Moc06g35670 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g35670
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	beta-amyrin 11-oxidase-like
Location	chr6: 27387207 .. 27389894 (+)
RNA-Seq Expression	Moc06g35670
Synteny	Moc06g35670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTAGAAGTTTTTTGGTTTACAATGCCCTCCCTCCAGGGGATATGGGATGGCCTCTTGTTGGTTCTTCTTTATCGTTCTACCAAGCATTTAAAATCGGTGGCGACCCTAACTCTGTTATGGATAATCTCATTTCTAGGTATCGTCTCTCATTGACTTCTTCCTTTTTTTATGGATAAGATCGTAAATGAAATGACTAGGTATGTACGGTAAATCTACCCATTAATTTTGAGTTTGATATTCCTTTCAATTTGTGGTTTTAATTTAAATATTTGTGTGGCTAGTTACGTTATATTAAAAGTGATTAATTTTTTTTTTTTTTTTTTTTTGGTGAGAATTACGCTTTCATCAAGGAAACTAAACCAATTGGGTAACTTTTGAGGGCTGAATCGGCCACAGCTTTACAAAGCCAAGGGGTAAAAAAGTCAATCCCCAAAGTACAGATTCGATCAGGCTTTGCCAAACAATCTACAGTGGAATTGAACAACATGGGAAAGCGATTTTAATTATAAGTTTGTTTGTTGCTTTAGCCGTGATTCTGTAGATGTCAAATTACTAATAATGATTTTAAAATGATGTTCCATTAATCAGTTTAGAATCACTTCTAAAAGGTAGGGCTACAAAAGAAATTAAAGTATCATAGAAAAAACAAAGTTAATATTGTTAAATAAAATTTCATTGCCAAAAACGAACATAACTCAGCAGTGATTGACATATACTTCATACCGAGAGATTCTGAGTTCGATCTCACACCCTCAAATGTTGTATTTAAAAAAAAAATTGAACTTTCCATTTTGTGAGGAAATAATGAAAGTTACGGCTAGATTTTCAATTTAGACAAAATCAACATTAGAATTGTTCTAAATTTGAAAATTTTGACAGAATTATTTAATAGTAGATTTTGTGTATAATCTTGAATAATCTCGAATGTTATCCGTTCCGGTACGTAGACCAAAAGAGCTTGAAGTTGGAATTCAATAGTCTACAAGCTTGCCTTGAAACGACTACAAGTGCGTGCATTTCCCTATCAATGTGAAGGGGATACAGGTCCGAGTTATTTGTTCCTAAATTTTAATTACTATTACAATTTACCACTTTCATTTTTTATTAATTTTTTTCCTAATACGTAAAAAAAACTGTAAAATCTTACCACTTTAATGAAAATCAAGTTTTACTCAAAATTAAATTTAAATGTACACAATCGACTATTAAATAACTCCCTAAAATTTTATAAATTTTAACGTATATCAATACATTAAAAGCCACTCAACTATTTTTCAAAAGAAAATTTATTAGAAGGTAAAAATTTCAATACTTTTTAAATTAAAGATGTAATTTGCAACAACTTAGAGTCTTTCGACCCTTTTTCAATGATATAAACTATTACAATAGAAATTGATATTTTCAAAAAAAAAAAACAAAAAAGTGGTACAGCTTATCTTAGGTTTAGTGCTATTAGTTTGCTTCTCATTAATTCCATTCAGAAAATATTATTTCTTGACTTTGTTTTTCTTTGATTTATATAATATCTTGGAAATGAATACATAACATTGTGCCCCCTTTTGACATAAATTCTTAGATCCGCCTTGAAATAACTTGTTAATAATATAAACAATTGATTAAATATACTGCAGCCATCTTAGGTTTTTTTTGTTTATACAACATTTGAATTATTTGTAATGTTAATTTTTTTTTCCCGAATTTAAAGTCCTTCTTCATGAACTTAATAGCTACTTGTTGGAGTCTCACAACGAAACAGAGCAATTAGAATTATGAACAATTAATTAAATTTGTAATTTGTAAGCTTTTTAGTTTAGCACCTAATTACTAATATTTATGGTTACTAAATTACTAAATGTACCTTAGCCTATTAAATTTAAGTTTTTTTTTTTCAAGTATAGGTGTCAATTAATTCTAAGCTAGGTTCATGTTAACTTTAATATACATGTTTAAATACATGTTTGTTCCTAATGACAGATTTGGAAGAGTTAGGATGTACAAAAGTCACCTGTACGGTTTTCCAGTGATCATCGTGACAGATCCAGAAGTATGCCGACGCATATACTTAGACGACGAACATTTCAAGTTGAACTATCCAAAGTCAGTGAAACTATTACAAGCAGATGGTTCACTATTGAAGGTCGACTACAAAATTGTGCACCGCTTGATGGCAGCTCCGATAAACGGCGTCGAGGCGTTGTCTCGGTATGTCGAGTTCATCGAAGATGCGGTGGTGGAGGGTTTGGAGGAATGGGGCAGCATGAAAGGCCCAGTGCCGTTGCTTCATGAATTCAAACGCCTCACCTTCAAGATCATTATAGGGGTTTTCTTGGGGTCTGTTTTCACTGACTCTGAAGTAGCAGAACTTGAGAGTTTGTACGCAGAGATCGGACCAGCAATCATGTCCATTTTCCCTTATAACTTGCCAGGATTCAGTTACCGTCGAGCACTCAAAGTACATCATCCATTAACTCTGCCCCTCATAAATAATCTACATATTATATATAGTTAATAGTAATTACTTATCTGCTATCAATATTCATGAGGTTTCATCTCGAAATCTAGAAGTGAATGATCGTGGGTATATAAGAGAGGAGAAGCAAAGCCGTGAAGGATATATGCTCAAAGCAGATCATATCATATCATTGTGGAGATACTTTGGATATCTGGGGTACCGTTGTCCTTAA

