Moc06g31810 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc06g31810 vs. NCBI nr
Match: XP_022154803.1 (uncharacterized protein LOC111021969 [Momordica charantia])

HSP 1 Score: 245.4 bits (625), Expect = 6.8e-61
Identity = 155/382 (40.58%), Postives = 215/382 (56.28%), Query Frame = 0

Query: 5   FISYGDMWNESQSVYEGGIMRGLDVDDNITYDELISAMFMLTRIDRDQFKLLIRCVYLLK 64
           F      WNE+ +VYEGG+M GL+VD+ ITY +L+SA+F +TRI+ D F ++++C+Y  +
Sbjct: 12  FCKLWGQWNEAGTVYEGGVMGGLNVDEGITYRDLVSAIFRMTRINPDIFNIVLQCIYKFE 71

Query: 65  PQY----------------------------------QERHGSGSTSQN-HIVPKTKAFH 124
            QY                                  +ER  SGS S +  + P+T+ F 
Sbjct: 72  FQYGVPNFYIFDDTSLGFYLMGPPHPSQVPLYVSVVPKERQCSGSNSHDVPLYPQTETFA 131

Query: 125 SVSDQIGHYVP-------------------SVLLNSTLLDAGPSCNLDDDETNCCGQWDD 184
           S   Q+   VP                   SV   + L D    CNL DDE    GQWDD
Sbjct: 132 SFPWQVEQNVPSPAPLNHVHSSIRTVHPPSSVKFMTPLTDNVVPCNLGDDEHEHFGQWDD 191

Query: 185 --NDDNHV------------EYEENDADIENEHEVDLEDVAAPSLDPLIEVHMVSTNAPC 244
             +D++H             +Y+E +++ +NE +  + D       P++EV  VS NAPC
Sbjct: 192 VGDDEDHEYRMVGDDLEDDDDYQEIESEDDNEADEPVYDGDPSLRTPVVEVQSVSRNAPC 251

Query: 245 ET--GQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQFKLSVFAMRLNFEYRVKK 304
            T  G  +    +  IG D+++    +DIA+G+ FRSK+EL+F L+VFA++ NFE++VKK
Sbjct: 252 ATVEGVHASLEQMDTIGCDDDL---VDDIALGSLFRSKDELRFTLAVFAIQKNFEFQVKK 311

Query: 305 SNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCTREVMIHDHRQARSHVVGQ 317
           S +SL +V C E+GC+W LRAR IKGSDTFLISTF E H   RE + HDH+QA S VVGQ
Sbjct: 312 STRSLLSVACKEEGCRWALRARKIKGSDTFLISTFSEDHIHERETLRHDHKQAGSWVVGQ 371

BLAST of Moc06g31810 vs. NCBI nr
Match: XP_022158743.1 (PKS-NRPS hybrid synthetase CHGG_01239-like [Momordica charantia])

HSP 1 Score: 245.0 bits (624), Expect = 8.9e-61
Identity = 147/287 (51.22%), Postives = 182/287 (63.41%), Query Frame = 0

Query: 68  QERHGSGSTSQNHIVPKTKAFHSVSDQIGHYVP-SVLLNSTLLDAGPSC----------- 127
           ++RHG GS+S+N I P  +AFHS   Q+G  VP  + ++STL   G SC           
Sbjct: 4   KQRHGYGSSSKNPINPTCQAFHSFPYQLGQDVPIPIPIHSTLPGIGLSCSVLSITPLTDN 63

Query: 128 ----NLDDDETNCCGQWDDNDDN---HVEYEENDAD-------------------IENEH 187
               NL DDE N   QWD++DDN   + E E+ND D                   I+NE 
Sbjct: 64  VVSYNLGDDELNYHSQWDEDDDNDGYNDEAEDNDGDNEGGDKFEGDDAEFVNDVGIDNEV 123

Query: 188 EVDLEDVAAPSLDPLIEVHMVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTF 247
           EV+  DV A S  PL E++ VS NAPC T QASCSR +++  +  E+GL++E I V + F
Sbjct: 124 EVEPMDVYATSSAPLHEMNRVSMNAPCATNQASCSRDISKTVD--ELGLTSEKIVVWSAF 183

Query: 248 RSKEELQFKLSVFAMRLNFEYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTF 307
           +S  ELQF  SVFAM+LNFEYRVKKS KSL TVGC  DGCKW + AR I+GSDTFLIS F
Sbjct: 184 KSNVELQFNQSVFAMKLNFEYRVKKSTKSLLTVGCCGDGCKWGMCARRIQGSDTFLISKF 243

Query: 308 HELHSCTREVMIHDHRQARSHVVGQLIKSNLEDVSRCYRPNDIVNDM 317
             +H+C  EVM HDHRQARS +VGQ+IK+N ED SR YRP DIVN+M
Sbjct: 244 FGVHNCKDEVMKHDHRQARSRMVGQIIKTNFEDPSRRYRPKDIVNNM 288

BLAST of Moc06g31810 vs. NCBI nr
Match: XP_022156802.1 (uncharacterized protein LOC111023635 [Momordica charantia])

HSP 1 Score: 242.3 bits (617), Expect = 5.7e-60
Identity = 155/340 (45.59%), Postives = 193/340 (56.76%), Query Frame = 0

Query: 1   MTYLFISYGDMWNESQSVYEGGIMRGLDVDDNITYDELISAMFMLTRIDRDQFKLLIRCV 60
           M  LF+ YG  WNE+ +VYEGG M GLDVD+ ITY  L+SA+ MLTRID DQF L+++CV
Sbjct: 1   MRRLFVIYGGKWNEAGTVYEGGXMGGLDVDETITYANLVSALHMLTRIDLDQFDLIVQCV 60

Query: 61  YLLKPQYQERHGSGSTSQNHIVPKTKAFHSVSDQIGHYVPSVLLNSTLLDAGPSCNLDDD 120
           Y                               D I    P       L D   SCNLDDD
Sbjct: 61  Y-------------------------------DLIFEITP-------LADNVISCNLDDD 120

Query: 121 ET-NCCGQWDDND-------DNHVEYEE------------NDADIENEHEVDLEDVAAPS 180
           E  +  GQ  +N+       D+  +Y+E            N  D   E E   EDV    
Sbjct: 121 ENDDGNGQLYENEVEYEFQSDDEYDYDEYRTEDGVEDDGGNGYDNGEEDEYHTEDVLDEH 180

Query: 181 LD----PLIEVHMVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQ 240
            +     + E H VS NAP +T +    R +  I   N      E IAV   FRSKEEL+
Sbjct: 181 YENEGQHVHESHTVSGNAPYQTVEEVVXRPMQNIITGNGSDFIGE-IAVKGIFRSKEELR 240

Query: 241 FKLSVFAMRLNFEYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCT 300
           FKLSV AM+LNF+++VKKS K+L+TVGC E GCKW LRA++I+G D+F+IS F++ H C 
Sbjct: 241 FKLSVLAMKLNFKFKVKKSTKTLFTVGCTEQGCKWGLRAKSIQGGDSFIISKFNDFHKCK 300

Query: 301 REVMIHDHRQARSHVVGQLIKSNLEDVSRCYRPNDIVNDM 317
           REV+ HDHRQARS VVGQL+KSN+EDVSR YRP DI+NDM
Sbjct: 301 REVLNHDHRQARSWVVGQLVKSNVEDVSRQYRPKDIINDM 301

BLAST of Moc06g31810 vs. NCBI nr
Match: XP_022159183.1 (uncharacterized protein LOC111025603 [Momordica charantia])

HSP 1 Score: 215.3 bits (547), Expect = 7.5e-52
Identity = 109/148 (73.65%), Postives = 122/148 (82.43%), Query Frame = 0

Query: 169 MVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQFKLSVFAMRLNF 228
           MVSTNA C T QAS SR + R   D+EVGLS +DIA+G+TFRSKEELQFKLSVFAMR+NF
Sbjct: 1   MVSTNAWCATCQASWSREMAR--TDDEVGLSMKDIAIGSTFRSKEELQFKLSVFAMRVNF 60

Query: 229 EYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCTREVMIHDHRQAR 288
           EY VKKS KSLY +GC EDGCKW+   R I+GSD FLISTF+E+HSC REVM HDHRQA+
Sbjct: 61  EYHVKKSTKSLYPIGCSEDGCKWSPHVRKIQGSDIFLISTFYEVHSCIREVMKHDHRQAQ 120

Query: 289 SHVVGQLIKSNLEDVSRCYRPNDIVNDM 317
           S VVGQ+IKSN EDVS  YRP DIVNDM
Sbjct: 121 SRVVGQIIKSNFEDVSCRYRPKDIVNDM 146

BLAST of Moc06g31810 vs. NCBI nr
Match: XP_022156834.1 (uncharacterized protein LOC111023667 [Momordica charantia])

HSP 1 Score: 176.0 bits (445), Expect = 5.1e-40
Identity = 137/394 (34.77%), Postives = 184/394 (46.70%), Query Frame = 0

Query: 1   MTYLFISYGDMWNESQSVYEGGIMRGLDVDDNITYDELISAMFMLTRIDRDQFKLLIRCV 60
           M  LF+ YG  WNE+ ++YEGG+M GLDVD+ ITY  L+SA+ MLTRID DQF L+++CV
Sbjct: 21  MRRLFVIYGGKWNEAGTMYEGGVMGGLDVDETITYANLVSALHMLTRIDPDQFDLIVQCV 80

Query: 61  YLLKPQYQ----------------------------------ERHGSGSTSQNHIVP--- 120
           Y    +Y+                                    +GSGS   N+      
Sbjct: 81  YRFDFRYEVPNYLIFDDSSLKFYLNGPPDPSQVPLYVTVIPKASYGSGSRRGNNFFETQT 140

Query: 121 ----------------KTKAFHSVSDQIG--HYVPSVLLNSTLLDAGPSCNLDDDETNCC 180
                           +T +  S  D +G   ++P +   + L D    CNLDDDE+   
Sbjct: 141 SSSFPYYPRQNDPCFGRTISIDSPLDDVGLPSFIPQI---TPLADNVIPCNLDDDESPYY 200

Query: 181 GQWDDND-------DNHVEYEE------------NDADIENEHEVDLEDVAAPSLD---- 240
           GQ  +N+       D+  +Y+E            N  D   E E   EDV     +    
Sbjct: 201 GQLYENEVEYKFQSDDEYDYDEYRTEDGVEGDGGNGYDNGEEDEYHTEDVLDEHYENEGQ 260

Query: 241 PLIEVHMVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQFKLSVF 300
            + E H +S NAP +T +   SR +  I   N      E IAV   F SK EL+FKL   
Sbjct: 261 HVHESHTMSGNAPYQTVEEVVSRPMQNIITGNGSDFIGE-IAVKGIFHSKVELRFKL--- 320

Query: 301 AMRLNFEYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCTREVMIH 317
                                         LRA++I+G D+F+IS F+++H C REV+ H
Sbjct: 321 ------------------------------LRAKSIQGGDSFIISKFNDVHKCKREVLNH 377

BLAST of Moc06g31810 vs. ExPASy TrEMBL
Match: A0A6J1DLB0 (uncharacterized protein LOC111021969 OS=Momordica charantia OX=3673 GN=LOC111021969 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 3.3e-61
Identity = 155/382 (40.58%), Postives = 215/382 (56.28%), Query Frame = 0

Query: 5   FISYGDMWNESQSVYEGGIMRGLDVDDNITYDELISAMFMLTRIDRDQFKLLIRCVYLLK 64
           F      WNE+ +VYEGG+M GL+VD+ ITY +L+SA+F +TRI+ D F ++++C+Y  +
Sbjct: 12  FCKLWGQWNEAGTVYEGGVMGGLNVDEGITYRDLVSAIFRMTRINPDIFNIVLQCIYKFE 71

Query: 65  PQY----------------------------------QERHGSGSTSQN-HIVPKTKAFH 124
            QY                                  +ER  SGS S +  + P+T+ F 
Sbjct: 72  FQYGVPNFYIFDDTSLGFYLMGPPHPSQVPLYVSVVPKERQCSGSNSHDVPLYPQTETFA 131

Query: 125 SVSDQIGHYVP-------------------SVLLNSTLLDAGPSCNLDDDETNCCGQWDD 184
           S   Q+   VP                   SV   + L D    CNL DDE    GQWDD
Sbjct: 132 SFPWQVEQNVPSPAPLNHVHSSIRTVHPPSSVKFMTPLTDNVVPCNLGDDEHEHFGQWDD 191

Query: 185 --NDDNHV------------EYEENDADIENEHEVDLEDVAAPSLDPLIEVHMVSTNAPC 244
             +D++H             +Y+E +++ +NE +  + D       P++EV  VS NAPC
Sbjct: 192 VGDDEDHEYRMVGDDLEDDDDYQEIESEDDNEADEPVYDGDPSLRTPVVEVQSVSRNAPC 251

Query: 245 ET--GQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQFKLSVFAMRLNFEYRVKK 304
            T  G  +    +  IG D+++    +DIA+G+ FRSK+EL+F L+VFA++ NFE++VKK
Sbjct: 252 ATVEGVHASLEQMDTIGCDDDL---VDDIALGSLFRSKDELRFTLAVFAIQKNFEFQVKK 311

Query: 305 SNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCTREVMIHDHRQARSHVVGQ 317
           S +SL +V C E+GC+W LRAR IKGSDTFLISTF E H   RE + HDH+QA S VVGQ
Sbjct: 312 STRSLLSVACKEEGCRWALRARKIKGSDTFLISTFSEDHIHERETLRHDHKQAGSWVVGQ 371

BLAST of Moc06g31810 vs. ExPASy TrEMBL
Match: A0A6J1DWY9 (PKS-NRPS hybrid synthetase CHGG_01239-like OS=Momordica charantia OX=3673 GN=LOC111025208 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 4.3e-61
Identity = 147/287 (51.22%), Postives = 182/287 (63.41%), Query Frame = 0

Query: 68  QERHGSGSTSQNHIVPKTKAFHSVSDQIGHYVP-SVLLNSTLLDAGPSC----------- 127
           ++RHG GS+S+N I P  +AFHS   Q+G  VP  + ++STL   G SC           
Sbjct: 4   KQRHGYGSSSKNPINPTCQAFHSFPYQLGQDVPIPIPIHSTLPGIGLSCSVLSITPLTDN 63

Query: 128 ----NLDDDETNCCGQWDDNDDN---HVEYEENDAD-------------------IENEH 187
               NL DDE N   QWD++DDN   + E E+ND D                   I+NE 
Sbjct: 64  VVSYNLGDDELNYHSQWDEDDDNDGYNDEAEDNDGDNEGGDKFEGDDAEFVNDVGIDNEV 123

Query: 188 EVDLEDVAAPSLDPLIEVHMVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTF 247
           EV+  DV A S  PL E++ VS NAPC T QASCSR +++  +  E+GL++E I V + F
Sbjct: 124 EVEPMDVYATSSAPLHEMNRVSMNAPCATNQASCSRDISKTVD--ELGLTSEKIVVWSAF 183

Query: 248 RSKEELQFKLSVFAMRLNFEYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTF 307
           +S  ELQF  SVFAM+LNFEYRVKKS KSL TVGC  DGCKW + AR I+GSDTFLIS F
Sbjct: 184 KSNVELQFNQSVFAMKLNFEYRVKKSTKSLLTVGCCGDGCKWGMCARRIQGSDTFLISKF 243

Query: 308 HELHSCTREVMIHDHRQARSHVVGQLIKSNLEDVSRCYRPNDIVNDM 317
             +H+C  EVM HDHRQARS +VGQ+IK+N ED SR YRP DIVN+M
Sbjct: 244 FGVHNCKDEVMKHDHRQARSRMVGQIIKTNFEDPSRRYRPKDIVNNM 288

BLAST of Moc06g31810 vs. ExPASy TrEMBL
Match: A0A6J1DSY0 (uncharacterized protein LOC111023635 OS=Momordica charantia OX=3673 GN=LOC111023635 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 2.8e-60
Identity = 155/340 (45.59%), Postives = 193/340 (56.76%), Query Frame = 0

Query: 1   MTYLFISYGDMWNESQSVYEGGIMRGLDVDDNITYDELISAMFMLTRIDRDQFKLLIRCV 60
           M  LF+ YG  WNE+ +VYEGG M GLDVD+ ITY  L+SA+ MLTRID DQF L+++CV
Sbjct: 1   MRRLFVIYGGKWNEAGTVYEGGXMGGLDVDETITYANLVSALHMLTRIDLDQFDLIVQCV 60

Query: 61  YLLKPQYQERHGSGSTSQNHIVPKTKAFHSVSDQIGHYVPSVLLNSTLLDAGPSCNLDDD 120
           Y                               D I    P       L D   SCNLDDD
Sbjct: 61  Y-------------------------------DLIFEITP-------LADNVISCNLDDD 120

Query: 121 ET-NCCGQWDDND-------DNHVEYEE------------NDADIENEHEVDLEDVAAPS 180
           E  +  GQ  +N+       D+  +Y+E            N  D   E E   EDV    
Sbjct: 121 ENDDGNGQLYENEVEYEFQSDDEYDYDEYRTEDGVEDDGGNGYDNGEEDEYHTEDVLDEH 180

Query: 181 LD----PLIEVHMVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQ 240
            +     + E H VS NAP +T +    R +  I   N      E IAV   FRSKEEL+
Sbjct: 181 YENEGQHVHESHTVSGNAPYQTVEEVVXRPMQNIITGNGSDFIGE-IAVKGIFRSKEELR 240

Query: 241 FKLSVFAMRLNFEYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCT 300
           FKLSV AM+LNF+++VKKS K+L+TVGC E GCKW LRA++I+G D+F+IS F++ H C 
Sbjct: 241 FKLSVLAMKLNFKFKVKKSTKTLFTVGCTEQGCKWGLRAKSIQGGDSFIISKFNDFHKCK 300

Query: 301 REVMIHDHRQARSHVVGQLIKSNLEDVSRCYRPNDIVNDM 317
           REV+ HDHRQARS VVGQL+KSN+EDVSR YRP DI+NDM
Sbjct: 301 REVLNHDHRQARSWVVGQLVKSNVEDVSRQYRPKDIINDM 301

BLAST of Moc06g31810 vs. ExPASy TrEMBL
Match: A0A6J1DY41 (uncharacterized protein LOC111025603 OS=Momordica charantia OX=3673 GN=LOC111025603 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 3.6e-52
Identity = 109/148 (73.65%), Postives = 122/148 (82.43%), Query Frame = 0

Query: 169 MVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQFKLSVFAMRLNF 228
           MVSTNA C T QAS SR + R   D+EVGLS +DIA+G+TFRSKEELQFKLSVFAMR+NF
Sbjct: 1   MVSTNAWCATCQASWSREMAR--TDDEVGLSMKDIAIGSTFRSKEELQFKLSVFAMRVNF 60

Query: 229 EYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCTREVMIHDHRQAR 288
           EY VKKS KSLY +GC EDGCKW+   R I+GSD FLISTF+E+HSC REVM HDHRQA+
Sbjct: 61  EYHVKKSTKSLYPIGCSEDGCKWSPHVRKIQGSDIFLISTFYEVHSCIREVMKHDHRQAQ 120

Query: 289 SHVVGQLIKSNLEDVSRCYRPNDIVNDM 317
           S VVGQ+IKSN EDVS  YRP DIVNDM
Sbjct: 121 SRVVGQIIKSNFEDVSCRYRPKDIVNDM 146

BLAST of Moc06g31810 vs. ExPASy TrEMBL
Match: A0A6J1DUS4 (uncharacterized protein LOC111023667 OS=Momordica charantia OX=3673 GN=LOC111023667 PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 2.4e-40
Identity = 137/394 (34.77%), Postives = 184/394 (46.70%), Query Frame = 0

Query: 1   MTYLFISYGDMWNESQSVYEGGIMRGLDVDDNITYDELISAMFMLTRIDRDQFKLLIRCV 60
           M  LF+ YG  WNE+ ++YEGG+M GLDVD+ ITY  L+SA+ MLTRID DQF L+++CV
Sbjct: 21  MRRLFVIYGGKWNEAGTMYEGGVMGGLDVDETITYANLVSALHMLTRIDPDQFDLIVQCV 80

Query: 61  YLLKPQYQ----------------------------------ERHGSGSTSQNHIVP--- 120
           Y    +Y+                                    +GSGS   N+      
Sbjct: 81  YRFDFRYEVPNYLIFDDSSLKFYLNGPPDPSQVPLYVTVIPKASYGSGSRRGNNFFETQT 140

Query: 121 ----------------KTKAFHSVSDQIG--HYVPSVLLNSTLLDAGPSCNLDDDETNCC 180
                           +T +  S  D +G   ++P +   + L D    CNLDDDE+   
Sbjct: 141 SSSFPYYPRQNDPCFGRTISIDSPLDDVGLPSFIPQI---TPLADNVIPCNLDDDESPYY 200

Query: 181 GQWDDND-------DNHVEYEE------------NDADIENEHEVDLEDVAAPSLD---- 240
           GQ  +N+       D+  +Y+E            N  D   E E   EDV     +    
Sbjct: 201 GQLYENEVEYKFQSDDEYDYDEYRTEDGVEGDGGNGYDNGEEDEYHTEDVLDEHYENEGQ 260

Query: 241 PLIEVHMVSTNAPCETGQASCSRVLTRIGEDNEVGLSTEDIAVGTTFRSKEELQFKLSVF 300
            + E H +S NAP +T +   SR +  I   N      E IAV   F SK EL+FKL   
Sbjct: 261 HVHESHTMSGNAPYQTVEEVVSRPMQNIITGNGSDFIGE-IAVKGIFHSKVELRFKL--- 320

Query: 301 AMRLNFEYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGSDTFLISTFHELHSCTREVMIH 317
                                         LRA++I+G D+F+IS F+++H C REV+ H
Sbjct: 321 ------------------------------LRAKSIQGGDSFIISKFNDVHKCKREVLNH 377

BLAST of Moc06g31810 vs. TAIR 10
Match: AT1G64260.1 (MuDR family transposase )

HSP 1 Score: 45.8 bits (107), Expect = 7.3e-05
Identity = 21/77 (27.27%), Postives = 42/77 (54.55%), Query Frame = 0

Query: 202 DIAVGTTFRSKEELQFKLSVFAMRLNFEYRVKKSNKSLYTVGCLEDGCKWNLRARTIKGS 261
           D+ +G  F+ ++EL+  +  + +R      V+++ K +YT  C+   CKW+LRA  ++  
Sbjct: 184 DMHLGLCFKDRDELKKAVDWWCIRRRRNCIVRETEKEMYTFECVRWKCKWSLRAARMEEH 243

Query: 262 DTFLISTFHELHSCTRE 279
               I+ +   H+C+ E
Sbjct: 244 GLVEITKYTGPHTCSHE 260