Moc06g33680 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g33680
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUBR-type domain-containing protein
Locationchr6: 25528997 .. 25531439 (-)
RNA-Seq ExpressionMoc06g33680
SyntenyMoc06g33680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAAGTTTTCGATGATGAAACCGAACAGACGATTTCTATTCAGGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGTCTCTCTCTCTCTCTCTCTGTCCTTCTCTCTTTTTTTGAATTAGGGTGTACGAGTATTGGTTGGTTTTCCAAGAATTTAGCGATGTAATGGCCTGCAAACGTTCTGGGTTTTCTATAATTAGGGTTCGTGGCGTGTAATTCTTGAAAATATTCGTTTGTGTGCGCGAGTAGTGAGAGTATGAATTAGGAACTCAGTTACAACTGTAATCAAAACATCTTAATCTCGACTGTCCTAAAATTTTCGTTTAGCGGAGGTCATCCTGACTTTCTACAGAGAATATCACTTTACTATCTTTTGTTCTTGTTTCTGTATCTTCTTTGTGGGTGCATGGGATCTGATTATTGTTTTTTATTATAAAGGAAGCGGATTTAGTTTTGGGAGGTGACGAAGGGAAAGAATGTACTTACAGCAAGGGCTATATGAAGAGACAAGCCATTTTCTCATGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACAGCATGTAGCTTGTCATGCCATGATGGGCACGAGGTATACATGTTTTGAAAAAGAACTCTCTTTATTATTTGCAGTGATTTAAGTTTCTTTTGTCCAACGTTTGCTGCTGTTGTGCCAAGCTATAGACCGTGTCTCTGATTGCTTTTGAGCAGTATAATTTGTAAAGTATGGAACATATATTTTGTCACCATATTTTACTGAGTCTTTTTTGACACACCAAGTTTTTGGAGAAGATCCAGCCTGCTGTATGTGGCTCTGAAGAAGCGTTTAGTGGATTTTTCATGTTTAAACTCTAGGTTATTGCGTTATGAACATAACGTTTTGTTATTGATTGTTATTCCCAAGTTGCATAAATGAATATCAATAATTTATTTAAATTGTATGACTTCATTCCAATGATGTTAATTTCTCTCCGAGGACTTTGCTTACTAACCCTTCTTATCTTATCATGGGTATTGTTTTCTTGGGAAAGATTGTGGAACTATGGACCAAAAGGAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCTTCTGCAAGCTGACCTCAAACAAAGCTGTAGAAAATGCCAACAATTCGTATAATCATAATTTTAAAGGATCATATTGCATATGCAATCGCCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATTCAGTGCTGTTTGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAATTCCATAGATGAGGTGTGTTAGCATGAAAACCTTCCGTGGGTTTTCAGTTCTTGTCTTGTTTCTCCATTTTTGTTGTTGATAATCACTAGAACAGTCTCCTCGAGATGGATAATTAAGCAAGATCTGCAAGTGATAATCTGTTGAATGTGAAGCTGAGTAAATTTTTATCCTCACAAGAAAATTACCTTGCCTTTTCTCTGTGATGATGGAACTTTCTTACCGGTGGATTTAAAATTCCTACTTGTTGATTGTTATTTAACTGCATCGATTATCTATCTATCACTTGTTCTGCAGATTCCAAGGGATGAAGAAGGAGTGCCCCAGTATGAGGATTTCATATGCAAGGCATGTTCTGCAGTTTGTTCTTTTCTCTGTCAATATCCTTCGTCTATTTGGGCAGTGGGAAGGCACCCTGATATTTCCTTGGACGTTAGCGTGAAGAATGATGTTCCAGAACAGACCCTTTCAGTTTGTACGTCTGTAAAGCATGATAATGATGGTAATCCCGATGATTCTGCTAACGCAGACCTTACAGTAGCTCCAGAAGAATCTGCTGGAAAAGATAGCTTTCTTAGTGAACTTCCTGAGAATAATTCTTGTTCTAATGAAACTATTAACGAAGTCGATCAAACTGCTAGTTGTGTGCTTGGAACTTCTCAGTCCTCTGCTTCCGTGGTTGTAGAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCTTTGTGCAGATGTGAGAAATGCATCAACAACTACAAGCTGAAGAATATTAGTTTTCTGCTAGATGAAGAGGACTCCATTGCCGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGCAACAAGAGGGTGCTGAGTTGAAGATGTTTGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTAAGTGGCATCGCGGATATGAAGGACGAGATTCGTACATTTCTGGTATTCTCTCAGTCACTCGTTTTCACATATACAATGAAGAACCATATCAATGCATGGTTGCATGTTCTTCGTATTTTGCATGGTTATATGTCTTTTCTTCTTTGCAGGAATCGTTCGACTCGTCAAAGCCTATTACATCTGCTGATATACACCAGGTTTTCGAGAACTTAGCTAAAAAGAGGCGTCGTACTGAATGA

mRNA sequence

ATGGCGGAAGTTTTCGATGATGAAACCGAACAGACGATTTCTATTCAGGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGAAGCGGATTTAGTTTTGGGAGGTGACGAAGGGAAAGAATGTACTTACAGCAAGGGCTATATGAAGAGACAAGCCATTTTCTCATGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACAGCATGTAGCTTGTCATGCCATGATGGGCACGAGATTGTGGAACTATGGACCAAAAGGAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCTTCTGCAAGCTGACCTCAAACAAAGCTGTAGAAAATGCCAACAATTCGTATAATCATAATTTTAAAGGATCATATTGCATATGCAATCGCCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATTCAGTGCTGTTTGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAATTCCATAGATGAGATTCCAAGGGATGAAGAAGGAGTGCCCCAGTATGAGGATTTCATATGCAAGGCATGTTCTGCAGTTTGTTCTTTTCTCTGTCAATATCCTTCGTCTATTTGGGCAGTGGGAAGGCACCCTGATATTTCCTTGGACGTTAGCGTGAAGAATGATGTTCCAGAACAGACCCTTTCAGTTTGTACGTCTGTAAAGCATGATAATGATGGTAATCCCGATGATTCTGCTAACGCAGACCTTACAGTAGCTCCAGAAGAATCTGCTGGAAAAGATAGCTTTCTTAGTGAACTTCCTGAGAATAATTCTTGTTCTAATGAAACTATTAACGAAGTCGATCAAACTGCTAGTTGTGTGCTTGGAACTTCTCAGTCCTCTGCTTCCGTGGTTGTAGAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCTTTGTGCAGATGTGAGAAATGCATCAACAACTACAAGCTGAAGAATATTAGTTTTCTGCTAGATGAAGAGGACTCCATTGCCGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGCAACAAGAGGGTGCTGAGTTGAAGATGTTTGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTAAGTGGCATCGCGGATATGAAGGACGAGATTCGTACATTTCTGGAATCGTTCGACTCGTCAAAGCCTATTACATCTGCTGATATACACCAGGTTTTCGAGAACTTAGCTAAAAAGAGGCGTCGTACTGAATGA

Coding sequence (CDS)

ATGGCGGAAGTTTTCGATGATGAAACCGAACAGACGATTTCTATTCAGGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGAAGCGGATTTAGTTTTGGGAGGTGACGAAGGGAAAGAATGTACTTACAGCAAGGGCTATATGAAGAGACAAGCCATTTTCTCATGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACAGCATGTAGCTTGTCATGCCATGATGGGCACGAGATTGTGGAACTATGGACCAAAAGGAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCTTCTGCAAGCTGACCTCAAACAAAGCTGTAGAAAATGCCAACAATTCGTATAATCATAATTTTAAAGGATCATATTGCATATGCAATCGCCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATTCAGTGCTGTTTGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAATTCCATAGATGAGATTCCAAGGGATGAAGAAGGAGTGCCCCAGTATGAGGATTTCATATGCAAGGCATGTTCTGCAGTTTGTTCTTTTCTCTGTCAATATCCTTCGTCTATTTGGGCAGTGGGAAGGCACCCTGATATTTCCTTGGACGTTAGCGTGAAGAATGATGTTCCAGAACAGACCCTTTCAGTTTGTACGTCTGTAAAGCATGATAATGATGGTAATCCCGATGATTCTGCTAACGCAGACCTTACAGTAGCTCCAGAAGAATCTGCTGGAAAAGATAGCTTTCTTAGTGAACTTCCTGAGAATAATTCTTGTTCTAATGAAACTATTAACGAAGTCGATCAAACTGCTAGTTGTGTGCTTGGAACTTCTCAGTCCTCTGCTTCCGTGGTTGTAGAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCTTTGTGCAGATGTGAGAAATGCATCAACAACTACAAGCTGAAGAATATTAGTTTTCTGCTAGATGAAGAGGACTCCATTGCCGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGCAACAAGAGGGTGCTGAGTTGAAGATGTTTGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTAAGTGGCATCGCGGATATGAAGGACGAGATTCGTACATTTCTGGAATCGTTCGACTCGTCAAAGCCTATTACATCTGCTGATATACACCAGGTTTTCGAGAACTTAGCTAAAAAGAGGCGTCGTACTGAATGA

Protein sequence

MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYNHNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYEDFICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDDSANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKPMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELKMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE
Homology
BLAST of Moc06g33680 vs. NCBI nr
Match: XP_022146039.1 (putative E3 ubiquitin-protein ligase UBR7 [Momordica charantia])

HSP 1 Score: 852.8 bits (2202), Expect = 1.2e-243
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD
Sbjct: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240

Query: 241 SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP 300
           SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP
Sbjct: 241 SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP 300

Query: 301 MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK 360
           MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK
Sbjct: 301 MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK 360

Query: 361 MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 417

BLAST of Moc06g33680 vs. NCBI nr
Match: KAG6605576.1 (putative E3 ubiquitin-protein ligase UBR7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741.5 bits (1913), Expect = 4.0e-210
Identity = 364/418 (87.08%), Postives = 385/418 (92.11%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGY+KRQAIFSCLTCT
Sbjct: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDF CKL SNK VENANNSYN
Sbjct: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNF+GSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDEIPRDE+G P YED
Sbjct: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FIC ACSAVCSFL QYPSSIWAVGR  DISLDV++KND PE  LS+ TSVKHD+D N  D
Sbjct: 228 FICSACSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287

Query: 241 SANADLTVAPEESAGKDSFLSE-LPENNSCSNETINEVDQTASCVLGTSQSSASVVVENK 300
           SAN DLTVAP++SA K+S LSE   ENN  SNE+I +  QT SCVLG SQSSAS+ V+NK
Sbjct: 288 SANVDLTVAPDKSADKNSLLSEPTTENNRSSNESIKDNSQTVSCVLGASQSSASIAVQNK 347

Query: 301 PMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360
           PMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 348 PMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 407

Query: 361 KMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           K+FDNLGHVEKIEIL+GIADMKDEI TFLESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 408 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 465

BLAST of Moc06g33680 vs. NCBI nr
Match: XP_038876257.1 (putative E3 ubiquitin-protein ligase UBR7 [Benincasa hispida])

HSP 1 Score: 738.8 bits (1906), Expect = 2.6e-209
Identity = 366/417 (87.77%), Postives = 384/417 (92.09%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDF CKL SNK VEN+NNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFVCKLFSNKDVENSNNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL++IDEIPRDEEG P YED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSTIDEIPRDEEGEPLYED 180

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FIC ACSAVCSFL QYP SIW+VGR  DISLD S KND PE TL   TSVKHD D NP D
Sbjct: 181 FICSACSAVCSFLSQYPLSIWSVGRQSDISLDASNKNDAPEHTLPASTSVKHD-DVNPHD 240

Query: 241 SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP 300
           SAN D TVA ++SAGKDS LSELPE+NS S+E+I + +QT SCVLGTS SSAS++VENKP
Sbjct: 241 SANVDFTVATDKSAGKDSMLSELPEHNSSSSESIKDNNQTDSCVLGTS-SSASIIVENKP 300

Query: 301 MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK 360
           MFL KNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEK+AKQKREEKL QQEGAE K
Sbjct: 301 MFLLKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKMAKQKREEKLLQQEGAESK 360

Query: 361 MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           +FDNLGHVEKIEIL+GIADMK+EI TFLESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 LFDNLGHVEKIEILNGIADMKNEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 415

BLAST of Moc06g33680 vs. NCBI nr
Match: KAG7035491.1 (putative E3 ubiquitin-protein ligase UBR7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 736.1 bits (1899), Expect = 1.7e-208
Identity = 361/418 (86.36%), Postives = 384/418 (91.87%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGY+KRQAIFSCLTCT
Sbjct: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDF CKL SNK VENANNSYN
Sbjct: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNF+GSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDEIPRDE+G P YED
Sbjct: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FIC  CSAVCSFL QYPSSIWAVGR  DISLDV++KND PE  LS+ TSVKHD+D N  D
Sbjct: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287

Query: 241 SANADLTVAPEESAGKDSFLSE-LPENNSCSNETINEVDQTASCVLGTSQSSASVVVENK 300
           SAN DLTVAP++SA K+S LSE   EN+  SNE+I +  QT SCVLG SQSSAS+ V+NK
Sbjct: 288 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 347

Query: 301 PMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360
           PMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 348 PMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 407

Query: 361 KMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           K+FDNLGHVEKIEIL+GIADMKDEI TFLESFDS+KPITSADIHQVFENLAKKRRRTE
Sbjct: 408 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE 465

BLAST of Moc06g33680 vs. NCBI nr
Match: XP_023534431.1 (putative E3 ubiquitin-protein ligase UBR7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 736.1 bits (1899), Expect = 1.7e-208
Identity = 364/418 (87.08%), Postives = 382/418 (91.39%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGY+KRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDF CKL SNK VENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDEIPRDE+G P YED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 180

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FIC ACSAVCSFL QYPSSIWAVGR  DISLDV+ KND PE  LS  TSVK D+D N  D
Sbjct: 181 FICSACSAVCSFLSQYPSSIWAVGRPSDISLDVTKKNDAPEPILSHSTSVKRDDDANAHD 240

Query: 241 SANADLTVAPEESAGKDSFLSE-LPENNSCSNETINEVDQTASCVLGTSQSSASVVVENK 300
           SAN DLTVAP++SA K+S LSE   EN+  SNE+I + DQT SCVLG SQSSAS  V+NK
Sbjct: 241 SANVDLTVAPDKSADKNSLLSEPTTENDRGSNESIKDNDQTVSCVLGASQSSASTAVQNK 300

Query: 301 PMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360
           PMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 301 PMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360

Query: 361 KMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           K+FDNLGHVEKIEIL+GIADMKDEI TFLESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418

BLAST of Moc06g33680 vs. ExPASy Swiss-Prot
Match: Q8BU04 (Putative E3 ubiquitin-protein ligase UBR7 OS=Mus musculus OX=10090 GN=Ubr7 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.6e-65
Identity = 157/425 (36.94%), Postives = 230/425 (54.12%), Query Frame = 0

Query: 8   ETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGN--A 67
           E E  +S+ + L+E EE E EA  VLGG + ++C+YS+G + RQA+++C TCTP+G   A
Sbjct: 13  ELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVGRQALYACSTCTPEGEEPA 72

Query: 68  GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYNHNFKG 127
           G+C ACS  CH  H++ EL+TKRNFRCDCGNSKF +  CKL  +K+  N+ N YN NF G
Sbjct: 73  GICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLFPDKSKVNSCNKYNDNFFG 132

Query: 128 SYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYEDFICKA 187
            YC+C RPYPDP+ E   EMIQC +CEDWFH  HLG      IP  E G   +++ +C+A
Sbjct: 133 LYCVCKRPYPDPEDEVPDEMIQCVVCEDWFHGRHLG-----AIP-PESG--DFQEMVCQA 192

Query: 188 CSAVCSFLCQYPSSIWAVGRHPDISLDVSVKND--VPEQTLSVCTSVKHDNDGNPDDSAN 247
           C   CSFL  Y + + AV R       +S ++D  +P  T      V    +G P D  N
Sbjct: 193 CMRRCSFLWAYAAQL-AVTR-------ISAEDDGLLPNATGMGDEDVSKPENGAPQD--N 252

Query: 248 ADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQT------------ASCVLGTSQSS 307
                APE      + +    +N  CS+ +     QT            +SC L   Q+ 
Sbjct: 253 GLKEDAPEHGRDSVNEVKAEQKNEPCSSSSSESDLQTVFKKENIKTEPQSSCRLQELQAK 312

Query: 308 ASVVVENKPMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKL 367
              V ++   +   NWR  LC C+ C+  Y   ++ FL DE D++  YE   K K ++  
Sbjct: 313 -QFVKKDAATYWPLNWRSKLCTCQDCMKMYGELDVLFLTDECDTVLAYEN--KGKNDQAT 372

Query: 368 QQQEGAELKMFDNLGHVEKIEILSGIADMKDEIRTFLESF-DSSKPITSADIHQVFENLA 416
            +++   +    ++  V+++E++    D+K E++ +L+ F D    +   DI Q FE   
Sbjct: 373 DRRDPL-MDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQ 415

BLAST of Moc06g33680 vs. ExPASy Swiss-Prot
Match: Q8N806 (Putative E3 ubiquitin-protein ligase UBR7 OS=Homo sapiens OX=9606 GN=UBR7 PE=1 SV=2)

HSP 1 Score: 251.1 bits (640), Expect = 2.1e-65
Identity = 154/426 (36.15%), Postives = 229/426 (53.76%), Query Frame = 0

Query: 8   ETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGN--A 67
           E E  +S+ + L+E EE E EA  VLGG + ++C+YS+G +KRQA+++C TCTP+G   A
Sbjct: 13  ELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVKRQALYACSTCTPEGEEPA 72

Query: 68  GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYNHNFKG 127
           G+C ACS  CH  H++ EL+TKRNFRCDCGNSKF +  CKL  +KA  N+ N YN NF G
Sbjct: 73  GICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLLPDKAKVNSGNKYNDNFFG 132

Query: 128 SYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYEDFICKA 187
            YCIC RPYPDP+ E   EMIQC +CEDWFH  HLG      IP  E G   +++ +C+A
Sbjct: 133 LYCICKRPYPDPEDEIPDEMIQCVVCEDWFHGRHLG-----AIP-PESG--DFQEMVCQA 192

Query: 188 CSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDDSANAD 247
           C   CSFL  Y + +       +    V   + + +Q +     +K +N  + D +   D
Sbjct: 193 CMKRCSFLWAYAAQLAVTKISTEDDGLVRNIDGIGDQEV-----IKPENGEHQDSTLKED 252

Query: 248 LTVAPEESAGKDSFLSELPENNS--CS-------------NETINEVDQTASCVLGTSQS 307
           +   PE+  GKD       E NS  C+             NE++N  +  + C L     
Sbjct: 253 V---PEQ--GKDDVREVKVEQNSEPCAGSSSESDLQTVFKNESLN-AESKSGCKL-QELK 312

Query: 308 SASVVVENKPMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEK 367
           +  ++ ++   +   NWR  LC C+ C+  Y   ++ FL DE D++  YE   K     +
Sbjct: 313 AKQLIKKDTATYWPLNWRSKLCTCQDCMKMYGDLDVLFLTDEYDTVLAYENKGK---IAQ 372

Query: 368 LQQQEGAELKMFDNLGHVEKIEILSGIADMKDEIRTFLESF-DSSKPITSADIHQVFENL 416
              +    +    ++  V+++E++    D+K E++ +L+ F D    +   DI Q FE  
Sbjct: 373 ATDRSDPLMDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEF 415

BLAST of Moc06g33680 vs. ExPASy Swiss-Prot
Match: Q54DV0 (Putative E3 ubiquitin-protein ligase ubr7 OS=Dictyostelium discoideum OX=44689 GN=ubr7 PE=3 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-32
Identity = 131/465 (28.17%), Postives = 194/465 (41.72%), Query Frame = 0

Query: 3   EVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTC--T 62
           E  +D+ E+  ++ E  KE E+++        GDE   CT+ KGY+  Q++F+C TC  +
Sbjct: 72  EALNDQLEEEKNLLEEAKEQEQED-------WGDE-SICTFDKGYI-NQSVFACKTCQLS 131

Query: 63  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCG---NSKFGDFFCKLT------SNKA 122
            D   G C  CS+ CH  H++ EL+ KRNFRCDCG         F C+L+       N  
Sbjct: 132 NDKLFGFCYGCSMHCHLYHDVYELFNKRNFRCDCGTKIQEPNNSFKCQLSGILKEDDNNN 191

Query: 123 VENANNS-----------------------------------------YNHNFKGSYCIC 182
           V N NNS                                         YNHNFKG YC C
Sbjct: 192 VNNINNSNNTTTTTTTTTTTTTTTNNNHLNDIDIGSYDKSQILNERNHYNHNFKGKYCYC 251

Query: 183 NRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNS-IDEIPRDEEGVPQYEDFICKACSAV 242
           + PY   D +E  +MIQC  CEDWFHE  L LNS + +IP   E    + D IC  C + 
Sbjct: 252 DSPY---DYKE--DMIQCIFCEDWFHENCLKLNSNVTDIPSPGE----FSDLICADCLSK 311

Query: 243 CSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDDSANADLTVA 302
             FL  YP             +   ++ND           +  DN+ N ++S N+     
Sbjct: 312 NQFLLLYP------------QIRCYIEND---------HIIIGDNNNNNNNSNNS----- 371

Query: 303 PEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENK--PMFLSKNW 362
                           N+ C  E                     V+  NK   +F  + W
Sbjct: 372 ----------------NSICKVE-------------------GGVITPNKKYDLFCKELW 431

Query: 363 RGALCRCEKCINNYKLKNISFLLD-EEDSIAEYEKIAKQKREEK-LQQQEGAELKMFDNL 410
           +  LC C KC   YK K + FL + +E+S+ +  K   +  + K +   E  +      L
Sbjct: 432 KDELCSCLKCKEIYKDKKVEFLFEKDENSLKKKNKTVDENLDNKPVNVFEMGQDVFSKTL 457

BLAST of Moc06g33680 vs. ExPASy Swiss-Prot
Match: Q09329 (Protein mlo2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mlo2 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.5e-30
Identity = 97/275 (35.27%), Postives = 144/275 (52.36%), Query Frame = 0

Query: 7   DETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGNA- 66
           +ET   +++++Y+++  E E EA  VL       CTYS GY+K Q +++CLTC     + 
Sbjct: 2   EETAHELTVKQYVEQQRELEREAREVL-PYSFDTCTYSMGYLK-QPLYACLTCQKASGSL 61

Query: 67  -GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYNHNFK 126
             VC +CS+SCH  H++V+L+ KR+FRCDCG ++     C L  +     + N YNHNF+
Sbjct: 62  NAVCYSCSISCHADHDLVDLFNKRHFRCDCGTTRTHSIPCNLRKSVDECGSENDYNHNFE 121

Query: 127 GSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYEDFICK 186
           G +CIC+  Y +P+ EE   M QC LCEDWFHE+ L   +      D E    +E  +C 
Sbjct: 122 GRFCICDTVY-NPETEEGT-MFQCILCEDWFHEKCLQKTNKGIAIPDAE---TFEWLVCS 181

Query: 187 ACSAVC--SFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDDSA 246
            CS       L Q   SI    R P    +   +N  P ++   C S++  N   P   A
Sbjct: 182 ECSEKYRDHLLNQKHESIAGTERAPLFLSENFRENLCPCES---CISLR--NLEMPMLVA 241

Query: 247 NADLTVAPEESAGKDSFLSELPENNSCSNETINEV 278
              +   PE+S   +  +SE+ E+ S S E I +V
Sbjct: 242 EEPIYEPPEDS---EDGISEMNEDPSESGEMIEQV 261

BLAST of Moc06g33680 vs. ExPASy TrEMBL
Match: A0A6J1CWZ9 (putative E3 ubiquitin-protein ligase UBR7 OS=Momordica charantia OX=3673 GN=LOC111015345 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 5.9e-244
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD
Sbjct: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240

Query: 241 SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP 300
           SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP
Sbjct: 241 SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP 300

Query: 301 MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK 360
           MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK
Sbjct: 301 MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK 360

Query: 361 MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 417

BLAST of Moc06g33680 vs. ExPASy TrEMBL
Match: A0A6J1H3N3 (putative E3 ubiquitin-protein ligase UBR7 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459805 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.1e-208
Identity = 360/418 (86.12%), Postives = 383/418 (91.63%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGY+KRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNS FGDF CKL SNK VENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSNFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDEIPRDE+G P YED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 180

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FIC  CSAVCSFL QYPSSIWAVGR  DISLDV++KND PE  LS+ TSVKHD+D N  D
Sbjct: 181 FICSTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 240

Query: 241 SANADLTVAPEESAGKDSFLSE-LPENNSCSNETINEVDQTASCVLGTSQSSASVVVENK 300
           SAN DL+VAP++SA K+S L+E   EN+  SNE+I +  QT SCVLG SQSSAS+ V+NK
Sbjct: 241 SANVDLSVAPDKSADKNSLLTEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 300

Query: 301 PMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360
           PMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 301 PMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360

Query: 361 KMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           K+FDNLGHVEKIEIL+GIADMKDEI TFLESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418

BLAST of Moc06g33680 vs. ExPASy TrEMBL
Match: A0A6J1K3N0 (putative E3 ubiquitin-protein ligase UBR7 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491379 PE=4 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 2.6e-207
Identity = 360/418 (86.12%), Postives = 381/418 (91.15%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGY+KRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           P GNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDF CKL SNK VENANNSYN
Sbjct: 61  PAGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180
           HNF GSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDEIPRDE+G P YED
Sbjct: 121 HNFNGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 180

Query: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240
           FIC ACSAVCSFL QYPSSIWAVGR  DISLDV++KND PE  LS+ TSVKHD+D N  D
Sbjct: 181 FICSACSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDANAHD 240

Query: 241 SANADLTVAPEESAGKDSFLSE-LPENNSCSNETINEVDQTASCVLGTSQSSASVVVENK 300
           SAN DLTVAP++SA K+S LSE   EN+  SNE+I +  QT SCVLG SQSSAS  ++NK
Sbjct: 241 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASRAIQNK 300

Query: 301 PMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360
           PMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 301 PMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360

Query: 361 KMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418
           K+FD LGHVEKIEIL+GIADMKDEI TFLESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDKLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418

BLAST of Moc06g33680 vs. ExPASy TrEMBL
Match: A0A6J1H2D9 (putative E3 ubiquitin-protein ligase UBR7 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459805 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.7e-206
Identity = 360/422 (85.31%), Postives = 383/422 (90.76%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGY+KRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNS FGDF CKL SNK VENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSNFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDE----IPRDEEGVP 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDE    IPRDE+G P
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEFLLQIPRDEDGEP 180

Query: 181 QYEDFICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDG 240
            YEDFIC  CSAVCSFL QYPSSIWAVGR  DISLDV++KND PE  LS+ TSVKHD+D 
Sbjct: 181 LYEDFICSTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDT 240

Query: 241 NPDDSANADLTVAPEESAGKDSFLSE-LPENNSCSNETINEVDQTASCVLGTSQSSASVV 300
           N  DSAN DL+VAP++SA K+S L+E   EN+  SNE+I +  QT SCVLG SQSSAS+ 
Sbjct: 241 NAHDSANVDLSVAPDKSADKNSLLTEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIA 300

Query: 301 VENKPMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 360
           V+NKPMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEKIAKQKREEKLQQQE
Sbjct: 301 VQNKPMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 360

Query: 361 GAELKMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRR 418
           GAELK+FDNLGHVEKIEIL+GIADMKDEI TFLESFDSSKPITSADIHQVFENLAKKRRR
Sbjct: 361 GAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRR 420

BLAST of Moc06g33680 vs. ExPASy TrEMBL
Match: A0A6J1K5N1 (putative E3 ubiquitin-protein ligase UBR7 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491379 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 1.4e-205
Identity = 360/422 (85.31%), Postives = 381/422 (90.28%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGY+KRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120
           P GNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDF CKL SNK VENANNSYN
Sbjct: 61  PAGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDE----IPRDEEGVP 180
           HNF GSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDE    IPRDE+G P
Sbjct: 121 HNFNGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEFLLQIPRDEDGEP 180

Query: 181 QYEDFICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDG 240
            YEDFIC ACSAVCSFL QYPSSIWAVGR  DISLDV++KND PE  LS+ TSVKHD+D 
Sbjct: 181 LYEDFICSACSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDA 240

Query: 241 NPDDSANADLTVAPEESAGKDSFLSE-LPENNSCSNETINEVDQTASCVLGTSQSSASVV 300
           N  DSAN DLTVAP++SA K+S LSE   EN+  SNE+I +  QT SCVLG SQSSAS  
Sbjct: 241 NAHDSANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASRA 300

Query: 301 VENKPMFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 360
           ++NKPMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEKIAKQKREEKLQQQE
Sbjct: 301 IQNKPMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 360

Query: 361 GAELKMFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRR 418
           GAELK+FD LGHVEKIEIL+GIADMKDEI TFLESFDSSKPITSADIHQVFENLAKKRRR
Sbjct: 361 GAELKLFDKLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRR 420

BLAST of Moc06g33680 vs. TAIR 10
Match: AT4G23860.1 (PHD finger protein-related )

HSP 1 Score: 463.0 bits (1190), Expect = 2.5e-130
Identity = 239/449 (53.23%), Postives = 300/449 (66.82%), Query Frame = 0

Query: 4   VFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDG 63
           VF+DE E TI+I EY++ ++ +EL ADLVLGGDEG ECT+ KGYMKRQAIFSC+TCTP+G
Sbjct: 5   VFEDEAEGTITINEYIERLDAEELAADLVLGGDEGDECTFPKGYMKRQAIFSCITCTPEG 64

Query: 64  NAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYNHNF 123
           NAG+CTAC LSCHDGHE++ELWTKRNFRCDCGNSKFG   CKL  +K +EN+ NSYNHNF
Sbjct: 65  NAGICTACCLSCHDGHELLELWTKRNFRCDCGNSKFGTLACKLLPSKDIENSENSYNHNF 124

Query: 124 KGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSID----EIPRDEEGVPQYE 183
           KG YC C+RPYPDP+VEEQVEMIQCCLCEDWFHEEHLGL   D    +IPRDEE  P YE
Sbjct: 125 KGLYCTCDRPYPDPNVEEQVEMIQCCLCEDWFHEEHLGLTPSDSVGSQIPRDEESEPIYE 184

Query: 184 DFICKACSAVCSFLCQYPSSIWAVGR----------HPDISLDVSVKNDVPEQ------- 243
           DFIC+ CS  CSFL  YP ++W V +             I LD +  +  P Q       
Sbjct: 185 DFICQNCSPACSFLTLYPENLWVVAKVDSTGSANACSETIELDKNHMDSEPGQPENGTDA 244

Query: 244 ---TLSVCTSVKHDND-GNPDDSANADLTVAPEESAGKDSFLSELPENNS---------C 303
               +  C+    D++ G P++   A+ +V  + S   D   +  PEN++         C
Sbjct: 245 EKSVVGKCSETISDSEPGQPENGTEAEKSVVQKCSEKIDESEAGQPENSTEAEKFVVRKC 304

Query: 304 SNETI-NEVDQTASCVLGTSQSSASVVVENKPMFLSKNWRGALCRCEKCINNYKLKNISF 363
           S +   +E    A CV+ T  +S     E KP+FL+KNWR  LCRCEKC+  YK + +S+
Sbjct: 305 SEKIDGSENVPAAGCVIRTDLNSCP-EFEKKPLFLTKNWRNILCRCEKCLEMYKQRKVSY 364

Query: 364 LLDEEDSIAEYEKIAKQKREEKLQQQEGAELKMFDNLGHVEKIEILSGIADMKDEIRTFL 418
           LLD ED+I EYEK AK+KR EKL++QEG  L + +NL HV K+E+L GI D +D ++  +
Sbjct: 365 LLDAEDTIVEYEKKAKEKRTEKLEKQEGEALDLLNNLDHVSKVELLHGIKDFQDGLQGLM 424

BLAST of Moc06g33680 vs. TAIR 10
Match: AT4G23860.2 (PHD finger protein-related )

HSP 1 Score: 463.0 bits (1190), Expect = 2.5e-130
Identity = 239/449 (53.23%), Postives = 300/449 (66.82%), Query Frame = 0

Query: 4   VFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDG 63
           VF+DE E TI+I EY++ ++ +EL ADLVLGGDEG ECT+ KGYMKRQAIFSC+TCTP+G
Sbjct: 5   VFEDEAEGTITINEYIERLDAEELAADLVLGGDEGDECTFPKGYMKRQAIFSCITCTPEG 64

Query: 64  NAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYNHNF 123
           NAG+CTAC LSCHDGHE++ELWTKRNFRCDCGNSKFG   CKL  +K +EN+ NSYNHNF
Sbjct: 65  NAGICTACCLSCHDGHELLELWTKRNFRCDCGNSKFGTLACKLLPSKDIENSENSYNHNF 124

Query: 124 KGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSID----EIPRDEEGVPQYE 183
           KG YC C+RPYPDP+VEEQVEMIQCCLCEDWFHEEHLGL   D    +IPRDEE  P YE
Sbjct: 125 KGLYCTCDRPYPDPNVEEQVEMIQCCLCEDWFHEEHLGLTPSDSVGSQIPRDEESEPIYE 184

Query: 184 DFICKACSAVCSFLCQYPSSIWAVGR----------HPDISLDVSVKNDVPEQ------- 243
           DFIC+ CS  CSFL  YP ++W V +             I LD +  +  P Q       
Sbjct: 185 DFICQNCSPACSFLTLYPENLWVVAKVDSTGSANACSETIELDKNHMDSEPGQPENGTDA 244

Query: 244 ---TLSVCTSVKHDND-GNPDDSANADLTVAPEESAGKDSFLSELPENNS---------C 303
               +  C+    D++ G P++   A+ +V  + S   D   +  PEN++         C
Sbjct: 245 EKSVVGKCSETISDSEPGQPENGTEAEKSVVQKCSEKIDESEAGQPENSTEAEKFVVRKC 304

Query: 304 SNETI-NEVDQTASCVLGTSQSSASVVVENKPMFLSKNWRGALCRCEKCINNYKLKNISF 363
           S +   +E    A CV+ T  +S     E KP+FL+KNWR  LCRCEKC+  YK + +S+
Sbjct: 305 SEKIDGSENVPAAGCVIRTDLNSCP-EFEKKPLFLTKNWRNILCRCEKCLEMYKQRKVSY 364

Query: 364 LLDEEDSIAEYEKIAKQKREEKLQQQEGAELKMFDNLGHVEKIEILSGIADMKDEIRTFL 418
           LLD ED+I EYEK AK+KR EKL++QEG  L + +NL HV K+E+L GI D +D ++  +
Sbjct: 365 LLDAEDTIVEYEKKAKEKRTEKLEKQEGEALDLLNNLDHVSKVELLHGIKDFQDGLQGLM 424

BLAST of Moc06g33680 vs. TAIR 10
Match: AT4G23860.3 (PHD finger protein-related )

HSP 1 Score: 463.0 bits (1190), Expect = 2.5e-130
Identity = 239/449 (53.23%), Postives = 300/449 (66.82%), Query Frame = 0

Query: 4   VFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDG 63
           VF+DE E TI+I EY++ ++ +EL ADLVLGGDEG ECT+ KGYMKRQAIFSC+TCTP+G
Sbjct: 5   VFEDEAEGTITINEYIERLDAEELAADLVLGGDEGDECTFPKGYMKRQAIFSCITCTPEG 64

Query: 64  NAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYNHNF 123
           NAG+CTAC LSCHDGHE++ELWTKRNFRCDCGNSKFG   CKL  +K +EN+ NSYNHNF
Sbjct: 65  NAGICTACCLSCHDGHELLELWTKRNFRCDCGNSKFGTLACKLLPSKDIENSENSYNHNF 124

Query: 124 KGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSID----EIPRDEEGVPQYE 183
           KG YC C+RPYPDP+VEEQVEMIQCCLCEDWFHEEHLGL   D    +IPRDEE  P YE
Sbjct: 125 KGLYCTCDRPYPDPNVEEQVEMIQCCLCEDWFHEEHLGLTPSDSVGSQIPRDEESEPIYE 184

Query: 184 DFICKACSAVCSFLCQYPSSIWAVGR----------HPDISLDVSVKNDVPEQ------- 243
           DFIC+ CS  CSFL  YP ++W V +             I LD +  +  P Q       
Sbjct: 185 DFICQNCSPACSFLTLYPENLWVVAKVDSTGSANACSETIELDKNHMDSEPGQPENGTDA 244

Query: 244 ---TLSVCTSVKHDND-GNPDDSANADLTVAPEESAGKDSFLSELPENNS---------C 303
               +  C+    D++ G P++   A+ +V  + S   D   +  PEN++         C
Sbjct: 245 EKSVVGKCSETISDSEPGQPENGTEAEKSVVQKCSEKIDESEAGQPENSTEAEKFVVRKC 304

Query: 304 SNETI-NEVDQTASCVLGTSQSSASVVVENKPMFLSKNWRGALCRCEKCINNYKLKNISF 363
           S +   +E    A CV+ T  +S     E KP+FL+KNWR  LCRCEKC+  YK + +S+
Sbjct: 305 SEKIDGSENVPAAGCVIRTDLNSCP-EFEKKPLFLTKNWRNILCRCEKCLEMYKQRKVSY 364

Query: 364 LLDEEDSIAEYEKIAKQKREEKLQQQEGAELKMFDNLGHVEKIEILSGIADMKDEIRTFL 418
           LLD ED+I EYEK AK+KR EKL++QEG  L + +NL HV K+E+L GI D +D ++  +
Sbjct: 365 LLDAEDTIVEYEKKAKEKRTEKLEKQEGEALDLLNNLDHVSKVELLHGIKDFQDGLQGLM 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146039.11.2e-243100.00putative E3 ubiquitin-protein ligase UBR7 [Momordica charantia][more]
KAG6605576.14.0e-21087.08putative E3 ubiquitin-protein ligase UBR7, partial [Cucurbita argyrosperma subsp... [more]
XP_038876257.12.6e-20987.77putative E3 ubiquitin-protein ligase UBR7 [Benincasa hispida][more]
KAG7035491.11.7e-20886.36putative E3 ubiquitin-protein ligase UBR7, partial [Cucurbita argyrosperma subsp... [more]
XP_023534431.11.7e-20887.08putative E3 ubiquitin-protein ligase UBR7 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8BU041.6e-6536.94Putative E3 ubiquitin-protein ligase UBR7 OS=Mus musculus OX=10090 GN=Ubr7 PE=1 ... [more]
Q8N8062.1e-6536.15Putative E3 ubiquitin-protein ligase UBR7 OS=Homo sapiens OX=9606 GN=UBR7 PE=1 S... [more]
Q54DV01.1e-3228.17Putative E3 ubiquitin-protein ligase ubr7 OS=Dictyostelium discoideum OX=44689 G... [more]
Q093296.5e-3035.27Protein mlo2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1CWZ95.9e-244100.00putative E3 ubiquitin-protein ligase UBR7 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1H3N33.1e-20886.12putative E3 ubiquitin-protein ligase UBR7 isoform X2 OS=Cucurbita moschata OX=36... [more]
A0A6J1K3N02.6e-20786.12putative E3 ubiquitin-protein ligase UBR7 isoform X2 OS=Cucurbita maxima OX=3661... [more]
A0A6J1H2D91.7e-20685.31putative E3 ubiquitin-protein ligase UBR7 isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A6J1K5N11.4e-20585.31putative E3 ubiquitin-protein ligase UBR7 isoform X1 OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT4G23860.12.5e-13053.23PHD finger protein-related [more]
AT4G23860.22.5e-13053.23PHD finger protein-related [more]
AT4G23860.32.5e-13053.23PHD finger protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 329..349
NoneNo IPR availablePANTHERPTHR13513:SF9E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATEDcoord: 5..416
NoneNo IPR availableCDDcd15542PHD_UBR7coord: 127..186
e-value: 3.67743E-23
score: 89.7342
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 127..187
e-value: 4.2E-5
score: 33.0
IPR003126Zinc finger, UBR-typeSMARTSM00396push_1coord: 39..108
e-value: 3.0E-21
score: 86.6
IPR003126Zinc finger, UBR-typePFAMPF02207zf-UBRcoord: 41..106
e-value: 1.1E-14
score: 54.3
IPR003126Zinc finger, UBR-typePROSITEPS51157ZF_UBRcoord: 39..109
score: 9.107794
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 123..189
e-value: 1.9E-11
score: 45.5
IPR040204E3 ubiquitin-protein ligase UBR7PANTHERPTHR13513E3 UBIQUITIN-PROTEIN LIGASE UBR7coord: 5..416
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 115..199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g33680.1Moc06g33680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding