Moc06g33130 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g33130
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionE4 SUMO-protein ligase PIAL2-like isoform X2
Locationchr6: 25048625 .. 25083023 (-)
RNA-Seq ExpressionMoc06g33130
SyntenyMoc06g33130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCACCGTCGCCGTGTGAGTTGAACTTGAATAAAATTGCTGCACTTATAGAGGGCTTAGCTTTGAACGTCAAACATATCGGCCAATTCGACCCTGGCCAATTCTACAGTATCTGCATTTCCCTTGCCAGGTGTTGATTTATTTATTTGTAATTTAGGTTTCCAAGATTTTTTCTTGTTCTCTTCTTGTTGATATTTGATTTGCATGTTTTTTTTGTTTTTTTGTTTTTTTTACTGAATCCTGTGTGAATTTTGTAAGTAGGGAGGATTTGCTACTGGTAATATCTGCCGTTTGCTTAAGTGGTTGCAGTCAAATTCTGTATTTTCTGTACTTTCGCACTGAAAACTCAGTGGTTGCAAACTTGAACTGCGCGTGAATATAACATTGATTGTTTAAGGAGTGTTAGAGGAAGACCATCTATTGGGAGTTGGCAAAACGAAGGTTCTTTGATGAGTCCGTCTATATTGATTCATAAAGGTCCAACATTAGTGTAAATAGTGGAAGATTTAAATTACGAGGTAATTTTTGAGGTGGTAGAAAAGAAGAGTGGTCTTCAGCAACGATCGGGAGTGGGATTTATTGTTTCAAGAGGGAAGTTTGGATGATAGAAATATGACAGTAATTCCTGTGGGTGAGGCTTTTGATGTTGGTTTTGGGCCAAATTTCAAATGATGAGATATGTTTGGGGGACTCATTTTTTATTATCAAATTCCGAATTGGTGGGTGTTAATGAAGAGACGAAAAACTCACTTTATAATATTCTTATAAGACTATTTGAAAGTATGGGGTTAATCTTACTCCAGTTTTAAGTGGAAAGAAAATGAAGAAAGCTAAAAAGAAAAAACCTTCAAAAGGTAACAATACAATTAGAAAATCTAGTTTCGGTGGTAGATTATAAAAAATGAATTTTGAGTAGGTTGTAGAAGTGTTTATTTCTTGCATGGCTTTTGAGATTTTCTCGAGGTTTTAGTGGGATCTAGAACACAGTGAGTCTTGCGGTCCAGATTTGGTGGTTTTTCTTATATTTTGTTGTCTCTAATATTTCGTTATTTCTTGAAGATTGCCCAGCAAGTATAGTGAAGACTTTGTTATGTGGTTGCAAGGGCAAAGGCAGATTTTTATTTTTATAGGCTAATGGGACCTCTGGGACAATGTTATAAATAAACCAGCTATCAATTTAAGGTAAAAAATAAAGTTGTTAACTAATACTAATTAAAACAACCATAACAATTATAAAGCAAATCGCAAACAACACCCTTAATCATCAATAATCCTCAAAAGATATAAGGTCGACCCCAACCAAGCATCAGGGCCTTTTAATTTTCACTACCCACCCTTGTTGCTTGATATTAATGCCTGCAAGTGCTCTTTTACCAAGAATAATATTCATACTATGACACTAGTTGCTGCTTTGAAATTATCACGAATGAGGATAGTGTTATCAAGTTCTTTATCATCTTAAGTATTAGATGTCCAATAAATGAATTAAATTTACCCAACTCATCAGCTTAAGCTTTTGGGTTGAGTGGTGGTTTGTTCATTCTTAAGATGGTATCAGAGCGCGTGGTCCTGTGATCGAACCCCTACGTAGTCGTTTCCTCCCCATTTTATATTGATTTCCACTTATTGGGTCTTGATGTTGTTTTCTAATGCCACAAGTGAGAGGGAGTATTAGATATCCAATAAATGAATTAATTACCCAATCCATCAGTTTAAGCTTTTGGGTTGAGTGGTGGTTTGTTCATTCTTAAGATTAAGCCTAAGAAATGGGGTATCATGAAGGTGATTTAGTTTGATCTTATTGCTGCCAATTTCAGGACCCTTGAGAAAATTAGATGTATGCAAAGAGAGGTGCATGAGGATAGAAGCCGTATGCGAGAGGGAGTTAGTTAGTTAGGGAGCAGAGAAATATTTTGTAATTGTATAGGGGGACCAGTGGGCATTGTTTAGTTATGCTCTAGTATAATGATATGACTTAGAAGTGTAGTTGTCTGTGCGGGTTATATTGTGTTGTGTATTTTTGGAGAGAGTAGACATCTCATATGTTTCTGTGATTTTCTGTTTTATCTAATATCATAGTATTAATATTGGTTCCAATTGTTCTATGAGCTTTGTACTTTTATTGTACTCTTATTCCTAAGTCAAATACCTCATTGAGGATTTATAAGTAATGGCGAGACAACTAAAATGGATCTCTCTTATTCTTTTACTTTGTGGCCTTTTTTTGTATACTTTTGTTATATACTGGTGTCACTTCATGTGTTTAAATATATAAGTTTTGTGTTAAATCTTTAATTTCCTGCAGTGCAATGCAAGGTTACGACTGTAAATAATTCTTTTTCTTTTTGATAATTGGGAGCCAGCGTTTTGCTCCCTATACCTAGGGGCACCCGTGCCCTACGACTATAAATATTTTTATATCATCAATTATGTAATTTTGTTGGTCTCTTATTTATAATTATTTGTTCTAGTGTGAATGTAATTGTTGTTCTTTTAAATTTCTTTCCATGTATGTGCTTTGGCTTCTGCTCACAACCATCTATTTATTGTCTTTCTTGACTTTACTAGATTAATTTTGATAATACAGATCTATTGACTTATCTATAGCAAACAACAAAGTTCCATCTCAAGCTCACAGTTTACCTGGTCTCTTGAAACAGGTAACGTCCTTTATTCTAGTTGTAAAGGAATTTATGATGTTCTAAATCAATTTCACAGTTTACTGCATGCCCTATTGGCTTCTCTGCATTACTTTGCTTCCACCGCTTCTTGAGTTCGAATCTCAAGGCCTAAAAAAGTCTATTTCTCCCCCAAGACACTAATTTTAAAGCCAAGTACTTCGTTGCCCATTTTCTATAACTTGTTGGTCCATTTGACCGGCAATTTAAGATGGTGTCACTATATGACTCCTGGCCAATATGAGGCTTTTTGGGTATATACTTAACATAATTTGAGTAATTTCTTGACCCTAGAATGTCCAATTTCTGTCAAGTTACTACCTGTTAAATAGCATTCCTCGTTTTTGTACAATCACTACCAAATGTCACTGGCCTAAATAGTTGACTGTGTCATCTTCAAATTATTTTGACATTCTTGTTTTGGTTCTATGCTATGCCCAAATGTCACTATCCTAAATAGTTGACTGTCATCTTCAAATTATTTGACATTCTTGTTTTGGTTCTCTGCTATGCCTTAGAGATCTTGGCAGGACGTTCAATAGGTTGGGTTGCACTAGCCTATTAGTTAACTGAAACTTTTGGATATGTGAGGTAAAGATGTTGAGAAAAAAGTTGTATTTGGGTTTGGAAGATGGGTTGTCATCCTGGTGTAGAAGTATTGATATTAGCCTAATGACGGCCCAGTATAGATATTATTGTTTCGTTTTCCAGCTGCTGTATCCAAAGTCTCTGTTTCTTTGAAAGATTCATTTTCAATTTGTTATCCCTTATTTAAACTACCTTGATTGCCACTGGTATTTGATTTTTCAGCAGGAAAAATGTTCTTCCAATTTGTACTGTTTTGGTTATCCCAAGTTGTTCTTCGAGTGTATTGCTATTTGAAGTGACTGTTATTGTTCCTCTATCATAGTAACCGTAGATATAGCTTTGTTGAGGTATCCAATAGGTGGAGTGCAAGGTATTCTTTAATATCCATTCGCAATCTGCCAATGTATCTTGCCACAAAATATTGCTCATTTTCTCCAAGATTTGTCCTGGCACCCAATCTGTGGTCCGGTGGAATTATTCGGTGCAATCTACTATCGATTGAGTTTCTTGCTTGAAATTTTGATATTGATTGTATAGAATTTGTTCTCAGTTCATGGGTAAGGATCTTTCTCGCATGAGCTTCTTCATTCTTTCCCAAGTTCGGATTGGCTTCTATCCATATCTTTGTCTACTTACCTCTAATTGATCCCACCAAGCGGATGTTCCTTCGTTTAACTCGAGAGCTACTAATTTAACTTTCTTGTTATTCATGTAGCTGAAAAAAATTTCAGCATTTTTCACCCAATCAAGAAAACTTTCAATCTCCATTTTACCATTGAAGCTAAGGAGGTCAATCTTCATTATGTAGCCACTAACATTCTGGAATTGTCGTTGGTCATTTCTAGATGGAAAACTCTCAGTTCTCGGAGCCTTCTTGGATGCGCAAGCTGTTGGTTTTGAAGAACTCTATTATTCTGTTCTATTACTTGTAATTCTTGGTCAATTCTTTCATTATTTTACTGCGGACCCAAATTTTCCAATATTTCGACTATTTCTCCCATCAATTGTTTTATATCACCCACATTTTGCTGGATTTCATTGATAGCTCCTTCAACCAACACAAGCGTTTGGTTGTGGTTCGTGGTGAGTGGGCGCGGTGGTTTTCTCCGCTTCCTTTTCAGCAAGAGAATAGTCTTCTAATGTGGGGGTGGGAGTTTCTTGCTTGCCATGCTTTGATCCCAAGAATCTGGGTGCTCTAATACGAAATATGGTGCAGTAGAGAATTTTAGAAACAAAAAATATTAGGATCAGATTCTATGGTCTAGTCCGTTTGCGCAGGATTTATGGGTTTGTTTCTTTAGATGCTTTGGGATTGGCTAGCAACACGGATTGCAAGGTGATGCTGGAGGAGGTGTTGTTCCCTCTGTTTCGAGATAGGGGTACCTGCCTGTTTCTTGGCTATAGCAATTGTTCCATCCTTGTGAGTGGGAGGCCTAGGTGTAAGATTCTTGCCTCGAGGGATCTAAGACAGGGAGATTCTCTATTCCTTTTCCTGTTCATTCTAGTTGTGGATATCCTTAGTAAACTCGTCTCTATTGGAGTGGAGAAAGGTGTTCTTTTGGGTTTTGGTGTGGGTTGAGAGAAGACTTGTCTCTCTTATCTTTAGTTTGCAGATGATATCATCTTCTTCTGTTATGGAAAGGAAAGTTTGTTTATTAACTTGAATCATTTACTTGCTTTCTTTGAATCCATATTGAGGCTTAGTGACTGGGCGGCTTTGGTGGGGTGTGAAGTTAGTGCTTTTCCGTCCTCCTATTTAGGGCTTCCGTTGGGTCAAAGCCTTAAAAGTGCGTCTTACTGCTGCCAATGCTAGAAAAGATGCAGAAGCGTCTATCCTTGTGGAAGAAAGCGTTTTTCTCCAAGGGTGGGAGGTTAACTTTTATTCAGTCTGTCCTTGGGAGTATCCTGACTTACTTTCTGTCCCTCTTCTAAATTCATGTGGTCGTGAGTGAGAGCTTAGAGAAGATTACGAGGGACTCTTGTGGGAAGGTGCGGAGGAGAGGGGGGGGGGGGGTGATTCTCACTTAGTTAACTGGAAAGTCATTGCAAAGCCGCTAGAAGCTGGAGGATTGGGCATTAGGAATCTGAAGCTTAAGAATGAGGCTTTCTTGGCTAAATGGTTGTGGTATTTTTTCCATGACCCTATTGTGTTGTGGTGTAGGATTATTGTAAGCAAGTACGGACCGCACCTTTTTGACGGGTCTCGGTTGGTGGCCTGAAGTTGTCCGATAGAAATCCCTGGAAGGCTATCTCTTTGTGTTTCCCCTTTTTCCAGTTCATGAGGTGTTTTTTAGGTGGGGTGGGGGTGGTGCATCTTTTGTTTTGTTGGGTCTCTGTCCTTCTTTGACAGATCGAGAGACTTTAGAGGTTTCTGCTTTGATTAATTTGCTGTTTGATGTTCCGTTTCGTTTAGGGGGAAGTATGTGCGGGTTTGGTCCCCCAGCCTTCTAAGGGCCTTTCCTATAGTTCTTGTTTCCATATTTTGAGCTCCCCTTCTAGTGCCTCGTCAGCCCCTTTGTTTTCCTCCCTCTAGAAGGTCAAGATCCCAAAGAAGATCAAGTTCTTTAGGTGGCAACCCTTACATGGTGTAGTTTTGTGAGAAGGGTTATATTTTTCTTATTCTCATTACAACCCATTTAGGGGTAAATATACAAGAAGACCTTTCAACAAATAAGGAATTAATCTAAACCTATAAGGAAAACAATAAAATTACAGAATATACAGAGACATAATGTAAATTCTAATAAGGAAAAAACTTAATTTACGGCTTGATTTTCTACACTCCCCCTCAAGCTGGATGATATATGCTTGTCATTCCAAGCTTTGAGATTAATCTACTAAGGCTGGGTCGATGCAATGCCTTAGTTAAAATGTCAGCAGCTTGTTGTTGGGAAAATACATATCTCAGATTGATAGTATTTGTCTCAGTCTTCTCGCTGATGAAGTGACGATCTATTTCGACATGTTTCATCCGATCATGATGTATTGGATTTCTTGCAATGGCTATGGCAGATTGATTGTCACACAATACCTCTATAGTACCTTCTGTCTTCACCTTGAGTTCAATGAGAAGACGTTTCAACCAAACTCCTTCACAGATCCCATGGGCTAATGATTGAAACTCAGCTTCAGCACTACTTCGAGCTACAACTTGCTGTTTCTTACTACGCCAGGTTACTAGATTCCCCCATACATAGGAACAATATCCTGATGTTGACTTACGGTCAATAGGAGATCCAGCCCAATCTGCATCCGTGTAGACTTCAAGAGCTCGGTTTGTGGTTTTTCTGAACATCAGTCCTTTTCCAGGATCGTGTTTGAGATATCTCAATATTCAATACACAGCTTCCATATGCTCCTTTGAAGGTTTGTTCAGAAATTGACTTACCACACTAACAGCAAAACTAATGTCTGGTCGCGTATGAGTTAAGTATATTAGCTTTCCAACTAGGCGTTGATATTTGCCTCGATCAACTGGTTCATCTTCAAGATTAACTCCAAGTTTTGAATTTGCATCCATAGGAGTGTCTGTTGGTTTACAACCACTCATCCCGGTCTCTATTAGGAGATCTAACGTGTACTTTCGTTGAGTGACAGAAATCCCTTTACTAGATCTTGCTACTTCCATACCTAAGAAATACCTTAGGTGTCCCAAGTTTTTGATCTCAAATTTCTTCGAGAGGAGTTGCTTTAGACGAACCATTTCTTCAGAGACATTTCCTGTAAGTATTATATCATCAACATATACTATTAATACAGTGATCTTACTTGGTGAGAAGTGCTTGATAAAGAGGGTATGATCGGATTGACACTGAGTATAGCCGTCTTGTTTCAACACATTTGTAAATTTTTCAAACCAGGCCCGAGGTGACTGTTTTAGTCCATATAGAGACTTCCTGAGTTTACATACCTTTCCCTTAGTATATCTGTCCTCAAATCCAGGAGGTATGTCCATGTACACTTTTCCTCTACTAAATCACCATTTAGGAATGCATTCTTCACATCAAGTTGAAACAATGGCCAATCTAGATTTGTTGCAATGGAGAGAAGTACCCGAATAGTGTTTAGTTTGGCAACTGGAGCAAAAGTTTCCTGATAGTCAATCCCATAAGCTTGGGTGAAGCCTTTAGCCACCAGACGAGCTTTGAATCGTTCTATACTGCCATCTGGTTTGTGTTTGGTTGAGAAAATCCATTTACATCCAACCGTGCGCTTACCTGGAGGTAAGTCTGTAAGGATCCATGTCCCATTTTTTTCCAAAGCCTGCAATTCTTCTAGAGTAGCAGCCTTCCATTCAGGTTTCTGCAAGGCTTCTTGAACTGTGTTGGGAGTTTGAACCTGATCTAGTGAAGTCACAAAAGCTCTAAAATACGGCGACAGATTAGTGTAAGACAAGTGATACCGTATGGGATGTTGGGTACATGAGCGAATACCTTTCCTGACAGCAATGGGTCTATCATCTCCTGATGTCTCGTGTGCTATTTCAGGGACATTGAGATTTTGGTTTAGATCTTGGCTTTGCTGAGCTTGTGTAGGTTGTATTTCGTTTCTCTCAGGTTGTCTTGTTCGTCGCGAATAAACTTGCAGTTCAGCCTCAGAGGGAGATACGTGAGTTGGCTCAGAATGAGGTACAGGTGAACTCTCAGAGAATGGAATTGGCTCAGAATCAGAAGAGGGTACAAGAGAACTCTCAAAGGATGGGTTTAAAGAGAAGTCAGGAATGCAATCCCAATTTTGTGATTCAAGAACAGAATTCTCCCCCTGAAGGACAAAATTGGGATAATATGGTTGATTTTCAAAGAAGGTGACATCCATGGTATGAAAGAATTGTTGGGTGGGAGGATGATAACACTTGTATCCTTTCTTATTTGGCGAGTATCCAAGAAAGATACATTTTATGGATCGTGCATCAAGCTTACTACGATGCTGAGGATATATATGAACAAACGCAGTGCACCCAAATATTTTTAATGGTAATGGAGACACAAGATGGGATGTAGGATAAATTGAGAGTAAACTTTGTAAGGGTGTGTTGAATTTGAGAATGCGGGATGGCAAACGGTTTATAAGATAGGTAGCAGTTAGGGCAACTTCACCCCAGAAAGATTTTGGAACATGGGATGTGAGCATGAGAGAACGGGCTACATCGAGAAGATGACGATTTTTGCGTTCAACAACACCATTTTGTTGAGGTGTGTCAACACAAGAGCTTTGGTGAACAATACCATGAGATTGAAGATATGGACCAAGGGTAGAGTTGAAGAAATCACGAGCATTATCAATTTTAAGTATTTTTATATTGACTTGAAACTGAGTTTGGATCATTTTATGGAAAACGGTGAATAAGTGGCTTGTTTCAGATTTTTCTTTCATAAAGAATGTCCAACTCAAACGTGTGTGATCATCAACAAAGAGAAGAAACCAACGTGCCCCATTGATATTTTTTACCCTTGAAGGTCCCCAAATATCTCCATGGATAAGGGAGAAAGGCTGAGATGGAAGATAAATGTGAGGTGAATAACAAGCACGAGTATGTTTGGAGAGTTGACAAATCTCACACTTGAAAAATTGACTCTTTTTATTGAGAAATAAAGAAGAAAACAATCGTTCAAGATAAACAAAGTTGGGATGACCAAAACGATAGTGCAACATCATGACAAGATTATCTTTATGATTTAAGGACTCAGAAACATAAGCAGACTCACGATTAGACTGAACTACAAGTTCATTGCATTTCTTTGTTGGTGGAGGACTCGTTGCTTTAAGAAGATACAATCCCGCACATAATTCAGCATTGCCAATCATCTTCCCCGATCCCAAGTCCTGAAAAATACAAGAATTTGCAAGGAATTTAGTGTCGCAGTTCAAGTCTTGATTCAACTTACTCACAGACAATAGATTATACTCTAATGTGGGCACAAACAATATTGATGCAAGAGTTATGGAGCTTGATATCTGAATACTCCCAATTCCAGTGACTTGAACAGGTGTACCATTAGCAATTCGAACAGACAGGTTACCCGTATAAATTGAAAATGATGAGAATAAGGATCTATCACCTGTCATATGATCCGCTCCTGAATCCACTATCCATTCAGATGGAAGAGTTTGAGTGTGGAGAGCACGAGAATTTTGGTTACCTTGTTGTACATTCCCATAACCACTTACTGTTGTTGGTGGAGTTGTTCTGTTCAATAGTTGTTGTAGCATGTCCAACAACTCTTTGGTATGTGGATGTTGGTTAGAAGTTTCAGAGACAGCAGCATTGCCTCGAGTTTCTTGATCTGATTTGGTAGTGTTCGGCTTCCAGTTCGCTGGTTTTCCATGTAATTTCCAACAGGTTTTTTTGAGATGTCCCACTTTATGACAATGATCACACCATGGTCGCCCTTTGCGCTGGCGGTTATCGCTATATAGATTCCCCCAAGCAGCAAGAGCGATTGGATCATTAGGATGATCTCGTTGGGAAATGAGCGCAGATCCATTTTGAGTAGGAGGATGTTCGGTTTGTCCCAACATGACTTGCTTTCGACTTTCCTCCCTACGGACTTGGAAGAAGACTTCTCGAAGACTCGGTAATGGCTTAGTACCAAGAATGCGACCACGAACTTCATCAAGTGATTTATTTAGACCCATCAAAAATTTGAAGATCCTTTTTGTCTCCACAAATTTTCGAAAGAGGATACGATCATCAGAACATTTCCATTCATGAGTTCCAAATAAATCTAGTTGTTGCCAGTAACGAGATAAATTGGAGTAGTACGATGTAACAGTTGAATCACCTTGTTTAATTTCTTGGAGAGTAGTCTCTATCTGGAATAACTCAGCAGTGTTTTCTTTATTGGAAAATGTATCCTCAGCAGCATCCCAAATTTCTTTGGCAGTAGAAAATAGGAGAAAGTTCTCACCAATTTCAGTGGTCATGGAATTTATTAACCAGCTCATGACTTGATTATTTTCAGCCCTCCATTTGCGAAATGTTGAATCATCGGGTTTGGGTGATTCCACTTTACCGATGATATAGTCTTCCCGTCCACGACCATACATGAACATCAGCACTGATTGGGACCACTGAAGGTAATTGTGGCCATTGAGTTTGTGACATGTTATCGGATTATTGTTGCTTTCGGAAAAGGACGAGACGTTAATTTGTGATTCTGAACCTGCCATTACAAAAAAAAAGGTGTAAGGAGCTGTGATTCGGAATAAAGTATGGGTAGTAGCGGCGGCAGCGAGCTCGACGGTGGCGCGGCGGCGGAGAGCTCGATGGCGGCGGCGGAAAGCTCGGCGGCAGCGGCAGACACAGCAGCAGAGAGGCGGGCTTCTGCGCGACGAGCGACGACCGGAGCTGCGACGTGCGACGACCGGAGCTGCGACGAGCGATGACTGGAGCTGCGACGTGTGACGACCGGAGCTGCGATGGGGTGACGGCGCCTGAGACGAGAGCTGCGGCTGGGATCTGCAGCGGAAGCGGCGGCTAGGGTTTTTTCAGATTGTGACGGCGCCTGAGACGAAAGCTGCGGCTGGGATCTGCAGCGGAAGCGACGGCTAGGGTTTTTTTTTCAGATTCTGGCGGCTAGGGTTTTTCAGATTCTAGGGTTTTTCTATCTCTGATACCATGTAGTTTTGTGAGAAGGGTTATATTTTTCTTATTCTCATTACAACCCATTTAGGGGTAAATATACAAGAAGACCTTTCAACAAATAAGGAATTAATCTAAACCTATAAGGAAAACAATAAAATTACAGAATATACAGAGACATAATGTAAATTCTAATAAGGAAAAAACTTAATTTACGGCTTGATTTTCTACACATGGGAAAGTCAATACTTTGTATCATATTCAGAGGTGTTCTTCTTTTATTTTGGGTCCACATACAGAGGAGCCTTGGAGGATCTAGATCACATTCTATGGTCCTATCAGTTTGTGCAAGATTTATGGGTTCGTTTCTTTGGGTGCTTCGTGGTGTCCTTGGCTCGCAATTGGTATTGTAGGGCGATGCTAAAAGAGGTGTTGTTGTTCCCTCTGTTTTGAAATAGGGGTGCTTTTTTTTGTGGCATGCTTGTTTCTTGGGTATTTTGTGAAGTATTTGGCCAGAGAGAAACAATAGAATTTTTAGAGGGTGTAGAGATCCGATGATCTTGTTTGGGATCTCATTAGGTTTAATACATCCTTGTGGGGGTTGGTCTCTAAGGTTTTTTGTAATTACCAATTAGGTGTTATTCTTTTGGATTGGAGCCCTTTTCTTTAGTCGGTATCCTCTTCTTGGGTTGTCTTTTTGTATGTTTTTGTCTATCCTTTTCATTTCTCTCAATGAAAGCTTGGTTTCTTATCTTAAAAAAGAAAAAAAAAACAAAGAACATTATTCGTCATATTTTAATCGGTAAGCAACCAGCCATAGGAAGCCTTTAACAAACAACTAACTTAACAAAACAGAACCTAACTAACTTATAACTAACCGAACTACTTTATTAACAACTAAATAAAGTAGTTAGTTACATCACATCCTATCTCTATAATATGTAGATTGGGTTATAATTTCATGTTAAAATGATTTTCTAGCAGTTATTTGTTCAGGAATGTGTATCTGGTTGCTAAATCTTTCCTATTATGAACTTGTTTAAGCATTAGTTTACATAGGATATTTATTTGTCTAGATATGTCAGAAGAAACATACTCATCAAACAAAAGCAGCAATTATGGTGCTCATGATATCTGTCAAGGTACATTTTGCTCGTTCTTTTACATTCATGTCATGTTTATTTTCATCTTTTAGGACTTGTGATCAAGTTTCTCTAATGTTCCCCTTTATTTATGATGCACATGTTTTTTTTAAATAAAAAATATTAAAGTATGTTTAATTGTTTGTGTTATTGAATCAAAATTTGTTTAGATGAGTAGTAATCTTTCAAAAATAAGTAACAACTTTAACATCACTTTCAAGGGATGGAAGTTTGACAGTCTTTAGGTGAGTATCTTACCAATGAAGTCTGTCTAGGTAGTTTAGTTGTATAGCATATAAAGTGCATTTCAATTAGTCCATCGCTTGCAAGGAAGTTTCATTCTTTTTACATCAGCTTGCAATTGCTAAAATGTTCTTTTTGGAGCCTCTTCAACTTCCTTTGGACTGCAAGTATTAAGAAGGCTAGTTTTGAGAAAGGAGTGATGTGACTGGATAAACTTGTTAATTGGCTGATTTTAATGTGGTTGGTGAGAAAATAGATAATGGTGTCATCAATATGGAAGTTAAAGAAAAGCTTTCAGTCTTCCTTGCATGAAGAATTCGGCTGGTGATTGGATTAATTCTCCGATACCAAGGAGTGTTTTGTAAATAAAAAATGATTTTAATGCATTGTTCCCTTGCATGTAGAATTTGTTAGGGTTAAACTTGTAATTGTCTTAATTTTATTTTGTTTAGTTGGTAGGGTTATTTTCTTTTTTTACCTTGTAGGTAACTAGTGTAATTCTATCTTCTTTACCTCAAGTCTAACCTATTTGCACTGGTTGGGCATCTACTTTTTGTACACTCTTTCCTTTGGGCTAAAAAAAAAAGAAAAGAAAATATAACATTTCTCGTTCTTCTTATTCTTTTTGTAATTTTGTACAAATCACTGCTTCTTCTTGGTGCTTAAACCGCAAGTTTCTTTTGTAACTACAGTCTTATGATGCTCACCTGTGATTGGATAACGGTTCTATTAGTTCTTTTTCTTTTTTTTTTTTTTGTAAGGAGGGGGCTTTCTCATCCCCTGTCCTTTAGGCAGTATACTTTTCCTTTCATTGATGTTTCTGATAAAAAAAAAAAAGAATTGTCTTTTGGTAATTGTTTTTGTTTCTTGTTCTTTTTTTTTTAATTGAACAAAGAAATAAAAACATGTTTGGTGACCTTTTCTTGTTTTTTCCACTCTAAAATTTTATATTAAAAAAAGTAAATTTTTATATGAAAATTAATTTTTTTTAATTAAATATTCGAAATTGTTACCTTATTTTATTTTTTAATTCTTATTATGCATTTTATAAGTCAACAATAATAAAATAATGCTTTTATTTATGTTTTAGAAAAAAATTGAAACGACAACAAGAAAAAAAGAAACTTAATTTTGTTGTTTTCAAAATTTCTACACAATTTTGAAAATGTTTCTTAAAAGTGAGAAATGAGAAACAAGAAACATGAACAATTAACATAAACAATGCCAAACGGGCCCCAAATAACCTTTATTGGCTTGGTTCGGTATTATAAATGTTTAGTTCAATTGAAATAATCTTATACAGACCAAAAGTGATGTAGGCCTTAGTATTAGTTAGCCGTTAGACTGTTAGTTTCATATTTAGCCGTTGGGTAGATTAGTTAGTTAGGTTGGTTATCTATAAAAGCCAACTCTTTTGTATTTGAAAGGATTCATTCAAAGATTGAATAAAAGTTTCCGCCACTGACTTTACATCAAATTGGTATCAGAGCCCTATTTGAAACCTAGGACCTGATGGCCGGGAAGAACGCGGCAACTTCCGACAGAGGACCCACAGCGGATCCTTCAACAAACCAACCTTTGTCACCTAAATCCGCCACTGAGCGTTTACTGCTAGTGGAAGATTCCTTAGGTGAGGTACGTTCCAATGTACAAACAATTCACGGGTTAATGGAGAATCTTTTCCAAACATTTGGAGAACATAGCAATCGAGCAGGAACGGCTGCGTGACGATCTAGGCGACCGCGGTGGTCATCAAGAACGTGTACACCAGCCAAGAAACCGATCGCCTTCACCAATTATTGCAATAAATGCTGACGGACGGGGTCGACGACATCACGAGCTGCAAGTGAGACTTCAAGATTCAGATTCTTCAGGCGAAGAAGAAGATTTTCTTCGTGGAAACCCTCGCTTCGACCTTTGTGACTTCAAGAATAGAGGGGGTATTCGCACAGACTGAAAATCGATTTGCCTACTTTTTGTGGGAAAATGGATGTTGAAGCATTCTTGGACTGGATCAAGAATGTCGAAAACTTCTTTGATTATACAAATACTCCGGAAGATAAGAAAGTTTGTTTGGTCGCCTTCAAACTCAAGTCTGACGCTTCGGCTTGGTGGGATCAATTGGAAATTAGCCGTCGCCGTCAAGGGAAGCGTCCTATTTGCAGCTGGCCACGAATGCTTCGTTTGATGCGAGAACGTTTTCTTCCCGCCAATTTTGAGCAGCTCTTGTACCAACAATATCAACGGTGTCGGCAAGGGTCCAAGAATATGGCTGAGTATACGGAGGAATTCCATCGATTAGGAGCCCGAACGAACTTAACCAAATCAGAGGATTATAAGATTGCCAGATTTGTTGATGGTCTTCGCGAAGATATACAAGATCAAATGGATATTCAGTCGATTGGATTCCTTACTGATGCCATCACCATGGCTACAAAGATTGAGGATAAGATTGATAAGAAACGGCTCAATAACCCTATAAGACGTACGCTCTGGGACAAACCTGTAAGTTCTAAATTCCCTTCTTTTGATGCAGGGAAAACTGGAGGAGCTTCTACATCCACAGCACCTAAAGCCATTGATGACACCGTTAAGCCTCCTACCAAATCTGCAGAGACAGCCATTAAAAAGGGCGCCAATCCGTATGCTCGGCCTACTATTGGCAAGTGTTTTAGATGTGGCCAGTGGGCCATTTATCTAACGATTGCCCTCAGCGGCGTGCCCTTGCCTTAGTGGATGAAGAAGGTCATTACGATCCTGATGAAGAGGTTATTGCCGATGATGACACGGCCTATGTTGAGCCCGATGAAGGGGATCCTGTTAACTGTGTCATCCAACGAGTCCTTACTCCAAAGGTTGATGTTATTAATCAACGCCATTCCCTTTTTCGCACTCGCTGCACAATCAATGGTAAAATCTGCAATGTTATCGTTGACAGCGGGAGTAGCGAAAACATGGTTTCCCAGAAGTTGGTCACTGCCCTTAATTTGAAAACTGATCCTCACCCACAGCCTTACAAGGTTTCGTGGATACGCAAAGGAGGCGAGGTCCAAGTTCAATCAGTTTGTACGGTCTCGCTCTCCATTGGGAACCAATATAAAGACCAAGTTATATGTGACATCTTGGATATGGATGCGTGCCACATCCTGCTAGGCCGACCCTGGCAGTATGATCTCCAAGCTATACATCGGGGCCGTGAAAACACGTATGAATTTAGTTGGATGGGAAGAAAGATTGTTCTTCTACTAACGGTCTTAGATAATAAACAGAGAAAGGATAATCCTTCTCCATCCAAACAACTTTTCTCCTTATTTCCGGGCAAATCATTCGTACAAAAGGATGAATCCCTTCTCCTTGCCATCGTTGCTAAGGGAGATTCCACCCCATCTTCTCCTCCTCCCACACACCCTGCTATTTCCCAATTACTCCAAGAATTTCATGAAATAACAGAAGAACCCATAGGACTACCACCTTTGCGCGATATTCAGCATTGTATAGACCTTATGCCCGGTTCATCTTTGCCTCATCTTCCCCACTACAGAATGAGTCCTGCTGAATATCAAATTCTGCACCAACAGATACAAGATCTTTTGGATAAAGGATATATTCGGTCAAGCATCAGTCCTTGTGCCGTTCCTATGTTGCTCGCACCAAAGAAGGACGGATCATGGCGCATGTGTGTGGACAGTTGGGCAATCAATAAGATCACAATCAAATACCGTTTTCCGATCCCTTGTTTATCTGATCAGTTAAATCAATTACATGGCAGACGTTTCTTCTCCAAAATAGATCTAAAGAGCGGTTACCACCAAATTCGAATCCGGCCTGGAGACGAGTGGAAAACAGCATTCAAGACCAATACGGGTCTTTTTGAATGGCTGGTCATGCCATTTGGACTATCCAACGCGCCAAGTACATTTATGCGCATTATGCACTAGGTTTTGCAACCTTTTCTTAACACCTTTATTGTTGTATACTTTGATGACATACTTGTTTATAGCAAAACCTGTGATGATCATATCTTGCATCTTCGCATTTTGATTGAGACATTACATAACAATAAGCTCTATGTCAACCTAGCTAAGTGCTCCTTCATGACAACTGAAATAGCATTCTTAGGTTTTTATATAAACCAATTTGGCATTTCTGTCGACCCATCTAAAATTTCTGCAATCCAAAATTGGCCTACCCCTACCTCTATTAGAGACATCCAGTGTTTCTTAGGTCTAGCGTCATTCTATAGAAAGTTTATACAACATTTTAGTACAGTGGCAGCACGACTAACGGATTGTCTAAAAAAGGAAAATTTTTTTGGGATGCACCTCAGTAACACACCTGTTCTAGGTCTTCCTGACTTTTCTCAACCGTTTGAAGTGGCCATAGATGCTTCGGGGATAGGTATAGGTGCGGTTCTTTCCCATAATGGTCACCCATTAGAATATTTTAGTGAAAAACTCATACCTCCTAGGCAAAAATGGTGCACCTACGAACAAGAACTATATGCCTTAATAAGAGCCCTTAAACAATGGGAGCACTACCTCATCGGCCGTGAATTCATTCTCTTCACCGACCACTTTTCCCTTAAGTACATTCAAACTCAGAAAACCATTAATAGGATGCATGCTCGTTGGGTTTCTTTCTTACAATAGTTTGACTTTGTCATTAAGCATAAAGCAGGGACAAGTAGCTGATGCTTTAAGCCGTAAAGCAAGCCTTCTAACTCTTTTATTCGGCCAAGTGATTGCTTTTGACAACCTCCCTACAGCATATGAGAATGACAGTGACTTCCACACCATTTGGCAACAATGCAACCAACATGTCAATTGCAATGACTTTCACCTCCTTGATGGTTACCTGTTTAAAGGAGACCGACTGTGCATTCCCCATACTTCTTTACGGGAATCCTTGATTCGGGACTTGCACAGTGGCGGCTTGGCAGGCCATCTGGGACGTGATAAAACGTTGGACATCGTGGCTGCTCGGTTCTATTGGCCTCAACTAAGGAAAGATGTGTATAACTTTGCTTCTAAATGTTTTATTTGGGAAAATTTGTCGATGGACTTTGTTCTAGGATTGCCAAAGACACAGCGAGGTTATGATTCAGTTTTAGTCGTGGTCGACCGTTTTAGCAAAATGTCTCATTTTTTACCTTGCAAAAAGACTTCCGATGCCATCTATGTTGCCAATCTTTTTTTCCGAGAGATTGTGTGACTCCATGGTATACCAAAGACCATTGTCTCTGACAGAGATGTGAAATTAAAATTCTTGAGTTATTTTTGGAAAACCTTGTGGAAGAAATTTGATACCGGGTTGAAGTACAGCACGACCAACCATCCTCAAATAGATGGCCAAACTGAAGTTACTAACCGCACACTCGGGAATCTTATACGCTGTCTTGGTGGGACAAAACCGAAGCAATGGGACCTCACACTTGCTCAAGCCGAATTCGCCTACAACCACTTGCGCAATCGTTCCACAGGGAAGTCCCCCTTTAAAGTTGTATACACTAAACTCCCTAGACTTACTGTGGACCTCGCTAATATTCCTTCTTAATATTCCTTCTAATGTTGACTTTAATCAGGAAGCTGAACACATGGCTGAAAGGATAGTAGAGTTGCATAAAGAGGTCACAGACAACTTACTACAAGCTACAGCAACTTACAAAGAACATGCAGATACATGCAGATAGTCATCGCCGAGAGAAGCACTTCAAAGTTGCGAATTTGGTGATGGTTTACCTATGGAAGTCCAGATTTCCTACCGGAACATATCATAAACTTCATAATAAGAAGATTGGACCATTTGAAATCTTACAGAAATATGGTGCTAATGCTTATAAGATCAATCTTCCAACTGATCTGCGCATAAACCCCATATTCAACGTATCCGACATCTATGAGTACCAAGCGGCAGACTCCTTCCAAATAGCTACATAAACTCGTGGTTGAGTTTTCTTTTAAGGAGGAGGGAATATGATGTAGGCTTTAGTATTAGTTAGCCATTAGACTGTTAGTTTCATATTTAGCCGTTGGGTAGATTAGTTAGTTAGGTTGGTTATCTATAAAAGCCAACTCTTTTGTATTTGAAAGGATTCATTCAAAGATTGAATAAAAGTTTCCGCTACTGACTTTACATCAAAAAGAAAAAGAAAATAAATATATTTATTGGAAACATTTGTCACGCTGATGCAGCGATGCAGAAAATGCAGAGTTTTGTTTTTTACATCTGGATTTAAAAACTTTGTTCGCATGAGGAAGTTTCCTTTTCCGTTTTGTTTGTTAACACTGTGGTTTGAGTCAGTTGGCTTCATTTTTGTTGAGTAGAAATCATCTTGGAATGTTATCTGGTTACAAATATCTCAGCTTTCTTCTATATTCTGCATAGTAGAGTGCTTGCAAGATGAGATGGTTTTCGGAAAAGGAAGCAGAGGAACTCTACAGTCTTGCTAATGAGGTTCTTTTTATTTTTCTAAAAGTAGTATTGACTTGTTCCCTTGATAGAAGTTTGTGTATTTTATGGATTCTTAATATTTTTATGTTGCCGAGATATTATTATGCTATGTAGATTGGTAGTGATTTCTTTGGAGATGTGAATACTGGACAAACCAATTCCCTTACCACGATTACTACTGTTATGGAAAGGTGTGAATTTCTTTCTTGATTTATCTGCATTCTGTTTTCTTTCATCTTCTTTCTGAAAAAAGGACAATTTTCATTGATATAATGAAAAGTTACTAAGTTCAATAAAACCTCCCCACTTATCTCACAATCCCAAAACAAAGAAACCTTTCCCAGCTGGAACTAAGGATAGAATATGAATAACAAAAGAGCTATTTTTCTAAAAATACCCAACATGAACAATTTACTTTGAAGCTTTCTAATGCCTATACCAAATCCCCGGAGACCTCATTAAATAAACCTATAATTCCTTTCCAACCAATTATATTCAATAGAAAATATTTTACAGCATTAACACACAGCTTCTCAAGTCTCCCTTCAAACGTATTCTACAAAGCAACTACTCCATATTTGCCTTTAATGAACTATGAAAAAGCCATGAGAACCCAAAACCTTGTAGCAAAGCCAAATCTTTCTGCTTACTGAACATGAAAGAAAGGATTCAGCATTCAAGTCGGACATCTTTGTCTCAAGATATGCTGGCCACATTAAACATTTAACCCTTTTTGGACAAGTTTCTTTTTAAATTGTAGAGCACAATTCTTTTTCCAAAACATTTCCATTGTAGATCAGTTTAAGATCTAAAGATTTTACCCAAAAACTAACATTAGGCTCCAAAAGCCACACCCTCTTATCAATCTCATCCTTGTACTCACTATTCTTCCAACAAGTGTATGGATTGTGAGCCCTCAGTTTATCTGTATGAGTTTGGTTTTTCTCGACTGAATGCATTATGATTGATTATTATTCTGACTAGATCCCAGTTTGGTTTCCCCATCTTTCACTCCCGTCTTTGCCAACGTTTACTAGCCTCTTTGACGTTGCACATTCCTCCCCCTTGGTACATGAGTTTGTGGCTAGATTTGTATCGGGTTTGAGTTCGACTAATTCATCTTCTGTCTTCCTTCCTTTTATTTTCTTCAATTTTATTTCACCCCTCCAATTCAATTGCCTTTTTGATGAACTTGGATAGCTTCCCACTGCCTTCGCCTCTCTTCCATAGCCTCGAGTCTCAATTTAGTCTCCTTATTGAATCTTACTACTACAAACTCACTTATCATCTCCTCTTTATTTATTATTTATTTTTTTTATAAGAAACTTAACTTTCATTATCAAAAAGGTATATACACAAAAAGGGGAGATGAGATATCCCCACTAGCCAAGAGGTTACAAAAGAGCTTCCCTGTTGGCAAAAATTTGAGAGACACCATAATTTCAAAAAGCTTTGTTACTAGATGTCCATAAAGACACAAAAATTCTAACGTTCTCCTGAAAAACTCTTCTATCTGATTCTTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAACTCTTCTATCTGATTCTTGTCTCAGGAAAGTCTTTTTGTTACTCTCAAACCAGAGTCTCCACACCAAACCAGCTATCCTATTGGACCAAAGAAGGTTTGCGCGACTTTTAGAGTCTGTCCCTTTGATGAGCTCATGTTGAAAAGTCTGAATAGATCCATCCAGCAGGCTGTCGCAAATGTGCAATTGAAAAAACAAGTACCATTGGTCTTCTTCACTATTTTTGCACAACCAACTAGGTGATAGGCACATATGGGGCATCTGAAGTCTGCAGCAAGTATTTAGCCTCTCATGAATCAGGATCCATAGACAAATTCTAACATTTTTGGAAACCTCCAGCTGCTAAATAAAACTTCCCTTGAAATGTCTAGCATTTGAGTTCTGCTGTATGAGGCTGCCCATGAGAGATTTGACCGAGAAAACACAACTACTTCTGAGAGAGAGAATCTAGTGAGTAAATTTGTCATCCCTGCCCATTATTCAACTTCCAGGTCTGATAAATTTCTTCTAAAAAGCAAATCCCAGCCTCTACTAGCTTCTGACTAGCATTTATCTACTGTAAACTGCTTTGAATTTGCCCACCTTAGTTTTATACCAAACATCACCACTGCCTTGCAACACAAATGGATACTAAACCAGGGACCCTTAGACTTTGTTGTTCCTTTAACTGCTGGCCACCATTGCATATCGATGATATTTTCTCGTGATCACCTATCTCCAAAAGAGCTTCGATTTCAGCCACAAACCTCCAGTTCCATTTTGCAGGTAAAGCACAGTTTTTACTAGCCAAGTTACCTAAACCTAATGCCCCCATAGCCTCATACTTGGCAACCATCTCCCATTGTATAGGATTCGGACCTCTGCCCTCCTTATTGGTTTTCCATAAGAAATCCTTGATCAACTTCTCCAACCTCACTATGATTTGCTTAGGGCATTTGAAAATAGAAAAGTAATACAGTGGTATACTTGAAATCACATACTTCGCGAAGGTGAGCCTTCCTTCTCTAGAAGACAAAGGTAGTTCCACTTTGCCAACTTTATTTCCACTTTATCTTGAATAGGCTGTGGCTGCCAAAAATTTCTTATTTGCCTTACCTCCGAGAGGCATGCCCAGATAGATCAAAGGCCAAGACCCTATTTCACAACTCCAAAGGTGAACGTGGAAGCCAATTCTTCTTTTGAAACATTCGATCCTGCCACTTGAGACACGGCCAAATTAATATTAAAGCCTGAAATAGCTTCAAAACAGCACACCATGTGCTTAAAGTTTTAGAGGAGATTGTTGTCGTCTTGAGAGAAAATTACAGTGTCATCCGCAAATTTGTGGTGATTAATATGAGTCCCATCCAAACCCACTGTAAAGCCTTCAAGACAGCCTTCCATCACACCTCTATCAAGCATGCTACTTAGAACATCAGCTACAAGTACAAAAAGAAAAGGTGCCAGCTGGTCACCTTGACATAACACCTTGAAGCCTTGAAGCTTTGATACGCCAGCGTGGTCTTGCATTGATCAGGATGGAGAAGGACGTATTAGAGACGCAATCTCTCCCAAAGCCTTTCCTATGGAGCACTTCTAAGAGAAAATCCCAGTTAACCATATCATAGGCTTTCTCAAAATCTAATTTGATAATCCAACATTTTTTCTTGACTTTCTATTCATTGACAAGTTCATTTGCCACAAGGATTGGTTCCATGATTTGTCTTCCATGTATGAAGGCTGATTGATTCTTGCCTGCTGTCCAATCCAAGACTCCTCTTAATCTCCTAGCAAGGACTTTTGCCACGATTTTGTAGTGGCTTGTTGCCCTTGATTTTTTGGGAATTAGGCATCTCATTCACGCATGAGTTGACAGTGCCACTTTCGAAAAAGTCTTGGAACACCCCAAGCACGTCTTCTTTAATGATGTTCCACAACTTTTTCAAAAATTCACGAGTAAACCTGTCGGGGCCAGGGGCTTTGTCAGCTCCCATGTCTTCAGTTGTTGATCTAATTTCTTCCTCTATTAAAGTGTCTTCCAACTTCTCACTAAGTTCCTGGGCAAGGACTTCCAGTTTATGTTGCCAGGGCAATAGCAATGGTCAGATCTTTGTTAGACCCCCAATCATGAAGATGTATAGTAGGAAGGGTAAAATGGGGATTTGAGGGATGTTAGGCCATAAGTATAACGGCCAAGAGAGAGAAAATTCTAGCTGTTAGTAAGGGGAGTGAGTAGGCATATAAGGAAGGGAATGTTGTGAGGGAAGGATCATCTTGTATTTCAGTACTTTTGGCTTAATAGTGAAGCTAGGAGAGGTTCCCCACCCTCTTGAATTGGTGGGTTGGCTTTTGTATCGCCTTCGGCCTTTATTTTCCTATCAAAGTAATAGAAATTGTGAGGAGATACCTTACAATCTTCTAGAATACAACTTCTTATAAAAGGAAACAAATGGATCCACAATTTCTTTTTCTTCCAGCAAACTAACCCCGTTTTTGTTTTGAATTTTGGTGGTCAAATTCTTCTTCATTTTAGCAGTCGCATATCTGTGGAAGAGCTTGGAATTTTCATCACCCTCCTTTAGCCAAGCAGCTCTGCACTTCTACCTCCACTTCCTATCTTCTTTCAGGACTAGATCCTCCAGCTGAACCTTCAAAAGAGCTCTTCTGTTAAACTAAAATTCAGATAAGCCCATCCTTTGTTCCCAACAATCAAGCTGGCATATTTCTCTAATCAAGTTGTCCTTTTGAGTTCTTATGAAACCAAAAGTAGCTTTATTCTTACAGGCTTGAATTCTTATCTATAAAAAAAATTCTTACAGGCTTGAATTCTCCTCCTTAATATTTCTCATTTTTTAGTAATATTCTTACAGGCTTTGAATCAATTCCCAAGAAATAAATGCTCTGGTAATAATTTAATAGGAAACTAAACTTAAGATAGAAATCAATAATATAGGGGTTGGATGTATTGTGTTGTCAAAGTGAAATGCAAAGGCTTGCCAAATACTAGAGAGGCTCATTTCTCCCCCAAGACTTCCAAAATATGCTTCCCACACTTTCCTAACCCATGTTATTTATAACCATTATTCGCTTACAGACTTACCATTTAATTATTTATATACTTCCACTATCATTCCCAACAGTTTCTTGAGAATGCAGGATTTGAATTGATTATTTTAGTCTTCTTACTTTCTATTTGCATGTGTACCCAGATTTTTTCCTCGAATGAAGCTGGGTCAGATTATTGCATCTGTGGAAGTTAAGGTGGTTACTTGTCTCTCTGCTGAACTTTTTCCACTTTTTTAAAAAATTCTTTTTAAATTTTAAATTTATTACCTGGATATATTCTCATGTCATTTATGTTTTCCCTGTTCTAGCCTGGATATGGAGTATTTGCCACTGATTTCAATATTTCAAAGACAACACAATATTCACAACAGGAGAAAATAGTAAGTTCCGTGTTTTTCAGAGGCTCTTGGATACCTTAATATCCCATTTGACTTAGCTTATACGATCATTATTCAATTCAAGTTAAAATATCAATGAAAATACCTGTTTCTTACCCAAAAATGTATTATGCGATCAGTTTTGCAGAGTACTAGGTTAGGTTTATATGTTTGATTATCCAGTTCAAGTTTTTCACAATTTGGAATTGGTCTTACAGATGTAATAATGTAATAATGTATTAGACTTTAATAAGATGATGACTTAGAGGTCTTAAATTTATATGTTTTGATGCTTTTGTTGTTATTAAAAACATAAGACGTTTCATTGATTTTATTAAAAGAAACAAATTCTTAGAGTTACAAACCCAAAAGAGAGTGAAAAAAAAAGAAAAGAGAAAGGTACAAGCTAACTATGCCTCGTGAAAGCACCCCGATCAGTACAAACGTCCTGGAAAGAAAAATCAACAAATAATTTAGAAAAGGAACACCATGGAGAAATTATGAATCCGGAAATATCAAACCACCCTAACCAACACCCATGCTTATTCTCATCAACCCTTAGGTTACACTTGAAGTCTTGAACCAAATCTTGGAGATGGTGGTTTTTCGATGGCATTAACCCATATCAAATGGGATCTAGGAGGCAAAGGACCATACAGGAGATGATGAAGATTGCTTCTTGAATATTTAAAGACCCACTGCAAATTAAATATTTGGAATAGACGGAACCAACATGCGATAGCAAAGGGTCAACCAAAAAACAAATGATCTGCACCTCTGCATCCTCAACACACATTAAGCAAACGGATGGCATTAGAAAGGAATTAGGAAGCTTCTTTTGCAAAACTTCAGCTGTGTTTAGGCTTCTGTTAACAAAATCCAGATTAGAAAGTTTGTTAGCTTGGGACTCTTGAACTTCCAAATTGCCGAGAACAACTTAAAAGAGAAGAGGAAGAGTGCAGTCTAGATAGACCCAATGAATAAATTATAGTAGCTTGTGATCAGTTTTATGAAGCATCTAGTTTGGTTTATGTGTTTAGCTATTCACTTCAAGCCATTAGAGTTTGTGGGATTGGCATAATGGATGTAACCATGTATTACCCTTTTGTAGGATGAAAATAGAAAAGGCTAGGGGCCAATATTGCTTGTTTATATAAATACGTGATAAATAACTCCATCAAACTTCAAAAAATTCTTATTGTTATTCCCTATAAATGTATTTAAACAAGTATTCTTCATCTCAGATTGTGTATTTGCAAATTGATCTCTCATTGCAGCTACTATTTGTTGTTCAAAAAGATAATATTGAGACGTCTGCATGTTTAATCACTCCTCCACAGGTTAAGTATGTCTAATACTCCCCCCTAAGATGGGATTGATTTCAATTATATCTATCCTATGCTATAATGAATTATTCATTTTTATATCTGTAGCTTTCTTGTCAATGGGAGGGGAGTCAACGGTAGGACAAACACCGGATACACGGTTCGTTCAGATGCACTCAGAATACGATTTGTAAGATACTTTTCTATATACATAAATTGAATCTTCCGACTTCATTCTCTTTTAAGGATACTGGACCACAGCTTCCAACAAATATAGCTTGTATGCTTAAATTAGGATCAAATCTTCTCCAAGCAGTTGGTAACTTCAATGGTAGGATCTAATTCTGATTCTAAACATCTTGGTATTTTATAGGATAAAATAGATTCTTAGTCTCTGAGTTTGATGATAGGTTTCTATTTGATCTTAAGTTTCAAAAACTAACAGTCTGGTCCTCAACTTTTAAAAAATAGTTCTAAAAGGTCCTGGTGTCAAACTTCCGTTAGTGTCACTAACAGAATACTGACGTGACATTGGTGAGTTGGATATTTATTAATTTAATATTATTTGAAATGATTACTTTTCCTTTTTTTCTCTTCTTCTTTTTTCCCTCTTTCTCTCTCTTCCTTAGTCCTTCTTCTCTCCTCCTTTTCCAATATTCTTCCCTCTCTTCATCTTCGATTCCCAACACGTGCTGTCCACTTCCTCTGCCAAAATGATATCTCTCTTCACCTATTGCCTGTTCGTTAAAATGTTTGGTGGATATGGCGATGATTCATATTCTTGCCATCGGTTCAATTCCCAAAACTTCACTCCCTCCTCCCCTGCATGTCAATAATAGCATCTTTAGGCTCACAAGGACCAGTCTTGTAGGCAAGGGTTTGAGGTCTCTTAGTCCTAACGACTTGGAGGTCTCAAGTTCAAACCTTCCGGTGAGCTTAATACCAAAAATATTTGATGTCTCTCGGGTCCAGGCCTTGAGTTATGGAATCCAGCAAGTCACGGTAAAAAGGAGTTTATTAAGAGTTATGGAGGATGGATTACAATTCATGATCTTCCACTTATGTTCTGGGATAGAGATTGCTTCGAAGCAATTGGAAAGTATTGTGGTGGTTTAGTTAAAGTTTCAACTAAAACACTGAATCTATTTGATGCCTTTGAAGCGGTAATTTGTGTGAATCAGAATCTCTGCGGATTCTTACTTGTGGTAGTAAAGATAAGTCATAAGGCATTGGGGCCATTTACAGTTCGTATTTCGGACTGTAATCCTTCAAAATATCCACATTTTCGAGACTCTTTGGTGGAATTGGGAACATCTTCGAACCCCTTGGATCGTGCCAGATTTCAAACGGTAATGGAACTTGAAAGGTCTTTTCCAAAAGGTTTTGATATTTACTGAAACAAGGGTATTTTTCTCGGATCATGAGAACGAAGCGATCGATCTTCCCAATAAAGAATGCATGCTTTCTCAACCCCTAGGCCAACTGGACATTAATGGTGAGGATTTAAACAAGGAAGCTCAAAAGTCGGCATCTCCTCGTCAGCTGAATATTAATAATGGAAGCTTGAGAGTCTTAGATTGTTTGGAAGGCCCAAATGAGCCGCCAAAGATAATTAAAGGGTCCTCTTTCCCCAAAGAAACAAAGAAGTCAAACGGCCGTGTAGCTCCTTTCCCGCTTTTTATTCAAGAAGCAGGGAGAAAAGAGTGGCCATTAATGATTCGAGAAAGGTTGTCATGGCTGAAGAAGTAAGAGTTAATTTAATCTCTGCATTTAATGATGAAAGAGTATGTCCTCCTTCAAGTGACGCTTTGGGTTCCAAAATGAATCCAAGCATTCAATTACCACCCTCTCTATCACAAGGAATTATTAATTCTACCAAAAGAACCTCCTCGGCTGCCCCTTATGAAGTGGCTCTTGATGATGACGATTCAGATCTCAACATTAGTGGTCCGGATTCATCACCAGTTGCTAATTCTGTCAATGCACCAGATGAAGTTCTATGTGAACAATCTCTAGAGGCAGATTTAAAGATATTGTTTGCAGAAGAAAAAGGAAAAGAGGAGTCGATTTTAATTCCTTCTTTCTTTAAAGAGGCAAGTATCAATTTGATTCCTATCTCTGATTCATAATGAAGATCGTGTCATGGAATATCAAAGGCTTGTGCAAGGACGAGAAGAGGCTGAAGATTAAGAATTTTGTCCAGAATCATTGCCCTGACATGGTATGCCTACAAGAAACGAAGATTCAACGGTTCGATCCGTCCATGATTAAGTCTCTATGGAGTTCTAAAGACATAGGATGGTCAACCATTGAGGAATTCTAATGATGTGGGATGAAGGCTGTATTTGTGTAAAAGAAGTCATTAAAGGGGGGCATACGTTATCAATCCTCATCTTGGCTTACTTCAGTTTATGGTCCGACAGATTACAGGGAAAGGAAGTTTTTTCTCCAGGAACTCCGTGATATTTCGACGCTATCTGAAAATTGCTGGTGCATTGGTGGCGATTTCAACGTTATCCGGTGGCCTTCTGAAAGATCATCATGTACAAGATCATCTAAAGCAATGAAGAAGTTTAATATGCTGATTCAGGAGCTGGATTTGATAGAGATTCCCTTAGTTAATGGCAAATATACGTGGTCTAAGCCTGGAGCTCAGTCCAAGCATTCTTTATTGGACAGGTTTTTGATATCCAGCGGATGGGAGAGTGCCTTTTCCAATTCCAAGGCGCCTTGTGCTGAAAGAACTACCTCAGATCACTTTCCTATCATTTTGGAGGCTGGTGATTTCCAATGGGGCCCCAGTCCCTTTCGGTTCTTTAATTCGTGGTTGTCTAACAAAGATTGTATCGGCCTTATTGAATCTAAGTTAGCTAATGATCAATCCTATGGTTGGGCAGGTTACTCTCTGAATTCTAAGCTAAGGAATCTGAAATCTGCACTTCGTAGTTGGCATTTGCAGAATGAAAAAGGCAAAAAGGAGTCAGAGACAAGAATTTTGCAGCAAATCTCATCACTGGATTCCAAAGAAGAAGTTTGTGGATTATCTAATGAGGAAGCATAAAAGAGAAACGAGCTCCAAGTGGATCTGTTGAAACTTTATGTGGATGCCGAAAGGAACTTGTTCCAAAAAAGTAAGATTAAATGGCTAAGGGAGGGCGATGAAAACTCTAGTTTCTTCCACAAGTACTTAGCAGCTCGCAAGGGAAAATCGATTATTTCTGAATCGATCAATGAGGATGGTCAGACGCTAATCAATCAGATGGATATTGAAGAAGAAATTTTCACCTTCTTCAAAAGGTTGTATACTGCAAGCAGTGGTTCCAGTGAAGGCAGTGGTATATGAGGTGTGGTATGAAAGGAATCCTAGAATTTTTGAAGAAAAGCGACAGTCTCCATCAGCTTGTTTCGACATTGCTAGATTCAAGGCCTCTCAATGGAGTTCCTTATCCCCTCTCTTTAAATCTTATTCTCCTAGTTTGATTAATTCAAATTGGGGTGTTTTTGTGGCATCTCTATAGTACTTTCTATAATACTTCTTTTATGTTTTAGTTTTTTCACTCTTTCGTGAGTTTGTATCTTTGAACAATTTTTTTCCTTCTCATTGAAATAATGAGAAGTTCGTATCTTGTTAAAAAAAAAAAAGAGGCATAGTTGCCGGTGAATATAGGGGAGCAAACCTCTAATTCCCAGTTACCAAAATAATAATAATAATAATAATAATAATAGCGTCTTTAGGAAAATATTTACCCAAAATGGTTAGAAGGTCAAAGAACTGAACGTGATTTTGCTCGGGCCATGGATGCGATGGAAGGAAATACTCTCGCTACGCTCAACAATGGAGAGGTAGCCCATGGATTACCCCAGTAATAGGTTTAGGACCTGTATTGCCACGTGAAAGAGAACCCAGAAGCCAAATCTCACATAAAATGGCTGGATGGGCAACCTCCAGGATCAGTTGTTTTCACCAGTTTTGGCAACGGAACTGCAGCGTCAAGGGAGCAAATCAAGGAAATTGGAGGTGGGTTGGTTTGGGGTAGGTACAGATTCTTGTGGGTAGTGGAAGATAAGATAGTGGACAAGGAAGACAAAGAGGGGTTAGAGGAGGTAGTGGGGAAAGAACTGATGGAGAAGATGGAGAAGATGAAGAATATGAGGATGGTGATGAAGGATTATGTGAACCAACTGGAAATTTTGGGGCACAAAGCAGTGGGTGGGTTTATTAGCCACTGTGGGTGGAATTCTGTGATGGAAGTGGCAGTGAATGGGGTGCCGGTGTTGGAGTGGCTCCAAAGTGGGGACCAGATGATCAATGTGCAATTGGCTGCCAAGATGGGCTCGGAATGTGGGTTGAGAATTGGGGGATGCGGTGAGAAGGGTCTGGAACTTGGAGGAAGGATTAGAGAGATGATGGAGAGTGAAGCTGTTAGAGCACAAGCTGCGATGAAGGGATTTGGAGTGAAGGAATATTGGAAGGAATGCTGGAGAAGGGGAGAGAAGAGGGAAGAAGGAAGAGAGAGGAAGAGAAAAGGGAAAGAAAGAGGGAAGAGAGAGAAAGGAAAAAATAATCATTTAAAAATAATATTAAATTAATAAATGTTCAACTCACCAATGCCATGTTAGCATTCCGTCAGTGACACTAACAGAAGTTTGATGTGAGGGACCTTTTAGAACTATTTTCCAAAGATCGGGGCCTTAGAAACCTATCCTCAAACGCGGGGACCAAAATTGTATTTTACCCTATTTCATAATATGTCTCTAAAAGTCCCTTTAATCTTTGTTTAGGACATTATGCTATAGCAGTTGCCATTATAGGCACTGCACCATCGCCTGATTCATCAATGCTGCAAGACCATGTACAGCCAGTTGTTTCTACTGTGGATTCAGGTATAGCTACAATTGTGAAGGGCAGGTGGAAACCTTCTTTTGTTGTTGTTCTCTCTTCTTTTTTAATTTATTTATTTATCATTATATATTTGTAATTCAGTTCAGGATGGTCTCGCTAATGGCTTAAATGCATTTTCATTTCTTTATGCCATGGTTTTGGATGTTAGTAACTAATTTTTCTTTGGGTTTCAGATTCTGACATAATCGAGGGCCCATCACGAATATCCCTTAATTGCCCAATAAGGTTATATAGACAATTTCTGCATACAATTCTGATACTTTCAGTTTCAGCCATTGCAGTGCTTAAATTTATTTGCATGCTATTTATGTATGAATTCCTCGTGATGTGACGTGTGAGATAATTAGCATAGGCTTGGACTGTTTTGCCACTGATCTGAAACAGAGGATTTTTATTGATTTTCATCGCTGTGGAAAGCATAGGATTTAACTGTTCATGGATATGCTATGTTGTCTTACATAACTATTGGAGTGATGGGGTCAGGTTCCAGTAGGATAAATGGAGAAGGGGAAGCTGAAATAGTTGTATGTAAGGTTTATAAAACAAGCATGTTTTATAATTTAGTAAAAGTGTGTGGTCTAAAATGAAAGAGTAGGAATTGTTCCTAAATGAAATAATGAAACCATGCATTAGCTAAGATGCATGATTCACGGGAGGGAATCACCTGAAACAGTTTGAATTGTAATTATTGATTCACCATAGACAGTTAGGCTTGCTGTATTACCTGATGTCGAAATGGAGAAACCCTTTAGAGATTAGCTAAATGCTCAAACCCACAAAACTGGAGTTACAGGATAACAAAACAATAAAATTTAATAAATAACTGCAACATACTCTGATGGAGATGCACAAAACGTGAAAGCAAATTCTGATTTAGAGCTTGATATTGAATAGGTAGAGCATTGAAGAAAATGGCAGATGGGCACTGGGGAATAGTCGAATAGATAGCTGTACCAGTTACATCAAATTCAAAAATATATATCAAGAGTCTAAGCATCTGAGTTATAAAACTAGAATGAAAAGGAACCATCAAAAGCTTGTGGGGTAATGTACAGCTTACTACTTCTAGGTGGTGCGACGCTTATAGCAAATTTTTTTTTTAATTACTCCCTTTGCTAGATCCTTCAAAATTGGAAGGCGTTCATGACTTAGTGACCTTGGGAAGAAGTTTCCTCTACCCTTGCTCTAGGCTGTTCTTTTTTGTGATTAATATATTTCTTAAGTTTTCCATCCCAAAAAAAAAAAAGAAAAAGAAGAAGAAGAGGAACCATCAAATCTCAAGACAAGAAAAAAAGGAACACATTGATTACACGTGGGAATGACTGAATAATATTGCTTTTCTTATGAATTACATTTTTAATTAGATTTAAGCTGAACCATACTATTGGGTGATGCATAATTCAAAATAAAAATTAAATGTATTTTATGATCTTTATTTGCTATGATATGCAATCTCGATTCTCATAAAAAACATGATCTGATTCACTATTTGATGACCCTAGATTTCAAAAATGACGTCCAGCCAAAAGAATAGATAGCATCTGAAAGTTGGTATTTTCGTTTACACCAGTATAGAGCTAAAGATAGAATTGCCTTTCAGAAAGTATAGAAAGAGACCACAGAGTCATTAAGTATGCGATTACCTAGACCTTGGTTGTGAGAATCCGTGCGTCAAAAGTGAGTGCAGTGGTTTCTTTAACAGTAAAGCATCTATTTTTACCATCGATATTGAATTTAGAAATTGCTTGCTTAATTATTTATCTCCCCCCCTCAAGCCACCCCCACCCCCACCCCCACCCAAGGAAAATTGAAATTTTACATTCGTAATTACATGGATGAGGTCAGAGAAGTTATTATCATATCCCGTGTTTGACAATGTATACAAAATTTACTTGTTACTTGAATACACACACATACATACATATATACACATGCATACATATGTATATATATATTGTCACTTCTTGCTGACACACCTATCGAACACCAGATGAAGTTTATGATCGGGTCAAGTTTCAGTTTTTATGTAACTTAATGATTGACGTGGTGATCGATTCTTCCTGTTCTCAATGTGAAACAGCTATACCCGAATCAAGGTTCCTGTGAAAGGTTGTTCTTGTAAACATCTTCAGGTAAGCGTTCCCTGCCATGGCATCTGAAAGGTGTTGATACCTTAATCTTGGCATGATCGTTCTAAATCTTTTCCATATATTTATCTTTTTCTGTCAAGAAAATTGGCAACTGTAGCATGCACCTTGGTGCCTTTACATGTGTACCCATTCTGTTTTTTTGCTAGATTGTCTTGTAGTACATGAGTTTACTGAATATTATATCTTAAAAGTTTTCTTTAGGTTTAATATCAGTGCCTTTCTGTATGTTGCAGTGCTTTGATTTTGATAACTTCATTGACATAAATTCAAGAAGACCATCCTGGCGATGCCCACATTGTAACCAGTACATTTGCTATTTGGATATTCGTGTTGATCAAAATATGTTGAAGGCAAGTGTAGTGATCAACCTTTTCCGAATATCATTTTCATCTGCAATTTTTTCTTTTCGCTTTAATTCATTCTATGAATCCATTGTATTTCCGTTAGGTCCTTAGAGAAGTGGGAGAGAACGTCACTGAAGTGATTATTTCAGCTGATGGATCATGGAAGGCCATCCTAAATGATTATGGGGATGGTCGGCCATTGGAAGATTCTCTCAAAAACCAGAATGGAGGGGCACAGCAAGAGTCTACTGCTCCTCCTGACGTGTTAGATCTTACTGAAGTTGATGATAATATGAACATCTGCAATCTTGAGACTGAAGACAGAAAACCTTGTCTTAGTAATAAAAACCAACCGGTTTCTTCGAGTTTAAATATATCATCTGGAATGAACAGGAATAGCTTAAATCAAAATTTTGCTGCTGTCTTGGAGGATGACTTTTGGTCTGGAATAGATGAGACTTTGACCTCTATTACTAGGTCAGATGCTCCATTGGGTAATAGCACGCCTGCAACTAGTTCTGCTGATCTTATGCAATCCGCTGTCTTGACCGAAGCTGTCGCACCTGTTCTTAATCATGGTGTGGGGGTTCCAGGACATGCCGCCTTTTCATCTCCTGCATTGCATGATCAAAATTTGCAGGTTCAAGCTTTGAACTCAAATGAAAATAATCAGTATGGGAGGATGACATTAATACCAAGACCTGTCAGCAGGACTCCAGTTACAGTTCAAGCCCTTCCTGCTCAGTCCCAAGGATCAGGCCAGCTGTATAGTTTAAGAACCTCAACAATTTCCTCAGCTCCACAAGTTGGACACGGTTTAAATACAATAACTCGTGATTCAGAACGGAGACTGCAATTTCCGATACATCATGGAGATCCACATCGTGCAACAAACCTAGCTCCATTTCAGCGCCCACCAACAGTGCAGGTGCTATTTTATATTGTTTCCATGATCTTCAAGTGGTCATCTATTTTAATGTTTTTATTTTCAATGGATATGACTTTTGGTGGTGTTAGTTAAATTTCATTACTTTTAGATACAGTGAGATTTGTGCCAGGTCAACATAAGTTTCGTGACACAAGTCAGGATGAATTTTTTGTTCAAACCGTAAGGTCATGACCTCAAGAATTTAAATAAAAGCAGCATATTTTCAAGCATTAAGCCTTTACCATTGGACCAGCTCCTTAGGAATTGACATTATTTGATCGATGGCCTACAGGGAGTGTTCACGTTTTTGTTTTCATTCTGAATTTATGGCCCCCCGATTTCCTGGATGATACATTGTGCAGAGTCTATCATTTTACTGTAGATGTTAACCTATAATTTATTTGCTTATACTCTCAGATCCGGGATCCTCAAGATCGTTCCTTCACTCCTGGGCTAACTGTTCAAGCATCGACTCCTTTAAGGCCATCCTTGGGTCTATTAACGGAATTCCAGAATCCACACCTTCAGCAAGCTCTCAACATGAGGATGCCCCAACTCCGGAATCAAATTCCAAACAATGTCCGGCCATCTTTAGGATCCCCAAGAACTATGAATCAAGTAGGAGGTGGTGGATATGGCGGAGCTGCTTATGCCACAGTGACATCTAGTCAATGTGCAAGAATGATGGTTACTTCCCAGCGAGCTGAGATGCTAAAACAATCTGCAGCCATGTCATTACTAAATCAAACTTCCAGATCTGCCCATTCTCTTCAAACTACTCCTGATGGGCATAGGACAGCAGCTGCTGGGGAGGTGAGAAATGTTGGAGGAATGTCTCAATCTGTTCCCACGTCTGCAGGTTTAGTAGAACCGTCATTAGAGCAGAATTGGCAGCCCACAGGTCGAATGCGTGGCAGTCTTACTGGTCGAACTTATTCTGATGCTCTTGGCCAGTTACTTATTCAGCCAACTCAATCTGTACAATCTGCTCGACCTTCATCTAATCCGACTCCTACTCCCCCCAGTACTGCATCCACACAAGCTCAAATGTTCAATGGCAGGGACACACAAGTTCCAAGGACAAGATAA

mRNA sequence

ATGGATGCACCGTCGCCGTGTGAGTTGAACTTGAATAAAATTGCTGCACTTATAGAGGGCTTAGCTTTGAACGTCAAACATATCGGCCAATTCGACCCTGGCCAATTCTACAGTATCTGCATTTCCCTTGCCAGATCTATTGACTTATCTATAGCAAACAACAAAGTTCCATCTCAAGCTCACAGTTTACCTGGTCTCTTGAAACAGATATGTCAGAAGAAACATACTCATCAAACAAAAGCAGCAATTATGGTGCTCATGATATCTGTCAAGAGTGCTTGCAAGATGAGATGGTTTTCGGAAAAGGAAGCAGAGGAACTCTACAGTCTTGCTAATGAGATTGGTAGTGATTTCTTTGGAGATGTGAATACTGGACAAACCAATTCCCTTACCACGATTACTACTGTTATGGAAAGATTTTTTCCTCGAATGAAGCTGGGTCAGATTATTGCATCTGTGGAAGTTAAGCCTGGATATGGAGTATTTGCCACTGATTTCAATATTTCAAAGACAACACAATATTCACAACAGGAGAAAATACTACTATTTGTTGTTCAAAAAGATAATATTGAGACGTCTGCATGTTTAATCACTCCTCCACAGGTTAACTTTCTTGTCAATGGGAGGGGAGTCAACGGTAGGACAAACACCGGATACACGGATACTGGACCACAGCTTCCAACAAATATAGCTTGTATGCTTAAATTAGGATCAAATCTTCTCCAAGCAGTTGGTAACTTCAATGGACATTATGCTATAGCAGTTGCCATTATAGGCACTGCACCATCGCCTGATTCATCAATGCTGCAAGACCATGTACAGCCAGTTGTTTCTACTGTGGATTCAGATTCTGACATAATCGAGGGCCCATCACGAATATCCCTTAATTGCCCAATAAGCTATACCCGAATCAAGGTTCCTGTGAAAGGTTGTTCTTGTAAACATCTTCAGTGCTTTGATTTTGATAACTTCATTGACATAAATTCAAGAAGACCATCCTGGCGATGCCCACATTGTAACCAGTACATTTGCTATTTGGATATTCGTGTTGATCAAAATATGTTGAAGGTCCTTAGAGAAGTGGGAGAGAACGTCACTGAAGTGATTATTTCAGCTGATGGATCATGGAAGGCCATCCTAAATGATTATGGGGATGGTCGGCCATTGGAAGATTCTCTCAAAAACCAGAATGGAGGGGCACAGCAAGAGTCTACTGCTCCTCCTGACGTGTTAGATCTTACTGAAGTTGATGATAATATGAACATCTGCAATCTTGAGACTGAAGACAGAAAACCTTGTCTTAGTAATAAAAACCAACCGGTTTCTTCGAGTTTAAATATATCATCTGGAATGAACAGGAATAGCTTAAATCAAAATTTTGCTGCTGTCTTGGAGGATGACTTTTGGTCTGGAATAGATGAGACTTTGACCTCTATTACTAGGTCAGATGCTCCATTGGGTAATAGCACGCCTGCAACTAGTTCTGCTGATCTTATGCAATCCGCTGTCTTGACCGAAGCTGTCGCACCTGTTCTTAATCATGGTGTGGGGGTTCCAGGACATGCCGCCTTTTCATCTCCTGCATTGCATGATCAAAATTTGCAGGTTCAAGCTTTGAACTCAAATGAAAATAATCAGTATGGGAGGATGACATTAATACCAAGACCTGTCAGCAGGACTCCAGTTACAGTTCAAGCCCTTCCTGCTCAGTCCCAAGGATCAGGCCAGCTGTATAGTTTAAGAACCTCAACAATTTCCTCAGCTCCACAAGTTGGACACGGTTTAAATACAATAACTCGTGATTCAGAACGGAGACTGCAATTTCCGATACATCATGGAGATCCACATCGTGCAACAAACCTAGCTCCATTTCAGCGCCCACCAACAGTGCAGATCCGGGATCCTCAAGATCGTTCCTTCACTCCTGGGCTAACTGTTCAAGCATCGACTCCTTTAAGGCCATCCTTGGGTCTATTAACGGAATTCCAGAATCCACACCTTCAGCAAGCTCTCAACATGAGGATGCCCCAACTCCGGAATCAAATTCCAAACAATGTCCGGCCATCTTTAGGATCCCCAAGAACTATGAATCAAGTAGGAGGTGGTGGATATGGCGGAGCTGCTTATGCCACAGTGACATCTAGTCAATGTGCAAGAATGATGGTTACTTCCCAGCGAGCTGAGATGCTAAAACAATCTGCAGCCATGTCATTACTAAATCAAACTTCCAGATCTGCCCATTCTCTTCAAACTACTCCTGATGGGCATAGGACAGCAGCTGCTGGGGAGGTGAGAAATGTTGGAGGAATGTCTCAATCTGTTCCCACGTCTGCAGGTTTAGTAGAACCGTCATTAGAGCAGAATTGGCAGCCCACAGGTCGAATGCGTGGCAGTCTTACTGGTCGAACTTATTCTGATGCTCTTGGCCAGTTACTTATTCAGCCAACTCAATCTGTACAATCTGCTCGACCTTCATCTAATCCGACTCCTACTCCCCCCAGTACTGCATCCACACAAGCTCAAATGTTCAATGGCAGGGACACACAAGTTCCAAGGACAAGATAA

Coding sequence (CDS)

ATGGATGCACCGTCGCCGTGTGAGTTGAACTTGAATAAAATTGCTGCACTTATAGAGGGCTTAGCTTTGAACGTCAAACATATCGGCCAATTCGACCCTGGCCAATTCTACAGTATCTGCATTTCCCTTGCCAGATCTATTGACTTATCTATAGCAAACAACAAAGTTCCATCTCAAGCTCACAGTTTACCTGGTCTCTTGAAACAGATATGTCAGAAGAAACATACTCATCAAACAAAAGCAGCAATTATGGTGCTCATGATATCTGTCAAGAGTGCTTGCAAGATGAGATGGTTTTCGGAAAAGGAAGCAGAGGAACTCTACAGTCTTGCTAATGAGATTGGTAGTGATTTCTTTGGAGATGTGAATACTGGACAAACCAATTCCCTTACCACGATTACTACTGTTATGGAAAGATTTTTTCCTCGAATGAAGCTGGGTCAGATTATTGCATCTGTGGAAGTTAAGCCTGGATATGGAGTATTTGCCACTGATTTCAATATTTCAAAGACAACACAATATTCACAACAGGAGAAAATACTACTATTTGTTGTTCAAAAAGATAATATTGAGACGTCTGCATGTTTAATCACTCCTCCACAGGTTAACTTTCTTGTCAATGGGAGGGGAGTCAACGGTAGGACAAACACCGGATACACGGATACTGGACCACAGCTTCCAACAAATATAGCTTGTATGCTTAAATTAGGATCAAATCTTCTCCAAGCAGTTGGTAACTTCAATGGACATTATGCTATAGCAGTTGCCATTATAGGCACTGCACCATCGCCTGATTCATCAATGCTGCAAGACCATGTACAGCCAGTTGTTTCTACTGTGGATTCAGATTCTGACATAATCGAGGGCCCATCACGAATATCCCTTAATTGCCCAATAAGCTATACCCGAATCAAGGTTCCTGTGAAAGGTTGTTCTTGTAAACATCTTCAGTGCTTTGATTTTGATAACTTCATTGACATAAATTCAAGAAGACCATCCTGGCGATGCCCACATTGTAACCAGTACATTTGCTATTTGGATATTCGTGTTGATCAAAATATGTTGAAGGTCCTTAGAGAAGTGGGAGAGAACGTCACTGAAGTGATTATTTCAGCTGATGGATCATGGAAGGCCATCCTAAATGATTATGGGGATGGTCGGCCATTGGAAGATTCTCTCAAAAACCAGAATGGAGGGGCACAGCAAGAGTCTACTGCTCCTCCTGACGTGTTAGATCTTACTGAAGTTGATGATAATATGAACATCTGCAATCTTGAGACTGAAGACAGAAAACCTTGTCTTAGTAATAAAAACCAACCGGTTTCTTCGAGTTTAAATATATCATCTGGAATGAACAGGAATAGCTTAAATCAAAATTTTGCTGCTGTCTTGGAGGATGACTTTTGGTCTGGAATAGATGAGACTTTGACCTCTATTACTAGGTCAGATGCTCCATTGGGTAATAGCACGCCTGCAACTAGTTCTGCTGATCTTATGCAATCCGCTGTCTTGACCGAAGCTGTCGCACCTGTTCTTAATCATGGTGTGGGGGTTCCAGGACATGCCGCCTTTTCATCTCCTGCATTGCATGATCAAAATTTGCAGGTTCAAGCTTTGAACTCAAATGAAAATAATCAGTATGGGAGGATGACATTAATACCAAGACCTGTCAGCAGGACTCCAGTTACAGTTCAAGCCCTTCCTGCTCAGTCCCAAGGATCAGGCCAGCTGTATAGTTTAAGAACCTCAACAATTTCCTCAGCTCCACAAGTTGGACACGGTTTAAATACAATAACTCGTGATTCAGAACGGAGACTGCAATTTCCGATACATCATGGAGATCCACATCGTGCAACAAACCTAGCTCCATTTCAGCGCCCACCAACAGTGCAGATCCGGGATCCTCAAGATCGTTCCTTCACTCCTGGGCTAACTGTTCAAGCATCGACTCCTTTAAGGCCATCCTTGGGTCTATTAACGGAATTCCAGAATCCACACCTTCAGCAAGCTCTCAACATGAGGATGCCCCAACTCCGGAATCAAATTCCAAACAATGTCCGGCCATCTTTAGGATCCCCAAGAACTATGAATCAAGTAGGAGGTGGTGGATATGGCGGAGCTGCTTATGCCACAGTGACATCTAGTCAATGTGCAAGAATGATGGTTACTTCCCAGCGAGCTGAGATGCTAAAACAATCTGCAGCCATGTCATTACTAAATCAAACTTCCAGATCTGCCCATTCTCTTCAAACTACTCCTGATGGGCATAGGACAGCAGCTGCTGGGGAGGTGAGAAATGTTGGAGGAATGTCTCAATCTGTTCCCACGTCTGCAGGTTTAGTAGAACCGTCATTAGAGCAGAATTGGCAGCCCACAGGTCGAATGCGTGGCAGTCTTACTGGTCGAACTTATTCTGATGCTCTTGGCCAGTTACTTATTCAGCCAACTCAATCTGTACAATCTGCTCGACCTTCATCTAATCCGACTCCTACTCCCCCCAGTACTGCATCCACACAAGCTCAAATGTTCAATGGCAGGGACACACAAGTTCCAAGGACAAGATAA

Protein sequence

MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQAHSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFGDVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKILLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNLLQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGIDETLTSITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQNLQVQALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGHGLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQASTPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAYATVTSSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVGGMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNPTPTPPSTASTQAQMFNGRDTQVPRTR
Homology
BLAST of Moc06g33130 vs. NCBI nr
Match: KAG6579533.1 (E4 SUMO-protein ligase PIAL2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 641/865 (74.10%), Postives = 715/865 (82.66%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMVLMI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +NSLTTITTVMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNL 240
            LFV QKDN ETSAC+I+PPQVNFLVNGRGVNGRTN  Y DTGPQLPTN+  MLKLGSNL
Sbjct: 181 RLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNI-YMDTGPQLPTNVTHMLKLGSNL 240

Query: 241 LQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPIS 300
           LQ +G+FNGHY IAVA++G+APSPDSS+LQDH QPVVSTVDSDSDIIEGPSRISLNCPIS
Sbjct: 241 LQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSDSDIIEGPSRISLNCPIS 300

Query: 301 YTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLRE 360
           YTRIKVPVKG SCKHLQCFDF NFIDINSRRPSWRCPHCNQYIC+LDI +DQNMLKV+RE
Sbjct: 301 YTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLKVIRE 360

Query: 361 VGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDN 420
           V ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  AQQESTAPPDVLDLTEVDD+
Sbjct: 361 VAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDD 420

Query: 421 MNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--DETLT 480
           MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFWSG+  D  LT
Sbjct: 421 MNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLLT 480

Query: 481 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQ-NLQV 540
           S  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  PA +DQ N+QV
Sbjct: 481 SSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQV 540

Query: 541 QALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGH--- 600
           Q  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTSTISSAPQVG    
Sbjct: 541 QVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVGQSIP 600

Query: 601 ----GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQAS 660
               GLNTI+RDSERR  FP HHGD H ATNLAPF RPP VQ R+PQDRSFTPG +V+AS
Sbjct: 601 ISRDGLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRAS 660

Query: 661 TPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAY 720
           T  RPS G+LT+FQNPHLQQALN+R+  LRNQ P++VRPSL   R  +QV GGGYGG+AY
Sbjct: 661 TAQRPSAGILTDFQNPHLQQALNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYGGSAY 720

Query: 721 ATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVG 780
             VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R   AG++RNVG
Sbjct: 721 PAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR-RPAGDLRNVG 780

Query: 781 GMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNP 840
           GM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ VQSARP SN 
Sbjct: 781 GMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQPVQSARPPSNL 840

Query: 841 TPTPPSTASTQAQMFNGRDTQVPRT 854
           T T  S  ST AQ  NG DT +PRT
Sbjct: 841 TTTQSSAPSTHAQRSNGFDTVIPRT 860

BLAST of Moc06g33130 vs. NCBI nr
Match: XP_022928990.1 (E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 638/865 (73.76%), Postives = 713/865 (82.43%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMVLMI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +NSLTTITTVMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNL 240
            LFV QKDN ETSAC+I+PPQVNFLVNGRGVNGRTN  Y DTGPQLPTN+  MLKLGSNL
Sbjct: 181 RLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNI-YMDTGPQLPTNVTHMLKLGSNL 240

Query: 241 LQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPIS 300
           LQ +G+FNGHY IAVA++G+APSPDSS+LQDH QPVVSTVDSDSDIIEGPSRISLNCPIS
Sbjct: 241 LQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSDSDIIEGPSRISLNCPIS 300

Query: 301 YTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLRE 360
           YTRIKVPVKG SCKHLQCFDF NFIDINSRRPSWRCPHCNQYIC+LDI +DQNMLKV+RE
Sbjct: 301 YTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLKVIRE 360

Query: 361 VGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDN 420
           V ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  AQQESTAPPDVLDLTEVDD+
Sbjct: 361 VAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDD 420

Query: 421 MNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--DETLT 480
           MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFWSG+  D  L 
Sbjct: 421 MNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLLI 480

Query: 481 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQ-NLQV 540
           S  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  PA +DQ N+QV
Sbjct: 481 SSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQV 540

Query: 541 QALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGH--- 600
           Q  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTSTISSAPQVG    
Sbjct: 541 QVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVGQSIP 600

Query: 601 ----GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQAS 660
               GLNTI+RDSERR  FP HHGD H ATNLAPF RPP VQ R+PQDRSFTPG +V+AS
Sbjct: 601 ISRDGLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRAS 660

Query: 661 TPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAY 720
           T  RPS G+LT+FQNPHLQQ+LN+R+  LRNQ P++VRPSL   R  +QV GGGYGG+AY
Sbjct: 661 TAQRPSAGILTDFQNPHLQQSLNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYGGSAY 720

Query: 721 ATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVG 780
             VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R   AG++RNVG
Sbjct: 721 PAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR-RPAGDLRNVG 780

Query: 781 GMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNP 840
           GM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ VQS RP SN 
Sbjct: 781 GMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQPVQSTRPPSNL 840

Query: 841 TPTPPSTASTQAQMFNGRDTQVPRT 854
           T T  +  ST AQ  NG DT VPRT
Sbjct: 841 TTTQSNAPSTHAQRSNGFDTVVPRT 860

BLAST of Moc06g33130 vs. NCBI nr
Match: XP_022969988.1 (E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 635/865 (73.41%), Postives = 712/865 (82.31%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMVLMI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +NSLTTIT VMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNSLTTITKVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNL 240
            LFV QKDN ETSAC+I+PPQVNFLVNGRGVNGRTN  Y DTGPQLPTN+  MLKLGSNL
Sbjct: 181 RLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNI-YMDTGPQLPTNVTHMLKLGSNL 240

Query: 241 LQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPIS 300
           LQ +G+FNGHY I+VA++G+APSPDSS+LQDH QP VSTVDSDSDIIEGPSRISLNCPIS
Sbjct: 241 LQVIGSFNGHYVISVAVMGSAPSPDSSVLQDHEQPAVSTVDSDSDIIEGPSRISLNCPIS 300

Query: 301 YTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLRE 360
           YTRIKVPVKG SCKHLQCFDF NFIDINSRRPSWRCPHCNQYIC+LDI +DQNMLKV+RE
Sbjct: 301 YTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLKVIRE 360

Query: 361 VGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDN 420
           V ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  AQQESTAPPDVLDLTEVDD+
Sbjct: 361 VAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDD 420

Query: 421 MNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--DETLT 480
           MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFWS +  D  LT
Sbjct: 421 MNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSRMVTDRLLT 480

Query: 481 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQ-NLQV 540
           S  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  P+ +DQ N+QV
Sbjct: 481 SSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPSFYDQNNVQV 540

Query: 541 QALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGH--- 600
           Q  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTST+SSAPQVG    
Sbjct: 541 QVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTVSSAPQVGQSIP 600

Query: 601 ----GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQAS 660
               GLNTI+RDSE R  FP HHGD H ATNLAPF RPP VQ R+PQDRSFTPG +V+AS
Sbjct: 601 ISRDGLNTISRDSEMRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRAS 660

Query: 661 TPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAY 720
           T  RPS+G+LT+FQNPHLQQALN+R+  L+NQ P++VRPSL   R  +QV GGGYGG+AY
Sbjct: 661 TAQRPSVGILTDFQNPHLQQALNLRISHLQNQNPSSVRPSLPFSRPTSQV-GGGYGGSAY 720

Query: 721 ATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVG 780
             VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R   AGE+RNVG
Sbjct: 721 TAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR-RPAGELRNVG 780

Query: 781 GMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNP 840
           GM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ VQSARP SN 
Sbjct: 781 GMTQSVTMASNLLDPSVEQNRQPIGRMRGSLSGRAYSDAFG-VIIQPTQPVQSARPPSNL 840

Query: 841 TPTPPSTASTQAQMFNGRDTQVPRT 854
           T T  S  ST AQ  NG DT VPRT
Sbjct: 841 TTTQSSAPSTHAQRSNGFDTVVPRT 860

BLAST of Moc06g33130 vs. NCBI nr
Match: XP_023550945.1 (E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 630/865 (72.83%), Postives = 706/865 (81.62%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMV+MI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVVMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +N+L TITTVMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNALATITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNL 240
            LFV QKDN ETSAC+I+PPQVNFLVNGRGVNGRTN  Y DTGPQLPTN+  MLKLGSNL
Sbjct: 181 RLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNI-YMDTGPQLPTNVTHMLKLGSNL 240

Query: 241 LQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPIS 300
           LQ +G+FNGHY IAVA++G+APSPDSS+LQDH QP VSTVDSDSDIIEGPSRISLNCPIS
Sbjct: 241 LQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPAVSTVDSDSDIIEGPSRISLNCPIS 300

Query: 301 YTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLRE 360
           YTRIKVPVKG SCKHLQ   F NFIDINSRRPSWRCPHCNQYIC+LDI +D+NMLKV+RE
Sbjct: 301 YTRIKVPVKGRSCKHLQLLXFYNFIDINSRRPSWRCPHCNQYICFLDICIDRNMLKVIRE 360

Query: 361 VGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDN 420
           V ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  A+QESTAPPDVLDLTEVDD+
Sbjct: 361 VAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAEQESTAPPDVLDLTEVDDD 420

Query: 421 MNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--DETLT 480
           MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFWSG+  D  LT
Sbjct: 421 MNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLLT 480

Query: 481 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQ-NLQV 540
           S  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  PA +DQ N+QV
Sbjct: 481 SSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQV 540

Query: 541 QALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGH--- 600
           Q  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTSTISSAPQVG    
Sbjct: 541 QVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVGQSIP 600

Query: 601 ----GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQAS 660
               GLN I+RDSERR  FP HHGD H ATNLAPF RPP VQ R+PQD SFTPG +V+AS
Sbjct: 601 ISRDGLNMISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDCSFTPGQSVRAS 660

Query: 661 TPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAY 720
           T  RPS G+LT+FQNPHLQQALN+R+  LRNQ P++VRPSL   R  +QV GGGYGG+AY
Sbjct: 661 TAQRPSAGILTDFQNPHLQQALNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYGGSAY 720

Query: 721 ATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVG 780
             VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R    GE+RNVG
Sbjct: 721 TAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR-RPTGELRNVG 780

Query: 781 GMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNP 840
           GM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ VQSARP SN 
Sbjct: 781 GMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQPVQSARPPSNL 840

Query: 841 TPTPPSTASTQAQMFNGRDTQVPRT 854
           T T  S  ST  Q  NG DT VPRT
Sbjct: 841 TTTQSSAPSTHTQRSNGFDTVVPRT 860

BLAST of Moc06g33130 vs. NCBI nr
Match: KAG7016993.1 (E4 SUMO-protein ligase PIAL2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 628/875 (71.77%), Postives = 705/875 (80.57%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMVLMI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +NSLTTITTVMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFL-VNGRGVNGRTNTGYT------DTGPQLPTNIACM 240
            LFV QKDN ETSAC+I+PPQV +L     G   +    +       DTGPQLPTN+  M
Sbjct: 181 RLFVAQKDNTETSACIISPPQVKYLPCQWEGSQWKDKYIHASFLFNKDTGPQLPTNVTHM 240

Query: 241 LKLGSNLLQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRI 300
           LKLGSNLLQ +G+FNGHY IAVA++G+APSPDSS+LQDH QPVVSTVDSDSDIIEGPSRI
Sbjct: 241 LKLGSNLLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSDSDIIEGPSRI 300

Query: 301 SLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQN 360
           SLNCPISYTRIKVPVKG SCKHLQCFDF NFIDINSRRPSWRCPHCNQYIC+LDI +DQN
Sbjct: 301 SLNCPISYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQN 360

Query: 361 MLK---VLREVGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPD 420
           MLK   V+REV ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  AQQESTAPPD
Sbjct: 361 MLKASLVIREVAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPD 420

Query: 421 VLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFW 480
           VLDLTEVDD+MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFW
Sbjct: 421 VLDLTEVDDDMNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFW 480

Query: 481 SGI--DETLTSITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSP 540
           SG+  D  LTS  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  P
Sbjct: 481 SGMVTDRLLTSSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFP 540

Query: 541 ALHDQ-NLQVQALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTIS 600
           A +DQ N+QVQ  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTSTIS
Sbjct: 541 AFYDQNNVQVQVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTIS 600

Query: 601 SAPQVGH-------GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRS 660
           SAPQVG        GLNTI+RDSERR  FP HHGD H ATNLAPF RPP VQ R+PQDRS
Sbjct: 601 SAPQVGQSIPISRDGLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRS 660

Query: 661 FTPGLTVQASTPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQV 720
           FTPG +V+AST  RPS G+LT+FQNPHLQQALN+R+  LRNQ P++VRPSL   R  +QV
Sbjct: 661 FTPGQSVRASTAQRPSAGILTDFQNPHLQQALNLRISHLRNQNPSSVRPSLPFSRPTSQV 720

Query: 721 GGGGYGGAAYATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRT 780
            GGGYGG+AY  VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R 
Sbjct: 721 -GGGYGGSAYPAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR- 780

Query: 781 AAAGEVRNVGGMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQS 840
             AG++RNVGGM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ 
Sbjct: 781 RPAGDLRNVGGMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQP 840

Query: 841 VQSARPSSNPTPTPPSTASTQAQMFNGRDTQVPRT 854
           VQSARP SN T T  S  ST AQ  NG DT +PRT
Sbjct: 841 VQSARPPSNLTTTQSSAPSTHAQRSNGFDTVIPRT 871

BLAST of Moc06g33130 vs. ExPASy Swiss-Prot
Match: F4JYG0 (E4 SUMO-protein ligase PIAL2 OS=Arabidopsis thaliana OX=3702 GN=PIAL2 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.7e-112
Identity = 287/848 (33.84%), Postives = 416/848 (49.06%), Query Frame = 0

Query: 9   LNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQAHSLPGLLK 68
           +N  ++A++ + L  +++   + DP +F   CIS A+ ID +IANN +P +    P LLK
Sbjct: 24  VNSFRLASVTQRLRYHIQDGAKVDPKEFQICCISFAKGIDFAIANNDIPKKVEEFPWLLK 83

Query: 69  QICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDF--FGDVNTGQ 128
           Q+C+      TK A+MVLMISVK AC + WFS+ E++EL +LA+EI + F   G  + G 
Sbjct: 84  QLCRHGTDVYTKTALMVLMISVKHACHLGWFSDSESQELIALADEIRTCFGSSGSTSPGI 143

Query: 129 TNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKILLFVVQ 188
            +  +T + +MERF+P +KLG ++ S EVK GY + A DF ISK   +S QEKI LFV Q
Sbjct: 144 KSPGSTFSQIMERFYPFVKLGHVLVSFEVKAGYTMLAHDFYISKNMPHSLQEKIRLFVAQ 203

Query: 189 KDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNLLQAVGN 248
            DNI+TSAC+  PP+V+FL+NG+GV  R N    DTGPQLPTN+   LK G+NLLQ +GN
Sbjct: 204 TDNIDTSACISNPPEVSFLLNGKGVEKRVNIA-MDTGPQLPTNVTAQLKYGTNLLQVMGN 263

Query: 249 FNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKV 308
           F G+Y I +A  G    P+  +L+D++Q  V     DSDIIEGPSR+SL+CPIS  RIK+
Sbjct: 264 FKGNYIIIIAFTGLVVPPEKPVLKDYLQSGVIEASPDSDIIEGPSRVSLSCPISRKRIKL 323

Query: 309 PVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVT 368
           PVKG  CKHLQCFDF N++ IN R P+WRCPHCNQ +CY DIR+DQNM K+L++V  N  
Sbjct: 324 PVKGQLCKHLQCFDFSNYVHINMRNPTWRCPHCNQPVCYPDIRLDQNMAKILKDVEHNAA 383

Query: 369 EVIISADGSWKAILNDYGDGRP-------LEDSLKNQNGGAQQESTAPPDVLDLTEVDD- 428
           +VII A G+WK   N      P       LED +   N G        P V DLT  DD 
Sbjct: 384 DVIIDAGGTWKVTKNTGETPEPVREIIHDLEDPMSLLNSG--------PVVFDLTGDDDA 443

Query: 429 NMNIC-NLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGIDETLT 488
            + +  + + EDRKPC+S+     +   + ++  N++  N +++++ +      +D  + 
Sbjct: 444 ELEVFGDNKVEDRKPCMSD-----AQGQSNNNNTNKHPSNDDYSSIFDISDVIALDPEIL 503

Query: 489 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQNLQVQ 548
           S       LGN+ P                                             Q
Sbjct: 504 S------ALGNTAPQPH------------------------------------------Q 563

Query: 549 ALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGHGLNT 608
           A N+    QY  ++ IP  +   PV V   P     S +     TST+ + P        
Sbjct: 564 ASNTGTGQQYSNLSQIPMSIDPMPVPV---PFSQTPSPRDRPATTSTVFTIPNP------ 623

Query: 609 ITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQASTPLRPSLG 668
                                                      +P  +   ++P+ P+  
Sbjct: 624 -------------------------------------------SPQYSQVHASPVTPTGT 683

Query: 669 LLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAYATVTSSQC 728
            L    +P              NQ   +  P + +P T  +V            VTS   
Sbjct: 684 YLGRTTSPRW------------NQTYQSQAPPMTTPYTSRKVS---------VPVTSQSP 725

Query: 729 ARM--MVTSQRA-EMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVGGMSQSV 788
           A +   V SQ    +L Q     +   TS  A + +  P G    +   + ++  +  +V
Sbjct: 744 ANVSSFVQSQHVPRVLSQPNNYGVRGLTSSHASTSRQHPSGPTVQSVSRLSDLVDVDLTV 725

Query: 789 PTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQ-SARPSSNPTPTPP 842
           P ++         NW+P  RMRGSL   ++S AL  ++I+P+Q  Q S R +S+     P
Sbjct: 804 PDTS---------NWRP--RMRGSLVPGSHSTALDHMIIRPSQQSQTSTRLNSSQPVQTP 725

BLAST of Moc06g33130 vs. ExPASy Swiss-Prot
Match: A0A0A7EPL0 (E4 SUMO-protein ligase PIAL1 OS=Arabidopsis thaliana OX=3702 GN=PIAL1 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 2.6e-102
Identity = 281/830 (33.86%), Postives = 416/830 (50.12%), Query Frame = 0

Query: 30  QFDPGQFYSICISLARSIDLSIANNKVPSQAHSLPGLLKQICQKK-HTHQTKAAIMVLMI 89
           +F+  +F + CISLA  ID +I  N+VP     L  +L  +C++K   +QT+A +M LMI
Sbjct: 14  EFNTKEFQASCISLANEIDAAIGRNEVPGNIQELALILNNVCRRKCDDYQTRAVVMALMI 73

Query: 90  SVKSACKMRWFSEKEAEELYSLANEIGSDFFGDVN-TGQTNS-LTTITTVMERFFPRMKL 149
           SVKSAC++ WF E+E +EL ++ + + + F    N T   NS +T I+ V+ERF+P +KL
Sbjct: 74  SVKSACQLGWFPERETQELLAIIDLMWNGFSCPENVTSCVNSPVTLISQVIERFYPCVKL 133

Query: 150 GQIIASVEVKPGYGVFATDFNISKTTQYSQQEKILLFVVQKDNIETSACLITPPQVNFLV 209
           G I+ S E KP   +   DF+ISK   +S ++K+ LFVV+ ++I  S C++ P  V+FL+
Sbjct: 134 GHILVSFEAKPESKMMMKDFHISKKMPHSPKQKVGLFVVRTEDISRSNCIVHPQGVSFLL 193

Query: 210 NGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNLLQAVGNFNGHYAIAVAIIGTAPSPDS 269
           NG+G++ R N    ++GPQLPTN+  +L LG+NLLQA+G F G Y IA+A +   P P+ 
Sbjct: 194 NGKGIDKRVNIS-MESGPQLPTNVTALLNLGANLLQAIGCFGGSYLIAIAFMDVIPLPNK 253

Query: 270 SMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFID 329
            +L+D+V P V   +SD DIIEGPSRISL+CPIS TRIK+PVKG  CKHLQCFDF N+++
Sbjct: 254 PLLKDYVHPEVVGSNSDCDIIEGPSRISLSCPISRTRIKLPVKGHVCKHLQCFDFWNYVN 313

Query: 330 INSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYGDG 389
           +N+RRPSWRCPHCNQ +CY DIRVDQ + K+L EVG N  +V+ISADG+W  +  +  + 
Sbjct: 314 MNTRRPSWRCPHCNQSVCYTDIRVDQKLRKILEEVGRNAADVVISADGTW-MVETENDED 373

Query: 390 RPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLN 449
             L     + +G         P V +    D+N    + + E+  PCLS    P + +  
Sbjct: 374 VELVPETTHDHGDPNSFINLGPTVKNPAR-DENEMETSTQVEEHNPCLSEIQGPSNDTHR 433

Query: 450 ISSG---MNRNSLNQNFAAVLEDDFWSGIDETLTS----ITRSDAPLGNSTPATSSADLM 509
            +S    +N++  + N    L     +   +   +    I   D+P   + P T S    
Sbjct: 434 PASDYTMLNQSHTSTNTLPQLPRTLNAFDGQQFVNLPQVINTRDSPASQALPMTFSPTPS 493

Query: 510 QSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQNL-----QVQALNSNENNQYGRMTLIP 569
              +L    A   N G  +P   +      H  +L     +   L +  N+ YGR+    
Sbjct: 494 PQDILATNAA---NFGTSMPAAQSSQFQGSHVTSLGNCEGRTSDLMARWNHIYGRVQTQF 553

Query: 570 RPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQ---VGHGLNTITRDSERRL----Q 629
            P    P++      Q+Q           +  + PQ   V +G N   R     +     
Sbjct: 554 PP---APLSHHHYSMQNQSPSPAQQRPVPSYIAHPQTFHVNYGENADQRWMPSSIAHPQT 613

Query: 630 FPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQASTPLRPSLGLLTEFQNPHL 689
            P+++G     TN    QRP    I  PQ    T  +  + +T  R      T +   HL
Sbjct: 614 LPVNYGG---NTN----QRPIPSSIAHPQ----TLPVNYRGNTDHRS-----TPYSITHL 673

Query: 690 QQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAYATVTSSQCARMMVTSQRA 749
           Q  LN      +  +P+++      P T        YGG A+    SS      +T  R 
Sbjct: 674 QTLLNYGGNADQRPMPSSITNLQTLPAT--------YGGYAHQRPMSSS-----ITHPRT 733

Query: 750 EMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVGG-MSQSVPTSAGLVEPSLE 809
             +            S   H  QT P  +      ++ N GG M Q        + P+  
Sbjct: 734 SPVNYGGTPDQRPMPSSITHP-QTLPVSY-GGTTDQILNPGGAMGQFSSREFMNLTPANT 793

Query: 810 QNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNPTPTPPSTA 837
           +NW+P  RMRGS+   T  D    ++I PT+ V    P +   P P ST+
Sbjct: 794 ENWRPQSRMRGSVAPGTGYD---HMIIHPTRPV---HPQAQTPPAPLSTS 797

BLAST of Moc06g33130 vs. ExPASy Swiss-Prot
Match: O94451 (E3 SUMO-protein ligase pli1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pli1 PE=1 SV=3)

HSP 1 Score: 102.8 bits (255), Expect = 1.9e-20
Identity = 106/447 (23.71%), Postives = 175/447 (39.15%), Query Frame = 0

Query: 168 ISKTTQYSQQEKILLFVVQKDNIETSACLI--TPPQVNFLVNGRGVNGRTNTGYTDTGPQ 227
           +SK     +Q ++ LF    + I    CL+    PQ+   +N +  +          G  
Sbjct: 161 LSKLLNDPKQYRVYLFSTPSETIGFGNCLMEFPTPQMELRINNQVAHANYRRLKGKPGTT 220

Query: 228 LPTNIACMLKL-----GSNLLQAVGNFNGHYAIAVAIIGTAPSPD-----SSMLQDHVQP 287
            P +I  ++       G+N++    N    Y++ V  +      +      S   +  + 
Sbjct: 221 NPADITDLVSKYAGPPGNNVVIYYMNSTKSYSVVVCFVKVYTIENLVDQIKSRKAESKEK 280

Query: 288 VVSTV---DSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRP 347
           ++  +   + D+DII   + ISL CP+S++RI +PV+   CKH+QCFD   F+++N + P
Sbjct: 281 IIERIKNDNQDADIIATSTDISLKCPLSFSRISLPVRSVFCKHIQCFDASAFLEMNKQTP 340

Query: 348 SWRCPHCNQYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILND---------- 407
           SW CP C  +I + D+ +D  M  +L     N   + +  +G+WK    D          
Sbjct: 341 SWMCPVCASHIQFSDLIIDGFMQHILESTPSNSETITVDPEGNWKLNTFDEPVESSEDEF 400

Query: 408 --------YGDGRPLEDSLKNQNG----GAQQESTAPPD-------VLDLTEVDDNMNIC 467
                     DG  +       N      A   ++ PP        V+DLT  DD+ N+ 
Sbjct: 401 VPKEKVIELSDGEGISTMANKSNDQPTRRASTHNSGPPAKRKRESLVIDLTISDDDENVA 460

Query: 468 NLETEDRKPCLSNKNQPVS---SSLNISSGMNRNSLNQNFAAVLEDDFWSGIDETLTSIT 527
              TE   P  + K   +S    S NI + ++  S N         D+           T
Sbjct: 461 TSTTE--SPSNATKENSLSRNVQSPNIDTAISNRSTNVRHGHPGFKDY-----------T 520

Query: 528 RSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQNLQVQALN 564
             ++P       + SA   QS+V            +G  G     S AL   + Q    N
Sbjct: 521 VENSPASRERSTSESA---QSSV-----------HMGYAGEGGLLSGALRAPS-QQNNNN 579

BLAST of Moc06g33130 vs. ExPASy Swiss-Prot
Match: Q04195 (E3 SUMO-protein ligase SIZ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SIZ1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.4e-18
Identity = 57/179 (31.84%), Postives = 92/179 (51.40%), Query Frame = 0

Query: 281 DSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCN 340
           D +  +    + +SL CPISYTR+K P K  +CKHLQCFD   F+    + P+W+CP C 
Sbjct: 345 DEEMGLTTTSTIMSLQCPISYTRMKYPSKSINCKHLQCFDALWFLHSQLQIPTWQCPVCQ 404

Query: 341 QYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYGDGRPLEDSLKNQNGGA 400
             I   ++ + + +  +L+   +NV +V +++DG W AIL D  D     DS  N    +
Sbjct: 405 IDIALENLAISEFVDDILQNCQKNVEQVELTSDGKWTAILEDDDD----SDSDSNDGSRS 464

Query: 401 QQESTAPPDVLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQN 460
            ++ T+  D    +       I NL+++D +P   N N P  ++ +  S  + N  N N
Sbjct: 465 PEKGTSVSDHHCSSSHPSEPIIINLDSDDDEP---NGNNPHVTNNHDDSNRHSNDNNNN 516

BLAST of Moc06g33130 vs. ExPASy Swiss-Prot
Match: Q12216 (E3 SUMO-protein ligase SIZ2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=NFI1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.4e-18
Identity = 48/124 (38.71%), Postives = 71/124 (57.26%), Query Frame = 0

Query: 283 DSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQY 342
           D DII   + +SL CPIS TR+K P K   CKH+QCFD   F+   S+ P+W+CP C   
Sbjct: 324 DDDIITTSTVLSLQCPISCTRMKYPAKTDQCKHIQCFDALWFLHSQSQVPTWQCPICQHP 383

Query: 343 ICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYG---DGRPLEDSLKNQNGG 402
           I +  +++ + +  +++   E+V +V IS DGSWK I N      D      S+KN+N G
Sbjct: 384 IKFDQLKISEFVDNIIQNCNEDVEQVEISVDGSWKPIHNSSAVITDTVNQNHSVKNENQG 443

Query: 403 AQQE 404
             ++
Sbjct: 444 TVKQ 447

BLAST of Moc06g33130 vs. ExPASy TrEMBL
Match: A0A6J1ESZ6 (E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435722 PE=4 SV=1)

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 638/865 (73.76%), Postives = 713/865 (82.43%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMVLMI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +NSLTTITTVMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNL 240
            LFV QKDN ETSAC+I+PPQVNFLVNGRGVNGRTN  Y DTGPQLPTN+  MLKLGSNL
Sbjct: 181 RLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNI-YMDTGPQLPTNVTHMLKLGSNL 240

Query: 241 LQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPIS 300
           LQ +G+FNGHY IAVA++G+APSPDSS+LQDH QPVVSTVDSDSDIIEGPSRISLNCPIS
Sbjct: 241 LQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSDSDIIEGPSRISLNCPIS 300

Query: 301 YTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLRE 360
           YTRIKVPVKG SCKHLQCFDF NFIDINSRRPSWRCPHCNQYIC+LDI +DQNMLKV+RE
Sbjct: 301 YTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLKVIRE 360

Query: 361 VGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDN 420
           V ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  AQQESTAPPDVLDLTEVDD+
Sbjct: 361 VAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDD 420

Query: 421 MNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--DETLT 480
           MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFWSG+  D  L 
Sbjct: 421 MNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLLI 480

Query: 481 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQ-NLQV 540
           S  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  PA +DQ N+QV
Sbjct: 481 SSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQV 540

Query: 541 QALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGH--- 600
           Q  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTSTISSAPQVG    
Sbjct: 541 QVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVGQSIP 600

Query: 601 ----GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQAS 660
               GLNTI+RDSERR  FP HHGD H ATNLAPF RPP VQ R+PQDRSFTPG +V+AS
Sbjct: 601 ISRDGLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRAS 660

Query: 661 TPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAY 720
           T  RPS G+LT+FQNPHLQQ+LN+R+  LRNQ P++VRPSL   R  +QV GGGYGG+AY
Sbjct: 661 TAQRPSAGILTDFQNPHLQQSLNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYGGSAY 720

Query: 721 ATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVG 780
             VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R   AG++RNVG
Sbjct: 721 PAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR-RPAGDLRNVG 780

Query: 781 GMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNP 840
           GM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ VQS RP SN 
Sbjct: 781 GMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQPVQSTRPPSNL 840

Query: 841 TPTPPSTASTQAQMFNGRDTQVPRT 854
           T T  +  ST AQ  NG DT VPRT
Sbjct: 841 TTTQSNAPSTHAQRSNGFDTVVPRT 860

BLAST of Moc06g33130 vs. ExPASy TrEMBL
Match: A0A6J1I2J0 (E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469015 PE=4 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 635/865 (73.41%), Postives = 712/865 (82.31%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMVLMI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +NSLTTIT VMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNSLTTITKVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNL 240
            LFV QKDN ETSAC+I+PPQVNFLVNGRGVNGRTN  Y DTGPQLPTN+  MLKLGSNL
Sbjct: 181 RLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNI-YMDTGPQLPTNVTHMLKLGSNL 240

Query: 241 LQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPIS 300
           LQ +G+FNGHY I+VA++G+APSPDSS+LQDH QP VSTVDSDSDIIEGPSRISLNCPIS
Sbjct: 241 LQVIGSFNGHYVISVAVMGSAPSPDSSVLQDHEQPAVSTVDSDSDIIEGPSRISLNCPIS 300

Query: 301 YTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLRE 360
           YTRIKVPVKG SCKHLQCFDF NFIDINSRRPSWRCPHCNQYIC+LDI +DQNMLKV+RE
Sbjct: 301 YTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLKVIRE 360

Query: 361 VGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDN 420
           V ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  AQQESTAPPDVLDLTEVDD+
Sbjct: 361 VAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDD 420

Query: 421 MNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--DETLT 480
           MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFWS +  D  LT
Sbjct: 421 MNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSRMVTDRLLT 480

Query: 481 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQ-NLQV 540
           S  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  P+ +DQ N+QV
Sbjct: 481 SSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPSFYDQNNVQV 540

Query: 541 QALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGH--- 600
           Q  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTST+SSAPQVG    
Sbjct: 541 QVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTVSSAPQVGQSIP 600

Query: 601 ----GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQAS 660
               GLNTI+RDSE R  FP HHGD H ATNLAPF RPP VQ R+PQDRSFTPG +V+AS
Sbjct: 601 ISRDGLNTISRDSEMRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRAS 660

Query: 661 TPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAY 720
           T  RPS+G+LT+FQNPHLQQALN+R+  L+NQ P++VRPSL   R  +QV GGGYGG+AY
Sbjct: 661 TAQRPSVGILTDFQNPHLQQALNLRISHLQNQNPSSVRPSLPFSRPTSQV-GGGYGGSAY 720

Query: 721 ATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVG 780
             VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R   AGE+RNVG
Sbjct: 721 TAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR-RPAGELRNVG 780

Query: 781 GMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNP 840
           GM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ VQSARP SN 
Sbjct: 781 GMTQSVTMASNLLDPSVEQNRQPIGRMRGSLSGRAYSDAFG-VIIQPTQPVQSARPPSNL 840

Query: 841 TPTPPSTASTQAQMFNGRDTQVPRT 854
           T T  S  ST AQ  NG DT VPRT
Sbjct: 841 TTTQSSAPSTHAQRSNGFDTVVPRT 860

BLAST of Moc06g33130 vs. ExPASy TrEMBL
Match: A0A6J1EMF7 (E4 SUMO-protein ligase PIAL2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435722 PE=4 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 623/869 (71.69%), Postives = 701/869 (80.67%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M A +P E+ L++I++ I+ L L V  + Q DP Q  +IC SLARSID +IAN+ VPS+A
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
             LP LLKQICQKKH+H  KAAIMVLMI+ K+ACK++WFSEKEAEELYSLANEIGSDFF 
Sbjct: 61  QGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDFFV 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG +NSLTTITTVMERFFPR+KLGQI+ S EVKPGYGVFA DFNISKT QY+ QEKI
Sbjct: 121 DTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQEKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGR----TNTGYTDTGPQLPTNIACMLKL 240
            LFV QKDN ETSAC+I+PPQ+     G     +    +     DTGPQLPTN+  MLKL
Sbjct: 181 RLFVAQKDNTETSACIISPPQLPCQWEGSQWKDKYIHASFLFNKDTGPQLPTNVTHMLKL 240

Query: 241 GSNLLQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLN 300
           GSNLLQ +G+FNGHY IAVA++G+APSPDSS+LQDH QPVVSTVDSDSDIIEGPSRISLN
Sbjct: 241 GSNLLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSDSDIIEGPSRISLN 300

Query: 301 CPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLK 360
           CPISYTRIKVPVKG SCKHLQCFDF NFIDINSRRPSWRCPHCNQYIC+LDI +DQNMLK
Sbjct: 301 CPISYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLK 360

Query: 361 VLREVGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTE 420
           V+REV ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN  AQQESTAPPDVLDLTE
Sbjct: 361 VIREVAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTE 420

Query: 421 VDDNMNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--D 480
           VDD+MNICNLETEDRKPCL NKNQPVSSSLNI SGMNRNSLNQNF+A L+DDFWSG+  D
Sbjct: 421 VDDDMNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTD 480

Query: 481 ETLTSITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQ- 540
             L S  RSDAP+G+ST A S A L QSA LT+AV+PVLNH VGVPG   F  PA +DQ 
Sbjct: 481 RLLISSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQN 540

Query: 541 NLQVQALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVG 600
           N+QVQ  NSNE+NQYGRMT I RPVSRT +  Q LPAQSQ SGQ YS RTSTISSAPQVG
Sbjct: 541 NVQVQVSNSNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVG 600

Query: 601 H-------GLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLT 660
                   GLNTI+RDSERR  FP HHGD H ATNLAPF RPP VQ R+PQDRSFTPG +
Sbjct: 601 QSIPISRDGLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQS 660

Query: 661 VQASTPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYG 720
           V+AST  RPS G+LT+FQNPHLQQ+LN+R+  LRNQ P++VRPSL   R  +QV GGGYG
Sbjct: 661 VRASTAQRPSAGILTDFQNPHLQQSLNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYG 720

Query: 721 GAAYATVT-SSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEV 780
           G+AY  VT  +Q ARMMV SQRAEM++QS+AMSL NQTSRS H LQTTPDG R   AG++
Sbjct: 721 GSAYPAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLR-RPAGDL 780

Query: 781 RNVGGMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARP 840
           RNVGGM+QSV  ++ L++PS+EQN QP GRMRGSL+GR YSDA G ++IQPTQ VQS RP
Sbjct: 781 RNVGGMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQPVQSTRP 840

Query: 841 SSNPTPTPPSTASTQAQMFNGRDTQVPRT 854
            SN T T  +  ST AQ  NG DT VPRT
Sbjct: 841 PSNLTTTQSNAPSTHAQRSNGFDTVVPRT 865

BLAST of Moc06g33130 vs. ExPASy TrEMBL
Match: A0A6J1K8Y8 (E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491198 PE=4 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 621/867 (71.63%), Postives = 687/867 (79.24%), Query Frame = 0

Query: 1   MDAPSPCELNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQA 60
           M  P P E+  N+I+  I+G+  +V    Q DP  F ++C SLAR ID +IANN VPS  
Sbjct: 1   MGTPLPYEMYSNRISLYIDGITSHVNRYDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HSLPGLLKQICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDFFG 120
           H LP LLKQ+ QKKH+H+ KAA+MVLMIS K+ACK+RWFSEKEAE+LYSLANEIGSDFFG
Sbjct: 61  HGLPNLLKQMYQKKHSHRLKAAVMVLMISTKNACKVRWFSEKEAEDLYSLANEIGSDFFG 120

Query: 121 DVNTGQTNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKI 180
           D NTG  NSLTTIT VMERFFP +KLGQI+A++EVKPGYGVFATDFNISKT Q+SQQ+KI
Sbjct: 121 DTNTGPNNSLTTITAVMERFFPHLKLGQIVAAMEVKPGYGVFATDFNISKTMQFSQQDKI 180

Query: 181 LLFVVQKDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNL 240
           LLFV QKDN ETSAC+I+PPQVNFLVNG+GVNGRTN  + DTGPQLPTN+  MLKLG+NL
Sbjct: 181 LLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNI-FMDTGPQLPTNVTHMLKLGANL 240

Query: 241 LQAVGNFNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPIS 300
           LQA+GNFNGHY IAVAI+GTAP PDSS+LQD+VQPVVSTVDSDSDIIEGPSRISLNCPIS
Sbjct: 241 LQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQPVVSTVDSDSDIIEGPSRISLNCPIS 300

Query: 301 YTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLRE 360
           YTRIKVPVK  SCKHLQCFDF NFI INSRRPSWRCPHCNQYIC+LDIRVDQNM+KV+RE
Sbjct: 301 YTRIKVPVKSRSCKHLQCFDFYNFIGINSRRPSWRCPHCNQYICFLDIRVDQNMMKVIRE 360

Query: 361 VGENVTEVIISADGSWKAIL-NDYGDGRPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDN 420
           V ENVTEVIISADGSWKAIL ND GDGRPL+DSL  QN    QEST  PDVLDL EVDD+
Sbjct: 361 VAENVTEVIISADGSWKAILENDNGDGRPLDDSLNLQN-ERDQESTV-PDVLDLIEVDDD 420

Query: 421 MNICNLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGI--DETLT 480
           +NIC+LE ED KPCL NK                     NFAAVL+DDFWSGI  D  LT
Sbjct: 421 INICDLEIEDEKPCLGNK---------------------NFAAVLDDDFWSGIDTDRILT 480

Query: 481 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVL-NHGVGVPGHAAFSSPALHDQ-NLQ 540
           S  R+DAP+GN+ PA + A LMQSAVLT  V PVL NHG GVPGH  F SPAL+DQ NLQ
Sbjct: 481 SSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFLSPALYDQNNLQ 540

Query: 541 VQALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVG--- 600
            QALNSNEN +YGR T I RP+SR P T QALP  SQ SGQ YS RT+TISSA QVG   
Sbjct: 541 TQALNSNENTEYGRTTSIARPLSRMPTTAQALPYPSQASGQQYSSRTTTISSASQVGPSI 600

Query: 601 ----HGLNTITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQA 660
                GLNTI+RDSER  QFP H GD H ATNLAPF  PPT Q RDP   SFTPG +VQA
Sbjct: 601 PTNRDGLNTISRDSERSQQFPRHPGDSHHATNLAPFHHPPTSQNRDP--HSFTPGQSVQA 660

Query: 661 STPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIP-NNVRPSLGSPRTMNQVGGGGYGGA 720
           ST LRPS  LLT+FQNPHLQQALN+RM QLRNQ P NNVRPSL   R M+QV GGGY G 
Sbjct: 661 STALRPSTRLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPFSRAMSQV-GGGYSGP 720

Query: 721 AYATVTSSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNV 780
           +YA VT +     MV SQRAE+++QS+AMSL NQT RSAHSLQTTPDG R  AAGE+RNV
Sbjct: 721 SYAAVTPNSQNARMVASQRAELMRQSSAMSLQNQTFRSAHSLQTTPDGLRMPAAGELRNV 780

Query: 781 GGMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSN 840
           GGMSQSV  +AGLV+PS EQNWQP+GRMRGSL+GR +SDA G L+I PTQSVQSARP SN
Sbjct: 781 GGMSQSVTLAAGLVDPSSEQNWQPSGRMRGSLSGRAFSDAHGHLIIHPTQSVQSARPPSN 840

Query: 841 PTPTPPSTASTQAQMFNGRDTQVPRTR 855
           PTPT PS  STQAQ  NG DT VPRTR
Sbjct: 841 PTPTQPSAPSTQAQGSNGLDTLVPRTR 840

BLAST of Moc06g33130 vs. ExPASy TrEMBL
Match: A0A6J1DSP0 (E4 SUMO-protein ligase PIAL1-like OS=Momordica charantia OX=3673 GN=LOC111023949 PE=4 SV=1)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 268 MLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDI 327
           MLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDI
Sbjct: 1   MLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDI 60

Query: 328 NSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYGDGR 387
           NSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYGDGR
Sbjct: 61  NSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYGDGR 120

Query: 388 PLEDSLKNQNGGAQQESTAPPDVLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLNI 447
           PLEDSLKNQNGGAQQESTAPPDVLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLNI
Sbjct: 121 PLEDSLKNQNGGAQQESTAPPDVLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLNI 180

Query: 448 SSGMNRNSLNQNFAAVLEDDFWSGIDETLTSITRSDAPLGNSTPATSSADLMQSAVLTEA 507
           SSGMNRNSLNQNFAAVLEDDFWSGIDETLTSITRSDAPLGNSTPATSSADLMQSAVLTEA
Sbjct: 181 SSGMNRNSLNQNFAAVLEDDFWSGIDETLTSITRSDAPLGNSTPATSSADLMQSAVLTEA 240

Query: 508 VAPVLNHGVGVPGHAAFSSPALHDQNLQVQALNSNENNQYGRMTLIPRPVSRTPVTVQAL 567
           VAPVLNHGVGVPGHAAFSSPALHDQNLQVQALNSNENNQYGRMTLIPRPVSRTPVTVQAL
Sbjct: 241 VAPVLNHGVGVPGHAAFSSPALHDQNLQVQALNSNENNQYGRMTLIPRPVSRTPVTVQAL 300

Query: 568 PAQSQGSGQLYSLRTSTISSAPQVGHGLNTITRDSERRLQFPIHHGDPHRATNLAPFQRP 627
           PAQSQGSGQLYSLRTSTISSAPQVGHGLNTITRDSERRLQFPIHHGDPHRATNLAPFQRP
Sbjct: 301 PAQSQGSGQLYSLRTSTISSAPQVGHGLNTITRDSERRLQFPIHHGDPHRATNLAPFQRP 360

Query: 628 PTVQIRDPQDRSFTPGLTVQASTPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVR 687
           PTVQIRDPQDRSFTPGLTVQASTPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVR
Sbjct: 361 PTVQIRDPQDRSFTPGLTVQASTPLRPSLGLLTEFQNPHLQQALNMRMPQLRNQIPNNVR 420

Query: 688 PSLGSPRTMNQVGGGGYGGAAYATVTSSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAH 747
           PSLGSPRTMNQVGGGGYGGAAYATVTSSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAH
Sbjct: 421 PSLGSPRTMNQVGGGGYGGAAYATVTSSQCARMMVTSQRAEMLKQSAAMSLLNQTSRSAH 480

Query: 748 SLQTTPDGHRTAAAGEVRNVGGMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDA 807
           SLQTTPDGHRTAAAGEVRNVGGMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDA
Sbjct: 481 SLQTTPDGHRTAAAGEVRNVGGMSQSVPTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDA 540

Query: 808 LGQLLIQPTQSVQSARPSSNPTPTPPSTASTQAQMFNGRDTQVPRTR 855
           LGQLLIQPTQSVQSARPSSNPTPTPPSTASTQAQMFNGRDTQVPRTR
Sbjct: 541 LGQLLIQPTQSVQSARPSSNPTPTPPSTASTQAQMFNGRDTQVPRTR 587

BLAST of Moc06g33130 vs. TAIR 10
Match: AT5G41580.1 (RING/U-box superfamily protein )

HSP 1 Score: 407.5 bits (1046), Expect = 2.6e-113
Identity = 287/848 (33.84%), Postives = 416/848 (49.06%), Query Frame = 0

Query: 9   LNLNKIAALIEGLALNVKHIGQFDPGQFYSICISLARSIDLSIANNKVPSQAHSLPGLLK 68
           +N  ++A++ + L  +++   + DP +F   CIS A+ ID +IANN +P +    P LLK
Sbjct: 24  VNSFRLASVTQRLRYHIQDGAKVDPKEFQICCISFAKGIDFAIANNDIPKKVEEFPWLLK 83

Query: 69  QICQKKHTHQTKAAIMVLMISVKSACKMRWFSEKEAEELYSLANEIGSDF--FGDVNTGQ 128
           Q+C+      TK A+MVLMISVK AC + WFS+ E++EL +LA+EI + F   G  + G 
Sbjct: 84  QLCRHGTDVYTKTALMVLMISVKHACHLGWFSDSESQELIALADEIRTCFGSSGSTSPGI 143

Query: 129 TNSLTTITTVMERFFPRMKLGQIIASVEVKPGYGVFATDFNISKTTQYSQQEKILLFVVQ 188
            +  +T + +MERF+P +KLG ++ S EVK GY + A DF ISK   +S QEKI LFV Q
Sbjct: 144 KSPGSTFSQIMERFYPFVKLGHVLVSFEVKAGYTMLAHDFYISKNMPHSLQEKIRLFVAQ 203

Query: 189 KDNIETSACLITPPQVNFLVNGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNLLQAVGN 248
            DNI+TSAC+  PP+V+FL+NG+GV  R N    DTGPQLPTN+   LK G+NLLQ +GN
Sbjct: 204 TDNIDTSACISNPPEVSFLLNGKGVEKRVNIA-MDTGPQLPTNVTAQLKYGTNLLQVMGN 263

Query: 249 FNGHYAIAVAIIGTAPSPDSSMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKV 308
           F G+Y I +A  G    P+  +L+D++Q  V     DSDIIEGPSR+SL+CPIS  RIK+
Sbjct: 264 FKGNYIIIIAFTGLVVPPEKPVLKDYLQSGVIEASPDSDIIEGPSRVSLSCPISRKRIKL 323

Query: 309 PVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVT 368
           PVKG  CKHLQCFDF N++ IN R P+WRCPHCNQ +CY DIR+DQNM K+L++V  N  
Sbjct: 324 PVKGQLCKHLQCFDFSNYVHINMRNPTWRCPHCNQPVCYPDIRLDQNMAKILKDVEHNAA 383

Query: 369 EVIISADGSWKAILNDYGDGRP-------LEDSLKNQNGGAQQESTAPPDVLDLTEVDD- 428
           +VII A G+WK   N      P       LED +   N G        P V DLT  DD 
Sbjct: 384 DVIIDAGGTWKVTKNTGETPEPVREIIHDLEDPMSLLNSG--------PVVFDLTGDDDA 443

Query: 429 NMNIC-NLETEDRKPCLSNKNQPVSSSLNISSGMNRNSLNQNFAAVLEDDFWSGIDETLT 488
            + +  + + EDRKPC+S+     +   + ++  N++  N +++++ +      +D  + 
Sbjct: 444 ELEVFGDNKVEDRKPCMSD-----AQGQSNNNNTNKHPSNDDYSSIFDISDVIALDPEIL 503

Query: 489 SITRSDAPLGNSTPATSSADLMQSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQNLQVQ 548
           S       LGN+ P                                             Q
Sbjct: 504 S------ALGNTAPQPH------------------------------------------Q 563

Query: 549 ALNSNENNQYGRMTLIPRPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQVGHGLNT 608
           A N+    QY  ++ IP  +   PV V   P     S +     TST+ + P        
Sbjct: 564 ASNTGTGQQYSNLSQIPMSIDPMPVPV---PFSQTPSPRDRPATTSTVFTIPNP------ 623

Query: 609 ITRDSERRLQFPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQASTPLRPSLG 668
                                                      +P  +   ++P+ P+  
Sbjct: 624 -------------------------------------------SPQYSQVHASPVTPTGT 683

Query: 669 LLTEFQNPHLQQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAYATVTSSQC 728
            L    +P              NQ   +  P + +P T  +V            VTS   
Sbjct: 684 YLGRTTSPRW------------NQTYQSQAPPMTTPYTSRKVS---------VPVTSQSP 725

Query: 729 ARM--MVTSQRA-EMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVGGMSQSV 788
           A +   V SQ    +L Q     +   TS  A + +  P G    +   + ++  +  +V
Sbjct: 744 ANVSSFVQSQHVPRVLSQPNNYGVRGLTSSHASTSRQHPSGPTVQSVSRLSDLVDVDLTV 725

Query: 789 PTSAGLVEPSLEQNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQ-SARPSSNPTPTPP 842
           P ++         NW+P  RMRGSL   ++S AL  ++I+P+Q  Q S R +S+     P
Sbjct: 804 PDTS---------NWRP--RMRGSLVPGSHSTALDHMIIRPSQQSQTSTRLNSSQPVQTP 725

BLAST of Moc06g33130 vs. TAIR 10
Match: AT1G08910.1 (zinc ion binding;zinc ion binding )

HSP 1 Score: 312.4 bits (799), Expect = 1.1e-84
Identity = 263/830 (31.69%), Postives = 397/830 (47.83%), Query Frame = 0

Query: 30  QFDPGQFYSICISLARSIDLSIANNKVPSQAHSLPGLLKQICQKK-HTHQTKAAIMVLMI 89
           +F+  +F + CISLA  ID +I  N+VP     L  +L  +C++K   +QT+A +M LMI
Sbjct: 14  EFNTKEFQASCISLANEIDAAIGRNEVPGNIQELALILNNVCRRKCDDYQTRAVVMALMI 73

Query: 90  SVKSACKMRWFSEKEAEELYSLANEIGSDFFGDVN-TGQTNS-LTTITTVMERFFPRMKL 149
           SVKSAC++ WF E+E +EL ++ + + + F    N T   NS +T I+ V+ERF+P +KL
Sbjct: 74  SVKSACQLGWFPERETQELLAIIDLMWNGFSCPENVTSCVNSPVTLISQVIERFYPCVKL 133

Query: 150 GQIIASVEVKPGYGVFATDFNISKTTQYSQQEKILLFVVQKDNIETSACLITPPQVNFLV 209
           G I+ S E KP   +   DF+ISK   +S ++K+ LFVV+ ++I  S C++ P  V+FL+
Sbjct: 134 GHILVSFEAKPESKMMMKDFHISKKMPHSPKQKVGLFVVRTEDISRSNCIVHPQGVSFLL 193

Query: 210 NGRGVNGRTNTGYTDTGPQLPTNIACMLKLGSNLLQAVGNFNGHYAIAVAIIGTAPSPDS 269
           NG+G++ R N    ++GPQLPTN+  +L LG+NLLQA+G F G Y IA+A +   P P+ 
Sbjct: 194 NGKGIDKRVNIS-MESGPQLPTNVTALLNLGANLLQAIGCFGGSYLIAIAFMDVIPLPNK 253

Query: 270 SMLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFID 329
            +L+D+V P V   +SD DIIEGPSRISL+CPIS TRIK+PVKG  CKHLQCFDF N+++
Sbjct: 254 PLLKDYVHPEVVGSNSDCDIIEGPSRISLSCPISRTRIKLPVKGHVCKHLQCFDFWNYVN 313

Query: 330 INSRRPSWRCPHCNQYICYLDIRVDQNMLKVLREVGENVTEVIISADGSWKAILNDYGDG 389
           +N+RR                 R+      +L EVG N  +V+ISADG+W  +  +  + 
Sbjct: 314 MNTRRHHGAA------------RI------ILEEVGRNAADVVISADGTW-MVETENDED 373

Query: 390 RPLEDSLKNQNGGAQQESTAPPDVLDLTEVDDNMNICNLETEDRKPCLSNKNQPVSSSLN 449
             L     + +G         P V +    D+N    + + E+  PCLS    P + +  
Sbjct: 374 VELVPETTHDHGDPNSFINLGPTVKNPAR-DENEMETSTQVEEHNPCLSEIQGPSNDTHR 433

Query: 450 ISSG---MNRNSLNQNFAAVLEDDFWSGIDETLTS----ITRSDAPLGNSTPATSSADLM 509
            +S    +N++  + N    L     +   +   +    I   D+P   + P T S    
Sbjct: 434 PASDYTMLNQSHTSTNTLPQLPRTLNAFDGQQFVNLPQVINTRDSPASQALPMTFSPTPS 493

Query: 510 QSAVLTEAVAPVLNHGVGVPGHAAFSSPALHDQNL-----QVQALNSNENNQYGRMTLIP 569
              +L    A   N G  +P   +      H  +L     +   L +  N+ YGR+    
Sbjct: 494 PQDILATNAA---NFGTSMPAAQSSQFQGSHVTSLGNCEGRTSDLMARWNHIYGRVQTQF 553

Query: 570 RPVSRTPVTVQALPAQSQGSGQLYSLRTSTISSAPQ---VGHGLNTITRDSERRL----Q 629
            P    P++      Q+Q           +  + PQ   V +G N   R     +     
Sbjct: 554 PP---APLSHHHYSMQNQSPSPAQQRPVPSYIAHPQTFHVNYGENADQRWMPSSIAHPQT 613

Query: 630 FPIHHGDPHRATNLAPFQRPPTVQIRDPQDRSFTPGLTVQASTPLRPSLGLLTEFQNPHL 689
            P+++G     TN    QRP    I  PQ    T  +  + +T  R      T +   HL
Sbjct: 614 LPVNYGG---NTN----QRPIPSSIAHPQ----TLPVNYRGNTDHRS-----TPYSITHL 673

Query: 690 QQALNMRMPQLRNQIPNNVRPSLGSPRTMNQVGGGGYGGAAYATVTSSQCARMMVTSQRA 749
           Q  LN      +  +P+++      P T        YGG A+    SS      +T  R 
Sbjct: 674 QTLLNYGGNADQRPMPSSITNLQTLPAT--------YGGYAHQRPMSSS-----ITHPRT 733

Query: 750 EMLKQSAAMSLLNQTSRSAHSLQTTPDGHRTAAAGEVRNVGG-MSQSVPTSAGLVEPSLE 809
             +            S   H  QT P  +      ++ N GG M Q        + P+  
Sbjct: 734 SPVNYGGTPDQRPMPSSITHP-QTLPVSY-GGTTDQILNPGGAMGQFSSREFMNLTPANT 779

Query: 810 QNWQPTGRMRGSLTGRTYSDALGQLLIQPTQSVQSARPSSNPTPTPPSTA 837
           +NW+P  RMRGS+   T  D    ++I PT+ V    P +   P P ST+
Sbjct: 794 ENWRPQSRMRGSVAPGTGYD---HMIIHPTRPV---HPQAQTPPAPLSTS 779

BLAST of Moc06g33130 vs. TAIR 10
Match: AT5G60410.2 (DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain )

HSP 1 Score: 77.8 bits (190), Expect = 4.7e-14
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 0

Query: 281 DSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCN 340
           DSD +++     ++L CP+S +RIKV  +   C H+ CFD D F+++N R   W+CP C 
Sbjct: 347 DSDIEVVADFFGVNLRCPMSGSRIKVAGRFLPCVHMGCFDLDVFVELNQRSRKWQCPICL 406

Query: 341 QYICYLDIRVDQNMLKV---LREVGENVTEVIISADGSWKAILNDYGDGRPL 390
           +      + VD    ++   ++   E VTE+ +  DGSW+       + R L
Sbjct: 407 KNYSVEHVIVDPYFNRITSKMKHCDEEVTEIEVKPDGSWRVKFKRESERREL 458

BLAST of Moc06g33130 vs. TAIR 10
Match: AT5G60410.1 (DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain )

HSP 1 Score: 77.8 bits (190), Expect = 4.7e-14
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 0

Query: 281 DSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCN 340
           DSD +++     ++L CP+S +RIKV  +   C H+ CFD D F+++N R   W+CP C 
Sbjct: 347 DSDIEVVADFFGVNLRCPMSGSRIKVAGRFLPCVHMGCFDLDVFVELNQRSRKWQCPICL 406

Query: 341 QYICYLDIRVDQNMLKV---LREVGENVTEVIISADGSWKAILNDYGDGRPL 390
           +      + VD    ++   ++   E VTE+ +  DGSW+       + R L
Sbjct: 407 KNYSVEHVIVDPYFNRITSKMKHCDEEVTEIEVKPDGSWRVKFKRESERREL 458

BLAST of Moc06g33130 vs. TAIR 10
Match: AT5G60410.3 (DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain )

HSP 1 Score: 77.8 bits (190), Expect = 4.7e-14
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 0

Query: 281 DSDSDIIEGPSRISLNCPISYTRIKVPVKGCSCKHLQCFDFDNFIDINSRRPSWRCPHCN 340
           DSD +++     ++L CP+S +RIKV  +   C H+ CFD D F+++N R   W+CP C 
Sbjct: 347 DSDIEVVADFFGVNLRCPMSGSRIKVAGRFLPCVHMGCFDLDVFVELNQRSRKWQCPICL 406

Query: 341 QYICYLDIRVDQNMLKV---LREVGENVTEVIISADGSWKAILNDYGDGRPL 390
           +      + VD    ++   ++   E VTE+ +  DGSW+       + R L
Sbjct: 407 KNYSVEHVIVDPYFNRITSKMKHCDEEVTEIEVKPDGSWRVKFKRESERREL 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6579533.10.0e+0074.10E4 SUMO-protein ligase PIAL2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022928990.10.0e+0073.76E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita moschata][more]
XP_022969988.10.0e+0073.41E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita maxima][more]
XP_023550945.10.0e+0072.83E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG7016993.10.0e+0071.77E4 SUMO-protein ligase PIAL2 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
F4JYG03.7e-11233.84E4 SUMO-protein ligase PIAL2 OS=Arabidopsis thaliana OX=3702 GN=PIAL2 PE=1 SV=1[more]
A0A0A7EPL02.6e-10233.86E4 SUMO-protein ligase PIAL1 OS=Arabidopsis thaliana OX=3702 GN=PIAL1 PE=2 SV=1[more]
O944511.9e-2023.71E3 SUMO-protein ligase pli1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... [more]
Q041952.4e-1831.84E3 SUMO-protein ligase SIZ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Q122162.4e-1838.71E3 SUMO-protein ligase SIZ2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Match NameE-valueIdentityDescription
A0A6J1ESZ60.0e+0073.76E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1I2J00.0e+0073.41E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EMF70.0e+0071.69E4 SUMO-protein ligase PIAL2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1K8Y80.0e+0071.63E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1DSP00.0e+00100.00E4 SUMO-protein ligase PIAL1-like OS=Momordica charantia OX=3673 GN=LOC111023949... [more]
Match NameE-valueIdentityDescription
AT5G41580.12.6e-11333.84RING/U-box superfamily protein [more]
AT1G08910.11.1e-8431.69zinc ion binding;zinc ion binding [more]
AT5G60410.24.7e-1432.14DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain [more]
AT5G60410.14.7e-1432.14DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain [more]
AT5G60410.34.7e-1432.14DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004181Zinc finger, MIZ-typePFAMPF02891zf-MIZcoord: 293..341
e-value: 3.6E-20
score: 71.4
IPR004181Zinc finger, MIZ-typePROSITEPS51044ZF_SP_RINGcoord: 282..359
score: 40.827038
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 262..378
e-value: 1.2E-38
score: 133.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 832..854
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 815..854
NoneNo IPR availablePANTHERPTHR10782:SF84E4 SUMO-PROTEIN LIGASE PIAL2-LIKEcoord: 18..744
NoneNo IPR availablePANTHERPTHR10782ZINC FINGER MIZ DOMAIN-CONTAINING PROTEINcoord: 18..744
NoneNo IPR availableCDDcd16650SP-RING_PIAS_likecoord: 294..341
e-value: 5.04844E-25
score: 96.1741

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g33130.1Moc06g33130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016925 protein sumoylation
molecular_function GO:0016874 ligase activity
molecular_function GO:0019789 SUMO transferase activity
molecular_function GO:0008270 zinc ion binding