Moc06g32340 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g32340
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptiondof zinc finger protein DOF4.6-like
Locationchr6: 24334568 .. 24335387 (-)
RNA-Seq ExpressionMoc06g32340
SyntenyMoc06g32340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACGCCCCATTGGCCACAGGTACTACACTCACAAATCTCTTCTCTCTCTCTCTCTCTCTGCTCTCTCTCTCTCTCTCTCTTCTCTGCAGTTCCATCAGAGAGAGAACTGCTTTTTTGTTTCCTTTTTAATTTCATTTGGGTAATTGTACTTATAAGTGGCGAAATGGGTATTGAATTGTGGACAGATGGAAGGAGCAGGAGCAGGAGGTTCAAAGGGTAATTTGTTGGAGAGAAAAGCTCGGCCTGCGAAGGAGGAAGCACTGAAATGTCCACGCTGCAATTCCAGCAACACCAAATTCTGTTACTACAATAATTACAGCCTCTCTCAGCCACGCTATTTCTGTAAGGCCTGTCGCAGGTACTGGACCGACGGCGGCTCCCTCAGAAATGTTCCTGTGGGCGGCGGCTCACGCAAGAACAAAATCAGGTCTGATCACAAGATCAAGATCAACGGCTCCGATCAAAACCCTAAAATCCACCATACCCAGGATCTCAATCTCGCCATCCTCCCTTCTTCAACTTCTTCTTCCTCTTCTTCTTCTCACAGCTTGCTGCTGGGTTCTTTCATGGCCGCGCCCAGCGCCGGCCTGGGGTTTTCGCTGGAGGGGTTTGAAAATAGTGGGTATGGGAATCTTCATCATCATCATCATCAACATCAACATCAACATCAAGATCAGACGGCTGCCATGTTTCCAATTGAGGATTTGAAGCAACCCAATGGAGCAGCGGCAGCAGCAGGGGCTGGGTTTGAGCATGGAGGAGGGGAATCAGGCGGTGGTTTTTGGAATGGGATGTTGGGAGGTGGATCATGGTAA

mRNA sequence

ATGGATACGCCCCATTGGCCACAGATGGAAGGAGCAGGAGCAGGAGGTTCAAAGGGTAATTTGTTGGAGAGAAAAGCTCGGCCTGCGAAGGAGGAAGCACTGAAATGTCCACGCTGCAATTCCAGCAACACCAAATTCTGTTACTACAATAATTACAGCCTCTCTCAGCCACGCTATTTCTGTAAGGCCTGTCGCAGGTACTGGACCGACGGCGGCTCCCTCAGAAATGTTCCTGTGGGCGGCGGCTCACGCAAGAACAAAATCAGGTCTGATCACAAGATCAAGATCAACGGCTCCGATCAAAACCCTAAAATCCACCATACCCAGGATCTCAATCTCGCCATCCTCCCTTCTTCAACTTCTTCTTCCTCTTCTTCTTCTCACAGCTTGCTGCTGGGTTCTTTCATGGCCGCGCCCAGCGCCGGCCTGGGGTTTTCGCTGGAGGGGTTTGAAAATAGTGGGTATGGGAATCTTCATCATCATCATCATCAACATCAACATCAACATCAAGATCAGACGGCTGCCATGTTTCCAATTGAGGATTTGAAGCAACCCAATGGAGCAGCGGCAGCAGCAGGGGCTGGGTTTGAGCATGGAGGAGGGGAATCAGGCGGTGGTTTTTGGAATGGGATGTTGGGAGGTGGATCATGGTAA

Coding sequence (CDS)

ATGGATACGCCCCATTGGCCACAGATGGAAGGAGCAGGAGCAGGAGGTTCAAAGGGTAATTTGTTGGAGAGAAAAGCTCGGCCTGCGAAGGAGGAAGCACTGAAATGTCCACGCTGCAATTCCAGCAACACCAAATTCTGTTACTACAATAATTACAGCCTCTCTCAGCCACGCTATTTCTGTAAGGCCTGTCGCAGGTACTGGACCGACGGCGGCTCCCTCAGAAATGTTCCTGTGGGCGGCGGCTCACGCAAGAACAAAATCAGGTCTGATCACAAGATCAAGATCAACGGCTCCGATCAAAACCCTAAAATCCACCATACCCAGGATCTCAATCTCGCCATCCTCCCTTCTTCAACTTCTTCTTCCTCTTCTTCTTCTCACAGCTTGCTGCTGGGTTCTTTCATGGCCGCGCCCAGCGCCGGCCTGGGGTTTTCGCTGGAGGGGTTTGAAAATAGTGGGTATGGGAATCTTCATCATCATCATCATCAACATCAACATCAACATCAAGATCAGACGGCTGCCATGTTTCCAATTGAGGATTTGAAGCAACCCAATGGAGCAGCGGCAGCAGCAGGGGCTGGGTTTGAGCATGGAGGAGGGGAATCAGGCGGTGGTTTTTGGAATGGGATGTTGGGAGGTGGATCATGGTAA

Protein sequence

MDTPHWPQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSSTSSSSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFPIEDLKQPNGAAAAAGAGFEHGGGESGGGFWNGMLGGGSW
Homology
BLAST of Moc06g32340 vs. NCBI nr
Match: XP_022138111.1 (dof zinc finger protein DOF4.6-like [Momordica charantia])

HSP 1 Score: 436.8 bits (1122), Expect = 1.1e-118
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 1   MDTPHWPQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYF 60
           MDTPHWPQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYF
Sbjct: 1   MDTPHWPQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYF 60

Query: 61  CKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST 120
           CKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST
Sbjct: 61  CKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST 120

Query: 121 SSSSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFPIE 180
           SSSSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFPIE
Sbjct: 121 SSSSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFPIE 180

Query: 181 DLKQPNGAAAAAGAGFEHGGGESGGGFWNGMLGGGSW 218
           DLKQPNGAAAAAGAGFEHGGGESGGGFWNGMLGGGSW
Sbjct: 181 DLKQPNGAAAAAGAGFEHGGGESGGGFWNGMLGGGSW 217

BLAST of Moc06g32340 vs. NCBI nr
Match: XP_038906804.1 (dof zinc finger protein DOF4.6-like [Benincasa hispida])

HSP 1 Score: 261.2 bits (666), Expect = 8.2e-66
Identity = 162/279 (58.06%), Postives = 173/279 (62.01%), Query Frame = 0

Query: 1   MDTPHWP----------QMEGAGAGGS----------------KGNLLERKARPAKEEAL 60
           MDT HWP          QMEG G+  S                 GNLLERKARP KE+AL
Sbjct: 1   MDTAHWPQFAGGGVVVKQMEGGGSSSSSKPNNNNNNNNNNNNNNGNLLERKARPQKEQAL 60

Query: 61  KCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNKIRS---- 120
            CPRCNS+NTKFCYYNNYSLSQPRYFCKACRRYWT+GGSLRNVPVGGGSRKNK  S    
Sbjct: 61  NCPRCNSTNTKFCYYNNYSLSQPRYFCKACRRYWTEGGSLRNVPVGGGSRKNKRSSSLSS 120

Query: 121 ------DHKIKINGSDQNPK-IHH--TQDLNLAILPSSTSS----SSSSSHSLLLGSF-- 180
                 DH  KIN SDQNPK IHH   QDLNLAI P ++++    SSSS+ S LLGSF  
Sbjct: 121 SSSAPLDHNKKINASDQNPKMIHHHAHQDLNLAIFPPTSNNPPIPSSSSTSSHLLGSFMT 180

Query: 181 MAAPSAG-----------------LGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFP 218
           MAAP+ G                 L FSLEGFEN G    HHHHH H H HQDQTA MFP
Sbjct: 181 MAAPAPGVFNAGGGFGLNELKPPTLSFSLEGFENGGGYGDHHHHHHHHHHHQDQTAVMFP 240

BLAST of Moc06g32340 vs. NCBI nr
Match: KAG6579311.1 (Dof zinc finger protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 254.2 bits (648), Expect = 1.0e-63
Identity = 161/284 (56.69%), Postives = 176/284 (61.97%), Query Frame = 0

Query: 1   MDTPHWPQMEGAGA------GG------------------------SKGNLLERKARPAK 60
           MDTP WPQ  G G       GG                        + GNLLERKARP K
Sbjct: 1   MDTPPWPQFAGGGVVVKQMEGGESSSSSKAGNININNVNININNNNNNGNLLERKARPQK 60

Query: 61  EEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNK--- 120
           E+AL CPRCNS+NTKFCYYNNYSLSQPRYFCK+CRRYWT+GGSLRNVPVGGGSRKNK   
Sbjct: 61  EQALNCPRCNSTNTKFCYYNNYSLSQPRYFCKSCRRYWTEGGSLRNVPVGGGSRKNKRSS 120

Query: 121 IRSDHKIKINGSDQNPK-IHHT-QDLNLAILP-------SSTSSSSSSSHSLLLGSFMAA 180
           + +    KINGSDQNPK IHH  QDLNLAI P       SS+SS++SSSH  L GSFMA 
Sbjct: 121 LSAPVDKKINGSDQNPKMIHHAHQDLNLAIFPPNNNNPISSSSSTTSSSH--LFGSFMAP 180

Query: 181 P----------------------SAGLGFSLEGFEN-SGYGNLHHHHHQHQHQHQDQTAA 218
           P                       + L FSL+GFE+  GYGN  HHHH HQ  HQDQTA 
Sbjct: 181 PPPPAAAAGVFNGAGGFGFNELKPSSLSFSLDGFESGGGYGN--HHHHHHQQDHQDQTAV 240

BLAST of Moc06g32340 vs. NCBI nr
Match: KAG7016811.1 (Dof zinc finger protein DOF4.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 254.2 bits (648), Expect = 1.0e-63
Identity = 161/284 (56.69%), Postives = 176/284 (61.97%), Query Frame = 0

Query: 1   MDTPHWPQMEGAGA------GG------------------------SKGNLLERKARPAK 60
           MDTP WPQ  G G       GG                        + GNLLERKARP K
Sbjct: 1   MDTPPWPQFAGGGVVVKQMEGGESSSSSKAGNININNVNININNNNNNGNLLERKARPQK 60

Query: 61  EEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNK--- 120
           E+AL CPRCNS+NTKFCYYNNYSLSQPRYFCK+CRRYWT+GGSLRNVPVGGGSRKNK   
Sbjct: 61  EQALNCPRCNSTNTKFCYYNNYSLSQPRYFCKSCRRYWTEGGSLRNVPVGGGSRKNKRSS 120

Query: 121 IRSDHKIKINGSDQNPK-IHHT-QDLNLAILP-------SSTSSSSSSSHSLLLGSFMAA 180
           + +    KINGSDQNPK IHH  QDLNLAI P       SS+SS++SSSH  L GSFMA 
Sbjct: 121 LSAPVDKKINGSDQNPKMIHHAHQDLNLAIFPPNNNNPISSSSSTTSSSH--LFGSFMAP 180

Query: 181 P----------------------SAGLGFSLEGFEN-SGYGNLHHHHHQHQHQHQDQTAA 218
           P                       + L FSL+GFE+  GYGN HHHHHQ    HQDQTA 
Sbjct: 181 PPPPAAAAGVFNGAGGFGFNELKPSSLSFSLDGFESGGGYGNHHHHHHQ---DHQDQTAV 240

BLAST of Moc06g32340 vs. NCBI nr
Match: XP_022922398.1 (dof zinc finger protein DOF4.6-like [Cucurbita moschata])

HSP 1 Score: 250.8 bits (639), Expect = 1.1e-62
Identity = 160/284 (56.34%), Postives = 175/284 (61.62%), Query Frame = 0

Query: 1   MDTPHWPQMEGAGA------GG------------------------SKGNLLERKARPAK 60
           MDTP WPQ  G G       GG                        + GNLLERKARP K
Sbjct: 1   MDTPPWPQFAGGGVVVKQMEGGESSSSSKAGNININNVNININNNNNNGNLLERKARPQK 60

Query: 61  EEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNK--- 120
           E+AL CPRCNS+NTKFCYYNNYSLSQPRYFCK+CRRYWT+GGSLRNVPVGGGSRKNK   
Sbjct: 61  EQALNCPRCNSTNTKFCYYNNYSLSQPRYFCKSCRRYWTEGGSLRNVPVGGGSRKNKRSS 120

Query: 121 IRSDHKIKINGSDQNPK-IHHT-QDLNLAILP-------SSTSSSSSSSHSLLLGSFMAA 180
           + +    KINGSDQNPK IHH  QDLNLAI P       SS+SS++SSSH  L GSFMA 
Sbjct: 121 LSAPVDKKINGSDQNPKMIHHAHQDLNLAIFPPNNNNPISSSSSTTSSSH--LFGSFMAP 180

Query: 181 P----------------------SAGLGFSLEGFEN-SGYGNLHHHHHQHQHQHQDQTAA 218
           P                       + L FSL+GFE+  GYGN  HHHHQ    HQDQTA 
Sbjct: 181 PPPPAAAAGVFNGAGGFGFNELKPSSLSFSLDGFESGGGYGN--HHHHQDHQDHQDQTAV 240

BLAST of Moc06g32340 vs. ExPASy Swiss-Prot
Match: Q9SXG8 (Dof zinc finger protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF1 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.8e-30
Identity = 125/328 (38.11%), Postives = 146/328 (44.51%), Query Frame = 0

Query: 7   PQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRR 66
           P   GAGAG +     ER+ARP KE+AL CPRCNS+NTKFCYYNNYSL QPRYFCK CRR
Sbjct: 56  PVAGGAGAGST-----ERRARPQKEKALNCPRCNSTNTKFCYYNNYSLQQPRYFCKTCRR 115

Query: 67  YWTDGGSLRNVPVGGGSRKNKIRSDHKIKIN-------------------------GSDQ 126
           YWT+GGSLRNVPVGGGSRKNK  S      +                         G+ +
Sbjct: 116 YWTEGGSLRNVPVGGGSRKNKRSSSSAASASPASASTANSVVTSASMSMSMASTGGGASK 175

Query: 127 NPKIHH--TQDLNLAI--------------LPSSTSSS----------------SSSSHS 186
           NPK+ H   QDLNLA                PS  SSS                + S+  
Sbjct: 176 NPKLVHEGAQDLNLAFPHHGGLQAPGEFPAFPSLESSSVCNPGGPMGTNGRGGGALSAME 235

Query: 187 LL-------------------------LGSFMAAP------SAGLGFSLE------GFEN 217
           LL                         LG F A P       + LGFSL+      G   
Sbjct: 236 LLRSTGCYMPLQVPMQMPAEYATPGFALGEFRAPPPPPQSSQSLLGFSLDAHGSVGGPSA 295

BLAST of Moc06g32340 vs. ExPASy Swiss-Prot
Match: Q8LAP8 (Dof zinc finger protein DOF4.6 OS=Arabidopsis thaliana OX=3702 GN=DOF4.6 PE=2 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 7.0e-28
Identity = 108/302 (35.76%), Postives = 137/302 (45.36%), Query Frame = 0

Query: 23  ERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGG 82
           ERKARP K++A+ CPRCNS+NTKFCYYNNYSL+QPRYFCK CRRYWT+GGSLRN+PVGGG
Sbjct: 42  ERKARPEKDQAVNCPRCNSTNTKFCYYNNYSLTQPRYFCKGCRRYWTEGGSLRNIPVGGG 101

Query: 83  SRKNK--IRSDHKIKINGSD---------------------------QNPKIHHT--QDL 142
           SRKNK    S   I  N SD                           QNPK   T  QDL
Sbjct: 102 SRKNKRSHSSSSDISNNHSDSTQPATKKHLSDHHHHLMSMSQQGLTGQNPKFLETTQQDL 161

Query: 143 NLAILP----------------SSTSSSSSSSHSLLL----------------------- 202
           NL   P                ++T+ S+++++ L++                       
Sbjct: 162 NLGFSPHGMIRTNFTDLIHNIGNNTNKSNNNNNPLIVSSCSAMATSSLDLIRNNSNNGNS 221

Query: 203 --GSFMAAP-------SAG----------------LGFSLEGFENSGY-GNLHHHHHQHQ 218
              SFM  P       S G                LGFSL+   N+G+ G         +
Sbjct: 222 SNSSFMGFPVHNQDPASGGFSMQDHYKPCNTNTTLLGFSLDHHHNNGFHGGFQGGEEGGE 281

BLAST of Moc06g32340 vs. ExPASy Swiss-Prot
Match: O24463 (Dof zinc finger protein PBF OS=Zea mays OX=4577 GN=PBF PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.2e-27
Identity = 70/134 (52.24%), Postives = 86/134 (64.18%), Query Frame = 0

Query: 7   PQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRR 66
           PQ+ G+G         ERK RP   EALKCPRC+S+NTKFCYYNNYS+SQPRYFCKACRR
Sbjct: 42  PQIGGSG---------ERKPRPQLPEALKCPRCDSNNTKFCYYNNYSMSQPRYFCKACRR 101

Query: 67  YWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSSTSSSSSS 126
           YWT GG+LRNVP+GGG RKNK  S     + GS      H +   +    P S S+++SS
Sbjct: 102 YWTHGGTLRNVPIGGGCRKNKHASRF---VLGS------HTSSSSSATYAPLSPSTNASS 157

Query: 127 SHSLLLGSFMAAPS 141
           S+  +    M  P+
Sbjct: 162 SNMSINKHMMMVPN 157

BLAST of Moc06g32340 vs. ExPASy Swiss-Prot
Match: Q84JQ8 (Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.6e-27
Identity = 79/191 (41.36%), Postives = 97/191 (50.79%), Query Frame = 0

Query: 1   MDTPHWPQ---------------MEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTK 60
           MDT  WPQ               ++      S    +ERKARP K++AL CPRCNS NTK
Sbjct: 1   MDTAKWPQEFVVKPMNEIVTNTCLKQQSNPPSPATPVERKARPEKDQALNCPRCNSLNTK 60

Query: 61  FCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHK------------ 120
           FCYYNNYSL+QPRYFCK CRRYWT GGSLRN+PVGGG RKNK  S +             
Sbjct: 61  FCYYNNYSLTQPRYFCKDCRRYWTAGGSLRNIPVGGGVRKNKRSSSNSSSSSPSSSSSSK 120

Query: 121 --IKINGSDQNPKIHHTQDLNLAILPSSTSSSSSSSHSLLLGSFMAAPSAGLGFSLEGFE 163
             +  N +   P + H   LN  I  ++ +     + S   G+        L FS     
Sbjct: 121 KPLFANNNTPTPPLPH---LNPKIGEAAATKVQDLTFSQGFGNAHEVKDLNLAFS----- 180

BLAST of Moc06g32340 vs. ExPASy Swiss-Prot
Match: B9F1L8 (Dof zinc finger protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF2 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.3e-26
Identity = 91/203 (44.83%), Postives = 111/203 (54.68%), Query Frame = 0

Query: 11  GAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTD 70
           GAGA G+ G   ER+ARP KE+AL CPRCNS+NTKFCYYNNYSL QPRYFCK CRRYWT+
Sbjct: 58  GAGAAGA-GAGTERRARPQKEKALNCPRCNSTNTKFCYYNNYSLQQPRYFCKTCRRYWTE 117

Query: 71  GGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSSTSSSSSSSHSL 130
           GGSLRNVPVGGGSRKNK  S                       +++PS+ +S+S+S+   
Sbjct: 118 GGSLRNVPVGGGSRKNKRSSS----------------------SVVPSAAASASTSA--- 177

Query: 131 LLGSFMAAPSAGLGFSLEGFENSGYGNLH---HHHHQHQHQHQDQTAAMFP---IEDLKQ 190
              +   +   GL        + G  +L+    HHH    Q  + TA  FP      +  
Sbjct: 178 ---AVSGSVPVGLAAKNPKLMHEGAQDLNLAFPHHHGRALQPPEFTA--FPSLESSSVCN 226

Query: 191 PNGAAAAAGAGFEHGGGESGGGF 208
           P G  AAA      GG  S G F
Sbjct: 238 PGGNLAAANGA---GGRGSVGAF 226

BLAST of Moc06g32340 vs. ExPASy TrEMBL
Match: A0A6J1C8S5 (dof zinc finger protein DOF4.6-like OS=Momordica charantia OX=3673 GN=LOC111009361 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 5.3e-119
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 1   MDTPHWPQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYF 60
           MDTPHWPQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYF
Sbjct: 1   MDTPHWPQMEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYF 60

Query: 61  CKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST 120
           CKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST
Sbjct: 61  CKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST 120

Query: 121 SSSSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFPIE 180
           SSSSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFPIE
Sbjct: 121 SSSSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQHQDQTAAMFPIE 180

Query: 181 DLKQPNGAAAAAGAGFEHGGGESGGGFWNGMLGGGSW 218
           DLKQPNGAAAAAGAGFEHGGGESGGGFWNGMLGGGSW
Sbjct: 181 DLKQPNGAAAAAGAGFEHGGGESGGGFWNGMLGGGSW 217

BLAST of Moc06g32340 vs. ExPASy TrEMBL
Match: A0A6J1E6H2 (dof zinc finger protein DOF4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111430405 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 5.4e-63
Identity = 160/284 (56.34%), Postives = 175/284 (61.62%), Query Frame = 0

Query: 1   MDTPHWPQMEGAGA------GG------------------------SKGNLLERKARPAK 60
           MDTP WPQ  G G       GG                        + GNLLERKARP K
Sbjct: 1   MDTPPWPQFAGGGVVVKQMEGGESSSSSKAGNININNVNININNNNNNGNLLERKARPQK 60

Query: 61  EEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNK--- 120
           E+AL CPRCNS+NTKFCYYNNYSLSQPRYFCK+CRRYWT+GGSLRNVPVGGGSRKNK   
Sbjct: 61  EQALNCPRCNSTNTKFCYYNNYSLSQPRYFCKSCRRYWTEGGSLRNVPVGGGSRKNKRSS 120

Query: 121 IRSDHKIKINGSDQNPK-IHHT-QDLNLAILP-------SSTSSSSSSSHSLLLGSFMAA 180
           + +    KINGSDQNPK IHH  QDLNLAI P       SS+SS++SSSH  L GSFMA 
Sbjct: 121 LSAPVDKKINGSDQNPKMIHHAHQDLNLAIFPPNNNNPISSSSSTTSSSH--LFGSFMAP 180

Query: 181 P----------------------SAGLGFSLEGFEN-SGYGNLHHHHHQHQHQHQDQTAA 218
           P                       + L FSL+GFE+  GYGN  HHHHQ    HQDQTA 
Sbjct: 181 PPPPAAAAGVFNGAGGFGFNELKPSSLSFSLDGFESGGGYGN--HHHHQDHQDHQDQTAV 240

BLAST of Moc06g32340 vs. ExPASy TrEMBL
Match: A0A6J1I6C6 (dof zinc finger protein DOF2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111471525 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 7.0e-63
Identity = 162/284 (57.04%), Postives = 176/284 (61.97%), Query Frame = 0

Query: 1   MDTPHWPQMEGAGA---------------GG---------------SKGNLLERKARPAK 60
           MDTP WPQ  G G                GG               + GNLLERKARP K
Sbjct: 1   MDTPPWPQFAGGGVVVKQMEGGESSSSSKGGNININNVNININNNNNNGNLLERKARPQK 60

Query: 61  EEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNK--- 120
           E+AL CPRCNS+NTKFCYYNNYSLSQPRYFCK+CRRYWT+GGSLRNVPVGGGSRKNK   
Sbjct: 61  EQALNCPRCNSTNTKFCYYNNYSLSQPRYFCKSCRRYWTEGGSLRNVPVGGGSRKNKRSS 120

Query: 121 IRSDHKIKINGSDQNPK-IHHT-QDLNLAILP-------SSTSSSSSSSHSLLLGSFMAA 180
           + +    KINGSDQNPK IHH  QDLNLAI P       SS+SS+SSSSH  L GSFMA 
Sbjct: 121 LSAPVDKKINGSDQNPKMIHHAHQDLNLAIFPPNNNNPISSSSSTSSSSH--LFGSFMAP 180

Query: 181 P----------------------SAGLGFSLEGFEN-SGYGNLHHHHHQHQHQHQDQTAA 218
           P                       + L FSL+GFE+ SGYGN HHHHHQ    HQDQTA 
Sbjct: 181 PPPPAAAAGVFNVAGGFGFNELKPSSLSFSLDGFESGSGYGN-HHHHHQ---DHQDQTAV 240

BLAST of Moc06g32340 vs. ExPASy TrEMBL
Match: A0A1S3AV39 (dof zinc finger protein DOF4.6-like OS=Cucumis melo OX=3656 GN=LOC103482981 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 2.6e-57
Identity = 153/283 (54.06%), Postives = 166/283 (58.66%), Query Frame = 0

Query: 1   MDTPHWP-----------QMEGAG-----------AGGSKGNLLERKARPAKEEALKCPR 60
           MDT HWP           QMEG G             G+  NLLERK R  KE+ALKCPR
Sbjct: 1   MDTAHWPQFAGGGGVVVKQMEGGGGSSSSCSKPNSGNGNGNNLLERKVRGEKEQALKCPR 60

Query: 61  CNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNK--------IRS 120
           CNSSNTKFCYYNNYSLSQPRYFCKACRRYWT+GGSLRNVPVGGGSRKNK          S
Sbjct: 61  CNSSNTKFCYYNNYSLSQPRYFCKACRRYWTEGGSLRNVPVGGGSRKNKRSSLSSSSTSS 120

Query: 121 DHKIKINGSDQNPKIHHTQDLNLAILP-------SSTSSSSSSSHSLLLGSFMAAPSAG- 180
           DH  KIN S Q   I + QDLNLAI P       ++TS S+SSS S LL SFM AP+ G 
Sbjct: 121 DHNKKINASHQ---IINHQDLNLAIFPPNNNNNNNNTSISTSSSSSHLLASFMTAPATGM 180

Query: 181 -----------------LGFSLEGFENS---GYGNLHHHHHQHQHQHQDQTAAMFPIEDL 218
                            L FSLEGF+ +   GYG+LHHHH      HQDQTA MFPIED+
Sbjct: 181 FNSSGGFGLNELKPPASLSFSLEGFDQNGVRGYGDLHHHH------HQDQTAVMFPIEDM 240

BLAST of Moc06g32340 vs. ExPASy TrEMBL
Match: A0A0A0KJS3 (Dof-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139210 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 5.4e-55
Identity = 149/270 (55.19%), Postives = 162/270 (60.00%), Query Frame = 0

Query: 8   QMEGAGAGGSK---------------GNLLERKARPAKEEALKCPRCNSSNTKFCYYNNY 67
           QMEG G GG                  NLLE+K R  KE+ALKCPRCNSSNTKFCYYNNY
Sbjct: 5   QMEGGGGGGGSSSSCSKPNSGNNGNGNNLLEKKVRGEKEQALKCPRCNSSNTKFCYYNNY 64

Query: 68  SLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNK--------IRSDHKIKINGSDQNPK 127
           SLSQPRYFCKACRRYWT+GGSLRNVPVGGGSRKNK          SDH  KIN S Q   
Sbjct: 65  SLSQPRYFCKACRRYWTEGGSLRNVPVGGGSRKNKRSSLSSSSSSSDHNKKINASHQ--- 124

Query: 128 IHHTQDLNLAILP------SSTSSSSSSSHSLLLGSFMAAPSAG---------------- 187
           I + QDLNLAI P      +++ S+SSSS S LL SFM AP+ G                
Sbjct: 125 IINHQDLNLAIFPPNNNNNNTSISTSSSSSSHLLASFMTAPATGMFNGSGGFGLNELKPP 184

Query: 188 --LGFSLEGFENS---GYGNLHHHHHQHQHQHQDQTAAMFPIEDLKQPNGAAAAAGAGFE 218
             L FSLEGF+ +   GYG+LHHHH      HQDQTA MFPIED+KQ N      G    
Sbjct: 185 ASLSFSLEGFDQNGVRGYGDLHHHH------HQDQTAVMFPIEDMKQSNDHEENRGG--- 244

BLAST of Moc06g32340 vs. TAIR 10
Match: AT4G24060.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 125.6 bits (314), Expect = 5.0e-29
Identity = 108/302 (35.76%), Postives = 137/302 (45.36%), Query Frame = 0

Query: 23  ERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGG 82
           ERKARP K++A+ CPRCNS+NTKFCYYNNYSL+QPRYFCK CRRYWT+GGSLRN+PVGGG
Sbjct: 42  ERKARPEKDQAVNCPRCNSTNTKFCYYNNYSLTQPRYFCKGCRRYWTEGGSLRNIPVGGG 101

Query: 83  SRKNK--IRSDHKIKINGSD---------------------------QNPKIHHT--QDL 142
           SRKNK    S   I  N SD                           QNPK   T  QDL
Sbjct: 102 SRKNKRSHSSSSDISNNHSDSTQPATKKHLSDHHHHLMSMSQQGLTGQNPKFLETTQQDL 161

Query: 143 NLAILP----------------SSTSSSSSSSHSLLL----------------------- 202
           NL   P                ++T+ S+++++ L++                       
Sbjct: 162 NLGFSPHGMIRTNFTDLIHNIGNNTNKSNNNNNPLIVSSCSAMATSSLDLIRNNSNNGNS 221

Query: 203 --GSFMAAP-------SAG----------------LGFSLEGFENSGY-GNLHHHHHQHQ 218
              SFM  P       S G                LGFSL+   N+G+ G         +
Sbjct: 222 SNSSFMGFPVHNQDPASGGFSMQDHYKPCNTNTTLLGFSLDHHHNNGFHGGFQGGEEGGE 281

BLAST of Moc06g32340 vs. TAIR 10
Match: AT3G61850.4 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-28
Identity = 73/123 (59.35%), Postives = 85/123 (69.11%), Query Frame = 0

Query: 13  GAGGSKGN---LLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKACRRYWT 72
           G+GG+  N   +  RKARP  +E + CPRCNS+NTKFCYYNNYSL+QPRYFCK CRRYWT
Sbjct: 52  GSGGNTNNTATMETRKARP--QEKVNCPRCNSTNTKFCYYNNYSLTQPRYFCKGCRRYWT 111

Query: 73  DGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSSTSSSSSSSHS 132
           +GGSLRNVPVGG SRKNK RS   +    S  NPK+    DLN  IL SS   + S+   
Sbjct: 112 EGGSLRNVPVGGSSRKNK-RSSTPL---ASPSNPKL---PDLNPPILFSSQIPNKSNKDL 165

BLAST of Moc06g32340 vs. TAIR 10
Match: AT1G64620.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 122.9 bits (307), Expect = 3.2e-28
Identity = 79/191 (41.36%), Postives = 97/191 (50.79%), Query Frame = 0

Query: 1   MDTPHWPQ---------------MEGAGAGGSKGNLLERKARPAKEEALKCPRCNSSNTK 60
           MDT  WPQ               ++      S    +ERKARP K++AL CPRCNS NTK
Sbjct: 1   MDTAKWPQEFVVKPMNEIVTNTCLKQQSNPPSPATPVERKARPEKDQALNCPRCNSLNTK 60

Query: 61  FCYYNNYSLSQPRYFCKACRRYWTDGGSLRNVPVGGGSRKNKIRSDHK------------ 120
           FCYYNNYSL+QPRYFCK CRRYWT GGSLRN+PVGGG RKNK  S +             
Sbjct: 61  FCYYNNYSLTQPRYFCKDCRRYWTAGGSLRNIPVGGGVRKNKRSSSNSSSSSPSSSSSSK 120

Query: 121 --IKINGSDQNPKIHHTQDLNLAILPSSTSSSSSSSHSLLLGSFMAAPSAGLGFSLEGFE 163
             +  N +   P + H   LN  I  ++ +     + S   G+        L FS     
Sbjct: 121 KPLFANNNTPTPPLPH---LNPKIGEAAATKVQDLTFSQGFGNAHEVKDLNLAFS----- 180

BLAST of Moc06g32340 vs. TAIR 10
Match: AT2G46590.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.4e-26
Identity = 82/166 (49.40%), Postives = 89/166 (53.61%), Query Frame = 0

Query: 11  GAGAGGSKGN------LLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKAC 70
           GA   G   N      + ERKARP  +E L CPRCNS+NTKFCYYNNYSL+QPRYFCK C
Sbjct: 41  GATVSGVSNNNNNTAVVAERKARP--QEKLNCPRCNSTNTKFCYYNNYSLTQPRYFCKGC 100

Query: 71  RRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST--SS 130
           RRYWT+GGSLRNVPVGG SRKNK RS      N     P      DLN  IL S+   + 
Sbjct: 101 RRYWTEGGSLRNVPVGGSSRKNK-RSSSSSSSNILQTIPS--SLPDLNPPILFSNQIHNK 160

Query: 131 SSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQ 169
           S  SS  L L SF                        HHHH H  Q
Sbjct: 161 SKGSSQDLNLLSFPVMQD------------------QHHHHVHMSQ 183

BLAST of Moc06g32340 vs. TAIR 10
Match: AT2G46590.2 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.4e-26
Identity = 82/166 (49.40%), Postives = 89/166 (53.61%), Query Frame = 0

Query: 11  GAGAGGSKGN------LLERKARPAKEEALKCPRCNSSNTKFCYYNNYSLSQPRYFCKAC 70
           GA   G   N      + ERKARP  +E L CPRCNS+NTKFCYYNNYSL+QPRYFCK C
Sbjct: 53  GATVSGVSNNNNNTAVVAERKARP--QEKLNCPRCNSTNTKFCYYNNYSLTQPRYFCKGC 112

Query: 71  RRYWTDGGSLRNVPVGGGSRKNKIRSDHKIKINGSDQNPKIHHTQDLNLAILPSST--SS 130
           RRYWT+GGSLRNVPVGG SRKNK RS      N     P      DLN  IL S+   + 
Sbjct: 113 RRYWTEGGSLRNVPVGGSSRKNK-RSSSSSSSNILQTIPS--SLPDLNPPILFSNQIHNK 172

Query: 131 SSSSSHSLLLGSFMAAPSAGLGFSLEGFENSGYGNLHHHHHQHQHQ 169
           S  SS  L L SF                        HHHH H  Q
Sbjct: 173 SKGSSQDLNLLSFPVMQD------------------QHHHHVHMSQ 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138111.11.1e-118100.00dof zinc finger protein DOF4.6-like [Momordica charantia][more]
XP_038906804.18.2e-6658.06dof zinc finger protein DOF4.6-like [Benincasa hispida][more]
KAG6579311.11.0e-6356.69Dof zinc finger protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7016811.11.0e-6356.69Dof zinc finger protein DOF4.6, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022922398.11.1e-6256.34dof zinc finger protein DOF4.6-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SXG85.8e-3038.11Dof zinc finger protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF1 PE=2 ... [more]
Q8LAP87.0e-2835.76Dof zinc finger protein DOF4.6 OS=Arabidopsis thaliana OX=3702 GN=DOF4.6 PE=2 SV... [more]
O244631.2e-2752.24Dof zinc finger protein PBF OS=Zea mays OX=4577 GN=PBF PE=1 SV=1[more]
Q84JQ84.6e-2741.36Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV... [more]
B9F1L82.3e-2644.83Dof zinc finger protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF2 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1C8S55.3e-119100.00dof zinc finger protein DOF4.6-like OS=Momordica charantia OX=3673 GN=LOC1110093... [more]
A0A6J1E6H25.4e-6356.34dof zinc finger protein DOF4.6-like OS=Cucurbita moschata OX=3662 GN=LOC11143040... [more]
A0A6J1I6C67.0e-6357.04dof zinc finger protein DOF2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111471525 ... [more]
A0A1S3AV392.6e-5754.06dof zinc finger protein DOF4.6-like OS=Cucumis melo OX=3656 GN=LOC103482981 PE=4... [more]
A0A0A0KJS35.4e-5555.19Dof-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139210 PE... [more]
Match NameE-valueIdentityDescription
AT4G24060.15.0e-2935.76Dof-type zinc finger DNA-binding family protein [more]
AT3G61850.41.1e-2859.35Dof-type zinc finger DNA-binding family protein [more]
AT1G64620.13.2e-2841.36Dof-type zinc finger DNA-binding family protein [more]
AT2G46590.11.4e-2649.40Dof-type zinc finger DNA-binding family protein [more]
AT2G46590.21.4e-2649.40Dof-type zinc finger DNA-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003851Zinc finger, Dof-typePFAMPF02701zf-Dofcoord: 32..87
e-value: 7.0E-34
score: 115.8
IPR003851Zinc finger, Dof-typePROSITEPS01361ZF_DOF_1coord: 36..72
IPR003851Zinc finger, Dof-typePROSITEPS50884ZF_DOF_2coord: 34..88
score: 28.900547
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR31992DOF ZINC FINGER PROTEIN DOF1.4-RELATEDcoord: 20..197
NoneNo IPR availablePANTHERPTHR31992:SF161DOF ZINC FINGER PROTEIN DOF3.5-LIKEcoord: 20..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g32340.1Moc06g32340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding