Moc06g33370 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g33370
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionethylene-responsive transcription factor CRF2-like
Locationchr6: 25320625 .. 25321593 (+)
RNA-Seq ExpressionMoc06g33370
SyntenyMoc06g33370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATGGATTTCGACTCTGATACTTTCGGCCTCCCCATCAAGTTCACCGAGCACCGGAATCTTCTGAAGCTTACTTCCCCGCCGGGAATTAGGCCGAGGGTTGTGCGGATTTCCGTAACCGACGGTGATGCTACCGATTCATCCAGCGACGACGAGGCCGAGATGTTCGGTCGCCACAGAGTCAAGAAGTTTGTCAATGAAATTACGATCGAGCCCTCCTGTTCCGGCGAAGGGAATGGGGTTTGGGGTTCGAATAGGTCGGCGAGGACCGGCCGGAGGAGGTCGCCGGGGAAATGTGGGGTTCCGTCGAGGAATCGTCGGTCGTCGTTGAAGGTTCCGGGTGGGAAGAAGTTCCGTGGGGTTAGGCAGAGGCCGTGGGGGAAATGGGCGGCGGAAATCAGAGACCCTCTCCGCCGTGTGCGGCTGTGGCTCGGAACTTACGACACGGCGGAGGAAGCCGCCATGGTGTACGACAACGCCGCGATTCAGCTCCGTGGACCCGACGCGCTTACCAATTTCACCACCGTCCCGAAATCCTTCACCGGGAAGTGCTCCGGGTACAACTCCGGCGAGGAATCGAACAACAACATTTGCTCTCCGACTTCCGTCCTCCGGTGCCCCTCTCCGCCCAACGAAGAAGCCCAATCGCAAATCCCCGGCGAGCACGACAACTCATGCGTGTCGGAAAATTTCTCGGAGTTCTCGGAGTTCTCGAGCTCCGACACCTTCATTCCCGATGACATTTTCGACTTCGAATCTTCGGTTCCGACCTTATTTGACGAGATGGGTCTCCAACTCCAAAGCAATTTACTCGGAGACGACGATTTCACCGCCGTCTTCCACAGCCCCGTGGATGAACTCGGACTCGGGTTCGGATTCGGGTTCGAGTACCCGACTCCCCCTTCCGACGACTACTTCCAAGACTTCGGCGATGTATTCGGGCCGGATCCTCTCGTCGCGCTCTGA

mRNA sequence

ATGAATATGGATTTCGACTCTGATACTTTCGGCCTCCCCATCAAGTTCACCGAGCACCGGAATCTTCTGAAGCTTACTTCCCCGCCGGGAATTAGGCCGAGGGTTGTGCGGATTTCCGTAACCGACGGTGATGCTACCGATTCATCCAGCGACGACGAGGCCGAGATGTTCGGTCGCCACAGAGTCAAGAAGTTTGTCAATGAAATTACGATCGAGCCCTCCTGTTCCGGCGAAGGGAATGGGGTTTGGGGTTCGAATAGGTCGGCGAGGACCGGCCGGAGGAGGTCGCCGGGGAAATGTGGGGTTCCGTCGAGGAATCGTCGGTCGTCGTTGAAGGTTCCGGGTGGGAAGAAGTTCCGTGGGGTTAGGCAGAGGCCGTGGGGGAAATGGGCGGCGGAAATCAGAGACCCTCTCCGCCGTGTGCGGCTGTGGCTCGGAACTTACGACACGGCGGAGGAAGCCGCCATGGTGTACGACAACGCCGCGATTCAGCTCCGTGGACCCGACGCGCTTACCAATTTCACCACCGTCCCGAAATCCTTCACCGGGAAGTGCTCCGGGTACAACTCCGGCGAGGAATCGAACAACAACATTTGCTCTCCGACTTCCGTCCTCCGGTGCCCCTCTCCGCCCAACGAAGAAGCCCAATCGCAAATCCCCGGCGAGCACGACAACTCATGCGTGTCGGAAAATTTCTCGGAGTTCTCGGAGTTCTCGAGCTCCGACACCTTCATTCCCGATGACATTTTCGACTTCGAATCTTCGGTTCCGACCTTATTTGACGAGATGGGTCTCCAACTCCAAAGCAATTTACTCGGAGACGACGATTTCACCGCCGTCTTCCACAGCCCCGTGGATGAACTCGGACTCGGGTTCGGATTCGGGTTCGAGTACCCGACTCCCCCTTCCGACGACTACTTCCAAGACTTCGGCGATGTATTCGGGCCGGATCCTCTCGTCGCGCTCTGA

Coding sequence (CDS)

ATGAATATGGATTTCGACTCTGATACTTTCGGCCTCCCCATCAAGTTCACCGAGCACCGGAATCTTCTGAAGCTTACTTCCCCGCCGGGAATTAGGCCGAGGGTTGTGCGGATTTCCGTAACCGACGGTGATGCTACCGATTCATCCAGCGACGACGAGGCCGAGATGTTCGGTCGCCACAGAGTCAAGAAGTTTGTCAATGAAATTACGATCGAGCCCTCCTGTTCCGGCGAAGGGAATGGGGTTTGGGGTTCGAATAGGTCGGCGAGGACCGGCCGGAGGAGGTCGCCGGGGAAATGTGGGGTTCCGTCGAGGAATCGTCGGTCGTCGTTGAAGGTTCCGGGTGGGAAGAAGTTCCGTGGGGTTAGGCAGAGGCCGTGGGGGAAATGGGCGGCGGAAATCAGAGACCCTCTCCGCCGTGTGCGGCTGTGGCTCGGAACTTACGACACGGCGGAGGAAGCCGCCATGGTGTACGACAACGCCGCGATTCAGCTCCGTGGACCCGACGCGCTTACCAATTTCACCACCGTCCCGAAATCCTTCACCGGGAAGTGCTCCGGGTACAACTCCGGCGAGGAATCGAACAACAACATTTGCTCTCCGACTTCCGTCCTCCGGTGCCCCTCTCCGCCCAACGAAGAAGCCCAATCGCAAATCCCCGGCGAGCACGACAACTCATGCGTGTCGGAAAATTTCTCGGAGTTCTCGGAGTTCTCGAGCTCCGACACCTTCATTCCCGATGACATTTTCGACTTCGAATCTTCGGTTCCGACCTTATTTGACGAGATGGGTCTCCAACTCCAAAGCAATTTACTCGGAGACGACGATTTCACCGCCGTCTTCCACAGCCCCGTGGATGAACTCGGACTCGGGTTCGGATTCGGGTTCGAGTACCCGACTCCCCCTTCCGACGACTACTTCCAAGACTTCGGCGATGTATTCGGGCCGGATCCTCTCGTCGCGCTCTGA

Protein sequence

MNMDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRHRVKKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVPKSFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENFSEFSEFSSSDTFIPDDIFDFESSVPTLFDEMGLQLQSNLLGDDDFTAVFHSPVDELGLGFGFGFEYPTPPSDDYFQDFGDVFGPDPLVAL
Homology
BLAST of Moc06g33370 vs. NCBI nr
Match: XP_038875480.1 (ethylene-responsive transcription factor CRF2-like [Benincasa hispida])

HSP 1 Score: 514.6 bits (1324), Expect = 6.1e-142
Identity = 270/326 (82.82%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 1   MNMDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRH 60
           MNMD DS  F  PIK+TEH N +KLTS P IRPRVVRISVTDGDATDSSSDDE+EMFGRH
Sbjct: 1   MNMDLDS-VFPFPIKYTEHLNHVKLTSMPEIRPRVVRISVTDGDATDSSSDDESEMFGRH 60

Query: 61  RVKKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFR 120
           RVKKFVNEITIEPSCSGEGNG+WG NRSART  RRS GKCG PSRNRRS+   P GKKFR
Sbjct: 61  RVKKFVNEITIEPSCSGEGNGIWGLNRSARTRLRRSTGKCGAPSRNRRSAKGTP-GKKFR 120

Query: 121 GVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPK 180
           GVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT  PK
Sbjct: 121 GVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQQPK 180

Query: 181 SFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEF 240
           SF GKCSGYNSGEES NNICSPTSVLRCPSPP EEAQSQIPGE DNSCVSENF SEFS+F
Sbjct: 181 SFDGKCSGYNSGEES-NNICSPTSVLRCPSPPIEEAQSQIPGEQDNSCVSENFSSEFSDF 240

Query: 241 SS-SDTFIPDDIFDFESSVPTLFDEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFGF 300
           SS SDTFIPDDIF FE+S+PTLFD+MGLQ Q+N   +D  F  VFHSPV+E+    GFGF
Sbjct: 241 SSCSDTFIPDDIFAFETSIPTLFDDMGLQNQTNFFSNDGFFNGVFHSPVEEI----GFGF 300

Query: 301 EYPTPPSDDYFQDFGDVFGPDPLVAL 323
           EYPTPP DD+FQDF DVFG DPLVAL
Sbjct: 301 EYPTPPPDDFFQDFSDVFGSDPLVAL 319

BLAST of Moc06g33370 vs. NCBI nr
Match: XP_004143957.1 (ethylene-responsive transcription factor CRF2 [Cucumis sativus] >KGN50037.1 hypothetical protein Csa_000625 [Cucumis sativus])

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-140
Identity = 266/324 (82.10%), Postives = 285/324 (87.96%), Query Frame = 0

Query: 3   MDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRHRV 62
           MD DS  F  PIK+TEH N +KLTS P IRPRVVRISVTDGDATDSSSDDE+EMFGRHRV
Sbjct: 1   MDLDS-VFSFPIKYTEHLNHVKLTSMPDIRPRVVRISVTDGDATDSSSDDESEMFGRHRV 60

Query: 63  KKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGV 122
           KKFVNEI+IEPSCSGEGN +WGSNRSARTG+RRS GKCGVPSRNRRS+ KV  GKKFRGV
Sbjct: 61  KKFVNEISIEPSCSGEGNRIWGSNRSARTGQRRSTGKCGVPSRNRRSA-KVSTGKKFRGV 120

Query: 123 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPKSF 182
           RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT   KSF
Sbjct: 121 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQQSKSF 180

Query: 183 TGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEFSS 242
             KCSGYNSGEESNNNICSPTSVLRCPSPP EEAQSQIP E DNSCVSENF SEFS+FSS
Sbjct: 181 EEKCSGYNSGEESNNNICSPTSVLRCPSPPIEEAQSQIPSELDNSCVSENFSSEFSDFSS 240

Query: 243 -SDTFIPDDIFDFESSVPTLFDEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFGFEY 302
            SDTFIPDDIF FE+S+PTLFDEMGLQ Q+N L +D  F +VFHSP++E+    GF F+Y
Sbjct: 241 CSDTFIPDDIFAFETSIPTLFDEMGLQNQTNFLSNDSFFNSVFHSPIEEI----GFRFDY 300

Query: 303 PTPPSDDYFQDFGDVFGPDPLVAL 323
           P+PP DD+FQDF DVFG DPLVAL
Sbjct: 301 PSPPPDDFFQDFSDVFGSDPLVAL 318

BLAST of Moc06g33370 vs. NCBI nr
Match: XP_008437369.1 (PREDICTED: ethylene-responsive transcription factor CRF2-like [Cucumis melo] >KAA0042699.1 ethylene-responsive transcription factor CRF2-like [Cucumis melo var. makuwa] >TYK06103.1 ethylene-responsive transcription factor CRF2-like [Cucumis melo var. makuwa])

HSP 1 Score: 507.3 bits (1305), Expect = 9.7e-140
Identity = 267/324 (82.41%), Postives = 282/324 (87.04%), Query Frame = 0

Query: 3   MDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRHRV 62
           MD DS  F  PIK+TEH N +KLTS P IRPRVVRISVTDGDATDSSSDDE+EMFGRHRV
Sbjct: 1   MDLDS-VFSFPIKYTEHLNHVKLTSMPDIRPRVVRISVTDGDATDSSSDDESEMFGRHRV 60

Query: 63  KKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGV 122
           KKFVNEI+IEPSCSGEGN +WG NRSARTG RRS GKCGVPSRNRRS+ KV  GKKFRGV
Sbjct: 61  KKFVNEISIEPSCSGEGNRIWGLNRSARTGLRRSTGKCGVPSRNRRST-KVSTGKKFRGV 120

Query: 123 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPKSF 182
           RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT   KSF
Sbjct: 121 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQQTKSF 180

Query: 183 TGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEFSS 242
           T KCSGYNSGEESNNNICSPTSVLRCPSPP EEAQSQIP E DNSCVSENF SEFS+FSS
Sbjct: 181 TEKCSGYNSGEESNNNICSPTSVLRCPSPPIEEAQSQIPSEPDNSCVSENFSSEFSDFSS 240

Query: 243 -SDTFIPDDIFDFESSVPTLFDEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFGFEY 302
            SDTFIPDDIF FE+S+PTLFDEMGLQ Q+N   +D  F  VFHSPV+E+    GF FE+
Sbjct: 241 CSDTFIPDDIFAFETSIPTLFDEMGLQNQTNFFSNDSFFNGVFHSPVEEI----GFRFEH 300

Query: 303 PTPPSDDYFQDFGDVFGPDPLVAL 323
           PT PSDD+FQDF DVFG DPLVAL
Sbjct: 301 PTSPSDDFFQDFSDVFGSDPLVAL 318

BLAST of Moc06g33370 vs. NCBI nr
Match: XP_023534143.1 (ethylene-responsive transcription factor CRF2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 506.1 bits (1302), Expect = 2.2e-139
Identity = 266/327 (81.35%), Postives = 285/327 (87.16%), Query Frame = 0

Query: 1   MNMDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRH 60
           M+MDFDS  F LP+KFTEHRN +KL+S P I PRVVRIS TDGDATDSSSDDE EMFGR 
Sbjct: 1   MSMDFDS-VFDLPVKFTEHRNHVKLSSMPEITPRVVRISFTDGDATDSSSDDENEMFGRR 60

Query: 61  RVKKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFR 120
           RVKKFVNEITIEPSCSGEGNG+WG NRSAR GRRRS GKCGVPSR+RR S+K+P GKKFR
Sbjct: 61  RVKKFVNEITIEPSCSGEGNGIWGVNRSARNGRRRSSGKCGVPSRSRR-SVKMPDGKKFR 120

Query: 121 GVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPK 180
           GVRQRPWGKWAAEIRDPLRRVR+WLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT  PK
Sbjct: 121 GVRQRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQSPK 180

Query: 181 SFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEF 240
               KCS Y+SGEESNNNICSPTSVLRCPSPPNEEAQSQ+PGE DNS VSENF SEFS+F
Sbjct: 181 ----KCSAYDSGEESNNNICSPTSVLRCPSPPNEEAQSQLPGEPDNSSVSENFSSEFSDF 240

Query: 241 SS-SDTFIPDDIFDFESSVPTLF-DEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFG 300
           SS SDTFIPDDIF FESS+PTL  DE+GL  Q+N  GDD  F  VFHSP++E   GFGFG
Sbjct: 241 SSYSDTFIPDDIFAFESSIPTLLDDEIGLHHQTNFFGDDSFFNGVFHSPIEE--TGFGFG 300

Query: 301 FEYPTPPSDDYFQDFGDVFGPDPLVAL 323
           FEYPTPP+DD+FQDF DVFG DPLVAL
Sbjct: 301 FEYPTPPTDDFFQDFSDVFGSDPLVAL 319

BLAST of Moc06g33370 vs. NCBI nr
Match: XP_022958372.1 (ethylene-responsive transcription factor CRF2-like [Cucurbita moschata])

HSP 1 Score: 505.0 bits (1299), Expect = 4.8e-139
Identity = 266/327 (81.35%), Postives = 284/327 (86.85%), Query Frame = 0

Query: 1   MNMDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRH 60
           MNMDFDS  F LP+KFTEHRN +KL+S P I PRVVRIS TDGDATDSSSDDE EMFGR 
Sbjct: 1   MNMDFDS-VFDLPVKFTEHRNHVKLSSMPEITPRVVRISFTDGDATDSSSDDENEMFGRR 60

Query: 61  RVKKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFR 120
           RVKKFVNEITIEPSCSGEGNG+WG NRSAR GRRRS GKCGV SR+RR S+K+P GKKFR
Sbjct: 61  RVKKFVNEITIEPSCSGEGNGIWGVNRSARNGRRRSSGKCGVSSRSRR-SVKMPDGKKFR 120

Query: 121 GVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPK 180
           GVRQRPWGKWAAEIRDPLRRVR+WLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT  PK
Sbjct: 121 GVRQRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQSPK 180

Query: 181 SFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEF 240
               KCS Y+SGEESNNNICSPTSVLRCPSPPNEEAQSQ+PGE DNS VSENF SEFS+F
Sbjct: 181 ----KCSAYDSGEESNNNICSPTSVLRCPSPPNEEAQSQLPGEPDNSSVSENFSSEFSDF 240

Query: 241 SS-SDTFIPDDIFDFESSVPTLF-DEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFG 300
           SS SDTFIPDDIF FESS+PTL  DE+GL  Q+N  GDD  F  VFHSP++E   GFGFG
Sbjct: 241 SSYSDTFIPDDIFAFESSIPTLLDDEIGLHHQTNFFGDDSFFNGVFHSPIEE--TGFGFG 300

Query: 301 FEYPTPPSDDYFQDFGDVFGPDPLVAL 323
           FEYPTPP+DD+FQDF DVFG DPLVAL
Sbjct: 301 FEYPTPPTDDFFQDFSDVFGSDPLVAL 319

BLAST of Moc06g33370 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 9.1e-56
Identity = 155/350 (44.29%), Postives = 195/350 (55.71%), Query Frame = 0

Query: 14  IKFTEHRN-----LLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAE-----MFGRHRVK 73
           IKFTEH+      + +LT+    + R++RISVTD DATDSSSDDE E     +  R RVK
Sbjct: 12  IKFTEHKTNTTTIVSELTNTH--QTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVK 71

Query: 74  KFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVR 133
           KFVNE+ ++      G+   G   S +  +R    +  V S    ++    G KKFRGVR
Sbjct: 72  KFVNEVYLDSGAVVTGS--CGQMESKKRQKRAVKSESTV-SPVVSATTTTTGEKKFRGVR 131

Query: 134 QRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVPKSFTG 193
           QRPWGKWAAEIRDPL+RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+  P + T 
Sbjct: 132 QRPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATE 191

Query: 194 K-----------------------CSGYNSGEESNNNICSPTSVLRCPSPPNE------- 253
           K                        S   S   SN+ +CSP SVLR P   +E       
Sbjct: 192 KKAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSS 251

Query: 254 -EAQSQIPGEHDNSCVSENFSEFSEFSSSDTFIPDDIFDFESSVPTLFDEMGLQLQSNLL 313
             A   +  E   + VSE FS+F    S+  F  DD+FDF SSV   +      L  +L 
Sbjct: 252 PVAAVVVKEEPSMTTVSETFSDF----SAPLFSDDDVFDFRSSVVPDY------LGGDLF 311

Query: 314 GDDDFTAVFHSPVDELGLGFGFGFEYPTPPSDDYFQDFGDVFGPDPLVAL 323
           G+D FTA       ++  GF FG    +   +D+FQD GD+FG DPL+A+
Sbjct: 312 GEDLFTA---DMCTDMNFGFDFGSGLSSWHMEDHFQDIGDLFGSDPLLAV 343

BLAST of Moc06g33370 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.4e-40
Identity = 118/319 (36.99%), Postives = 173/319 (54.23%), Query Frame = 0

Query: 13  PIKFTEHRNLLK-------LTSPPGIR--PRVVRISVTDGDATDSSSDDEAEMFGRHRVK 72
           P+K+TEH+ +++       +     +R   R+VR+S+TD DATDSSSD+E  +F R RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 73  KFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVR 132
           + +NEI +EPS S  G+     + S    R+R      V S  ++ S+     KK+RGVR
Sbjct: 71  RLINEIRVEPSSSSTGD----VSASPTKDRKR----INVDSTVQKPSVSGQNQKKYRGVR 130

Query: 133 QRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVP----- 192
           QRPWGKWAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNFT  P     
Sbjct: 131 QRPWGKWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPV 190

Query: 193 -----KSFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPG-------EHDNS 252
                +  +      +   + + ++ SPTSVL   +  +EE    +         E +  
Sbjct: 191 QEQEQEPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQE 250

Query: 253 CVSENFSEFSEFSSSDTFIPDDIF---DFESSVP--TLFDEMGLQLQSN--------LLG 293
            +S +  E +  ++ D+F  D  F    F  S+P  ++FD+    +Q          +L 
Sbjct: 251 PISWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIFDQPMSPIQPTENDFFNDLMLF 310

BLAST of Moc06g33370 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 7.7e-39
Identity = 104/219 (47.49%), Postives = 135/219 (61.64%), Query Frame = 0

Query: 13  PIKFTEHR-NLLKLTSPP------GIRPRVVRISVTDGDATDSSSDDEAE--MFGRHRVK 72
           P+K+TEH+ ++ K T         G   + V I  TD DATDSSSD++ E  +F R RVK
Sbjct: 9   PLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRRVK 68

Query: 73  KFVNEITIEPSCSGEGNGVWGSNR---SARTGRRRSPGKCGVPSRNR-RSSLKVPGG-KK 132
           +FVNEIT+EPSC+    GV   +R   S+ +   +SP      SR R  + + V G  KK
Sbjct: 69  RFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPAS----SRQRPNNKVSVSGQIKK 128

Query: 133 FRGVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVP 192
           FRGVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+  P
Sbjct: 129 FRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPP 188

Query: 193 KSFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQS 218
           +            EE    I     ++  P+P    ++S
Sbjct: 189 QE-------EEEEEEPEPVIEEKPVIMTTPTPTTSSSES 216

BLAST of Moc06g33370 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.3e-38
Identity = 120/305 (39.34%), Postives = 161/305 (52.79%), Query Frame = 0

Query: 33  PRVVRISVTDGDATDSSSDDEAEM------FGRHRVKKFVNEITIEPSCSGEGNGVWGSN 92
           PR++RISVTD  ATDSSSD+E E+        R RVKK+V E+ ++            S+
Sbjct: 10  PRILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVV---------SD 69

Query: 93  RSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVRQRPWGKWAAEIRDPLRRVRLWLG 152
           +     ++R      VP       +     +KFRGVRQRPWGKWAAEIRDP RRVR+WLG
Sbjct: 70  KEKPMKKKRKKRVVTVP------VVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLG 129

Query: 153 TYDTAEEAAMVYDNAAIQLRGPDALTNFTTVP-----KSFTGKCSGYNSGEESNNN-ICS 212
           T+DTAEEAA+VYDNAAIQLRGP+A  NF   P     +  + +  G +         + S
Sbjct: 130 TFDTAEEAAIVYDNAAIQLRGPNAELNFPPPPVTENVEEASTEVKGVSDFIIGGGECLRS 189

Query: 213 PTSVLRCP-SPPNEEAQSQIPGEHDNSCVSENFSEF--SEFSSSDTFIPDDIFDFESSVP 272
           P SVL  P S  +   + +  G      V +    F  S+F S+  F  DD+F F +S+ 
Sbjct: 190 PVSVLESPFSGESTAVKEEFVGVSTAEIVVKKEPSFNGSDF-SAPLFSDDDVFGFSTSMS 249

Query: 273 TLFDEMGLQLQSNLLGDDDFTAVFHSPVDELGLGFGFGFEYPTPPSDDYFQDFGDVFGPD 323
             F   G     NL  D  F +         G GFG G  + +   +D+FQD GD+FG D
Sbjct: 250 ESFG--GDLFGDNLFADMSFGS---------GFGFGSGSGFSSWHVEDHFQDIGDLFGSD 287

BLAST of Moc06g33370 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.8e-34
Identity = 117/315 (37.14%), Postives = 169/315 (53.65%), Query Frame = 0

Query: 14  IKFTEHRNLLKLTSPPGI-RPRVVRISVTDGDATDSSSDDE--AEMFGRHRVKKFVNEIT 73
           +KFTE+R +  + + P    PR+VRI+VTD  ATDSSSDD+    +    RVK++V EI 
Sbjct: 8   VKFTENRTVTNVAATPSNGSPRLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKEIR 67

Query: 74  IEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVRQRPWGKW 133
               C GE +    S+ +AR G+ +   +  V   +  +S+K    KK+RGVRQRPWGK+
Sbjct: 68  F---CQGESS----SSTAARKGKHKEE-ESVVVEDDVSTSVK---PKKYRGVRQRPWGKF 127

Query: 134 AAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVPKSF----TGKCS 193
           AAEIRDP  R R+WLGT+ TAEEAA+ YD AAI L+GP ALTNF T P           S
Sbjct: 128 AAEIRDPSSRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTVS 187

Query: 194 GYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPG-----EHDNSCVSENFSEFSEFSSS 253
             + G +S  ++ SPTSVLR     NEE + +I       E  ++ + E     +     
Sbjct: 188 ACDYGRDSRQSLHSPTSVLR--FNVNEETEHEIEAIELSPERKSTVIKEEEESSAGLVFP 247

Query: 254 DTFIPDDI-------FDFESSVPTLFDEMGLQLQSNLLGDDDFTAVFHSPVDELGLGFGF 310
           D ++  D+       +D E +   LF +   ++QS LL + + +    +  ++   G   
Sbjct: 248 DPYLLPDLSLAGECFWDTEIAPDLLFLDEETKIQSTLLPNTEVSKQGENETEDFEFGLID 307

BLAST of Moc06g33370 vs. ExPASy TrEMBL
Match: A0A0A0KNS8 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G151530 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.3e-141
Identity = 266/324 (82.10%), Postives = 285/324 (87.96%), Query Frame = 0

Query: 3   MDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRHRV 62
           MD DS  F  PIK+TEH N +KLTS P IRPRVVRISVTDGDATDSSSDDE+EMFGRHRV
Sbjct: 1   MDLDS-VFSFPIKYTEHLNHVKLTSMPDIRPRVVRISVTDGDATDSSSDDESEMFGRHRV 60

Query: 63  KKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGV 122
           KKFVNEI+IEPSCSGEGN +WGSNRSARTG+RRS GKCGVPSRNRRS+ KV  GKKFRGV
Sbjct: 61  KKFVNEISIEPSCSGEGNRIWGSNRSARTGQRRSTGKCGVPSRNRRSA-KVSTGKKFRGV 120

Query: 123 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPKSF 182
           RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT   KSF
Sbjct: 121 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQQSKSF 180

Query: 183 TGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEFSS 242
             KCSGYNSGEESNNNICSPTSVLRCPSPP EEAQSQIP E DNSCVSENF SEFS+FSS
Sbjct: 181 EEKCSGYNSGEESNNNICSPTSVLRCPSPPIEEAQSQIPSELDNSCVSENFSSEFSDFSS 240

Query: 243 -SDTFIPDDIFDFESSVPTLFDEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFGFEY 302
            SDTFIPDDIF FE+S+PTLFDEMGLQ Q+N L +D  F +VFHSP++E+    GF F+Y
Sbjct: 241 CSDTFIPDDIFAFETSIPTLFDEMGLQNQTNFLSNDSFFNSVFHSPIEEI----GFRFDY 300

Query: 303 PTPPSDDYFQDFGDVFGPDPLVAL 323
           P+PP DD+FQDF DVFG DPLVAL
Sbjct: 301 PSPPPDDFFQDFSDVFGSDPLVAL 318

BLAST of Moc06g33370 vs. ExPASy TrEMBL
Match: A0A5A7TMW3 (Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G002040 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 4.7e-140
Identity = 267/324 (82.41%), Postives = 282/324 (87.04%), Query Frame = 0

Query: 3   MDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRHRV 62
           MD DS  F  PIK+TEH N +KLTS P IRPRVVRISVTDGDATDSSSDDE+EMFGRHRV
Sbjct: 1   MDLDS-VFSFPIKYTEHLNHVKLTSMPDIRPRVVRISVTDGDATDSSSDDESEMFGRHRV 60

Query: 63  KKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGV 122
           KKFVNEI+IEPSCSGEGN +WG NRSARTG RRS GKCGVPSRNRRS+ KV  GKKFRGV
Sbjct: 61  KKFVNEISIEPSCSGEGNRIWGLNRSARTGLRRSTGKCGVPSRNRRST-KVSTGKKFRGV 120

Query: 123 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPKSF 182
           RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT   KSF
Sbjct: 121 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQQTKSF 180

Query: 183 TGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEFSS 242
           T KCSGYNSGEESNNNICSPTSVLRCPSPP EEAQSQIP E DNSCVSENF SEFS+FSS
Sbjct: 181 TEKCSGYNSGEESNNNICSPTSVLRCPSPPIEEAQSQIPSEPDNSCVSENFSSEFSDFSS 240

Query: 243 -SDTFIPDDIFDFESSVPTLFDEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFGFEY 302
            SDTFIPDDIF FE+S+PTLFDEMGLQ Q+N   +D  F  VFHSPV+E+    GF FE+
Sbjct: 241 CSDTFIPDDIFAFETSIPTLFDEMGLQNQTNFFSNDSFFNGVFHSPVEEI----GFRFEH 300

Query: 303 PTPPSDDYFQDFGDVFGPDPLVAL 323
           PT PSDD+FQDF DVFG DPLVAL
Sbjct: 301 PTSPSDDFFQDFSDVFGSDPLVAL 318

BLAST of Moc06g33370 vs. ExPASy TrEMBL
Match: A0A1S3AUG2 (ethylene-responsive transcription factor CRF2-like OS=Cucumis melo OX=3656 GN=LOC103482804 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 4.7e-140
Identity = 267/324 (82.41%), Postives = 282/324 (87.04%), Query Frame = 0

Query: 3   MDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRHRV 62
           MD DS  F  PIK+TEH N +KLTS P IRPRVVRISVTDGDATDSSSDDE+EMFGRHRV
Sbjct: 1   MDLDS-VFSFPIKYTEHLNHVKLTSMPDIRPRVVRISVTDGDATDSSSDDESEMFGRHRV 60

Query: 63  KKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGV 122
           KKFVNEI+IEPSCSGEGN +WG NRSARTG RRS GKCGVPSRNRRS+ KV  GKKFRGV
Sbjct: 61  KKFVNEISIEPSCSGEGNRIWGLNRSARTGLRRSTGKCGVPSRNRRST-KVSTGKKFRGV 120

Query: 123 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPKSF 182
           RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT   KSF
Sbjct: 121 RQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQQTKSF 180

Query: 183 TGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEFSS 242
           T KCSGYNSGEESNNNICSPTSVLRCPSPP EEAQSQIP E DNSCVSENF SEFS+FSS
Sbjct: 181 TEKCSGYNSGEESNNNICSPTSVLRCPSPPIEEAQSQIPSEPDNSCVSENFSSEFSDFSS 240

Query: 243 -SDTFIPDDIFDFESSVPTLFDEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFGFEY 302
            SDTFIPDDIF FE+S+PTLFDEMGLQ Q+N   +D  F  VFHSPV+E+    GF FE+
Sbjct: 241 CSDTFIPDDIFAFETSIPTLFDEMGLQNQTNFFSNDSFFNGVFHSPVEEI----GFRFEH 300

Query: 303 PTPPSDDYFQDFGDVFGPDPLVAL 323
           PT PSDD+FQDF DVFG DPLVAL
Sbjct: 301 PTSPSDDFFQDFSDVFGSDPLVAL 318

BLAST of Moc06g33370 vs. ExPASy TrEMBL
Match: A0A6J1H1N3 (ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662 GN=LOC111459615 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.3e-139
Identity = 266/327 (81.35%), Postives = 284/327 (86.85%), Query Frame = 0

Query: 1   MNMDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRH 60
           MNMDFDS  F LP+KFTEHRN +KL+S P I PRVVRIS TDGDATDSSSDDE EMFGR 
Sbjct: 1   MNMDFDS-VFDLPVKFTEHRNHVKLSSMPEITPRVVRISFTDGDATDSSSDDENEMFGRR 60

Query: 61  RVKKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFR 120
           RVKKFVNEITIEPSCSGEGNG+WG NRSAR GRRRS GKCGV SR+RR S+K+P GKKFR
Sbjct: 61  RVKKFVNEITIEPSCSGEGNGIWGVNRSARNGRRRSSGKCGVSSRSRR-SVKMPDGKKFR 120

Query: 121 GVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPK 180
           GVRQRPWGKWAAEIRDPLRRVR+WLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT  PK
Sbjct: 121 GVRQRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQSPK 180

Query: 181 SFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEF 240
               KCS Y+SGEESNNNICSPTSVLRCPSPPNEEAQSQ+PGE DNS VSENF SEFS+F
Sbjct: 181 ----KCSAYDSGEESNNNICSPTSVLRCPSPPNEEAQSQLPGEPDNSSVSENFSSEFSDF 240

Query: 241 SS-SDTFIPDDIFDFESSVPTLF-DEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFG 300
           SS SDTFIPDDIF FESS+PTL  DE+GL  Q+N  GDD  F  VFHSP++E   GFGFG
Sbjct: 241 SSYSDTFIPDDIFAFESSIPTLLDDEIGLHHQTNFFGDDSFFNGVFHSPIEE--TGFGFG 300

Query: 301 FEYPTPPSDDYFQDFGDVFGPDPLVAL 323
           FEYPTPP+DD+FQDF DVFG DPLVAL
Sbjct: 301 FEYPTPPTDDFFQDFSDVFGSDPLVAL 319

BLAST of Moc06g33370 vs. ExPASy TrEMBL
Match: A0A6J1JZX7 (ethylene-responsive transcription factor CRF2-like OS=Cucurbita maxima OX=3661 GN=LOC111491214 PE=4 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.1e-136
Identity = 261/327 (79.82%), Postives = 281/327 (85.93%), Query Frame = 0

Query: 1   MNMDFDSDTFGLPIKFTEHRNLLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAEMFGRH 60
           MNMDFDS  F LP+KFTEHRN +K +S P I PRVVRIS TDGDATDSSSDDE EMFGR 
Sbjct: 1   MNMDFDS-VFDLPVKFTEHRNHVKHSSMPEITPRVVRISFTDGDATDSSSDDENEMFGRR 60

Query: 61  RVKKFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFR 120
           RVKKFVNEITIEPSCSGEGNG+WG NRSAR GRRRS GKCGV SR+RR S+K+P GKKFR
Sbjct: 61  RVKKFVNEITIEPSCSGEGNGIWGVNRSARNGRRRSSGKCGVSSRSRR-SVKMPDGKKFR 120

Query: 121 GVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT-VPK 180
           GVRQRPWGKWAAEIRDPLRRVR+WLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTT  PK
Sbjct: 121 GVRQRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTQSPK 180

Query: 181 SFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPGEHDNSCVSENF-SEFSEF 240
               KCS Y+SGEESNNNICSPTSVLRCPSP NEE QSQ+PGE DNS VSENF SEFS+F
Sbjct: 181 ----KCSAYDSGEESNNNICSPTSVLRCPSPSNEEDQSQLPGEPDNSSVSENFSSEFSDF 240

Query: 241 SS-SDTFIPDDIFDFESSVPTLF-DEMGLQLQSNLLGDDD-FTAVFHSPVDELGLGFGFG 300
           SS SDTFIPDDIF FE+S+PTL  DE+GL  Q+N  GDD  F  VFHSP+++   GFGFG
Sbjct: 241 SSYSDTFIPDDIFAFETSIPTLLDDEIGLHHQTNFFGDDSFFNGVFHSPIEK--TGFGFG 300

Query: 301 FEYPTPPSDDYFQDFGDVFGPDPLVAL 323
           FEYPTPP+DD+FQDF DVFG DPLVAL
Sbjct: 301 FEYPTPPTDDFFQDFSDVFGSDPLVAL 319

BLAST of Moc06g33370 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 218.8 bits (556), Expect = 6.5e-57
Identity = 155/350 (44.29%), Postives = 195/350 (55.71%), Query Frame = 0

Query: 14  IKFTEHRN-----LLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAE-----MFGRHRVK 73
           IKFTEH+      + +LT+    + R++RISVTD DATDSSSDDE E     +  R RVK
Sbjct: 12  IKFTEHKTNTTTIVSELTNTH--QTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVK 71

Query: 74  KFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVR 133
           KFVNE+ ++      G+   G   S +  +R    +  V S    ++    G KKFRGVR
Sbjct: 72  KFVNEVYLDSGAVVTGS--CGQMESKKRQKRAVKSESTV-SPVVSATTTTTGEKKFRGVR 131

Query: 134 QRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVPKSFTG 193
           QRPWGKWAAEIRDPL+RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+  P + T 
Sbjct: 132 QRPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATE 191

Query: 194 K-----------------------CSGYNSGEESNNNICSPTSVLRCPSPPNE------- 253
           K                        S   S   SN+ +CSP SVLR P   +E       
Sbjct: 192 KKAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSS 251

Query: 254 -EAQSQIPGEHDNSCVSENFSEFSEFSSSDTFIPDDIFDFESSVPTLFDEMGLQLQSNLL 313
             A   +  E   + VSE FS+F    S+  F  DD+FDF SSV   +      L  +L 
Sbjct: 252 PVAAVVVKEEPSMTTVSETFSDF----SAPLFSDDDVFDFRSSVVPDY------LGGDLF 311

Query: 314 GDDDFTAVFHSPVDELGLGFGFGFEYPTPPSDDYFQDFGDVFGPDPLVAL 323
           G+D FTA       ++  GF FG    +   +D+FQD GD+FG DPL+A+
Sbjct: 312 GEDLFTA---DMCTDMNFGFDFGSGLSSWHMEDHFQDIGDLFGSDPLLAV 343

BLAST of Moc06g33370 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 218.8 bits (556), Expect = 6.5e-57
Identity = 155/350 (44.29%), Postives = 195/350 (55.71%), Query Frame = 0

Query: 14  IKFTEHRN-----LLKLTSPPGIRPRVVRISVTDGDATDSSSDDEAE-----MFGRHRVK 73
           IKFTEH+      + +LT+    + R++RISVTD DATDSSSDDE E     +  R RVK
Sbjct: 12  IKFTEHKTNTTTIVSELTNTH--QTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVK 71

Query: 74  KFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVR 133
           KFVNE+ ++      G+   G   S +  +R    +  V S    ++    G KKFRGVR
Sbjct: 72  KFVNEVYLDSGAVVTGS--CGQMESKKRQKRAVKSESTV-SPVVSATTTTTGEKKFRGVR 131

Query: 134 QRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVPKSFTG 193
           QRPWGKWAAEIRDPL+RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+  P + T 
Sbjct: 132 QRPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATE 191

Query: 194 K-----------------------CSGYNSGEESNNNICSPTSVLRCPSPPNE------- 253
           K                        S   S   SN+ +CSP SVLR P   +E       
Sbjct: 192 KKAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSS 251

Query: 254 -EAQSQIPGEHDNSCVSENFSEFSEFSSSDTFIPDDIFDFESSVPTLFDEMGLQLQSNLL 313
             A   +  E   + VSE FS+F    S+  F  DD+FDF SSV   +      L  +L 
Sbjct: 252 PVAAVVVKEEPSMTTVSETFSDF----SAPLFSDDDVFDFRSSVVPDY------LGGDLF 311

Query: 314 GDDDFTAVFHSPVDELGLGFGFGFEYPTPPSDDYFQDFGDVFGPDPLVAL 323
           G+D FTA       ++  GF FG    +   +D+FQD GD+FG DPL+A+
Sbjct: 312 GEDLFTA---DMCTDMNFGFDFGSGLSSWHMEDHFQDIGDLFGSDPLLAV 343

BLAST of Moc06g33370 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 167.5 bits (423), Expect = 1.7e-41
Identity = 118/319 (36.99%), Postives = 173/319 (54.23%), Query Frame = 0

Query: 13  PIKFTEHRNLLK-------LTSPPGIR--PRVVRISVTDGDATDSSSDDEAEMFGRHRVK 72
           P+K+TEH+ +++       +     +R   R+VR+S+TD DATDSSSD+E  +F R RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 73  KFVNEITIEPSCSGEGNGVWGSNRSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVR 132
           + +NEI +EPS S  G+     + S    R+R      V S  ++ S+     KK+RGVR
Sbjct: 71  RLINEIRVEPSSSSTGD----VSASPTKDRKR----INVDSTVQKPSVSGQNQKKYRGVR 130

Query: 133 QRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVP----- 192
           QRPWGKWAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNFT  P     
Sbjct: 131 QRPWGKWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPV 190

Query: 193 -----KSFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQSQIPG-------EHDNS 252
                +  +      +   + + ++ SPTSVL   +  +EE    +         E +  
Sbjct: 191 QEQEQEPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQE 250

Query: 253 CVSENFSEFSEFSSSDTFIPDDIF---DFESSVP--TLFDEMGLQLQSN--------LLG 293
            +S +  E +  ++ D+F  D  F    F  S+P  ++FD+    +Q          +L 
Sbjct: 251 PISWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIFDQPMSPIQPTENDFFNDLMLF 310

BLAST of Moc06g33370 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 162.5 bits (410), Expect = 5.5e-40
Identity = 104/219 (47.49%), Postives = 135/219 (61.64%), Query Frame = 0

Query: 13  PIKFTEHR-NLLKLTSPP------GIRPRVVRISVTDGDATDSSSDDEAE--MFGRHRVK 72
           P+K+TEH+ ++ K T         G   + V I  TD DATDSSSD++ E  +F R RVK
Sbjct: 9   PLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRRVK 68

Query: 73  KFVNEITIEPSCSGEGNGVWGSNR---SARTGRRRSPGKCGVPSRNR-RSSLKVPGG-KK 132
           +FVNEIT+EPSC+    GV   +R   S+ +   +SP      SR R  + + V G  KK
Sbjct: 69  RFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPAS----SRQRPNNKVSVSGQIKK 128

Query: 133 FRGVRQRPWGKWAAEIRDPLRRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFTTVP 192
           FRGVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+  P
Sbjct: 129 FRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPP 188

Query: 193 KSFTGKCSGYNSGEESNNNICSPTSVLRCPSPPNEEAQS 218
           +            EE    I     ++  P+P    ++S
Sbjct: 189 QE-------EEEEEEPEPVIEEKPVIMTTPTPTTSSSES 216

BLAST of Moc06g33370 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 9.4e-40
Identity = 120/305 (39.34%), Postives = 161/305 (52.79%), Query Frame = 0

Query: 33  PRVVRISVTDGDATDSSSDDEAEM------FGRHRVKKFVNEITIEPSCSGEGNGVWGSN 92
           PR++RISVTD  ATDSSSD+E E+        R RVKK+V E+ ++            S+
Sbjct: 10  PRILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVV---------SD 69

Query: 93  RSARTGRRRSPGKCGVPSRNRRSSLKVPGGKKFRGVRQRPWGKWAAEIRDPLRRVRLWLG 152
           +     ++R      VP       +     +KFRGVRQRPWGKWAAEIRDP RRVR+WLG
Sbjct: 70  KEKPMKKKRKKRVVTVP------VVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLG 129

Query: 153 TYDTAEEAAMVYDNAAIQLRGPDALTNFTTVP-----KSFTGKCSGYNSGEESNNN-ICS 212
           T+DTAEEAA+VYDNAAIQLRGP+A  NF   P     +  + +  G +         + S
Sbjct: 130 TFDTAEEAAIVYDNAAIQLRGPNAELNFPPPPVTENVEEASTEVKGVSDFIIGGGECLRS 189

Query: 213 PTSVLRCP-SPPNEEAQSQIPGEHDNSCVSENFSEF--SEFSSSDTFIPDDIFDFESSVP 272
           P SVL  P S  +   + +  G      V +    F  S+F S+  F  DD+F F +S+ 
Sbjct: 190 PVSVLESPFSGESTAVKEEFVGVSTAEIVVKKEPSFNGSDF-SAPLFSDDDVFGFSTSMS 249

Query: 273 TLFDEMGLQLQSNLLGDDDFTAVFHSPVDELGLGFGFGFEYPTPPSDDYFQDFGDVFGPD 323
             F   G     NL  D  F +         G GFG G  + +   +D+FQD GD+FG D
Sbjct: 250 ESFG--GDLFGDNLFADMSFGS---------GFGFGSGSGFSSWHVEDHFQDIGDLFGSD 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875480.16.1e-14282.82ethylene-responsive transcription factor CRF2-like [Benincasa hispida][more]
XP_004143957.11.5e-14082.10ethylene-responsive transcription factor CRF2 [Cucumis sativus] >KGN50037.1 hypo... [more]
XP_008437369.19.7e-14082.41PREDICTED: ethylene-responsive transcription factor CRF2-like [Cucumis melo] >KA... [more]
XP_023534143.12.2e-13981.35ethylene-responsive transcription factor CRF2-like [Cucurbita pepo subsp. pepo][more]
XP_022958372.14.8e-13981.35ethylene-responsive transcription factor CRF2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SUQ29.1e-5644.29Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUE32.4e-4036.99Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FK127.7e-3947.49Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825031.3e-3839.34Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M3749.8e-3437.14Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0KNS87.3e-14182.10AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G151530 PE=... [more]
A0A5A7TMW34.7e-14082.41Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa O... [more]
A0A1S3AUG24.7e-14082.41ethylene-responsive transcription factor CRF2-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1H1N32.3e-13981.35ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1JZX74.1e-13679.82ethylene-responsive transcription factor CRF2-like OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
AT4G23750.16.5e-5744.29cytokinin response factor 2 [more]
AT4G23750.26.5e-5744.29cytokinin response factor 2 [more]
AT4G27950.11.7e-4136.99cytokinin response factor 4 [more]
AT5G53290.15.5e-4047.49cytokinin response factor 3 [more]
AT4G11140.19.4e-4039.34cytokinin response factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 141..157
score: 61.5
coord: 119..130
score: 70.91
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 118..181
e-value: 8.6E-38
score: 141.5
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 118..167
e-value: 1.3E-13
score: 51.0
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 118..175
score: 23.577684
IPR001471AP2/ERF domainCDDcd00018AP2coord: 117..174
e-value: 1.73805E-33
score: 115.832
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 117..176
e-value: 9.9E-26
score: 91.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..120
NoneNo IPR availablePANTHERPTHR31194:SF152ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF5-RELATEDcoord: 11..317
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 11..317
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 118..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g33370.1Moc06g33370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity