Moc06g36630 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g36630
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionGEM-like protein 2
Locationchr6: 28133737 .. 28135909 (+)
RNA-Seq ExpressionMoc06g36630
SyntenyMoc06g36630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCATCCCGGTATATGGAAATCATAATCCCTATATCCAATACTGCGCTTCTGCACCTGCCCAACCCGGGAAAAGTTTGCCTTCTTTAACCCTACTCTCCACCATAGTCTTTGTAGTGATTTAGGGTTCTAATTTGTTTTTAAATTTTTGGATCATGTTCTGTAGAAGATAAGATCCGGGGAGCGTTGGGTCGCTGCGGGAAGATGTTGGAAGAGTATGGTAAAATTGCAGAGGAAGCTGCGGACGACGTCTTGCATCACAGTAAGTTTTTCCCTCGATCAATTTTTCTTTTCGATTTAATCATTTGCTTTCTGCAAGAAGATTATTTCTTCTTTCATGCCTAATATATCGTGCAGTAGTTTTCTTTGTGTTCAAATTTTCATAACTAGCCCTTTTTATTGCATTTGATTTGGTAAAGCTTTGAATAAATCGATTCTAATATTGAAATAGTTCATACTGGATTGGTTTTTTTAAGTGAACTTGGCGATACCGAACATGTTAAATTGGCCATGTTTATAATAACGGATAATATCTCTAGATAGTGGGGGCATAGCTTAGAACTGAACTGACAAACATGTAAAATGAGATTTGTTTTTTTTTTTTAGGGCTGCTAATGTTCTATCTCGTTTCCTGCCCTTGTCTAGAATAATGAAGCCTAGAATGTTGCCTCTTTCTTGCTTGATTTGATAACTCTTAATATGCCGTACCTGTTTTGATATCAGTAAAAGTCAGCCCGAGCATTGGCGATGCAGCAAAGGCTAGGCTTATTCAAGGGACGAAATTAGTCACCGAAGGAGGCCCAGAAAAATTATTCCAGCATACATTTGGTGTTATTCCTGGAGACAAATATTTGCATTCATATGCTTGCTATTTGTCAACTTCTTCTGGGCCTGTGAATGGGACTCTTTACATATCCACCAAAAGAGTGGCTTTTGGTAGTGAATCACCTCTCTGTTACTCTTCATCTCCTGGTCAGCCAGAGTGGATGTATTACAAGGTATGTTCAACCCGTTACTATTTCTAATATATCCGAAATCAAAATATTTGTTAATCTTATATTAGTATTTAGTTATTGTAGCCTCTCACATTTTTTACCATTGACTTTAGAAAGCCATTCCTTCTGGCTTAGTTATTGTAGCCTGTATTATAAGCATGTGAATGTGTGTTCTAGGACATTGTGAATGGTAATATGAGTGTGACAACAACTTGACATTAATTGTTTATTTTCATTTTGACAAGAGCACAATTGGCTATATAGGATAGGAACCCCGAATCCTTGAGAAAAAACATTGACCTCAGCTGTTACTGTTAAGATAAATTTCACGTTTTTCCCATGAGATTCATTTTGACTCTCTCTCTACAGTGGACTAACAGGTTAAATTTGTTTATCCATATTAGTGGACTGAATCAGTTCTCCTTTACTAAATACTTCTTTCATTGGAGATTCAAAATTTTTTTGTAAGGATGGTGAAAATTTGACTTCTATTTATAATCTTTCAATTATAAAAAATTAGGGGCAAATTAGTAGGAAAGTTTCAAAACCTTATTCTTTTTTATATGATTTCTTGAGTTTAAGATAAACTAAAGGAGGTAATTGCCCTCTCTCCTTCTTTGCTCCGACCTTGAGGGGTAGTAGGGAAAGGAAAACTAGGCCATGTGGGGAGTTATAATGGTAAGATTTTTAAGTACTTGTTGATCATGGAAACATTTTAGGAAATAAGCAGGAGGAACAAATTTTATCACAACAAAAGGCCTCCCGAGTCTTGCTTAGCTACTCCAGGAACAACACTGTTCCAGATAATATTGCACATGTTAGAATTTGATTTATCCTACATTTCTTCTCAGGCCTCTGAACACTGAACTTTAACTAATGGAATGCTGGCAAAATGAATCATTAGCTTAGCTATGTGCCTTTTTAGTATTTTACTTTGACTGTGGAACTTCTGCTGTTTTCAAGTTGGGCCTTCTCTTATATGTATATCAATTTGTAGGTGGTGATAGAGCTCAACCAGGTGGCAATGATCAACCCGTCTCCAAACCTTCTCAACCCATCAGAAAAAGACATCCACATAGTCACGAGGGACGGTCACGAATTCTGGTTCATGGGATTTATATGTTTCAGCCGGGCCTTAAAGAGTTTAAACGAGGCGTTAAAGCATGCCCATGCCTAG

mRNA sequence

ATGAGCATCCCGGTATATGGAAATCATAATCCCTATATCCAATACTGCGCTTCTGCACCTGCCCAACCCGGGAAAAAAGATAAGATCCGGGGAGCGTTGGGTCGCTGCGGGAAGATGTTGGAAGAGTATGGTAAAATTGCAGAGGAAGCTGCGGACGACGTCTTGCATCACATAAAAGTCAGCCCGAGCATTGGCGATGCAGCAAAGGCTAGGCTTATTCAAGGGACGAAATTAGTCACCGAAGGAGGCCCAGAAAAATTATTCCAGCATACATTTGGTGTTATTCCTGGAGACAAATATTTGCATTCATATGCTTGCTATTTGTCAACTTCTTCTGGGCCTGTGAATGGGACTCTTTACATATCCACCAAAAGAGTGGCTTTTGGTAGTGAATCACCTCTCTGTTACTCTTCATCTCCTGGTCAGCCAGAGTGGATGTATTACAAGGTGGTGATAGAGCTCAACCAGGTGGCAATGATCAACCCGTCTCCAAACCTTCTCAACCCATCAGAAAAAGACATCCACATAGTCACGAGGGACGGTCACGAATTCTGGTTCATGGGATTTATATGTTTCAGCCGGGCCTTAAAGAGTTTAAACGAGGCGTTAAAGCATGCCCATGCCTAG

Coding sequence (CDS)

ATGAGCATCCCGGTATATGGAAATCATAATCCCTATATCCAATACTGCGCTTCTGCACCTGCCCAACCCGGGAAAAAAGATAAGATCCGGGGAGCGTTGGGTCGCTGCGGGAAGATGTTGGAAGAGTATGGTAAAATTGCAGAGGAAGCTGCGGACGACGTCTTGCATCACATAAAAGTCAGCCCGAGCATTGGCGATGCAGCAAAGGCTAGGCTTATTCAAGGGACGAAATTAGTCACCGAAGGAGGCCCAGAAAAATTATTCCAGCATACATTTGGTGTTATTCCTGGAGACAAATATTTGCATTCATATGCTTGCTATTTGTCAACTTCTTCTGGGCCTGTGAATGGGACTCTTTACATATCCACCAAAAGAGTGGCTTTTGGTAGTGAATCACCTCTCTGTTACTCTTCATCTCCTGGTCAGCCAGAGTGGATGTATTACAAGGTGGTGATAGAGCTCAACCAGGTGGCAATGATCAACCCGTCTCCAAACCTTCTCAACCCATCAGAAAAAGACATCCACATAGTCACGAGGGACGGTCACGAATTCTGGTTCATGGGATTTATATGTTTCAGCCGGGCCTTAAAGAGTTTAAACGAGGCGTTAAAGCATGCCCATGCCTAG

Protein sequence

MSIPVYGNHNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFMGFICFSRALKSLNEALKHAHA
Homology
BLAST of Moc06g36630 vs. NCBI nr
Match: XP_022156241.1 (GEM-like protein 1 [Momordica charantia])

HSP 1 Score: 437.2 bits (1123), Expect = 8.0e-119
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0

Query: 1   MSIPVYGNHNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKV 60
           MSIPVYGNHNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKV
Sbjct: 1   MSIPVYGNHNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKV 60

Query: 61  SPSIGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLY 120
           SPSIGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLY
Sbjct: 61  SPSIGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLY 120

Query: 121 ISTKRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRD 180
           ISTKRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRD
Sbjct: 121 ISTKRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRD 180

Query: 181 GHEFWFMGFICFSRALKSLNEALKHAHA 209
           GHEFWFMGFICFSRALKSLNEALKHAHA
Sbjct: 181 GHEFWFMGFICFSRALKSLNEALKHAHA 208

BLAST of Moc06g36630 vs. NCBI nr
Match: XP_023553639.1 (GEM-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 340.9 bits (873), Expect = 7.8e-90
Identity = 160/205 (78.05%), Postives = 183/205 (89.27%), Query Frame = 0

Query: 6   YGNHNPYIQY--CASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPS 65
           +GNHNPY+Q+   AS+ AQPGK+DK+   LGRCGKMLE YGK+AEEAA++V HHIKVSPS
Sbjct: 7   HGNHNPYVQHTPTASSSAQPGKRDKMWDVLGRCGKMLEGYGKMAEEAAENVWHHIKVSPS 66

Query: 66  IGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYIST 125
           I DAAKAR +QGTK++ EGGP+KLFQHTFGVIPG+KYL SYACYL+  SGPVNGTLYIST
Sbjct: 67  ITDAAKARFVQGTKVIAEGGPQKLFQHTFGVIPGEKYLSSYACYLADPSGPVNGTLYIST 126

Query: 126 KRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHE 185
           KRVAF SESPLCYSSSPGQP+W+YYKVVIELNQ+A + P PNLLN SEKDIHIVT+DGHE
Sbjct: 127 KRVAFCSESPLCYSSSPGQPQWVYYKVVIELNQLAALRPCPNLLNTSEKDIHIVTKDGHE 186

Query: 186 FWFMGFICFSRALKSLNEALKHAHA 209
           FWF+GF+ FSRALKSLNEALKH+ A
Sbjct: 187 FWFLGFLLFSRALKSLNEALKHSCA 211

BLAST of Moc06g36630 vs. NCBI nr
Match: XP_022967332.1 (GEM-like protein 2 [Cucurbita maxima])

HSP 1 Score: 340.1 bits (871), Expect = 1.3e-89
Identity = 159/205 (77.56%), Postives = 183/205 (89.27%), Query Frame = 0

Query: 6   YGNHNPYIQY--CASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPS 65
           +GNHNPY+Q+   AS+ AQPGK+DK+   LGRCGKMLE YGK+AEEAA++VLHHIKVSPS
Sbjct: 7   HGNHNPYVQHTSTASSSAQPGKRDKMWDVLGRCGKMLEGYGKMAEEAAENVLHHIKVSPS 66

Query: 66  IGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYIST 125
           I DAAKAR +QGTK++ EGG ++LFQHTFGVIPG+KYLHSYACYL+  SGPVNG LYIST
Sbjct: 67  ITDAAKARFVQGTKVIAEGGSQRLFQHTFGVIPGEKYLHSYACYLADPSGPVNGMLYIST 126

Query: 126 KRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHE 185
           KRVAF SESPLCYSSSPGQP+W+YYKVVIELNQ+A + P PNLLN SEKDIHIVT+DGHE
Sbjct: 127 KRVAFCSESPLCYSSSPGQPQWVYYKVVIELNQLAALRPCPNLLNTSEKDIHIVTKDGHE 186

Query: 186 FWFMGFICFSRALKSLNEALKHAHA 209
           FWF+GF+ FSRALKSLNEALKH+ A
Sbjct: 187 FWFLGFLLFSRALKSLNEALKHSCA 211

BLAST of Moc06g36630 vs. NCBI nr
Match: XP_022963576.1 (GEM-like protein 1 [Cucurbita moschata])

HSP 1 Score: 337.0 bits (863), Expect = 1.1e-88
Identity = 158/205 (77.07%), Postives = 181/205 (88.29%), Query Frame = 0

Query: 6   YGNHNPYIQYCA--SAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPS 65
           +GNHNPY+Q+    S+ AQPGK+DK+   LGRCGKMLE YGK+AEE A++V HHIKVSPS
Sbjct: 7   HGNHNPYVQHTPTDSSSAQPGKRDKMWEVLGRCGKMLEGYGKMAEEVAENVWHHIKVSPS 66

Query: 66  IGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYIST 125
           I DAAKAR +QGTK++ EGGP+KLFQHTFGVIPG+KYL SYACYL+  SGPVNGTLYIST
Sbjct: 67  ITDAAKARFVQGTKVIAEGGPQKLFQHTFGVIPGEKYLSSYACYLADPSGPVNGTLYIST 126

Query: 126 KRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHE 185
           KRVAF SESPLCYSSSPGQP+W+YYKVVIELNQ+A + P PNLLN SEKDIHIVT+DGHE
Sbjct: 127 KRVAFCSESPLCYSSSPGQPQWVYYKVVIELNQLAALRPCPNLLNTSEKDIHIVTKDGHE 186

Query: 186 FWFMGFICFSRALKSLNEALKHAHA 209
           FWF+GF+ FSRALKSLNEALKH+ A
Sbjct: 187 FWFLGFLLFSRALKSLNEALKHSCA 211

BLAST of Moc06g36630 vs. NCBI nr
Match: KAG6571599.1 (GEM-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 336.7 bits (862), Expect = 1.5e-88
Identity = 157/205 (76.59%), Postives = 181/205 (88.29%), Query Frame = 0

Query: 6   YGNHNPYIQYCA--SAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPS 65
           +GNHNPY+Q+    S+ AQPGK+DK+   LGRCGKMLE YGK+AEE A++V HHIKVSPS
Sbjct: 7   HGNHNPYVQHTPTDSSSAQPGKRDKMWDVLGRCGKMLEGYGKMAEEVAENVWHHIKVSPS 66

Query: 66  IGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYIST 125
           I DAAKAR +QGTK++ EGGP+KLFQHTFGVIPG+KY+ SYACYL+  SGPVNGTLYIST
Sbjct: 67  ITDAAKARFVQGTKVIAEGGPQKLFQHTFGVIPGEKYISSYACYLADPSGPVNGTLYIST 126

Query: 126 KRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHE 185
           KRVAF SESPLCYSSSPGQP+W+YYKVVIELNQ+A + P PNLLN SEKDIHIVT+DGHE
Sbjct: 127 KRVAFCSESPLCYSSSPGQPQWVYYKVVIELNQLAALRPCPNLLNTSEKDIHIVTKDGHE 186

Query: 186 FWFMGFICFSRALKSLNEALKHAHA 209
           FWF+GF+ FSRALKSLNEALKH+ A
Sbjct: 187 FWFLGFLLFSRALKSLNEALKHSCA 211

BLAST of Moc06g36630 vs. ExPASy Swiss-Prot
Match: Q9SE96 (GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 3.4e-48
Identity = 90/195 (46.15%), Postives = 131/195 (67.18%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAAK 69
           NPY+   + +PA     D ++  LG+ GKM  +  K AE+ A +   H+K  PS+ DAA 
Sbjct: 65  NPYV---SPSPAPRNTMDSVKDTLGKWGKMAADATKKAEDLAGNFWQHLKTGPSVADAAV 124

Query: 70  ARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAFG 129
           +R+ QGTK++ EGG EK+F+ TF  +P +K L +YACYLSTS+GPV G +Y+ST ++AF 
Sbjct: 125 SRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKTYACYLSTSAGPVLGVMYLSTHKLAFS 184

Query: 130 SESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFMGF 189
           S++PL Y     Q  W YYKVV+  NQ+  +NPS + +N S+K I +++ D HEFWFMGF
Sbjct: 185 SDNPLSYKEGE-QTLWSYYKVVLPANQLKAVNPSTSRVNTSDKYIQVISIDNHEFWFMGF 244

Query: 190 ICFSRALKSLNEALK 205
           + +  A+KSL EA++
Sbjct: 245 VTYESAVKSLQEAVQ 255

BLAST of Moc06g36630 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 3.8e-47
Identity = 94/196 (47.96%), Postives = 130/196 (66.33%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGK-KDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAA 69
           NPY+   +   A   + KDK+   L RCGK +E+  + AE     +  H+K SPSI DAA
Sbjct: 29  NPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAA 88

Query: 70  KARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAF 129
            ARL QGTK++ EGGPE++FQ  FGV+  +K L S+ CY+ST+SGPV G +YIS +R+AF
Sbjct: 89  MARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAF 148

Query: 130 GSESPL-CYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFM 189
            S+  +   SS+ G     YYKVV+E  +++ I+ S N+L PSE+ +H+VTRDG EFWFM
Sbjct: 149 CSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFM 208

Query: 190 GFICFSRALKSLNEAL 204
           GF+ +  A   LN+AL
Sbjct: 209 GFVSYIDAFNCLNKAL 224

BLAST of Moc06g36630 vs. ExPASy Swiss-Prot
Match: Q8S8F8 (GLABRA2 expression modulator OS=Arabidopsis thaliana OX=3702 GN=GEM PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 7.2e-46
Identity = 89/198 (44.95%), Postives = 130/198 (65.66%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGKKD---KIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGD 69
           NPYI    +  +    KD    ++G LGR GK + E  K  E  A +   H++ +PS  D
Sbjct: 96  NPYIARSPAETSDASLKDTMETVKGVLGRWGKRVAEAAKKTESLAGNTWQHLRTAPSFAD 155

Query: 70  AAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRV 129
           AA  R+ Q TK+  EGG EK+F+ TF   P ++ L+S+ACYLSTS+GPV G LYIS+ ++
Sbjct: 156 AAMGRIAQSTKVFAEGGYEKIFRQTFETDPEEQLLNSFACYLSTSAGPVMGVLYISSAKL 215

Query: 130 AFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWF 189
           A+ S++PL Y +   Q EW YYKVVI L+Q+  +NPS +++NP+EK I +++ D HEFWF
Sbjct: 216 AYCSDNPLSYKNG-DQTEWSYYKVVIPLHQLKAVNPSASIVNPAEKYIQVISVDNHEFWF 275

Query: 190 MGFICFSRALKSLNEALK 205
           MGF+ +  A+ SL ++L+
Sbjct: 276 MGFLNYDGAVTSLQDSLQ 292

BLAST of Moc06g36630 vs. ExPASy Swiss-Prot
Match: Q9LYV6 (GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.2e-34
Identity = 73/191 (38.22%), Postives = 110/191 (57.59%), Query Frame = 0

Query: 9   HNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAA 68
           + PY+ Y  S    P   + +   +G    M   + + AE  A ++ H++K  PS+ + A
Sbjct: 72  YQPYVIY--SPVEHPTTNNPLEPVIG----MFHTWSRKAETVARNLWHNLKTGPSMSETA 131

Query: 69  KARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAF 128
             ++    K +T+GG E LF+  FG  P +    ++ACYLST++GPV GT+Y+S  RVAF
Sbjct: 132 WGKVNLTAKAITKGGFESLFRQIFGTEPNETLKKTFACYLSTTTGPVAGTVYLSNARVAF 191

Query: 129 GSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFMG 188
            S+ PL +++  GQ  W YY+VV+ L  VA +NP      P EK I + T DGH+FWFMG
Sbjct: 192 CSDRPLYFTAPSGQESWSYYRVVVPLANVATVNPVVVKETPPEKYIQLTTVDGHDFWFMG 251

Query: 189 FICFSRALKSL 200
           F+ + +A   L
Sbjct: 252 FVNYEKATHHL 256

BLAST of Moc06g36630 vs. ExPASy Swiss-Prot
Match: Q9M063 (Putative GEM-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g40100 PE=3 SV=2)

HSP 1 Score: 137.5 bits (345), Expect = 1.7e-31
Identity = 74/198 (37.37%), Postives = 114/198 (57.58%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGKK---DKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGD 69
           NPY+    +  +    K   + ++G LGR G+ + E    AE  A +   H         
Sbjct: 55  NPYVARAPTETSDASLKETMESVKGVLGRWGRRVGEAAMKAESLAGNTWQHPL------R 114

Query: 70  AAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRV 129
           AA  R+ Q TK++ EGG EK+F+ TF  +P ++  +S+ACYLSTS+GPV G LY+ST ++
Sbjct: 115 AAMGRIAQSTKVLAEGGYEKIFRQTFETVPEEQLQNSFACYLSTSAGPVMGVLYVSTAKL 174

Query: 130 AFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWF 189
           A+ S++ L               VVI L+Q+  +NPS + +NP+EK I +++ D HEFWF
Sbjct: 175 AYCSDTSL---------------VVIPLHQLKSVNPSISTVNPAEKYIQVISVDDHEFWF 231

Query: 190 MGFICFSRALKSLNEALK 205
           MGF+ +  A+ SL + L+
Sbjct: 235 MGFLNYEGAVTSLQDTLQ 231

BLAST of Moc06g36630 vs. ExPASy TrEMBL
Match: A0A6J1DUB7 (GEM-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111023171 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 3.9e-119
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0

Query: 1   MSIPVYGNHNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKV 60
           MSIPVYGNHNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKV
Sbjct: 1   MSIPVYGNHNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKV 60

Query: 61  SPSIGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLY 120
           SPSIGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLY
Sbjct: 61  SPSIGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLY 120

Query: 121 ISTKRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRD 180
           ISTKRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRD
Sbjct: 121 ISTKRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRD 180

Query: 181 GHEFWFMGFICFSRALKSLNEALKHAHA 209
           GHEFWFMGFICFSRALKSLNEALKHAHA
Sbjct: 181 GHEFWFMGFICFSRALKSLNEALKHAHA 208

BLAST of Moc06g36630 vs. ExPASy TrEMBL
Match: A0A6J1HQI2 (GEM-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111466886 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.4e-90
Identity = 159/205 (77.56%), Postives = 183/205 (89.27%), Query Frame = 0

Query: 6   YGNHNPYIQY--CASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPS 65
           +GNHNPY+Q+   AS+ AQPGK+DK+   LGRCGKMLE YGK+AEEAA++VLHHIKVSPS
Sbjct: 7   HGNHNPYVQHTSTASSSAQPGKRDKMWDVLGRCGKMLEGYGKMAEEAAENVLHHIKVSPS 66

Query: 66  IGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYIST 125
           I DAAKAR +QGTK++ EGG ++LFQHTFGVIPG+KYLHSYACYL+  SGPVNG LYIST
Sbjct: 67  ITDAAKARFVQGTKVIAEGGSQRLFQHTFGVIPGEKYLHSYACYLADPSGPVNGMLYIST 126

Query: 126 KRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHE 185
           KRVAF SESPLCYSSSPGQP+W+YYKVVIELNQ+A + P PNLLN SEKDIHIVT+DGHE
Sbjct: 127 KRVAFCSESPLCYSSSPGQPQWVYYKVVIELNQLAALRPCPNLLNTSEKDIHIVTKDGHE 186

Query: 186 FWFMGFICFSRALKSLNEALKHAHA 209
           FWF+GF+ FSRALKSLNEALKH+ A
Sbjct: 187 FWFLGFLLFSRALKSLNEALKHSCA 211

BLAST of Moc06g36630 vs. ExPASy TrEMBL
Match: A0A6J1HGG7 (GEM-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111463872 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 5.5e-89
Identity = 158/205 (77.07%), Postives = 181/205 (88.29%), Query Frame = 0

Query: 6   YGNHNPYIQYCA--SAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPS 65
           +GNHNPY+Q+    S+ AQPGK+DK+   LGRCGKMLE YGK+AEE A++V HHIKVSPS
Sbjct: 7   HGNHNPYVQHTPTDSSSAQPGKRDKMWEVLGRCGKMLEGYGKMAEEVAENVWHHIKVSPS 66

Query: 66  IGDAAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYIST 125
           I DAAKAR +QGTK++ EGGP+KLFQHTFGVIPG+KYL SYACYL+  SGPVNGTLYIST
Sbjct: 67  ITDAAKARFVQGTKVIAEGGPQKLFQHTFGVIPGEKYLSSYACYLADPSGPVNGTLYIST 126

Query: 126 KRVAFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHE 185
           KRVAF SESPLCYSSSPGQP+W+YYKVVIELNQ+A + P PNLLN SEKDIHIVT+DGHE
Sbjct: 127 KRVAFCSESPLCYSSSPGQPQWVYYKVVIELNQLAALRPCPNLLNTSEKDIHIVTKDGHE 186

Query: 186 FWFMGFICFSRALKSLNEALKHAHA 209
           FWF+GF+ FSRALKSLNEALKH+ A
Sbjct: 187 FWFLGFLLFSRALKSLNEALKHSCA 211

BLAST of Moc06g36630 vs. ExPASy TrEMBL
Match: A0A1S3CBY7 (GEM-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103499227 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.0e-82
Identity = 150/198 (75.76%), Postives = 174/198 (87.88%), Query Frame = 0

Query: 10  NPYIQYC--ASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDA 69
           NPYIQ+   +S   QPGK+D++  ALGRCGKMLE YGK+A EAA++V HHIKVSPSIGD 
Sbjct: 5   NPYIQHTPFSSPSPQPGKRDRVWDALGRCGKMLEGYGKMAGEAAENVWHHIKVSPSIGDV 64

Query: 70  AKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVA 129
           AKAR +QGTKL+ EGGPE+LF HTFGV+PG+KYLHSYACYL+T +GPVNGTLYISTKR+A
Sbjct: 65  AKARFVQGTKLLAEGGPERLFHHTFGVVPGEKYLHSYACYLATPTGPVNGTLYISTKRLA 124

Query: 130 FGSESPLCYSSS-PGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWF 189
           F SESPL YSSS PGQ EW+YYKVVIELN+VA   PSPNLL+PSEKDIH+VT+DGHEFWF
Sbjct: 125 FCSESPLYYSSSPPGQSEWLYYKVVIELNRVANFRPSPNLLDPSEKDIHLVTKDGHEFWF 184

Query: 190 MGFICFSRALKSLNEALK 205
           +GF+ FSRALK+LNEALK
Sbjct: 185 LGFLSFSRALKNLNEALK 202

BLAST of Moc06g36630 vs. ExPASy TrEMBL
Match: A0A0A0LCF9 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G634340 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 4.6e-80
Identity = 146/198 (73.74%), Postives = 173/198 (87.37%), Query Frame = 0

Query: 10  NPYIQY--CASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDA 69
           NPYIQ+   +S+  QP K+D++  ALGRCGK+LE YGK+A EAA++V HHIKVSPSIGD 
Sbjct: 5   NPYIQHTPSSSSSPQPPKRDRMWDALGRCGKILEGYGKMAGEAAENVWHHIKVSPSIGDV 64

Query: 70  AKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVA 129
           AKAR +QGTKL+ EGGPE+LF HTFGVIP +KYLHSYACYL+T +GPVNGTLYI+TKR+A
Sbjct: 65  AKARFVQGTKLLAEGGPERLFHHTFGVIPEEKYLHSYACYLATPTGPVNGTLYITTKRLA 124

Query: 130 FGSESPL-CYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWF 189
           F SESPL C SS PGQ EW+YYKVVIELN+VA + PSPNLL+PSEKDIH+VT+DGHEFWF
Sbjct: 125 FCSESPLYCSSSPPGQSEWLYYKVVIELNRVANLRPSPNLLDPSEKDIHLVTKDGHEFWF 184

Query: 190 MGFICFSRALKSLNEALK 205
           +GF+ FSRALK+LNEALK
Sbjct: 185 LGFLSFSRALKNLNEALK 202

BLAST of Moc06g36630 vs. TAIR 10
Match: AT1G28200.1 (FH interacting protein 1 )

HSP 1 Score: 193.0 bits (489), Expect = 2.4e-49
Identity = 90/195 (46.15%), Postives = 131/195 (67.18%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAAK 69
           NPY+   + +PA     D ++  LG+ GKM  +  K AE+ A +   H+K  PS+ DAA 
Sbjct: 65  NPYV---SPSPAPRNTMDSVKDTLGKWGKMAADATKKAEDLAGNFWQHLKTGPSVADAAV 124

Query: 70  ARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAFG 129
           +R+ QGTK++ EGG EK+F+ TF  +P +K L +YACYLSTS+GPV G +Y+ST ++AF 
Sbjct: 125 SRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKTYACYLSTSAGPVLGVMYLSTHKLAFS 184

Query: 130 SESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFMGF 189
           S++PL Y     Q  W YYKVV+  NQ+  +NPS + +N S+K I +++ D HEFWFMGF
Sbjct: 185 SDNPLSYKEGE-QTLWSYYKVVLPANQLKAVNPSTSRVNTSDKYIQVISIDNHEFWFMGF 244

Query: 190 ICFSRALKSLNEALK 205
           + +  A+KSL EA++
Sbjct: 245 VTYESAVKSLQEAVQ 255

BLAST of Moc06g36630 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.7e-48
Identity = 94/196 (47.96%), Postives = 130/196 (66.33%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGK-KDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAA 69
           NPY+   +   A   + KDK+   L RCGK +E+  + AE     +  H+K SPSI DAA
Sbjct: 29  NPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAA 88

Query: 70  KARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAF 129
            ARL QGTK++ EGGPE++FQ  FGV+  +K L S+ CY+ST+SGPV G +YIS +R+AF
Sbjct: 89  MARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAF 148

Query: 130 GSESPL-CYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFM 189
            S+  +   SS+ G     YYKVV+E  +++ I+ S N+L PSE+ +H+VTRDG EFWFM
Sbjct: 149 CSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFM 208

Query: 190 GFICFSRALKSLNEAL 204
           GF+ +  A   LN+AL
Sbjct: 209 GFVSYIDAFNCLNKAL 224

BLAST of Moc06g36630 vs. TAIR 10
Match: AT2G22475.1 (GRAM domain family protein )

HSP 1 Score: 185.3 bits (469), Expect = 5.1e-47
Identity = 89/198 (44.95%), Postives = 130/198 (65.66%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGKKD---KIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGD 69
           NPYI    +  +    KD    ++G LGR GK + E  K  E  A +   H++ +PS  D
Sbjct: 96  NPYIARSPAETSDASLKDTMETVKGVLGRWGKRVAEAAKKTESLAGNTWQHLRTAPSFAD 155

Query: 70  AAKARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRV 129
           AA  R+ Q TK+  EGG EK+F+ TF   P ++ L+S+ACYLSTS+GPV G LYIS+ ++
Sbjct: 156 AAMGRIAQSTKVFAEGGYEKIFRQTFETDPEEQLLNSFACYLSTSAGPVMGVLYISSAKL 215

Query: 130 AFGSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWF 189
           A+ S++PL Y +   Q EW YYKVVI L+Q+  +NPS +++NP+EK I +++ D HEFWF
Sbjct: 216 AYCSDNPLSYKNG-DQTEWSYYKVVIPLHQLKAVNPSASIVNPAEKYIQVISVDNHEFWF 275

Query: 190 MGFICFSRALKSLNEALK 205
           MGF+ +  A+ SL ++L+
Sbjct: 276 MGFLNYDGAVTSLQDSLQ 292

BLAST of Moc06g36630 vs. TAIR 10
Match: AT4G01600.2 (GRAM domain family protein )

HSP 1 Score: 183.0 bits (463), Expect = 2.5e-46
Identity = 94/196 (47.96%), Postives = 129/196 (65.82%), Query Frame = 0

Query: 10  NPYIQYCASAPAQPGK-KDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAA 69
           NPY+   +   A   + KDK+   L RCGK +E+  + AE      L  +K SPSI DAA
Sbjct: 29  NPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAE-----ALVGVKFSPSISDAA 88

Query: 70  KARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAF 129
            ARL QGTK++ EGGPE++FQ  FGV+  +K L S+ CY+ST+SGPV G +YIS +R+AF
Sbjct: 89  MARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAF 148

Query: 130 GSESPL-CYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFM 189
            S+  +   SS+ G     YYKVV+E  +++ I+ S N+L PSE+ +H+VTRDG EFWFM
Sbjct: 149 CSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFM 208

Query: 190 GFICFSRALKSLNEAL 204
           GF+ +  A   LN+AL
Sbjct: 209 GFVSYIDAFNCLNKAL 219

BLAST of Moc06g36630 vs. TAIR 10
Match: AT5G13200.1 (GRAM domain family protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.5e-35
Identity = 73/191 (38.22%), Postives = 110/191 (57.59%), Query Frame = 0

Query: 9   HNPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAA 68
           + PY+ Y  S    P   + +   +G    M   + + AE  A ++ H++K  PS+ + A
Sbjct: 72  YQPYVIY--SPVEHPTTNNPLEPVIG----MFHTWSRKAETVARNLWHNLKTGPSMSETA 131

Query: 69  KARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAF 128
             ++    K +T+GG E LF+  FG  P +    ++ACYLST++GPV GT+Y+S  RVAF
Sbjct: 132 WGKVNLTAKAITKGGFESLFRQIFGTEPNETLKKTFACYLSTTTGPVAGTVYLSNARVAF 191

Query: 129 GSESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFMG 188
            S+ PL +++  GQ  W YY+VV+ L  VA +NP      P EK I + T DGH+FWFMG
Sbjct: 192 CSDRPLYFTAPSGQESWSYYRVVVPLANVATVNPVVVKETPPEKYIQLTTVDGHDFWFMG 251

Query: 189 FICFSRALKSL 200
           F+ + +A   L
Sbjct: 252 FVNYEKATHHL 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156241.18.0e-119100.00GEM-like protein 1 [Momordica charantia][more]
XP_023553639.17.8e-9078.05GEM-like protein 1 [Cucurbita pepo subsp. pepo][more]
XP_022967332.11.3e-8977.56GEM-like protein 2 [Cucurbita maxima][more]
XP_022963576.11.1e-8877.07GEM-like protein 1 [Cucurbita moschata][more]
KAG6571599.11.5e-8876.59GEM-like protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9SE963.4e-4846.15GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1[more]
Q9M1223.8e-4747.96GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1[more]
Q8S8F87.2e-4644.95GLABRA2 expression modulator OS=Arabidopsis thaliana OX=3702 GN=GEM PE=1 SV=1[more]
Q9LYV62.2e-3438.22GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1[more]
Q9M0631.7e-3137.37Putative GEM-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g40100 PE=3 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1DUB73.9e-119100.00GEM-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111023171 PE=3 SV=1[more]
A0A6J1HQI26.4e-9077.56GEM-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111466886 PE=3 SV=1[more]
A0A6J1HGG75.5e-8977.07GEM-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111463872 PE=3 SV=1[more]
A0A1S3CBY71.0e-8275.76GEM-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103499227 PE=3 SV=1[more]
A0A0A0LCF94.6e-8073.74GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G634340 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G28200.12.4e-4946.15FH interacting protein 1 [more]
AT4G01600.12.7e-4847.96GRAM domain family protein [more]
AT2G22475.15.1e-4744.95GRAM domain family protein [more]
AT4G01600.22.5e-4647.96GRAM domain family protein [more]
AT5G13200.11.5e-3538.22GRAM domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 86..164
e-value: 1.7E-15
score: 67.5
IPR004182GRAM domainPFAMPF02893GRAMcoord: 86..208
e-value: 5.4E-20
score: 71.4
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 84..205
e-value: 6.0E-19
score: 69.9
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 8..207
NoneNo IPR availablePANTHERPTHR31969:SF9GEM-LIKE PROTEIN 2coord: 8..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g36630.1Moc06g36630.1mRNA