Moc06g31220 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g31220
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrotrans_gag domain-containing protein
Locationchr6: 23489885 .. 23490787 (-)
RNA-Seq ExpressionMoc06g31220
SyntenyMoc06g31220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTGCAGAATGTAGAGCTAAAGAAGATGAGACAAGACATCGATAATATTCAGGAGCAAGTCATGGAAATTTTAAATGTCTTAAAGAATATTGTTACAGTTGGGGACATTTCTCAACCTACCAATTTAGTCGAATCTCATAATAATTCATCACCTAGGGTGGACCCACCTTTTTGTCAATCAAAAGAGGGTTTCCCTCAGCACAGTTCGTCACATGACGCTCAGCTTTCCAAGAAAGGCCAACAAGTTCCTACAAACTGCCAGGTTGAATTCACATTCAAAGATGGACAAACATCAAAGGTCAATTACTCAGATAATTTAAAAGGTAAAGAAAAAGAAAATGTTACCAATGGAAATGTTGAAGGAACTGCAGGGAAAGAATATGCTCAAAGATGGGGAGAAGCAACAGAACAGCTGTCGGTGCCTTTAATTAATAAAGAGCCGCATCCTGAAACGAATAGAAGCTCTATTAAAAGGAAAAATAAAGTCCAGGCAGTAATTTCGAAGCGCAAGGGGAAGTACCAACAAGGATTTGTGGCATACAGTCCTTCTACGGTACCCATAAACTCATTGACTCTGCAGAATGATCCACAAGCATTACCCGGTAATCATAGTAGTCGGAGGCAACTAAAGCGAGAGAGCTTCCATCCAATCCCAATATCCTACACTGAGTTGTTATCCCAATTATTTCAAGGTAATCTATTAGCTCCCGTATCCGTAGAGCCATTGCAACCACCTTATCCAAAGTGGTACGACCCGCAAGTCCACTATGATTACCATGCAGGACCTATATGTCATTCAACTGAGAACTGTACTTCACTAAAGCATAAAGTGCAAGTTCTAATGAAGGCAGGATGGCTAAAGTTTTCAAAGGAAGATAGTCAACATATGAAGCACTAG

mRNA sequence

ATGGAGGTGCAGAATGTAGAGCTAAAGAAGATGAGACAAGACATCGATAATATTCAGGAGCAAGTCATGGAAATTTTAAATGTCTTAAAGAATATTGTTACAGTTGGGGACATTTCTCAACCTACCAATTTAGTCGAATCTCATAATAATTCATCACCTAGGGTGGACCCACCTTTTTGTCAATCAAAAGAGGGTTTCCCTCAGCACAGTTCGTCACATGACGCTCAGCTTTCCAAGAAAGGCCAACAAGTTCCTACAAACTGCCAGGTTGAATTCACATTCAAAGATGGACAAACATCAAAGGTCAATTACTCAGATAATTTAAAAGGTAAAGAAAAAGAAAATGTTACCAATGGAAATGTTGAAGGAACTGCAGGGAAAGAATATGCTCAAAGATGGGGAGAAGCAACAGAACAGCTGTCGGTGCCTTTAATTAATAAAGAGCCGCATCCTGAAACGAATAGAAGCTCTATTAAAAGGAAAAATAAAGTCCAGGCAGTAATTTCGAAGCGCAAGGGGAAGTACCAACAAGGATTTGTGGCATACAGTCCTTCTACGGTACCCATAAACTCATTGACTCTGCAGAATGATCCACAAGCATTACCCGGTAATCATAGTAGTCGGAGGCAACTAAAGCGAGAGAGCTTCCATCCAATCCCAATATCCTACACTGAGTTGTTATCCCAATTATTTCAAGGTAATCTATTAGCTCCCGTATCCGTAGAGCCATTGCAACCACCTTATCCAAAGTGGTACGACCCGCAAGTCCACTATGATTACCATGCAGGACCTATATGTCATTCAACTGAGAACTGTACTTCACTAAAGCATAAAGTGCAAGTTCTAATGAAGGCAGGATGGCTAAAGTTTTCAAAGGAAGATAGTCAACATATGAAGCACTAG

Coding sequence (CDS)

ATGGAGGTGCAGAATGTAGAGCTAAAGAAGATGAGACAAGACATCGATAATATTCAGGAGCAAGTCATGGAAATTTTAAATGTCTTAAAGAATATTGTTACAGTTGGGGACATTTCTCAACCTACCAATTTAGTCGAATCTCATAATAATTCATCACCTAGGGTGGACCCACCTTTTTGTCAATCAAAAGAGGGTTTCCCTCAGCACAGTTCGTCACATGACGCTCAGCTTTCCAAGAAAGGCCAACAAGTTCCTACAAACTGCCAGGTTGAATTCACATTCAAAGATGGACAAACATCAAAGGTCAATTACTCAGATAATTTAAAAGGTAAAGAAAAAGAAAATGTTACCAATGGAAATGTTGAAGGAACTGCAGGGAAAGAATATGCTCAAAGATGGGGAGAAGCAACAGAACAGCTGTCGGTGCCTTTAATTAATAAAGAGCCGCATCCTGAAACGAATAGAAGCTCTATTAAAAGGAAAAATAAAGTCCAGGCAGTAATTTCGAAGCGCAAGGGGAAGTACCAACAAGGATTTGTGGCATACAGTCCTTCTACGGTACCCATAAACTCATTGACTCTGCAGAATGATCCACAAGCATTACCCGGTAATCATAGTAGTCGGAGGCAACTAAAGCGAGAGAGCTTCCATCCAATCCCAATATCCTACACTGAGTTGTTATCCCAATTATTTCAAGGTAATCTATTAGCTCCCGTATCCGTAGAGCCATTGCAACCACCTTATCCAAAGTGGTACGACCCGCAAGTCCACTATGATTACCATGCAGGACCTATATGTCATTCAACTGAGAACTGTACTTCACTAAAGCATAAAGTGCAAGTTCTAATGAAGGCAGGATGGCTAAAGTTTTCAAAGGAAGATAGTCAACATATGAAGCACTAG

Protein sequence

MEVQNVELKKMRQDIDNIQEQVMEILNVLKNIVTVGDISQPTNLVESHNNSSPRVDPPFCQSKEGFPQHSSSHDAQLSKKGQQVPTNCQVEFTFKDGQTSKVNYSDNLKGKEKENVTNGNVEGTAGKEYAQRWGEATEQLSVPLINKEPHPETNRSSIKRKNKVQAVISKRKGKYQQGFVAYSPSTVPINSLTLQNDPQALPGNHSSRRQLKRESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHAGPICHSTENCTSLKHKVQVLMKAGWLKFSKEDSQHMKH
Homology
BLAST of Moc06g31220 vs. NCBI nr
Match: XP_022153553.1 (uncharacterized protein LOC111021029 [Momordica charantia])

HSP 1 Score: 375.2 bits (962), Expect = 5.4e-100
Identity = 196/235 (83.40%), Postives = 206/235 (87.66%), Query Frame = 0

Query: 1   MEVQNVELKKMRQDIDNIQEQVMEILNVLKNIVTVGDISQPTNLVESHNNSSPRVDPPFC 60
           MEVQNVELKKMRQDIDNIQ+QVM+ILNVLKNIVTVG  SQP NLVE +NNSSPRVDPP C
Sbjct: 39  MEVQNVELKKMRQDIDNIQKQVMKILNVLKNIVTVGGTSQPNNLVEPYNNSSPRVDPPLC 98

Query: 61  QSKEGFPQHSSSHDAQLSKKGQQVPTNCQVEFTFKDGQTSKVNYSDNLKGKEKENVTNGN 120
           QSKEGFPQHSSSHDAQLSKKGQQVPTNCQVEFTFKD QT KVNYSDNLKG+EKENVT   
Sbjct: 99  QSKEGFPQHSSSHDAQLSKKGQQVPTNCQVEFTFKDEQTPKVNYSDNLKGEEKENVT--- 158

Query: 121 VEGTAGKEYAQRWGEATEQLSVPLINKEPHPETNRSSIKRKNKVQAVISKRKGKYQQGFV 180
                     QRWG+A EQL VPLINKEPHP+ N+SSIKRKNKV AV+SKRK KYQQGFV
Sbjct: 159 ----------QRWGKAIEQLPVPLINKEPHPKMNKSSIKRKNKVLAVVSKRKRKYQQGFV 218

Query: 181 AYSPSTVPINSLTLQNDPQALPGNHSSRRQLKRESFHPIPISYTELLSQLFQGNL 236
           AYSPSTVPINS+TLQ+DPQ LP NHSSRRQLKRESFH IP SYTELLSQLFQGNL
Sbjct: 219 AYSPSTVPINSMTLQDDPQTLPNNHSSRRQLKRESFHSIPTSYTELLSQLFQGNL 260

BLAST of Moc06g31220 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 127.1 bits (318), Expect = 2.6e-25
Identity = 56/92 (60.87%), Postives = 68/92 (73.91%), Query Frame = 0

Query: 203 GNHSSRRQLKRESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHA 262
           G  ++R   K+  F PIP++YTELL QLFQ N LAPV V+P+QPPYP+WYD     DYHA
Sbjct: 375 GQQNNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDANARCDYHA 434

Query: 263 GPICHSTENCTSLKHKVQVLMKAGWLKFSKED 295
           G I HSTENCT LK++VQ L+KAGW  F KE+
Sbjct: 435 GAIXHSTENCTXLKYRVQALIKAGWXNFKKEN 466

BLAST of Moc06g31220 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 126.7 bits (317), Expect = 3.3e-25
Identity = 56/89 (62.92%), Postives = 69/89 (77.53%), Query Frame = 0

Query: 206 SSRRQLKRESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHAGPI 265
           ++R   K+  F PIP++YTELL QLFQ N LAPV V+P+QPPYP+WYD     DYHAG I
Sbjct: 338 TNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDTNARCDYHAGAI 397

Query: 266 CHSTENCTSLKHKVQVLMKAGWLKFSKED 295
            HSTENCT+LK++VQ L+KAGWL F KE+
Sbjct: 398 GHSTENCTALKYRVQALIKAGWLNFKKEN 426

BLAST of Moc06g31220 vs. NCBI nr
Match: XP_022155098.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica charantia])

HSP 1 Score: 123.6 bits (309), Expect = 2.8e-24
Identity = 57/98 (58.16%), Postives = 71/98 (72.45%), Query Frame = 0

Query: 203 GNHSSRRQLKRESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHA 262
           G  ++R   K+  F PIP++YTELL QLFQ N LAPV V+P+QPPYP WYD     DYHA
Sbjct: 437 GQQNNRSTRKQSQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPGWYDANXRCDYHA 496

Query: 263 GPICHSTENCTSLKHKVQVLMKAGWLKFSKEDSQHMKH 301
           G I HSTENCT+LK++VQ L+KAG L F KE+   +K+
Sbjct: 497 GAIGHSTENCTALKYRVQALIKAGXLTFKKENXPDVKN 534

BLAST of Moc06g31220 vs. NCBI nr
Match: KAA0054657.1 (uncharacterized protein E6C27_scaffold24G004750 [Cucumis melo var. makuwa] >TYK08606.1 uncharacterized protein E5676_scaffold3734G00290 [Cucumis melo var. makuwa])

HSP 1 Score: 122.9 bits (307), Expect = 4.8e-24
Identity = 83/204 (40.69%), Postives = 105/204 (51.47%), Query Frame = 0

Query: 107 NLKGKEKENVTNGNVEGTAGKEYAQRWGEATEQLSVPLINKE-----------PHPETNR 166
           NL+  EK+NV          KEYAQRW E   Q+  PL +KE           P+ +   
Sbjct: 181 NLQRMEKKNV-------ETFKEYAQRWRELATQVQPPLTDKELMAMFINTLRAPYYDRMV 240

Query: 167 SSIKRKNKVQAVISKRKGKYQQGFVAYSPSTVPINSLTLQN---DPQALPGNHSSRRQLK 226
            S+         I +R     +    YSPS         QN    P       ++R   K
Sbjct: 241 GSVSINFSDVITIGER----IEFETNYSPS--------YQNRGKSPFGQATQRNARNSWK 300

Query: 227 RESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHAGPICHSTENC 286
           +  F PIP+SYTELL QL + + +A V  EPLQPPYPKWYDP V  +YHAG + HSTENC
Sbjct: 301 QTYFDPIPMSYTELLPQLLKSHQVAIVPQEPLQPPYPKWYDPNVKREYHAGAVGHSTENC 360

Query: 287 TSLKHKVQVLMKAGWLKFSKEDSQ 297
             LK KVQ L+KA WLKF K +++
Sbjct: 361 FPLKAKVQSLVKARWLKFKKTEAE 365

BLAST of Moc06g31220 vs. ExPASy TrEMBL
Match: A0A6J1DHS7 (uncharacterized protein LOC111021029 OS=Momordica charantia OX=3673 GN=LOC111021029 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 2.6e-100
Identity = 196/235 (83.40%), Postives = 206/235 (87.66%), Query Frame = 0

Query: 1   MEVQNVELKKMRQDIDNIQEQVMEILNVLKNIVTVGDISQPTNLVESHNNSSPRVDPPFC 60
           MEVQNVELKKMRQDIDNIQ+QVM+ILNVLKNIVTVG  SQP NLVE +NNSSPRVDPP C
Sbjct: 39  MEVQNVELKKMRQDIDNIQKQVMKILNVLKNIVTVGGTSQPNNLVEPYNNSSPRVDPPLC 98

Query: 61  QSKEGFPQHSSSHDAQLSKKGQQVPTNCQVEFTFKDGQTSKVNYSDNLKGKEKENVTNGN 120
           QSKEGFPQHSSSHDAQLSKKGQQVPTNCQVEFTFKD QT KVNYSDNLKG+EKENVT   
Sbjct: 99  QSKEGFPQHSSSHDAQLSKKGQQVPTNCQVEFTFKDEQTPKVNYSDNLKGEEKENVT--- 158

Query: 121 VEGTAGKEYAQRWGEATEQLSVPLINKEPHPETNRSSIKRKNKVQAVISKRKGKYQQGFV 180
                     QRWG+A EQL VPLINKEPHP+ N+SSIKRKNKV AV+SKRK KYQQGFV
Sbjct: 159 ----------QRWGKAIEQLPVPLINKEPHPKMNKSSIKRKNKVLAVVSKRKRKYQQGFV 218

Query: 181 AYSPSTVPINSLTLQNDPQALPGNHSSRRQLKRESFHPIPISYTELLSQLFQGNL 236
           AYSPSTVPINS+TLQ+DPQ LP NHSSRRQLKRESFH IP SYTELLSQLFQGNL
Sbjct: 219 AYSPSTVPINSMTLQDDPQTLPNNHSSRRQLKRESFHSIPTSYTELLSQLFQGNL 260

BLAST of Moc06g31220 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.2e-25
Identity = 56/92 (60.87%), Postives = 68/92 (73.91%), Query Frame = 0

Query: 203 GNHSSRRQLKRESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHA 262
           G  ++R   K+  F PIP++YTELL QLFQ N LAPV V+P+QPPYP+WYD     DYHA
Sbjct: 375 GQQNNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDANARCDYHA 434

Query: 263 GPICHSTENCTSLKHKVQVLMKAGWLKFSKED 295
           G I HSTENCT LK++VQ L+KAGW  F KE+
Sbjct: 435 GAIXHSTENCTXLKYRVQALIKAGWXNFKKEN 466

BLAST of Moc06g31220 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.6e-25
Identity = 56/89 (62.92%), Postives = 69/89 (77.53%), Query Frame = 0

Query: 206 SSRRQLKRESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHAGPI 265
           ++R   K+  F PIP++YTELL QLFQ N LAPV V+P+QPPYP+WYD     DYHAG I
Sbjct: 338 TNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDTNARCDYHAGAI 397

Query: 266 CHSTENCTSLKHKVQVLMKAGWLKFSKED 295
            HSTENCT+LK++VQ L+KAGWL F KE+
Sbjct: 398 GHSTENCTALKYRVQALIKAGWLNFKKEN 426

BLAST of Moc06g31220 vs. ExPASy TrEMBL
Match: A0A6J1DM29 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia OX=3673 GN=LOC111022231 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.4e-24
Identity = 57/98 (58.16%), Postives = 71/98 (72.45%), Query Frame = 0

Query: 203 GNHSSRRQLKRESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHA 262
           G  ++R   K+  F PIP++YTELL QLFQ N LAPV V+P+QPPYP WYD     DYHA
Sbjct: 437 GQQNNRSTRKQSQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPGWYDANXRCDYHA 496

Query: 263 GPICHSTENCTSLKHKVQVLMKAGWLKFSKEDSQHMKH 301
           G I HSTENCT+LK++VQ L+KAG L F KE+   +K+
Sbjct: 497 GAIGHSTENCTALKYRVQALIKAGXLTFKKENXPDVKN 534

BLAST of Moc06g31220 vs. ExPASy TrEMBL
Match: A0A5A7UJ84 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3734G00290 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 2.3e-24
Identity = 83/204 (40.69%), Postives = 105/204 (51.47%), Query Frame = 0

Query: 107 NLKGKEKENVTNGNVEGTAGKEYAQRWGEATEQLSVPLINKE-----------PHPETNR 166
           NL+  EK+NV          KEYAQRW E   Q+  PL +KE           P+ +   
Sbjct: 181 NLQRMEKKNV-------ETFKEYAQRWRELATQVQPPLTDKELMAMFINTLRAPYYDRMV 240

Query: 167 SSIKRKNKVQAVISKRKGKYQQGFVAYSPSTVPINSLTLQN---DPQALPGNHSSRRQLK 226
            S+         I +R     +    YSPS         QN    P       ++R   K
Sbjct: 241 GSVSINFSDVITIGER----IEFETNYSPS--------YQNRGKSPFGQATQRNARNSWK 300

Query: 227 RESFHPIPISYTELLSQLFQGNLLAPVSVEPLQPPYPKWYDPQVHYDYHAGPICHSTENC 286
           +  F PIP+SYTELL QL + + +A V  EPLQPPYPKWYDP V  +YHAG + HSTENC
Sbjct: 301 QTYFDPIPMSYTELLPQLLKSHQVAIVPQEPLQPPYPKWYDPNVKREYHAGAVGHSTENC 360

Query: 287 TSLKHKVQVLMKAGWLKFSKEDSQ 297
             LK KVQ L+KA WLKF K +++
Sbjct: 361 FPLKAKVQSLVKARWLKFKKTEAE 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153553.15.4e-10083.40uncharacterized protein LOC111021029 [Momordica charantia][more]
XP_022147189.12.6e-2560.87LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia][more]
XP_022158986.13.3e-2562.92LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia][more]
XP_022155098.12.8e-2458.16LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica ch... [more]
KAA0054657.14.8e-2440.69uncharacterized protein E6C27_scaffold24G004750 [Cucumis melo var. makuwa] >TYK0... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DHS72.6e-10083.40uncharacterized protein LOC111021029 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A6J1D0991.2e-2560.87Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1[more]
A0A6J1E2J71.6e-2562.92Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1[more]
A0A6J1DM291.4e-2458.16LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia... [more]
A0A5A7UJ842.3e-2440.69Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..83

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g31220.1Moc06g31220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071897 DNA biosynthetic process
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0034061 DNA polymerase activity
molecular_function GO:0003676 nucleic acid binding