Moc06g29440 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc06g29440 vs. NCBI nr
Match: XP_022151683.1 (uncharacterized protein LOC111019598 [Momordica charantia])

HSP 1 Score: 166.0 bits (419), Expect = 5.5e-37
Identity = 122/371 (32.88%), Postives = 165/371 (44.47%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+  + +LWTA+QELFGVQSRA+ DYL+QVFQQT K SL+M++YL++MKSH  NL  AG
Sbjct: 127 MGFSTSRELWTAVQELFGVQSRAEVDYLKQVFQQTCKGSLQMIEYLKLMKSHADNLALAG 186

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTLTTPKLTIVGRPNILIIIKGVVE 120
           S  S   L+SQVL GLDEEY P+V  +QG   ++W  +    LT   R      +K  + 
Sbjct: 187 SSVSVRDLVSQVLTGLDEEYNPIVVAVQGKVNLSWSEMHAELLTYEKRLEYQNSLKSGIP 246

Query: 121 AEVVAKGLIRIKERLEIPMVHMHKNGGNN------------------------PSLAPHA 180
                   +   +           NG N+                        P    H 
Sbjct: 247 INQTQTPSVNYVDGRSFQTNQRTNNGNNSHGSNTHRGGGYQRGSFGQRNRGRGPQPTQHK 306

Query: 181 FLTTQNINP----------FVANSETVLDPNWYVDNGASNHVIADYHNIIHPSEY----- 240
             T  N  P           V   ETV+DP+WY D+GA++HV A+ +N+    +Y     
Sbjct: 307 NFTPSNSGPNVFAAHHTSTTVTTPETVIDPSWYADSGATSHVTANPNNVEQKVDYSGTEN 366

Query: 241 ---------------------GGHSFEQGDA-------------------EGSLKDELYQ 285
                                 G S +  D                    +G+LKD LY+
Sbjct: 367 VIVANGNKLSISHIGSTNIHASGGSLKLKDVLRVPDIAKNLDKASGRTLLKGTLKDNLYR 426

BLAST of Moc06g29440 vs. NCBI nr
Match: XP_022148963.1 (uncharacterized protein LOC111017501 [Momordica charantia])

HSP 1 Score: 155.6 bits (392), Expect = 7.4e-34
Identity = 77/95 (81.05%), Postives = 81/95 (85.26%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MGYENA DLW AIQELFGVQS+A+EDYLRQVFQQT K SLKM D+LRVMKSH  NLGQAG
Sbjct: 62  MGYENACDLWAAIQELFGVQSQAEEDYLRQVFQQTRKGSLKMTDFLRVMKSHADNLGQAG 121

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITW 96
           SP  T SLISQVLLGLDEEY PVVA IQG  GI+W
Sbjct: 122 SPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISW 156

BLAST of Moc06g29440 vs. NCBI nr
Match: KAA0026100.1 (uncharacterized protein E6C27_scaffold19G00360 [Cucumis melo var. makuwa])

HSP 1 Score: 155.6 bits (392), Expect = 7.4e-34
Identity = 102/287 (35.54%), Postives = 141/287 (49.13%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+ N  DLW A Q+ FGVQSRA+ED+LRQ+ Q T K + KM +YL VMK++V NLGQ G
Sbjct: 132 MGFTNVEDLWDATQDFFGVQSRAEEDFLRQMLQTTRKGNTKMEEYLLVMKTNVDNLGQVG 191

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTL---------------------- 120
           SP    +LISQVLLGLDE Y  V+ +IQG   I+WL +                      
Sbjct: 192 SPVPRRALISQVLLGLDEVYNLVIVVIQGKPDISWLDMQSKLLIFEKILKHQNTQKKKKK 251

Query: 121 -----TTPKLTIVGR--------------------------------PNILIIIKGVVEA 180
                 +P L +  R                                P   +  K    A
Sbjct: 252 KGNITQSPALNMAQRFALNGQRNHSNKKFYGYNRQHFSGQRGNLNNGPTCQLCGKYGHSA 311

Query: 181 EVVAKGLIRIKERLEIPMVHMHKNGGNNPSLAPH--AFLTTQNINPFVANSETVLDPNWY 227
            V      R  +    P+V       +N S++P+   F++TQN  PF A  +TV+DPNWY
Sbjct: 312 LVCYN---RFNKEFSSPLVQDRNEHSSNGSVSPNPAVFVSTQNATPF-ATPDTVVDPNWY 371

BLAST of Moc06g29440 vs. NCBI nr
Match: KAA0057475.1 (uncharacterized protein E6C27_scaffold280G003560 [Cucumis melo var. makuwa] >TYK30171.1 uncharacterized protein E5676_scaffold216G001590 [Cucumis melo var. makuwa])

HSP 1 Score: 153.3 bits (386), Expect = 3.7e-33
Identity = 91/209 (43.54%), Postives = 127/209 (60.77%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+  A DLW AIQ LFG++SRA+E +LR  FQ T + + KM DYLR+MK +  NLGQAG
Sbjct: 96  MGFNTAKDLWEAIQNLFGIKSRAEEYFLRHTFQTTREGNYKMEDYLRIMKINADNLGQAG 155

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTLTTPKLTIVGRPNILIIIKGVVE 120
           SP     LISQVLLGLDE Y PV A+IQG   I+WL + +  L      N++ I+   +E
Sbjct: 156 SPVPHRYLISQVLLGLDEVYNPVTAVIQGKPDISWLDMQSELLIF---ENLVEIVLIKME 215

Query: 121 AEVVAKGLIRIKERLEIPMVHMHKNGGNNPS-----LAPHAFLTTQNINPFVANSETVLD 180
           +E +      ++E          +N G NP+     +   AF+TTQ  +  +A  ETV+D
Sbjct: 216 SETILMAADVVEE----------ENRGFNPNQNGKQIPDDAFITTQK-SSSLATPETVVD 275

Query: 181 PNWYVDNGASNHVIADYHNIIHPSEYGGH 205
            N YVD+GA+NHV +D+ N+ +  +Y G+
Sbjct: 276 TNRYVDSGATNHVTSDHSNLWNIDDYSGN 290

BLAST of Moc06g29440 vs. NCBI nr
Match: TYK05754.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 140.2 bits (352), Expect = 3.2e-29
Identity = 96/269 (35.69%), Postives = 133/269 (49.44%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+ NA DLW A Q+LFGVQSRA+ED+LRQ+FQ T K      DYLR+MK++   LGQAG
Sbjct: 50  MGFTNAKDLWEATQDLFGVQSRAEEDFLRQMFQTTRKVRASYEDYLRIMKTNSDKLGQAG 109

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTLTTPKLTIVGR-------PNILI 120
           SP    + ISQ LLGLDE Y PV+A+IQG   I+W+ + +  LT   R        N   
Sbjct: 110 SPVPKRAFISQALLGLDEVYNPVIAVIQGKPEISWIDMQSELLTFEKRLEHQDTQKNTEN 169

Query: 121 IIKGVVE------------------------------------------------AEVVA 180
           II+ VV                                                  +V  
Sbjct: 170 IIQNVVNIAQNRNSSDFRKYSNHQFHGNNRNNSQGQRGGFNIGRGRGKGRGNKPTCQVCE 229

Query: 181 K----GLI---RIKERLEIPMVHMHKNGGNNPSLAPHAFLTT----QNINPFVANSETVL 204
           K     L+   R  +    P+V     G  + + + H+ LT     Q++N F A ++TV+
Sbjct: 230 KYGHSALVCYNRFNKEFLSPLV--QDRGAQSSNFSKHSNLTVLVTGQSVNQF-ATADTVI 289

BLAST of Moc06g29440 vs. ExPASy TrEMBL
Match: A0A6J1DCW4 (uncharacterized protein LOC111019598 OS=Momordica charantia OX=3673 GN=LOC111019598 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 2.7e-37
Identity = 122/371 (32.88%), Postives = 165/371 (44.47%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+  + +LWTA+QELFGVQSRA+ DYL+QVFQQT K SL+M++YL++MKSH  NL  AG
Sbjct: 127 MGFSTSRELWTAVQELFGVQSRAEVDYLKQVFQQTCKGSLQMIEYLKLMKSHADNLALAG 186

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTLTTPKLTIVGRPNILIIIKGVVE 120
           S  S   L+SQVL GLDEEY P+V  +QG   ++W  +    LT   R      +K  + 
Sbjct: 187 SSVSVRDLVSQVLTGLDEEYNPIVVAVQGKVNLSWSEMHAELLTYEKRLEYQNSLKSGIP 246

Query: 121 AEVVAKGLIRIKERLEIPMVHMHKNGGNN------------------------PSLAPHA 180
                   +   +           NG N+                        P    H 
Sbjct: 247 INQTQTPSVNYVDGRSFQTNQRTNNGNNSHGSNTHRGGGYQRGSFGQRNRGRGPQPTQHK 306

Query: 181 FLTTQNINP----------FVANSETVLDPNWYVDNGASNHVIADYHNIIHPSEY----- 240
             T  N  P           V   ETV+DP+WY D+GA++HV A+ +N+    +Y     
Sbjct: 307 NFTPSNSGPNVFAAHHTSTTVTTPETVIDPSWYADSGATSHVTANPNNVEQKVDYSGTEN 366

Query: 241 ---------------------GGHSFEQGDA-------------------EGSLKDELYQ 285
                                 G S +  D                    +G+LKD LY+
Sbjct: 367 VIVANGNKLSISHIGSTNIHASGGSLKLKDVLRVPDIAKNLDKASGRTLLKGTLKDNLYR 426

BLAST of Moc06g29440 vs. ExPASy TrEMBL
Match: A0A5A7SIT7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00360 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 3.6e-34
Identity = 102/287 (35.54%), Postives = 141/287 (49.13%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+ N  DLW A Q+ FGVQSRA+ED+LRQ+ Q T K + KM +YL VMK++V NLGQ G
Sbjct: 132 MGFTNVEDLWDATQDFFGVQSRAEEDFLRQMLQTTRKGNTKMEEYLLVMKTNVDNLGQVG 191

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTL---------------------- 120
           SP    +LISQVLLGLDE Y  V+ +IQG   I+WL +                      
Sbjct: 192 SPVPRRALISQVLLGLDEVYNLVIVVIQGKPDISWLDMQSKLLIFEKILKHQNTQKKKKK 251

Query: 121 -----TTPKLTIVGR--------------------------------PNILIIIKGVVEA 180
                 +P L +  R                                P   +  K    A
Sbjct: 252 KGNITQSPALNMAQRFALNGQRNHSNKKFYGYNRQHFSGQRGNLNNGPTCQLCGKYGHSA 311

Query: 181 EVVAKGLIRIKERLEIPMVHMHKNGGNNPSLAPH--AFLTTQNINPFVANSETVLDPNWY 227
            V      R  +    P+V       +N S++P+   F++TQN  PF A  +TV+DPNWY
Sbjct: 312 LVCYN---RFNKEFSSPLVQDRNEHSSNGSVSPNPAVFVSTQNATPF-ATPDTVVDPNWY 371

BLAST of Moc06g29440 vs. ExPASy TrEMBL
Match: A0A6J1D5J0 (uncharacterized protein LOC111017501 OS=Momordica charantia OX=3673 GN=LOC111017501 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 3.6e-34
Identity = 77/95 (81.05%), Postives = 81/95 (85.26%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MGYENA DLW AIQELFGVQS+A+EDYLRQVFQQT K SLKM D+LRVMKSH  NLGQAG
Sbjct: 62  MGYENACDLWAAIQELFGVQSQAEEDYLRQVFQQTRKGSLKMTDFLRVMKSHADNLGQAG 121

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITW 96
           SP  T SLISQVLLGLDEEY PVVA IQG  GI+W
Sbjct: 122 SPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISW 156

BLAST of Moc06g29440 vs. ExPASy TrEMBL
Match: A0A5D3E3L7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001590 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 1.8e-33
Identity = 91/209 (43.54%), Postives = 127/209 (60.77%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+  A DLW AIQ LFG++SRA+E +LR  FQ T + + KM DYLR+MK +  NLGQAG
Sbjct: 96  MGFNTAKDLWEAIQNLFGIKSRAEEYFLRHTFQTTREGNYKMEDYLRIMKINADNLGQAG 155

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTLTTPKLTIVGRPNILIIIKGVVE 120
           SP     LISQVLLGLDE Y PV A+IQG   I+WL + +  L      N++ I+   +E
Sbjct: 156 SPVPHRYLISQVLLGLDEVYNPVTAVIQGKPDISWLDMQSELLIF---ENLVEIVLIKME 215

Query: 121 AEVVAKGLIRIKERLEIPMVHMHKNGGNNPS-----LAPHAFLTTQNINPFVANSETVLD 180
           +E +      ++E          +N G NP+     +   AF+TTQ  +  +A  ETV+D
Sbjct: 216 SETILMAADVVEE----------ENRGFNPNQNGKQIPDDAFITTQK-SSSLATPETVVD 275

Query: 181 PNWYVDNGASNHVIADYHNIIHPSEYGGH 205
            N YVD+GA+NHV +D+ N+ +  +Y G+
Sbjct: 276 TNRYVDSGATNHVTSDHSNLWNIDDYSGN 290

BLAST of Moc06g29440 vs. ExPASy TrEMBL
Match: A0A5D3C373 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G002430 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.6e-29
Identity = 96/269 (35.69%), Postives = 133/269 (49.44%), Query Frame = 0

Query: 1   MGYENASDLWTAIQELFGVQSRAKEDYLRQVFQQTHKSSLKMVDYLRVMKSHVVNLGQAG 60
           MG+ NA DLW A Q+LFGVQSRA+ED+LRQ+FQ T K      DYLR+MK++   LGQAG
Sbjct: 50  MGFTNAKDLWEATQDLFGVQSRAEEDFLRQMFQTTRKVRASYEDYLRIMKTNSDKLGQAG 109

Query: 61  SPASTHSLISQVLLGLDEEYKPVVAMIQGSTGITWLTLTTPKLTIVGR-------PNILI 120
           SP    + ISQ LLGLDE Y PV+A+IQG   I+W+ + +  LT   R        N   
Sbjct: 110 SPVPKRAFISQALLGLDEVYNPVIAVIQGKPEISWIDMQSELLTFEKRLEHQDTQKNTEN 169

Query: 121 IIKGVVE------------------------------------------------AEVVA 180
           II+ VV                                                  +V  
Sbjct: 170 IIQNVVNIAQNRNSSDFRKYSNHQFHGNNRNNSQGQRGGFNIGRGRGKGRGNKPTCQVCE 229

Query: 181 K----GLI---RIKERLEIPMVHMHKNGGNNPSLAPHAFLTT----QNINPFVANSETVL 204
           K     L+   R  +    P+V     G  + + + H+ LT     Q++N F A ++TV+
Sbjct: 230 KYGHSALVCYNRFNKEFLSPLV--QDRGAQSSNFSKHSNLTVLVTGQSVNQF-ATADTVI 289

BLAST of Moc06g29440 vs. TAIR 10
Match: ATMG00300.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.9e-04
Identity = 22/64 (34.38%), Postives = 31/64 (48.44%), Query Frame = 0

Query: 270 WHRRLGHPSSKVLDLLVKICLILVKVNDIFKFSEAFQFGKSHALPLANSNSHATTPCDLV 329
           WH RL H S + ++LLVK   +        KF E   +GK+H +  +        P D V
Sbjct: 72  WHSRLAHMSQRGMELLVKKGFLDSSKVSSLKFCEDCIYGKTHRVNFSTGQHTTKNPLDYV 131

Query: 330 HTDL 334
           H+DL
Sbjct: 132 HSDL 135