Moc06g19770 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g19770
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionReverse transcriptase
Locationchr6: 15511686 .. 15513165 (-)
RNA-Seq ExpressionMoc06g19770
SyntenyMoc06g19770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGTAGTGAAACAGTTGAACAAGAAACATGTCGAACGACTTGTGCAAATTGAGGAACAACTATTGTTCTTAAGAGAAGTCCCTGATCATGTTCGGTTCTTAGAAAATAGGCTAGAAGAGTTGGCTAGCAAAGCCGATGGAATCGATGCGACAAATGCTCGAATTGACGCATTGCCAGTGAAAGAGTTGTTGGTTAAGGTTGAGACCCTAGAAAACAAAGCTGGGAAAATGACTAGTTTCGAGCGAGAGGACAACTCGACAAGCTTTGTTGCTCATGTAGAGGAGCGAGTTGAAGAATTAGATTGCTCCCAGAAAGCGATCATGCAGATGGTCAGCGACCTTTTCGACGACTTTCGAGCTGCTATAGAAGTCGTGCTGGCAGAAGTCGCCGAGATAAACATGAAGGTGAACCTCACCATGAGAGTGGTGGGAAACCAAGGCCCAGCATGAGGCAATGTCCAGTTTAACAAGGTGAAGATTCCAGAGCCTAAGTCCTTTGGCGGAACTTGCGATGCCAAGGCCCTCGAGAACTTCATCTTTAACGTCGAACAATACTTTCGAGCTATGAGTACAACGACAGAAGAAGCCAAGATCACTTTGGCAACCATGCATCTAGCTGAAGATGCAAAGCTTTGGTGGAGGTCCAAGTATATGGACATCCAAGAGGGTCGATGCACTATAAACACATGGGATGAGTTGAAGAAAGCTCTAAGGGCACAGTTCTTTTCGGAGAATGTTGAGATTCTAGCGAGACGTAAACTGCGAGAGTTGAGACACACTAGGTACATTAGAGACTACGTGAAACAGTTCGCGGCACTGATGTTGGACATACGAGACATGTCAGAGAAAGACAAAGTCTTCTATTAGTGGAAGGCTTGAAACCATGGGCCAAGTTCAAGTTGTATGATCAAGAGGTTCAAGACCTCTGCTCTGCTTATGCTGCGGCTGAAAGCCTGTTTGATCTTATAATGATCGGTCACAAGACAACAGAAGGAACCAACCCTCTTCGAGTAGTGGGAACAAACCCACCAGAACAAACTCACCCAAAAGTGGGGGAGGAGGCAGGAATGAAGGAGCAGACCGCAGACCCTATCAGAGGGGTGGAACCAACTGGCGAGGGCAAAACCAACAGAACAACTTCAATCGAGGACCCTTTGTTTGCTTTCTGTGTAAAGGGGCTTATCGCATAAGCGACTGCCTGCATCGAGCTGCCCTGAAGAAACTCCAAGCTACAATAGCCAATGGTCCTGAGGTCGAGAATGAAGTTGTCGAGACAGAGATACCGACCGAAGAGCCTACTGATAACCTCTGCATTGGGGCACTGAAATTTTTGTCGGCTCTCCAGAAGAAAGTAGGGGAAGCAAAAAAACAGCTAAAGAGAGGGCTCATGTATGTGGACGTGTTGGTTCACGGTAAAGCTGCTAAGAGTACTATGGTGGATTCTGGAGCCACACACAACTTCATGGCTGAAATCTAG

mRNA sequence

ATGTCGGTAGTGAAACAGTTGAACAAGAAACATGTCGAACGACTTGTGCAAATTGAGGAACAACTATTGTTCTTAAGAGAAGTCCCTGATCATGTTCGGTTCTTAGAAAATAGGCTAGAAGAGTTGGCTAGCAAAGCCGATGGAATCGATGCGACAAATGCTCGAATTGACGCATTGCCAGTGAAAGAGTTGTTGGTTAAGGTTGAGACCCTAGAAAACAAAGCTGGGAAAATGACTAGTTTCGAGCGAGAGGACAACTCGACAAGCTTTGTTGCTCATGTAGAGGAGCGAGTTGAAGAATTAGATTGCTCCCAGAAAGCGATCATGCAGATGGTCAGCGACCTTTTCGACGACTTTCGAGCTGCTATAGAAGTCGTGCTGGCAGAAGTCGCCGAGATAAACATGAAGATTCCAGAGCCTAAGTCCTTTGGCGGAACTTGCGATGCCAAGGCCCTCGAGAACTTCATCTTTAACGTCGAACAATACTTTCGAGCTATGAGTACAACGACAGAAGAAGCCAAGATCACTTTGGCAACCATGCATCTAGCTGAAGATGCAAAGCTTTGGTGGAGGTCCAAGTATATGGACATCCAAGAGGGTCGATGCACTATAAACACATGGGATGAGTTGAAGAAAGCTCTAAGGGCACAGTTCTTTTCGGAGAATGTTGAGATTCTAGCGAGACGTAAACTGCGAGAGTTGAGACACACTAGAAGGAACCAACCCTCTTCGAGTAGTGGGAACAAACCCACCAGAACAAACTCACCCAAAAGTGGGGGAGGAGGCAGGAATGAAGGAGCAGACCGCAGACCCTATCAGAGGGGTGGAACCAACTGGCGAGGGCAAAACCAACAGAACAACTTCAATCGAGGACCCTTTGTTTGCTTTCTGTGTAAAGGGGCTTATCGCATAAGCGACTGCCTGCATCGAGCTGCCCTGAAGAAACTCCAAGCTACAATAGCCAATGGTCCTGAGGTCGAGAATGAAGTTGTCGAGACAGAGATACCGACCGAAGAGCCTACTGATAACCTCTGCATTGGGGCACTGAAATTTTTGTCGGCTCTCCAGAAGAAAGTAGGGGAAGCAAAAAAACAGCTAAAGAGAGGGCTCATGTATGTGGACGTGTTGGTTCACGGTAAAGCTGCTAAGAGTACTATGGTGGATTCTGGAGCCACACACAACTTCATGGCTGAAATCTAG

Coding sequence (CDS)

ATGTCGGTAGTGAAACAGTTGAACAAGAAACATGTCGAACGACTTGTGCAAATTGAGGAACAACTATTGTTCTTAAGAGAAGTCCCTGATCATGTTCGGTTCTTAGAAAATAGGCTAGAAGAGTTGGCTAGCAAAGCCGATGGAATCGATGCGACAAATGCTCGAATTGACGCATTGCCAGTGAAAGAGTTGTTGGTTAAGGTTGAGACCCTAGAAAACAAAGCTGGGAAAATGACTAGTTTCGAGCGAGAGGACAACTCGACAAGCTTTGTTGCTCATGTAGAGGAGCGAGTTGAAGAATTAGATTGCTCCCAGAAAGCGATCATGCAGATGGTCAGCGACCTTTTCGACGACTTTCGAGCTGCTATAGAAGTCGTGCTGGCAGAAGTCGCCGAGATAAACATGAAGATTCCAGAGCCTAAGTCCTTTGGCGGAACTTGCGATGCCAAGGCCCTCGAGAACTTCATCTTTAACGTCGAACAATACTTTCGAGCTATGAGTACAACGACAGAAGAAGCCAAGATCACTTTGGCAACCATGCATCTAGCTGAAGATGCAAAGCTTTGGTGGAGGTCCAAGTATATGGACATCCAAGAGGGTCGATGCACTATAAACACATGGGATGAGTTGAAGAAAGCTCTAAGGGCACAGTTCTTTTCGGAGAATGTTGAGATTCTAGCGAGACGTAAACTGCGAGAGTTGAGACACACTAGAAGGAACCAACCCTCTTCGAGTAGTGGGAACAAACCCACCAGAACAAACTCACCCAAAAGTGGGGGAGGAGGCAGGAATGAAGGAGCAGACCGCAGACCCTATCAGAGGGGTGGAACCAACTGGCGAGGGCAAAACCAACAGAACAACTTCAATCGAGGACCCTTTGTTTGCTTTCTGTGTAAAGGGGCTTATCGCATAAGCGACTGCCTGCATCGAGCTGCCCTGAAGAAACTCCAAGCTACAATAGCCAATGGTCCTGAGGTCGAGAATGAAGTTGTCGAGACAGAGATACCGACCGAAGAGCCTACTGATAACCTCTGCATTGGGGCACTGAAATTTTTGTCGGCTCTCCAGAAGAAAGTAGGGGAAGCAAAAAAACAGCTAAAGAGAGGGCTCATGTATGTGGACGTGTTGGTTCACGGTAAAGCTGCTAAGAGTACTATGGTGGATTCTGGAGCCACACACAACTTCATGGCTGAAATCTAG

Protein sequence

MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALPVKELLVKVETLENKAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDDFRAAIEVVLAEVAEINMKIPEPKSFGGTCDAKALENFIFNVEQYFRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKALRAQFFSENVEILARRKLRELRHTRRNQPSSSSGNKPTRTNSPKSGGGGRNEGADRRPYQRGGTNWRGQNQQNNFNRGPFVCFLCKGAYRISDCLHRAALKKLQATIANGPEVENEVVETEIPTEEPTDNLCIGALKFLSALQKKVGEAKKQLKRGLMYVDVLVHGKAAKSTMVDSGATHNFMAEI
Homology
BLAST of Moc06g19770 vs. NCBI nr
Match: XP_022155185.1 (uncharacterized protein LOC111022320 [Momordica charantia])

HSP 1 Score: 420.2 bits (1079), Expect = 1.9e-113
Identity = 231/458 (50.44%), Postives = 309/458 (67.47%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS  KQL K H++RLV+IEE+LLFLRE+PD++R++E+RL+E+++KADGID  NARID L 
Sbjct: 1   MSTTKQLGKSHIDRLVEIEERLLFLREIPDNLRYVESRLDEISTKADGIDVVNARIDGLA 60

Query: 61  VKELLVKVETLENKAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDDFR 120
           ++EL+++VETLE+K  +  + ER ++S+S +AH+EERVEE+D S+K I+QMVS+L DDF+
Sbjct: 61  IRELMLRVETLEDKVKRTDNLERGESSSSSIAHMEERVEEIDISKKTIVQMVSELTDDFK 120

Query: 121 AAIEVVLAEVAEINMKIPEPKSFGGTCDAKALENFIFNVEQYFRAMSTTTEEAKITLATM 180
           A ++ + AE+AE+       K F G  DAKALENFIF++EQYF+A ST TEE+K+TLATM
Sbjct: 121 ATVDEMRAEIAELG-----TKPFCGARDAKALENFIFDLEQYFKATSTVTEESKVTLATM 180

Query: 181 HLAEDAKLWWRSKYMDIQEGRCTINTWDELKKALRAQ----------------------- 240
           HLA+DAKLWWR++Y+D+QEG+CTI+TW++LK+ LR+Q                       
Sbjct: 181 HLADDAKLWWRARYVDVQEGKCTIDTWEKLKQELRSQSINFVNYDTGNIRDYVKQFSGLM 240

Query: 241 -------------FFSENVEILARRKLRELR-----------------------HTRRNQ 300
                         F E ++  AR KL E +                         R+ Q
Sbjct: 241 LDIRDMSEKDKVFAFVEGLKPWARAKLYEQKVQDIPTAYATAEQLFNLSSDTSQEQRKTQ 300

Query: 301 PSSSSGNKPTRTNSPKSGGGGRNEGADRRPY-QRGGTNWRGQNQQNNFNRGPFVCFLCKG 360
            SSSSG++P R+ SPK+GG  +N G DRRP+ QRGG  WRG N  NN NR    CF+CKG
Sbjct: 301 ASSSSGHRPARSGSPKNGGADKNGGGDRRPFQQRGGNTWRGPNPPNNNNRTGPSCFICKG 360

Query: 361 AYRISDCLHRAALKKLQATIANGPEVENEVVETEIPTEEPTDNLCIGALKFLSALQKKVG 399
            +R+ +C +RAAL+  QAT+ N   +E E  ET+ P E+  DN  +GALKFLSALQKK  
Sbjct: 361 PHRVYECPNRAALRAFQATLTNDRAIEPEGPETDTPPEDADDNPRMGALKFLSALQKKAE 420

BLAST of Moc06g19770 vs. NCBI nr
Match: KAA0032583.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 392.1 bits (1006), Expect = 5.7e-105
Identity = 217/468 (46.37%), Postives = 295/468 (63.03%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS      K   +RL+++EEQ+L+L EVPD +R+LE+RLEE++ K + IDA   R++  P
Sbjct: 1   MSSSNPSGKAQKDRLIELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  VKELLVKVETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDD 120
           ++EL+ +V+ LE     G+  ++ER D+ST  VAH+EERV+ELD SQK +++M++ + +D
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 FRAAIEVVLAEVAEIN------------------------MKIPEPKSFGGTCDAKALEN 180
           FRA ++VV  E+A++N                        +KIPEPK F G  DAKALEN
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGGAIPVSRVKIPEPKPFCGARDAKALEN 180

Query: 181 FIFNVEQYFRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKAL 240
           +IF++EQYFRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQEGRCTI+TWD LK+ L
Sbjct: 181 YIFDLEQYFRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQEGRCTIDTWDALKREL 240

Query: 241 RAQFFSENVEILARRKLRELRH-------------------------------------- 300
           R+QFF ENVEILARRKLREL+H                                      
Sbjct: 241 RSQFFPENVEILARRKLRELKHTGSIREIEPWAKTKLYEQRVQDLTSAYAAAERLFDLSN 300

Query: 301 ----TRRNQPSSSSGNKPTRTNSPKSGGGGRNEGADRRPYQRG-GTNWRGQNQQNNFNRG 360
               TRR+  SSS G++  R +SPK+ GG R    DRR +Q   G +WRG + QN  NR 
Sbjct: 301 DSQDTRRHPSSSSGGSRNNRPSSPKTTGGDRRFNGDRRSHQSNTGNSWRGSSNQNLSNR- 360

Query: 361 PFVCFLCKGAYRISDCLHRAALKKLQATIANGPEVENEVVETEIPTEEPTDNLCIGALKF 400
           P  CF+CKG +   +C ++ A    QA++ +  + +    E E+   E  DN  +GALKF
Sbjct: 361 PLSCFICKGPHMARECPNKTAFNAFQASLTSDSDNQQSQTEGEVNQTEEVDNPRMGALKF 420

BLAST of Moc06g19770 vs. NCBI nr
Match: KAA0053390.1 (polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 385.2 bits (988), Expect = 6.9e-103
Identity = 211/455 (46.37%), Postives = 290/455 (63.74%), Query Frame = 0

Query: 9   KKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALPVKELLVKV 68
           K   +RLV++EEQ+L+L EVPD +R+LE+RL+E++ K + IDA   R++  P++EL+ +V
Sbjct: 9   KAQKDRLVELEEQMLYLVEVPDSIRYLESRLDEISEKTNTIDAVAGRVEGFPIQELMARV 68

Query: 69  ETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDDFRAAIEVV 128
           + LE     G+  ++ER D+ST  +AH+EERV+ELD SQK +++M++ + +DFRA ++VV
Sbjct: 69  DALETNINIGRTVNYERGDSSTGSIAHIEERVQELDNSQKTLLEMINGMSEDFRATLDVV 128

Query: 129 LAEVAEIN------------------------MKIPEPKSFGGTCDAKALENFIFNVEQY 188
             E+A++N                        +KIPEPK F G  DAKALEN+IF++EQY
Sbjct: 129 RNEIADVNARLSLTMRAMANQAPAEGAIPVSRVKIPEPKPFCGARDAKALENYIFDLEQY 188

Query: 189 FRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKALRAQFFSEN 248
           FRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQEGRCTI+TWD LK+ LR+QFF EN
Sbjct: 189 FRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQEGRCTIDTWDALKRELRSQFFPEN 248

Query: 249 VEILARRKLRELRH--------------------------------------TRRNQPSS 308
           V+ILARRKLREL+H                                      TRR+  SS
Sbjct: 249 VKILARRKLRELKHTGLKPWAKTKLYEQRVQDFTSAYAAAERLFDLSNDSQDTRRHPSSS 308

Query: 309 SSGNKPTRTNSPKSGGGGRNEGADRRPYQRG-GTNWRGQNQQNNFNRGPFVCFLCKGAYR 368
           S G++  R +SPK+ GG R    D R +Q   G +WRG + Q N +  P  CF+CKG + 
Sbjct: 309 SGGSRNNRPSSPKTTGGDRRFNGDCRSHQSNTGNSWRGSSNQ-NLSNSPLSCFICKGPHM 368

Query: 369 ISDCLHRAALKKLQATIANGPEVENEVVETEIPTEEPTDNLCIGALKFLSALQKKVGEAK 399
             +C ++ A    QA++A+  + +    E E+   E  DN  +GALKFLS+LQKKVGE  
Sbjct: 369 ARECPNKTAFNAFQASLASDSDNQQSQTEREVDQTEEVDNPRMGALKFLSSLQKKVGETN 428

BLAST of Moc06g19770 vs. NCBI nr
Match: KAA0032849.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 384.0 bits (985), Expect = 1.5e-102
Identity = 219/495 (44.24%), Postives = 295/495 (59.60%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS      K   +RLV++EEQ+L+L EVPD +R+LE+RLEE++ K + IDA   R++  P
Sbjct: 1   MSSSNPSGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  VKELLVKVETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDD 120
           ++EL+ +V+ LE     G+  ++ER D+ST  VAH+EERV+ELD SQK +++M++ + +D
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 FRAAIEVVLAEVAEIN------------------------MKIPEPKSFGGTCDAKALEN 180
           FRA ++VV  E+A++N                        +KIPEPK F G  DAKALEN
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGGAIPVSRVKIPEPKPFCGARDAKALEN 180

Query: 181 FIFNVEQYFRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKAL 240
           +IF++EQYFRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQ+GRCTI+TWD LK+ L
Sbjct: 181 YIFDLEQYFRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQKGRCTIDTWDALKREL 240

Query: 241 RAQFFSENVEILARRKLRELRH-------------------------------------- 300
           R+QFF ENVEILARRKLREL+H                                      
Sbjct: 241 RSQFFPENVEILARRKLRELKHTGSIREYVKQFAGLMLDIRDMSEKDKVFCFVEGLKPWA 300

Query: 301 -------------------------------TRRNQPSSSSGNKPTRTNSPKSGGGGRNE 360
                                          TRR+  SSS G++  R +SPK+ GG R  
Sbjct: 301 KTKLYEQRVQDLTSAYAAAERLFDLSNDSQDTRRHPSSSSGGSRNNRPSSPKTTGGDRRF 360

Query: 361 GADRRPYQRG-GTNWRGQNQQNNFNRGPFVCFLCKGAYRISDCLHRAALKKLQATIANGP 400
             DRR YQ   G +WRG N QN  NR P  CF+CKG +   +C ++ A    QA++ +  
Sbjct: 361 SGDRRSYQSNTGNSWRGSNNQNLSNR-PLSCFICKGPHMARECPNKTAFNAFQASLTSDS 420

BLAST of Moc06g19770 vs. NCBI nr
Match: TYK03099.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 384.0 bits (985), Expect = 1.5e-102
Identity = 219/495 (44.24%), Postives = 296/495 (59.80%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS    L K   +RLV++EEQ+L+L EVPD +R+LE+RLEE++ K + IDA   R++  P
Sbjct: 1   MSSSNPLGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  VKELLVKVETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDD 120
           ++EL+ +V+ LE     G+  ++ER D+ST  VAH+EERV+ELD SQK +++M++ + +D
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 FRAAIEVVLAEVAEIN------------------------MKIPEPKSFGGTCDAKALEN 180
           FRA ++VV  E+A++N                        +KIPEPK F G  DAKALEN
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGGAIPVSRVKIPEPKPFCGARDAKALEN 180

Query: 181 FIFNVEQYFRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKAL 240
           +IF++EQYFRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQEGRCTI+TWD LK+ L
Sbjct: 181 YIFDLEQYFRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQEGRCTIDTWDALKREL 240

Query: 241 RAQFFSENVEILARRKLRELRH-------------------------------------- 300
           R+QFF ENVEILARRKLREL+H                                      
Sbjct: 241 RSQFFPENVEILARRKLRELKHTGSIREYVKQFAGLMLDIRDMSEKDKVFCFVEGLKPWA 300

Query: 301 -------------------------------TRRNQPSSSSGNKPTRTNSPKSGGGGRNE 360
                                          TRR+  SSS G++  R +SPK+ GG R  
Sbjct: 301 KTKLYEQRVQDLTSAYAAAERLFDLSNDSQDTRRHPSSSSGGSRNNRPSSPKTTGGDRRF 360

Query: 361 GADRRPYQRG-GTNWRGQNQQNNFNRGPFVCFLCKGAYRISDCLHRAALKKLQATIANGP 400
             DRR +Q   G +WRG + QN  NR P  CF+CKG +   +C ++ A    QA++ +  
Sbjct: 361 SGDRRSHQSNTGNSWRGSSNQNLSNR-PLSCFICKGPHMARECPNKTAFNAFQASLTSDS 420

BLAST of Moc06g19770 vs. ExPASy TrEMBL
Match: A0A6J1DLQ6 (uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022320 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 9.4e-114
Identity = 231/458 (50.44%), Postives = 309/458 (67.47%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS  KQL K H++RLV+IEE+LLFLRE+PD++R++E+RL+E+++KADGID  NARID L 
Sbjct: 1   MSTTKQLGKSHIDRLVEIEERLLFLREIPDNLRYVESRLDEISTKADGIDVVNARIDGLA 60

Query: 61  VKELLVKVETLENKAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDDFR 120
           ++EL+++VETLE+K  +  + ER ++S+S +AH+EERVEE+D S+K I+QMVS+L DDF+
Sbjct: 61  IRELMLRVETLEDKVKRTDNLERGESSSSSIAHMEERVEEIDISKKTIVQMVSELTDDFK 120

Query: 121 AAIEVVLAEVAEINMKIPEPKSFGGTCDAKALENFIFNVEQYFRAMSTTTEEAKITLATM 180
           A ++ + AE+AE+       K F G  DAKALENFIF++EQYF+A ST TEE+K+TLATM
Sbjct: 121 ATVDEMRAEIAELG-----TKPFCGARDAKALENFIFDLEQYFKATSTVTEESKVTLATM 180

Query: 181 HLAEDAKLWWRSKYMDIQEGRCTINTWDELKKALRAQ----------------------- 240
           HLA+DAKLWWR++Y+D+QEG+CTI+TW++LK+ LR+Q                       
Sbjct: 181 HLADDAKLWWRARYVDVQEGKCTIDTWEKLKQELRSQSINFVNYDTGNIRDYVKQFSGLM 240

Query: 241 -------------FFSENVEILARRKLRELR-----------------------HTRRNQ 300
                         F E ++  AR KL E +                         R+ Q
Sbjct: 241 LDIRDMSEKDKVFAFVEGLKPWARAKLYEQKVQDIPTAYATAEQLFNLSSDTSQEQRKTQ 300

Query: 301 PSSSSGNKPTRTNSPKSGGGGRNEGADRRPY-QRGGTNWRGQNQQNNFNRGPFVCFLCKG 360
            SSSSG++P R+ SPK+GG  +N G DRRP+ QRGG  WRG N  NN NR    CF+CKG
Sbjct: 301 ASSSSGHRPARSGSPKNGGADKNGGGDRRPFQQRGGNTWRGPNPPNNNNRTGPSCFICKG 360

Query: 361 AYRISDCLHRAALKKLQATIANGPEVENEVVETEIPTEEPTDNLCIGALKFLSALQKKVG 399
            +R+ +C +RAAL+  QAT+ N   +E E  ET+ P E+  DN  +GALKFLSALQKK  
Sbjct: 361 PHRVYECPNRAALRAFQATLTNDRAIEPEGPETDTPPEDADDNPRMGALKFLSALQKKAE 420

BLAST of Moc06g19770 vs. ExPASy TrEMBL
Match: A0A5A7SQC7 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43053G00490 PE=4 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 2.7e-105
Identity = 217/468 (46.37%), Postives = 295/468 (63.03%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS      K   +RL+++EEQ+L+L EVPD +R+LE+RLEE++ K + IDA   R++  P
Sbjct: 1   MSSSNPSGKAQKDRLIELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  VKELLVKVETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDD 120
           ++EL+ +V+ LE     G+  ++ER D+ST  VAH+EERV+ELD SQK +++M++ + +D
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 FRAAIEVVLAEVAEIN------------------------MKIPEPKSFGGTCDAKALEN 180
           FRA ++VV  E+A++N                        +KIPEPK F G  DAKALEN
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGGAIPVSRVKIPEPKPFCGARDAKALEN 180

Query: 181 FIFNVEQYFRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKAL 240
           +IF++EQYFRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQEGRCTI+TWD LK+ L
Sbjct: 181 YIFDLEQYFRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQEGRCTIDTWDALKREL 240

Query: 241 RAQFFSENVEILARRKLRELRH-------------------------------------- 300
           R+QFF ENVEILARRKLREL+H                                      
Sbjct: 241 RSQFFPENVEILARRKLRELKHTGSIREIEPWAKTKLYEQRVQDLTSAYAAAERLFDLSN 300

Query: 301 ----TRRNQPSSSSGNKPTRTNSPKSGGGGRNEGADRRPYQRG-GTNWRGQNQQNNFNRG 360
               TRR+  SSS G++  R +SPK+ GG R    DRR +Q   G +WRG + QN  NR 
Sbjct: 301 DSQDTRRHPSSSSGGSRNNRPSSPKTTGGDRRFNGDRRSHQSNTGNSWRGSSNQNLSNR- 360

Query: 361 PFVCFLCKGAYRISDCLHRAALKKLQATIANGPEVENEVVETEIPTEEPTDNLCIGALKF 400
           P  CF+CKG +   +C ++ A    QA++ +  + +    E E+   E  DN  +GALKF
Sbjct: 361 PLSCFICKGPHMARECPNKTAFNAFQASLTSDSDNQQSQTEGEVNQTEEVDNPRMGALKF 420

BLAST of Moc06g19770 vs. ExPASy TrEMBL
Match: A0A5A7UDW8 (Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold428G00550 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 3.4e-103
Identity = 211/455 (46.37%), Postives = 290/455 (63.74%), Query Frame = 0

Query: 9   KKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALPVKELLVKV 68
           K   +RLV++EEQ+L+L EVPD +R+LE+RL+E++ K + IDA   R++  P++EL+ +V
Sbjct: 9   KAQKDRLVELEEQMLYLVEVPDSIRYLESRLDEISEKTNTIDAVAGRVEGFPIQELMARV 68

Query: 69  ETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDDFRAAIEVV 128
           + LE     G+  ++ER D+ST  +AH+EERV+ELD SQK +++M++ + +DFRA ++VV
Sbjct: 69  DALETNINIGRTVNYERGDSSTGSIAHIEERVQELDNSQKTLLEMINGMSEDFRATLDVV 128

Query: 129 LAEVAEIN------------------------MKIPEPKSFGGTCDAKALENFIFNVEQY 188
             E+A++N                        +KIPEPK F G  DAKALEN+IF++EQY
Sbjct: 129 RNEIADVNARLSLTMRAMANQAPAEGAIPVSRVKIPEPKPFCGARDAKALENYIFDLEQY 188

Query: 189 FRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKALRAQFFSEN 248
           FRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQEGRCTI+TWD LK+ LR+QFF EN
Sbjct: 189 FRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQEGRCTIDTWDALKRELRSQFFPEN 248

Query: 249 VEILARRKLRELRH--------------------------------------TRRNQPSS 308
           V+ILARRKLREL+H                                      TRR+  SS
Sbjct: 249 VKILARRKLRELKHTGLKPWAKTKLYEQRVQDFTSAYAAAERLFDLSNDSQDTRRHPSSS 308

Query: 309 SSGNKPTRTNSPKSGGGGRNEGADRRPYQRG-GTNWRGQNQQNNFNRGPFVCFLCKGAYR 368
           S G++  R +SPK+ GG R    D R +Q   G +WRG + Q N +  P  CF+CKG + 
Sbjct: 309 SGGSRNNRPSSPKTTGGDRRFNGDCRSHQSNTGNSWRGSSNQ-NLSNSPLSCFICKGPHM 368

Query: 369 ISDCLHRAALKKLQATIANGPEVENEVVETEIPTEEPTDNLCIGALKFLSALQKKVGEAK 399
             +C ++ A    QA++A+  + +    E E+   E  DN  +GALKFLS+LQKKVGE  
Sbjct: 369 ARECPNKTAFNAFQASLASDSDNQQSQTEREVDQTEEVDNPRMGALKFLSSLQKKVGETN 428

BLAST of Moc06g19770 vs. ExPASy TrEMBL
Match: A0A5D3DQ20 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007440 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 7.5e-103
Identity = 219/495 (44.24%), Postives = 296/495 (59.80%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS      K   +RLV++EEQ+L+L EVPD +R+LE+RLEE++ K + IDA   R++  P
Sbjct: 1   MSSSNPSGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  VKELLVKVETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDD 120
           ++EL+ +V+ LE     G+  ++ER D+ST  VAH+EERV+ELD SQK +++M++ + +D
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 FRAAIEVVLAEVAEIN------------------------MKIPEPKSFGGTCDAKALEN 180
           FRA ++VV  E+A++N                        +KIPEPK F G  DAKALEN
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGGAIPVSRVKIPEPKPFCGARDAKALEN 180

Query: 181 FIFNVEQYFRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKAL 240
           +IF++EQYFRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQEGRCTI+TWD LK+ L
Sbjct: 181 YIFDLEQYFRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQEGRCTIDTWDALKREL 240

Query: 241 RAQFFSENVEILARRKLRELRH-------------------------------------- 300
           R+QFF ENVEILARRKLREL+H                                      
Sbjct: 241 RSQFFPENVEILARRKLRELKHTGSIREYVKQFAGLMLDIRDMSEKDKVFCFVEGLKPWA 300

Query: 301 -------------------------------TRRNQPSSSSGNKPTRTNSPKSGGGGRNE 360
                                          TRR+  SSS G++  R +SPK+ GG R  
Sbjct: 301 KTKLYEQRVQDLTSAYAAAERLFDLSNDSQDTRRHPSSSSGGSRNNRPSSPKTTGGDRRF 360

Query: 361 GADRRPYQRG-GTNWRGQNQQNNFNRGPFVCFLCKGAYRISDCLHRAALKKLQATIANGP 400
             DRR +Q   G +WRG + QN  NR P  CF+CKG +   +C ++ A    QA++A+  
Sbjct: 361 NGDRRSHQSNTGNSWRGSSNQNLSNR-PLSCFICKGPHMARECPNKTAFNAFQASLASDS 420

BLAST of Moc06g19770 vs. ExPASy TrEMBL
Match: A0A5D3BYE6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00260 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 7.5e-103
Identity = 219/495 (44.24%), Postives = 296/495 (59.80%), Query Frame = 0

Query: 1   MSVVKQLNKKHVERLVQIEEQLLFLREVPDHVRFLENRLEELASKADGIDATNARIDALP 60
           MS    L K   +RLV++EEQ+L+L EVPD +R+LE+RLEE++ K + IDA   R++  P
Sbjct: 1   MSSSNPLGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  VKELLVKVETLEN--KAGKMTSFEREDNSTSFVAHVEERVEELDCSQKAIMQMVSDLFDD 120
           ++EL+ +V+ LE     G+  ++ER D+ST  VAH+EERV+ELD SQK +++M++ + +D
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 FRAAIEVVLAEVAEIN------------------------MKIPEPKSFGGTCDAKALEN 180
           FRA ++VV  E+A++N                        +KIPEPK F G  DAKALEN
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGGAIPVSRVKIPEPKPFCGARDAKALEN 180

Query: 181 FIFNVEQYFRAMSTTTEEAKITLATMHLAEDAKLWWRSKYMDIQEGRCTINTWDELKKAL 240
           +IF++EQYFRA +T TEEAK+TLATMHL+EDAKLWWRS+++DIQEGRCTI+TWD LK+ L
Sbjct: 181 YIFDLEQYFRATNTVTEEAKVTLATMHLSEDAKLWWRSRFVDIQEGRCTIDTWDALKREL 240

Query: 241 RAQFFSENVEILARRKLRELRH-------------------------------------- 300
           R+QFF ENVEILARRKLREL+H                                      
Sbjct: 241 RSQFFPENVEILARRKLRELKHTGSIREYVKQFAGLMLDIRDMSEKDKVFCFVEGLKPWA 300

Query: 301 -------------------------------TRRNQPSSSSGNKPTRTNSPKSGGGGRNE 360
                                          TRR+  SSS G++  R +SPK+ GG R  
Sbjct: 301 KTKLYEQRVQDLTSAYAAAERLFDLSNDSQDTRRHPSSSSGGSRNNRPSSPKTTGGDRRF 360

Query: 361 GADRRPYQRG-GTNWRGQNQQNNFNRGPFVCFLCKGAYRISDCLHRAALKKLQATIANGP 400
             DRR +Q   G +WRG + QN  NR P  CF+CKG +   +C ++ A    QA++ +  
Sbjct: 361 SGDRRSHQSNTGNSWRGSSNQNLSNR-PLSCFICKGPHMARECPNKTAFNAFQASLTSDS 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155185.11.9e-11350.44uncharacterized protein LOC111022320 [Momordica charantia][more]
KAA0032583.15.7e-10546.37reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0053390.16.9e-10346.37polyprotein [Cucumis melo var. makuwa][more]
KAA0032849.11.5e-10244.24reverse transcriptase [Cucumis melo var. makuwa][more]
TYK03099.11.5e-10244.24reverse transcriptase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DLQ69.4e-11450.44uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5A7SQC72.7e-10546.37Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43... [more]
A0A5A7UDW83.4e-10346.37Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold428G00550 PE... [more]
A0A5D3DQ207.5e-10344.24Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3BYE67.5e-10344.24Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 32..52
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..285
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 240..260
NoneNo IPR availablePANTHERPTHR34482:SF18RETROTRANSPOSON GAG DOMAIN, ASPARTIC PEPTIDASE DOMAIN PROTEIN-RELATEDcoord: 85..238
NoneNo IPR availablePANTHERPTHR34482DNA DAMAGE-INDUCIBLE PROTEIN 1-LIKEcoord: 85..238
IPR005162Retrotransposon gag domainPFAMPF03732Retrotrans_gagcoord: 177..236
e-value: 5.4E-7
score: 29.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g19770.1Moc06g19770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:0016787 hydrolase activity