mRNA sequence

ATGTCTAGAAGTTTTTTGGTTTACAATGCCCTCCCTCCAGGGGATATGGGATGGCCTCTTGTTGGTTCTTCTTTATCGTTCTACCAAGCATTTAAAATCGGTGGCGACCCTAACTCTGTTATGGATAATCTCATTTCTAGATTTGGAAGAGTTAGGATGTACAAAAGTCACCTGTACGGTTTTCCAGTGATCATCGTGACAGATCCAGAAGTATGCCGACGCATATACTTAGACGACGAACATTTCAAGTTGAACTATCCAAAGTCAGTGAAACTATTACAAGCAGATGGTTCACTATTGAAGGTCGACTACAAAATTGTGCACCGCTTGATGGCAGCTCCGATAAACGGCGTCGAGGCGTTGTCTCGGTATGTCGAGTTCATCGAAGATGCGGTGGTGGAGGGTTTGGAGGAATGGGGCAGCATGAAAGGCCCAGTGCCGTTGCTTCATGAATTCAAACGCCTCACCTTCAAGATCATTATAGGGGTTTTCTTGGGGTCTGTTTTCACTGACTCTGAAGTAGCAGAACTTGAGAGTTTGTACGCAGAGATCGGACCAGCAATCATGTCCATTTTCCCTTATAACTTGCCAGGATTCAGTTACCGTCGAGCACTCAAAGTTTCATCTCGAAATCTAGAAGTGAATGATCGTGGGTATATAAGAGAGGAGAAGCAAAGCCGTGAAGGATATATGCTCAAAGCAGATCATATCATATCATTGTGGAGATACTTTGGATATCTGGGGTACCGTTGTCCTTAA

Coding sequence (CDS)

ATGTCTAGAAGTTTTTTGGTTTACAATGCCCTCCCTCCAGGGGATATGGGATGGCCTCTTGTTGGTTCTTCTTTATCGTTCTACCAAGCATTTAAAATCGGTGGCGACCCTAACTCTGTTATGGATAATCTCATTTCTAGATTTGGAAGAGTTAGGATGTACAAAAGTCACCTGTACGGTTTTCCAGTGATCATCGTGACAGATCCAGAAGTATGCCGACGCATATACTTAGACGACGAACATTTCAAGTTGAACTATCCAAAGTCAGTGAAACTATTACAAGCAGATGGTTCACTATTGAAGGTCGACTACAAAATTGTGCACCGCTTGATGGCAGCTCCGATAAACGGCGTCGAGGCGTTGTCTCGGTATGTCGAGTTCATCGAAGATGCGGTGGTGGAGGGTTTGGAGGAATGGGGCAGCATGAAAGGCCCAGTGCCGTTGCTTCATGAATTCAAACGCCTCACCTTCAAGATCATTATAGGGGTTTTCTTGGGGTCTGTTTTCACTGACTCTGAAGTAGCAGAACTTGAGAGTTTGTACGCAGAGATCGGACCAGCAATCATGTCCATTTTCCCTTATAACTTGCCAGGATTCAGTTACCGTCGAGCACTCAAAGTTTCATCTCGAAATCTAGAAGTGAATGATCGTGGGTATATAAGAGAGGAGAAGCAAAGCCGTGAAGGATATATGCTCAAAGCAGATCATATCATATCATTGTGGAGATACTTTGGATATCTGGGGTACCGTTGTCCTTAA

Protein sequence

MSRSFLVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKVSSRNLEVNDRGYIREEKQSREGYMLKADHIISLWRYFGYLGYRCP

Homology

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BLAST of Moc06g35670 vs. NCBI nr
Match: XP_022158172.1 (beta-amyrin 11-oxidase-like [Momordica charantia])

HSP 1 Score: 422.2 bits (1084), Expect = 3.2e-114
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MSRSFLVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYG 60
           MSRSFLVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYG
Sbjct: 1   MSRSFLVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYG 60

Query: 61  FPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEA 120
           FPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEA
Sbjct: 61  FPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEA 120

Query: 121 LSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESL 180
           LSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLG VFTDSEVAELESL
Sbjct: 121 LSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGXVFTDSEVAELESL 180

Query: 181 YAEIGPAIMSIFPYNLPGFSYRRALKV 208
           YAEIGPAIMSIFPYNLPGFSYRRALKV
Sbjct: 181 YAEIGPAIMSIFPYNLPGFSYRRALKV 207

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BLAST of Moc06g35670 vs. NCBI nr
Match: XP_022157153.1 (beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 408.3 bits (1048), Expect = 4.8e-110
Identity = 203/222 (91.44%), Postives = 209/222 (94.14%), Query Frame = 0


Query: 7   VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIV 66
           VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPN+VMDNLISRFGRVRMYKSHLYGFPVIIV
Sbjct: 41  VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIV 100

Query: 67  TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 126
           TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE
Sbjct: 101 TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 160

Query: 127 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGP 186
           FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLG VFTDSEVAELESLYAEIGP
Sbjct: 161 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGP 220

Query: 187 AIMSIFPYNLPGFSYRRALKVSSRNLEVNDRGYIREEKQSRE 229
           AIMSIFPYNLPGFSYRRALK   + +E   R  I+  K++ E
Sbjct: 221 AIMSIFPYNLPGFSYRRALKARIK-VENIVRPVIQNRKKALE 261

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BLAST of Moc06g35670 vs. NCBI nr
Match: XP_022157134.1 (beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 349.4 bits (895), Expect = 2.7e-92
Identity = 163/204 (79.90%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 7   VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIV 66
           +YNALPPG MGWPL+GSSLSFY+AFKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIV
Sbjct: 41  IYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIV 100

Query: 67  TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 126
           TDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ 
Sbjct: 101 TDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMG 160

Query: 127 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGP 186
           FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLG  FTDSE+A+LE+LYAEIG 
Sbjct: 161 FIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGL 220

Query: 187 AIMSIFPYNLPGFSYRRALKVSSR 211
           AI+SIFPY+LPGFSYRRALK  S+
Sbjct: 221 AILSIFPYDLPGFSYRRALKARSK 244

[Close]

BLAST of Moc06g35670 vs. NCBI nr
Match: BBI21930.1 (cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 349.4 bits (895), Expect = 2.7e-92
Identity = 163/204 (79.90%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 7   VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIV 66
           +YNALPPG MGWPL+GSSLSFY+AFKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIV
Sbjct: 41  IYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIV 100

Query: 67  TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 126
           TDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ 
Sbjct: 101 TDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMG 160

Query: 127 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGP 186
           FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLG  FTDSE+A+LE+LYAEIG 
Sbjct: 161 FIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGL 220

Query: 187 AIMSIFPYNLPGFSYRRALKVSSR 211
           AI+SIFPY+LPGFSYRRALK  S+
Sbjct: 221 AILSIFPYDLPGFSYRRALKARSK 244

[Close]

BLAST of Moc06g35670 vs. NCBI nr
Match: XP_022157141.1 (beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 332.0 bits (850), Expect = 4.4e-87
Identity = 156/195 (80.00%), Postives = 180/195 (92.31%), Query Frame = 0


Query: 16  MGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRI 75
           MGWPL+GSSLSFY+AFKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 76  YLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEG 135
           YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+G
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 136 LEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPAIMSIFPYN 195
           LEEWGS+KGPV LL EFKRLTFK+I GVFLG  FTDSE+A+LE+LYAEIG AI+SIFPY+
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 196 LPGFSYRRALKVSSR 211
           LPGFSYRRALK  S+
Sbjct: 181 LPGFSYRRALKARSK 195

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BLAST of Moc06g35670 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.4e-43
Identity = 88/203 (43.35%), Postives = 137/203 (67.49%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPGDMGWPL+G  LSF + F   G P+S ++NL+ ++GR  +YK+HL+G P IIV +P+
Sbjct: 43  LPPGDMGWPLIGDLLSFIKDFS-SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQ 102

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVEF 130
           +CRR+  DD +FKL YPKS+K L     ++ V   ++++  RL+ +PI G +AL+ Y+E 
Sbjct: 103 MCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLER 162

Query: 131 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPA 190
           +E+ V+  LEE  SMK PV LL E K+++FK I+ VF+GS   D  + ++ S + ++   
Sbjct: 163 LEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVHVFMGSSNQDI-IKKIGSSFTDLYNG 222

Query: 191 IMSIFPYNLPGFSYRRALKVSSR 211
           + SI P N+PGF++ +AL+   +
Sbjct: 223 MFSI-PINVPGFTFHKALEARKK 242

[Close]

BLAST of Moc06g35670 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 1.0e-41
Identity = 84/203 (41.38%), Postives = 130/203 (64.04%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPGD+GWP++G+  SF +AFK   DP S + + I+R+GR  +YK+H++G+P ++VT PE
Sbjct: 44  LPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPE 103

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVEF 130
            CRR+  DD+ F + +PKS   L    S + +   ++K + RL +AP+NG EALS Y++F
Sbjct: 104 TCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQF 163

Query: 131 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPA 190
           IE+ V   LE+W  M G +  L   ++LTFK+I+ +FL S  ++  +  LE  Y  +   
Sbjct: 164 IEETVNTDLEKWSKM-GEIEFLSHLRKLTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYG 223

Query: 191 IMSIFPYNLPGFSYRRALKVSSR 211
           + ++   NLPGF+Y RALK   +
Sbjct: 224 VRAM-GINLPGFAYHRALKARKK 242

[Close]

BLAST of Moc06g35670 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 8.7e-38
Identity = 84/224 (37.50%), Postives = 130/224 (58.04%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPGDMGWPLVG   +F +AFK  G P+S +D+   RFGR  +Y++ ++  P I+ T PE
Sbjct: 45  LPPGDMGWPLVGGMWAFLRAFK-SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPE 104

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDY---KIVHRLMAAPINGVEALSRYVEF 130
            C+++ +DD+ F   +PK+   L    S + + Y   + + +L AAPING +AL+ Y+ F
Sbjct: 105 ACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGF 164

Query: 131 IEDAVVEGLEEWGSM--KGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIG 190
           I+D VV  L  W      G    L E +R+TF+II+ +F+G    +   AELE  Y E+ 
Sbjct: 165 IDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFMGGA-DERTAAELERTYTELN 224

Query: 191 PAIMSIFPYNLPGFSYRRALKVSSRNLEVNDRGYIREEKQSREG 230
              M     +LPGF+Y +A++   R +    R  + +E+++R G
Sbjct: 225 YG-MRAMAIDLPGFAYHKAIRARRRLVAALQR--VLDERRARGG 263

[Close]

BLAST of Moc06g35670 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 8.7e-38
Identity = 80/208 (38.46%), Postives = 125/208 (60.10%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPG+MGWP+VGS  +F +AFK  G+P++ + + I RFGR  +Y++ ++  P I+   PE
Sbjct: 55  LPPGEMGWPMVGSMWAFLRAFK-SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPE 114

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVEF 130
            C+++ +DDE F   +PK+   L    S + +   D++ + +L AAPING +AL+ Y+ F
Sbjct: 115 ACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSF 174

Query: 131 IEDAVVEGLEEWGSMK-GPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGP 190
           I+  VV  L  W S + G V  L E +R+TFKII+ +F+     D+ +  LE  Y ++  
Sbjct: 175 IDQTVVASLRRWSSPESGQVEFLTELRRMTFKIIVQIFMSGA-DDATMEALERSYTDLNY 234

Query: 191 AIMSIFPYNLPGFSYRRALKVSSRNLEV 215
             M     NLPGF+Y RAL+   + + V
Sbjct: 235 G-MRAMAINLPGFAYYRALRARRKLVSV 259

[Close]

BLAST of Moc06g35670 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.5e-37
Identity = 87/225 (38.67%), Postives = 135/225 (60.00%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPGD+GWP +G+ LSF +AFK   DP+S    LI R+G   +YK+H++G P IIVT  +
Sbjct: 45  LPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSD 104

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVEF 130
            CRR+  DD+ FK  +P S   L    S + +   ++K + RL AAP+NG EALS Y+ +
Sbjct: 105 TCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPY 164

Query: 131 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPA 190
           IE+ V+  L++W  M G    L   ++LTF+II+ +FL S  +++ +  LE  Y  +   
Sbjct: 165 IEENVITVLDKWTKM-GEFEFLTHLRKLTFRIIMYIFLSSE-SENVMDALEREYTALNYG 224

Query: 191 IMSIFPYNLPGFSYRRALKVSSRNLEVNDRGYIREEKQSREGYML 233
           + ++   N+PGF+Y RALK + + L    +  + E +  R+  +L
Sbjct: 225 VRAM-AVNIPGFAYHRALK-ARKTLVAAFQSIVTERRNQRKQNIL 264

[Close]

BLAST of Moc06g35670 vs. ExPASy TrEMBL
Match: A0A6J1DWI6 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111024712 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.6e-114
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MSRSFLVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYG 60
           MSRSFLVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYG
Sbjct: 1   MSRSFLVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYG 60

Query: 61  FPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEA 120
           FPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEA
Sbjct: 61  FPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEA 120

Query: 121 LSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESL 180
           LSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLG VFTDSEVAELESL
Sbjct: 121 LSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGXVFTDSEVAELESL 180

Query: 181 YAEIGPAIMSIFPYNLPGFSYRRALKV 208
           YAEIGPAIMSIFPYNLPGFSYRRALKV
Sbjct: 181 YAEIGPAIMSIFPYNLPGFSYRRALKV 207

[Close]

BLAST of Moc06g35670 vs. ExPASy TrEMBL
Match: A0A5K7U6A4 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.3e-110
Identity = 203/222 (91.44%), Postives = 209/222 (94.14%), Query Frame = 0


Query: 7   VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIV 66
           VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPN+VMDNLISRFGRVRMYKSHLYGFPVIIV
Sbjct: 41  VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIV 100

Query: 67  TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 126
           TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE
Sbjct: 101 TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 160

Query: 127 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGP 186
           FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLG VFTDSEVAELESLYAEIGP
Sbjct: 161 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGP 220

Query: 187 AIMSIFPYNLPGFSYRRALKVSSRNLEVNDRGYIREEKQSRE 229
           AIMSIFPYNLPGFSYRRALK   + +E   R  I+  K++ E
Sbjct: 221 AIMSIFPYNLPGFSYRRALKARIK-VENIVRPVIQNRKKALE 261

[Close]

BLAST of Moc06g35670 vs. ExPASy TrEMBL
Match: A0A6J1DVM6 (beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.3e-92
Identity = 163/204 (79.90%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 7   VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIV 66
           +YNALPPG MGWPL+GSSLSFY+AFKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIV
Sbjct: 41  IYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIV 100

Query: 67  TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 126
           TDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ 
Sbjct: 101 TDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMG 160

Query: 127 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGP 186
           FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLG  FTDSE+A+LE+LYAEIG 
Sbjct: 161 FIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGL 220

Query: 187 AIMSIFPYNLPGFSYRRALKVSSR 211
           AI+SIFPY+LPGFSYRRALK  S+
Sbjct: 221 AILSIFPYDLPGFSYRRALKARSK 244

[Close]

BLAST of Moc06g35670 vs. ExPASy TrEMBL
Match: A0A5K7U6M8 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.3e-92
Identity = 163/204 (79.90%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 7   VYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIV 66
           +YNALPPG MGWPL+GSSLSFY+AFKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIV
Sbjct: 41  IYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIV 100

Query: 67  TDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVE 126
           TDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ 
Sbjct: 101 TDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMG 160

Query: 127 FIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGP 186
           FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLG  FTDSE+A+LE+LYAEIG 
Sbjct: 161 FIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGL 220

Query: 187 AIMSIFPYNLPGFSYRRALKVSSR 211
           AI+SIFPY+LPGFSYRRALK  S+
Sbjct: 221 AILSIFPYDLPGFSYRRALKARSK 244

[Close]

BLAST of Moc06g35670 vs. ExPASy TrEMBL
Match: A0A6J1DX36 (beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 2.1e-87
Identity = 156/195 (80.00%), Postives = 180/195 (92.31%), Query Frame = 0


Query: 16  MGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRI 75
           MGWPL+GSSLSFY+AFKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 76  YLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEG 135
           YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+G
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 136 LEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPAIMSIFPYN 195
           LEEWGS+KGPV LL EFKRLTFK+I GVFLG  FTDSE+A+LE+LYAEIG AI+SIFPY+
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 196 LPGFSYRRALKVSSR 211
           LPGFSYRRALK  S+
Sbjct: 181 LPGFSYRRALKARSK 195

[Close]

BLAST of Moc06g35670 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 171.8 bits (434), Expect = 7.1e-43
Identity = 84/203 (41.38%), Postives = 130/203 (64.04%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPGD+GWP++G+  SF +AFK   DP S + + I+R+GR  +YK+H++G+P ++VT PE
Sbjct: 44  LPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPE 103

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVEF 130
            CRR+  DD+ F + +PKS   L    S + +   ++K + RL +AP+NG EALS Y++F
Sbjct: 104 TCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQF 163

Query: 131 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPA 190
           IE+ V   LE+W  M G +  L   ++LTFK+I+ +FL S  ++  +  LE  Y  +   
Sbjct: 164 IEETVNTDLEKWSKM-GEIEFLSHLRKLTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYG 223

Query: 191 IMSIFPYNLPGFSYRRALKVSSR 211
           + ++   NLPGF+Y RALK   +
Sbjct: 224 VRAM-GINLPGFAYHRALKARKK 242

[Close]

BLAST of Moc06g35670 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 171.8 bits (434), Expect = 7.1e-43
Identity = 84/203 (41.38%), Postives = 130/203 (64.04%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPGD+GWP++G+  SF +AFK   DP S + + I+R+GR  +YK+H++G+P ++VT PE
Sbjct: 44  LPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPE 103

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVEF 130
            CRR+  DD+ F + +PKS   L    S + +   ++K + RL +AP+NG EALS Y++F
Sbjct: 104 TCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQF 163

Query: 131 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPA 190
           IE+ V   LE+W  M G +  L   ++LTFK+I+ +FL S  ++  +  LE  Y  +   
Sbjct: 164 IEETVNTDLEKWSKM-GEIEFLSHLRKLTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYG 223

Query: 191 IMSIFPYNLPGFSYRRALKVSSR 211
           + ++   NLPGF+Y RALK   +
Sbjct: 224 VRAM-GINLPGFAYHRALKARKK 242

[Close]

BLAST of Moc06g35670 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 157.1 bits (396), Expect = 1.8e-38
Identity = 87/225 (38.67%), Postives = 135/225 (60.00%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPGD+GWP +G+ LSF +AFK   DP+S    LI R+G   +YK+H++G P IIVT  +
Sbjct: 45  LPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSD 104

Query: 71  VCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVEF 130
            CRR+  DD+ FK  +P S   L    S + +   ++K + RL AAP+NG EALS Y+ +
Sbjct: 105 TCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPY 164

Query: 131 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPA 190
           IE+ V+  L++W  M G    L   ++LTF+II+ +FL S  +++ +  LE  Y  +   
Sbjct: 165 IEENVITVLDKWTKM-GEFEFLTHLRKLTFRIIMYIFLSSE-SENVMDALEREYTALNYG 224

Query: 191 IMSIFPYNLPGFSYRRALKVSSRNLEVNDRGYIREEKQSREGYML 233
           + ++   N+PGF+Y RALK + + L    +  + E +  R+  +L
Sbjct: 225 VRAM-AVNIPGFAYHRALK-ARKTLVAAFQSIVTERRNQRKQNIL 264

[Close]

BLAST of Moc06g35670 vs. TAIR 10
Match: AT1G73340.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 7.1e-19
Identity = 57/207 (27.54%), Postives = 115/207 (55.56%), Query Frame = 0


Query: 11  LPPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPE 70
           LPPG  GWPL+G + ++  A   G  P+S ++  I ++GR+  +   L+G   ++  DP+
Sbjct: 43  LPPGSRGWPLIGDTFAWLNAV-AGSHPSSFVEKQIKKYGRI--FSCSLFGKWAVVSADPD 102

Query: 71  VCRRIYLDD-EHFKLNYPKSVKLLQADGSLLKV---DYKIVHRLMAAPINGVEALSRYVE 130
             R I  ++ + F+ +YPKS + L     ++ V     + +H + ++ +   +  + ++E
Sbjct: 103 FNRFIMQNEGKLFQSSYPKSFRDLVGKDGVITVHGDQQRRLHSIASSMMRHDQLKTHFLE 162

Query: 131 FIEDAVVEGLEEWGSMKGPVPLLHEF-KRLTFKIIIGVFLGSVFTDSEVAELESLYAEIG 190
            I   +++ L  +    G V LL +  +++   +++   LG V ++SEV E+  L+++  
Sbjct: 163 VIPVVMLQTLSNF--KDGEVVLLQDICRKVAIHLMVNQLLG-VSSESEVDEMSQLFSDFV 222

Query: 191 PAIMSIFPYNLPGFSYRRALKVSSRNL 213
              +S+ P +LPGF+Y +A+KVS ++L
Sbjct: 223 DGCLSV-PIDLPGFTYNKAMKVSFKHL 242

[Close]

BLAST of Moc06g35670 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 85.9 bits (211), Expect = 5.1e-17
Identity = 64/231 (27.71%), Postives = 110/231 (47.62%), Query Frame = 0


Query: 12  PPGDMGWPLVGSSLSFYQAFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEV 71
           P G +GWP++G ++ F  +      P S MD     +GRV  +KSH++G   I+ TD EV
Sbjct: 52  PHGSLGWPVIGETIEFVSS-AYSDRPESFMDKRRLMYGRV--FKSHIFGTATIVSTDAEV 111

Query: 72  CRRIYLDDE-HFKLNYPKSVKLLQADGSLLKVD---YKIVHRLMAAPINGVEALSRYVEF 131
            R +   D   F   YPK+V+ L    S+L ++   ++  H L+ + +      ++ V  
Sbjct: 112 NRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRD 171

Query: 132 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGSVFTDSEVAELESLYAEIGPA 191
           +   + E ++ W S   PV L    K + FK++    + SV    ++ EL+  +      
Sbjct: 172 MHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALI-SVEKGEDLEELKREFENFISG 231

Query: 192 IMSIFPYNLPGFSYRRALKVSSRNLEVNDR---GYIREEKQSREGYMLKAD 236
           +MS+ P N PG    R+L+     ++  +R   G IR+ K   E  ++  D
Sbjct: 232 LMSL-PINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKD 276

The following BLAST results are available for this feature:

BLAST of Moc06g35670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158172.1	3.2e-114	99.52	beta-amyrin 11-oxidase-like [Momordica charantia]	[more]
XP_022157153.1	4.8e-110	91.44	beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 mo...	[more]
XP_022157134.1	2.7e-92	79.90	beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia]	[more]
BBI21930.1	2.7e-92	79.90	cytochrome P450 monooxygenase [Momordica charantia]	[more]
XP_022157141.1	4.4e-87	80.00	beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia]	[more]

BLAST of Moc06g35670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	2.4e-43	43.35	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	1.0e-41	41.38	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q9AXH9	8.7e-38	37.50	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	8.7e-38	38.46	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
O23051	2.5e-37	38.67	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]

BLAST of Moc06g35670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWI6	1.6e-114	99.52	beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111024712 PE=4 ...	[more]
A0A5K7U6A4	2.3e-110	91.44	Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 S...	[more]
A0A6J1DVM6	1.3e-92	79.90	beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5K7U6M8	1.3e-92	79.90	Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 S...	[more]
A0A6J1DX36	2.1e-87	80.00	beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Moc06g35670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	7.1e-43	41.38	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	7.1e-43	41.38	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	1.8e-38	38.67	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT1G73340.1	7.1e-19	27.54	Cytochrome P450 superfamily protein	[more]
AT3G13730.1	5.1e-17	27.71	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 6..239 e-value: 4.7E-25 score: 90.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 10..213
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 6..229
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 6..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g35670.1	Moc06g35670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen