Moc06g30820 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g30820
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUlp1-like peptidase
Locationchr6: 23212474 .. 23215626 (+)
RNA-Seq ExpressionMoc06g30820
SyntenyMoc06g30820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATATAGCCGCACGATTCGAGGATGACTTTGATGTATTCTCCACCAATATGTTCACAGCTTTGAAGTACGAGCCGATGACAACTCTTTCTTCATTTATGCATGACAATGTTGAGGATGTAATTGTGCATGAGATAGAAGCATCACATGGTGATCACGAGGGCGTCCAACCTGAACTAGAATTGGTTTTCATTTTGTTCCGTCTTAATTATGTTCTAATGAAGTTTGCATGTTTCCTATTTGAAGCAACATGCATGTGACTTGTCGTTGAAGGAAGACGTGAATAAAGAGGATGGGTAGGAGGAGAGTAGGGTAACAGAAGGCAGAAGGCGTGAATGTTTCTCGTGGCAAGGATGCGCGTGGCAAGGATACAACGATTGAACTTATTATCATCGATTCACAGTACTTTGAGTCATTCACAAACGAGGTATTTGATGGGGTGAATCCCGAGCAGAAGCGGTAGAACGAGTTCATGAATTTTCTATTTGAAATTTGGAACACAATAAAGCTAAAATAGAGGGAAGAATGAGTTTACCTTTATAGAACCTATTCTTCACAATTACCAATCGACACCACCATAGGGTCTTCCTTACTAAGTCTCCTGCAAGAAATCCGATTTGTGGGAACCAAGTGAGGGAAAAGAAAGTTGTTGTGTGAGAGAGAACATTTTCTCTGTTTTCTTTTTCAAGTGGAAGAAGAGAACCCGTGCAAAACCATTCTGCACATTAGGAAGGGGCAGGTGACGTGGGGAGTTACCCCACGTCATAACAACCGTCCACTTCCCTACTAAAATGCAAAAGAGTTAATTAATAATAATAATAATAATAAATTAATTTAATTAAATCTATATATTAATTAAATGAATAATTAACAATTAATTATTTAATTTTTAATTAAATATAACATGTTTAATTAAAATTATATATTTGAATCATATTCAAATCTTTCTCTCTGGTTATAACCTATAGTTTTAATATGAATCCAATTCATATTAATATTTCTCTCATAATACATGATATTAATTTTGAATCTTTATCCAAAATAATGTATATTTATATAATATATTAATATACATTATAACATTCTCTTATAAAATTTAAACATTTCAAATTATTTCATAATTCTTGATCCTCGTTTCACCTGTTGTGAGCTAGCAAGGGGACCAAATGGACCTACAGATCAGAAGCTCCAACGATATGAGATTAATTGATTAAACTCATTAACCAAATTAACCAACTTTCGTTAACTGCAGATCGCTCCACTAAAGATCCGTAACTGCACTCTTCTCACTGCATAATATTTTGTGTCCACGAAAAATTAACCATTGATGGCAAGTCAATCTTTCACGAGTATTCGTAACTTCAGTTGGGTCAAATTATCGTTTTACTCCTGAGTTACATCTTGCACCTTAAATACCATTACTCCTCTAATGAACAACATGTTTATGGTCCGACCATAAAACAGAGGGAAGTTTTTCTTGGGCCAGTGACAGGGTGGGACCATTTGTTCAAATCTTAGAGTCAGCATTTAAGGAGTGAATTCCATCTTGCGTAGTTATATTCCCAGCTCCCGCTTAGTCTCGTCCTAAAATGGCAGGATTCTTAAGTCGACAGTCCGAGTCACTCTCACCCATACAAATCAAAGGACGATCCCTTATAGGTAGGAGTCCATAACTCGTTCAGGATTAAGATCGAGTTACCTGGTCATCATATGAAATATTAGTCTAGTTAGTTTACAATGTTGTAATAAAAGATTAATTATTTCACAATCCGACCTTATACAAACTCATTGTATATGATATCTCTACTTGCATGTCTCTACATGAATGACTTGGATAGAGTCGTTTGTAACTATTACAAAACAGGCTGTATCATAATGTCACCAGGATAAGGTACCCAACCTTATTCATGTATTATAAATAATTTAGGTTATTACTGAAACATTATCCACTTGTATATCAAGTATATATTGTATATTTATTATATACTGCATATCAAACGTATATACTATATATTAAATGTATACCGTATATCAATATATACTGTTCCAATTACAATAAATAACCTTAGATCTTAGTTTATTGGAATACGAGTAATGCAAAAAAAAAAAAAAACTCTATATTGAATAAATAAATAATTTGTTCATTAATTACAAATACACGAGTTAAAGGTACCAAACCTAATAATATTTACTCTTGACAGTCGACCTATCAATCCAATACTTCTTCTTTTAACATATTTTATGCAGGACCAATCCGTGGACAATTACTTAGACTCTGATCTGGAAGAAATAAGAGAAGTTAAATCCCAGATTGATGAGCATACATCCGACACGTCGCAGGTGGACTCAGATATGGTACGTGTGCTCTCTCAGTTGATTGATCGCTCTCAACCTCGATGAGGGTCGCAGAAGAGGAAATTGTCATGGAAACTTCGAGGCTCATTCCTAGTTATGGTGGATGGGAAGAGAAAGAAGGTAACTCGGTACAATTCACTAGTCCAAATCCCCCCCCCCCTTTCCGGACACGTCAATAAGTTTCATGCATGGATGGACAACCCTAGGACTAACCATTCGACTCGTAAGTCATGCAATCGTGATCGAGGAAAGACATGGTTTCGTGACCTCATCCCGACAAGTGGTTGACGAGTGAGGTACAAGGTCGAAACCTTAAATGTGATTACGTTGTTGATTACAATTTTCTAACAATAGTAACCTATGTAGGTGATTGATTCGTTGATTATGTTCACGAGGAACAAGATCGAGCAATGTTCAGACTTGTGTTGTCGAAAGTTTACCACCGATGACATAGTTCTTGCAATACGATATCTATATAATATACGTTGCCGTACGTAATAATTCATGTTCTAACATGTCTTCGTGTCCAACCACATAACTTATTTCGACGAATCGATGTAATATACCAACACTTGATAGCCTCGAATGTTGTTCCATTGAGAGTTGCAACGGATTACGATTGGGCCGGCAAATATAAAATTGTTCTTAGCTACATAGACGACACTCACACAGACTACCAGAAATGGTGGCTTGAATTGGACGTTGTGTACTTATTGTATAACATCGATGGAAACCATTGGATCATGGTTTGCATCGATCTGGAGAAGGGGGAGATTGCCCGAGGTTTTGTTCAGTAG

mRNA sequence

ATGTATATAGCCGCACGATTCGAGGATGACTTTGATGTATTCTCCACCAATATGTTCACAGCTTTGAAGTACGAGCCGATGACAACTCTTTCTTCATTTATGCATGACAATGTTGAGGATGTAATTGTGCATGAGATAGAAGCATCACATGGTGATCACGAGGGCGTCCAACCTGAACTAGAATTGGTTTTCATTTTGTTCCGTCTTAATTATGACCAATCCGTGGACAATTACTTAGACTCTGATCTGGAAGAAATAAGAGAAGTTAAATCCCAGATTGATGAGCATACATCCGACACGTCGCAGGTGGACTCAGATATGGTGATTGATTCGTTGATTATGTTCACGAGGAACAAGATCGAGCAATGTTCAGACTTGTGTTGTCGAAAGTTTACCACCGATGACATAGTTCTTGCAATACGATATCTATATAATATACGTTGCCCCTCGAATGTTGTTCCATTGAGAGTTGCAACGGATTACGATTGGGCCGGCAAATATAAAATTGTTCTTAGCTACATAGACGACACTCACACAGACTACCAGAAATGGTGGCTTGAATTGGACGTTGTGTACTTATTGTATAACATCGATGGAAACCATTGGATCATGGTTTGCATCGATCTGGAGAAGGGGGAGATTGCCCGAGGTTTTGTTCAGTAG

Coding sequence (CDS)

ATGTATATAGCCGCACGATTCGAGGATGACTTTGATGTATTCTCCACCAATATGTTCACAGCTTTGAAGTACGAGCCGATGACAACTCTTTCTTCATTTATGCATGACAATGTTGAGGATGTAATTGTGCATGAGATAGAAGCATCACATGGTGATCACGAGGGCGTCCAACCTGAACTAGAATTGGTTTTCATTTTGTTCCGTCTTAATTATGACCAATCCGTGGACAATTACTTAGACTCTGATCTGGAAGAAATAAGAGAAGTTAAATCCCAGATTGATGAGCATACATCCGACACGTCGCAGGTGGACTCAGATATGGTGATTGATTCGTTGATTATGTTCACGAGGAACAAGATCGAGCAATGTTCAGACTTGTGTTGTCGAAAGTTTACCACCGATGACATAGTTCTTGCAATACGATATCTATATAATATACGTTGCCCCTCGAATGTTGTTCCATTGAGAGTTGCAACGGATTACGATTGGGCCGGCAAATATAAAATTGTTCTTAGCTACATAGACGACACTCACACAGACTACCAGAAATGGTGGCTTGAATTGGACGTTGTGTACTTATTGTATAACATCGATGGAAACCATTGGATCATGGTTTGCATCGATCTGGAGAAGGGGGAGATTGCCCGAGGTTTTGTTCAGTAG

Protein sequence

MYIAARFEDDFDVFSTNMFTALKYEPMTTLSSFMHDNVEDVIVHEIEASHGDHEGVQPELELVFILFRLNYDQSVDNYLDSDLEEIREVKSQIDEHTSDTSQVDSDMVIDSLIMFTRNKIEQCSDLCCRKFTTDDIVLAIRYLYNIRCPSNVVPLRVATDYDWAGKYKIVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEIARGFVQ
Homology
BLAST of Moc06g30820 vs. NCBI nr
Match: XP_022148308.1 (uncharacterized protein LOC111016993 [Momordica charantia])

HSP 1 Score: 119.4 bits (298), Expect = 3.9e-23
Identity = 54/112 (48.21%), Postives = 74/112 (66.07%), Query Frame = 0

Query: 108 VIDSLIMFTRNKIEQCSDLCCRKFTTDDIVLAIRY-----LYNIRCPSNVVPLRVATDYD 167
           V+D L M  R K+EQ  DLC RKFTT D+VLA  +     +Y      + +P +VA +YD
Sbjct: 137 VVDELFMLLRKKLEQRPDLCSRKFTTGDLVLANYFRRKDDJYACMQSDSSLPSKVAAEYD 196

Query: 168 WAGKYKIVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
           W G+ + ++ Y D THTDY   W+E+D +Y+ +NI G HW+MVCIDLE+GEI
Sbjct: 197 WDGRDESIMGYTDGTHTDYPLRWMEVDAIYIPFNIRGKHWVMVCIDLEEGEI 248

BLAST of Moc06g30820 vs. NCBI nr
Match: XP_022132578.1 (uncharacterized protein LOC111005406 [Momordica charantia])

HSP 1 Score: 115.5 bits (288), Expect = 5.6e-22
Identity = 54/106 (50.94%), Postives = 70/106 (66.04%), Query Frame = 0

Query: 114 MFTRNKIEQCSDLCCRKFTTDDIVLAIRY-----LYNIRCPSNVVPLRVATDYDWAGKYK 173
           M  R K+EQ  DLC RKFTT D+VLA  +     LY      + +P +VA +YDW G+ K
Sbjct: 1   MLVRKKLEQRPDLCSRKFTTGDLVLANYFRRKDGLYACMQSDSSLPSKVAAEYDWDGRDK 60

Query: 174 IVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
            ++ Y D TH DY   W+E+DV+YL +NI G HW+MVCIDLE+GEI
Sbjct: 61  SIMGYTDGTHMDYPLRWMEVDVIYLPFNIRGKHWVMVCIDLEEGEI 106

BLAST of Moc06g30820 vs. NCBI nr
Match: XP_022154364.1 (uncharacterized protein LOC111021646 [Momordica charantia])

HSP 1 Score: 102.4 bits (254), Expect = 4.9e-18
Identity = 48/106 (45.28%), Postives = 70/106 (66.04%), Query Frame = 0

Query: 114 MFTRNKIEQCSDLCCRKFTTDDIVL-----AIRYLYNIRCPSNVVPLRVATDYDWAGKYK 173
           MF  NK++   +LC RKFTT D+++     +   +Y +    NV+  RVA+DYDW G+  
Sbjct: 1   MFVCNKLKLRPNLCRRKFTTGDVLISNFLRSTDGVYVMMQSPNVIASRVASDYDWEGRAW 60

Query: 174 IVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
            +LSYID TH+D    W+++D VYL YNI G HWI++CID ++GE+
Sbjct: 61  SMLSYIDGTHSDNDTRWMDVDAVYLPYNIGGVHWIVICIDFDEGEL 106

BLAST of Moc06g30820 vs. NCBI nr
Match: XP_022158807.1 (uncharacterized protein LOC111025273 [Momordica charantia])

HSP 1 Score: 69.7 bits (169), Expect = 3.5e-08
Identity = 38/111 (34.23%), Postives = 57/111 (51.35%), Query Frame = 0

Query: 106 DMVIDSLIMFTRNKIEQCSDLCCRKFTTDDIVLA--IRYLYNIRCPSNVVPLRVATDYDW 165
           D  IDSL+M T  K+E+C  L   +F   D++L+  +R             L     YDW
Sbjct: 35  DEYIDSLMMLTARKVEKCKHLLRVQFAIGDVLLSNLLRRTDGPYAAMKPGVLPSKCTYDW 94

Query: 166 AGKYKIVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
             + + +  Y+    +DY   W E D+VY   NI GNHW+M+ IDL +G++
Sbjct: 95  R-QERTIFRYVLGRQSDYDTLWSEADIVYTRMNIGGNHWVMIGIDLVEGDL 144

BLAST of Moc06g30820 vs. NCBI nr
Match: XP_022159362.1 (uncharacterized protein LOC111025779 [Momordica charantia])

HSP 1 Score: 66.6 bits (161), Expect = 3.0e-07
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0

Query: 174 IDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
           +D THTDYQ  WL+LDVVYL YNI GNHWIMV IDL+  EI
Sbjct: 1   MDGTHTDYQTRWLDLDVVYLPYNIGGNHWIMVHIDLQDDEI 41

BLAST of Moc06g30820 vs. ExPASy TrEMBL
Match: A0A6J1D3R7 (uncharacterized protein LOC111016993 OS=Momordica charantia OX=3673 GN=LOC111016993 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.4e-23
Identity = 55/112 (49.11%), Postives = 74/112 (66.07%), Query Frame = 0

Query: 108 VIDSLIMFTRNKIEQCSDLCCRKFTTDDIVLAIRY-----LYNIRCPSNVVPLRVATDYD 167
           V+D L M  R K+EQ  DLC RKFTT D+VLA  +     LY      + +P +VA +YD
Sbjct: 137 VVDELFMLLRKKLEQRPDLCSRKFTTGDLVLANYFRRKDDLYACMQSDSSLPSKVAAEYD 196

Query: 168 WAGKYKIVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
           W G+ + ++ Y D THTDY   W+E+D +Y+ +NI G HW+MVCIDLE+GEI
Sbjct: 197 WDGRDESIMGYTDGTHTDYPLRWMEVDAIYIPFNIRGKHWVMVCIDLEEGEI 248

BLAST of Moc06g30820 vs. ExPASy TrEMBL
Match: A0A6J1BWN0 (uncharacterized protein LOC111005406 OS=Momordica charantia OX=3673 GN=LOC111005406 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 2.7e-22
Identity = 54/106 (50.94%), Postives = 70/106 (66.04%), Query Frame = 0

Query: 114 MFTRNKIEQCSDLCCRKFTTDDIVLAIRY-----LYNIRCPSNVVPLRVATDYDWAGKYK 173
           M  R K+EQ  DLC RKFTT D+VLA  +     LY      + +P +VA +YDW G+ K
Sbjct: 1   MLVRKKLEQRPDLCSRKFTTGDLVLANYFRRKDGLYACMQSDSSLPSKVAAEYDWDGRDK 60

Query: 174 IVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
            ++ Y D TH DY   W+E+DV+YL +NI G HW+MVCIDLE+GEI
Sbjct: 61  SIMGYTDGTHMDYPLRWMEVDVIYLPFNIRGKHWVMVCIDLEEGEI 106

BLAST of Moc06g30820 vs. ExPASy TrEMBL
Match: A0A6J1DLV0 (uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021646 PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.4e-18
Identity = 48/106 (45.28%), Postives = 70/106 (66.04%), Query Frame = 0

Query: 114 MFTRNKIEQCSDLCCRKFTTDDIVL-----AIRYLYNIRCPSNVVPLRVATDYDWAGKYK 173
           MF  NK++   +LC RKFTT D+++     +   +Y +    NV+  RVA+DYDW G+  
Sbjct: 1   MFVCNKLKLRPNLCRRKFTTGDVLISNFLRSTDGVYVMMQSPNVIASRVASDYDWEGRAW 60

Query: 174 IVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
            +LSYID TH+D    W+++D VYL YNI G HWI++CID ++GE+
Sbjct: 61  SMLSYIDGTHSDNDTRWMDVDAVYLPYNIGGVHWIVICIDFDEGEL 106

BLAST of Moc06g30820 vs. ExPASy TrEMBL
Match: A0A6J1DY60 (uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025273 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.7e-08
Identity = 38/111 (34.23%), Postives = 57/111 (51.35%), Query Frame = 0

Query: 106 DMVIDSLIMFTRNKIEQCSDLCCRKFTTDDIVLA--IRYLYNIRCPSNVVPLRVATDYDW 165
           D  IDSL+M T  K+E+C  L   +F   D++L+  +R             L     YDW
Sbjct: 35  DEYIDSLMMLTARKVEKCKHLLRVQFAIGDVLLSNLLRRTDGPYAAMKPGVLPSKCTYDW 94

Query: 166 AGKYKIVLSYIDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
             + + +  Y+    +DY   W E D+VY   NI GNHW+M+ IDL +G++
Sbjct: 95  R-QERTIFRYVLGRQSDYDTLWSEADIVYTRMNIGGNHWVMIGIDLVEGDL 144

BLAST of Moc06g30820 vs. ExPASy TrEMBL
Match: A0A6J1DZM8 (uncharacterized protein LOC111025779 OS=Momordica charantia OX=3673 GN=LOC111025779 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.5e-07
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0

Query: 174 IDDTHTDYQKWWLELDVVYLLYNIDGNHWIMVCIDLEKGEI 215
           +D THTDYQ  WL+LDVVYL YNI GNHWIMV IDL+  EI
Sbjct: 1   MDGTHTDYQTRWLDLDVVYLPYNIGGNHWIMVHIDLQDDEI 41

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148308.13.9e-2348.21uncharacterized protein LOC111016993 [Momordica charantia][more]
XP_022132578.15.6e-2250.94uncharacterized protein LOC111005406 [Momordica charantia][more]
XP_022154364.14.9e-1845.28uncharacterized protein LOC111021646 [Momordica charantia][more]
XP_022158807.13.5e-0834.23uncharacterized protein LOC111025273 [Momordica charantia][more]
XP_022159362.13.0e-0773.17uncharacterized protein LOC111025779 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1D3R71.4e-2349.11uncharacterized protein LOC111016993 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A6J1BWN02.7e-2250.94uncharacterized protein LOC111005406 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1DLV02.4e-1845.28uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A6J1DY601.7e-0834.23uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DZM81.5e-0773.17uncharacterized protein LOC111025779 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 79..99
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 181..214
e-value: 1.3E-4
score: 21.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g30820.1Moc06g30820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity