Moc06g37550 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g37550
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionmethionine gamma-lyase
Locationchr6: 28855200 .. 28857738 (+)
RNA-Seq ExpressionMoc06g37550
SyntenyMoc06g37550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAAGTAGAAGCTCGTCCTATTCCTCACCCATGCTGGAACGAGAAGAAGAAGTTAATCAAGTACGAGAAGGCCAAAAGCCCGAAGCCGGCGACGAAGTCAGCGACCGAGGAGGATCCTGCAGCGGCGCTGGCGTTGGCGGAGCACGAGTTCGGGGAGCACGGCGGAGTGAACATGTCGGTTGAGGCCTCGATCACATTCACCGTGATGGAGGCGGAAACGATGCGGCGGATGTTCGAAGGGGAGGAGGGCCCAGAAGGGGAGTGCTTCATCTACAGCCGTCACTTCAACCCGACGGTGATGGCTCTCAGCCGTCAGATGGCGGCGCTGGAAGGAACTGAGGCGGCGTATTGCACTTCCAGTGGGATGGCCGCCGTTTCTTCGGTTTTGCTTCAGCTGGTGAGCTCCGGCGGCCATATTGTGGCCTCGAGGACTCTCTACGGCGGCACTCACGCGCTTCTCTCGCATTTTCTGCCGAGGGTCTGCAACATTACAACCACGTTTGTGGATATTAAGGACCATGGGATGGTTGCAAATGCCATGCTGGAGGGGAAGACCAAGGTGAGTTCATTCCAAAACTTTTAAGTTTTTAGGTTAAAACTTAACTTAAATTTATATGTTATTCATGCCGTTAAATTATAAATTTGGTCTCTATAGTTTAAACATAGTTTCAATGTCTAGATTTAGTCTAGTGTGGACTTAACTCCAACTTGGTCTTTATAGCTTTAGTTTAGTTTTGTTTCAATTAAGTGATTCAGATTTAAAAATTTTAAATTGATTGGTGTGGTTTACTCAAACTTAATAGAACCTAATATCTTGTGACTTTTTTTCTTTTTATTTTAATTTAAGAATTTTTTAGGGTGAGAATCGAACTATCCATCTTTTAGATATTTAGTTGTATTTGAATTTGAATTTTAGTTGTATTTGAATTTGAATTGCACGTGTAGTTACTATATCAATAATTTTTTTACATGATAATAAGATAATGCTTTATATGTGGATAAATATATTTAGTTAGTTATAGTAGGGTTTAAAGATTGAGTAAAAATAATAGTAATAATAAATAAATAAGTATAATGTAAGTGGTTAAATTTAGAGATGATTTATTGTGAGTTCAAAGGAAAAACTAATAAGAGTAACTAAATTGAAATTTTTAAAATTACAAATAATAGCGAATGCAAATTTTCGAACAAATTGATTATTTATATTAAGTTGGGAGAAGATAACAAATGAGTTCTTCCTATAATTTCCTACTAAAATATTACGGTGAAGGATCACCGTAAAAAAAAAACTTTAAGTTTATGGATTATGGTAAATCTAATCTTTTATTTATATTCTCGACAAGGTGTTGTATTTCGAGACAGTGGCGAACCCGACCCTGACCGTGGCGGACATACCGGAGCTAAGTCGGATGGCTCATGAAAAGGGGGTGACGGTGGTCGTGGATAATACGTTCGCACCAATGGTGATCTCACCGGTGAGGCTTGGAGCTGACGTCGTGGTTCACAGCTCCTCCAAGTTCATCAGCGGCGGCGCCGACATTATTGCCGGTATGACGATAGCGCCATTTGTCTCTCTACTTTTTTATAGTTCAGGGAATTGGAGATTACATGTGCCTTATCCACGTTGTTCTGTCAAACTAATATATATTTAATAATGTATTTGAGATTTTAAAACACCAATTTGCTACCCTAATCATACTTAAGCCTTTTTCGAGAAAAAAAAAAAAACCTTTAGCCCATTACTTAATAGCCTCCACCCAAGCAATTATGTAAGTTTTTTTTTTTTTTTTAATTAATTGTGGGGAGGAGAATATTTGGCGACTATACATCTGGAGGAGCTCCGGCTGACTTTCTGATGTGGAATAATTCGTGTCAAAATATTAGTAAAATGTGAACCACTAGTGAAAACTATTTTAAAAGTATGAAGACTAAATTTATAATTTAACATGGATATTTATATGGTTTGCATAGGTGCTGTTTGTGGGTCAAAAAAATTGGTGCATTCGATGATGGACCTGCAGCAGGGGACACTGATGCTTCTAGGGCCAACAATGAACCCCAAAGTTGCTTGTGAACTGTCACAAAGAGTCCCCCATTTAGGGCTCCGAATGAAAGAGCACTGCCACAGAGCTCTAATCTTCGCCCAAAGAATACAGAACCTTGGCTTCAAAGTGATATATCCAGGCCTCAAGGATCACCCTGACCATCACCTCATAAACTCCATAGCCAACACAAGCTATGGCTATGGCTATGGCGGCGTTCTGTGCATCGACATGGAAACCGAGGAGCGAGCCAATAGGCTCATGAATCTCCTTCAAAACCACACCAAGTTTGGGCTCATGGCCGTCAGCTTGGGGTTCTTCGAGACCCTTATGTCGTGCTCCGGCAGCAGCACCAGCAGCGAGCTGAACGCCGAGGAGAAGGAACTGGCTGGCATTTCTCCCGGCTTGGTTAGGATTTCTGTTGGCTATGTGGGAACCTTGGAGCAGAAGTGGAGCCAATTGGAGAGGGGCTTGTTGAAGATGAAGCTTTGA

mRNA sequence

ATGGCTGAAGTAGAAGCTCGTCCTATTCCTCACCCATGCTGGAACGAGAAGAAGAAGTTAATCAAGTACGAGAAGGCCAAAAGCCCGAAGCCGGCGACGAAGTCAGCGACCGAGGAGGATCCTGCAGCGGCGCTGGCGTTGGCGGAGCACGAGTTCGGGGAGCACGGCGGAGTGAACATGTCGGTTGAGGCCTCGATCACATTCACCGTGATGGAGGCGGAAACGATGCGGCGGATGTTCGAAGGGGAGGAGGGCCCAGAAGGGGAGTGCTTCATCTACAGCCGTCACTTCAACCCGACGGTGATGGCTCTCAGCCGTCAGATGGCGGCGCTGGAAGGAACTGAGGCGGCGTATTGCACTTCCAGTGGGATGGCCGCCGTTTCTTCGGTTTTGCTTCAGCTGGTGAGCTCCGGCGGCCATATTGTGGCCTCGAGGACTCTCTACGGCGGCACTCACGCGCTTCTCTCGCATTTTCTGCCGAGGGTCTGCAACATTACAACCACGTTTGTGGATATTAAGGACCATGGGATGGTTGCAAATGCCATGCTGGAGGGGAAGACCAAGGTGTTGTATTTCGAGACAGTGGCGAACCCGACCCTGACCGTGGCGGACATACCGGAGCTAAGTCGGATGGCTCATGAAAAGGGGGTGACGGTGGTCGTGGATAATACGTTCGCACCAATGGTGATCTCACCGGTGAGGCTTGGAGCTGACGTCGTGGTTCACAGCTCCTCCAAGTTCATCAGCGGCGGCGCCGACATTATTGCCGGTGCTGTTTGTGGGTCAAAAAAATTGGTGCATTCGATGATGGACCTGCAGCAGGGGACACTGATGCTTCTAGGGCCAACAATGAACCCCAAAGTTGCTTGTGAACTGTCACAAAGAGTCCCCCATTTAGGGCTCCGAATGAAAGAGCACTGCCACAGAGCTCTAATCTTCGCCCAAAGAATACAGAACCTTGGCTTCAAAGTGATATATCCAGGCCTCAAGGATCACCCTGACCATCACCTCATAAACTCCATAGCCAACACAAGCTATGGCTATGGCTATGGCGGCGTTCTGTGCATCGACATGGAAACCGAGGAGCGAGCCAATAGGCTCATGAATCTCCTTCAAAACCACACCAAGTTTGGGCTCATGGCCGTCAGCTTGGGGTTCTTCGAGACCCTTATGTCGTGCTCCGGCAGCAGCACCAGCAGCGAGCTGAACGCCGAGGAGAAGGAACTGGCTGGCATTTCTCCCGGCTTGGTTAGGATTTCTGTTGGCTATGTGGGAACCTTGGAGCAGAAGTGGAGCCAATTGGAGAGGGGCTTGTTGAAGATGAAGCTTTGA

Coding sequence (CDS)

ATGGCTGAAGTAGAAGCTCGTCCTATTCCTCACCCATGCTGGAACGAGAAGAAGAAGTTAATCAAGTACGAGAAGGCCAAAAGCCCGAAGCCGGCGACGAAGTCAGCGACCGAGGAGGATCCTGCAGCGGCGCTGGCGTTGGCGGAGCACGAGTTCGGGGAGCACGGCGGAGTGAACATGTCGGTTGAGGCCTCGATCACATTCACCGTGATGGAGGCGGAAACGATGCGGCGGATGTTCGAAGGGGAGGAGGGCCCAGAAGGGGAGTGCTTCATCTACAGCCGTCACTTCAACCCGACGGTGATGGCTCTCAGCCGTCAGATGGCGGCGCTGGAAGGAACTGAGGCGGCGTATTGCACTTCCAGTGGGATGGCCGCCGTTTCTTCGGTTTTGCTTCAGCTGGTGAGCTCCGGCGGCCATATTGTGGCCTCGAGGACTCTCTACGGCGGCACTCACGCGCTTCTCTCGCATTTTCTGCCGAGGGTCTGCAACATTACAACCACGTTTGTGGATATTAAGGACCATGGGATGGTTGCAAATGCCATGCTGGAGGGGAAGACCAAGGTGTTGTATTTCGAGACAGTGGCGAACCCGACCCTGACCGTGGCGGACATACCGGAGCTAAGTCGGATGGCTCATGAAAAGGGGGTGACGGTGGTCGTGGATAATACGTTCGCACCAATGGTGATCTCACCGGTGAGGCTTGGAGCTGACGTCGTGGTTCACAGCTCCTCCAAGTTCATCAGCGGCGGCGCCGACATTATTGCCGGTGCTGTTTGTGGGTCAAAAAAATTGGTGCATTCGATGATGGACCTGCAGCAGGGGACACTGATGCTTCTAGGGCCAACAATGAACCCCAAAGTTGCTTGTGAACTGTCACAAAGAGTCCCCCATTTAGGGCTCCGAATGAAAGAGCACTGCCACAGAGCTCTAATCTTCGCCCAAAGAATACAGAACCTTGGCTTCAAAGTGATATATCCAGGCCTCAAGGATCACCCTGACCATCACCTCATAAACTCCATAGCCAACACAAGCTATGGCTATGGCTATGGCGGCGTTCTGTGCATCGACATGGAAACCGAGGAGCGAGCCAATAGGCTCATGAATCTCCTTCAAAACCACACCAAGTTTGGGCTCATGGCCGTCAGCTTGGGGTTCTTCGAGACCCTTATGTCGTGCTCCGGCAGCAGCACCAGCAGCGAGCTGAACGCCGAGGAGAAGGAACTGGCTGGCATTTCTCCCGGCTTGGTTAGGATTTCTGTTGGCTATGTGGGAACCTTGGAGCAGAAGTGGAGCCAATTGGAGAGGGGCTTGTTGAAGATGAAGCTTTGA

Protein sequence

MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWSQLERGLLKMKL
Homology
BLAST of Moc06g37550 vs. NCBI nr
Match: XP_022159757.1 (methionine gamma-lyase [Momordica charantia])

HSP 1 Score: 877.5 bits (2266), Expect = 4.9e-251
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 1   MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM 60
           MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM
Sbjct: 1   MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM 60

Query: 61  SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT 120
           SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT
Sbjct: 61  SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT 120

Query: 121 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN 180
           SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN
Sbjct: 121 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN 180

Query: 181 AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV 240
           AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV
Sbjct: 181 AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV 240

Query: 241 VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG 300
           VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG
Sbjct: 241 VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG 300

Query: 301 LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET 360
           LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET
Sbjct: 301 LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET 360

Query: 361 EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS 420
           EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS
Sbjct: 361 EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS 420

Query: 421 VGYVGTLEQKWSQLERGLLKMKL 444
           VGYVGTLEQKWSQLERGLLKMKL
Sbjct: 421 VGYVGTLEQKWSQLERGLLKMKL 443

BLAST of Moc06g37550 vs. NCBI nr
Match: XP_038875936.1 (methionine gamma-lyase-like [Benincasa hispida])

HSP 1 Score: 710.3 bits (1832), Expect = 1.0e-200
Identity = 362/442 (81.90%), Postives = 393/442 (88.91%), Query Frame = 0

Query: 3   EVEARPIPHPCWNEKKKLIKYE-KAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNMS 62
           EV+A     PCWN   K   Y+ K+ +P   +    +EDPAA LA+A  EFGEHGGVNMS
Sbjct: 4   EVKAHDTSLPCWNIDNKKFSYQTKSATPMVTSWPMGDEDPAATLAMARPEFGEHGGVNMS 63

Query: 63  VEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTS 122
           +E SITFTVMEAETMR MFEGE GPE + FIYSRHFNPTV+ALSRQMAALEGTEAAYCTS
Sbjct: 64  IENSITFTVMEAETMRMMFEGELGPERDFFIYSRHFNPTVLALSRQMAALEGTEAAYCTS 123

Query: 123 SGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANA 182
           SGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVD+KDH MV +A
Sbjct: 124 SGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDA 183

Query: 183 MLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVV 242
           M+EGKTKVLYFETVANPTLTVA++PELSRMAHEKGVTVVVDNTF+PM+ISP RLGADVVV
Sbjct: 184 MVEGKTKVLYFETVANPTLTVANVPELSRMAHEKGVTVVVDNTFSPMIISPARLGADVVV 243

Query: 243 HSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGL 302
           HS SKFISGGADIIAGAVCGSK LV+SMMDL QGTLMLLGPTMN KVA E+SQR+PHLGL
Sbjct: 244 HSCSKFISGGADIIAGAVCGSKNLVNSMMDLHQGTLMLLGPTMNAKVASEISQRIPHLGL 303

Query: 303 RMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETE 362
           RMKEH HRAL+FAQR++NLG KV YPGL DHPDH L++ IAN    YGYGGVLCIDM TE
Sbjct: 304 RMKEHSHRALVFAQRMKNLGLKVTYPGLNDHPDHQLLSYIANKD--YGYGGVLCIDMGTE 363

Query: 363 ERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISV 422
           ERANRLMNLLQNHT FGLMAVSLGFFETLMSCSGSSTSSELN++EKELAGISPGL+R+SV
Sbjct: 364 ERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNSKEKELAGISPGLIRMSV 423

Query: 423 GYVGTLEQKWSQLERGLLKMKL 444
           GYVGTLEQKWSQLE+ LLKMK+
Sbjct: 424 GYVGTLEQKWSQLEKALLKMKI 443

BLAST of Moc06g37550 vs. NCBI nr
Match: KAG7017043.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 703.0 bits (1813), Expect = 1.7e-198
Identity = 356/431 (82.60%), Postives = 388/431 (90.02%), Query Frame = 0

Query: 14  WNEKKKLIKYEKAKSPKPAT-KSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVME 73
           W+ K K +K+     P P T +   EEDPAA+LA A  EFGEHGGVN+S+E SITFTVME
Sbjct: 8   WDVKNKRLKHTAPPPPPPVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVME 67

Query: 74  AETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLL 133
           AETMR+MFEGE GPE + FIYSRHFNPTVMAL RQMAA+EGTEAAYCTSSGMAA+SSVLL
Sbjct: 68  AETMRKMFEGELGPEQDFFIYSRHFNPTVMALGRQMAAIEGTEAAYCTSSGMAAISSVLL 127

Query: 134 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYF 193
           QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVD+KDH  V +AM++ KTKVLYF
Sbjct: 128 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAKVRDAMVKDKTKVLYF 187

Query: 194 ETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGA 253
           ETVANPTLTVA+IPELSRMAHEKGVTVVVDNTF+PM+ISP RLGADVVVHS SKFISGGA
Sbjct: 188 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 247

Query: 254 DIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALI 313
           DIIAGAVCGSKKLV+SMMDL QGTLMLLGPTMN KVA ELSQRVPHL LRMKEH HRAL+
Sbjct: 248 DIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALV 307

Query: 314 FAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQ 373
           FAQR++NLG KVIYPGLKDHPDHHL++SIAN    YGYGGVLCIDM TEERANRLMNLLQ
Sbjct: 308 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKD--YGYGGVLCIDMGTEERANRLMNLLQ 367

Query: 374 NHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWS 433
           NHT FGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGL+R+SVGYVGTLEQKWS
Sbjct: 368 NHTHFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWS 427

Query: 434 QLERGLLKMKL 444
           QLE+ LLKM +
Sbjct: 428 QLEKALLKMNI 436

BLAST of Moc06g37550 vs. NCBI nr
Match: KAG6579584.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 698.4 bits (1801), Expect = 4.1e-197
Identity = 355/431 (82.37%), Postives = 386/431 (89.56%), Query Frame = 0

Query: 14  WNEKKKLIKYEKAKSPKPAT-KSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVME 73
           W+ K K +K+     P P T +   EEDPAA+LA A  EFGEHGGVN+S+E SITFTVME
Sbjct: 8   WDVKNKRLKHTAPPPPPPVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVME 67

Query: 74  AETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLL 133
           AETM +MFEGE GPE + FIYSRHFNPTVMAL RQMAALEGTEAAYCTSSGMAA+SSVLL
Sbjct: 68  AETMSKMFEGELGPEQDFFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAISSVLL 127

Query: 134 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYF 193
           QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVD+KDH  V +AM++ KTKVLYF
Sbjct: 128 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAKVRDAMVKDKTKVLYF 187

Query: 194 ETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGA 253
           ETVANPTLTVA+IPELSRMAHEKGVTVVVDNTF+PM+ISP RLGADVVVHS SKFISG A
Sbjct: 188 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGRA 247

Query: 254 DIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALI 313
           DIIAGAVCGSKKLV+SMMDL QGTLMLLGPTMN KVA ELSQRVPHL LRMKEH HRAL+
Sbjct: 248 DIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALV 307

Query: 314 FAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQ 373
           FAQR++NLG KVIYPGLKDHPDHHL++SIAN    YGYGGVLCIDM TEERANRLMNLLQ
Sbjct: 308 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKD--YGYGGVLCIDMGTEERANRLMNLLQ 367

Query: 374 NHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWS 433
           NHT FGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGL+R+SVGYVGTLEQKWS
Sbjct: 368 NHTHFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWS 427

Query: 434 QLERGLLKMKL 444
           QLE+ LLKM +
Sbjct: 428 QLEKALLKMNI 436

BLAST of Moc06g37550 vs. NCBI nr
Match: XP_022970043.1 (methionine gamma-lyase-like [Cucurbita maxima])

HSP 1 Score: 697.2 bits (1798), Expect = 9.1e-197
Identity = 352/431 (81.67%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 14  WNEKKKLIKYEKAKSPKPAT-KSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVME 73
           W+ K K +K      P P T +   EEDPAA+LA    EFGEHGGVN+S+E SI+FTVME
Sbjct: 3   WDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVME 62

Query: 74  AETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLL 133
           AETMR+MFEGE GPE +CFIYSRHFNPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLL
Sbjct: 63  AETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLL 122

Query: 134 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYF 193
           QL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVD+KDH MV +AM++GKTKVLYF
Sbjct: 123 QLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYF 182

Query: 194 ETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGA 253
           ETVANPTLTVA+IPELSRMAHEKGVTVVVDNTF+PM+ISP RLGADVVVHS SKFISGGA
Sbjct: 183 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 242

Query: 254 DIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALI 313
           DIIAGAVCG KKLV+SMMDL QGTLMLLGPTMN KVA ELSQRVPHL LRMKEH HR L+
Sbjct: 243 DIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLV 302

Query: 314 FAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQ 373
           FAQR++NLG KVIYPGLKDHPDHHL++SIAN    YGYGGVLCIDM TEERANRLMNLLQ
Sbjct: 303 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKD--YGYGGVLCIDMGTEERANRLMNLLQ 362

Query: 374 NHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWS 433
           NHT FG +AVSLG+FETL+SCSGSSTSSE+N EEKELAGISPGL+R+SV YVGTLEQKWS
Sbjct: 363 NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS 422

Query: 434 QLERGLLKMKL 444
           QLE+ LLK+ +
Sbjct: 423 QLEKALLKLNI 431

BLAST of Moc06g37550 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 2.9e-177
Identity = 307/412 (74.51%), Postives = 358/412 (86.89%), Query Frame = 0

Query: 30  KPATKSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGE 89
           K +  +  + DPAAA+A   HEFGEHGGVNMS+EAS TFTVME +TMRRMF GE GP+ +
Sbjct: 32  KKSALAVCDADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDND 91

Query: 90  CFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYG 149
            ++YSRHFNPTV+ LSRQMAALEGT+AAYCTSSGM+A+SSV+LQL SSGGH+VA+ TLYG
Sbjct: 92  FYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYG 151

Query: 150 GTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANPTLTVADIPELS 209
           GTHALLSHFLPR CNITT+FVDI DHG VANA++EG+T+VLYFE+VANPTLTVADIPELS
Sbjct: 152 GTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELS 211

Query: 210 RMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSM 269
           RMAHEKGVTVVVDNTFAPMV+SP +LGADVVVHS SKFISGGADIIAGAVCGS+ LV  M
Sbjct: 212 RMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEM 271

Query: 270 MDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALIFAQRIQNLGFKVIYPGL 329
           MDL+ G+LMLLGPTMN KVA ELS+R+PHLGLRM+EH HRA ++A+R+++LG KVIYPGL
Sbjct: 272 MDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGL 331

Query: 330 KDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFET 389
           + HP H L   + N    YGYGG+L IDMETEE+AN+LM  LQN T+FG MAVSLG++ET
Sbjct: 332 ETHPQHKLFKGMVNRD--YGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYET 391

Query: 390 LMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWSQLERGLLKM 442
           LMSCSGSSTSSEL+  +KE AGISPGLVR+SVGYVGTLEQKW+Q E+  L+M
Sbjct: 392 LMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of Moc06g37550 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.1e-54
Identity = 150/392 (38.27%), Postives = 218/392 (55.61%), Query Frame = 0

Query: 55  HGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGT 114
           +G + M +  + TF    AE   R F  EE      +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALEEAG----YIYTRLGNPTTTVLENKIAALEEG 81

Query: 115 EAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKD 174
           EAA  TSSGM A+SS L  ++ +G H+V  +TLYG T AL+ H L R   I  TFVD  +
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTRF-GIEVTFVDTSN 141

Query: 175 HGMVANAMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVT-VVVDNTFA-PMVISP 234
              V NAM +  T+V+Y ET ANP L + D+  LS++AH    T V+VDNTFA P +  P
Sbjct: 142 LDEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 235 VRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACEL 294
           ++LGAD+VVHS +K+I+G  D+IAG V  +K+L   +  +  G   + G  + P+ A  +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 295 SQRVPHLGLRMKEHCHRA---LIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYG 354
            + +    +RM+ HC  A   + F  +   +  +V YPGL+ HP H +            
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIE-RVYYPGLETHPGHEIAKKQMK-----D 321

Query: 355 YGGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKEL 414
           +G ++  +++    A +    L N+ K   +AVSLG  ETL+    S T S    EE+E 
Sbjct: 322 FGAMISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREA 381

Query: 415 AGISPGLVRISVGYVGTLEQKWSQLERGLLKM 442
           AGI+ GLVR+SVG +  +E   + LE+GL K+
Sbjct: 382 AGITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of Moc06g37550 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.9e-52
Identity = 143/391 (36.57%), Postives = 214/391 (54.73%), Query Frame = 0

Query: 55  HGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGT 114
           +G + M +  + TF    AE   R F  EE      +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALEEAG----YIYTRLGNPTTTVLENKIAALEEG 81

Query: 115 EAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKD 174
           EA    SSGM A+SS L  ++ +G H+V  +TLYG T AL++H L R   +  TFVD  +
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTRF-GVEVTFVDTSN 141

Query: 175 HGMVANAMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVT-VVVDNTFA-PMVISP 234
              V NAM +  T+V+Y ET ANP L + D+  LS++AH    T V+VDNTFA P +  P
Sbjct: 142 LEEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 235 VRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACEL 294
           ++LG D+VVHS++K+++G  D+IAG V   ++L   +  +  G   + G  + P+ A  +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 295 SQRVPHLGLRMKEHCHRALIFAQRIQN--LGFKVIYPGLKDHPDHHLINSIANTSYGYGY 354
            + +    +RM+ HC  A      +       KV YPGL+ HP + +            +
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGYEIAKKQMK-----DF 321

Query: 355 GGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELA 414
           G ++  +++    A +    L N+ K   +AVSLG  ETL+    S T S    EE+E+A
Sbjct: 322 GAMISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREVA 381

Query: 415 GISPGLVRISVGYVGTLEQKWSQLERGLLKM 442
           GI+ GLVR+SVG +  +E   + LE+GL K+
Sbjct: 382 GITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of Moc06g37550 vs. ExPASy Swiss-Prot
Match: Q73KL7 (L-methionine gamma-lyase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) OX=243275 GN=megL PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.3e-48
Identity = 133/374 (35.56%), Postives = 201/374 (53.74%), Query Frame = 0

Query: 54  EHGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEG 113
           ++G +   +  + TF    AE   R F  EE    E +IY+R  NPT   +  ++A LE 
Sbjct: 24  KYGALATPIYQTSTFAFDSAEQGGRRFALEE----EGYIYTRLGNPTTTVVEEKLACLEN 83

Query: 114 TEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIK 173
            EA    SSG+ AV+S +  +V++G HIVA +TLYG T A L+H L R   +  TFVD +
Sbjct: 84  GEACMSASSGIGAVTSCIWSIVNAGDHIVAGKTLYGCTFAFLNHGLSRF-GVDVTFVDTR 143

Query: 174 DHGMVANAMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKG--VTVVVDNTF-APMVI 233
           D   V  A L+  TK++Y ET ANP + + DI  +S++AH       V+VDNT+  P + 
Sbjct: 144 DPENVKKA-LKPNTKIVYLETPANPNMYLCDIAAVSKIAHAHNPECKVIVDNTYMTPYLQ 203

Query: 234 SPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVAC 293
            P+ LGADVV+HS++K+++G  D+IAG V G K+ +  +  +  G   + G T+ P  A 
Sbjct: 204 RPLDLGADVVLHSATKYLNGHGDVIAGFVVGKKEFIDQVRFV--GVKDMTGSTLGPFEAY 263

Query: 294 ELSQRVPHLGLRMKEHCHRALIFAQRIQN--LGFKVIYPGLKDHPDHHLINSIANTSYGY 353
            + + +  L +RM++HC  A   A+ ++       + +PGLK  P + L           
Sbjct: 264 LIGRGMKTLDIRMEKHCANAQKVAEFLEKHPAVESIAFPGLKSFPQYELAKKQMKLC--- 323

Query: 354 GYGGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKE 413
             G ++   ++    A +    L N  KF  +AVSLG  ETL+    S T S    EE+ 
Sbjct: 324 --GAMIAFTVKGGLEAGK---TLINSVKFATIAVSLGDAETLIQHPASMTHSPYTPEERA 381

Query: 414 LAGISPGLVRISVG 423
            + I+ GLVR+SVG
Sbjct: 384 ASDIAEGLVRLSVG 381

BLAST of Moc06g37550 vs. ExPASy Swiss-Prot
Match: P13254 (L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2)

HSP 1 Score: 189.9 bits (481), Expect = 6.2e-47
Identity = 139/374 (37.17%), Postives = 197/374 (52.67%), Query Frame = 0

Query: 54  EHGGVNM-SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALE 113
           +HGG  +  V  + TFT    E     F GE+        YSR  NPT+  L  +MA+LE
Sbjct: 23  DHGGALVPPVYQTATFTFPTVEYGAACFAGEQAGH----FYSRISNPTLNLLEARMASLE 82

Query: 114 GTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDI 173
           G EA    +SGM A++S L  L+  G  ++   TLYG T A L H +     +    VD+
Sbjct: 83  GGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGEF-GVKLRHVDM 142

Query: 174 KDHGMVANAMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTF-APMVIS 233
            D   +  AM    T+V+YFE+ ANP + +ADI  ++++A + G TVVVDNT+  P +  
Sbjct: 143 ADLQALEAAMTPA-TRVIYFESPANPNMHMADIAGVAKIARKHGATVVVDNTYCTPYLQR 202

Query: 234 PVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACE 293
           P+ LGAD+VVHS++K++SG  DI AG V GS+ LV  +    QG   + G  ++P  A  
Sbjct: 203 PLELGADLVVHSATKYLSGHGDITAGIVVGSQALVDRIR--LQGLKDMTGAVLSPHDAAL 262

Query: 294 LSQRVPHLGLRMKEHCHRALIFAQRI--QNLGFKVIYPGLKDHPDHHLINSIANTSYGYG 353
           L + +  L LRM  HC  A + A+ +  Q     + YPGL   P + L     +      
Sbjct: 263 LMRGIKTLNLRMDRHCANAQVLAEFLARQPQVELIHYPGLASFPQYTLARQQMSQP---- 322

Query: 354 YGGVLCIDMETEERA-NRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKE 413
            GG++  +++    A  R MN LQ  ++    AVSLG  E+L     S T S    EE+ 
Sbjct: 323 -GGMIAFELKGGIGAGRRFMNALQLFSR----AVSLGDAESLAQHPASMTHSSYTPEERA 379

Query: 414 LAGISPGLVRISVG 423
             GIS GLVR+SVG
Sbjct: 383 HYGISEGLVRLSVG 379

BLAST of Moc06g37550 vs. ExPASy TrEMBL
Match: A0A6J1E0P6 (methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.4e-251
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 1   MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM 60
           MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM
Sbjct: 1   MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM 60

Query: 61  SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT 120
           SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT
Sbjct: 61  SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT 120

Query: 121 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN 180
           SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN
Sbjct: 121 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN 180

Query: 181 AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV 240
           AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV
Sbjct: 181 AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV 240

Query: 241 VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG 300
           VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG
Sbjct: 241 VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG 300

Query: 301 LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET 360
           LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET
Sbjct: 301 LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET 360

Query: 361 EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS 420
           EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS
Sbjct: 361 EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS 420

Query: 421 VGYVGTLEQKWSQLERGLLKMKL 444
           VGYVGTLEQKWSQLERGLLKMKL
Sbjct: 421 VGYVGTLEQKWSQLERGLLKMKL 443

BLAST of Moc06g37550 vs. ExPASy TrEMBL
Match: A0A6J1I2P5 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.4e-197
Identity = 352/431 (81.67%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 14  WNEKKKLIKYEKAKSPKPAT-KSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVME 73
           W+ K K +K      P P T +   EEDPAA+LA    EFGEHGGVN+S+E SI+FTVME
Sbjct: 3   WDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVME 62

Query: 74  AETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLL 133
           AETMR+MFEGE GPE +CFIYSRHFNPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLL
Sbjct: 63  AETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLL 122

Query: 134 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYF 193
           QL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVD+KDH MV +AM++GKTKVLYF
Sbjct: 123 QLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYF 182

Query: 194 ETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGA 253
           ETVANPTLTVA+IPELSRMAHEKGVTVVVDNTF+PM+ISP RLGADVVVHS SKFISGGA
Sbjct: 183 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 242

Query: 254 DIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALI 313
           DIIAGAVCG KKLV+SMMDL QGTLMLLGPTMN KVA ELSQRVPHL LRMKEH HR L+
Sbjct: 243 DIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLV 302

Query: 314 FAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQ 373
           FAQR++NLG KVIYPGLKDHPDHHL++SIAN    YGYGGVLCIDM TEERANRLMNLLQ
Sbjct: 303 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKD--YGYGGVLCIDMGTEERANRLMNLLQ 362

Query: 374 NHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWS 433
           NHT FG +AVSLG+FETL+SCSGSSTSSE+N EEKELAGISPGL+R+SV YVGTLEQKWS
Sbjct: 363 NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS 422

Query: 434 QLERGLLKMKL 444
           QLE+ LLK+ +
Sbjct: 423 QLEKALLKLNI 431

BLAST of Moc06g37550 vs. ExPASy TrEMBL
Match: A0A5A7TIP4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001500 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.9e-196
Identity = 353/403 (87.59%), Postives = 374/403 (92.80%), Query Frame = 0

Query: 40  DPAAALALAEHEFGEHGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNP 99
           DPAAALA+A  EFGEHGGVNMS+E SITFTVMEAETMRRMFEGE GPE + FIYSRHFNP
Sbjct: 41  DPAAALAMARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNP 100

Query: 100 TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 159
           TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL
Sbjct: 101 TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 160

Query: 160 PRVCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTV 219
           PRV  ITTTFVD+++H MV +AM+EGKTKVLYFETVANPTLTVA+IPELSRMAHEKGVTV
Sbjct: 161 PRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTV 220

Query: 220 VVDNTFAPMVISPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLML 279
           VVDNTF+PM+ISP RLGADVVVHS SKFISGGADIIAGAVCGSK LV+SMMDL QGTLML
Sbjct: 221 VVDNTFSPMIISPARLGADVVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLML 280

Query: 280 LGPTMNPKVACELSQRVPHLGLRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLIN 339
           LGPTMN KVA ELSQR+PHLGLRMKEH +RAL+FAQR++NLG KVIYPGL DHPDH L+ 
Sbjct: 281 LGPTMNAKVASELSQRIPHLGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLG 340

Query: 340 SIANTSYGYGYGGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTS 399
           SIAN    YGYGGVLCIDM TEERANRLMNLLQNHT FGLMAVSLGFFETLMSCSGSSTS
Sbjct: 341 SIANKD--YGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTS 400

Query: 400 SELNAEEKELAGISPGLVRISVGYVGTLEQKWSQLERGLLKMK 443
           SELN EEKELAGISPGL+R+SVGYVGTLEQKWSQLE+ LLKMK
Sbjct: 401 SELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKMK 441

BLAST of Moc06g37550 vs. ExPASy TrEMBL
Match: A0A1S3AU15 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.9e-196
Identity = 353/403 (87.59%), Postives = 374/403 (92.80%), Query Frame = 0

Query: 40  DPAAALALAEHEFGEHGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNP 99
           DPAAALA+A  EFGEHGGVNMS+E SITFTVMEAETMRRMFEGE GPE + FIYSRHFNP
Sbjct: 41  DPAAALAMARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNP 100

Query: 100 TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 159
           TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL
Sbjct: 101 TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 160

Query: 160 PRVCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTV 219
           PRV  ITTTFVD+++H MV +AM+EGKTKVLYFETVANPTLTVA+IPELSRMAHEKGVTV
Sbjct: 161 PRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTV 220

Query: 220 VVDNTFAPMVISPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLML 279
           VVDNTF+PM+ISP RLGADVVVHS SKFISGGADIIAGAVCGSK LV+SMMDL QGTLML
Sbjct: 221 VVDNTFSPMIISPARLGADVVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLML 280

Query: 280 LGPTMNPKVACELSQRVPHLGLRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLIN 339
           LGPTMN KVA ELSQR+PHLGLRMKEH +RAL+FAQR++NLG KVIYPGL DHPDH L+ 
Sbjct: 281 LGPTMNAKVASELSQRIPHLGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLG 340

Query: 340 SIANTSYGYGYGGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTS 399
           SIAN    YGYGGVLCIDM TEERANRLMNLLQNHT FGLMAVSLGFFETLMSCSGSSTS
Sbjct: 341 SIANKD--YGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTS 400

Query: 400 SELNAEEKELAGISPGLVRISVGYVGTLEQKWSQLERGLLKMK 443
           SELN EEKELAGISPGL+R+SVGYVGTLEQKWSQLE+ LLKMK
Sbjct: 401 SELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKMK 441

BLAST of Moc06g37550 vs. ExPASy TrEMBL
Match: A0A0A0KQL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.4e-195
Identity = 357/443 (80.59%), Postives = 387/443 (87.36%), Query Frame = 0

Query: 1   MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM 60
           + EV A     PC N        E     + ++    E DPAAALA+A  EFGEHGGVNM
Sbjct: 2   ITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVNM 61

Query: 61  SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT 120
           S+E SITFTVMEAETMRRMFEGE GPE + FIYSRHFNPTVMALSRQMAALEGTEAAYCT
Sbjct: 62  SIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCT 121

Query: 121 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN 180
           SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVD+++H MV +
Sbjct: 122 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRD 181

Query: 181 AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV 240
           AM+EGKTKVLYFETVANPTLTVA+IPELSRMAH+KGVTVVVDNTF+P++ISP RLGADVV
Sbjct: 182 AMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVV 241

Query: 241 VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG 300
           VHS SKFISGGADIIAGAVCGSK+LV+SMMDL QGTLMLLGPTMN KVA ELSQR+PHL 
Sbjct: 242 VHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLS 301

Query: 301 LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET 360
           LRMKEH +RAL+FAQR++NLG KVIYPGL DHPDH L+ SIAN    YGYGGVLCIDM T
Sbjct: 302 LRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKD--YGYGGVLCIDMGT 361

Query: 361 EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS 420
           EERANRLMNLLQNHT FGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGL+R+S
Sbjct: 362 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 421

Query: 421 VGYVGTLEQKWSQLERGLLKMKL 444
           VGYVGTLEQKW+QLE+ LLKMK+
Sbjct: 422 VGYVGTLEQKWNQLEKALLKMKI 442

BLAST of Moc06g37550 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 622.9 bits (1605), Expect = 2.0e-178
Identity = 307/412 (74.51%), Postives = 358/412 (86.89%), Query Frame = 0

Query: 30  KPATKSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVMEAETMRRMFEGEEGPEGE 89
           K +  +  + DPAAA+A   HEFGEHGGVNMS+EAS TFTVME +TMRRMF GE GP+ +
Sbjct: 32  KKSALAVCDADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDND 91

Query: 90  CFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYG 149
            ++YSRHFNPTV+ LSRQMAALEGT+AAYCTSSGM+A+SSV+LQL SSGGH+VA+ TLYG
Sbjct: 92  FYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYG 151

Query: 150 GTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANPTLTVADIPELS 209
           GTHALLSHFLPR CNITT+FVDI DHG VANA++EG+T+VLYFE+VANPTLTVADIPELS
Sbjct: 152 GTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELS 211

Query: 210 RMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSM 269
           RMAHEKGVTVVVDNTFAPMV+SP +LGADVVVHS SKFISGGADIIAGAVCGS+ LV  M
Sbjct: 212 RMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEM 271

Query: 270 MDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALIFAQRIQNLGFKVIYPGL 329
           MDL+ G+LMLLGPTMN KVA ELS+R+PHLGLRM+EH HRA ++A+R+++LG KVIYPGL
Sbjct: 272 MDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGL 331

Query: 330 KDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQNHTKFGLMAVSLGFFET 389
           + HP H L   + N    YGYGG+L IDMETEE+AN+LM  LQN T+FG MAVSLG++ET
Sbjct: 332 ETHPQHKLFKGMVNRD--YGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYET 391

Query: 390 LMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWSQLERGLLKM 442
           LMSCSGSSTSSEL+  +KE AGISPGLVR+SVGYVGTLEQKW+Q E+  L+M
Sbjct: 392 LMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of Moc06g37550 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-42
Identity = 115/336 (34.23%), Postives = 181/336 (53.87%), Query Frame = 0

Query: 91  FIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGG 150
           F Y R+ NPT + L  +++ALEG E+    +SGM A + +LL LV +GGHIV +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 151 THALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANPTLTVADIPELSR 210
           T   + +FLP++  IT T +D  D   +  A+ E K  + + E+  NP L   DI  +S+
Sbjct: 284 TRIFMENFLPKL-GITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 211 MAHEKGVTVVVDNTFA-PMVISPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVHSM 270
           + H++G  V +D TFA P+    + LGAD+VVHS++K+I G  D++AG +CGS KLV  +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 271 MDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALIFAQRIQ--NLGFKVIYP 330
            +L      +LG T+NP  A  + + +  L LR+++    A   A+ ++       V YP
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVSHVYYP 463

Query: 331 GLKDHPDHHLINSIANTSYGYGYGGVLCIDMETE-ERANRLMNLLQNHTKFGLMAVSLGF 390
           GL  HP+H L           G+GGV+  +++ + E   + ++ L    K   +A S G 
Sbjct: 464 GLPSHPEHELAKRQMT-----GFGGVVSFEIDGDIETTIKFVDSL----KIPYIAPSFGG 523

Query: 391 FETLMSCSGSSTSSELNAEEKELAGISPGLVRISVG 423
            E+++      +  +L  EE+   GI   LVR S G
Sbjct: 524 CESIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFG 545

BLAST of Moc06g37550 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 152.1 bits (383), Expect = 1.0e-36
Identity = 118/349 (33.81%), Postives = 184/349 (52.72%), Query Frame = 0

Query: 83  EEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIV 142
           E GP    + Y+R  NPT  AL   +A L+  + A+C +SGMAA+S+V   L+ +G  IV
Sbjct: 121 ENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAALSAV-THLIKNGEEIV 180

Query: 143 ASRTLYGGTHALLSHFLPR----VCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANP 202
           A   +YGG+  LLS  +PR    V  + TT +D         A +  +TK+++ E+  NP
Sbjct: 181 AGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLD------EVAAAIGPQTKLVWLESPTNP 240

Query: 203 TLTVADIPELSRMAHEKGVTVVVDNT-FAPMVISPVRLGADVVVHSSSKFISGGADIIAG 262
              ++DI ++S MAH +G  V+VDN+  +P++  P+ LGAD+V+HS++KFI+G +D++AG
Sbjct: 241 RQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAG 300

Query: 263 AVC-GSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQR-VPHLGLRMKEHCHRALIFAQ 322
            +    +KL   +  LQ      L P       C L  R +  + LR+++    A   A 
Sbjct: 301 VLAVKGEKLAKEVYFLQNSEGSGLAP-----FDCWLCLRGIKTMALRIEKQQENARKIAM 360

Query: 323 RIQN--LGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQN 382
            + +     KV Y GL DHP HHL     + S   G G V      +   +  L+     
Sbjct: 361 YLSSHPRVKKVYYAGLPDHPGHHL-----HFSQAKGAGSVFSFITGSVALSKHLVET--- 420

Query: 383 HTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVG 423
            TK+  +AVS G  ++L+S     + + + AE +E  G++  LVRIS G
Sbjct: 421 -TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAG 444

BLAST of Moc06g37550 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 152.1 bits (383), Expect = 1.0e-36
Identity = 118/349 (33.81%), Postives = 184/349 (52.72%), Query Frame = 0

Query: 83  EEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIV 142
           E GP    + Y+R  NPT  AL   +A L+  + A+C +SGMAA+S+V   L+ +G  IV
Sbjct: 106 ENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAALSAV-THLIKNGEEIV 165

Query: 143 ASRTLYGGTHALLSHFLPR----VCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANP 202
           A   +YGG+  LLS  +PR    V  + TT +D         A +  +TK+++ E+  NP
Sbjct: 166 AGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLD------EVAAAIGPQTKLVWLESPTNP 225

Query: 203 TLTVADIPELSRMAHEKGVTVVVDNT-FAPMVISPVRLGADVVVHSSSKFISGGADIIAG 262
              ++DI ++S MAH +G  V+VDN+  +P++  P+ LGAD+V+HS++KFI+G +D++AG
Sbjct: 226 RQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAG 285

Query: 263 AVC-GSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQR-VPHLGLRMKEHCHRALIFAQ 322
            +    +KL   +  LQ      L P       C L  R +  + LR+++    A   A 
Sbjct: 286 VLAVKGEKLAKEVYFLQNSEGSGLAP-----FDCWLCLRGIKTMALRIEKQQENARKIAM 345

Query: 323 RIQN--LGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQN 382
            + +     KV Y GL DHP HHL     + S   G G V      +   +  L+     
Sbjct: 346 YLSSHPRVKKVYYAGLPDHPGHHL-----HFSQAKGAGSVFSFITGSVALSKHLVET--- 405

Query: 383 HTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVG 423
            TK+  +AVS G  ++L+S     + + + AE +E  G++  LVRIS G
Sbjct: 406 -TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAG 429

BLAST of Moc06g37550 vs. TAIR 10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-35
Identity = 107/338 (31.66%), Postives = 179/338 (52.96%), Query Frame = 0

Query: 89  ECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLY 148
           + + Y+R+ NPT MAL  +++ LEG E+    +SGM A + +LL LV + GHIVA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 149 GGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYFETVANPTLTVADIPEL 208
             T   + +FL ++  IT TF+D  D   +   +   +  + + E+  NP L   DI  +
Sbjct: 132 KETRIFMENFLTKL-GITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLV 191

Query: 209 SRMAHEKGVTVVVDNTFA-PMVISPVRLGADVVVHSSSKFISGGADIIAGAVCGSKKLVH 268
           S++ H +G  V +D T A P+    + LGAD+V HS++K+I G  D +AG++ GS +LV 
Sbjct: 192 SKICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVS 251

Query: 269 SMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALIFAQRIQ--NLGFKVI 328
            + +L +    LLG T+NP  A  L + +  + LR+++     +  AQ ++      +V 
Sbjct: 252 KIRNLHK----LLGGTLNPNAAYLLIRGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVY 311

Query: 329 YPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERAN-RLMNLLQNHTKFGLMAVSL 388
           Y GL  HP+ HLI     T    G GG++  +++ + +   + ++ L    K   +A S 
Sbjct: 312 YLGLPSHPE-HLIAKRQMT----GIGGLISFEIDGDLKTTIKFIDAL----KIPYLAASF 371

Query: 389 GFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVG 423
           G  E+L+    +    ++  EE+   G    LVR S G
Sbjct: 372 GGCESLVDQLATGI-WDIPREERLKDGFQDNLVRFSFG 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159757.14.9e-251100.00methionine gamma-lyase [Momordica charantia][more]
XP_038875936.11.0e-20081.90methionine gamma-lyase-like [Benincasa hispida][more]
KAG7017043.11.7e-19882.60Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6579584.14.1e-19782.37Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970043.19.1e-19781.67methionine gamma-lyase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SGU92.9e-17774.51Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1[more]
Q8L0X41.1e-5438.27L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ... [more]
Q8RDT42.9e-5236.57L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC... [more]
Q73KL73.3e-4835.56L-methionine gamma-lyase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 /... [more]
P132546.2e-4737.17L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1E0P62.4e-251100.00methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1[more]
A0A6J1I2P54.4e-19781.67methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=... [more]
A0A5A7TIP44.9e-19687.59Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AU154.9e-19687.59methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1[more]
A0A0A0KQL72.4e-19580.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G64660.12.0e-17874.51methionine gamma-lyase [more]
AT3G01120.11.6e-4234.23Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT3G57050.11.0e-3633.81cystathionine beta-lyase [more]
AT3G57050.21.0e-3633.81cystathionine beta-lyase [more]
AT1G33320.11.5e-3531.66Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePIRSFPIRSF001434CGScoord: 27..442
e-value: 8.7E-103
score: 342.0
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePFAMPF01053Cys_Met_Meta_PPcoord: 54..422
e-value: 4.9E-85
score: 285.4
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePANTHERPTHR11808TRANS-SULFURATION ENZYME FAMILY MEMBERcoord: 34..441
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 42..296
e-value: 7.7E-69
score: 233.8
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 300..442
e-value: 2.5E-24
score: 88.0
NoneNo IPR availablePIRSRPIRSR001434-2PIRSR001434-2coord: 94..402
e-value: 2.6E-79
score: 264.4
NoneNo IPR availablePANTHERPTHR11808:SF76METHIONINE GAMMA-LYASE-LIKEcoord: 34..441
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 70..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g37550.1Moc06g37550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019343 cysteine biosynthetic process via cystathionine
biological_process GO:0019346 transsulfuration
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004123 cystathionine gamma-lyase activity
molecular_function GO:0018826 methionine gamma-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity