Moc06g35740 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g35740
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionaluminum-activated malate transporter 12-like
Locationchr6: 27451356 .. 27455576 (-)
RNA-Seq ExpressionMoc06g35740
SyntenyMoc06g35740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAATCAGATCATCAGAAGTTGAAAGTGAACAAAAGCCTCCAAATGAGCATGGACAAAATCAAACACTTGCCTGTTTTGTGTTGGAAGACCTCCAAGAAAGTGGGAGCTGAAGATCCCAGAAGAATTGTCCACTCTCTCAAAGTTGGTCTCTCCCTCACTCTGGTCTCTCTCTTGTACCTGGTGGAGCCATTGTTCAAAGGCATTGGAACCAATGCCATATGGGCCGTCATGACCGTCGTCGTCGTTCTAGAGTTCACCGCGGGTTCGTTCTCCACTTTAAAAAGTCGTGATCTTTAAAATCTCCGTTGTGAAAGCGAAAATTTCTGGCTCCGCCCCTGATTCTTGACATGGTTTGATGGCAGGAGCGACTTTGTGCAAAGGGCTCAATAGAGGGCTGGGGACATTGTTGGCAGGATCCTTGGCATTTCTCATTGAGGGTGTTGCCAATAGATCTGGAAGAGTTTTTAGAGCTTGTTTCATTGGAGCTGCAGTTTTTCTTATAGGTATCTTATTTATTTCATTTTCTTCCATATATCTTCTTGAAGTTCATTTTGTAATTTTGCCTTTTGTTTGATAAGAAAGTTTTTGAGATTTAGTTTTCCATTGTGCATAGCATTATAGTTCCTAAAATTTTGAGATGATAGTAAGATTTTTATGTAGTGGATGTGACTAACACTAAAAACTAATGATATTCTGACAGCTCTATTGAACAAATATTATATTGATTTATTATTTAACGTGATCTCTAGCAGTAGATGACGTACATATATAATATAACTTACTCTACTTTGAGGTAAGGTATCAAATCTCTTTTAGAGACCGTACCACTCGATCGTTGTCTAGTTGTTCATTTACATTTTGTCATTTGTTTGTCACGTTATGTCAAATTAGTCATTGTTTACCTAACAGACATATCATATCATTATTTAGTTGATTGTGCTAATGACAAAAACTTCGTTGAATGAGGAACGTAATTAGAGCTTTCTACATGCTCAAACTTTAACCTTTTTTTATATCTGTTACAGGAGGTGCAGCAACATATATGAGATTCTTTCCCAAAATAAAGAAGAACTATGATTATGGAGTGGTGATATTTCTCTTGACATTCAATCTGATAACCATATCGAGCTATCGCGTTGACAACGTGTTGAAAATCGCACACGACAGATTCTACACCATTGCCATTGGATGTGGAGTCTGCCTTCTGATGAGCCTACTGATCTTCCCAAACTGGTCTGGAGAAGAGCTCCACAATTCAACTGTGCTCAAGCTTGAAGGCCTTGCCAAATCCATTGAAGGTACACAGACAAAAGACCCTTTATTCAGTATCCTCCCATTATATATATATCCTTCTGTTTTTGTGCTATGAATGTAAGCGTTCGTTCATTACGTTTTCATTTTCTGTTTTTTGTTTCTCATTTTTTTATTACGTTGCAGCCTGTGTAAATGATTACTTCTTTGATGCTGAGATCGACGAAAACAAAGAGAGTTGTTCAAGAGACCAAATTTATAAAGGCTATAAGGCAGTTCTAGACTCAAAATCCACAGATGAAACTTTGGTGGGAAAGTCTTATTTGATATTCTTCTGAATGAGTTTAGCATGATGTGCATTTTTGATCCTATGTTTAATGTTTGTGTTGTAAACTGATCAGGCATTACAAGCAAGTTGGGAGCCAAGACACTCAAGTCACTGCTATAGAATCCCATGGCAACAGTATGTTAAATTAGGCGGCGTTCTTCGGCATTTCGGGTACACGGTCGTCGCTCTACACGGCTGTTTGCAGACCGAGATTCAGGTACAGATTGGTTCAAATCCTCTGTTTTCTCTGTTTTGTAGACAATCTAAGAGTTTGGTTGTTGGTTGTTCTGCTTTTGCCTCAAAAGACTCCGCGATCGGTTAGAGTCCTGTTCAAAGATCCTTGCAGCCGAGTAGCACGAGAAGTATCGAAAGCATTGATCGAGCTCGCAAACAGCATAAGGAACCGGCGGCATTGCTCACCGGAGATCCTGTCGGACCATCTTCACGAGGCCTTACAAGACCTGAACAAGGCGATAAAATCACAGCCGCGACTCTTCCTAGGCTCGAACAGGAACCAAGCCAGAAACATGCTAGCATTGGCAGCAGCACAAGCAGGGCAAAAACAAGAGGAAAAGGAAAAGGAAAAGCGGTCCGGGGTATCGTTGTCGAGCGTGAAAACGGACTCGTCGGCGCTGATGGAATGGAGGACGAAAAGAAGGGAGGCTGAGAGGAAGGTGTTGAGGCCACAGCTAAGCAAGATAGCAATCACAAGCCTTGAGTTCTCAGAAGCACTTCCATTTGCTGCTTTTGCTTCTCTGCTGGTTGAGACTGTGGCAAAGCTTGACATTCTGATAGATGAAGTTGAAGAGTTGGGGAGAATTGCTTGCTTCAAAGAGTTCAAAAATGGTGATGATGAACAACATATAACTGTCAAATGTGAGAAGCCAAAGATCAGTGTGTCTCAGAGTCAGCTGTCTTCAGTTAGTGCTGCAGAATGAGGGGATTGGTTTTGTTGCTGAAAATGGACTTTAAGAGGATTATTTCGGAGGGTGTGTTTTGTTTGATCATATGATTATGTGCAGGAATGGGGATTGCTTTTGGTCTTCGAATATTGGAATAAACAAAAAAAGAAAAACTCAGTTCTGTTCTACCTAGACTTTGCTACATTTTCCAAATCATGGCCTACCATTGCAAACAACTAAACTTTAAGATTTCTTTTTTCTTTTTAATTTAAAGTTTTTAGAAGATCATTACAAAAGCTAGGACAATTCAATGTTAAAGTAGGTTGGAGGTTTGATCTCCCACCTCTAGGTTGATATTTCTACACTAGTATGATTTTGCCCACTTTTAAGCCTAAGTTCGTATGATTTTGTTTTTTTTTTTTTTTTTACTTCAGGCTGGAAATGTTCCTACTTTATACCATATTGTCCTTATCTATATATCTTAGATATTTTTCACTTCTAATTGATATGGAACTATGTTTCATCTTTACAAAAGCAAAAAATAAACCAACCCGAATTTAACTCAATTATCAAGACATTAATCACATTTCCTAAAGTTTGAAAGTTCAAATCTTCTATCGCATATTTGTGTAATGACTCGAATGCCCTAACTTCTCAAAAATTGAAAACATTAGTTAGTAATTTTTCGGGGGTTAGAAGTTAAAAAGTGGGTTAAATTATAAGTATAGTTCTTGAACTTTGAAAAAGTGTCTAGTAGATTTCTAAACTTTCAAATTTGTGTCTAATAAGTCCTTCAACTTTAAAAGTATCTAAAATAGTCTCATAAACTTGTAGTTTTGTATCTTGTAAATTGTTTATGCAAAAAATGTGACGGGGTATGACCTGGTCAAGGTCGGCCATGCAGAAGAGCTCGACCTGCTCCAGGTATGACCGCGGGGAGAGCTTGACCTGCTTCAAGTATGACCGTGGGAAGAGCTTGGGTATCCTTGGAAAGCAAGGGATGACCAACTTGAAGGGTCAAGGTCGGCTGACTGGGATGAGCTAGTGTCAACAATGAGCTGAAAGGCCACTTGGCGAGGAAAGACAAGGGAACAAACACTCTGACGCTCAAGTCAGTTTTGAGTCCGACCTTAGTATGGAGACCGAGCTAGAATACGATACTTGACTGAAGAGTAAAGTGTCCTATTTATAGGTTGAGGTACCCGACAAAGAATCCGACTAGCGATTGAGCATGTCGGGACCAAGGTCGGCCTGTCGGGAGTAGCTATTCCAGTTCATACTCTTGTCATCTGGAATTTGACTAAGTATGGAAGGTCGTTCCTCCCTTGAAATGATCTTCAGGGAAGACCTTCAGATCGGCCTTTAGGGAAGGCCTCAGGTCGGCCATTAGGCAAGGCCTTCAGGTCGGCCCTCAGGGGAGCTCTTCAGGTCGGCCATCAGGCAAGGCCTTCAAGTCGGCCTTTAGGGGAGATCTTCTTGTTGGTCCTCAGTAGAGCTCTTCAGGTCGACCCTCAGTGGAGCTCTTTAGGTCGGCCACTTCAGGTCGGCCAACAAAGGAAAGACCTCCAAAGGCGATCTACAGCAGCGGCGGCACGATTTCCTCTGGCGACGTGTTCTCCGGCGAACGGCCCAGCGTTCTCCATGAACCCCGGCGGTCGAAACTCTCGCCGGTGGGGATGGGACGCTCGAGTTCGAGGCCGACCTGCAGAGGAGGGAGTTTGCCGCACCCACTGA

mRNA sequence

ATGGCAGAATCAGATCATCAGAAGTTGAAAGTGAACAAAAGCCTCCAAATGAGCATGGACAAAATCAAACACTTGCCTGTTTTGTGTTGGAAGACCTCCAAGAAAGTGGGAGCTGAAGATCCCAGAAGAATTGTCCACTCTCTCAAAGTTGGTCTCTCCCTCACTCTGGTCTCTCTCTTGTACCTGGTGGAGCCATTGTTCAAAGGCATTGGAACCAATGCCATATGGGCCGTCATGACCGTCGTCGTCGTTCTAGAGTTCACCGCGGGAGCGACTTTGTGCAAAGGGCTCAATAGAGGGCTGGGGACATTGTTGGCAGGATCCTTGGCATTTCTCATTGAGGGTGTTGCCAATAGATCTGGAAGAGTTTTTAGAGCTTGTTTCATTGGAGCTGCAGTTTTTCTTATAGGAGGTGCAGCAACATATATGAGATTCTTTCCCAAAATAAAGAAGAACTATGATTATGGAGTGGTGATATTTCTCTTGACATTCAATCTGATAACCATATCGAGCTATCGCGTTGACAACGTGTTGAAAATCGCACACGACAGATTCTACACCATTGCCATTGGATGTGGAGTCTGCCTTCTGATGAGCCTACTGATCTTCCCAAACTGGTCTGGAGAAGAGCTCCACAATTCAACTGTGCTCAAGCTTGAAGGCCTTGCCAAATCCATTGAAGCCTGTGTAAATGATTACTTCTTTGATGCTGAGATCGACGAAAACAAAGAGAGTTGTTCAAGAGACCAAATTTATAAAGGCTATAAGGCAGTTCTAGACTCAAAATCCACAGATGAAACTTTGGCATTACAAGCAAGTTGGGAGCCAAGACACTCAAGTCACTGCTATAGAATCCCATGGCAACAGTATGTTAAATTAGGCGGCGTTCTTCGGCATTTCGGGTACACGGTCGTCGCTCTACACGGCTGTTTGCAGACCGAGATTCAGACTCCGCGATCGGTTAGAGTCCTGTTCAAAGATCCTTGCAGCCGAGTAGCACGAGAAGTATCGAAAGCATTGATCGAGCTCGCAAACAGCATAAGGAACCGGCGGCATTGCTCACCGGAGATCCTGTCGGACCATCTTCACGAGGCCTTACAAGACCTGAACAAGGCGATAAAATCACAGCCGCGACTCTTCCTAGGCTCGAACAGGAACCAAGCCAGAAACATGCTAGCATTGGCAGCAGCACAAGCAGGGCAAAAACAAGAGGAAAAGGAAAAGGAAAAGCGGTCCGGGGTATCGTTGTCGAGCGTGAAAACGGACTCGTCGGCGCTGATGGAATGGAGGACGAAAAGAAGGGAGGCTGAGAGGAAGGTGTTGAGGCCACAGCTAAGCAAGATAGCAATCACAAGCCTTGAGTTCTCAGAAGCACTTCCATTTGCTGCTTTTGCTTCTCTGCTGGTTGAGACTGTGGCAAAGCTTGACATTCTGATAGATGAAGTTGAAGAGTTGGGGAGAATTGCTTGCTTCAAAGAGTTCAAAAATGGTGATGATGAACAACATATAACTGTCAAATGTGAGAAGCCAAAGATCAGTGTGTCTCAGAGTCAGCTGTCTTCAGTCGGCCATGCAGAAGAGCTCGACCTGCTCCAGGTTGAGGGAAGACCTTCAGATCGGCCTTTAGGGAAGGCCTCAGGTCGGCCATTAGGCAAGGCCTTCAGGTCGGCCCTCAGGGGAGCTCTTCAGGTCGGCCATCAGGCAAGGCCTTCAAGTCGGCCTTTAGGGGAGATCTTCTTGTTGGTCCTCAGTAGAGCTCTTCAGGTCGACCCTCAGTGGAGCTCTTTAGGTCGGCCACTTCAGGTCGGCCAACAAAGGAAAGACCTCCAAAGGCGATCTACAGCAGCGGCGGCACGATTTCCTCTGGCGACGTGTTCTCCGGCGAACGGCCCAGCGTTCTCCATGAACCCCGGCGGTCGAAACTCTCGCCGGTGGGGATGGGACGCTCGAGTTCGAGGCCGACCTGCAGAGGAGGGAGTTTGCCGCACCCACTGA

Coding sequence (CDS)

ATGGCAGAATCAGATCATCAGAAGTTGAAAGTGAACAAAAGCCTCCAAATGAGCATGGACAAAATCAAACACTTGCCTGTTTTGTGTTGGAAGACCTCCAAGAAAGTGGGAGCTGAAGATCCCAGAAGAATTGTCCACTCTCTCAAAGTTGGTCTCTCCCTCACTCTGGTCTCTCTCTTGTACCTGGTGGAGCCATTGTTCAAAGGCATTGGAACCAATGCCATATGGGCCGTCATGACCGTCGTCGTCGTTCTAGAGTTCACCGCGGGAGCGACTTTGTGCAAAGGGCTCAATAGAGGGCTGGGGACATTGTTGGCAGGATCCTTGGCATTTCTCATTGAGGGTGTTGCCAATAGATCTGGAAGAGTTTTTAGAGCTTGTTTCATTGGAGCTGCAGTTTTTCTTATAGGAGGTGCAGCAACATATATGAGATTCTTTCCCAAAATAAAGAAGAACTATGATTATGGAGTGGTGATATTTCTCTTGACATTCAATCTGATAACCATATCGAGCTATCGCGTTGACAACGTGTTGAAAATCGCACACGACAGATTCTACACCATTGCCATTGGATGTGGAGTCTGCCTTCTGATGAGCCTACTGATCTTCCCAAACTGGTCTGGAGAAGAGCTCCACAATTCAACTGTGCTCAAGCTTGAAGGCCTTGCCAAATCCATTGAAGCCTGTGTAAATGATTACTTCTTTGATGCTGAGATCGACGAAAACAAAGAGAGTTGTTCAAGAGACCAAATTTATAAAGGCTATAAGGCAGTTCTAGACTCAAAATCCACAGATGAAACTTTGGCATTACAAGCAAGTTGGGAGCCAAGACACTCAAGTCACTGCTATAGAATCCCATGGCAACAGTATGTTAAATTAGGCGGCGTTCTTCGGCATTTCGGGTACACGGTCGTCGCTCTACACGGCTGTTTGCAGACCGAGATTCAGACTCCGCGATCGGTTAGAGTCCTGTTCAAAGATCCTTGCAGCCGAGTAGCACGAGAAGTATCGAAAGCATTGATCGAGCTCGCAAACAGCATAAGGAACCGGCGGCATTGCTCACCGGAGATCCTGTCGGACCATCTTCACGAGGCCTTACAAGACCTGAACAAGGCGATAAAATCACAGCCGCGACTCTTCCTAGGCTCGAACAGGAACCAAGCCAGAAACATGCTAGCATTGGCAGCAGCACAAGCAGGGCAAAAACAAGAGGAAAAGGAAAAGGAAAAGCGGTCCGGGGTATCGTTGTCGAGCGTGAAAACGGACTCGTCGGCGCTGATGGAATGGAGGACGAAAAGAAGGGAGGCTGAGAGGAAGGTGTTGAGGCCACAGCTAAGCAAGATAGCAATCACAAGCCTTGAGTTCTCAGAAGCACTTCCATTTGCTGCTTTTGCTTCTCTGCTGGTTGAGACTGTGGCAAAGCTTGACATTCTGATAGATGAAGTTGAAGAGTTGGGGAGAATTGCTTGCTTCAAAGAGTTCAAAAATGGTGATGATGAACAACATATAACTGTCAAATGTGAGAAGCCAAAGATCAGTGTGTCTCAGAGTCAGCTGTCTTCAGTCGGCCATGCAGAAGAGCTCGACCTGCTCCAGGTTGAGGGAAGACCTTCAGATCGGCCTTTAGGGAAGGCCTCAGGTCGGCCATTAGGCAAGGCCTTCAGGTCGGCCCTCAGGGGAGCTCTTCAGGTCGGCCATCAGGCAAGGCCTTCAAGTCGGCCTTTAGGGGAGATCTTCTTGTTGGTCCTCAGTAGAGCTCTTCAGGTCGACCCTCAGTGGAGCTCTTTAGGTCGGCCACTTCAGGTCGGCCAACAAAGGAAAGACCTCCAAAGGCGATCTACAGCAGCGGCGGCACGATTTCCTCTGGCGACGTGTTCTCCGGCGAACGGCCCAGCGTTCTCCATGAACCCCGGCGGTCGAAACTCTCGCCGGTGGGGATGGGACGCTCGAGTTCGAGGCCGACCTGCAGAGGAGGGAGTTTGCCGCACCCACTGA

Protein sequence

MAESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILIDEVEELGRIACFKEFKNGDDEQHITVKCEKPKISVSQSQLSSVGHAEELDLLQVEGRPSDRPLGKASGRPLGKAFRSALRGALQVGHQARPSSRPLGEIFLLVLSRALQVDPQWSSLGRPLQVGQQRKDLQRRSTAAAARFPLATCSPANGPAFSMNPGGRNSRRWGWDARVRGRPAEEGVCRTH
Homology
BLAST of Moc06g35740 vs. NCBI nr
Match: XP_022156876.1 (aluminum-activated malate transporter 12-like [Momordica charantia])

HSP 1 Score: 1013.4 bits (2619), Expect = 8.6e-292
Identity = 524/526 (99.62%), Postives = 524/526 (99.62%), Query Frame = 0

Query: 1   MAESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLL 60
           MAESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLL
Sbjct: 1   MAESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLL 60

Query: 61  YLVEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRS 120
           YLVEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRS
Sbjct: 61  YLVEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRS 120

Query: 121 GRVFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKI 180
           GRVFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKI
Sbjct: 121 GRVFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKI 180

Query: 181 AHDRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID 240
           AHDRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID
Sbjct: 181 AHDRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID 240

Query: 241 ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHF 300
           ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHF
Sbjct: 241 ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHF 300

Query: 301 GYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSD 360
           GYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSD
Sbjct: 301 GYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSD 360

Query: 361 HLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVK 420
           HLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVK
Sbjct: 361 HLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVK 420

Query: 421 TDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILID 480
           TDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILID
Sbjct: 421 TDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILID 480

Query: 481 EVEELGRIACFKEFKNGDDEQHITVKCEKPKISVSQSQLSSVGHAE 527
           EVEELGRIACFKEFKNGDDEQHITVKCEKPKISVSQSQLSSV  AE
Sbjct: 481 EVEELGRIACFKEFKNGDDEQHITVKCEKPKISVSQSQLSSVSAAE 526

BLAST of Moc06g35740 vs. NCBI nr
Match: XP_038888238.1 (aluminum-activated malate transporter 12-like [Benincasa hispida])

HSP 1 Score: 931.8 bits (2407), Expect = 3.3e-267
Identity = 479/528 (90.72%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 5   DHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVE 64
           +HQK+++ K LQ++M+KIK  P+LCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL++
Sbjct: 19  NHQKMQMKKHLQVTMNKIKQFPILCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIQ 78

Query: 65  PLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVF 124
           PLFKGIG+NA+WAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAF IEGVANR+G+VF
Sbjct: 79  PLFKGIGSNALWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFFIEGVANRTGKVF 138

Query: 125 RACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDR 184
           RACFIGAAVFLIG AATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDR
Sbjct: 139 RACFIGAAVFLIGSAATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDR 198

Query: 185 FYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKE 244
            YTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVN+YFFD EIDENKE
Sbjct: 199 LYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNEYFFDTEIDENKE 258

Query: 245 SCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTV 304
           SCS DQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYR PWQQYVKLGGVLRHFGYTV
Sbjct: 259 SCSGDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRFPWQQYVKLGGVLRHFGYTV 318

Query: 305 VALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHE 364
           VALHGCLQTEIQTPRSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHE
Sbjct: 319 VALHGCLQTEIQTPRSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHE 378

Query: 365 ALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSS 424
           ALQDLNKAIKSQPRLFLGSN+NQARNMLALAAAQAGQKQ EK   ++ GVSLSSVKTDSS
Sbjct: 379 ALQDLNKAIKSQPRLFLGSNKNQARNMLALAAAQAGQKQNEK---RQLGVSLSSVKTDSS 438

Query: 425 ALMEWRTKR-----REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILI 484
           ALMEW+TKR     REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDI+I
Sbjct: 439 ALMEWKTKRVSEQSREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDIVI 498

Query: 485 DEVEELGRIACFKEFKNGDD-EQHITVKCEKPKISVSQSQLSSVGHAE 527
           DEVEELGRIACFKEFKNGDD E+HITVKCEKPKISVSQ+QLSSV  AE
Sbjct: 499 DEVEELGRIACFKEFKNGDDEEEHITVKCEKPKISVSQNQLSSVSGAE 543

BLAST of Moc06g35740 vs. NCBI nr
Match: XP_004148510.2 (aluminum-activated malate transporter 12 [Cucumis sativus] >KAE8651447.1 hypothetical protein Csa_002311 [Cucumis sativus])

HSP 1 Score: 928.3 bits (2398), Expect = 3.7e-266
Identity = 475/530 (89.62%), Postives = 507/530 (95.66%), Query Frame = 0

Query: 5   DHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVE 64
           +HQ + + K LQ++M+KIKH P+LCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL++
Sbjct: 4   NHQNILMKKHLQVTMNKIKHFPILCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIQ 63

Query: 65  PLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVF 124
           PLF+GIG NA+WAVMTVVVVLEFTAGATLCKGLNRGLGT+LAGSLAF IEGVANR+G+VF
Sbjct: 64  PLFQGIGNNALWAVMTVVVVLEFTAGATLCKGLNRGLGTVLAGSLAFFIEGVANRTGKVF 123

Query: 125 RACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDR 184
           RACFIGAAVFLIG  ATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDR
Sbjct: 124 RACFIGAAVFLIGSVATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDR 183

Query: 185 FYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKE 244
           FYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVN+YFFD EIDENKE
Sbjct: 184 FYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNEYFFDTEIDENKE 243

Query: 245 SCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTV 304
           SCS DQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTV
Sbjct: 244 SCSGDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTV 303

Query: 305 VALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHE 364
           VALHGCLQTEIQTPRSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHE
Sbjct: 304 VALHGCLQTEIQTPRSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHE 363

Query: 365 ALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSS 424
           ALQDLNKAIKSQPRLFLGSN+NQ+RNMLALAAA+AGQKQ  KEK+++SGVSLSSVKTDSS
Sbjct: 364 ALQDLNKAIKSQPRLFLGSNKNQSRNMLALAAAEAGQKQ--KEKKRQSGVSLSSVKTDSS 423

Query: 425 ALMEWRTKR-----REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILI 484
           ALMEW+TKR     REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDI+I
Sbjct: 424 ALMEWKTKRASEQSREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDIVI 483

Query: 485 DEVEELGRIACFKEFKNGDD---EQHITVKCEKPKISVSQSQLSSVGHAE 527
           DEVEELGRIACFKEFK+GDD   E+HITVKCEKPKI+V+Q+QLSSV  AE
Sbjct: 484 DEVEELGRIACFKEFKHGDDDDKEEHITVKCEKPKINVTQNQLSSVSGAE 531

BLAST of Moc06g35740 vs. NCBI nr
Match: XP_008466647.2 (PREDICTED: aluminum-activated malate transporter 12-like [Cucumis melo] >KAA0038551.1 aluminum-activated malate transporter 12-like [Cucumis melo var. makuwa] >TYK31147.1 aluminum-activated malate transporter 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 911.4 bits (2354), Expect = 4.6e-261
Identity = 468/522 (89.66%), Postives = 496/522 (95.02%), Query Frame = 0

Query: 19  MDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAV 78
           M+KIKH PVLCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL++PLF+GIG+NA+WAV
Sbjct: 1   MNKIKHFPVLCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIQPLFEGIGSNALWAV 60

Query: 79  MTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGG 138
           MTVVVVLEFTAGATLCKGLNRGLGT+LAGSLAF IEGVANR+G+VFRACFIGAAVFLIG 
Sbjct: 61  MTVVVVLEFTAGATLCKGLNRGLGTVLAGSLAFFIEGVANRTGKVFRACFIGAAVFLIGS 120

Query: 139 AATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLM 198
            ATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDRFYTIAIGCGVCLLM
Sbjct: 121 VATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDRFYTIAIGCGVCLLM 180

Query: 199 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAV 258
           SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVN+YFFD EIDENKESCS DQIYKGYKAV
Sbjct: 181 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNEYFFDTEIDENKESCSGDQIYKGYKAV 240

Query: 259 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 318
           LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP
Sbjct: 241 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 300

Query: 319 RSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 378
           RSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR
Sbjct: 301 RSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 360

Query: 379 LFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSSALMEWRTKR----- 438
           LFLGSN+NQ+RNMLALAAA+AGQKQ EK+++  SGVSLSSVKTDSSALMEW+TKR     
Sbjct: 361 LFLGSNKNQSRNMLALAAAEAGQKQNEKKRQ--SGVSLSSVKTDSSALMEWKTKRASEQS 420

Query: 439 REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILIDEVEELGRIACFKE 498
           REAERKVLR QLSKIAITSLEFSEALPFAAFASLLVETVAKLDI+IDEVEELGRIACFKE
Sbjct: 421 REAERKVLRTQLSKIAITSLEFSEALPFAAFASLLVETVAKLDIVIDEVEELGRIACFKE 480

Query: 499 FKNGDD---------EQHITVKCEKPKISVSQSQLSSVGHAE 527
           FK+GDD         E+HITVKCEKPK++V+Q+QLSSV  AE
Sbjct: 481 FKHGDDNDNDNDNDEEEHITVKCEKPKVNVTQNQLSSVSGAE 520

BLAST of Moc06g35740 vs. NCBI nr
Match: XP_022952267.1 (aluminum-activated malate transporter 12-like [Cucurbita moschata] >KAG6571842.1 Aluminum-activated malate transporter 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 910.2 bits (2351), Expect = 1.0e-260
Identity = 472/525 (89.90%), Postives = 494/525 (94.10%), Query Frame = 0

Query: 9   LKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFK 68
           ++VNK +Q++MDKI+H PVLCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL+EPLFK
Sbjct: 1   MQVNKHIQVTMDKIQHFPVLCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIEPLFK 60

Query: 69  GIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACF 128
            +G+NA WAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAF IEGVANRSGRVFRACF
Sbjct: 61  RLGSNATWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFFIEGVANRSGRVFRACF 120

Query: 129 IGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTI 188
           IGAAVFLIG AATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDR YTI
Sbjct: 121 IGAAVFLIGAAATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDRLYTI 180

Query: 189 AIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSR 248
           AIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIE CVN+YFFD EIDENKESCS 
Sbjct: 181 AIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEGCVNEYFFDTEIDENKESCSG 240

Query: 249 DQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALH 308
           DQIYKGYKAVLDSKS DE+LALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALH
Sbjct: 241 DQIYKGYKAVLDSKSIDESLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALH 300

Query: 309 GCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQD 368
           GCLQTEIQTPRSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQD
Sbjct: 301 GCLQTEIQTPRSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQD 360

Query: 369 LNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSSALME 428
           LNKAIKSQPRLFLGSNRN+A NMLALAAA+AGQK+       RSG SLSSVKTDSSALME
Sbjct: 361 LNKAIKSQPRLFLGSNRNKASNMLALAAAEAGQKR------CRSGGSLSSVKTDSSALME 420

Query: 429 WRTKR-----REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILIDEVE 488
           W+TKR     REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLD +IDEVE
Sbjct: 421 WKTKRASEQSREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDNVIDEVE 480

Query: 489 ELGRIACFKEFKNGDD--EQHITVKCEKPKISVSQSQLSSVGHAE 527
           ELGRIACFKEF NGDD  EQHITVKCEKP I+VSQ+QLSSVG AE
Sbjct: 481 ELGRIACFKEFINGDDHEEQHITVKCEKPNINVSQNQLSSVGGAE 519

BLAST of Moc06g35740 vs. ExPASy Swiss-Prot
Match: O49696 (Aluminum-activated malate transporter 12 OS=Arabidopsis thaliana OX=3702 GN=ALMT12 PE=2 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 3.6e-184
Identity = 351/529 (66.35%), Postives = 415/529 (78.45%), Query Frame = 0

Query: 20  DKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAVM 79
           +KIK +P   W     VG EDPRR++H+LKVGLSLTLVSLLYL+EPLFKGIG+NAIWAVM
Sbjct: 28  EKIKKIPKRLW----NVGKEDPRRVIHALKVGLSLTLVSLLYLMEPLFKGIGSNAIWAVM 87

Query: 80  TVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGGA 139
           TVVVVLEF+AGATLCKGLNRGLGTL+AGSLAF IE VAN SG+V RA FIG AVF+IG A
Sbjct: 88  TVVVVLEFSAGATLCKGLNRGLGTLIAGSLAFFIEFVANDSGKVLRAIFIGTAVFIIGAA 147

Query: 140 ATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLMS 199
           ATY+RF P IKKNYDYGVVIFLLTFNLIT+SSYRVD+V+ IAHDRFYTIA+GCG+CL MS
Sbjct: 148 ATYIRFIPYIKKNYDYGVVIFLLTFNLITVSSYRVDSVINIAHDRFYTIAVGCGICLFMS 207

Query: 200 LLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAVL 259
           LL+FP WSGE+LH +TV KL+GL++SIEACV++YF   E  E +++ S+D+IY+GY+AVL
Sbjct: 208 LLVFPIWSGEDLHKTTVGKLQGLSRSIEACVDEYF---EEKEKEKTDSKDRIYEGYQAVL 267

Query: 260 DSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTPR 319
           DSKSTDETLAL A+WEPRH+  C+R P QQYVK+G VLR FGYTVVALHGCLQTEIQTPR
Sbjct: 268 DSKSTDETLALYANWEPRHTLRCHRFPCQQYVKVGAVLRQFGYTVVALHGCLQTEIQTPR 327

Query: 320 SVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPRL 379
           SVR LFKDPC R+A EV KAL ELA+SI N RHCSPEILSDHLH ALQDLN AIKSQP+L
Sbjct: 328 SVRALFKDPCVRLAGEVCKALTELADSISNHRHCSPEILSDHLHVALQDLNSAIKSQPKL 387

Query: 380 FLGSNRNQARNM--------------------------LALAAAQAGQKQ--EEKEKEKR 439
           FLGSN ++  N                           ++L   + G ++  E   ++ +
Sbjct: 388 FLGSNLHRHNNKHQNGSISNNKHHQRNSSNSGKDLNGDVSLQNTETGTRKITETGSRQGQ 447

Query: 440 SG-VSLSSVKTDSSALMEWRTKRREA--------ERKVLRPQLSKIAI-TSLEFSEALPF 499
           +G VSLSS +TD+SALME+R   + +        ER++LRPQLSKIA+ TSLEFSEALPF
Sbjct: 448 NGAVSLSSFRTDTSALMEYRRSFKNSNSEMSAAGERRMLRPQLSKIAVMTSLEFSEALPF 507

Query: 500 AAFASLLVETVAKLDILIDEVEELGRIACFKEFKNGDDEQHITVKCEKP 511
           AAFASLLVE VA+LD +I+EVEELGRIA FKE+ N  D+    V+CE P
Sbjct: 508 AAFASLLVEMVARLDNVIEEVEELGRIASFKEYDNKRDQTADDVRCENP 549

BLAST of Moc06g35740 vs. ExPASy Swiss-Prot
Match: Q9LS22 (Aluminum-activated malate transporter 14 OS=Arabidopsis thaliana OX=3702 GN=ALMT14 PE=2 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.4e-175
Identity = 332/515 (64.47%), Postives = 392/515 (76.12%), Query Frame = 0

Query: 21  KIKHLPVLCWKTSK---KVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWA 80
           K+  LP    K  K   KVG +DPRR+ H+LKVG+SLTLVSLLYL+EPLFKGIG +AIWA
Sbjct: 24  KVLELPTKIKKILKNIWKVGKDDPRRVKHALKVGVSLTLVSLLYLMEPLFKGIGNSAIWA 83

Query: 81  VMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIG 140
           VMTVVVVLEF+AGATLCKGLNRGLGTL+AGSLAF IE VAN SG++FRA FIGAAVF+IG
Sbjct: 84  VMTVVVVLEFSAGATLCKGLNRGLGTLIAGSLAFFIEFVANDSGKIFRAIFIGAAVFIIG 143

Query: 141 GAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLL 200
              TY+RF P IKKNYDYG++IFLLTFNLIT+SSYRVD V+KIAH+RFYTIA+G G+CLL
Sbjct: 144 ALITYLRFIPYIKKNYDYGMLIFLLTFNLITVSSYRVDTVIKIAHERFYTIAMGVGICLL 203

Query: 201 MSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKA 260
           MSLL+FP WSGE+LH STV KL+GL+ SIEACVN+YF + E DE     S D IY GYK 
Sbjct: 204 MSLLVFPIWSGEDLHKSTVAKLQGLSYSIEACVNEYFEEEEKDEETSDLSEDTIYNGYKT 263

Query: 261 VLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQT 320
           VLDSKS DE LA+ ASWEPRH+ HC+R PW+ YVK+G VLR FGYTVVALHGCL+TEIQT
Sbjct: 264 VLDSKSADEALAMYASWEPRHTRHCHRFPWKHYVKVGSVLRQFGYTVVALHGCLKTEIQT 323

Query: 321 PRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQP 380
           PR +R LFKDPC R+A E+ K L ELA SIRNRRHCSPEILSD L  ALQDLN AIKSQP
Sbjct: 324 PRPLRGLFKDPCVRLAGEICKVLSELAASIRNRRHCSPEILSDSLQVALQDLNTAIKSQP 383

Query: 381 RLFLGSNR-------NQARNMLALAAAQ--------------AGQKQEEKEKEKRSGVSL 440
           +LFLGS++       N  R+   +A +Q               G  + E+       VS 
Sbjct: 384 KLFLGSSQNGNVSQGNSGRHNPNVAVSQHINKDTNEAASYQNTGTPRGERMSRFGPNVSF 443

Query: 441 SSVKTDSSALMEWRTKRREAERKVLRPQLSKIAI-TSLEFSEALPFAAFASLLVETVAKL 500
           S ++ D+   +E R+     ERK+LR QLS+I + TSLEFSEALPFAAFASLLVE VA+L
Sbjct: 444 SRLRADT---LERRSAAATNERKILRQQLSRIVVLTSLEFSEALPFAAFASLLVEMVARL 503

Query: 501 DILIDEVEELGRIACFKEFKNGDDEQHITVKCEKP 511
           D +I+EVEELG IACFK++ N  D++ + V+ EKP
Sbjct: 504 DNVIEEVEELGTIACFKDYDNNVDQKDVEVRVEKP 535

BLAST of Moc06g35740 vs. ExPASy Swiss-Prot
Match: Q9LS23 (Aluminum-activated malate transporter 13 OS=Arabidopsis thaliana OX=3702 GN=ALMT13 PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 5.7e-153
Identity = 302/518 (58.30%), Postives = 363/518 (70.08%), Query Frame = 0

Query: 3   ESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYL 62
           E   +K K   +L   M KI        +    VG EDPRR++H+LKVG++LTLVSLLYL
Sbjct: 17  EDSRKKRKKGLNLPKKMKKI-------LRNLWNVGKEDPRRVIHALKVGVALTLVSLLYL 76

Query: 63  VEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGR 122
           +EP F+G+G NA+WAVMTVVVVLEF+AGATL KGLNRGLGTL+AGSLAF IE VA  SG+
Sbjct: 77  MEPFFEGVGKNALWAVMTVVVVLEFSAGATLRKGLNRGLGTLIAGSLAFFIEWVAIHSGK 136

Query: 123 VFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAH 182
           +    FIG +VF IG   TYMRF P IKKNYDYG+++FLLTFNLIT+SSYRVD V+KIAH
Sbjct: 137 ILGGIFIGTSVFTIGSMITYMRFIPYIKKNYDYGMLVFLLTFNLITVSSYRVDTVIKIAH 196

Query: 183 DRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID-- 242
           +R YTI +G G+CL MSLL FP WSG++LH ST+ KL+GL++ IEACV++YF +   D  
Sbjct: 197 ERLYTIGMGIGICLFMSLLFFPIWSGDDLHKSTITKLQGLSRCIEACVSEYFEEKLKDNE 256

Query: 243 -ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRH 302
             + ES   D IY GY  VLDSKS DE LA+ A WEPRH+  C + P QQY+K+G VLR 
Sbjct: 257 TSDSESDDEDLIYNGYNTVLDSKSADEALAMYAKWEPRHTRRCNKFPSQQYIKVGSVLRK 316

Query: 303 FGYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILS 362
           FGYTVVALHGCLQTEIQTPRS+RVLFKDPC R+A E+ K L EL+ SI+NRRHCS EILS
Sbjct: 317 FGYTVVALHGCLQTEIQTPRSIRVLFKDPCVRLAGEICKVLSELSESIQNRRHCSSEILS 376

Query: 363 DHLHEALQDLNKAIKSQPRLFLGSN--RNQARNMLALAAAQAGQKQEE------------ 422
           D L  AL+DLN  IKSQP+LFLGSN   N     L    +   +                
Sbjct: 377 DSLEAALKDLNSTIKSQPKLFLGSNLHSNITNKHLNGHVSYYNETNSNGTVSYHNDNNTN 436

Query: 423 --------KEKEKRSGVSLSSVKTDSSALMEWRTKRREAERKVLRPQLSKIAI-TSLEFS 482
                   +E +  S + L+SV    S+L   +      E++ LR QLSKIA+  SLEFS
Sbjct: 437 GCVLGETIEENDTVSPLPLNSV-VSLSSLRSVKKSAATGEKRRLRKQLSKIAVMKSLEFS 496

Query: 483 EALPFAAFASLLVETVAKLDILIDEVEELGRIACFKEF 495
           EALPFAAFASLLVE VA+LD +IDEVEELG IACFKE+
Sbjct: 497 EALPFAAFASLLVEMVARLDTVIDEVEELGTIACFKEY 526

BLAST of Moc06g35740 vs. ExPASy Swiss-Prot
Match: O23086 (Aluminum-activated malate transporter 10 OS=Arabidopsis thaliana OX=3702 GN=ALMT10 PE=3 SV=2)

HSP 1 Score: 285.0 bits (728), Expect = 2.1e-75
Identity = 177/502 (35.26%), Postives = 273/502 (54.38%), Query Frame = 0

Query: 15  LQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNA 74
           +++ M+++       W    ++GA+DP ++VH LKVGL+L+LVS+ Y + PL+ G+G NA
Sbjct: 42  MKVIMERVAKFMRKAW----RIGADDPAKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNA 101

Query: 75  IWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVF 134
           +WA+MTVVVV E   GAT CK +NR + T+LAGSL   +  VA +SG+      IG +VF
Sbjct: 102 MWAIMTVVVVFESNVGATFCKCVNRVVATILAGSLGIAVHWVATQSGKA-EVFVIGCSVF 161

Query: 135 LIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGV 194
           L   AATY RF P  K  +DYG +IF+LTF+L+++  YRVD ++++A  R  TIAIG  +
Sbjct: 162 LFAFAATYSRFVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSI 221

Query: 195 CLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENK-ESCSRDQIYK 254
           C+++++   P W+G +LH      LE LA S++ CV +YF + E+  N+ E  + +   +
Sbjct: 222 CIIITVFFCPIWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRNEDENTNMKLQ 281

Query: 255 GYKAVLDSKSTDETLAL------------QASWEPRHSSHCYRIPWQQYVKLGGVLRHFG 314
           G+K VL+SK T+E + L             A WEP H S  +R PW+ YVK+G  +R   
Sbjct: 282 GFKCVLNSKGTEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCA 341

Query: 315 YTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSP-EILSD 374
           Y +  L  C+  E + P  V+  F + C +++   SK L ELA+ ++N R  S  + L  
Sbjct: 342 YCLENLSICINYETEAPDQVKNHFGEACMKLSSASSKILRELADMMKNTRKSSKMDFLVF 401

Query: 375 HLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVK 434
            ++ A+Q+L + +K+ P   + +N                 K EE   E+ +       K
Sbjct: 402 DMNSAVQELQETLKNVP---IETN-----------------KPEEVPSEEEN-------K 461

Query: 435 TDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILID 494
            DS    E RT                   TS+   E LP A   SLL+E  A++   ++
Sbjct: 462 VDS----EERT-------------------TSMSLHEVLPVATLVSLLIENAARIQTAVE 488

Query: 495 EVEELGRIACFKE---FKNGDD 500
            V+EL  +A F++    K GD+
Sbjct: 522 AVDELANLADFEQDSKKKTGDN 488

BLAST of Moc06g35740 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 8.4e-72
Identity = 145/347 (41.79%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 31  KTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAVMTVVVVLEFTAG 90
           K  +K   +DPRRI+HS+KVG++LTLVSLLY V PL+   G   +WA++TVVVV EFT G
Sbjct: 32  KNVQKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVG 91

Query: 91  ATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGGAATYMRFFPKIK 150
            TL KGLNRG  TL+AG+L      +A   G       +G  VF +G AAT+ RFFP+IK
Sbjct: 92  GTLSKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIK 151

Query: 151 KNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLMSLLIFPNWSGEE 210
           + YDYG +IF+LTF+ + IS YR D +L +A+ R  TI IG  +C+L+S+ I P W+GE+
Sbjct: 152 QRYDYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGED 211

Query: 211 LHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAVLDSKSTDETLAL 270
           LH      +  LAK +E    +YF   +I +   SC R+     YK++L SKST+++LA 
Sbjct: 212 LHKMIANNINKLAKYLEGFEGEYFQPEKISKETSSCVRE-----YKSILTSKSTEDSLAN 271

Query: 271 QASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTPRSVRVLFKDPCS 330
            A WEP H     R PW++Y+K+ G++R     +  L+G + +  + P+      ++P +
Sbjct: 272 LARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSNDKAPQEFESKIQEPIT 331

Query: 331 RVAREVSKALIELANSIRNRRHCSPEILS--DHLHEALQDLNKAIKS 376
            ++REV +AL  +A SI+  R+ S  + +  D+  +A+++L  A+KS
Sbjct: 332 TMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKS 373

BLAST of Moc06g35740 vs. ExPASy TrEMBL
Match: A0A6J1DWA3 (aluminum-activated malate transporter 12-like OS=Momordica charantia OX=3673 GN=LOC111023709 PE=3 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 4.2e-292
Identity = 524/526 (99.62%), Postives = 524/526 (99.62%), Query Frame = 0

Query: 1   MAESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLL 60
           MAESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLL
Sbjct: 1   MAESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLL 60

Query: 61  YLVEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRS 120
           YLVEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRS
Sbjct: 61  YLVEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRS 120

Query: 121 GRVFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKI 180
           GRVFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKI
Sbjct: 121 GRVFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKI 180

Query: 181 AHDRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID 240
           AHDRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID
Sbjct: 181 AHDRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID 240

Query: 241 ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHF 300
           ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHF
Sbjct: 241 ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHF 300

Query: 301 GYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSD 360
           GYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSD
Sbjct: 301 GYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSD 360

Query: 361 HLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVK 420
           HLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVK
Sbjct: 361 HLHEALQDLNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVK 420

Query: 421 TDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILID 480
           TDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILID
Sbjct: 421 TDSSALMEWRTKRREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILID 480

Query: 481 EVEELGRIACFKEFKNGDDEQHITVKCEKPKISVSQSQLSSVGHAE 527
           EVEELGRIACFKEFKNGDDEQHITVKCEKPKISVSQSQLSSV  AE
Sbjct: 481 EVEELGRIACFKEFKNGDDEQHITVKCEKPKISVSQSQLSSVSAAE 526

BLAST of Moc06g35740 vs. ExPASy TrEMBL
Match: A0A0A0LI60 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G903470 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.8e-263
Identity = 470/516 (91.09%), Postives = 497/516 (96.32%), Query Frame = 0

Query: 19  MDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAV 78
           M+KIKH P+LCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL++PLF+GIG NA+WAV
Sbjct: 1   MNKIKHFPILCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIQPLFQGIGNNALWAV 60

Query: 79  MTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGG 138
           MTVVVVLEFTAGATLCKGLNRGLGT+LAGSLAF IEGVANR+G+VFRACFIGAAVFLIG 
Sbjct: 61  MTVVVVLEFTAGATLCKGLNRGLGTVLAGSLAFFIEGVANRTGKVFRACFIGAAVFLIGS 120

Query: 139 AATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLM 198
            ATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDRFYTIAIGCGVCLLM
Sbjct: 121 VATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDRFYTIAIGCGVCLLM 180

Query: 199 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAV 258
           SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVN+YFFD EIDENKESCS DQIYKGYKAV
Sbjct: 181 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNEYFFDTEIDENKESCSGDQIYKGYKAV 240

Query: 259 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 318
           LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP
Sbjct: 241 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 300

Query: 319 RSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 378
           RSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR
Sbjct: 301 RSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 360

Query: 379 LFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSSALMEWRTKR----- 438
           LFLGSN+NQ+RNMLALAAA+AGQKQ  KEK+++SGVSLSSVKTDSSALMEW+TKR     
Sbjct: 361 LFLGSNKNQSRNMLALAAAEAGQKQ--KEKKRQSGVSLSSVKTDSSALMEWKTKRASEQS 420

Query: 439 REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILIDEVEELGRIACFKE 498
           REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDI+IDEVEELGRIACFKE
Sbjct: 421 REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDIVIDEVEELGRIACFKE 480

Query: 499 FKNGDD---EQHITVKCEKPKISVSQSQLSSVGHAE 527
           FK+GDD   E+HITVKCEKPKI+V+Q+QLSSV  AE
Sbjct: 481 FKHGDDDDKEEHITVKCEKPKINVTQNQLSSVSGAE 514

BLAST of Moc06g35740 vs. ExPASy TrEMBL
Match: A0A5D3E4X6 (Aluminum-activated malate transporter 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004270 PE=3 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 2.2e-261
Identity = 468/522 (89.66%), Postives = 496/522 (95.02%), Query Frame = 0

Query: 19  MDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAV 78
           M+KIKH PVLCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL++PLF+GIG+NA+WAV
Sbjct: 1   MNKIKHFPVLCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIQPLFEGIGSNALWAV 60

Query: 79  MTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGG 138
           MTVVVVLEFTAGATLCKGLNRGLGT+LAGSLAF IEGVANR+G+VFRACFIGAAVFLIG 
Sbjct: 61  MTVVVVLEFTAGATLCKGLNRGLGTVLAGSLAFFIEGVANRTGKVFRACFIGAAVFLIGS 120

Query: 139 AATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLM 198
            ATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDRFYTIAIGCGVCLLM
Sbjct: 121 VATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDRFYTIAIGCGVCLLM 180

Query: 199 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAV 258
           SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVN+YFFD EIDENKESCS DQIYKGYKAV
Sbjct: 181 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNEYFFDTEIDENKESCSGDQIYKGYKAV 240

Query: 259 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 318
           LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP
Sbjct: 241 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 300

Query: 319 RSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 378
           RSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR
Sbjct: 301 RSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 360

Query: 379 LFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSSALMEWRTKR----- 438
           LFLGSN+NQ+RNMLALAAA+AGQKQ EK+++  SGVSLSSVKTDSSALMEW+TKR     
Sbjct: 361 LFLGSNKNQSRNMLALAAAEAGQKQNEKKRQ--SGVSLSSVKTDSSALMEWKTKRASEQS 420

Query: 439 REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILIDEVEELGRIACFKE 498
           REAERKVLR QLSKIAITSLEFSEALPFAAFASLLVETVAKLDI+IDEVEELGRIACFKE
Sbjct: 421 REAERKVLRTQLSKIAITSLEFSEALPFAAFASLLVETVAKLDIVIDEVEELGRIACFKE 480

Query: 499 FKNGDD---------EQHITVKCEKPKISVSQSQLSSVGHAE 527
           FK+GDD         E+HITVKCEKPK++V+Q+QLSSV  AE
Sbjct: 481 FKHGDDNDNDNDNDEEEHITVKCEKPKVNVTQNQLSSVSGAE 520

BLAST of Moc06g35740 vs. ExPASy TrEMBL
Match: A0A1S3CRT0 (aluminum-activated malate transporter 12-like OS=Cucumis melo OX=3656 GN=LOC103504003 PE=3 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 2.2e-261
Identity = 468/522 (89.66%), Postives = 496/522 (95.02%), Query Frame = 0

Query: 19  MDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAV 78
           M+KIKH PVLCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL++PLF+GIG+NA+WAV
Sbjct: 1   MNKIKHFPVLCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIQPLFEGIGSNALWAV 60

Query: 79  MTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGG 138
           MTVVVVLEFTAGATLCKGLNRGLGT+LAGSLAF IEGVANR+G+VFRACFIGAAVFLIG 
Sbjct: 61  MTVVVVLEFTAGATLCKGLNRGLGTVLAGSLAFFIEGVANRTGKVFRACFIGAAVFLIGS 120

Query: 139 AATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLM 198
            ATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDRFYTIAIGCGVCLLM
Sbjct: 121 VATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDRFYTIAIGCGVCLLM 180

Query: 199 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAV 258
           SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVN+YFFD EIDENKESCS DQIYKGYKAV
Sbjct: 181 SLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNEYFFDTEIDENKESCSGDQIYKGYKAV 240

Query: 259 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 318
           LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP
Sbjct: 241 LDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTP 300

Query: 319 RSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 378
           RSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR
Sbjct: 301 RSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPR 360

Query: 379 LFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSSALMEWRTKR----- 438
           LFLGSN+NQ+RNMLALAAA+AGQKQ EK+++  SGVSLSSVKTDSSALMEW+TKR     
Sbjct: 361 LFLGSNKNQSRNMLALAAAEAGQKQNEKKRQ--SGVSLSSVKTDSSALMEWKTKRASEQS 420

Query: 439 REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILIDEVEELGRIACFKE 498
           REAERKVLR QLSKIAITSLEFSEALPFAAFASLLVETVAKLDI+IDEVEELGRIACFKE
Sbjct: 421 REAERKVLRTQLSKIAITSLEFSEALPFAAFASLLVETVAKLDIVIDEVEELGRIACFKE 480

Query: 499 FKNGDD---------EQHITVKCEKPKISVSQSQLSSVGHAE 527
           FK+GDD         E+HITVKCEKPK++V+Q+QLSSV  AE
Sbjct: 481 FKHGDDNDNDNDNDEEEHITVKCEKPKVNVTQNQLSSVSGAE 520

BLAST of Moc06g35740 vs. ExPASy TrEMBL
Match: A0A6J1GJX3 (aluminum-activated malate transporter 12-like OS=Cucurbita moschata OX=3662 GN=LOC111454996 PE=3 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 5.0e-261
Identity = 472/525 (89.90%), Postives = 494/525 (94.10%), Query Frame = 0

Query: 9   LKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFK 68
           ++VNK +Q++MDKI+H PVLCWKTSKKVGAEDPRRI+HSLKVGLSLTLVSLLYL+EPLFK
Sbjct: 1   MQVNKHIQVTMDKIQHFPVLCWKTSKKVGAEDPRRIIHSLKVGLSLTLVSLLYLIEPLFK 60

Query: 69  GIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACF 128
            +G+NA WAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAF IEGVANRSGRVFRACF
Sbjct: 61  RLGSNATWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFFIEGVANRSGRVFRACF 120

Query: 129 IGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTI 188
           IGAAVFLIG AATYMRFFPKIKKNYDYGVVIFLLTFNLIT+SSYRVDNVLKIAHDR YTI
Sbjct: 121 IGAAVFLIGAAATYMRFFPKIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDRLYTI 180

Query: 189 AIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSR 248
           AIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIE CVN+YFFD EIDENKESCS 
Sbjct: 181 AIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEGCVNEYFFDTEIDENKESCSG 240

Query: 249 DQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALH 308
           DQIYKGYKAVLDSKS DE+LALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALH
Sbjct: 241 DQIYKGYKAVLDSKSIDESLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALH 300

Query: 309 GCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQD 368
           GCLQTEIQTPRSVR+LFKDPC+RVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQD
Sbjct: 301 GCLQTEIQTPRSVRILFKDPCTRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQD 360

Query: 369 LNKAIKSQPRLFLGSNRNQARNMLALAAAQAGQKQEEKEKEKRSGVSLSSVKTDSSALME 428
           LNKAIKSQPRLFLGSNRN+A NMLALAAA+AGQK+       RSG SLSSVKTDSSALME
Sbjct: 361 LNKAIKSQPRLFLGSNRNKASNMLALAAAEAGQKR------CRSGGSLSSVKTDSSALME 420

Query: 429 WRTKR-----REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDILIDEVE 488
           W+TKR     REAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLD +IDEVE
Sbjct: 421 WKTKRASEQSREAERKVLRPQLSKIAITSLEFSEALPFAAFASLLVETVAKLDNVIDEVE 480

Query: 489 ELGRIACFKEFKNGDD--EQHITVKCEKPKISVSQSQLSSVGHAE 527
           ELGRIACFKEF NGDD  EQHITVKCEKP I+VSQ+QLSSVG AE
Sbjct: 481 ELGRIACFKEFINGDDHEEQHITVKCEKPNINVSQNQLSSVGGAE 519

BLAST of Moc06g35740 vs. TAIR 10
Match: AT4G17970.1 (aluminum-activated, malate transporter 12 )

HSP 1 Score: 646.4 bits (1666), Expect = 2.6e-185
Identity = 351/529 (66.35%), Postives = 415/529 (78.45%), Query Frame = 0

Query: 20  DKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWAVM 79
           +KIK +P   W     VG EDPRR++H+LKVGLSLTLVSLLYL+EPLFKGIG+NAIWAVM
Sbjct: 28  EKIKKIPKRLW----NVGKEDPRRVIHALKVGLSLTLVSLLYLMEPLFKGIGSNAIWAVM 87

Query: 80  TVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIGGA 139
           TVVVVLEF+AGATLCKGLNRGLGTL+AGSLAF IE VAN SG+V RA FIG AVF+IG A
Sbjct: 88  TVVVVLEFSAGATLCKGLNRGLGTLIAGSLAFFIEFVANDSGKVLRAIFIGTAVFIIGAA 147

Query: 140 ATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLLMS 199
           ATY+RF P IKKNYDYGVVIFLLTFNLIT+SSYRVD+V+ IAHDRFYTIA+GCG+CL MS
Sbjct: 148 ATYIRFIPYIKKNYDYGVVIFLLTFNLITVSSYRVDSVINIAHDRFYTIAVGCGICLFMS 207

Query: 200 LLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKAVL 259
           LL+FP WSGE+LH +TV KL+GL++SIEACV++YF   E  E +++ S+D+IY+GY+AVL
Sbjct: 208 LLVFPIWSGEDLHKTTVGKLQGLSRSIEACVDEYF---EEKEKEKTDSKDRIYEGYQAVL 267

Query: 260 DSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQTPR 319
           DSKSTDETLAL A+WEPRH+  C+R P QQYVK+G VLR FGYTVVALHGCLQTEIQTPR
Sbjct: 268 DSKSTDETLALYANWEPRHTLRCHRFPCQQYVKVGAVLRQFGYTVVALHGCLQTEIQTPR 327

Query: 320 SVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQPRL 379
           SVR LFKDPC R+A EV KAL ELA+SI N RHCSPEILSDHLH ALQDLN AIKSQP+L
Sbjct: 328 SVRALFKDPCVRLAGEVCKALTELADSISNHRHCSPEILSDHLHVALQDLNSAIKSQPKL 387

Query: 380 FLGSNRNQARNM--------------------------LALAAAQAGQKQ--EEKEKEKR 439
           FLGSN ++  N                           ++L   + G ++  E   ++ +
Sbjct: 388 FLGSNLHRHNNKHQNGSISNNKHHQRNSSNSGKDLNGDVSLQNTETGTRKITETGSRQGQ 447

Query: 440 SG-VSLSSVKTDSSALMEWRTKRREA--------ERKVLRPQLSKIAI-TSLEFSEALPF 499
           +G VSLSS +TD+SALME+R   + +        ER++LRPQLSKIA+ TSLEFSEALPF
Sbjct: 448 NGAVSLSSFRTDTSALMEYRRSFKNSNSEMSAAGERRMLRPQLSKIAVMTSLEFSEALPF 507

Query: 500 AAFASLLVETVAKLDILIDEVEELGRIACFKEFKNGDDEQHITVKCEKP 511
           AAFASLLVE VA+LD +I+EVEELGRIA FKE+ N  D+    V+CE P
Sbjct: 508 AAFASLLVEMVARLDNVIEEVEELGRIASFKEYDNKRDQTADDVRCENP 549

BLAST of Moc06g35740 vs. TAIR 10
Match: AT5G46610.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 617.1 bits (1590), Expect = 1.7e-176
Identity = 332/515 (64.47%), Postives = 392/515 (76.12%), Query Frame = 0

Query: 21  KIKHLPVLCWKTSK---KVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWA 80
           K+  LP    K  K   KVG +DPRR+ H+LKVG+SLTLVSLLYL+EPLFKGIG +AIWA
Sbjct: 24  KVLELPTKIKKILKNIWKVGKDDPRRVKHALKVGVSLTLVSLLYLMEPLFKGIGNSAIWA 83

Query: 81  VMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIG 140
           VMTVVVVLEF+AGATLCKGLNRGLGTL+AGSLAF IE VAN SG++FRA FIGAAVF+IG
Sbjct: 84  VMTVVVVLEFSAGATLCKGLNRGLGTLIAGSLAFFIEFVANDSGKIFRAIFIGAAVFIIG 143

Query: 141 GAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLL 200
              TY+RF P IKKNYDYG++IFLLTFNLIT+SSYRVD V+KIAH+RFYTIA+G G+CLL
Sbjct: 144 ALITYLRFIPYIKKNYDYGMLIFLLTFNLITVSSYRVDTVIKIAHERFYTIAMGVGICLL 203

Query: 201 MSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKA 260
           MSLL+FP WSGE+LH STV KL+GL+ SIEACVN+YF + E DE     S D IY GYK 
Sbjct: 204 MSLLVFPIWSGEDLHKSTVAKLQGLSYSIEACVNEYFEEEEKDEETSDLSEDTIYNGYKT 263

Query: 261 VLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQT 320
           VLDSKS DE LA+ ASWEPRH+ HC+R PW+ YVK+G VLR FGYTVVALHGCL+TEIQT
Sbjct: 264 VLDSKSADEALAMYASWEPRHTRHCHRFPWKHYVKVGSVLRQFGYTVVALHGCLKTEIQT 323

Query: 321 PRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQP 380
           PR +R LFKDPC R+A E+ K L ELA SIRNRRHCSPEILSD L  ALQDLN AIKSQP
Sbjct: 324 PRPLRGLFKDPCVRLAGEICKVLSELAASIRNRRHCSPEILSDSLQVALQDLNTAIKSQP 383

Query: 381 RLFLGSNR-------NQARNMLALAAAQ--------------AGQKQEEKEKEKRSGVSL 440
           +LFLGS++       N  R+   +A +Q               G  + E+       VS 
Sbjct: 384 KLFLGSSQNGNVSQGNSGRHNPNVAVSQHINKDTNEAASYQNTGTPRGERMSRFGPNVSF 443

Query: 441 SSVKTDSSALMEWRTKRREAERKVLRPQLSKIAI-TSLEFSEALPFAAFASLLVETVAKL 500
           S ++ D+   +E R+     ERK+LR QLS+I + TSLEFSEALPFAAFASLLVE VA+L
Sbjct: 444 SRLRADT---LERRSAAATNERKILRQQLSRIVVLTSLEFSEALPFAAFASLLVEMVARL 503

Query: 501 DILIDEVEELGRIACFKEFKNGDDEQHITVKCEKP 511
           D +I+EVEELG IACFK++ N  D++ + V+ EKP
Sbjct: 504 DNVIEEVEELGTIACFKDYDNNVDQKDVEVRVEKP 535

BLAST of Moc06g35740 vs. TAIR 10
Match: AT5G46610.2 (Aluminium activated malate transporter family protein )

HSP 1 Score: 562.0 bits (1447), Expect = 6.4e-160
Identity = 310/515 (60.19%), Postives = 369/515 (71.65%), Query Frame = 0

Query: 21  KIKHLPVLCWKTSK---KVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWA 80
           K+  LP    K  K   KVG +DPRR+ H+LKVG+SLTLVSLLYL+EPLFKGIG +AIWA
Sbjct: 24  KVLELPTKIKKILKNIWKVGKDDPRRVKHALKVGVSLTLVSLLYLMEPLFKGIGNSAIWA 83

Query: 81  VMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIG 140
           VMTVVVVLEF+A                        E VAN SG++FRA FIGAAVF+IG
Sbjct: 84  VMTVVVVLEFSA------------------------EFVANDSGKIFRAIFIGAAVFIIG 143

Query: 141 GAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLL 200
              TY+RF P IKKNYDYG++IFLLTFNLIT+SSYRVD V+KIAH+RFYTIA+G G+CLL
Sbjct: 144 ALITYLRFIPYIKKNYDYGMLIFLLTFNLITVSSYRVDTVIKIAHERFYTIAMGVGICLL 203

Query: 201 MSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKA 260
           MSLL+FP WSGE+LH STV KL+GL+ SIEACVN+YF + E DE     S D IY GYK 
Sbjct: 204 MSLLVFPIWSGEDLHKSTVAKLQGLSYSIEACVNEYFEEEEKDEETSDLSEDTIYNGYKT 263

Query: 261 VLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQT 320
           VLDSKS DE LA+ ASWEPRH+ HC+R PW+ YVK+G VLR FGYTVVALHGCL+TEIQT
Sbjct: 264 VLDSKSADEALAMYASWEPRHTRHCHRFPWKHYVKVGSVLRQFGYTVVALHGCLKTEIQT 323

Query: 321 PRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQP 380
           PR +R LFKDPC R+A E+ K L ELA SIRNRRHCSPEILSD L  ALQDLN AIKSQP
Sbjct: 324 PRPLRGLFKDPCVRLAGEICKVLSELAASIRNRRHCSPEILSDSLQVALQDLNTAIKSQP 383

Query: 381 RLFLGSNR-------NQARNMLALAAAQ--------------AGQKQEEKEKEKRSGVSL 440
           +LFLGS++       N  R+   +A +Q               G  + E+       VS 
Sbjct: 384 KLFLGSSQNGNVSQGNSGRHNPNVAVSQHINKDTNEAASYQNTGTPRGERMSRFGPNVSF 443

Query: 441 SSVKTDSSALMEWRTKRREAERKVLRPQLSKIAI-TSLEFSEALPFAAFASLLVETVAKL 500
           S ++ D+   +E R+     ERK+LR QLS+I + TSLEFSEALPFAAFASLLVE VA+L
Sbjct: 444 SRLRADT---LERRSAAATNERKILRQQLSRIVVLTSLEFSEALPFAAFASLLVEMVARL 503

Query: 501 DILIDEVEELGRIACFKEFKNGDDEQHITVKCEKP 511
           D +I+EVEELG IACFK++ N  D++ + V+ EKP
Sbjct: 504 DNVIEEVEELGTIACFKDYDNNVDQKDVEVRVEKP 511

BLAST of Moc06g35740 vs. TAIR 10
Match: AT5G46600.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 542.7 bits (1397), Expect = 4.0e-154
Identity = 302/518 (58.30%), Postives = 363/518 (70.08%), Query Frame = 0

Query: 3   ESDHQKLKVNKSLQMSMDKIKHLPVLCWKTSKKVGAEDPRRIVHSLKVGLSLTLVSLLYL 62
           E   +K K   +L   M KI        +    VG EDPRR++H+LKVG++LTLVSLLYL
Sbjct: 17  EDSRKKRKKGLNLPKKMKKI-------LRNLWNVGKEDPRRVIHALKVGVALTLVSLLYL 76

Query: 63  VEPLFKGIGTNAIWAVMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGR 122
           +EP F+G+G NA+WAVMTVVVVLEF+AGATL KGLNRGLGTL+AGSLAF IE VA  SG+
Sbjct: 77  MEPFFEGVGKNALWAVMTVVVVLEFSAGATLRKGLNRGLGTLIAGSLAFFIEWVAIHSGK 136

Query: 123 VFRACFIGAAVFLIGGAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAH 182
           +    FIG +VF IG   TYMRF P IKKNYDYG+++FLLTFNLIT+SSYRVD V+KIAH
Sbjct: 137 ILGGIFIGTSVFTIGSMITYMRFIPYIKKNYDYGMLVFLLTFNLITVSSYRVDTVIKIAH 196

Query: 183 DRFYTIAIGCGVCLLMSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEID-- 242
           +R YTI +G G+CL MSLL FP WSG++LH ST+ KL+GL++ IEACV++YF +   D  
Sbjct: 197 ERLYTIGMGIGICLFMSLLFFPIWSGDDLHKSTITKLQGLSRCIEACVSEYFEEKLKDNE 256

Query: 243 -ENKESCSRDQIYKGYKAVLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRH 302
             + ES   D IY GY  VLDSKS DE LA+ A WEPRH+  C + P QQY+K+G VLR 
Sbjct: 257 TSDSESDDEDLIYNGYNTVLDSKSADEALAMYAKWEPRHTRRCNKFPSQQYIKVGSVLRK 316

Query: 303 FGYTVVALHGCLQTEIQTPRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILS 362
           FGYTVVALHGCLQTEIQTPRS+RVLFKDPC R+A E+ K L EL+ SI+NRRHCS EILS
Sbjct: 317 FGYTVVALHGCLQTEIQTPRSIRVLFKDPCVRLAGEICKVLSELSESIQNRRHCSSEILS 376

Query: 363 DHLHEALQDLNKAIKSQPRLFLGSN--RNQARNMLALAAAQAGQKQEE------------ 422
           D L  AL+DLN  IKSQP+LFLGSN   N     L    +   +                
Sbjct: 377 DSLEAALKDLNSTIKSQPKLFLGSNLHSNITNKHLNGHVSYYNETNSNGTVSYHNDNNTN 436

Query: 423 --------KEKEKRSGVSLSSVKTDSSALMEWRTKRREAERKVLRPQLSKIAI-TSLEFS 482
                   +E +  S + L+SV    S+L   +      E++ LR QLSKIA+  SLEFS
Sbjct: 437 GCVLGETIEENDTVSPLPLNSV-VSLSSLRSVKKSAATGEKRRLRKQLSKIAVMKSLEFS 496

Query: 483 EALPFAAFASLLVETVAKLDILIDEVEELGRIACFKEF 495
           EALPFAAFASLLVE VA+LD +IDEVEELG IACFKE+
Sbjct: 497 EALPFAAFASLLVEMVARLDTVIDEVEELGTIACFKEY 526

BLAST of Moc06g35740 vs. TAIR 10
Match: AT5G46610.3 (Aluminium activated malate transporter family protein )

HSP 1 Score: 523.9 bits (1348), Expect = 1.9e-148
Identity = 294/515 (57.09%), Postives = 351/515 (68.16%), Query Frame = 0

Query: 21  KIKHLPVLCWKTSK---KVGAEDPRRIVHSLKVGLSLTLVSLLYLVEPLFKGIGTNAIWA 80
           K+  LP    K  K   KVG +DPRR+ H+LKVG+SLTLVSLLYL+EPLFKGIG +AIWA
Sbjct: 24  KVLELPTKIKKILKNIWKVGKDDPRRVKHALKVGVSLTLVSLLYLMEPLFKGIGNSAIWA 83

Query: 81  VMTVVVVLEFTAGATLCKGLNRGLGTLLAGSLAFLIEGVANRSGRVFRACFIGAAVFLIG 140
           VMTVVVVLEF+AGA +                                            
Sbjct: 84  VMTVVVVLEFSAGALI-------------------------------------------- 143

Query: 141 GAATYMRFFPKIKKNYDYGVVIFLLTFNLITISSYRVDNVLKIAHDRFYTIAIGCGVCLL 200
              TY+RF P IKKNYDYG++IFLLTFNLIT+SSYRVD V+KIAH+RFYTIA+G G+CLL
Sbjct: 144 ---TYLRFIPYIKKNYDYGMLIFLLTFNLITVSSYRVDTVIKIAHERFYTIAMGVGICLL 203

Query: 201 MSLLIFPNWSGEELHNSTVLKLEGLAKSIEACVNDYFFDAEIDENKESCSRDQIYKGYKA 260
           MSLL+FP WSGE+LH STV KL+GL+ SIEACVN+YF + E DE     S D IY GYK 
Sbjct: 204 MSLLVFPIWSGEDLHKSTVAKLQGLSYSIEACVNEYFEEEEKDEETSDLSEDTIYNGYKT 263

Query: 261 VLDSKSTDETLALQASWEPRHSSHCYRIPWQQYVKLGGVLRHFGYTVVALHGCLQTEIQT 320
           VLDSKS DE LA+ ASWEPRH+ HC+R PW+ YVK+G VLR FGYTVVALHGCL+TEIQT
Sbjct: 264 VLDSKSADEALAMYASWEPRHTRHCHRFPWKHYVKVGSVLRQFGYTVVALHGCLKTEIQT 323

Query: 321 PRSVRVLFKDPCSRVAREVSKALIELANSIRNRRHCSPEILSDHLHEALQDLNKAIKSQP 380
           PR +R LFKDPC R+A E+ K L ELA SIRNRRHCSPEILSD L  ALQDLN AIKSQP
Sbjct: 324 PRPLRGLFKDPCVRLAGEICKVLSELAASIRNRRHCSPEILSDSLQVALQDLNTAIKSQP 383

Query: 381 RLFLGSNR-------NQARNMLALAAAQ--------------AGQKQEEKEKEKRSGVSL 440
           +LFLGS++       N  R+   +A +Q               G  + E+       VS 
Sbjct: 384 KLFLGSSQNGNVSQGNSGRHNPNVAVSQHINKDTNEAASYQNTGTPRGERMSRFGPNVSF 443

Query: 441 SSVKTDSSALMEWRTKRREAERKVLRPQLSKIAI-TSLEFSEALPFAAFASLLVETVAKL 500
           S ++ D+   +E R+     ERK+LR QLS+I + TSLEFSEALPFAAFASLLVE VA+L
Sbjct: 444 SRLRADT---LERRSAAATNERKILRQQLSRIVVLTSLEFSEALPFAAFASLLVEMVARL 488

Query: 501 DILIDEVEELGRIACFKEFKNGDDEQHITVKCEKP 511
           D +I+EVEELG IACFK++ N  D++ + V+ EKP
Sbjct: 504 DNVIEEVEELGTIACFKDYDNNVDQKDVEVRVEKP 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156876.18.6e-29299.62aluminum-activated malate transporter 12-like [Momordica charantia][more]
XP_038888238.13.3e-26790.72aluminum-activated malate transporter 12-like [Benincasa hispida][more]
XP_004148510.23.7e-26689.62aluminum-activated malate transporter 12 [Cucumis sativus] >KAE8651447.1 hypothe... [more]
XP_008466647.24.6e-26189.66PREDICTED: aluminum-activated malate transporter 12-like [Cucumis melo] >KAA0038... [more]
XP_022952267.11.0e-26089.90aluminum-activated malate transporter 12-like [Cucurbita moschata] >KAG6571842.1... [more]
Match NameE-valueIdentityDescription
O496963.6e-18466.35Aluminum-activated malate transporter 12 OS=Arabidopsis thaliana OX=3702 GN=ALMT... [more]
Q9LS222.4e-17564.47Aluminum-activated malate transporter 14 OS=Arabidopsis thaliana OX=3702 GN=ALMT... [more]
Q9LS235.7e-15358.30Aluminum-activated malate transporter 13 OS=Arabidopsis thaliana OX=3702 GN=ALMT... [more]
O230862.1e-7535.26Aluminum-activated malate transporter 10 OS=Arabidopsis thaliana OX=3702 GN=ALMT... [more]
Q9SRM98.4e-7241.79Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Match NameE-valueIdentityDescription
A0A6J1DWA34.2e-29299.62aluminum-activated malate transporter 12-like OS=Momordica charantia OX=3673 GN=... [more]
A0A0A0LI601.8e-26391.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G903470 PE=3 SV=1[more]
A0A5D3E4X62.2e-26189.66Aluminum-activated malate transporter 12-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3CRT02.2e-26189.66aluminum-activated malate transporter 12-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1GJX35.0e-26189.90aluminum-activated malate transporter 12-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT4G17970.12.6e-18566.35aluminum-activated, malate transporter 12 [more]
AT5G46610.11.7e-17664.47Aluminium activated malate transporter family protein [more]
AT5G46610.26.4e-16060.19Aluminium activated malate transporter family protein [more]
AT5G46600.14.0e-15458.30Aluminium activated malate transporter family protein [more]
AT5G46610.31.9e-14857.09Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 35..486
e-value: 4.9E-174
score: 579.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 397..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 401..415
NoneNo IPR availablePANTHERPTHR31086:SF2ALUMINUM-ACTIVATED MALATE TRANSPORTER 12-RELATEDcoord: 4..517
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 4..517

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g35740.1Moc06g35740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane