Moc06g29730 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc06g29730 vs. NCBI nr
Match: XP_022150863.1 (uncharacterized protein LOC111018910 [Momordica charantia])

HSP 1 Score: 570.1 bits (1468), Expect = 2.6e-158
Identity = 337/602 (55.98%), Postives = 373/602 (61.96%), Query Frame = 0

Query: 1   MNPPNPNIRQPIPPNVRIKEIVDGVPVAANSEVVVPPLNAVLVVDGIDREIRAYAAPAFY 60
           MNPPNPN+ QPIPPNVRI+EIVDGVPVA N EV+VP LN VL+   IDREIRAYAAP FY
Sbjct: 1   MNPPNPNLHQPIPPNVRIEEIVDGVPVATNHEVIVPSLNVVLLAKAIDREIRAYAAPTFY 60

Query: 61  NFNPVITEPEIAAPKFELKPVMFQMLQTMG-QFHDHPTEDPHSHLKTWLESLPSESITSW 120
           NFNPVITE EI APKFELK       + +  +   H   D     +TWL SLPSESITSW
Sbjct: 61  NFNPVITEREITAPKFELKSDEGYNKEVLRLKLFTHSLRD---EARTWLMSLPSESITSW 120

Query: 121 EDLAEKFLMKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKCLHHGIPRCIHI 180
           +DLAE FLMKYF  SK AKYRSDINNFQQFTGESV+ESWE FKRL+QKCLHH IPRCIHI
Sbjct: 121 DDLAEIFLMKYFPPSKNAKYRSDINNFQQFTGESVTESWEHFKRLMQKCLHHRIPRCIHI 180

Query: 181 EAYYNGLDDATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPRAIQSRGGKGLN 240
           E YYNGLDDATRLV   S N ALLAKPYAEAFNILERISSN HS SD RAIQ RG K LN
Sbjct: 181 EMYYNGLDDATRLVNVVSPNEALLAKPYAEAFNILERISSNKHSRSDTRAIQGRGTKRLN 240

Query: 241 ESESYSALNSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFYEGDHHYNNCPN 300
           ES+SYS  NSKIEN+IDLV RSMTQQSTVGA TGKAN  H QG S SF+ G HHYNNCP 
Sbjct: 241 ESKSYSTPNSKIENVIDLVRRSMTQQSTVGAPTGKANASHSQGFSYSFWHGYHHYNNCPG 300

Query: 301 NPESVYCLENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGNYPP 360
           NPESVYCL N  NS+NN YS TYNPG RNHPN                            
Sbjct: 301 NPESVYCLGNTYNSRNNSYSNTYNPGSRNHPN---------------------------- 360

Query: 361 GFTSQGLVAAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQVGQLATDLKSY 420
                                                     +R   + V  +   +   
Sbjct: 361 ----------------------------------------XEIREEIMLVHPMLQHISRK 420

Query: 421 GALPSNTEVLKRDGKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKESGESQLAKNNEPA 480
           G +    +VL+                         +K +         E Q    N   
Sbjct: 421 GVI---YQVLR-------------------------IKIV---------EQQRENQNSSN 480

Query: 481 ETTVPTSPVQIMEEPRKVKDITKAEGTPTEHADVSAEYRPAPPYPKRLQKKEQNIQFKKF 540
           E   P + +  +    +++   K +   TEH D  AEY+ APPYPKR QKKE+N+QF KF
Sbjct: 481 EEVNPVNRLASIAGSTQIRVSKKRK--QTEHEDALAEYKLAPPYPKRFQKKEENVQFMKF 492

Query: 541 LDVLKQLHVNIPLVEALEQMPNYVRFLKEILTKKRKLGEYETVTMTKACSNILMSKIPAK 600
           LDVLKQLHVNIPLVEALE+M NYVRFLK+ILTKK KLGEYETV MTKACS IL SKIP K
Sbjct: 541 LDVLKQLHVNIPLVEALEKMQNYVRFLKKILTKKIKLGEYETVVMTKACSTILTSKIPTK 492

Query: 601 MK 602
           MK
Sbjct: 601 MK 492

BLAST of Moc06g29730 vs. NCBI nr
Match: XP_022158611.1 (uncharacterized protein LOC111025065 [Momordica charantia])

HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-145
Identity = 298/534 (55.81%), Postives = 332/534 (62.17%), Query Frame = 0

Query: 82  MFQMLQTMGQFHDHPTEDPHSHLK---------------------------------TWL 141
           MFQMLQT+ QFH H TEDPH HLK                                 TWL
Sbjct: 1   MFQMLQTVDQFHGHATEDPHQHLKFFMGVCNSFKNEGLSNEVLRLKLFPYSLRDEARTWL 60

Query: 142 ESLPSESITSWEDLAEKFLMKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKC 201
           ESLP ESITSW+DLAEKFLMKYF  SK AKYRS+INNFQQF GESVSESWE FKRLLQ C
Sbjct: 61  ESLPLESITSWDDLAEKFLMKYFPPSKNAKYRSEINNFQQFAGESVSESWECFKRLLQSC 120

Query: 202 LHHGIPRCIHIEAYYNGLDDATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPR 261
            HHGIPRCI IE YY  L+DATRL                                 DPR
Sbjct: 121 PHHGIPRCIQIETYYKDLNDATRL--------------------------------PDPR 180

Query: 262 AIQSRGGKGLNESESYSALNSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFY 321
           A+Q +  KGL ESESY+ LNS IENL  LVMRSM QQS+VGA TG ANV  IQGISCSF 
Sbjct: 181 AVQGKSSKGLVESESYTTLNSNIENLTVLVMRSMMQQSSVGALTGTANVNQIQGISCSFC 240

Query: 322 EGDHHYNNCPNNPESVYCLENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNG 381
           EGDHHYNNCP NPESVY L NPQN++NN YS TYNPGWRNHPNFSWSG+QGG+N GTS+ 
Sbjct: 241 EGDHHYNNCPGNPESVYYLGNPQNNRNNLYSNTYNPGWRNHPNFSWSGDQGGHNAGTSSA 300

Query: 382 SAYQPKGNYPPGFTSQGLVAAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQ 441
            A+Q K +YPPGF +QG + A+  +EG+IASLEKLMKQYM NNDAT++SQA SLRNL+LQ
Sbjct: 301 PAFQHKVSYPPGFVNQGQMVARRQSEGSIASLEKLMKQYMANNDATVQSQATSLRNLKLQ 360

Query: 442 VGQLATDLKSYGALPSNTEVLKRDGKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKESG 501
           VGQLATDLKS                                                  
Sbjct: 361 VGQLATDLKS-------------------------------------------------- 420

Query: 502 ESQLAKNNEPAETTVPTSPVQIMEEPRKVKDITKAEGTPTEHADVSAEYRPAPPYPKRLQ 561
                             P+++ E+ ++            EH +  AEY PAPPYPKRLQ
Sbjct: 421 -----------------KPIRVPEKRKQ-----------AEHENAPAEYSPAPPYPKRLQ 424

Query: 562 KKEQNIQFKKFLDVLKQLHVNIPLVEALEQMPNYVRFLKEILTKKRKLGEYETV 583
           KKE+N+QF KFLDVLKQLHVNIPLVEALEQMPNYVRFLKEIL KKR LGEY+T+
Sbjct: 481 KKERNVQFNKFLDVLKQLHVNIPLVEALEQMPNYVRFLKEILIKKRTLGEYDTI 424

BLAST of Moc06g29730 vs. NCBI nr
Match: XP_017233063.1 (PREDICTED: uncharacterized protein LOC108207110 [Daucus carota subsp. sativus])

HSP 1 Score: 464.5 bits (1194), Expect = 1.6e-126
Identity = 278/616 (45.13%), Postives = 374/616 (60.71%), Query Frame = 0

Query: 30  NSEVVVPPLNAVLVVDGIDREIRAYAAPAFYNFNPVITEPEIAAPKFELKPVMFQMLQTM 89
           N ++ + P  A  +VD  DR IR YAAP F   N  I  P I A +FELKPVMFQMLQT+
Sbjct: 42  NGDIPIVPRGA-FIVDDKDRAIRQYAAPRFEELNSGIIRPNIQATQFELKPVMFQMLQTI 101

Query: 90  GQFHDHPTEDPHSHL---------------------------------KTWLESLPSESI 149
           GQF   PTEDPH HL                                 +TWL SLP+ S+
Sbjct: 102 GQFSGMPTEDPHLHLRLFMEISDSFKFQGVPEDALRLKLFPYSVRDRARTWLNSLPAGSV 161

Query: 150 TSWEDLAEKFLMKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKCLHHGIPRC 209
           T+W DL EKFL KYF  +  AK R++IN+FQQ   ES+ ++WERFK LL+KC HHGI  C
Sbjct: 162 TTWNDLTEKFLSKYFPPNMNAKLRNEINSFQQQDDESLYDAWERFKELLRKCPHHGILHC 221

Query: 210 IHIEAYYNGLDDATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPRAIQSRGGK 269
           I +E +YNGL+  T++V+DASANGALL+K Y +A+ ILE I++ N+  S  RA   +   
Sbjct: 222 IQMETFYNGLNAQTKMVVDASANGALLSKSYNQAYEILETIATKNYQWSSSRAQTGKKVA 281

Query: 270 GLNESESYSALNSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFYEGDHHYNN 329
           G+ + +S +++ +++ ++  ++       +     +  + +   + +SC F    H Y++
Sbjct: 282 GIYDVDSITSMKAQLASMEHMLKNLSMGNNQSKEQSLSSQINQTKNVSCVFCGEAHTYDS 341

Query: 330 CPNNPESVYCLENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGN 389
           CP+NPESV+ + N   +K  PYS TYN  WR HPNFSWS NQG N+ GTS G+    K N
Sbjct: 342 CPSNPESVFYMGN--QNKAGPYSNTYNQSWRQHPNFSWS-NQGANS-GTSTGNV---KSN 401

Query: 390 YPPGFTSQGLVAAQEPAEGTIASLEKLMKQYMTNNDAT-------LKSQAASLRNLELQV 449
           YPPGF+ Q   A Q        SLE ++K+Y+  N+A+       ++SQAASLRNLE QV
Sbjct: 402 YPPGFSQQ---APQS------NSLENMLKEYIIKNEASRSQTEALVQSQAASLRNLENQV 461

Query: 450 GQLATDLKS--YGALPSNTEVLKRDGKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKES 509
           GQLA +L++  +G LPS+TE  K  G E CKA+TL+SGK L  T   V  A    + + S
Sbjct: 462 GQLANELRNRPHGTLPSDTEKPKGVGNEHCKAMTLKSGKVLGNT---VTDAKYDDSVEPS 521

Query: 510 GESQLAKNNEPAETTVPTSPVQIMEEPRKVKDITKAEGTPTEHADVSAEYRPAPPYPKRL 569
           G  ++  + E     V  SP      P+  K                +  +P PP+P+R 
Sbjct: 522 GNEEIPDDKESENDKV--SP------PKSSK---------------KSHIQPQPPFPQRF 581

Query: 570 QKKEQNIQFKKFLDVLKQLHVNIPLVEALEQMPNYVRFLKEILTKKRKLGEYETVTMTKA 604
           QK++QN+QFKKFLDVLKQLH+NIPLVEALEQMPNYV+F+K+ILTKKR+LGE+ETV +TK 
Sbjct: 582 QKQKQNVQFKKFLDVLKQLHINIPLVEALEQMPNYVKFMKDILTKKRRLGEFETVALTKE 614

BLAST of Moc06g29730 vs. NCBI nr
Match: XP_030505184.1 (uncharacterized protein LOC115720166 [Cannabis sativa])

HSP 1 Score: 456.4 bits (1173), Expect = 4.2e-124
Identity = 272/604 (45.03%), Postives = 371/604 (61.42%), Query Frame = 0

Query: 38  LNAVLVVDGIDREIRAYAAPAFYNFNPVITEPEIAAPKFELKPVMFQMLQTMGQFHDHPT 97
           ++ +++VD   R IR YAAP F   NP I  PEI AP+FELKPVMFQMLQT+GQF + PT
Sbjct: 15  VSPIILVDDRARAIREYAAPMFNELNPGIVRPEIQAPQFELKPVMFQMLQTVGQFSEMPT 74

Query: 98  EDPHSHL---------------------------------KTWLESLPSESITSWEDLAE 157
           EDPH HL                                 ++WL +L  +S+T+W D AE
Sbjct: 75  EDPHLHLRSFLEMSDSFKIQGVSEEVRRLKLFPFSLRDRARSWLNTLSPDSVTNWNDFAE 134

Query: 158 KFLMKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKCLHHGIPRCIHIEAYYN 217
           KFL KYF  ++ AK+RS+I +F Q   ES S++WERFK LL+KC HHGIP CI +E +YN
Sbjct: 135 KFLRKYFPPTRNAKFRSEIMSFHQLEDESASDAWERFKELLRKCPHHGIPHCIQMETFYN 194

Query: 218 GLDDATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPRAIQSRGGKGLNESESY 277
           GL+  +++V+DASANGA+L+K Y EAF ILE I+SNN+  S+ RA  SR   G+ E ++ 
Sbjct: 195 GLNATSQMVLDASANGAILSKSYNEAFEILETIASNNYQWSNTRAPGSRKVAGVLEVDAI 254

Query: 278 SALNSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQG--ISCSFYEGDHHYNNCPNNPE 337
           +AL +++ +     M ++ +  ++G S        IQ   +SC F    H +  CP+NPE
Sbjct: 255 TALTTQMAS-----MTNVLKNLSIGNSKNIQPAAAIQSDDVSCVFCREGHAFEKCPSNPE 314

Query: 338 SVYCLENPQNSKNN-PYSITYNPGWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGNYPPGF 397
           SV  + N   ++NN  +S +YN  W+NHPN SW       +         Q +  YPPGF
Sbjct: 315 SVCYMGNQNFNRNNGAFSNSYNQAWKNHPNLSWGSRSKLKHFD-------QGRQAYPPGF 374

Query: 398 TSQGLVAAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQVGQLATDLKS--Y 457
            SQ L   Q       +SLE LM+ YM  NDA ++SQAA LRNLELQ+G LA +LK+   
Sbjct: 375 -SQQLRHPQHAQNSQPSSLESLMRDYMAKNDAVIQSQAAFLRNLELQLGHLANELKARPQ 434

Query: 458 GALPSNTEVLKRDGKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKESGESQLAKNNEPA 517
           G+LPS+TE  +RDGKEQCK++ LRSGK L  +    +K   +PT  +  E +L+K     
Sbjct: 435 GSLPSDTENPRRDGKEQCKSIHLRSGKHLKNSE-EEIKGSGEPTSIQIDE-KLSKKT--- 494

Query: 518 ETTVPTSPVQIMEEPRKVKDITKAEGTPTEHADVSAEYRPAPPYPKRLQKKEQNIQFKKF 577
                    Q + + R+V   T  +    + A V +  +P  P+P+R QK++Q+ QFKKF
Sbjct: 495 --------AQEIADTRRVDTATGQQSNSQQSAPVCSSSKPPLPFPQRFQKQQQDGQFKKF 554

Query: 578 LDVLKQLHVNIPLVEALEQMPNYVRFLKEILTKKRKLGEYETVTMTKACSNILMSKIPAK 604
           LDVLKQLH+NIPLVEALEQMPNYV+FLK+ILTKKR+LGE+E+  +T+    +L +KIP K
Sbjct: 555 LDVLKQLHINIPLVEALEQMPNYVKFLKDILTKKRRLGEFES-RLTEGQKAMLKNKIPPK 591

BLAST of Moc06g29730 vs. NCBI nr
Match: XP_022156835.1 (uncharacterized protein LOC111023669 [Momordica charantia])

HSP 1 Score: 434.1 bits (1115), Expect = 2.3e-117
Identity = 220/284 (77.46%), Postives = 238/284 (83.80%), Query Frame = 0

Query: 128 MKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKCLHHGIPRCIHIEAYYNGLD 187
           MKYF   K AKYRS+I NFQQ   ESV+ESWERFK+LLQKC HHGIPRCI IE YY GLD
Sbjct: 1   MKYFPPRKNAKYRSEIKNFQQIVRESVNESWERFKQLLQKCPHHGIPRCIQIEKYYKGLD 60

Query: 188 DATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPRAIQSRGGKGLNESESYSAL 247
           DATRLVIDAS NGALL KPYAEAFNILERISSNNHS SDPRAIQ RGGKGLNESESY AL
Sbjct: 61  DATRLVIDASTNGALLVKPYAEAFNILERISSNNHSWSDPRAIQGRGGKGLNESESYYAL 120

Query: 248 NSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFYEGDHHYNNCPNNPESVYCL 307
           NSK+ENL +LVMRSMTQQ+TVGAS GKANV HIQGISCSF EG+HHYNN P+NPESVY L
Sbjct: 121 NSKVENLTNLVMRSMTQQNTVGASDGKANVSHIQGISCSFCEGEHHYNNYPDNPESVYYL 180

Query: 308 ENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGNYPPGFTSQGLV 367
            N QN+  N YS TYNPGWRNHPNFSWSGNQGGNN GTSN  AYQ K +YPP F++QG V
Sbjct: 181 GNAQNNGKNSYSNTYNPGWRNHPNFSWSGNQGGNNAGTSNAPAYQQKESYPPDFSNQGQV 240

Query: 368 AAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQVGQ 412
             Q  +EG+ ASLE LMK+ M  ND T++SQAASLRNLE+QVGQ
Sbjct: 241 TVQMVSEGSFASLENLMKKNMEKNDITVQSQAASLRNLEMQVGQ 284

BLAST of Moc06g29730 vs. ExPASy TrEMBL
Match: A0A6J1DAK9 (uncharacterized protein LOC111018910 OS=Momordica charantia OX=3673 GN=LOC111018910 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 1.3e-158
Identity = 337/602 (55.98%), Postives = 373/602 (61.96%), Query Frame = 0

Query: 1   MNPPNPNIRQPIPPNVRIKEIVDGVPVAANSEVVVPPLNAVLVVDGIDREIRAYAAPAFY 60
           MNPPNPN+ QPIPPNVRI+EIVDGVPVA N EV+VP LN VL+   IDREIRAYAAP FY
Sbjct: 1   MNPPNPNLHQPIPPNVRIEEIVDGVPVATNHEVIVPSLNVVLLAKAIDREIRAYAAPTFY 60

Query: 61  NFNPVITEPEIAAPKFELKPVMFQMLQTMG-QFHDHPTEDPHSHLKTWLESLPSESITSW 120
           NFNPVITE EI APKFELK       + +  +   H   D     +TWL SLPSESITSW
Sbjct: 61  NFNPVITEREITAPKFELKSDEGYNKEVLRLKLFTHSLRD---EARTWLMSLPSESITSW 120

Query: 121 EDLAEKFLMKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKCLHHGIPRCIHI 180
           +DLAE FLMKYF  SK AKYRSDINNFQQFTGESV+ESWE FKRL+QKCLHH IPRCIHI
Sbjct: 121 DDLAEIFLMKYFPPSKNAKYRSDINNFQQFTGESVTESWEHFKRLMQKCLHHRIPRCIHI 180

Query: 181 EAYYNGLDDATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPRAIQSRGGKGLN 240
           E YYNGLDDATRLV   S N ALLAKPYAEAFNILERISSN HS SD RAIQ RG K LN
Sbjct: 181 EMYYNGLDDATRLVNVVSPNEALLAKPYAEAFNILERISSNKHSRSDTRAIQGRGTKRLN 240

Query: 241 ESESYSALNSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFYEGDHHYNNCPN 300
           ES+SYS  NSKIEN+IDLV RSMTQQSTVGA TGKAN  H QG S SF+ G HHYNNCP 
Sbjct: 241 ESKSYSTPNSKIENVIDLVRRSMTQQSTVGAPTGKANASHSQGFSYSFWHGYHHYNNCPG 300

Query: 301 NPESVYCLENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGNYPP 360
           NPESVYCL N  NS+NN YS TYNPG RNHPN                            
Sbjct: 301 NPESVYCLGNTYNSRNNSYSNTYNPGSRNHPN---------------------------- 360

Query: 361 GFTSQGLVAAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQVGQLATDLKSY 420
                                                     +R   + V  +   +   
Sbjct: 361 ----------------------------------------XEIREEIMLVHPMLQHISRK 420

Query: 421 GALPSNTEVLKRDGKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKESGESQLAKNNEPA 480
           G +    +VL+                         +K +         E Q    N   
Sbjct: 421 GVI---YQVLR-------------------------IKIV---------EQQRENQNSSN 480

Query: 481 ETTVPTSPVQIMEEPRKVKDITKAEGTPTEHADVSAEYRPAPPYPKRLQKKEQNIQFKKF 540
           E   P + +  +    +++   K +   TEH D  AEY+ APPYPKR QKKE+N+QF KF
Sbjct: 481 EEVNPVNRLASIAGSTQIRVSKKRK--QTEHEDALAEYKLAPPYPKRFQKKEENVQFMKF 492

Query: 541 LDVLKQLHVNIPLVEALEQMPNYVRFLKEILTKKRKLGEYETVTMTKACSNILMSKIPAK 600
           LDVLKQLHVNIPLVEALE+M NYVRFLK+ILTKK KLGEYETV MTKACS IL SKIP K
Sbjct: 541 LDVLKQLHVNIPLVEALEKMQNYVRFLKKILTKKIKLGEYETVVMTKACSTILTSKIPTK 492

Query: 601 MK 602
           MK
Sbjct: 601 MK 492

BLAST of Moc06g29730 vs. ExPASy TrEMBL
Match: A0A6J1E1F3 (uncharacterized protein LOC111025065 OS=Momordica charantia OX=3673 GN=LOC111025065 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 5.5e-146
Identity = 298/534 (55.81%), Postives = 332/534 (62.17%), Query Frame = 0

Query: 82  MFQMLQTMGQFHDHPTEDPHSHLK---------------------------------TWL 141
           MFQMLQT+ QFH H TEDPH HLK                                 TWL
Sbjct: 1   MFQMLQTVDQFHGHATEDPHQHLKFFMGVCNSFKNEGLSNEVLRLKLFPYSLRDEARTWL 60

Query: 142 ESLPSESITSWEDLAEKFLMKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKC 201
           ESLP ESITSW+DLAEKFLMKYF  SK AKYRS+INNFQQF GESVSESWE FKRLLQ C
Sbjct: 61  ESLPLESITSWDDLAEKFLMKYFPPSKNAKYRSEINNFQQFAGESVSESWECFKRLLQSC 120

Query: 202 LHHGIPRCIHIEAYYNGLDDATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPR 261
            HHGIPRCI IE YY  L+DATRL                                 DPR
Sbjct: 121 PHHGIPRCIQIETYYKDLNDATRL--------------------------------PDPR 180

Query: 262 AIQSRGGKGLNESESYSALNSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFY 321
           A+Q +  KGL ESESY+ LNS IENL  LVMRSM QQS+VGA TG ANV  IQGISCSF 
Sbjct: 181 AVQGKSSKGLVESESYTTLNSNIENLTVLVMRSMMQQSSVGALTGTANVNQIQGISCSFC 240

Query: 322 EGDHHYNNCPNNPESVYCLENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNG 381
           EGDHHYNNCP NPESVY L NPQN++NN YS TYNPGWRNHPNFSWSG+QGG+N GTS+ 
Sbjct: 241 EGDHHYNNCPGNPESVYYLGNPQNNRNNLYSNTYNPGWRNHPNFSWSGDQGGHNAGTSSA 300

Query: 382 SAYQPKGNYPPGFTSQGLVAAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQ 441
            A+Q K +YPPGF +QG + A+  +EG+IASLEKLMKQYM NNDAT++SQA SLRNL+LQ
Sbjct: 301 PAFQHKVSYPPGFVNQGQMVARRQSEGSIASLEKLMKQYMANNDATVQSQATSLRNLKLQ 360

Query: 442 VGQLATDLKSYGALPSNTEVLKRDGKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKESG 501
           VGQLATDLKS                                                  
Sbjct: 361 VGQLATDLKS-------------------------------------------------- 420

Query: 502 ESQLAKNNEPAETTVPTSPVQIMEEPRKVKDITKAEGTPTEHADVSAEYRPAPPYPKRLQ 561
                             P+++ E+ ++            EH +  AEY PAPPYPKRLQ
Sbjct: 421 -----------------KPIRVPEKRKQ-----------AEHENAPAEYSPAPPYPKRLQ 424

Query: 562 KKEQNIQFKKFLDVLKQLHVNIPLVEALEQMPNYVRFLKEILTKKRKLGEYETV 583
           KKE+N+QF KFLDVLKQLHVNIPLVEALEQMPNYVRFLKEIL KKR LGEY+T+
Sbjct: 481 KKERNVQFNKFLDVLKQLHVNIPLVEALEQMPNYVRFLKEILIKKRTLGEYDTI 424

BLAST of Moc06g29730 vs. ExPASy TrEMBL
Match: A0A6J1DRG1 (uncharacterized protein LOC111023669 OS=Momordica charantia OX=3673 GN=LOC111023669 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.1e-117
Identity = 220/284 (77.46%), Postives = 238/284 (83.80%), Query Frame = 0

Query: 128 MKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKCLHHGIPRCIHIEAYYNGLD 187
           MKYF   K AKYRS+I NFQQ   ESV+ESWERFK+LLQKC HHGIPRCI IE YY GLD
Sbjct: 1   MKYFPPRKNAKYRSEIKNFQQIVRESVNESWERFKQLLQKCPHHGIPRCIQIEKYYKGLD 60

Query: 188 DATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPRAIQSRGGKGLNESESYSAL 247
           DATRLVIDAS NGALL KPYAEAFNILERISSNNHS SDPRAIQ RGGKGLNESESY AL
Sbjct: 61  DATRLVIDASTNGALLVKPYAEAFNILERISSNNHSWSDPRAIQGRGGKGLNESESYYAL 120

Query: 248 NSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFYEGDHHYNNCPNNPESVYCL 307
           NSK+ENL +LVMRSMTQQ+TVGAS GKANV HIQGISCSF EG+HHYNN P+NPESVY L
Sbjct: 121 NSKVENLTNLVMRSMTQQNTVGASDGKANVSHIQGISCSFCEGEHHYNNYPDNPESVYYL 180

Query: 308 ENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGNYPPGFTSQGLV 367
            N QN+  N YS TYNPGWRNHPNFSWSGNQGGNN GTSN  AYQ K +YPP F++QG V
Sbjct: 181 GNAQNNGKNSYSNTYNPGWRNHPNFSWSGNQGGNNAGTSNAPAYQQKESYPPDFSNQGQV 240

