Moc06g37850 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g37850
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionWAT1-related protein
Locationchr6: 29128689 .. 29131022 (-)
RNA-Seq ExpressionMoc06g37850
SyntenyMoc06g37850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTTGGAGAATTTGAAGCCGTTCATGGCGATGGTGGGTGTAGATTTTGGGTATGCCGTAGTGAATATTCTGATGGAGGCAGCTCTTAGCCATGGAATGAACCACTTGACTCTCATCACTTATCGCCTCTGCATCGCATCCATTTCCTTGGGCCCCATCGCATACTTTTATGAAAGGTGGTAATTTTTACTTCATTTTCCCTCAATATAGTTCATCTTCTCTTCTTCTTCTTTTTATTTGAATTATCATTTAAATTTTGTTGTGTAGGGATGGTCGAGGCAAACTCTCCTTTCGTATCCTCTCTTATTTGTTCTTAAGCTCCATTCTTGGGTCAGTTCTAAGTTTGAGAATTTTGAGAATAATTAAGAATTGATCATTGAGAATGGATAATTAATTAGTTTTTATTGTGTGGTTCTACTTGTAGGGCTTCGCTCACCCAATACTGCTTTCTTCTGGGCATCGAACACACCTCCGCTACTTTCGCCTGTGCATTCCTCAACATGATCCCTGTCGCCACTTTTCTCATGGCTCTTCTCTTTAGGTATTTCCATTCTCATTTGTAATAATATTATATTATTATGTTCTTAACTCTTTAAAGATGAGAAGGAATCTTGTCTCCAAGAAATACAAAAACAGACTTTTAAATAAAATGAGTTGTGTTGGGTTGTCAAAGGAGAATGCAAACATAAAGTCCCGCGGAAAAGAAAATGATTAGGACAATTATCTCTTGGTATGGGATATTTTGAGTGAAATCAAACAAGTATCTCCATTGATACGAGATATTTTGGGTGAAACCAAACGTAAAGTTGTGAAGCTTATGCCCAAAACGAATAATATCATATCATTATGACGATATTGTTATGGTTGCTCGACGGCACTTTTTCGCCTTTCTCTTCACTCTCGAACGGGAGGGTTAGTAGACACAGGGAGAGGGAGCAGGTAAAGCGTGTGTCGTTCGTAAATAGAAAGAAAGAGTCACTGCACTTCCCTTCTTAACAGTAGGCATCTTTCCAGGAAGTTGATGGGCTCACCATCGAATTAGGTCGGAGTGATTGGTGGTTTCGGGATCGTGTAGAAAATGCAATGAGCTTAAGTATTATCCTTTACTTTTCCCGAGTGCAGGTTGGAGAAGGTGAAGTTGAAATCCAACAGTGGAAAAGCCAAGATGCTGGGAACCGTGATTTGCATCTCCGGCGCGGTGCTGTTGATCACGTTCAAAGGACCAGCTTTGGCCAACGGTTCATACGTCGCCAAGAAAGCTGCCGCCGCCGCGGTGAAGAAGACGAATCAGACGTTGGGGTGCATAGCTCTGGTGGTCGGAACTCTACTGTGGTCGTCATGGTTTCTCCTGCAGTCAAAGATCGGGAAGAGATATCCGTACGAGTACTCCAGCACCGCCATCATGTCCTTCTTCGGTGCCGCCCAATCCGCCGTCTTCAGCCTCTCCACGGGCGCCACTCTTTCCTCTTGGGCCCTCCATGGCAACATTCAAATCATCACCGTTGTGTACTCTGTGAGTACTCTGTGAACACTAATTAATTAAAATAGATTAACCGTAATCAATTAAACAATCTATCGACTTAAAATTTATCTAGAAGTGTGAAAATTTGATTAATTTCGTGGGAATTAATTAAATGGCAGGGGATGATCGGATCGGGGCTGTGCGTGGTGGGAACCTCATGGTGCGTTAAAAAGAGAGGTCCAGTTTTCACTGCAGCCTTCAACCCCTTGGTTCAGATAATTGCAGGCATGTTCGACATCCCCTTCTTCCACGACCCACTCTATCTCGGGAAGTAAGTATTCTTTTGGTAACGTTTTCGTTTCCTATTTCTGTTTCTTCATTTTTAATAAAGAATAATAAAATTGTATTATCCCAACATCCATAAATTCAAGGATGATCAAACAATATTTAGATTTATGTTTTGAACTTGTTTTAATCGTTTCCAAAATTTCTATACAATTTTGATACTGTTTCCAACAATAAACATAACCAGTTATCAAATGCTTAAGTTTTTTATATAAAATTAAAAACAAGAAACAAGAAACAAAGAAACAGTAACGCGTTATCAAACTTACCCTAATTAACTAACTATAACCAACTAACTCCTTAATTAATTTCATAATTATTTTGTCTAGTAACACAATCAATTACATTACAACTACAGCGTGTTGGGGTCGCTGATGGTAATAATTGGACTCTACATTTTGCTGTGGGGAAAGAGCCAGGAAATGGAGGGCTCTGTTTCGAAGGTGGTGGCCATGGACCAAATTACAACTACAGCTGCTGAAGATTGTGAGATCAAAGAATTTAAACATGATGATAATCCATAG

mRNA sequence

ATGGACTTGGAGAATTTGAAGCCGTTCATGGCGATGGTGGGTGTAGATTTTGGGTATGCCGTAGTGAATATTCTGATGGAGGCAGCTCTTAGCCATGGAATGAACCACTTGACTCTCATCACTTATCGCCTCTGCATCGCATCCATTTCCTTGGGCCCCATCGCATACTTTTATGAAAGGTGGGATGGTCGAGGCAAACTCTCCTTTCGTATCCTCTCTTATTTGTTCTTAAGCTCCATTCTTGGGGCTTCGCTCACCCAATACTGCTTTCTTCTGGGCATCGAACACACCTCCGCTACTTTCGCCTGTGCATTCCTCAACATGATCCCTGTCGCCACTTTTCTCATGGCTCTTCTCTTTAGGTTGGAGAAGGTGAAGTTGAAATCCAACAGTGGAAAAGCCAAGATGCTGGGAACCGTGATTTGCATCTCCGGCGCGGTGCTGTTGATCACGTTCAAAGGACCAGCTTTGGCCAACGGTTCATACGTCGCCAAGAAAGCTGCCGCCGCCGCGGTGAAGAAGACGAATCAGACGTTGGGGTGCATAGCTCTGGTGGTCGGAACTCTACTGTGGTCGTCATGGTTTCTCCTGCAGTCAAAGATCGGGAAGAGATATCCGTACGAGTACTCCAGCACCGCCATCATGTCCTTCTTCGGTGCCGCCCAATCCGCCGTCTTCAGCCTCTCCACGGGCGCCACTCTTTCCTCTTGGGCCCTCCATGGCAACATTCAAATCATCACCGTTGTGTACTCTGGGATGATCGGATCGGGGCTGTGCGTGGTGGGAACCTCATGGTGCGTTAAAAAGAGAGGTCCAGTTTTCACTGCAGCCTTCAACCCCTTGGTTCAGATAATTGCAGGCATGTTCGACATCCCCTTCTTCCACGACCCACTCTATCTCGGGAACGTGTTGGGGTCGCTGATGGTAATAATTGGACTCTACATTTTGCTGTGGGGAAAGAGCCAGGAAATGGAGGGCTCTGTTTCGAAGGTGGTGGCCATGGACCAAATTACAACTACAGCTGCTGAAGATTGTGAGATCAAAGAATTTAAACATGATGATAATCCATAG

Coding sequence (CDS)

ATGGACTTGGAGAATTTGAAGCCGTTCATGGCGATGGTGGGTGTAGATTTTGGGTATGCCGTAGTGAATATTCTGATGGAGGCAGCTCTTAGCCATGGAATGAACCACTTGACTCTCATCACTTATCGCCTCTGCATCGCATCCATTTCCTTGGGCCCCATCGCATACTTTTATGAAAGGTGGGATGGTCGAGGCAAACTCTCCTTTCGTATCCTCTCTTATTTGTTCTTAAGCTCCATTCTTGGGGCTTCGCTCACCCAATACTGCTTTCTTCTGGGCATCGAACACACCTCCGCTACTTTCGCCTGTGCATTCCTCAACATGATCCCTGTCGCCACTTTTCTCATGGCTCTTCTCTTTAGGTTGGAGAAGGTGAAGTTGAAATCCAACAGTGGAAAAGCCAAGATGCTGGGAACCGTGATTTGCATCTCCGGCGCGGTGCTGTTGATCACGTTCAAAGGACCAGCTTTGGCCAACGGTTCATACGTCGCCAAGAAAGCTGCCGCCGCCGCGGTGAAGAAGACGAATCAGACGTTGGGGTGCATAGCTCTGGTGGTCGGAACTCTACTGTGGTCGTCATGGTTTCTCCTGCAGTCAAAGATCGGGAAGAGATATCCGTACGAGTACTCCAGCACCGCCATCATGTCCTTCTTCGGTGCCGCCCAATCCGCCGTCTTCAGCCTCTCCACGGGCGCCACTCTTTCCTCTTGGGCCCTCCATGGCAACATTCAAATCATCACCGTTGTGTACTCTGGGATGATCGGATCGGGGCTGTGCGTGGTGGGAACCTCATGGTGCGTTAAAAAGAGAGGTCCAGTTTTCACTGCAGCCTTCAACCCCTTGGTTCAGATAATTGCAGGCATGTTCGACATCCCCTTCTTCCACGACCCACTCTATCTCGGGAACGTGTTGGGGTCGCTGATGGTAATAATTGGACTCTACATTTTGCTGTGGGGAAAGAGCCAGGAAATGGAGGGCTCTGTTTCGAAGGTGGTGGCCATGGACCAAATTACAACTACAGCTGCTGAAGATTGTGAGATCAAAGAATTTAAACATGATGATAATCCATAG

Protein sequence

MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWDGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRLEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLGCIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP
Homology
BLAST of Moc06g37850 vs. NCBI nr
Match: XP_022158422.1 (WAT1-related protein At3g30340-like [Momordica charantia])

HSP 1 Score: 682.6 bits (1760), Expect = 1.9e-192
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0

Query: 1   MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER 60
           MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER
Sbjct: 1   MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER 60

Query: 61  WDGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF 120
            DGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF
Sbjct: 61  -DGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF 120

Query: 121 RLEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLG 180
           RLEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLG
Sbjct: 121 RLEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLG 180

Query: 181 CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH 240
           CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH
Sbjct: 181 CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH 240

Query: 241 GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL 300
           GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL
Sbjct: 241 GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL 300

Query: 301 GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP 357
           GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP
Sbjct: 301 GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP 355

BLAST of Moc06g37850 vs. NCBI nr
Match: XP_022157110.1 (WAT1-related protein At3g30340-like [Momordica charantia])

HSP 1 Score: 564.3 bits (1453), Expect = 7.4e-157
Identity = 296/345 (85.80%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 12  MVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWDGRGKLSFRI 71
           MVGVDFGYA+VNILMEAALS GMNHLTLITYRL IASISLGPIAYFYER DGR KLSFRI
Sbjct: 1   MVGVDFGYAIVNILMEAALSDGMNHLTLITYRLGIASISLGPIAYFYER-DGRDKLSFRI 60

Query: 72  LSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRLEKVKLKSNS 131
           LSYLFLS+I+GAS+TQYC LLGIEHTSATFACAFLN+IPVATFLMALL RLE VKLKS+S
Sbjct: 61  LSYLFLSAIVGASVTQYCLLLGIEHTSATFACAFLNLIPVATFLMALLLRLETVKLKSSS 120

Query: 132 GKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLGCIALVVGTLLW 191
           G+AKMLGTV+CISGAVLLIT+KGPALAN SY A  AAA AVKKTN TLGCIALVVGTLLW
Sbjct: 121 GQAKMLGTVVCISGAVLLITYKGPALANRSYAA--AAATAVKKTNWTLGCIALVVGTLLW 180

Query: 192 SSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGNIQIITVVYS 251
           SSWFLLQ+ IGKRYPYEYSSTAIMSFFGAAQ+AVF+L T  TLSSW LHG IQIITVVYS
Sbjct: 181 SSWFLLQTNIGKRYPYEYSSTAIMSFFGAAQAAVFNLCTRTTLSSWGLHGKIQIITVVYS 240

Query: 252 GMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGNVLGSLMVII 311
           GMIGSGLCVVGTSWCVKKRGPVFTAAF+PLVQIIA +FDIP  HDPLYLGN+LGSL+VII
Sbjct: 241 GMIGSGLCVVGTSWCVKKRGPVFTAAFSPLVQIIAAVFDIPVLHDPLYLGNLLGSLIVII 300

Query: 312 GLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP 357
           GLY+LLWGKS+E+EGSVSKVVAM+QITTT A+D  IKE  H DNP
Sbjct: 301 GLYVLLWGKSKEVEGSVSKVVAMEQITTTTAQDYPIKELNHHDNP 342

BLAST of Moc06g37850 vs. NCBI nr
Match: XP_022157115.1 (WAT1-related protein At3g30340-like isoform X1 [Momordica charantia])

HSP 1 Score: 508.4 bits (1308), Expect = 4.8e-140
Identity = 264/347 (76.08%), Postives = 296/347 (85.30%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           ++++KP MAMVGVD G+A+V ILME  ++ GMNHL LITYRL IA+ISL PIAYFYER D
Sbjct: 1   MKDMKPLMAMVGVDLGFAIVTILMETVINDGMNHLVLITYRLGIAAISLAPIAYFYER-D 60

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
           GR KLSFRIL YL+LSSI+GASLTQYCFLLGIEHTSA FACAFLNMIPV TF+MALLFR+
Sbjct: 61  GRAKLSFRILCYLYLSSIIGASLTQYCFLLGIEHTSAAFACAFLNMIPVVTFIMALLFRM 120

Query: 123 EKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLGCI 182
           E V+L SNSGKAK LGTV+CISG  LLI +KGPALAN SY AKKAAA AVKKT+ TLGCI
Sbjct: 121 ETVELNSNSGKAKTLGTVVCISGVALLIAYKGPALANSSYTAKKAAATAVKKTSWTLGCI 180

Query: 183 ALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGN 242
           ALV GTLLWSSW+LLQS IGKRYPYEY+STAIMSFF A QSAVF L T ATLSSW L G+
Sbjct: 181 ALVGGTLLWSSWYLLQSDIGKRYPYEYTSTAIMSFFAATQSAVFGLLTDATLSSWTLRGS 240

Query: 243 IQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGN 302
           IQI+TV+YSGMIGSGLCVVG SWCVKKRGPVFTAAFNPLVQIIA +F IP FHDPLYLGN
Sbjct: 241 IQILTVLYSGMIGSGLCVVGMSWCVKKRGPVFTAAFNPLVQIIAAVFSIPVFHDPLYLGN 300

Query: 303 VLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKE 350
           +LGS + IIGLYILLWGK  EME   S+VV M+   +T A+  E+++
Sbjct: 301 LLGSAIAIIGLYILLWGK--EMERCPSQVVVMEGSVSTTAQTMELQD 344

BLAST of Moc06g37850 vs. NCBI nr
Match: XP_022155614.1 (WAT1-related protein At3g30340-like isoform X1 [Momordica charantia])

HSP 1 Score: 507.7 bits (1306), Expect = 8.3e-140
Identity = 261/350 (74.57%), Postives = 298/350 (85.14%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           +E+LKP +AMV VD G+A+VNIL+E  L HGMNHL LITYRL IA+ISL PIAYFYER D
Sbjct: 6   VEDLKPLIAMVAVDLGFAIVNILLETVLDHGMNHLVLITYRLAIAAISLAPIAYFYER-D 65

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
           GR KLSFRIL YLF S+I+GASLTQYCFLLGI+HTSATFACAF+N++PVATFL+AL F L
Sbjct: 66  GRNKLSFRILCYLFFSAIVGASLTQYCFLLGIQHTSATFACAFINIVPVATFLLALPFGL 125

Query: 123 EKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAV--------KK 182
           E + LK +SGKAKM+GTV+CISGAVLL  +KGPALANGSY  KK AAAA         + 
Sbjct: 126 ETLNLKCSSGKAKMVGTVVCISGAVLLTAYKGPALANGSYAEKKVAAAAAAHGGAKKPRA 185

Query: 183 TNQTLGCIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATL 242
            + T+GCIALV GTLLWSSWF LQS IGKRYPY+Y STAIMSFFGAAQSAV SL+TGATL
Sbjct: 186 ESWTVGCIALVAGTLLWSSWFPLQSNIGKRYPYQYWSTAIMSFFGAAQSAVLSLATGATL 245

Query: 243 SSWALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFF 302
           SSWAL GNI+I+TVVYSGMIGSGLCVVG SWCVKKRGPVFTAAF+PLVQI+AGMFDIP  
Sbjct: 246 SSWALDGNIKILTVVYSGMIGSGLCVVGMSWCVKKRGPVFTAAFSPLVQIMAGMFDIPIL 305

Query: 303 HDPLYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAED 345
           HDPLYLGN+LGS++VI+GLYILLWGK++EM+   SKVV M+ I T  A+D
Sbjct: 306 HDPLYLGNLLGSVIVIVGLYILLWGKNKEMKNCASKVVVMEGIVTATAQD 354

BLAST of Moc06g37850 vs. NCBI nr
Match: XP_004145962.2 (WAT1-related protein At3g30340 [Cucumis sativus] >KGN49962.1 hypothetical protein Csa_000218 [Cucumis sativus])

HSP 1 Score: 483.8 bits (1244), Expect = 1.3e-132
Identity = 247/340 (72.65%), Postives = 289/340 (85.00%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           LE+ KP MAMV +DF +A+VNIL+E  L HGMNHL LITYRL IA+ISL PIAYF E+ D
Sbjct: 9   LEDFKPLMAMVAIDFAFAIVNILLETVLDHGMNHLVLITYRLSIAAISLAPIAYFCEK-D 68

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
           GR  LS RIL YLF S+I+GASLTQYCFLLGI+HTSATFACAF+N++PV TFL+AL F L
Sbjct: 69  GRANLSLRILCYLFFSAIVGASLTQYCFLLGIQHTSATFACAFVNIVPVVTFLLALPFGL 128

Query: 123 EKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQ--TLG 182
           E VKLKS+SGKAK++GTV+CISGAVLL T+KGP LAN SYV    + A +KKT +  T+G
Sbjct: 129 ESVKLKSSSGKAKVIGTVVCISGAVLLTTYKGPTLANASYVTAHVSDAKLKKTAESWTVG 188

Query: 183 CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH 242
           CIALV GTLLWSSWFLLQS IGKRYPY+YSSTAIMS FGA QSAV SLSTGA LS+WAL 
Sbjct: 189 CIALVAGTLLWSSWFLLQSNIGKRYPYQYSSTAIMSGFGAIQSAVLSLSTGAKLSAWALT 248

Query: 243 GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL 302
           G IQI+TV+Y+GMIGSG+C VG SWCVKKRGPVFTAAF+PLVQI+A MFDIP  H+PL+L
Sbjct: 249 GKIQILTVLYTGMIGSGICFVGMSWCVKKRGPVFTAAFSPLVQIMAAMFDIPILHEPLFL 308

Query: 303 GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTT 341
           G++LGS++VIIGLYILLWGK++EME   SKVV  ++IT+T
Sbjct: 309 GSLLGSIIVIIGLYILLWGKNKEMESCASKVVVEEEITST 347

BLAST of Moc06g37850 vs. ExPASy Swiss-Prot
Match: Q9LI65 (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.0e-79
Identity = 151/340 (44.41%), Postives = 230/340 (67.65%), Query Frame = 0

Query: 2   DLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERW 61
           D +  K  + M  ++ G +VVN++ +  +  G+N +   TYRL + ++ L P A F ER 
Sbjct: 5   DTKLWKAVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLER- 64

Query: 62  DGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFR 121
             R KL+ RIL  LF S++LG SL QY FL+G+E+TS+TF+ AF NM+P  TF +AL+FR
Sbjct: 65  HNRPKLTGRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFR 124

Query: 122 LEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALAN------GSYVAKKAAAAAVKKT 181
            E + +KSN G+AK+LGT+ICI GA++L  +KG AL+        ++    +  A  +K 
Sbjct: 125 QETLNIKSNVGRAKLLGTMICICGALVLTLYKGTALSREHSTHMETHTRTDSTGAMTQK- 184

Query: 182 NQTLGCIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLS 241
              +G I LV+  ++WSSWF++Q+KI + YP +Y+ST I+SFFG  QSA+ SL +  + S
Sbjct: 185 -WAMGSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTS 244

Query: 242 SWALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFH 301
            W +    Q++ ++YSG++GSGLC VG SWC+++RG VFT++F PL+Q+ A +F   F H
Sbjct: 245 MWVVKDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLH 304

Query: 302 DPLYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMD 336
           + +Y G+V+GS+++I+GLYILLWGKS++   SV+K   +D
Sbjct: 305 EQIYCGSVIGSMVIIVGLYILLWGKSKDKSASVTKQEPLD 341

BLAST of Moc06g37850 vs. ExPASy Swiss-Prot
Match: Q9M130 (WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.1e-73
Identity = 142/326 (43.56%), Postives = 212/326 (65.03%), Query Frame = 0

Query: 8   PFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWDGRGKL 67
           P + MV ++    + N L++  L  G+NH+ + TYRL I+++ L PIA+F+ER   R  L
Sbjct: 10  PVIIMVMINSALGLANALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWER-KTRPTL 69

Query: 68  SFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRLEKVKL 127
           +  IL  LF S+++GASLTQY FLLG+ +TSAT ACAF++M P  TF+MAL+FR+EK+ +
Sbjct: 70  TLNILVQLFFSALVGASLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKLNM 129

Query: 128 KSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAA---AAVKKTNQTLGCIAL 187
           KS +G   ++G +ICI GA+LL  +KG  L     +          A+K  N  +GC+ L
Sbjct: 130 KSKAGMGMVMGALICIGGALLLTMYKGVPLTKLRKLETHQLINNNHAMKPENWIIGCVLL 189

Query: 188 VVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGNIQ 247
             G+  + SW L+Q+K+ ++YP +YSST ++SFFG  Q A+ SL     +++W L   + 
Sbjct: 190 FAGSSCFGSWMLIQAKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILTDKLD 249

Query: 248 IITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGNVL 307
           I+T+VY+G +  G+C VGTSWC++KRGP+FT+ F P+  I A +FD    H  ++LG+V+
Sbjct: 250 IVTIVYAGAVAQGICTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFLGSVV 309

Query: 308 GSLMVIIGLYILLWGKSQEMEGSVSK 331
           GS +VI GLYI L GK + M+    K
Sbjct: 310 GSGVVIFGLYIFLLGKVRLMKEECEK 334

BLAST of Moc06g37850 vs. ExPASy Swiss-Prot
Match: Q5XEZ0 (WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 142/338 (42.01%), Postives = 209/338 (61.83%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           +E   P + MV  +     VN L++ AL  G+NH+ +  YR+ I+++ L P AY  ER  
Sbjct: 11  VEKYSPVIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLER-K 70

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
            R +++FR++   F+S +LGASL Q+ FLLG+ +TSAT +CA ++M+P  TF +AL+FR 
Sbjct: 71  TRPQITFRLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRT 130

Query: 123 EKVK-LKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAA----AAVKKTNQ 182
           E VK LK+ +G  K++GT+ICISGA+ L  +KGP ++N    +   A+       K  N 
Sbjct: 131 ENVKILKTKAGMLKVIGTLICISGALFLTFYKGPQISNSHSHSHGGASHNNNDQDKANNW 190

Query: 183 TLGCIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSW 242
            LGC+ L +GT+L S W L Q  +  +YP +YSST +MS F A Q A+ SL     ++ W
Sbjct: 191 LLGCLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDW 250

Query: 243 ALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDP 302
            +     I  ++Y+G++G  +  V T+W +KK G VF +AF PL  I A +FD    H P
Sbjct: 251 IIDDRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTP 310

Query: 303 LYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMD 336
           LYLG+V+GSL+ I GLY+ LWGK++E E S +    MD
Sbjct: 311 LYLGSVIGSLVTITGLYMFLWGKNKETESSTALSSGMD 347

BLAST of Moc06g37850 vs. ExPASy Swiss-Prot
Match: Q9M129 (WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.6e-64
Identity = 136/320 (42.50%), Postives = 206/320 (64.38%), Query Frame = 0

Query: 8   PFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWDGRGKL 67
           P + ++  +    +VN L++  L  G+NH+ + TYRL I+++ L P+AYF+ER   R KL
Sbjct: 10  PMIVLIVSNMIAGMVNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWER-KTRPKL 69

Query: 68  SFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRLEKVKL 127
           +  I   LF+S++ GASL QY +LLG+ +TSAT   AF  ++P  TF+MAL+F  EK+ L
Sbjct: 70  TLSISCQLFVSALFGASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKLSL 129

Query: 128 KSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKT---NQTLGCIAL 187
           K+  G   +LGT+I + G +LL  ++G  L N    + + AA +   T   N   GC  L
Sbjct: 130 KTKIGYGVVLGTLISLVGGLLLTMYQGIPLTN----SPEQAANSNNHTGHENWIKGCFFL 189

Query: 188 VVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGNIQ 247
           + G +L+SSW L+Q+KI  +YP  YSST I+S FG  Q A+ SL     L  W L   + 
Sbjct: 190 LTGVVLFSSWMLIQAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWILRDELT 249

Query: 248 IITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGNVL 307
           IITVV +G++  G+C VG SWC+K++GPV +++F+P+V + A +FD    H  +YLG+V+
Sbjct: 250 IITVVIAGVVAQGMCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIYLGSVI 309

Query: 308 GSLMVIIGLYILLWGKSQEM 325
           GS++V+IGLYI LW +S+++
Sbjct: 310 GSVVVVIGLYIFLWSRSKQI 324

BLAST of Moc06g37850 vs. ExPASy Swiss-Prot
Match: Q9M131 (WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.2e-62
Identity = 143/364 (39.29%), Postives = 214/364 (58.79%), Query Frame = 0

Query: 1   MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER 60
           M  E   P + M+        VN L++ AL  G+NH+    YR+ I+++ L P +Y +ER
Sbjct: 2   MKEEQWAPVIVMLISSVAMGSVNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWER 61

Query: 61  WDGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF 120
              R +L+F +L   F+S +LGASL Q+ FLLG+ +TSAT + A ++M+P  TF +AL+F
Sbjct: 62  -KTRPQLTFMLLCEHFISGLLGASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIF 121

Query: 121 RLEKVK-LKSNSGKAKMLGTVICISGAVLLITFKGPALAN-GSYVAKKAAAAAVKKTNQT 180
           R+E  + LKS +G  K++GT+ICI GA+LL  +KGP L+N  S+   +         +QT
Sbjct: 122 RIENAQNLKSKAGVLKVMGTLICIMGAMLLTFYKGPELSNPHSHPQARHNNNNNNGHDQT 181

Query: 181 ----LGCIALVVGTLLWSSWFLLQSKIGKRYP-YEYSSTAIMSFFGAAQSAVFSLSTGAT 240
               LGC+ LV+GT+L S W L Q K+  +YP  +YSST +MS F + Q A+ SL     
Sbjct: 182 KKWLLGCLYLVIGTVLLSLWMLFQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRD 241

Query: 241 LSSWALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPF 300
           +  W +     I+  +Y+G++G  +  V TSW +K  G VF + F+P+  + A +FD   
Sbjct: 242 VKDWIIEDKFVILVTLYAGIVGQAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLI 301

Query: 301 FHDPLYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTA-AEDCEIKEFKH 357
            H PLYLG++LGS++ I GLY+ LWG+  E + SVSK +   Q +     ED  I   K 
Sbjct: 302 LHSPLYLGSILGSVVTITGLYVFLWGRKNETDQSVSKTLNSSQFSQNKDNEDHTIANHKD 361

BLAST of Moc06g37850 vs. ExPASy TrEMBL
Match: A0A6J1DX69 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111024913 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 9.1e-193
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0

Query: 1   MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER 60
           MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER
Sbjct: 1   MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER 60

Query: 61  WDGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF 120
            DGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF
Sbjct: 61  -DGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF 120

Query: 121 RLEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLG 180
           RLEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLG
Sbjct: 121 RLEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLG 180

Query: 181 CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH 240
           CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH
Sbjct: 181 CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH 240

Query: 241 GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL 300
           GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL
Sbjct: 241 GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL 300

Query: 301 GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP 357
           GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP
Sbjct: 301 GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP 355

BLAST of Moc06g37850 vs. ExPASy TrEMBL
Match: A0A6J1DS66 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111023912 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.6e-157
Identity = 296/345 (85.80%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 12  MVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWDGRGKLSFRI 71
           MVGVDFGYA+VNILMEAALS GMNHLTLITYRL IASISLGPIAYFYER DGR KLSFRI
Sbjct: 1   MVGVDFGYAIVNILMEAALSDGMNHLTLITYRLGIASISLGPIAYFYER-DGRDKLSFRI 60

Query: 72  LSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRLEKVKLKSNS 131
           LSYLFLS+I+GAS+TQYC LLGIEHTSATFACAFLN+IPVATFLMALL RLE VKLKS+S
Sbjct: 61  LSYLFLSAIVGASVTQYCLLLGIEHTSATFACAFLNLIPVATFLMALLLRLETVKLKSSS 120

Query: 132 GKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLGCIALVVGTLLW 191
           G+AKMLGTV+CISGAVLLIT+KGPALAN SY A  AAA AVKKTN TLGCIALVVGTLLW
Sbjct: 121 GQAKMLGTVVCISGAVLLITYKGPALANRSYAA--AAATAVKKTNWTLGCIALVVGTLLW 180

Query: 192 SSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGNIQIITVVYS 251
           SSWFLLQ+ IGKRYPYEYSSTAIMSFFGAAQ+AVF+L T  TLSSW LHG IQIITVVYS
Sbjct: 181 SSWFLLQTNIGKRYPYEYSSTAIMSFFGAAQAAVFNLCTRTTLSSWGLHGKIQIITVVYS 240

Query: 252 GMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGNVLGSLMVII 311
           GMIGSGLCVVGTSWCVKKRGPVFTAAF+PLVQIIA +FDIP  HDPLYLGN+LGSL+VII
Sbjct: 241 GMIGSGLCVVGTSWCVKKRGPVFTAAFSPLVQIIAAVFDIPVLHDPLYLGNLLGSLIVII 300

Query: 312 GLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKEFKHDDNP 357
           GLY+LLWGKS+E+EGSVSKVVAM+QITTT A+D  IKE  H DNP
Sbjct: 301 GLYVLLWGKSKEVEGSVSKVVAMEQITTTTAQDYPIKELNHHDNP 342

BLAST of Moc06g37850 vs. ExPASy TrEMBL
Match: A0A6J1DS72 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111023915 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.3e-140
Identity = 264/347 (76.08%), Postives = 296/347 (85.30%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           ++++KP MAMVGVD G+A+V ILME  ++ GMNHL LITYRL IA+ISL PIAYFYER D
Sbjct: 1   MKDMKPLMAMVGVDLGFAIVTILMETVINDGMNHLVLITYRLGIAAISLAPIAYFYER-D 60

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
           GR KLSFRIL YL+LSSI+GASLTQYCFLLGIEHTSA FACAFLNMIPV TF+MALLFR+
Sbjct: 61  GRAKLSFRILCYLYLSSIIGASLTQYCFLLGIEHTSAAFACAFLNMIPVVTFIMALLFRM 120

Query: 123 EKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQTLGCI 182
           E V+L SNSGKAK LGTV+CISG  LLI +KGPALAN SY AKKAAA AVKKT+ TLGCI
Sbjct: 121 ETVELNSNSGKAKTLGTVVCISGVALLIAYKGPALANSSYTAKKAAATAVKKTSWTLGCI 180

Query: 183 ALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGN 242
           ALV GTLLWSSW+LLQS IGKRYPYEY+STAIMSFF A QSAVF L T ATLSSW L G+
Sbjct: 181 ALVGGTLLWSSWYLLQSDIGKRYPYEYTSTAIMSFFAATQSAVFGLLTDATLSSWTLRGS 240

Query: 243 IQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGN 302
           IQI+TV+YSGMIGSGLCVVG SWCVKKRGPVFTAAFNPLVQIIA +F IP FHDPLYLGN
Sbjct: 241 IQILTVLYSGMIGSGLCVVGMSWCVKKRGPVFTAAFNPLVQIIAAVFSIPVFHDPLYLGN 300

Query: 303 VLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAEDCEIKE 350
           +LGS + IIGLYILLWGK  EME   S+VV M+   +T A+  E+++
Sbjct: 301 LLGSAIAIIGLYILLWGK--EMERCPSQVVVMEGSVSTTAQTMELQD 344

BLAST of Moc06g37850 vs. ExPASy TrEMBL
Match: A0A6J1DQS8 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111022703 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 4.0e-140
Identity = 261/350 (74.57%), Postives = 298/350 (85.14%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           +E+LKP +AMV VD G+A+VNIL+E  L HGMNHL LITYRL IA+ISL PIAYFYER D
Sbjct: 6   VEDLKPLIAMVAVDLGFAIVNILLETVLDHGMNHLVLITYRLAIAAISLAPIAYFYER-D 65

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
           GR KLSFRIL YLF S+I+GASLTQYCFLLGI+HTSATFACAF+N++PVATFL+AL F L
Sbjct: 66  GRNKLSFRILCYLFFSAIVGASLTQYCFLLGIQHTSATFACAFINIVPVATFLLALPFGL 125

Query: 123 EKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAV--------KK 182
           E + LK +SGKAKM+GTV+CISGAVLL  +KGPALANGSY  KK AAAA         + 
Sbjct: 126 ETLNLKCSSGKAKMVGTVVCISGAVLLTAYKGPALANGSYAEKKVAAAAAAHGGAKKPRA 185

Query: 183 TNQTLGCIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATL 242
            + T+GCIALV GTLLWSSWF LQS IGKRYPY+Y STAIMSFFGAAQSAV SL+TGATL
Sbjct: 186 ESWTVGCIALVAGTLLWSSWFPLQSNIGKRYPYQYWSTAIMSFFGAAQSAVLSLATGATL 245

Query: 243 SSWALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFF 302
           SSWAL GNI+I+TVVYSGMIGSGLCVVG SWCVKKRGPVFTAAF+PLVQI+AGMFDIP  
Sbjct: 246 SSWALDGNIKILTVVYSGMIGSGLCVVGMSWCVKKRGPVFTAAFSPLVQIMAGMFDIPIL 305

Query: 303 HDPLYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTAAED 345
           HDPLYLGN+LGS++VI+GLYILLWGK++EM+   SKVV M+ I T  A+D
Sbjct: 306 HDPLYLGNLLGSVIVIVGLYILLWGKNKEMKNCASKVVVMEGIVTATAQD 354

BLAST of Moc06g37850 vs. ExPASy TrEMBL
Match: A0A0A0KNK7 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G146850 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.2e-133
Identity = 247/340 (72.65%), Postives = 289/340 (85.00%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           LE+ KP MAMV +DF +A+VNIL+E  L HGMNHL LITYRL IA+ISL PIAYF E+ D
Sbjct: 9   LEDFKPLMAMVAIDFAFAIVNILLETVLDHGMNHLVLITYRLSIAAISLAPIAYFCEK-D 68

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
           GR  LS RIL YLF S+I+GASLTQYCFLLGI+HTSATFACAF+N++PV TFL+AL F L
Sbjct: 69  GRANLSLRILCYLFFSAIVGASLTQYCFLLGIQHTSATFACAFVNIVPVVTFLLALPFGL 128

Query: 123 EKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKTNQ--TLG 182
           E VKLKS+SGKAK++GTV+CISGAVLL T+KGP LAN SYV    + A +KKT +  T+G
Sbjct: 129 ESVKLKSSSGKAKVIGTVVCISGAVLLTTYKGPTLANASYVTAHVSDAKLKKTAESWTVG 188

Query: 183 CIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALH 242
           CIALV GTLLWSSWFLLQS IGKRYPY+YSSTAIMS FGA QSAV SLSTGA LS+WAL 
Sbjct: 189 CIALVAGTLLWSSWFLLQSNIGKRYPYQYSSTAIMSGFGAIQSAVLSLSTGAKLSAWALT 248

Query: 243 GNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYL 302
           G IQI+TV+Y+GMIGSG+C VG SWCVKKRGPVFTAAF+PLVQI+A MFDIP  H+PL+L
Sbjct: 249 GKIQILTVLYTGMIGSGICFVGMSWCVKKRGPVFTAAFSPLVQIMAAMFDIPILHEPLFL 308

Query: 303 GNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTT 341
           G++LGS++VIIGLYILLWGK++EME   SKVV  ++IT+T
Sbjct: 309 GSLLGSIIVIIGLYILLWGKNKEMESCASKVVVEEEITST 347

BLAST of Moc06g37850 vs. TAIR 10
Match: AT3G30340.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 298.5 bits (763), Expect = 7.1e-81
Identity = 151/340 (44.41%), Postives = 230/340 (67.65%), Query Frame = 0

Query: 2   DLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERW 61
           D +  K  + M  ++ G +VVN++ +  +  G+N +   TYRL + ++ L P A F ER 
Sbjct: 5   DTKLWKAVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLER- 64

Query: 62  DGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFR 121
             R KL+ RIL  LF S++LG SL QY FL+G+E+TS+TF+ AF NM+P  TF +AL+FR
Sbjct: 65  HNRPKLTGRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFR 124

Query: 122 LEKVKLKSNSGKAKMLGTVICISGAVLLITFKGPALAN------GSYVAKKAAAAAVKKT 181
            E + +KSN G+AK+LGT+ICI GA++L  +KG AL+        ++    +  A  +K 
Sbjct: 125 QETLNIKSNVGRAKLLGTMICICGALVLTLYKGTALSREHSTHMETHTRTDSTGAMTQK- 184

Query: 182 NQTLGCIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLS 241
              +G I LV+  ++WSSWF++Q+KI + YP +Y+ST I+SFFG  QSA+ SL +  + S
Sbjct: 185 -WAMGSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTS 244

Query: 242 SWALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFH 301
            W +    Q++ ++YSG++GSGLC VG SWC+++RG VFT++F PL+Q+ A +F   F H
Sbjct: 245 MWVVKDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLH 304

Query: 302 DPLYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMD 336
           + +Y G+V+GS+++I+GLYILLWGKS++   SV+K   +D
Sbjct: 305 EQIYCGSVIGSMVIIVGLYILLWGKSKDKSASVTKQEPLD 341

BLAST of Moc06g37850 vs. TAIR 10
Match: AT4G01440.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.2e-74
Identity = 142/326 (43.56%), Postives = 212/326 (65.03%), Query Frame = 0

Query: 8   PFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWDGRGKL 67
           P + MV ++    + N L++  L  G+NH+ + TYRL I+++ L PIA+F+ER   R  L
Sbjct: 10  PVIIMVMINSALGLANALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWER-KTRPTL 69

Query: 68  SFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRLEKVKL 127
           +  IL  LF S+++GASLTQY FLLG+ +TSAT ACAF++M P  TF+MAL+FR+EK+ +
Sbjct: 70  TLNILVQLFFSALVGASLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKLNM 129

Query: 128 KSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAA---AAVKKTNQTLGCIAL 187
           KS +G   ++G +ICI GA+LL  +KG  L     +          A+K  N  +GC+ L
Sbjct: 130 KSKAGMGMVMGALICIGGALLLTMYKGVPLTKLRKLETHQLINNNHAMKPENWIIGCVLL 189

Query: 188 VVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGNIQ 247
             G+  + SW L+Q+K+ ++YP +YSST ++SFFG  Q A+ SL     +++W L   + 
Sbjct: 190 FAGSSCFGSWMLIQAKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILTDKLD 249

Query: 248 IITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGNVL 307
           I+T+VY+G +  G+C VGTSWC++KRGP+FT+ F P+  I A +FD    H  ++LG+V+
Sbjct: 250 IVTIVYAGAVAQGICTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFLGSVV 309

Query: 308 GSLMVIIGLYILLWGKSQEMEGSVSK 331
           GS +VI GLYI L GK + M+    K
Sbjct: 310 GSGVVIFGLYIFLLGKVRLMKEECEK 334

BLAST of Moc06g37850 vs. TAIR 10
Match: AT1G01070.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 258.5 bits (659), Expect = 8.1e-69
Identity = 142/338 (42.01%), Postives = 209/338 (61.83%), Query Frame = 0

Query: 3   LENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWD 62
           +E   P + MV  +     VN L++ AL  G+NH+ +  YR+ I+++ L P AY  ER  
Sbjct: 11  VEKYSPVIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLER-K 70

Query: 63  GRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRL 122
            R +++FR++   F+S +LGASL Q+ FLLG+ +TSAT +CA ++M+P  TF +AL+FR 
Sbjct: 71  TRPQITFRLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRT 130

Query: 123 EKVK-LKSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAA----AAVKKTNQ 182
           E VK LK+ +G  K++GT+ICISGA+ L  +KGP ++N    +   A+       K  N 
Sbjct: 131 ENVKILKTKAGMLKVIGTLICISGALFLTFYKGPQISNSHSHSHGGASHNNNDQDKANNW 190

Query: 183 TLGCIALVVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSW 242
            LGC+ L +GT+L S W L Q  +  +YP +YSST +MS F A Q A+ SL     ++ W
Sbjct: 191 LLGCLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDW 250

Query: 243 ALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDP 302
            +     I  ++Y+G++G  +  V T+W +KK G VF +AF PL  I A +FD    H P
Sbjct: 251 IIDDRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTP 310

Query: 303 LYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMD 336
           LYLG+V+GSL+ I GLY+ LWGK++E E S +    MD
Sbjct: 311 LYLGSVIGSLVTITGLYMFLWGKNKETESSTALSSGMD 347

BLAST of Moc06g37850 vs. TAIR 10
Match: AT4G01450.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-65
Identity = 136/320 (42.50%), Postives = 206/320 (64.38%), Query Frame = 0

Query: 8   PFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYERWDGRGKL 67
           P + ++  +    +VN L++  L  G+NH+ + TYRL I+++ L P+AYF+ER   R KL
Sbjct: 10  PMIVLIVSNMIAGMVNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWER-KTRPKL 69

Query: 68  SFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLFRLEKVKL 127
           +  I   LF+S++ GASL QY +LLG+ +TSAT   AF  ++P  TF+MAL+F  EK+ L
Sbjct: 70  TLSISCQLFVSALFGASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKLSL 129

Query: 128 KSNSGKAKMLGTVICISGAVLLITFKGPALANGSYVAKKAAAAAVKKT---NQTLGCIAL 187
           K+  G   +LGT+I + G +LL  ++G  L N    + + AA +   T   N   GC  L
Sbjct: 130 KTKIGYGVVLGTLISLVGGLLLTMYQGIPLTN----SPEQAANSNNHTGHENWIKGCFFL 189

Query: 188 VVGTLLWSSWFLLQSKIGKRYPYEYSSTAIMSFFGAAQSAVFSLSTGATLSSWALHGNIQ 247
           + G +L+SSW L+Q+KI  +YP  YSST I+S FG  Q A+ SL     L  W L   + 
Sbjct: 190 LTGVVLFSSWMLIQAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWILRDELT 249

Query: 248 IITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPFFHDPLYLGNVL 307
           IITVV +G++  G+C VG SWC+K++GPV +++F+P+V + A +FD    H  +YLG+V+
Sbjct: 250 IITVVIAGVVAQGMCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIYLGSVI 309

Query: 308 GSLMVIIGLYILLWGKSQEM 325
           GS++V+IGLYI LW +S+++
Sbjct: 310 GSVVVVIGLYIFLWSRSKQI 324

BLAST of Moc06g37850 vs. TAIR 10
Match: AT4G01430.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 240.0 bits (611), Expect = 3.0e-63
Identity = 143/364 (39.29%), Postives = 214/364 (58.79%), Query Frame = 0

Query: 1   MDLENLKPFMAMVGVDFGYAVVNILMEAALSHGMNHLTLITYRLCIASISLGPIAYFYER 60
           M  E   P + M+        VN L++ AL  G+NH+    YR+ I+++ L P +Y +ER
Sbjct: 2   MKEEQWAPVIVMLISSVAMGSVNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWER 61

Query: 61  WDGRGKLSFRILSYLFLSSILGASLTQYCFLLGIEHTSATFACAFLNMIPVATFLMALLF 120
              R +L+F +L   F+S +LGASL Q+ FLLG+ +TSAT + A ++M+P  TF +AL+F
Sbjct: 62  -KTRPQLTFMLLCEHFISGLLGASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIF 121

Query: 121 RLEKVK-LKSNSGKAKMLGTVICISGAVLLITFKGPALAN-GSYVAKKAAAAAVKKTNQT 180
           R+E  + LKS +G  K++GT+ICI GA+LL  +KGP L+N  S+   +         +QT
Sbjct: 122 RIENAQNLKSKAGVLKVMGTLICIMGAMLLTFYKGPELSNPHSHPQARHNNNNNNGHDQT 181

Query: 181 ----LGCIALVVGTLLWSSWFLLQSKIGKRYP-YEYSSTAIMSFFGAAQSAVFSLSTGAT 240
               LGC+ LV+GT+L S W L Q K+  +YP  +YSST +MS F + Q A+ SL     
Sbjct: 182 KKWLLGCLYLVIGTVLLSLWMLFQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRD 241

Query: 241 LSSWALHGNIQIITVVYSGMIGSGLCVVGTSWCVKKRGPVFTAAFNPLVQIIAGMFDIPF 300
           +  W +     I+  +Y+G++G  +  V TSW +K  G VF + F+P+  + A +FD   
Sbjct: 242 VKDWIIEDKFVILVTLYAGIVGQAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLI 301

Query: 301 FHDPLYLGNVLGSLMVIIGLYILLWGKSQEMEGSVSKVVAMDQITTTA-AEDCEIKEFKH 357
            H PLYLG++LGS++ I GLY+ LWG+  E + SVSK +   Q +     ED  I   K 
Sbjct: 302 LHSPLYLGSILGSVVTITGLYVFLWGRKNETDQSVSKTLNSSQFSQNKDNEDHTIANHKD 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158422.11.9e-19299.72WAT1-related protein At3g30340-like [Momordica charantia][more]
XP_022157110.17.4e-15785.80WAT1-related protein At3g30340-like [Momordica charantia][more]
XP_022157115.14.8e-14076.08WAT1-related protein At3g30340-like isoform X1 [Momordica charantia][more]
XP_022155614.18.3e-14074.57WAT1-related protein At3g30340-like isoform X1 [Momordica charantia][more]
XP_004145962.21.3e-13272.65WAT1-related protein At3g30340 [Cucumis sativus] >KGN49962.1 hypothetical protei... [more]
Match NameE-valueIdentityDescription
Q9LI651.0e-7944.41WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2... [more]
Q9M1303.1e-7343.56WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2... [more]
Q5XEZ01.1e-6742.01WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2... [more]
Q9M1292.6e-6442.50WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2... [more]
Q9M1314.2e-6239.29WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1DX699.1e-19399.72WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111024913 PE=3 SV=1[more]
A0A6J1DS663.6e-15785.80WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111023912 PE=3 SV=1[more]
A0A6J1DS722.3e-14076.08WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111023915 PE=3 SV=1[more]
A0A6J1DQS84.0e-14074.57WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111022703 PE=3 SV=1[more]
A0A0A0KNK76.2e-13372.65WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G146850 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G30340.17.1e-8144.41nodulin MtN21 /EamA-like transporter family protein [more]
AT4G01440.12.2e-7443.56nodulin MtN21 /EamA-like transporter family protein [more]
AT1G01070.18.1e-6942.01nodulin MtN21 /EamA-like transporter family protein [more]
AT4G01450.21.9e-6542.50nodulin MtN21 /EamA-like transporter family protein [more]
AT4G01430.13.0e-6339.29nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 15..151
e-value: 2.6E-14
score: 53.6
coord: 179..317
e-value: 1.6E-8
score: 34.8
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 9..335
NoneNo IPR availablePANTHERPTHR31218:SF237WAT1-RELATED PROTEINcoord: 9..335
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 42..153
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 242..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g37850.1Moc06g37850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity