Moc06g38120 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g38120
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RING-type domain-containing protein
Location	chr6: 29462278 .. 29463780 (-)
RNA-Seq Expression	Moc06g38120
Synteny	Moc06g38120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGGTTGCTGCTGTTGTTCTTCAAAAGGAACAGAATCAAATATTGCACCATCCTACTATTATGTCAGTATTGAATTTACCATTGGATGTTTATTTTCATATTTTTATCAATCAAATTCATTATTGTAAGCAAACACTGCAGCCATTATCTAGTATTCATTCATTTCTTAATTATCATTGCCAACAGGCCTAGTTGCATATAATTGTAGGTATTCTGTTATAGCCTGTGAGATAGTGAAGGTCATTTACATCATTTGAAACCCAAAACAGAGAGTGATTTTTATAATGAACTATATTTTCTATAAGTATTTTTATGCATTATGTTGCATAATTCATCATCCATGTATTTTCCCGAGAAGTCTTCTGCCTTCGCACGAATTAACTTAGATTGGTGGTGATCATAGGAGACGCTTGTGTGCCATACTTAATAAATCATGTAAGTTGCAGATTTAACATTTTAAGTGAATATAATAAACATTACATACACTCAAAAACTATCAGTCTATCACTTCTGTTATTTGATCTTGGAACTAGTTGAACTATATGCTGAACTGTTAGCACGATAGTGAAAAGAGTTTTCTTGCCATTGTAAACTGCCTTGCTCAATTTACTTGAATTCAAGTTTTGATGCAGTACCCAAGAGCATCAGATGAGCATGTTCCCCTGTCATCTCCAATTGGTGCTCCCTCAGCATTTTCTAGCGGGCTTTTGGTCGATACAAATCTAGATACATCAATACCTGACACTTATAGATCACCTCCCTTGCCTATGCCATATGATGTTGTTTTAACACATCCACATACTCCACCTGTGGCTCAAGAAATTGGCAGCAATAAGAATGATGCTGCAGCCCAGACAACAAATTCTGATGCAGTTCAAGAAATCGCATGCACTAATGCTCGAGAAACTTCAGCCAAGTGTGAAGGCATCGATGAATCAGACTGCAAGAAGCATACAGACTTTGAAACAGACACATTAAAGGAATCTGAAATCGAACTTTCGAAGGGTGTAGAATCTGTGGCCTTAGCAATTGAAGAGGAGGATGTTTGTCCCATCTGTTTGGAAGGTGAAGTTTTGCCAAAACTTCGTTCAGCTGCCTAGTACCAACCCCACCAAACTATGGAAACATCTTTAACCTCTTATTTTCCAATGTTTGACCTTTTAGTTGCTTGAACTAACAAGTCACCACATTATCGCTTTCTCGATAGGCTGCAGTAGCTTTCCCTTTCGATTCATCCTTTTCTATTATTTTATGCAGAATATGATGGGGAGAATCCAAAACTTACCACAAAATGCGAGCATCATTTTCATCTTGCATGCATTCTGGAATGGATGGAAAGAAGTGATATCTGTCCCGTGTGCGATCAGGTGTGTGCCTCACTTTACATTTAACGTTTATTTCTACTCATTAGCTCGTAAAACCATAAAGAAAACTTTCTTATCTCTTCATCATCCCCCCCTGAAATGCAGGAAATGCTCTTTAGCCCTCCAATTGATTAG

mRNA sequence

ATGGGAGGTTGCTGCTGTTGTTCTTCAAAAGGAACAGAATCAAATATTGCACCATCCTACTATTATTACCCAAGAGCATCAGATGAGCATGTTCCCCTGTCATCTCCAATTGGTGCTCCCTCAGCATTTTCTAGCGGGCTTTTGGTCGATACAAATCTAGATACATCAATACCTGACACTTATAGATCACCTCCCTTGCCTATGCCATATGATGTTGTTTTAACACATCCACATACTCCACCTGTGGCTCAAGAAATTGGCAGCAATAAGAATGATGCTGCAGCCCAGACAACAAATTCTGATGCAGTTCAAGAAATCGCATGCACTAATGCTCGAGAAACTTCAGCCAAGTGTGAAGGCATCGATGAATCAGACTGCAAGAAGCATACAGACTTTGAAACAGACACATTAAAGGAATCTGAAATCGAACTTTCGAAGGGTGTAGAATCTGTGGCCTTAGCAATTGAAGAGGAGGATGTTTGTCCCATCTGTTTGGAAGAATATGATGGGGAGAATCCAAAACTTACCACAAAATGCGAGCATCATTTTCATCTTGCATGCATTCTGGAATGGATGGAAAGAAGTGATATCTGTCCCGTGTGCGATCAGGAAATGCTCTTTAGCCCTCCAATTGATTAG

Coding sequence (CDS)

ATGGGAGGTTGCTGCTGTTGTTCTTCAAAAGGAACAGAATCAAATATTGCACCATCCTACTATTATTACCCAAGAGCATCAGATGAGCATGTTCCCCTGTCATCTCCAATTGGTGCTCCCTCAGCATTTTCTAGCGGGCTTTTGGTCGATACAAATCTAGATACATCAATACCTGACACTTATAGATCACCTCCCTTGCCTATGCCATATGATGTTGTTTTAACACATCCACATACTCCACCTGTGGCTCAAGAAATTGGCAGCAATAAGAATGATGCTGCAGCCCAGACAACAAATTCTGATGCAGTTCAAGAAATCGCATGCACTAATGCTCGAGAAACTTCAGCCAAGTGTGAAGGCATCGATGAATCAGACTGCAAGAAGCATACAGACTTTGAAACAGACACATTAAAGGAATCTGAAATCGAACTTTCGAAGGGTGTAGAATCTGTGGCCTTAGCAATTGAAGAGGAGGATGTTTGTCCCATCTGTTTGGAAGAATATGATGGGGAGAATCCAAAACTTACCACAAAATGCGAGCATCATTTTCATCTTGCATGCATTCTGGAATGGATGGAAAGAAGTGATATCTGTCCCGTGTGCGATCAGGAAATGCTCTTTAGCCCTCCAATTGATTAG

Protein sequence

MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQEMLFSPPID

Homology

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BLAST of Moc06g38120 vs. NCBI nr
Match: XP_022156147.1 (probable E3 ubiquitin-protein ligase RHB1A isoform X1 [Momordica charantia])

HSP 1 Score: 439.1 bits (1128), Expect = 2.1e-119
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           HHFHLACILEWMERSDICPVCDQEMLFSPPID
Sbjct: 181 HHFHLACILEWMERSDICPVCDQEMLFSPPID 212

[Close]

BLAST of Moc06g38120 vs. NCBI nr
Match: XP_038876112.1 (probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida] >XP_038876113.1 probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida] >XP_038876115.1 probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida])

HSP 1 Score: 397.5 bits (1020), Expect = 7.2e-107
Identity = 190/212 (89.62%), Postives = 196/212 (92.45%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGGCCCCSSK TESNIAPSYYYYPRAS+EHVPLSSP+  P AFS GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKRTESNIAPSYYYYPRASEEHVPLSSPLSTPPAFSRGLLVDTNLDTSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           YRSPPLPMPYDVVLT P TPPVAQEIGSNKNDAAAQTTN D +QE AC N RETSAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTSPLTPPVAQEIGSNKNDAAAQTTNPDTIQETACINTRETSAKCEG 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           +DESDCKKHTDFE DTLKESEIELSKGVES+ L IEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 VDESDCKKHTDFEVDTLKESEIELSKGVESMVLPIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           HHFHLACILEWMERSDICPVCDQEM+FS PID
Sbjct: 181 HHFHLACILEWMERSDICPVCDQEMVFSSPID 212

[Close]

BLAST of Moc06g38120 vs. NCBI nr
Match: XP_022970099.1 (probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima])

HSP 1 Score: 395.2 bits (1014), Expect = 3.6e-106
Identity = 190/213 (89.20%), Postives = 197/213 (92.49%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGGCCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLNAPPAFPMGLLVDTNLDTSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           YRSPPLPMPY+VVL+HPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCEG
Sbjct: 61  YRSPPLPMPYNVVLSHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCEG 120

Query: 121 I-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKC 180
           I DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTKC
Sbjct: 121 IDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTKC 180

Query: 181 EHHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           EHHFHLACILEWMERSDICPVCDQEM+FSPPID
Sbjct: 181 EHHFHLACILEWMERSDICPVCDQEMVFSPPID 213

[Close]

BLAST of Moc06g38120 vs. NCBI nr
Match: KAG6579518.1 (putative E3 ubiquitin-protein ligase RHB1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 394.0 bits (1011), Expect = 7.9e-106
Identity = 192/214 (89.72%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MGG-CCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPD 60
           MGG CCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPD
Sbjct: 1   MGGCCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLSAPPAFPMGLLVDTNLDTSIPD 60

Query: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCE 120
           TYRSPPLPMPYDVVLTHPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCE
Sbjct: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCE 120

Query: 121 GI-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTK 180
           GI DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTK
Sbjct: 121 GIDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTK 180

Query: 181 CEHHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           CEHHFHLACILEWMERSDICPVCDQEM+FSPPID
Sbjct: 181 CEHHFHLACILEWMERSDICPVCDQEMVFSPPID 214

[Close]

BLAST of Moc06g38120 vs. NCBI nr
Match: XP_023551494.1 (probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.0 bits (1011), Expect = 7.9e-106
Identity = 192/214 (89.72%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MGG-CCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPD 60
           MGG CCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPD
Sbjct: 1   MGGCCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLSAPPAFPMGLLVDTNLDTSIPD 60

Query: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCE 120
           TYRSPPLPMPYDVVLTHPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCE
Sbjct: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCE 120

Query: 121 GI-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTK 180
           GI DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTK
Sbjct: 121 GIGDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTK 180

Query: 181 CEHHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           CEHHFHLACILEWMERSDICPVCDQEM+FSPPID
Sbjct: 181 CEHHFHLACILEWMERSDICPVCDQEMVFSPPID 214

[Close]

BLAST of Moc06g38120 vs. ExPASy Swiss-Prot
Match: Q2HIJ8 (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.6e-40
Identity = 92/207 (44.44%), Postives = 128/207 (61.84%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEMLF 208
           H FHL+C+LEW+ERSD CP+CD+E++F
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDKEVVF 185

[Close]

BLAST of Moc06g38120 vs. ExPASy Swiss-Prot
Match: Q8LE94 (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 5.3e-20
Identity = 60/180 (33.33%), Postives = 96/180 (53.33%), Query Frame = 0


Query: 33  LSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKND 92
           L S + A ++ +S     ++ D  + + +RS P P+PYD    +  +    +E GS+ + 
Sbjct: 53  LPSSVQATASITS----SSSHDNFLSEAFRSTPRPLPYDADPRYFRSLVSRREKGSSHSH 112

Query: 93  AAAQ--TTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETD--TLKESEIELSKGV 152
              +   ++SDA  E       + +     + + D K+    ++    L+     +    
Sbjct: 113 EEVEPLRSDSDADSESFGVGGCKWANNKSTLSDKDSKEEYSSKSSLRILRSRSKSIMADS 172

Query: 153 ESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQEMLFS 209
           E++ +  E+EDVCP CLEEY  ENPK+ TKC HHFHL+CI EWMERS+ CPVC + M F+
Sbjct: 173 ENMYILSEDEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVMEFN 228

[Close]

BLAST of Moc06g38120 vs. ExPASy Swiss-Prot
Match: Q93ZF6 (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.2e-18
Identity = 71/208 (34.13%), Postives = 90/208 (43.27%), Query Frame = 0


Query: 3   GCCCCSSKGTESNIAPSYYYYPRASDEHVPLS--SPIGAPSAFSSGLLVDTNLDTSIPDT 62
           GCCCC     ES+         R  DEH+PLS  +P    +A+SS L             
Sbjct: 2   GCCCCLPSIPESS---------RTIDEHLPLSRATPSSLSNAYSSPL------------- 61

Query: 63  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 122
             SPP+P+    +      P + +  G                      N+ E S     
Sbjct: 62  --SPPIPLAITNINLQTSPPKLPRTQG----------------------NSSEASPGLTQ 121

Query: 123 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 182
           +       H D  TD       EL K         E  D CPICLEEY+ +NPKL TKC 
Sbjct: 122 VVPEKKTWHVDDLTD------FELKKQYR------EAIDECPICLEEYEIDNPKLLTKCG 151

Query: 183 HHFHLACILEWMERSDICPVCDQEMLFS 209
           H FHLACIL WMERS+ CPVCD+E++ +
Sbjct: 182 HDFHLACILAWMERSEACPVCDKELVLT 151

[Close]

BLAST of Moc06g38120 vs. ExPASy Swiss-Prot
Match: Q4TU14 (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.5e-09
Identity = 23/53 (43.40%), Postives = 33/53 (62.26%), Query Frame = 0


Query: 158 EDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQEMLFSPP 211
           +D C ICLE +  ++P   T C+H +HL CI+EW +RS  CP+C Q  +   P
Sbjct: 43  DDACSICLEPFTLQDPSTVTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDP 95

[Close]

BLAST of Moc06g38120 vs. ExPASy Swiss-Prot
Match: Q9ZT42 (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.5e-09
Identity = 29/73 (39.73%), Postives = 40/73 (54.79%), Query Frame = 0


Query: 140 SEIELSKGVESVALAIEE--EDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDI 199
           SE  L+     V   I++  +D C ICLE +   +P   T C+H +HL CILEW +RS  
Sbjct: 10  SEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQ 69

Query: 200 CPVCDQEMLFSPP 211
           CP+C Q +    P
Sbjct: 70  CPMCWQSISLKDP 82

[Close]

BLAST of Moc06g38120 vs. ExPASy TrEMBL
Match: A0A6J1DR88 (probable E3 ubiquitin-protein ligase RHB1A isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023100 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.0e-119
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           HHFHLACILEWMERSDICPVCDQEMLFSPPID
Sbjct: 181 HHFHLACILEWMERSDICPVCDQEMLFSPPID 212

[Close]

BLAST of Moc06g38120 vs. ExPASy TrEMBL
Match: A0A6J1I4I7 (probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita maxima OX=3661 GN=LOC111469091 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.7e-106
Identity = 190/213 (89.20%), Postives = 197/213 (92.49%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGGCCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLNAPPAFPMGLLVDTNLDTSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           YRSPPLPMPY+VVL+HPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCEG
Sbjct: 61  YRSPPLPMPYNVVLSHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCEG 120

Query: 121 I-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKC 180
           I DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTKC
Sbjct: 121 IDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTKC 180

Query: 181 EHHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           EHHFHLACILEWMERSDICPVCDQEM+FSPPID
Sbjct: 181 EHHFHLACILEWMERSDICPVCDQEMVFSPPID 213

[Close]

BLAST of Moc06g38120 vs. ExPASy TrEMBL
Match: A0A6J1ER94 (probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita moschata OX=3662 GN=LOC111435815 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-105
Identity = 191/214 (89.25%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MGG-CCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPD 60
           MGG CCCCSSK +ESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPD
Sbjct: 1   MGGCCCCCSSKESESNIAPGYYYYPRASEEHVPLSSPLSAPPAFPMGLLVDTNLDTSIPD 60

Query: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCE 120
           TYRSPPLPMPYDVVLTHPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCE
Sbjct: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCE 120

Query: 121 GI-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTK 180
           GI DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTK
Sbjct: 121 GIDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTK 180

Query: 181 CEHHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           CEHHFHLACILEWMERSDICPVCDQEM+FSPPID
Sbjct: 181 CEHHFHLACILEWMERSDICPVCDQEMVFSPPID 214

[Close]

BLAST of Moc06g38120 vs. ExPASy TrEMBL
Match: A0A6J1DPI0 (probable E3 ubiquitin-protein ligase RHB1A isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023100 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 7.2e-105
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 23  YPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPV 82
           YPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPV
Sbjct: 3   YPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPV 62

Query: 83  AQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETDTLKESEI 142
           AQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETDTLKESEI
Sbjct: 63  AQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETDTLKESEI 122

Query: 143 ELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCD 202
           ELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCD
Sbjct: 123 ELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCD 182

Query: 203 QEMLFSPPID 213
           QEMLFSPPID
Sbjct: 183 QEMLFSPPID 192

[Close]

BLAST of Moc06g38120 vs. ExPASy TrEMBL
Match: A0A5A7TH60 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002410 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.1e-104
Identity = 185/212 (87.26%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGGCCCCSSKGTESNIAP YYYYPRAS+EHVPLSSP+  P AFS+GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKGTESNIAPGYYYYPRASEEHVPLSSPLRTPRAFSTGLLVDTNLDTSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           YRSPPLPMPYDVVLT P TPPVAQEIGS KN+AAAQT NS+ +QE AC N RE SAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTSPLTPPVAQEIGSYKNEAAAQTANSNTIQETACINTREASAKCEG 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           +DESDCKKHTDFE +TLKESEIELSKGVES  L IEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 VDESDCKKHTDFEVNTLKESEIELSKGVESAVLPIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEMLFSPPID 213
           HHFHLACILEWMERSDICPVCDQEM+FS PID
Sbjct: 181 HHFHLACILEWMERSDICPVCDQEMVFSSPID 212

[Close]

BLAST of Moc06g38120 vs. TAIR 10
Match: AT5G41350.1 (RING/U-box superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 1.6e-48
Identity = 104/216 (48.15%), Postives = 141/216 (65.28%), Query Frame = 0


Query: 1   MGGCCCC-SSKGTESNIAPSYYYYPRASDEHVPLSSPIG-APSAFSSG-LLVDTNLDTSI 60
           MGGCCCC SS+  + +  P+YYYYPRA++E VPLSS      SA S+G ++VDTNL+TS 
Sbjct: 1   MGGCCCCSSSRRADVDNGPAYYYYPRATEERVPLSSAHNRTSSAISTGVVVVDTNLETSS 60

Query: 61  PDTYRSPPLPMPYDVVLTHPHTPPVAQEIGS-NKNDAAAQTTNSDAVQEIACTNARETSA 120
           PD Y  PPLP P+DV +  P TP   +E    +  + +  + N+++ QE           
Sbjct: 61  PDAYIPPPLPTPFDVPIGIPQTPASGEEATCVDIREVSVDSANTESAQETVDGITLGVPT 120

Query: 121 KCEGIDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLT 180
            C    E+D K  T+ + ++ +E + +LSK   +V + IEEE+ CPICLEEYD ENPKL 
Sbjct: 121 TCSH-KETDSKIQTEIDLESTEEIDPKLSK---AVFIPIEEEEDCPICLEEYDIENPKLV 180

Query: 181 TKCEHHFHLACILEWMERSDICPVCDQEMLFSPPID 213
            KC+HHFHLACILEWMERS+ CPVC++EM+F   +D
Sbjct: 181 AKCDHHFHLACILEWMERSETCPVCNKEMVFDSTLD 212

[Close]

BLAST of Moc06g38120 vs. TAIR 10
Match: AT4G00335.1 (RING-H2 finger B1A )

HSP 1 Score: 167.5 bits (423), Expect = 1.1e-41
Identity = 92/207 (44.44%), Postives = 128/207 (61.84%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEMLF 208
           H FHL+C+LEW+ERSD CP+CD+E++F
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDKEVVF 185

[Close]

BLAST of Moc06g38120 vs. TAIR 10
Match: AT4G00335.2 (RING-H2 finger B1A )

HSP 1 Score: 167.5 bits (423), Expect = 1.1e-41
Identity = 92/207 (44.44%), Postives = 128/207 (61.84%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEMLF 208
           H FHL+C+LEW+ERSD CP+CD+E++F
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDKEVVF 185

[Close]

BLAST of Moc06g38120 vs. TAIR 10
Match: AT4G00335.3 (RING-H2 finger B1A )

HSP 1 Score: 162.9 bits (411), Expect = 2.8e-40
Identity = 91/206 (44.17%), Postives = 125/206 (60.68%), Query Frame = 0


Query: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 181 HHFHLACILEWMERSDICPVCDQEML 207
           H FHL+C+LEW+ERSD CP+CD+  L
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDKVKL 184

[Close]

BLAST of Moc06g38120 vs. TAIR 10
Match: AT3G02290.1 (RING/U-box superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.8e-21
Identity = 60/180 (33.33%), Postives = 96/180 (53.33%), Query Frame = 0


Query: 33  LSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKND 92
           L S + A ++ +S     ++ D  + + +RS P P+PYD    +  +    +E GS+ + 
Sbjct: 53  LPSSVQATASITS----SSSHDNFLSEAFRSTPRPLPYDADPRYFRSLVSRREKGSSHSH 112

Query: 93  AAAQ--TTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETD--TLKESEIELSKGV 152
              +   ++SDA  E       + +     + + D K+    ++    L+     +    
Sbjct: 113 EEVEPLRSDSDADSESFGVGGCKWANNKSTLSDKDSKEEYSSKSSLRILRSRSKSIMADS 172

Query: 153 ESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQEMLFS 209
           E++ +  E+EDVCP CLEEY  ENPK+ TKC HHFHL+CI EWMERS+ CPVC + M F+
Sbjct: 173 ENMYILSEDEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVMEFN 228

The following BLAST results are available for this feature:

BLAST of Moc06g38120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156147.1	2.1e-119	100.00	probable E3 ubiquitin-protein ligase RHB1A isoform X1 [Momordica charantia]	[more]
XP_038876112.1	7.2e-107	89.62	probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida] >XP_038876113.1 p...	[more]
XP_022970099.1	3.6e-106	89.20	probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima]	[more]
KAG6579518.1	7.9e-106	89.72	putative E3 ubiquitin-protein ligase RHB1A, partial [Cucurbita argyrosperma subs...	[more]
XP_023551494.1	7.9e-106	89.72	probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc06g38120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2HIJ8	1.6e-40	44.44	Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q8LE94	5.3e-20	33.33	E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022...	[more]
Q93ZF6	2.2e-18	34.13	E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ...	[more]
Q4TU14	2.5e-09	43.40	E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ...	[more]
Q9ZT42	2.5e-09	39.73	E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ...	[more]

BLAST of Moc06g38120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DR88	1.0e-119	100.00	probable E3 ubiquitin-protein ligase RHB1A isoform X1 OS=Momordica charantia OX=...	[more]
A0A6J1I4I7	1.7e-106	89.20	probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1ER94	1.1e-105	89.25	probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1DPI0	7.2e-105	100.00	probable E3 ubiquitin-protein ligase RHB1A isoform X2 OS=Momordica charantia OX=...	[more]
A0A5A7TH60	2.1e-104	87.26	E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]

BLAST of Moc06g38120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41350.1	1.6e-48	48.15	RING/U-box superfamily protein	[more]
AT4G00335.1	1.1e-41	44.44	RING-H2 finger B1A	[more]
AT4G00335.2	1.1e-41	44.44	RING-H2 finger B1A	[more]
AT4G00335.3	2.8e-40	44.17	RING-H2 finger B1A	[more]
AT3G02290.1	3.8e-21	33.33	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 161..201 e-value: 6.3E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 160..201 e-value: 1.3E-11 score: 44.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 161..202 score: 12.061441
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 133..206 e-value: 3.4E-16 score: 60.2
None	No IPR available	PANTHER	PTHR46463	ZINC FINGER, RING/FYVE/PHD-TYPE	coord: 1..212
None	No IPR available	PANTHER	PTHR46463:SF37	ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 RING-H2 FINGER PROTEIN	coord: 1..212
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 153..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g38120.1	Moc06g38120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0008270	zinc ion binding