Query: 368 AAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQVGQ 412
             Q  +EG+ ASLE LMK+ M  ND T++SQAASLRNLE+QVGQ
Sbjct: 241 TVQMVSEGSFASLENLMKKNMEKNDITVQSQAASLRNLEMQVGQ 284

BLAST of Moc06g29730 vs. ExPASy TrEMBL
Match: A0A6J0ZX64 (LOW QUALITY PROTEIN: uncharacterized protein LOC110412945 OS=Herrania umbratica OX=108875 GN=LOC110412945 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 2.1e-108
Identity = 247/600 (41.17%), Postives = 346/600 (57.67%), Query Frame = 0

Query: 39  NAVLVVDGIDREIRAYAAPAFYNFNPVITEPEIAAPKFELKPVMFQMLQTMGQFHDHPTE 98
           NA+ +V   +R +R Y  P     +  I  P I A  FE+KP   QM+Q+  QF   P++
Sbjct: 47  NAINLVPEANRALRDYVVPLVQGLHQSIRRPSINANNFEIKPAYIQMIQSSVQFSGLPSD 106

Query: 99  DPHSHL---------------------------------KTWLESLPSESITSWEDLAEK 158
           DP+SHL                                 K+WL SLP+ SIT+WEDLA+K
Sbjct: 107 DPNSHLVNFLEICDTFKYNGVTDDAIRLRLFPFSLRDKAKSWLNSLPNGSITTWEDLAQK 166

Query: 159 FLMKYFLSSKKAKYRSDINNFQQFTGESVSESWERFKRLLQKCLHHGIPRCIHIEAYYNG 218
           FL K+F  +K AK R+DI +F QF GES+ E+WERFK LL++C HHGIP  + ++ +YNG
Sbjct: 167 FLAKFFPPAKTAKMRNDITSFIQFDGESLYEAWERFKELLRRCPHHGIPDWLQVQTFYNG 226

Query: 219 LDDATRLVIDASANGALLAKPYAEAFNILERISSNNHSCSDPRAIQSRGGKGLNESESYS 278
           L  + + +IDA+A GAL++K   +A+N+LE ++SNN+     R+  SR   G  E ++  
Sbjct: 227 LVGSIKTIIDAAAGGALMSKNAVDAYNLLEEMASNNYQWPSERS-GSRKAVGAYEIDALG 286

Query: 279 ALNSKIENLIDLVMRSMTQQSTVGASTGKANVRHIQGISCSFYEGDHHYNNCPNNPESVY 338
            L +++  L         +  T+G    + ++     + C      H Y+ CP N ESV 
Sbjct: 287 TLTTQVAAL-------SKKLDTLGVHAVQNSL-----VVCEMCGDSHSYDQCPYNSESVQ 346

Query: 339 CLENPQNSKNNPYSITYNPGWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGNYPPGFTSQG 398
            + N    +NNPYS TYNPGWRNHPNFSWS     NN G SN     PK   PPGF  Q 
Sbjct: 347 FVGNFNRQQNNPYSNTYNPGWRNHPNFSWS-----NNAGPSN-----PKPIMPPGFQQQA 406

Query: 399 LVAAQEPAEGTIASLEKLMKQYMTNNDATLKSQAASLRNLELQVGQLATDL--KSYGALP 458
                E      + LE+L+ QY++  DA ++SQ ASLRNLE QVGQLA  +  +  G+LP
Sbjct: 407 RPQIPEKK----SQLEELLLQYISKTDAIIQSQGASLRNLETQVGQLANSINNRPQGSLP 466

Query: 459 SNTEVLKRDGKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKESGESQLAKNNEPAETTV 518
           S+T++  + GKEQC+A+TLRSGK +   +   +++  +   KE     + +N        
Sbjct: 467 SDTQINPK-GKEQCQAITLRSGKEIEGVNQKAVESEIEHVDKEG----MCENE------- 526

Query: 519 PTSPVQIMEEPRKVKDITKAEGTPTEHADVSAEYRPAPPYPKRLQKKEQNIQFKKFLDVL 578
               ++I +     KD  KAE   T     S    P PP+P+RLQK++   QF+KFL+V 
Sbjct: 527 ----IEIQQ-----KDDDKAENQGT-----SQVIHPPPPFPQRLQKQKLEKQFQKFLNVF 586

Query: 579 KQLHVNIPLVEALEQMPNYVRFLKEILTKKRKLGEYETVTMTKACSNILMSKIPAKMKDP 604
           K+LH+NIP  EALEQMP+YV+FLK+IL+KKRKLGE+ETV +T+ CS IL +K+P K+KDP
Sbjct: 587 KKLHINIPFAEALEQMPSYVKFLKDILSKKRKLGEFETVFLTEECSAILQNKLPPKLKDP 593

BLAST of Moc06g29730 vs. ExPASy TrEMBL
Match: A0A6J1EQ90 (uncharacterized protein LOC111436411 OS=Cucurbita moschata OX=3662 GN=LOC111436411 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 4.0e-104
Identity = 243/656 (37.04%), Postives = 350/656 (53.35%), Query Frame = 0

Query: 10  QPIPPNVRIKEIVDGVPVAANSEVVVPPLNAVLVVDGIDREIRAYAAPAFYNFNPVITEP 69
           Q I    ++    +   + AN E +    N + + D  +R IRAYA PA    NP I  P
Sbjct: 35  QQIELGAQLNREFENPAMMANQERIT--ANPIHLADDRERAIRAYAHPAVEELNPCIIRP 94

Query: 70  EIAAPKFELKPVMFQMLQTMGQFHDHPTEDPHSHL------------------------- 129
           EI    FELKPVMFQMLQT+GQFH  P EDPH HL                         
Sbjct: 95  EIQGTTFELKPVMFQMLQTIGQFHGLPLEDPHLHLKSFLGVSDSFRFHSDSFRFQGVDKD 154

Query: 130 ---------------KTWLESLPSESITSWEDLAEKFLMKYFLSSKKAKYRSDINNFQQF 189
                          K+WL +L   +I SW  LAE FL+KYF  ++ A+++++I  FQQF
Sbjct: 155 MIRLSLFPYLLRDGAKSWLNTLAPGTIDSWNSLAENFLIKYFPPTRNARFKNEIVTFQQF 214

Query: 190 TGESVSESWERFKRLLQKCLHHGIPRCIHIEAYYNGLDDATRLVIDASANGALLAKPYAE 249
             E++SE+ ERFK +L+KC HHG+P CI +E +YNGL+  T+ V+DASANGA+L+K Y E
Sbjct: 215 EDETLSEACERFKEMLRKCPHHGLPHCIQMETFYNGLNIVTKQVVDASANGAILSKTYNE 274

Query: 250 AFNILERISSNNHSCSDPRAIQSRGGKGLNESESYSALNSKIENLIDLVMR-SMTQQSTV 309
           A+ ILERI+SNN   +D R+   R  +G+ E ++ S++N+++ ++ +++   ++ Q S +
Sbjct: 275 AYEILERIASNNCQWADVRSNPGRKTRGVLEVDALSSINAQLASVTNILQNLALGQDSMI 334

Query: 310 GAST-GKANVRHIQGISCSFYEGDHHYNNCPNNPESVYCLENPQ---NSKNNPYSITYNP 369
            A     A +      SC +   +H ++ CP+NP S++ + N     N KNNP+S TYNP
Sbjct: 335 KAPVHTAAAINQTAAESCVYCGEEHTFDQCPSNPASIFYVGNQASQGNLKNNPFSNTYNP 394

Query: 370 GWRNHPNFSWSGNQGGNNVGTSNGSAYQPKGNYPPGFTSQGLVA-----AQEPAEGTI-- 429
           GWRNHPNFSW G    N           PK NYP GF  Q  +A          +GT   
Sbjct: 395 GWRNHPNFSWKGQSLYN-------QQMPPKANYPSGFRLQNQLAYSSQQVNTQGKGTTQA 454

Query: 430 -----ASLEKLMKQYMTNNDATLKSQAASLRNLELQVGQLATDLKSYGALPSNTEVLKRD 489
                 S+E L+K+YM  NDA ++SQ ASLRNLE+Q+G                      
Sbjct: 455 QYTSETSIESLIKEYMAKNDAVIQSQQASLRNLEVQIG---------------------- 514

Query: 490 GKEQCKALTLRSGKALPPTHLNVLKAMSKPTPKESGESQLAKNNEPAETTVPTSPVQIME 549
           G++                              E G+S    + E A+T        + +
Sbjct: 515 GEKNA----------------------------EQGDSH---SQETADTQQRNEEAAVQK 574

Query: 550 EPRKVKDITKAEGTPTEHADVSAE-----YRPAPPYPKRLQKKEQNIQFKKFLDVLKQLH 604
           E    KD  + E  P      S+E     Y P+PP+P+R+++K++   F+KF+D+LK++H
Sbjct: 575 E--HSKDYAEVEEQPKMQTTASSEQESRTYTPSPPFPQRIKRKKEEAHFEKFMDILKEIH 626