPG++ILAAWPFPL N+T ++K TFN+
Sbjct: 488 TVIG-NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNST-DSKLTFNI 547

Query: 546 ISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFA 605
            SGTSMSCPHLSGIAAL+KSSHP WSPAAIKSAIMT+AD  N   + I+D+  QP DLFA
Sbjct: 548 ESGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFA 607

Query: 606 TGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGEL 665
           TG+GHVNPS+A DPGLVYDIQPDDYIPYLCGLGY + EV IIAH+ + CSA  SIPEGEL
Sbjct: 608 TGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCSA--SIPEGEL 667

Query: 666 NYPSFSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAY 725
           NYPSFSV LG  +TFTRTVTNVG  +  Y +++ A P GV V V+P K+ FS VNQK  Y
Sbjct: 668 NYPSFSVELGSSKTFTRTVTNVGEAHSSYDLIV-AAPQGVDVKVQPYKLNFSEVNQKETY 727

Query: 726 SVTFKRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 751
           SVTF R G +   +   AQ +LK +S+KH VRSPIS+KF+
Sbjct: 728 SVTFSRTG-LGNKTQEYAQGFLKWVSTKHTVRSPISVKFI 755

BLAST of Moc06g39670 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 9.0e-248
Identity = 449/748 (60.03%), Postives = 552/748 (73.80%), Query Frame = 0

Query: 6   SLFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLP---SSSE 65
           S+  L+ FH         S+ LQTYIVHV KP+         DLE+++ SFLP   S SE
Sbjct: 8   SIAFLLSFHL---TTAQRSTGLQTYIVHVDKPD-AQVLANSADLESYYNSFLPATVSGSE 67

Query: 66  DHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNRQF 125
              ++++S+ +V +GFAA+L+ E VK ME+K GF+SA+ E++L LHTTHTPNFLGL +  
Sbjct: 68  VPSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNM 127

Query: 126 GFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSA-CNNKLIGAR 185
           GFW++SN+GKG IIG+LD GITP HPSF D  MP PP KWKG+CEF  +A CN K+IGAR
Sbjct: 128 GFWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGAR 187

Query: 186 SFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLAH 245
           +F   +    G  PT      DE+GHGTHTASTAAG FV++A   GNA GTAVGMAPLAH
Sbjct: 188 NFISGS----GVPPT------DEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAH 247

Query: 246 LAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKGI 305
           +A+YKVC  + C D+DILAALDAA++DGVDVLSLSLG  S PF+ D+IAIGAFAA +KGI
Sbjct: 248 IAMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGI 307

Query: 306 FVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFPP 365
           FVS SA N GPLN+TLSNEAPWILTV AST DR+I+ATA LGNG+++DGES FQP+DFP 
Sbjct: 308 FVSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPH 367

Query: 366 TLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAMI 425
           TL LPLVY G  +E AA C  GSL   DVKGK VVC+RGG +AR+ K   VK+AGGAAMI
Sbjct: 368 TL-LPLVYPGTSDEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMI 427

Query: 426 LLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSPA 485
           L N + DG  T ADAHVLPATHV + A   IK YINST  P A I F+GT+IG    SP+
Sbjct: 428 LANLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKS-SPS 487

Query: 486 IASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPHL 545
           ++SFSSRGP+LASPGI+KPDI GPG++ILAAWP  ++N T  T  TFN+ISGTSMSCPHL
Sbjct: 488 VSSFSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKT-GTDLTFNIISGTSMSCPHL 547

Query: 546 SGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSKA 605
           SGI AL+KS+HPDWSPAAIKSAIMT+AD +N +G+PILD+   PAD+FATGAGHVNPSKA
Sbjct: 548 SGIVALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKA 607

Query: 606 TDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALGP 665
           +DPGL+YDIQ +DYI YLCGLGY++ ++ +I  + V C +  SI E ELNYPSFS+ LGP
Sbjct: 608 SDPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKCES--SISEAELNYPSFSIILGP 667

Query: 666 -PQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSI 725
             Q +TRTVTNVG     YTV I A   GV + V P  + F+ + Q+  Y+V+F + G  
Sbjct: 668 KTQNYTRTVTNVGDASSTYTVNI-AKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSGDF 727

Query: 726 TPPSSGSAQAYLKCMSSKHIVRSPISIK 749
           T       Q  +   S++++VRSPIS+K
Sbjct: 728 T---DRFVQGAISWSSNEYVVRSPISVK 732

BLAST of Moc06g39670 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 6.0e-244
Identity = 448/730 (61.37%), Postives = 537/730 (73.56%), Query Frame = 0

Query: 26  NLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSSSEDHPKLLYSFRNVMSGFAARLTEE 85
           NL TYIVHVKK E        E+L  WH SFLP +S +  ++++S+RNV SGFA RLT E
Sbjct: 37  NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPETS-NKDRMVFSYRNVASGFAVRLTPE 96

Query: 86  HVKAMEEKDGFISARRERILKLHTTHTPNFLGLNRQFGFWKDSNFGKGEIIGVLDGGITP 145
              A++EK+  +S R ER L LHTTHTP+FLGL +  G W DSN GKG IIGV+D GI P
Sbjct: 97  EANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGIYP 156

Query: 146 NHPSFDDAGMPPPPVKWKGRCEF-NFSACNNKLIGARSFNLATKALKGEQPTATDSPIDE 205
            H SF+D GMPPPP KWKG CEF   S CNNKLIGAR  NL   A++       + P ++
Sbjct: 157 FHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-------EPPYED 216

Query: 206 DGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLAHLAIYKVCFG---EDCPDSDILAA 265
             HGTHTA+ AAG FV+ A   GNA+GTA GMAP AHLAIYKVC     ++CP+S ILAA
Sbjct: 217 FFHGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAA 276

Query: 266 LDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKGIFVSCSASNSGPLNATLSNEA 325
           +D A+EDGVDVLSLSLG  S PFF D IAIGAFAATQKGIFVSCSA+NSGP  ++LSNEA
Sbjct: 277 MDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEA 336

Query: 326 PWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFPPTLFLPLVY-AGEKNETAALC 385
           PWILTV ASTIDR+I A+AKLGNG E++GE+LFQP DF   L LPLVY A EKN ++ALC
Sbjct: 337 PWILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQL-LPLVYAAAEKNNSSALC 396

Query: 386 GEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAMILLNQQTDGFTTEADAHVLP 445
             GSL++I+VKGK VVC+ GGGI  IAKG EV +AGG+AMIL N +  GFTT A+AHVLP
Sbjct: 397 APGSLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLP 456

Query: 446 ATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSPAIASFSSRGPSLASPGILKP 505
           A HV++ A+L IK YINST+ P A + F+GT+IG D  +P++A+FSSRGPS  SPGILKP
Sbjct: 457 AVHVSYAASLAIKAYINSTYTPTATVLFQGTIIG-DSLAPSVAAFSSRGPSQQSPGILKP 516

Query: 506 DITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPHLSGIAALIKSSHPDWSPAAI 565
           DI GPG++ILAAW   +DN      P F++ISGTSMSCPHLSGIAAL+KS+HPDWSPAAI
Sbjct: 517 DIIGPGVNILAAWAVSVDNKI----PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAI 576

Query: 566 KSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSKATDPGLVYDIQPDDYIPYLC 625
           KSAIMTTA+  N +G PILDQ  QPAD+FATGAGHVNP +A DPGLVYDIQP+DY+PYLC
Sbjct: 577 KSAIMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLC 636

Query: 626 GLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG-PPQTFTRTVTNVGGGYEVY 685
           GLGY D EV+II  + V C    SI + ELNYPSFS+ LG   Q +TRT+TNVG     Y
Sbjct: 637 GLGYSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTY 696

Query: 686 TVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSITPPSSGSAQAYLKCMSSKH 745
           TV I+  P  + ++V P +I F+ VNQKVAY V F         +   AQ  +  +S KH
Sbjct: 697 TVKIDV-PLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKH 749

Query: 746 IVRSPISIKF 750
           +VR+PIS+ F
Sbjct: 757 VVRTPISVIF 749

BLAST of Moc06g39670 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 3.7e-233
Identity = 427/732 (58.33%), Postives = 521/732 (71.17%), Query Frame = 0

Query: 26  NLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSSSEDHPKLLYSFRNVMSGFAARLTEE 85
           NL TYIVHVKK E ++++ Q EDL +W+ SFLP +     ++++S+R V SGFA +LT E
Sbjct: 34  NLMTYIVHVKKSENVASH-QSEDLHSWYHSFLPQTFPHKERMVFSYRKVASGFAVKLTPE 93

Query: 86  HVKAMEEKDGFISARRERILKLHTTHTPNFLGLNRQFGFWKDSNFGKGEIIGVLDGGITP 145
             K+++EK   +SAR ER L+LHTTHTP FLGL +  G W D N GKG IIG++D GI P
Sbjct: 94  EAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTGIFP 153

Query: 146 NHPSFDDAGMPPPPVKWKGRCEF-NFSACNNKLIGARSFNLATKALKGEQPTATDSPIDE 205
            HPSF+D GMPPPP KWKG CEF     CNNKLIGAR  NL   A++       + P + 
Sbjct: 154 LHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGAR--NLVKSAIQ-------EPPFEN 213

Query: 206 DGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLAHLAIYKVCFGE-DCPDSDILAALD 265
             HGTHTA+ AAG F+++A   GNAKG A GMAP AHLAIYKVC  +  C +S ILAA+D
Sbjct: 214 FFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMD 273

Query: 266 AAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKGIFVSCSASNSGPLNATLSNEAPW 325
            A+EDGVDVLSLSLG  S PFF D IAIGAFAATQ G+FVSCSA+NSGP  +TLSNEAPW
Sbjct: 274 IAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPW 333

Query: 326 ILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFPPTLFLPLVYA-----GEKNETAA 385
           ILTV ASTIDR+I+A+AKLGNGEE++GE+LFQP DF   L LPLVY      G + +  +
Sbjct: 334 ILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQL-LPLVYPGSFGYGNQTQNQS 393

Query: 386 LCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAMILLNQQTDGFTTEADAHV 445
           LC  GSLK+ID+ GK V+C+  G ++ I KG EV N+GG AMIL N +  GF+T A AHV
Sbjct: 394 LCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHV 453

Query: 446 LPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSPAIASFSSRGPSLASPGIL 505
           LPA  V++ A L IK YI ST+NP A + F+GT+IG D  +P++  FSSRGPS  SPGIL
Sbjct: 454 LPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIG-DSLAPSVVYFSSRGPSQESPGIL 513

Query: 506 KPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPHLSGIAALIKSSHPDWSPA 565
           KPDI GPG++ILAAW   +DN      P F+++SGTSMSCPHLSGIAALIKSSHPDWSPA
Sbjct: 514 KPDIIGPGVNILAAWAVSVDNKI----PAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPA 573

Query: 566 AIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSKATDPGLVYDIQPDDYIPY 625
           AIKSAIMTTA+  N  G PILDQ   PAD+FATGAGHVNP KA DPGLVYDI+P+DY+PY
Sbjct: 574 AIKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPY 633

Query: 626 LCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG-PPQTFTRTVTNVGGGYE 685
           LCGLGY D E+ +I    V CS   SIPE +LNYPSFS+ LG   Q +TRT+TNVG    
Sbjct: 634 LCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANS 693

Query: 686 VYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSITPPSSGSAQAYLKCMSS 745
            Y V +E  P  + ++V P +I F+ VN+KV++SV F         +    Q  L  +S 
Sbjct: 694 TYKVELEV-PLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSD 747

Query: 746 KHIVRSPISIKF 750
           +H VR PIS+ F
Sbjct: 754 RHAVRIPISVIF 747

BLAST of Moc06g39670 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 2.0e-231
Identity = 432/731 (59.10%), Postives = 520/731 (71.14%), Query Frame = 0

Query: 25  SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSSSE-DHPKLLYSFRNVMSGFAARLT 84
           SNL TYIVHV+KP+ +    Q +DL  ++ S LP S++  + ++++++RNV++GFA +LT
Sbjct: 40  SNLSTYIVHVRKPQVI----QSDDLHTFYYSLLPESTKTTNQRIVFTYRNVVNGFAVKLT 99

Query: 85  EEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNRQFGFWKDSNFGKGEIIGVLDGGI 144
            E  KA+++ +  +SAR E+IL LHTTHTP+FLGL +  G WK SN GKG IIG+LD GI
Sbjct: 100 PEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGI 159

Query: 145 TPNHPSFDDAGMPPPPVKWKGRCEFNFS-ACNNKLIGARSFNLATKALKGEQPTATDSPI 204
           +P HPSF D GMP PP KW G CEF     CNNK+IGAR+F + TK L          P 
Sbjct: 160 SPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNF-VKTKNL--------TLPF 219

Query: 205 DEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDSDILAAL 264
           D+ GHGTHTASTAAG  V  A   GNA GTAVGMAP AH+A+YKVC    C +S ILA +
Sbjct: 220 DDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGM 279

Query: 265 DAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKGIFVSCSASNSGPLNATLSNEAP 324
           D AV+DGVDVLSLSLG  S PFF D IA+GAF A QKGIFVSCSA+NSGP  ++LSNEAP
Sbjct: 280 DTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAP 339

Query: 325 WILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFPPTLFLPLVYAGE--KNETAALC 384
           WILTV AS+IDR I+ATAKLGNG+E+ G+S+FQP DF P+L LPLVYAG    N  +  C
Sbjct: 340 WILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSL-LPLVYAGANGNNNFSVFC 399

Query: 385 GEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAMILLNQQTDGFTTEADAHVLP 444
              SL   DV+GK V+CE GG + R+ KG  VK+AGGAAMIL+N   + F   AD HVLP
Sbjct: 400 APESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLP 459

Query: 445 ATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSPAIASFSSRGPSLASPGILKP 504
           A H+++ A L +K+YINST  P A I F GTVIG +  +P + SFSSRGPS ASPGILKP
Sbjct: 460 AVHISYEAGLALKEYINSTSTPTATILFEGTVIG-NLLAPQVTSFSSRGPSKASPGILKP 519

Query: 505 DITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPHLSGIAALIKSSHPDWSPAAI 564
           DI GPG++ILAAWP  LDN   +T P FN+ISGTSMSCPHLSGIAAL+K+SHPDWSPAAI
Sbjct: 520 DIIGPGLNILAAWPVSLDN---STTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAI 579

Query: 565 KSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSKATDPGLVYDIQPDDYIPYLC 624
           KSAIMTTA   N  G PILDQ   PAD+FATGAGHVNP KA DPGLVYDI+P+DYIPYLC
Sbjct: 580 KSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLC 639

Query: 625 GLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG-PPQTFTRTVTNVGGGYEVY 684
           GL Y D EV +I  Q V CS    I E ELNYPSFS+ LG   Q +TRTV NVG     Y
Sbjct: 640 GLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTY 699

Query: 685 TVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSITPPSSGSAQAYLKCMSSKH 744
           T  I   P GV +++ P ++ F+ V QK+ YSV+F    S    +   AQ  LK +S K+
Sbjct: 700 TAEI-GVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPF-SEDRDNHTFAQGSLKWVSGKY 750

Query: 745 IVRSPISIKFV 751
            VRSPIS  F+
Sbjct: 760 SVRSPISFIFL 750

BLAST of Moc06g39670 vs. ExPASy TrEMBL
Match: A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 748/750 (99.73%), Postives = 748/750 (99.73%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSS 60
           MVVLPSLFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSS
Sbjct: 1   MVVLPSLFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSS 60

Query: 61  SEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNR 120
           SEDHPKLLYSF NVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNR
Sbjct: 61  SEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNR 120

Query: 121 QFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKG IIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLA 240
           RSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLA
Sbjct: 181 RSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKG 300
           HLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKG
Sbjct: 241 HLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKG 300

Query: 301 IFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFP 360
           IFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFP
Sbjct: 301 IFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFP 360

Query: 361 PTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAM 420
           PTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAM
Sbjct: 361 PTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAM 420

Query: 421 ILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSP 480
           ILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSP
Sbjct: 421 ILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSP 480

Query: 481 AIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPH 540
           AIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPH
Sbjct: 481 AIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMSCPH 540

Query: 541 LSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSK 600
           LSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSK
Sbjct: 541 LSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPSK 600

Query: 601 ATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG 660
           ATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG
Sbjct: 601 ATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG 660

Query: 661 PPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSI 720
           PPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSI
Sbjct: 661 PPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSI 720

Query: 721 TPPSSGSAQAYLKCMSSKHIVRSPISIKFV 751
           TPPSSGSAQAYLKCMSSKHIVRSPISIKFV
Sbjct: 721 TPPSSGSAQAYLKCMSSKHIVRSPISIKFV 750

BLAST of Moc06g39670 vs. ExPASy TrEMBL
Match: A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 623/752 (82.85%), Postives = 668/752 (88.83%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV  PSLFLL+L +FH  GA  T     NLQTYIVHVKKPE        +DLE WHRSFL
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPE------TTDDLEIWHRSFL 60

Query: 61  PSSSEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 120
           PSSS     LLYS+RNVMSGFAARL+EE VKAMEE DGF+SARRERIL+LHTTHTP+FLG
Sbjct: 61  PSSS----SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLG 120

Query: 121 LNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKL 180
           LNRQFGFWKDSNFGKG IIGVLDGGI P+HPSFDD GMPPPP KWKGRCEFNFSACNNKL
Sbjct: 121 LNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKL 180

Query: 181 IGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMA 240
           IGARSFNLATKALKGE     DSPIDEDGHGTHTASTAAGAFV  AEALGNAKGTAVGMA
Sbjct: 181 IGARSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMA 240

Query: 241 PLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAAT 300
           PLAHLAIYKVCFGEDCPD+DILAALDAA+EDGVDVLSLSLGS S PFF+D +AIGAFAA 
Sbjct: 241 PLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAI 300

Query: 301 QKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPS 360
           QKGIFVSCSA+NSGP  ATLSNEAPWILTVAASTIDRRI A AKLGNGEEFDGESLFQPS
Sbjct: 301 QKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPS 360

Query: 361 DFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGG 420
           DFPPTL LPLVYAGEKN+TAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EVKNAGG
Sbjct: 361 DFPPTL-LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGG 420

Query: 421 AAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDD 480
           AAMILLNQQ DGF+TEADAHVLPA+HV+H AALKIK YINST  P A I F+GTVIGDD+
Sbjct: 421 AAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDN 480

Query: 481 FSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMS 540
           FSPAIASFSSRGPS+ASPGILKPDITGPG+SILAAWPFPLD N +NTK TFN+ISGTSMS
Sbjct: 481 FSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKN-RNTKSTFNIISGTSMS 540

Query: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVN 600
           CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITN +G+PI+D+N QPADLFATGAGHVN
Sbjct: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVN 600

Query: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSV 660
           PSKA DPGLVYDIQPDDYIPYLCGLGYK NEV+ IA +P++C AKPSIPEG+LNYPSF+V
Sbjct: 601 PSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTV 660

Query: 661 ALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRI 720
            LGPPQTFTRTVTNVG G EVYT ++EAPPS +SVT+RP KIFFS +NQKV +SVTFKRI
Sbjct: 661 VLGPPQTFTRTVTNVGCGREVYTAVVEAPPS-ISVTIRPSKIFFSKINQKVTFSVTFKRI 720

Query: 721 GSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 750
           GSI+ PS+   + YLK +S KH+VRSPIS KF
Sbjct: 721 GSIS-PSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Moc06g39670 vs. ExPASy TrEMBL
Match: A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 608/756 (80.42%), Postives = 659/756 (87.17%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV+LPSLFLL+L +FHG  A+ T    SNL TYIVHVKKPE       V+DLE WHRSFL
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPE------VVDDLEIWHRSFL 60

Query: 61  PSS---SEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPN 120
           P+S    E+ P LLYS+RNVMSGF+ARLTEEHVKAMEEKDGF+SARRE I+ LHTTHTP+
Sbjct: 61  PTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPD 120

Query: 121 FLGLNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACN 180
           FLGLNRQFGFWKDSNFGKG IIGVLDGGITPNHPSFDDAGMP PP KWKGRCEFNFSACN
Sbjct: 121 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACN 180

Query: 181 NKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAV 240
           NKLIGARS NLA++ALKG+  T  DSPIDEDGHGTHTASTAAG FVD AEALGNA GTAV
Sbjct: 181 NKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAV 240

Query: 241 GMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAF 300
           GMAPLAHLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG  S PFF D  AIG+F
Sbjct: 241 GMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSF 300

Query: 301 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 360
           AA QKGIFVSCSA+NSGP NATLSNEAPWILTVAASTIDR+I ATAKLGNGEEFDGESLF
Sbjct: 301 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 360

Query: 361 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 420
           QP+DFP TL LPLV+ GEKNET ALC EGSLK+IDVKGK VVCERGGGIARIAKG+EVKN
Sbjct: 361 QPNDFPQTL-LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 420

Query: 421 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 480
            GGAAMILLN ++DGFTTE DAHVLPA+HV+H AALKIK YINST  P A I F+GT IG
Sbjct: 421 GGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIG 480

Query: 481 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGT 540
            DDFSPAIASFSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT NTK TFN+ISGT
Sbjct: 481 -DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT-NTKSTFNIISGT 540

Query: 541 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAG 600
           SMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTA+ITN +G PILD+  QPADLFA GAG
Sbjct: 541 SMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAG 600

Query: 601 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPS 660
           HVNPSKA DPGLVYDIQPDDYIPYLCGLGY +N+VS+IAH+P+DC    SIPEGELNYPS
Sbjct: 601 HVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPS 660

Query: 661 FSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTF 720
           F V LGP QTF+RTVT+VG G  VY V+IEA P GVSVTVRPRK+ FSA+NQK  YSVTF
Sbjct: 661 FMVKLGPVQTFSRTVTSVGSGRVVYNVVIEA-PEGVSVTVRPRKLSFSALNQKATYSVTF 720

Query: 721 KRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 751
           KRIGSI+ PS   A+ YLK +S+KH+VRSPIS+KFV
Sbjct: 721 KRIGSIS-PSIEFAEGYLKWVSAKHVVRSPISVKFV 745

BLAST of Moc06g39670 vs. ExPASy TrEMBL
Match: A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)

HSP 1 Score: 1183.7 bits (3061), Expect = 0.0e+00
Identity = 606/756 (80.16%), Postives = 657/756 (86.90%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV+LPSLFLL+L +FHG  A+ T    SNL TYIVHVKKPE       V+DLE WHRSFL
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPE------VVDDLEIWHRSFL 60

Query: 61  PSS---SEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPN 120
           P+S    E+ P LLYS+RNVMSGF+ARLTEEHVKAMEEKDGF+SARRE I+ LHTTHTP+
Sbjct: 61  PTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPD 120

Query: 121 FLGLNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACN 180
           FLGLNRQFGFWKDSNFGKG IIGVLDGGITPNHPSFDDAGM  PP KWKGRCEFNFSACN
Sbjct: 121 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACN 180

Query: 181 NKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAV 240
           NKLIGARS NLA++ALKG+  T  DSPIDEDGHGTHTASTAAG FVD AEALGNA GTAV
Sbjct: 181 NKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAV 240

Query: 241 GMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAF 300
           GMAPLAHLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG  S PFF D  AIG+F
Sbjct: 241 GMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSF 300

Query: 301 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 360
           AA QKGIFVSCSA+NSGP NATLSNEAPWILTVAASTIDR+I ATAKLGNGEEFDGESLF
Sbjct: 301 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 360

Query: 361 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 420
           QP+DFP TL LPLV+ GEKNET ALC EGSLK+IDVKGK VVCERGGGIARIAKG+EVKN
Sbjct: 361 QPNDFPQTL-LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 420

Query: 421 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 480
            GGAAMILLN ++DGFTTE DAHVLPA+HV+H AALKIK YINST  P A I F+GT IG
Sbjct: 421 GGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIG 480

Query: 481 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGT 540
            DDFSPAIASFSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT NTK TFN+ISGT
Sbjct: 481 -DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT-NTKSTFNIISGT 540

Query: 541 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAG 600
           SMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTA+ITN +G PILD+  QPADLFA GAG
Sbjct: 541 SMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAG 600

Query: 601 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPS 660
           HVNPSKA DPGLVYDIQPDDYIPYLCGLGY +N+VS+IAH+P+DC    SIPEGELNYPS
Sbjct: 601 HVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPS 660

Query: 661 FSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTF 720
           F V LGP QTF+RTVT+VG G  VY V+IEA P GVSVTVRPRK+ FSA+NQK  YSVTF
Sbjct: 661 FMVKLGPVQTFSRTVTSVGSGRVVYNVVIEA-PEGVSVTVRPRKLSFSALNQKATYSVTF 720

Query: 721 KRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 751
           KR GSI+ PS   A+ YLK +S+KH+VRSPIS+KFV
Sbjct: 721 KRSGSIS-PSIEFAEGYLKWVSAKHVVRSPISVKFV 745

BLAST of Moc06g39670 vs. ExPASy TrEMBL
Match: A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 603/756 (79.76%), Postives = 659/756 (87.17%), Query Frame = 0

Query: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
           MV+LPSLFLL+L +FH   A+ T    SNL TYIVHVKKPE       V+DLE+WHRSFL
Sbjct: 1   MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPE------VVDDLESWHRSFL 60

Query: 61  PSS---SEDHPKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPN 120
           P+S   SE+ P LLYS+RNVMSGF+ARLTEEHVKAMEEKDGF+SARRE I+ LHTTH+PN
Sbjct: 61  PTSLENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPN 120

Query: 121 FLGLNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACN 180
           FLGLNRQFGFWKDSNFGKG IIGVLDGGITP+HPSF DAGMP PP KWKGRCEFNFSACN
Sbjct: 121 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACN 180

Query: 181 NKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAV 240
           NKLIGARS NLA++ALKG+  T  DSPIDEDGHGTHTASTAAG FVD AEALGNA GTAV
Sbjct: 181 NKLIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAV 240

Query: 241 GMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAF 300
           GMAPLAHLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG    PFF D  AIGAF
Sbjct: 241 GMAPLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAF 300

Query: 301 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 360
           AA QKGIFVSCSA+NSGP NATLSNEAPWILTVAASTIDR+I ATAKLGNGEEFDGESLF
Sbjct: 301 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 360

Query: 361 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 420
           QP+DFP T FLPLV+ GEKNET ALC EGSLK+IDVKGK VVC+RGGGIARIAKG+EVKN
Sbjct: 361 QPNDFPQT-FLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKN 420

Query: 421 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 480
           AGGAAMILLN ++DGFTTEADAHVLPA+HV+H AALKIK YINST  P A I F+GT IG
Sbjct: 421 AGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIG 480

Query: 481 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGT 540
            DDFSPAIA+FSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT NTK TFN++SGT
Sbjct: 481 -DDFSPAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT-NTKSTFNIVSGT 540

Query: 541 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAG 600
           SMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTA+ITN +G PI+DQ  QPADLFA GAG
Sbjct: 541 SMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAG 600

Query: 601 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPS 660
           HVNPSKA DPGLVYDIQPDDYIPYLCGLGY +N+VS+IAH+P+DC    SIPEGELNYPS
Sbjct: 601 HVNPSKAVDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPS 660

Query: 661 FSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTF 720
           F V LG  QTF+RTVT VG G EVY V+IEA P GVSVTVRPRK+ FSA+NQK  YSVTF
Sbjct: 661 FMVKLGQVQTFSRTVTYVGSGREVYNVVIEA-PEGVSVTVRPRKVIFSALNQKATYSVTF 720

Query: 721 KRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 751
           KRIGSI+ PS+  A+ YLK +S+KH+VRSPIS+KFV
Sbjct: 721 KRIGSIS-PSTEFAEGYLKWVSAKHLVRSPISVKFV 745

BLAST of Moc06g39670 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 585.5 bits (1508), Expect = 6.1e-167
Identity = 338/745 (45.37%), Postives = 465/745 (62.42%), Query Frame = 0

Query: 23  TSSNLQTYIVHVKKPEKLSTYGQVEDL-ENWHRSFLPSSSEDHPKLLYSFRNVMSGFAAR 82
           +SS+  TYIVH+ K +  S++    DL  NW+ S L S S D  +LLY++ N + GF+ R
Sbjct: 25  SSSDQGTYIVHMAKSQMPSSF----DLHSNWYDSSLRSIS-DSAELLYTYENAIHGFSTR 84

Query: 83  LTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNRQFG-FWKDSNFGKGEIIGVLD 142
           LT+E   ++  + G IS   E   +LHTT TP FLGL+      + ++      ++GVLD
Sbjct: 85  LTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLD 144

Query: 143 GGITPNHPSFDDAGMPPPPVKWKGRCE----FNFSACNNKLIGARSFNLATKALKG--EQ 202
            G+ P   S+ D G  P P  WKG CE    F  S CN KLIGAR F    ++  G  ++
Sbjct: 145 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDE 204

Query: 203 PTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCP 262
              + SP D+DGHGTHT+STAAG+ V+ A  LG A GTA GMAP A +A+YKVC+   C 
Sbjct: 205 SKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCF 264

Query: 263 DSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKGIFVSCSASNSGPLN 322
            SDILAA+D A+ D V+VLS+SLG   + ++RD +AIGAFAA ++GI VSCSA N+GP +
Sbjct: 265 SSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSS 324

Query: 323 ATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFPPTLFLPLVYAGEKN 382
           ++LSN APWI TV A T+DR   A A LGNG+ F G SLF+    P  L LP +YAG  +
Sbjct: 325 SSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKL-LPFIYAGNAS 384

Query: 383 E--TAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAMILLNQQTDGFTT 442
                 LC  G+L    VKGK V+C+RG   AR+ KG  VK AGG  MIL N   +G   
Sbjct: 385 NATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAANGEEL 444

Query: 443 EADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSPAIASFSSRGPSL 502
            ADAH+LPAT V   A   I+ Y+ +  NP A+I+  GTV+G    SP +A+FSSRGP+ 
Sbjct: 445 VADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP-SPVVAAFSSRGPNS 504

Query: 503 ASPGILKPDITGPGISILAAW-----PFPLDNNTKNTKPTFNVISGTSMSCPHLSGIAAL 562
            +P ILKPD+  PG++ILAAW     P  L ++++  +  FN+ISGTSMSCPH+SG+AAL
Sbjct: 505 ITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVE--FNIISGTSMSCPHVSGLAAL 564

Query: 563 IKSSHPDWSPAAIKSAIMTTADITNHKGRPILD-QNFQPADLFATGAGHVNPSKATDPGL 622
           +KS HP+WSPAAI+SA+MTTA  T   G+P+LD    +P+  F  GAGHV+P+ AT+PGL
Sbjct: 565 LKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGL 624

Query: 623 VYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSVALG--PPQT 682
           +YD+  +DY+ +LC L Y   ++  ++ +   C    S    +LNYPSF+V +       
Sbjct: 625 IYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYK 684

Query: 683 FTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRIGSITPPS 742
           +TRTVT+VGG    Y+V + +  +GV ++V P  + F   N+K +Y+VTF    S   PS
Sbjct: 685 YTRTVTSVGGA-GTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSS--KPS 744

Query: 743 SGSAQAYLKCMSSKHIVRSPISIKF 750
             ++   ++    KH+V SP++I +
Sbjct: 745 GSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Moc06g39670 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 584.3 bits (1505), Expect = 1.4e-166
Identity = 347/772 (44.95%), Postives = 465/772 (60.23%), Query Frame = 0

Query: 7   LFLLVLFHFHGGGARPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPS--SSEDH 66
           +F L+L  F    +  +S  L++YIVHV++  K S +       NWH S L S  SS   
Sbjct: 12  VFPLLLCFFSPSSS--SSDGLESYIVHVQRSHKPSLF---SSHNNWHVSLLRSLPSSPQP 71

Query: 67  PKLLYSFRNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNRQFGF 126
             LLYS+   + GF+ARL+     A+      IS   ++  ++HTTHTP FLG ++  G 
Sbjct: 72  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 131

Query: 127 WKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCE----FNFSACNNKLIGA 186
           W +SN+G+  I+GVLD GI P HPSF D+G+ P P  WKG CE    F  S+CN KLIGA
Sbjct: 132 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 191

Query: 187 RSFN----LATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGM 246
           R+F           K      + SP D +GHGTHTASTAAG+ V  A     A+GTA GM
Sbjct: 192 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 251

Query: 247 APLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLG-SDSAP-FFRDDIAIGAF 306
           A  A +A YK+C+   C DSDILAA+D AV DGV V+SLS+G S SAP +  D IAIGAF
Sbjct: 252 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 311

Query: 307 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 366
            AT+ GI VSCSA NSGP   T +N APWILTV AST+DR   A A  G+G+ F G SL+
Sbjct: 312 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 371

Query: 367 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 426
                P +  L LVY+G+    + LC  G L    V+GK V+C+RGG  AR+ KG  VK 
Sbjct: 372 AGESLPDSQ-LSLVYSGDCG--SRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKL 431

Query: 427 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 486
           AGGA MIL N    G    AD+H++PAT V   A  +I+DYI ++ +P A I+F GT+IG
Sbjct: 432 AGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG 491

Query: 487 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWP---FPLDNNTKNTKPTFNVI 546
               SP +A+FSSRGP+  +P ILKPD+  PG++ILA W     P D +    +  FN+I
Sbjct: 492 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 551

Query: 547 SGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILD-QNFQPADLFA 606
           SGTSMSCPH+SG+AAL++ +HPDWSPAAIKSA++TTA    + G PI D    + ++ F 
Sbjct: 552 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFI 611

Query: 607 TGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPV---DCSAKPSIPE 666
            GAGHV+P+KA +PGLVYDI+  +Y+ +LC +GY+   + +    P     C        
Sbjct: 612 HGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTA 671

Query: 667 GELNYPSFSV---ALGPPQTFTRTVTNVGGGYE-VYTVMIEAPPSGVSVTVRPRKIFFSA 726
           G+LNYPSFSV   + G    + R V NVG   + VY V +++ P+ V + V P K+ FS 
Sbjct: 672 GDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKS-PANVEIDVSPSKLAFSK 731

Query: 727 VNQKVAYSVTFKR------IGSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 750
               + Y VTFK       +GS+     GS    ++    +H+V+SP+++++
Sbjct: 732 EKSVLEYEVTFKSVVLGGGVGSVPGHEFGS----IEWTDGEHVVKSPVAVQW 769

BLAST of Moc06g39670 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 574.3 bits (1479), Expect = 1.4e-163
Identity = 337/760 (44.34%), Postives = 458/760 (60.26%), Query Frame = 0

Query: 28  QTYIVHV-KKPEKLSTYGQVEDLENWHRSFLPSS------SEDHP--KLLYSFRNVMSGF 87
           QTYIV +    E   T+    D   WH SFL  +       E+ P  +LLYS+ + + GF
Sbjct: 26  QTYIVQLHPNSETAKTFASKFD---WHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGF 85

Query: 88  AARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLN--RQFGFWKDSNFGKGEII 147
           AA+LTE   + +      ++ R + +L++ TT++  FLGL+     G W  S FG+G II
Sbjct: 86  AAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTII 145

Query: 148 GVLDGGITPNHPSFDDAGMPPPPVKWKGRCE----FNFSACNNKLIGARSFNLATKALKG 207
           GVLD G+ P  PSFDD GMP  P KWKG C+    F+ S+CN KLIGAR F    +    
Sbjct: 146 GVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANS 205

Query: 208 EQ-----PTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLAHLAIYKV 267
            +     P    S  D  GHGTHTAST  G+ V  A  LGN  G A GMAP AH+A+YKV
Sbjct: 206 PEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKV 265

Query: 268 CFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKGIFVSCSA 327
           C+   C  SDILAA+D A++D VDVLSLSLG    P + D IAIG F A ++GI V C+A
Sbjct: 266 CWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAA 325

Query: 328 SNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFPPT-LFLP 387
            N+GP+ ++++N APW+ T+ A T+DRR  A  +L NG+   GESL+           + 
Sbjct: 326 GNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVE 385

Query: 388 LVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAMILLNQQ 447
           ++Y    ++ +  C  GSL   +++GK V+C+RG    R  KG  VK AGG AMIL N +
Sbjct: 386 VIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTE 445

Query: 448 TDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDDFSPAIASFS 507
            +      D H+LPAT + +  ++ +K Y+N+T  P A I F GTVIG    +P +A FS
Sbjct: 446 INQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSR-APEVAQFS 505

Query: 508 SRGPSLASPGILKPDITGPGISILAAWP-------FPLDNNTKNTKPTFNVISGTSMSCP 567
           +RGPSLA+P ILKPD+  PG++I+AAWP        P D+   N    F V+SGTSMSCP
Sbjct: 506 ARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVN----FTVMSGTSMSCP 565

Query: 568 HLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVNPS 627
           H+SGI ALI+S++P+WSPAAIKSA+MTTAD+ + +G+ I D N +PA +FA GAGHVNP 
Sbjct: 566 HVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQ 625

Query: 628 KATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSA-KPSIPEGELNYPSFSVA 687
           KA +PGLVY+IQP DYI YLC LG+  +++  I H+ V C+      P   LNYPS +V 
Sbjct: 626 KAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVI 685

Query: 688 LGPPQT---FTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTF- 747
               +T    TR VTNVG    +Y+V ++A P G+ V V P+++ F  V+Q ++Y V F 
Sbjct: 686 FKRGKTTEMITRRVTNVGSPNSIYSVNVKA-PEGIKVIVNPKRLVFKHVDQTLSYRVWFV 745

BLAST of Moc06g39670 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 572.4 bits (1474), Expect = 5.4e-163
Identity = 335/755 (44.37%), Postives = 447/755 (59.21%), Query Frame = 0

Query: 23  TSSNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFLPSSSEDHPKLLYSFRNVMSGFAARL 82
           +SSN  TYIVHV    K S +       +W+ S L S +   P +++++  V  GF+ARL
Sbjct: 21  SSSNSLTYIVHVDHEAKPSIF---PTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARL 80

Query: 83  TEEHVKAMEEKDGFISARRERILKLHTTHTPNFLGLNR--QFGFWKDSNFGKGEIIGVLD 142
           T +    + +    IS   E++  LHTT +P FLGL    + G  ++S+FG   +IGV+D
Sbjct: 81  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 140

Query: 143 GGITPNHPSFDDAGMPPPPVKWKGRC----EFNFSACNNKLIGARSFNLATKALKGEQPT 202
            G+ P  PSFDD G+ P P+KWKG+C    +F  SACN KL+GAR F    +A  G+   
Sbjct: 141 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 200

Query: 203 ATD--SPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCP 262
            T+  SP D DGHGTHTAS +AG +V  A  LG A G A GMAP A LA YKVC+   C 
Sbjct: 201 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 260

Query: 263 DSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAATQKGIFVSCSASNSGPLN 322
           DSDILAA D AV DGVDV+SLS+G    P++ D IAIGAF A  +GIFVS SA N GP  
Sbjct: 261 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 320

Query: 323 ATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPSDFPPTLFLPLVYAGE-- 382
            T++N APW+ TV A TIDR   A  KLGNG+   G S++      P    PLVY G   
Sbjct: 321 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLL 380

Query: 383 --KNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGGAAMILLNQQTDGF 442
                +++LC EGSL    VKGK V+C+RG   +R  KG  V+  GG  MI+ N   DG 
Sbjct: 381 GGDGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRKNGGLGMIIANGVFDGE 440

Query: 443 TTEADAHVLPATHVNHVAALKIKDYIN------STHNPIAAIAFRGTVIGDDDFSPAIAS 502
              AD HVLPAT V      +I+ YI+      S+ +P A I F+GT +G    +P +AS
Sbjct: 441 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRP-APVVAS 500

Query: 503 FSSRGPSLASPGILKPDITGPGISILAAWP---FPLDNNTKNTKPTFNVISGTSMSCPHL 562
           FS+RGP+  +P ILKPD+  PG++ILAAWP    P    + N +  FN++SGTSM+CPH+
Sbjct: 501 FSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHV 560

Query: 563 SGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNF-QPADLFATGAGHVNPSK 622
           SG+AAL+K++HPDWSPAAI+SA++TTA   ++ G P++D++    + +   G+GHV+P+K
Sbjct: 561 SGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTK 620

Query: 623 ATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDC-SAKPSIPEGELNYPSFSVAL 682
           A DPGLVYDI   DYI +LC   Y    +  I  +  DC  A+ +   G LNYPSFSV  
Sbjct: 621 AMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVF 680

Query: 683 GP------PQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVT 742
                      F RTVTNVG    VY + I  PP G +VTV P K+ F  V QK+++ V 
Sbjct: 681 QQYGESKMSTHFIRTVTNVGDSDSVYEIKIR-PPRGTTVTVEPEKLSFRRVGQKLSFVVR 740

Query: 743 FKRIG-SITPPSSGSAQAYLKCMSSKHIVRSPISI 748
            K     ++P ++     ++     K  V SP+ +
Sbjct: 741 VKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVV 769

BLAST of Moc06g39670 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 571.2 bits (1471), Expect = 1.2e-162
Identity = 341/771 (44.23%), Postives = 463/771 (60.05%), Query Frame = 0

Query: 2   VVLPSLFLLVLFHFHGGGARPTSSNLQTYIV---HVKKPEKLSTYGQVEDLENWHRSFLP 61
           + + + FL +L H         ++  +TYI+   H  KPE   T+       +W+ S L 
Sbjct: 11  ITIITTFLFLLLH---------TTAKKTYIIRVNHSDKPESFLTH------HDWYTSQLN 70

Query: 62  SSSEDHPKLLYSFRNVMSGFAARL-TEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 121
           S S     LLY++     GF+A L + E    +   +  +    + +  LHTT TP FLG
Sbjct: 71  SES----SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLG 130

Query: 122 LNRQFGFWKDSNFGKGEIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCE----FNFSAC 181
           LN +FG     +   G IIGVLD G+ P   SFDD  MP  P KWKG CE    F+   C
Sbjct: 131 LNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 190

Query: 182 NNKLIGARSFNLATKALKG---EQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAK 241
           N KLIGARSF+   +   G        + SP D DGHGTHT++TAAG+ V  A  LG A 
Sbjct: 191 NKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAA 250

Query: 242 GTAVGMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIA 301
           GTA GMA  A +A YKVC+   C  SDILAA+D A+ DGVDVLSLSLG  SAP++RD IA
Sbjct: 251 GTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIA 310

Query: 302 IGAFAATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDG 361
           IGAF+A ++G+FVSCSA NSGP  A+++N APW++TV A T+DR   A A LGNG+   G
Sbjct: 311 IGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTG 370

Query: 362 ESLFQPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGM 421
            SL+       T  L LVY    + ++ LC  GSL    V+GK VVC+RG   AR+ KG 
Sbjct: 371 VSLYSGVGM-GTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGA 430

Query: 422 EVKNAGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRG 481
            V++AGG  MI+ N    G    AD+H+LPA  V       +++Y+ S   P A + F+G
Sbjct: 431 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKG 490

Query: 482 TVIGDDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAW-----PFPLDNNTKNTK 541
           TV+ D   SP +A+FSSRGP+  +P ILKPD+ GPG++ILA W     P  LD +++ T+
Sbjct: 491 TVL-DVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQ 550

Query: 542 PTFNVISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILD-QNFQ 601
             FN++SGTSMSCPH+SG+A L+K++HP+WSP+AIKSA+MTTA + ++   P+ D  +  
Sbjct: 551 --FNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNS 610

Query: 602 PADLFATGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYK-DNEVSIIAHQPVDCSAKP 661
            ++ +A G+GHV+P KA  PGLVYDI  ++YI +LC L Y  D+ V+I+    V+CS K 
Sbjct: 611 LSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKF 670

Query: 662 SIPEGELNYPSFSVALGPPQT--FTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFF 721
           S P G+LNYPSFSV  G  +   +TR VTNVG    VY V +   PS V ++V+P K+ F
Sbjct: 671 SDP-GQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPS-VGISVKPSKLSF 730

Query: 722 SAVNQKVAYSVTF---KRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 750
            +V +K  Y+VTF   K +        GS    +   + +H VRSP++  +
Sbjct: 731 KSVGEKKRYTVTFVSKKGVSMTNKAEFGS----ITWSNPQHEVRSPVAFSW 751