Moc06g14500 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g14500
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description(1->3)-beta-glucan endohydrolase
Locationchr6: 11391347 .. 11394737 (+)
RNA-Seq ExpressionMoc06g14500
SyntenyMoc06g14500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGAAGAACAGTGGTAAAATGGGTTGGTTCTTCAAAGCTTTCGTGGGTTTGGTTTCGTTTTTTTCACTGGTGTGTTCTGTGGCGGCCATCGGAGCCAACTGGGGAACTCAGGCGACGCATCCGCTGCCGCCGGAGACGGTGGTCGGAATGCTGAGGGACAACCAGATTCAGAAAGTGAAGCTCTTTGATGCTGATTATGGAACTCTCAGAGCTCTGGGTAAGACTGGGATTGAGGTCATGGTTGGGATTCCAAATGATATGCTCTATTCTCTTGCAAGTAGTGTGAAAGCAGCTGAAAAATGGGTTTCTAAAAATGTCTCTGTTCATATTTCCACCAATAATGTTAACATCAGGTGAGTTTTTTTTTTTTTTTCCTTAGTTGTTTCCTTCATGGCTCTGCTTGTTTTTGTTTTGTTTTGTGTGTTTTTGGCAAATTGGGTGACTTTTTCCCCCTTTTTCCAATAGTGTTGCTTTTTATGTCTTAATCAGTCGTGTAATTGTTAAGAAGTTAATGTTTTGATTTGTTTCCTTCTTTCTCTTTTGGTTAATTAATTGTTGAAAAGTTGGATGTCACATCTTAATGATTGAGAAGTGGGAAAGTTCTGTGATTAGTGCTTGTTTGCTATGGTGGCTTGTTCTTCAATCTTGTGAAGATTTTTCATCTTTTTGAAGGATCGAGTCTTGGATTTTCAGGGGAAACAAGTTTTGTATTGATTAGGGCAAATCCGAACTACAACGGTTGTTTATTGTTCTTGTTCTAATATAGAGAGTAGGTTGCCTTTGCCGTGTTTTTTGAAGAAATGATGTGGCGGGCAAAGGGTAGAACAAAGCGGACAGTTGGATAAAATAAAGTGGCACGTTTAAATGCATCCTCTTTTTAAAACAGCATATCAAGTCACATTTTAAAATTCAAAGACGTAATTGAAACTGTTTAGAGATTAGGGACTTGATAAGCCCCTCGTCAAAGTTCAAGGACTAAATTTGTAATTTTAACACGTTAGGCAAGACTTGCTTGCTTTGAAGAACTAGATTATGTTTTGTTTTTCCTAAAAATGGTCTCCTTCATATTATTTATTCATTTCTTTGTTTATCTTTGCAAGTTAGCACTGATAATGAATTTTTTTTGTTCAAAACCAAAGGTTTAGTAGTCAAATATTCATGATATGTATTAAAAAATACACACACACACTTCTTTTACATTATCTGAAATCATGTTTCACTAGAACCAGTTCTCTTTGCTTCTTTTTTGTGGTAACATTCTAGGACAGAGTTTTCAAAACTATAAGTCTTAGAGTTGAGATTTTGATCATATCATAGTTGGCATGAACAAATGAGATTATCAAGTCCCTCGCCTCCATTTTCCCTCCTAGTTGTAGCTTTCTTTGAGTTTTGACTGCTTTTTTTGATATATGGGAACTATACACCTGTCGAGCTTATTCAAACGACAAACTCGAACACCTTGGCTGAAGTCGTCATAGTTTTTTAGGAATCTTGATCGGCTGCCATTGAAACTTCATCCTCCAGTGGGTACGAAACTGGGTCGAAATGGGGTCTCAATTATCTTTTTGCCATAACATCCTTCGTTTCGACATAAGTTAGTACAAGATCACACTATAATAGGGCCATGATCAGTACACAGCAATCTCCTCTGCTTTTGCACACTTTAACAGGGTTTAGCCTTGTGAAGGGATTGATTCTATACACTTTCAGTTAAAATTAAGTGAAGGGGCTCTCTCCCAATGAATTTTACTCACTTCTTTCTTGCAATCTATCTTCTCTGATGGGGCTAACAGAGGATCATAGTTTGGAGCTACCATTTTCACTAGAGTTGGAGGGATCCGGTTTATCGGTGGATGCTCACAACCTTCGGAACTACTTTCTCGTTTTTCACCATCTGAAACACTTTCTATCGGTGCTTATTATTTGTAAGATTTTGAATCAAGAAGCAGAACTAAGATGACTGTTCTTTGAACCATGTATGAATTTTAAGCTTTGTGGAAGCATGGGAATAATGTGGAATTTTCTTAAACAAATTCAGATCACATCATTTAGTTACACGAGGATGAGGAGATGAATGGTTTGTGGTTTGAAATTGTACTGAATACTATATGATTATACAAGACCGAATAACGGACTTGCTTTAACAATTGTACTTGTTTGATGGTCGATATTCATATGTAATGTTCGTGTAGTACAAAGCAGGAAAATACCCCTTTAAATGATCATGCATTCATTAATTTCTTTCAAGTCACCAAGTTGATTATGAATTTTCAGATATGTTGCTGTAGGGAATGAGCCTTTCTTGTCAACTTACAATGGAAGCTTCCTTAAAACAACCTTTCCTGCTCTGCAGAATGTTCAGTCTGCTCTGATAAAAGCCGGTCTCGCCAACCAAGTAAAGGTAACCGTTCCCCTGAATGCTGATGTCTACGCGAGCTCCACCACTTTTCCATCTGGTGGCGACTTCCGGACTGACATACATGATTTGATGATCAACATCGTCAAGTTCTTGAGCGACTCTGGTTCTCCGTTCACAGTGAACATTTACCCCTTCATCAGTCTCTATAGCGACCCCAACTTTCCTGTTGAATATGCTTTCTTTGATGGCAATGCATCTCCCATAGTCGATGGTGGGACATCATATTTCAACATGTTCGATGCAAATTATGACACCCTTGTGTGGGCGCTTCAGAAGAATGGCTTTGGAAACTTACCGATCATAGTCGGGGAGATCGGTTGGCCTACGGACGGGGATCGAAATGCCAACCCCATCTATGCTCAACGGTTCAACCAAGGCTTCATGTCACATATACTAGGTGGAAAAGGAACACCGATGAGGCCAGGTCCAATCGACACCTATTTATTCAGCCTAATCGACGAAGACGCGAAGAGCATTGATCCAGGGAACTTCGAACGCCATTGGGGCATATTCTACTACGATGGTCGACCAAAATACCAGCTCAACCTCGGAACTTCAAACAACGGGTCCTTAGTCGCAGCCAGAGGCGTGCGTTATCTAGAAAAAAAGTGGTGCGTGATGAAGCGATCAGCCCGCCTCGAGGATTCGCAAGTCGCACCGAGCGTAAGCTATGCGTGTTACCACGCCGATTGCACCAGCCTCGGGTACGGCACGTCGTGTTCAAGCGTAGATGCAAGGTTGAACATTTCATATGCATTCAACAGCTATTACCAGAGGAACAATCAGGGAGAGGATGACTGCAAGTTCTCAGGGTTGGCAGAAGTGACAAATAATGATCCAAGTTTTGGGAATTGCAGGTTTGATATAATGATAGAGCCATATTATGGAGCTGCAGAAGGAAGAGGGTGCTGTTTCAAAAAGCACTTGATGTTGCTTTCTGGGCTGCTTCTTGTGTTGACTTTTCTGTGA

mRNA sequence

ATGTTGAAGAACAGTGGTAAAATGGGTTGGTTCTTCAAAGCTTTCGTGGGTTTGGTTTCGTTTTTTTCACTGGTGTGTTCTGTGGCGGCCATCGGAGCCAACTGGGGAACTCAGGCGACGCATCCGCTGCCGCCGGAGACGGTGGTCGGAATGCTGAGGGACAACCAGATTCAGAAAGTGAAGCTCTTTGATGCTGATTATGGAACTCTCAGAGCTCTGGGTAAGACTGGGATTGAGGTCATGGTTGGGATTCCAAATGATATGCTCTATTCTCTTGCAAGTAGTGTGAAAGCAGCTGAAAAATGGGTTTCTAAAAATGTCTCTGTTCATATTTCCACCAATAATGTTAACATCAGATATGTTGCTGTAGGGAATGAGCCTTTCTTGTCAACTTACAATGGAAGCTTCCTTAAAACAACCTTTCCTGCTCTGCAGAATGTTCAGTCTGCTCTGATAAAAGCCGGTCTCGCCAACCAAGTAAAGGTAACCGTTCCCCTGAATGCTGATGTCTACGCGAGCTCCACCACTTTTCCATCTGGTGGCGACTTCCGGACTGACATACATGATTTGATGATCAACATCGTCAAGTTCTTGAGCGACTCTGGTTCTCCGTTCACAGTGAACATTTACCCCTTCATCAGTCTCTATAGCGACCCCAACTTTCCTGTTGAATATGCTTTCTTTGATGGCAATGCATCTCCCATAGTCGATGGTGGGACATCATATTTCAACATGTTCGATGCAAATTATGACACCCTTGTGTGGGCGCTTCAGAAGAATGGCTTTGGAAACTTACCGATCATAGTCGGGGAGATCGGTTGGCCTACGGACGGGGATCGAAATGCCAACCCCATCTATGCTCAACGGTTCAACCAAGGCTTCATGTCACATATACTAGGTGGAAAAGGAACACCGATGAGGCCAGGTCCAATCGACACCTATTTATTCAGCCTAATCGACGAAGACGCGAAGAGCATTGATCCAGGGAACTTCGAACGCCATTGGGGCATATTCTACTACGATGGTCGACCAAAATACCAGCTCAACCTCGGAACTTCAAACAACGGGTCCTTAGTCGCAGCCAGAGGCGTGCGTTATCTAGAAAAAAAGTGGTGCGTGATGAAGCGATCAGCCCGCCTCGAGGATTCGCAAGTCGCACCGAGCGTAAGCTATGCGTGTTACCACGCCGATTGCACCAGCCTCGGGTACGGCACGTCGTGTTCAAGCGTAGATGCAAGGTTGAACATTTCATATGCATTCAACAGCTATTACCAGAGGAACAATCAGGGAGAGGATGACTGCAAGTTCTCAGGGTTGGCAGAAGTGACAAATAATGATCCAAGTTTTGGGAATTGCAGGTTTGATATAATGATAGAGCCATATTATGGAGCTGCAGAAGGAAGAGGGTGCTGTTTCAAAAAGCACTTGATGTTGCTTTCTGGGCTGCTTCTTGTGTTGACTTTTCTGTGA

Coding sequence (CDS)

ATGTTGAAGAACAGTGGTAAAATGGGTTGGTTCTTCAAAGCTTTCGTGGGTTTGGTTTCGTTTTTTTCACTGGTGTGTTCTGTGGCGGCCATCGGAGCCAACTGGGGAACTCAGGCGACGCATCCGCTGCCGCCGGAGACGGTGGTCGGAATGCTGAGGGACAACCAGATTCAGAAAGTGAAGCTCTTTGATGCTGATTATGGAACTCTCAGAGCTCTGGGTAAGACTGGGATTGAGGTCATGGTTGGGATTCCAAATGATATGCTCTATTCTCTTGCAAGTAGTGTGAAAGCAGCTGAAAAATGGGTTTCTAAAAATGTCTCTGTTCATATTTCCACCAATAATGTTAACATCAGATATGTTGCTGTAGGGAATGAGCCTTTCTTGTCAACTTACAATGGAAGCTTCCTTAAAACAACCTTTCCTGCTCTGCAGAATGTTCAGTCTGCTCTGATAAAAGCCGGTCTCGCCAACCAAGTAAAGGTAACCGTTCCCCTGAATGCTGATGTCTACGCGAGCTCCACCACTTTTCCATCTGGTGGCGACTTCCGGACTGACATACATGATTTGATGATCAACATCGTCAAGTTCTTGAGCGACTCTGGTTCTCCGTTCACAGTGAACATTTACCCCTTCATCAGTCTCTATAGCGACCCCAACTTTCCTGTTGAATATGCTTTCTTTGATGGCAATGCATCTCCCATAGTCGATGGTGGGACATCATATTTCAACATGTTCGATGCAAATTATGACACCCTTGTGTGGGCGCTTCAGAAGAATGGCTTTGGAAACTTACCGATCATAGTCGGGGAGATCGGTTGGCCTACGGACGGGGATCGAAATGCCAACCCCATCTATGCTCAACGGTTCAACCAAGGCTTCATGTCACATATACTAGGTGGAAAAGGAACACCGATGAGGCCAGGTCCAATCGACACCTATTTATTCAGCCTAATCGACGAAGACGCGAAGAGCATTGATCCAGGGAACTTCGAACGCCATTGGGGCATATTCTACTACGATGGTCGACCAAAATACCAGCTCAACCTCGGAACTTCAAACAACGGGTCCTTAGTCGCAGCCAGAGGCGTGCGTTATCTAGAAAAAAAGTGGTGCGTGATGAAGCGATCAGCCCGCCTCGAGGATTCGCAAGTCGCACCGAGCGTAAGCTATGCGTGTTACCACGCCGATTGCACCAGCCTCGGGTACGGCACGTCGTGTTCAAGCGTAGATGCAAGGTTGAACATTTCATATGCATTCAACAGCTATTACCAGAGGAACAATCAGGGAGAGGATGACTGCAAGTTCTCAGGGTTGGCAGAAGTGACAAATAATGATCCAAGTTTTGGGAATTGCAGGTTTGATATAATGATAGAGCCATATTATGGAGCTGCAGAAGGAAGAGGGTGCTGTTTCAAAAAGCACTTGATGTTGCTTTCTGGGCTGCTTCTTGTGTTGACTTTTCTGTGA

Protein sequence

MLKNSGKMGWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGRGCCFKKHLMLLSGLLLVLTFL
Homology
BLAST of Moc06g14500 vs. NCBI nr
Match: XP_022157073.1 (glucan endo-1,3-beta-glucosidase 6 [Momordica charantia])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.0e-290
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MLKNSGKMGWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKV 60
           MLKNSGKMGWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKV
Sbjct: 1   MLKNSGKMGWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKV 60

Query: 61  KLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRY 120
           KLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRY
Sbjct: 61  KLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRY 120

Query: 121 VAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSG 180
           VAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSG
Sbjct: 121 VAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSG 180

Query: 181 GDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGT 240
           GDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGT
Sbjct: 181 GDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGT 240

Query: 241 SYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILG 300
           SYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILG
Sbjct: 241 SYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILG 300

Query: 301 GKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVA 360
           GKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVA
Sbjct: 301 GKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVA 360

Query: 361 ARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAF 420
           ARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAF
Sbjct: 361 ARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAF 420

Query: 421 NSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGRGCCFKKHLMLLS 480
           NSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGRGCCFKKHLMLLS
Sbjct: 421 NSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGRGCCFKKHLMLLS 480

Query: 481 GLLLVLTFL 490
           GLLLVLTFL
Sbjct: 481 GLLLVLTFL 489

BLAST of Moc06g14500 vs. NCBI nr
Match: XP_038879688.1 (glucan endo-1,3-beta-glucosidase 6 [Benincasa hispida])

HSP 1 Score: 878.6 bits (2269), Expect = 2.4e-251
Identity = 423/477 (88.68%), Postives = 445/477 (93.29%), Query Frame = 0

Query: 11  FFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTL 70
           F + FV LV  FS+VCSVAAIGANWGTQ++HPLPPETVVGMLRDNQIQKVKLFDADYGTL
Sbjct: 11  FLRCFVVLVVLFSVVCSVAAIGANWGTQSSHPLPPETVVGMLRDNQIQKVKLFDADYGTL 70

Query: 71  RALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPFLS 130
           RALGKTGIEVMVGIPNDML + A+S KAAEKWV+KNVSVH + NNVNIRYVAVGNEPFL+
Sbjct: 71  RALGKTGIEVMVGIPNDMLSTFANSEKAAEKWVAKNVSVHFNDNNVNIRYVAVGNEPFLA 130

Query: 131 TYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIHDL 190
           TYNGSFL TTFPAL+N+Q ALIKA L NQVKVT PLNADVYASST++PSGGDFRTDIHDL
Sbjct: 131 TYNGSFLSTTFPALRNIQRALIKANLGNQVKVTCPLNADVYASSTSYPSGGDFRTDIHDL 190

Query: 191 MINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDANY 250
           M+ IVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDG T+YFNMFDANY
Sbjct: 191 MLAIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGQTTYFNMFDANY 250

Query: 251 DTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGP 310
           DTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGP
Sbjct: 251 DTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGP 310

Query: 311 IDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRYLEKK 370
           ID YLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQL+LG SNNGSLV ARGV YLE+K
Sbjct: 311 IDAYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLSLGNSNNGSLVGARGVHYLERK 370

Query: 371 WCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNNQG 430
           WCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISYAFNSYYQR+NQ 
Sbjct: 371 WCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISYAFNSYYQRSNQA 430

Query: 431 EDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLMLLSGLLLVL 487
           ED CKFSGL+ VTNNDPSFGNCRFDIMIEPYYG AEGR GCCF + LMLLSGL+LVL
Sbjct: 431 EDACKFSGLSTVTNNDPSFGNCRFDIMIEPYYGGAEGRSGCCFTRLLMLLSGLVLVL 487

BLAST of Moc06g14500 vs. NCBI nr
Match: XP_011660196.1 (glucan endo-1,3-beta-glucosidase 6 [Cucumis sativus] >KGN66628.1 hypothetical protein Csa_007662 [Cucumis sativus])

HSP 1 Score: 869.0 bits (2244), Expect = 1.9e-248
Identity = 423/485 (87.22%), Postives = 446/485 (91.96%), Query Frame = 0

Query: 5   SGKMG--WFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKL 64
           + KMG  WF +  V LV   S+VCSV AIGANWGTQ++HPLPPETVVGMLRDNQIQKVKL
Sbjct: 4   NSKMGCCWFLRCIVVLVLLLSVVCSVKAIGANWGTQSSHPLPPETVVGMLRDNQIQKVKL 63

Query: 65  FDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVA 124
           FDADYGTLRALGKTGIEVMVGIPNDML + AS+ KAAEKWVSKNVSVHIS NNVNIRYVA
Sbjct: 64  FDADYGTLRALGKTGIEVMVGIPNDMLSTFASNEKAAEKWVSKNVSVHISENNVNIRYVA 123

Query: 125 VGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGD 184
           VGNEPFL+TYNGSFL TTFPAL+NVQ ALIKA L NQVKVT PLNADVYAS+TTFPSGGD
Sbjct: 124 VGNEPFLATYNGSFLSTTFPALRNVQKALIKANLGNQVKVTCPLNADVYASTTTFPSGGD 183

Query: 185 FRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSY 244
           FR+DIHDLM++IVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDG T+Y
Sbjct: 184 FRSDIHDLMLDIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGQTTY 243

Query: 245 FNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGK 304
           FNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGK
Sbjct: 244 FNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGK 303

Query: 305 GTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAAR 364
           GTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQL+LG S NG+LV AR
Sbjct: 304 GTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLSLGNS-NGTLVGAR 363

Query: 365 GVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNS 424
           GV YLE+KWCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISYAFNS
Sbjct: 364 GVHYLERKWCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISYAFNS 423

Query: 425 YYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLMLLSG 484
           YYQRNNQ ED CKFSGL+ VTNNDPSFG+CRFDIMIEPYYG AEGR G CF + LMLL G
Sbjct: 424 YYQRNNQAEDACKFSGLSTVTNNDPSFGSCRFDIMIEPYYGGAEGRSGFCFTRLLMLLFG 483

Query: 485 LLLVL 487
            +LV+
Sbjct: 484 FVLVM 487

BLAST of Moc06g14500 vs. NCBI nr
Match: KAG7022177.1 (Glucan endo-1,3-beta-glucosidase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 862.8 bits (2228), Expect = 1.4e-246
Identity = 417/484 (86.16%), Postives = 446/484 (92.15%), Query Frame = 0

Query: 5   SGKMG-WFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLF 64
           + KMG  F    VGL+S  S+VCSV AIGANWGTQ++HPLPPETVVGMLRDNQIQKVKLF
Sbjct: 3   NSKMGCCFLHGSVGLLSLISMVCSVVAIGANWGTQSSHPLPPETVVGMLRDNQIQKVKLF 62

Query: 65  DADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAV 124
           DADYGTLRALGKTGIEVMVGIPNDML +LA+S KAAEKWVSKNVSVHIS NNVNIRYVAV
Sbjct: 63  DADYGTLRALGKTGIEVMVGIPNDMLSTLANSEKAAEKWVSKNVSVHISDNNVNIRYVAV 122

Query: 125 GNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDF 184
           GNEPFLSTYNGSFL TTFPAL+N+Q AL+KA L NQVKVTVPLNADVY SSTTFPSGG+F
Sbjct: 123 GNEPFLSTYNGSFLSTTFPALRNIQRALLKANLGNQVKVTVPLNADVYESSTTFPSGGNF 182

Query: 185 RTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYF 244
           R DIHDLM++IVKFLSD+GSPFTVNIYPFISLYS PNFPVEYAFFDGNASP+VDG TSYF
Sbjct: 183 RADIHDLMLDIVKFLSDTGSPFTVNIYPFISLYSTPNFPVEYAFFDGNASPLVDGQTSYF 242

Query: 245 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 304
           NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG
Sbjct: 243 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 302

Query: 305 TPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARG 364
           TPMRPGPID YLFSLIDED+KSIDPGNFERHWG+FYYDG+PKYQL+LG SNNGSLV A+G
Sbjct: 303 TPMRPGPIDAYLFSLIDEDSKSIDPGNFERHWGVFYYDGQPKYQLSLGNSNNGSLVGAKG 362

Query: 365 VRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSY 424
           VRYLE+KWCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISYAFNSY
Sbjct: 363 VRYLERKWCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISYAFNSY 422

Query: 425 YQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLMLLSGL 484
           YQR+NQ ED CKFSGL+ VTN+DPSFG+CRFDIMIEPY G AEGR GC FK+ L L+SG 
Sbjct: 423 YQRSNQAEDACKFSGLSTVTNSDPSFGSCRFDIMIEPYMGGAEGRSGCRFKRLLALVSGF 482

Query: 485 LLVL 487
           +LVL
Sbjct: 483 VLVL 486

BLAST of Moc06g14500 vs. NCBI nr
Match: XP_023520642.1 (glucan endo-1,3-beta-glucosidase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 858.2 bits (2216), Expect = 3.4e-245
Identity = 417/484 (86.16%), Postives = 443/484 (91.53%), Query Frame = 0

Query: 5   SGKMG-WFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLF 64
           + KMG  F    VGL+S  S+VCSV AIGANWGTQ++HPLPPETVVGMLRDNQIQKVKLF
Sbjct: 3   NSKMGCCFLYGSVGLLSLISMVCSVVAIGANWGTQSSHPLPPETVVGMLRDNQIQKVKLF 62

Query: 65  DADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAV 124
           DADYGTLRALGKTGIEVMVGIPNDML +LA+S KAAEKWVSKNVSVHIS NNVNIRYVAV
Sbjct: 63  DADYGTLRALGKTGIEVMVGIPNDMLSTLANSEKAAEKWVSKNVSVHISDNNVNIRYVAV 122

Query: 125 GNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDF 184
           GNEPFLSTYNGSFL TTFPALQN+Q AL+KA L NQVKVTVPLNADVY SSTTFPSGG+F
Sbjct: 123 GNEPFLSTYNGSFLSTTFPALQNIQRALLKANLGNQVKVTVPLNADVYESSTTFPSGGNF 182

Query: 185 RTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYF 244
           R DIHDLM+ IVKFLSD+GSPFTVNIYPFISLYS PNFPVEYAFFDGNASP+VDG TSYF
Sbjct: 183 RADIHDLMLAIVKFLSDTGSPFTVNIYPFISLYSTPNFPVEYAFFDGNASPLVDGQTSYF 242

Query: 245 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 304
           NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG
Sbjct: 243 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 302

Query: 305 TPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARG 364
           TPMRPGPID YLFSLIDED+KSIDPGNFERHWGIFYYDG+PKYQL+LG SNNGSLV A+G
Sbjct: 303 TPMRPGPIDAYLFSLIDEDSKSIDPGNFERHWGIFYYDGQPKYQLSLGNSNNGSLVGAKG 362

Query: 365 VRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSY 424
           VRYLE+KWCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISYAFNSY
Sbjct: 363 VRYLERKWCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISYAFNSY 422

Query: 425 YQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLMLLSGL 484
           YQR+N  ED CKFSGL+ VTN+DPSFG+CRFDIMIE Y G AEGR GC FK+ L L+SG 
Sbjct: 423 YQRSNHAEDACKFSGLSTVTNSDPSFGSCRFDIMIESYMGGAEGRSGCRFKRLLALVSGF 482

Query: 485 LLVL 487
           +LVL
Sbjct: 483 VLVL 486

BLAST of Moc06g14500 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 711.8 bits (1836), Expect = 5.2e-204
Identity = 340/459 (74.07%), Postives = 393/459 (85.62%), Query Frame = 0

Query: 8   MGWFFKAFVGLVSFFSLVCS-VAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDAD 67
           MGW     V L+   +L+C   ++IGANWGTQA+HPLPP+ VV MLR+N IQKVKLFDA+
Sbjct: 1   MGW---GSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAE 60

Query: 68  YGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNE 127
           Y TLRALGK+GIEVMVGIPN+ML +LASS+KAAEKWV+KNVS HIST+NVNIRYVAVGNE
Sbjct: 61  YDTLRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNE 120

Query: 128 PFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTD 187
           PFLSTYNGS+L TTFPAL+N+Q A+IKAGL NQVKVT PLNADVY SSTTFPSGGDFR +
Sbjct: 121 PFLSTYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRAN 180

Query: 188 IHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMF 247
           I DLMI IVKFLS++G PFTVNIYP+ISLY++P+FPV+YAFFDGNA P+ DGGT Y+NMF
Sbjct: 181 IRDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMF 240

Query: 248 DANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPM 307
           DANYDTLV AL+KNGFGN+PII+GEIGWPTDGD NAN  YA++FNQGFM+HI GGKGTP 
Sbjct: 241 DANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPR 300

Query: 308 RPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRY 367
           RPGPID YLFSLIDEDAKS+ PG FERHWGIF +DG PKY LNLGT+N G+L+ A+GVRY
Sbjct: 301 RPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGVRY 360

Query: 368 LEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQR 427
           LE+KWCVMK + RL+D QVAP+VSYAC   DCTSLG GTSC+++D + NISYAFNSYYQ 
Sbjct: 361 LERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQI 420

Query: 428 NNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAA 466
            +Q +  CKF  ++EVT  DPS G CRF IMIEPYYG A
Sbjct: 421 QDQLDTACKFPNISEVTKTDPSTGTCRFPIMIEPYYGGA 456

BLAST of Moc06g14500 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 2.8e-181
Identity = 302/451 (66.96%), Postives = 363/451 (80.49%), Query Frame = 0

Query: 9   GWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYG 68
           G F   FV  + + SL+  V  IG NWG+QA HPLPP TVV +LR+N IQKVKLF+AD  
Sbjct: 5   GVFAVFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSA 64

Query: 69  TLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPF 128
            L+AL +TGI+VMVGIPND+L  LA SV AAE+WVS+NVS H+S+N V+IRYVAVGNEPF
Sbjct: 65  ILKALSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPF 124

Query: 129 LSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIH 188
           L  +NG+F   T PALQN+QSA+IKAGLA QVKVTVPLNADVY S++  PS GDFR +I 
Sbjct: 125 LKAFNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIR 184

Query: 189 DLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDA 248
           DLM+NIVKFLSD+G+PFT+NIYPFISLY+DPNFPVE+AFFDG  +PI D G  Y N+ DA
Sbjct: 185 DLMLNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDA 244

Query: 249 NYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRP 308
           NYDTLVW+LQKNGFGNL IIVGE+GWPTDGD+NAN +YA+R+NQGFM+     KGTPMRP
Sbjct: 245 NYDTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRP 304

Query: 309 GPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRYLE 368
           G +D YLF LIDEDAKSI PGNFERHWGIFY DG+PKYQL+LG+ N   L+ A+ V YL 
Sbjct: 305 GAMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLGSGN--GLIPAKDVHYLA 364

Query: 369 KKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNN 428
           KKWC++  +A L+D Q+ PSVSYAC HADCTSLGYG+SC +++   N+SYAFNSYYQ +N
Sbjct: 365 KKWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSN 424

Query: 429 QGEDDCKFSGLAEVTNNDPSFGNCRFDIMIE 460
           Q +  CKF GL+ V+  DPS G+C+F IMI+
Sbjct: 425 QLDSACKFPGLSIVSTRDPSVGSCKFKIMIK 453

BLAST of Moc06g14500 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 471.9 bits (1213), Expect = 9.0e-132
Identity = 237/434 (54.61%), Postives = 301/434 (69.35%), Query Frame = 0

Query: 28  VAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTLRALGKTGIEVMVGIPND 87
           V  +G NWGT ATH LPP+TVV ML+DN I KVKLFDAD  T+ AL  +G+EVMV IPND
Sbjct: 22  VNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPND 81

Query: 88  MLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPFLSTYNGSFLKTTFPALQNV 147
            L  + +S   A+ WV KNV+ +     VNI +VAVGNEPFL +YNGSF+  TFPAL N+
Sbjct: 82  QL-KVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANI 141

Query: 148 QSALIKAGLANQVKVTVPLNADVYAS--STTFPSGGDFRTDIHDLMINIVKFLSDSGSPF 207
           Q+AL +AGL N VK TVPLNADVY S  S   PS G FR DI   M  IV FL  + +P 
Sbjct: 142 QNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPI 201

Query: 208 TVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDANYDTLVWALQKNGFGNL 267
           T+NIYPF+SLY + +FP+ YAFFDG A PI D G  Y N+FDAN+DTLV +L+  G G++
Sbjct: 202 TINIYPFLSLYGNDDFPLNYAFFDG-AEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDM 261

Query: 268 PIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGPIDTYLFSLIDEDAKS 327
           PIIVGE+GWPT+GD++AN   A RF  G +  +   KGTP+RP  I+ YLF L+DEDAKS
Sbjct: 262 PIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKS 321

Query: 328 IDPGNFERHWGIFYYDGRPKYQLNL-GTSNNGSLVAARGVRYLEKKWCVMKRSARLEDSQ 387
           I PG FERHWGIF +DG+PK+ ++L G   +  L+ A+ V YL  KWC     A+ + ++
Sbjct: 322 IAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAK-DLTK 381

Query: 388 VAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNNQGEDDCKFSGLAEVTN 447
           +A ++ YAC  +DCT+LGYG+SC+++DA  N SYAFN ++Q  NQ E  C F GLA +T 
Sbjct: 382 LAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITT 441

Query: 448 NDPSFGNCRFDIMI 459
            + S G C F I I
Sbjct: 442 QNISQGQCNFPIQI 452

BLAST of Moc06g14500 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.2e-105
Identity = 198/441 (44.90%), Postives = 273/441 (61.90%), Query Frame = 0

Query: 27  SVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTLRALGKTGIEVMVGIPN 86
           +V A+G NWGT+A+HPLPP  VV +L+ N I KVKLFDAD   LRAL  + I V +GI N
Sbjct: 22  TVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGSNIGVTIGIQN 81

Query: 87  DMLYSLASSVKAAEKWVSKNVSVHIS-TNNVNIRYVAVGNEPFLSTYNGSFLKTTFPALQ 146
            ML SL +SVK AE WV  NV+ + +  N V I YVAVG EPFL +Y   +      A  
Sbjct: 82  SMLKSLNASVKVAESWVHDNVTRYFNGGNRVRIEYVAVGEEPFLQSYGNQYKPFVIGAAM 141

Query: 147 NVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIHDLMINIVKFLSDSGSPF 206
           N+Q+AL+KA LAN+VKV VP + D + S +  PS G FR D++  MI ++ FL+   SPF
Sbjct: 142 NIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLNKTMIELLSFLTKHHSPF 201

Query: 207 TVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDANYDTLVWALQKNGFGNL 266
            V I PF+S + + N  ++++ F   A    DG  +Y N FD +YDTLV AL   GF  +
Sbjct: 202 FVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDLSYDTLVSALFTIGFSEV 261

Query: 267 PIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGPIDTYLFSLIDEDAKS 326
            I+V +IGWPTDG  NA  + A+ F +G + H+     +  RP P++TY+ SL+DED ++
Sbjct: 262 DIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASLPRP-PVETYIESLLDEDQRN 321

Query: 327 IDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRYLEKKWCVMKRSARLEDSQV 386
           +  GNFERHWG+F +DG+ KY  +    N+ + V A+ V+YL  KWCV+  +  L  S  
Sbjct: 322 LSAGNFERHWGVFTFDGQAKYNFSF---NHKNQVNAQNVQYLPPKWCVVNNNKDL--SNA 381

Query: 387 APSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNNQGEDDCKFSGLAEVTNN 446
           +     AC  ADCTS+  G SCS +    N+SYAFNS YQ+N+   + C F GL  +T  
Sbjct: 382 SARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESCNFGGLGLITTV 441

Query: 447 DPSFGNCRFDIMIEPYYGAAE 467
           DPS  NCRF I ++  + +++
Sbjct: 442 DPSEDNCRFSIQLDTSHSSSQ 456

BLAST of Moc06g14500 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 3.1e-63
Identity = 156/432 (36.11%), Postives = 236/432 (54.63%), Query Frame = 0

Query: 31  IGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTLRALGKTGIEVMVGIPNDMLY 90
           IG N+G  A +  PP   V +L+   IQKV+L+ AD   ++AL  TG+ +++G  N  + 
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVP 85

Query: 91  SLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPFLSTYNGSFLKTTFPALQNVQSA 150
           SLAS   AA +W++ NV      + + +  + VGNE  +S  + + +    PA+QNVQ A
Sbjct: 86  SLASDPNAATQWINSNVLPFYPASKIML--ITVGNEILMSN-DPNLVNQLLPAMQNVQKA 145

Query: 151 LIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIHDLMINIVKFLSDSGSPFTVNIY 210
           L    L  ++KV+  +N+     S+  PS G F       +  I++FLSD+GSPF +N Y
Sbjct: 146 LEAVSLGGKIKVST-VNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPY 205

Query: 211 PFISLYSDPNFPVEYAF--FDGNASPI-VDGGTSYFNMFDANYDTLVWALQKNGFGNLPI 270
           PF +  SDP  P   AF  F+ NA  +    G  Y NMFDA  D +  AL+  GF  + I
Sbjct: 206 PFFAYQSDPR-PETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEI 265

Query: 271 IVGEIGWPTDGDRN---ANPIYAQRFNQGFMSHILGGKGTPMRPG-PIDTYLFSLIDEDA 330
           +V E GW + GD N   A+   A+ +N   ++H+    GTP+ PG P+DTY+F+L DE+ 
Sbjct: 266 VVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENL 325

Query: 331 KSIDPG-NFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRYLEKKWCVMKRSARLED 390
           K   PG + ER +G+F  D    Y + L  S++ S   +  V      WCV K+ A  E+
Sbjct: 326 K---PGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGKV--TSSGWCVPKKGATNEE 385

Query: 391 SQVAPSVSYACYHA-DCTSLGYGTSCSSVDARL-NISYAFNSYYQRNNQGEDDCKFSGLA 450
            Q   S+ +AC H  DC ++  G +C   +  + + +YA N Y+Q++ +   DC FS  A
Sbjct: 386 LQA--SLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTA 445

Query: 451 EVTNNDPSFGNC 453
            VT+ +PS+ NC
Sbjct: 446 TVTSQNPSYNNC 445

BLAST of Moc06g14500 vs. ExPASy TrEMBL
Match: A0A6J1DSF5 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111023883 PE=3 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 9.9e-291
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MLKNSGKMGWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKV 60
           MLKNSGKMGWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKV
Sbjct: 1   MLKNSGKMGWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKV 60

Query: 61  KLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRY 120
           KLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRY
Sbjct: 61  KLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRY 120

Query: 121 VAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSG 180
           VAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSG
Sbjct: 121 VAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSG 180

Query: 181 GDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGT 240
           GDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGT
Sbjct: 181 GDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGT 240

Query: 241 SYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILG 300
           SYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILG
Sbjct: 241 SYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILG 300

Query: 301 GKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVA 360
           GKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVA
Sbjct: 301 GKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVA 360

Query: 361 ARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAF 420
           ARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAF
Sbjct: 361 ARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAF 420

Query: 421 NSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGRGCCFKKHLMLLS 480
           NSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGRGCCFKKHLMLLS
Sbjct: 421 NSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGRGCCFKKHLMLLS 480

Query: 481 GLLLVLTFL 490
           GLLLVLTFL
Sbjct: 481 GLLLVLTFL 489

BLAST of Moc06g14500 vs. ExPASy TrEMBL
Match: A0A0A0LXZ0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G651130 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 9.4e-249
Identity = 423/485 (87.22%), Postives = 446/485 (91.96%), Query Frame = 0

Query: 5   SGKMG--WFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKL 64
           + KMG  WF +  V LV   S+VCSV AIGANWGTQ++HPLPPETVVGMLRDNQIQKVKL
Sbjct: 4   NSKMGCCWFLRCIVVLVLLLSVVCSVKAIGANWGTQSSHPLPPETVVGMLRDNQIQKVKL 63

Query: 65  FDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVA 124
           FDADYGTLRALGKTGIEVMVGIPNDML + AS+ KAAEKWVSKNVSVHIS NNVNIRYVA
Sbjct: 64  FDADYGTLRALGKTGIEVMVGIPNDMLSTFASNEKAAEKWVSKNVSVHISENNVNIRYVA 123

Query: 125 VGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGD 184
           VGNEPFL+TYNGSFL TTFPAL+NVQ ALIKA L NQVKVT PLNADVYAS+TTFPSGGD
Sbjct: 124 VGNEPFLATYNGSFLSTTFPALRNVQKALIKANLGNQVKVTCPLNADVYASTTTFPSGGD 183

Query: 185 FRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSY 244
           FR+DIHDLM++IVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDG T+Y
Sbjct: 184 FRSDIHDLMLDIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGQTTY 243

Query: 245 FNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGK 304
           FNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGK
Sbjct: 244 FNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGK 303

Query: 305 GTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAAR 364
           GTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQL+LG S NG+LV AR
Sbjct: 304 GTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLSLGNS-NGTLVGAR 363

Query: 365 GVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNS 424
           GV YLE+KWCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISYAFNS
Sbjct: 364 GVHYLERKWCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISYAFNS 423

Query: 425 YYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLMLLSG 484
           YYQRNNQ ED CKFSGL+ VTNNDPSFG+CRFDIMIEPYYG AEGR G CF + LMLL G
Sbjct: 424 YYQRNNQAEDACKFSGLSTVTNNDPSFGSCRFDIMIEPYYGGAEGRSGFCFTRLLMLLFG 483

Query: 485 LLLVL 487
            +LV+
Sbjct: 484 FVLVM 487

BLAST of Moc06g14500 vs. ExPASy TrEMBL
Match: A0A1S3B8H9 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487193 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.8e-245
Identity = 420/489 (85.89%), Postives = 447/489 (91.41%), Query Frame = 0

Query: 1   MLKNSGKMG--WFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQ 60
           ML NS KMG   F ++ V LV   S+VCSV AIGANWGTQ++HPLPPETVVGMLRDNQIQ
Sbjct: 1   MLFNS-KMGCCCFLRSTVVLVLLLSVVCSVKAIGANWGTQSSHPLPPETVVGMLRDNQIQ 60

Query: 61  KVKLFDADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNI 120
           KVKLFDADYGTLRALGKTGIEVMVGIPNDML + A+S KAAEKWVSKNVSVHIS NNVNI
Sbjct: 61  KVKLFDADYGTLRALGKTGIEVMVGIPNDMLSTFANSEKAAEKWVSKNVSVHISENNVNI 120

Query: 121 RYVAVGNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFP 180
           RYVAVGNEPFL+TYNGSFL TTFPAL+NVQ ALIKA L NQVKVT PLNADVYAS+T+ P
Sbjct: 121 RYVAVGNEPFLATYNGSFLSTTFPALRNVQRALIKANLGNQVKVTCPLNADVYASTTSLP 180

Query: 181 SGGDFRTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDG 240
           SGGDFR+DIHDLM+ IVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDG
Sbjct: 181 SGGDFRSDIHDLMLAIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDG 240

Query: 241 GTSYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHI 300
            T+YFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHI
Sbjct: 241 QTTYFNMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHI 300

Query: 301 LGGKGTPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSL 360
           LGGKGTPMRPGPID YLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQL+LG SNN +L
Sbjct: 301 LGGKGTPMRPGPIDAYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLSLGNSNNVTL 360

Query: 361 VAARGVRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISY 420
           V A+GVRYLE++WCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISY
Sbjct: 361 VGAKGVRYLERRWCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISY 420

Query: 421 AFNSYYQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLM 480
           AFNSYYQR+NQ ED CKFSGL+ VTNNDPSFG+CRFDIMIEPYYG AEGR G CF + LM
Sbjct: 421 AFNSYYQRSNQAEDACKFSGLSTVTNNDPSFGSCRFDIMIEPYYGGAEGRSGFCFTRLLM 480

Query: 481 LLSGLLLVL 487
           L+ G +LV+
Sbjct: 481 LVFGFVLVM 488

BLAST of Moc06g14500 vs. ExPASy TrEMBL
Match: A0A6J1EDY5 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111433399 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.4e-244
Identity = 415/484 (85.74%), Postives = 443/484 (91.53%), Query Frame = 0

Query: 5   SGKMG-WFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLF 64
           + KMG  F    VGL+S  S+VCSV AIGANWGTQ++HPLPPETVVGMLRDNQIQKVKLF
Sbjct: 3   NSKMGCCFLHVSVGLLSLISMVCSVVAIGANWGTQSSHPLPPETVVGMLRDNQIQKVKLF 62

Query: 65  DADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAV 124
           DADYGTLRALGKTGIEVMVGIPNDML +LA+S KAAEKWVSKNVSVHIS NNVNIRYVAV
Sbjct: 63  DADYGTLRALGKTGIEVMVGIPNDMLSTLANSEKAAEKWVSKNVSVHISDNNVNIRYVAV 122

Query: 125 GNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDF 184
           GNEPFLSTYNGSFL TTFPALQN+Q AL+KA L NQVKVTVPLNADVY SSTTFPSGG+F
Sbjct: 123 GNEPFLSTYNGSFLSTTFPALQNIQRALLKANLGNQVKVTVPLNADVYESSTTFPSGGNF 182

Query: 185 RTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYF 244
           R DIHDLM+ IVKFLSD+GSPFTVNIYPFISLYS PNFPVEYAFFDGNASP+VDG TSYF
Sbjct: 183 RADIHDLMLAIVKFLSDTGSPFTVNIYPFISLYSTPNFPVEYAFFDGNASPLVDGQTSYF 242

Query: 245 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 304
           NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG
Sbjct: 243 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 302

Query: 305 TPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARG 364
           TPMRPGPID YLFSLIDED+KSIDPGNFERHWG+FYYDG+PKYQL+LG SNNGSLV A+G
Sbjct: 303 TPMRPGPIDAYLFSLIDEDSKSIDPGNFERHWGVFYYDGQPKYQLSLGNSNNGSLVGAKG 362

Query: 365 VRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSY 424
           VRYL++KWCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISYAFNSY
Sbjct: 363 VRYLKRKWCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISYAFNSY 422

Query: 425 YQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLMLLSGL 484
           YQR+NQ ED CKFSGL+ VTN+DPSFG+CRFDIMIE   G AEGR GC FK+ L L+SG 
Sbjct: 423 YQRSNQAEDACKFSGLSTVTNSDPSFGSCRFDIMIEANMGGAEGRSGCRFKRLLALVSGF 482

Query: 485 LLVL 487
           +LVL
Sbjct: 483 VLVL 486

BLAST of Moc06g14500 vs. ExPASy TrEMBL
Match: A0A6J1I8N3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472428 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.8e-244
Identity = 415/484 (85.74%), Postives = 443/484 (91.53%), Query Frame = 0

Query: 5   SGKMG-WFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLF 64
           + KMG  F    VGL+S  S+VCSV AIGANWGTQ++HPLPPETVVGMLRDNQIQKVKLF
Sbjct: 3   NSKMGCCFLHGSVGLLSLISMVCSVVAIGANWGTQSSHPLPPETVVGMLRDNQIQKVKLF 62

Query: 65  DADYGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAV 124
           DADYGTLRALGKTGIEVMVGIPNDML +LA+S KAAEKWVSKNVSVHIS NNVNIRYVAV
Sbjct: 63  DADYGTLRALGKTGIEVMVGIPNDMLSTLANSEKAAEKWVSKNVSVHISDNNVNIRYVAV 122

Query: 125 GNEPFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDF 184
           GNEPFLSTYNGSFL TTFPALQN+Q AL+KA L NQVKVTVPLNADVY SSTTFPSGG+F
Sbjct: 123 GNEPFLSTYNGSFLSTTFPALQNIQRALLKANLGNQVKVTVPLNADVYESSTTFPSGGNF 182

Query: 185 RTDIHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYF 244
           R DIHDLM+ IVKFLSD+GSPFTVNIYPFISLYS PNFPVEYAFFDGNASP+VDG TSYF
Sbjct: 183 RADIHDLMLAIVKFLSDTGSPFTVNIYPFISLYSTPNFPVEYAFFDGNASPLVDGQTSYF 242

Query: 245 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 304
           NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG
Sbjct: 243 NMFDANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKG 302

Query: 305 TPMRPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARG 364
           TPMRPGPID YLFSLIDED+KSIDPGNFERHWG+FYYDG+PKYQL+LG SNNG LV A+G
Sbjct: 303 TPMRPGPIDAYLFSLIDEDSKSIDPGNFERHWGVFYYDGQPKYQLSLGNSNNGPLVGAKG 362

Query: 365 VRYLEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSY 424
           VRYLE+KWCVMK SA LEDSQVAPSVSYACYHADCTSLGYGTSCS +DAR NISYAFNSY
Sbjct: 363 VRYLERKWCVMKPSAHLEDSQVAPSVSYACYHADCTSLGYGTSCSGLDARSNISYAFNSY 422

Query: 425 YQRNNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAAEGR-GCCFKKHLMLLSGL 484
           YQR+NQ ED CKFSGL+ VTN+DPSFG+CRFDIMIE   G AEGR GCCFK+ L L+SG 
Sbjct: 423 YQRSNQAEDACKFSGLSTVTNSDPSFGSCRFDIMIED-MGGAEGRSGCCFKRLLALVSGY 482

Query: 485 LLVL 487
           +L+L
Sbjct: 483 VLIL 485

BLAST of Moc06g14500 vs. TAIR 10
Match: AT5G58090.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 711.8 bits (1836), Expect = 3.7e-205
Identity = 340/459 (74.07%), Postives = 393/459 (85.62%), Query Frame = 0

Query: 8   MGWFFKAFVGLVSFFSLVCS-VAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDAD 67
           MGW     V L+   +L+C   ++IGANWGTQA+HPLPP+ VV MLR+N IQKVKLFDA+
Sbjct: 1   MGW---GSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAE 60

Query: 68  YGTLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNE 127
           Y TLRALGK+GIEVMVGIPN+ML +LASS+KAAEKWV+KNVS HIST+NVNIRYVAVGNE
Sbjct: 61  YDTLRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNE 120

Query: 128 PFLSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTD 187
           PFLSTYNGS+L TTFPAL+N+Q A+IKAGL NQVKVT PLNADVY SSTTFPSGGDFR +
Sbjct: 121 PFLSTYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRAN 180

Query: 188 IHDLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMF 247
           I DLMI IVKFLS++G PFTVNIYP+ISLY++P+FPV+YAFFDGNA P+ DGGT Y+NMF
Sbjct: 181 IRDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMF 240

Query: 248 DANYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPM 307
           DANYDTLV AL+KNGFGN+PII+GEIGWPTDGD NAN  YA++FNQGFM+HI GGKGTP 
Sbjct: 241 DANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPR 300

Query: 308 RPGPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRY 367
           RPGPID YLFSLIDEDAKS+ PG FERHWGIF +DG PKY LNLGT+N G+L+ A+GVRY
Sbjct: 301 RPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGVRY 360

Query: 368 LEKKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQR 427
           LE+KWCVMK + RL+D QVAP+VSYAC   DCTSLG GTSC+++D + NISYAFNSYYQ 
Sbjct: 361 LERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQI 420

Query: 428 NNQGEDDCKFSGLAEVTNNDPSFGNCRFDIMIEPYYGAA 466
            +Q +  CKF  ++EVT  DPS G CRF IMIEPYYG A
Sbjct: 421 QDQLDTACKFPNISEVTKTDPSTGTCRFPIMIEPYYGGA 456

BLAST of Moc06g14500 vs. TAIR 10
Match: AT4G31140.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 636.3 bits (1640), Expect = 2.0e-182
Identity = 302/451 (66.96%), Postives = 363/451 (80.49%), Query Frame = 0

Query: 9   GWFFKAFVGLVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYG 68
           G F   FV  + + SL+  V  IG NWG+QA HPLPP TVV +LR+N IQKVKLF+AD  
Sbjct: 5   GVFAVFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSA 64

Query: 69  TLRALGKTGIEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPF 128
            L+AL +TGI+VMVGIPND+L  LA SV AAE+WVS+NVS H+S+N V+IRYVAVGNEPF
Sbjct: 65  ILKALSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPF 124

Query: 129 LSTYNGSFLKTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIH 188
           L  +NG+F   T PALQN+QSA+IKAGLA QVKVTVPLNADVY S++  PS GDFR +I 
Sbjct: 125 LKAFNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIR 184

Query: 189 DLMINIVKFLSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDA 248
           DLM+NIVKFLSD+G+PFT+NIYPFISLY+DPNFPVE+AFFDG  +PI D G  Y N+ DA
Sbjct: 185 DLMLNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDA 244

Query: 249 NYDTLVWALQKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRP 308
           NYDTLVW+LQKNGFGNL IIVGE+GWPTDGD+NAN +YA+R+NQGFM+     KGTPMRP
Sbjct: 245 NYDTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRP 304

Query: 309 GPIDTYLFSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRYLE 368
           G +D YLF LIDEDAKSI PGNFERHWGIFY DG+PKYQL+LG+ N   L+ A+ V YL 
Sbjct: 305 GAMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLGSGN--GLIPAKDVHYLA 364

Query: 369 KKWCVMKRSARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNN 428
           KKWC++  +A L+D Q+ PSVSYAC HADCTSLGYG+SC +++   N+SYAFNSYYQ +N
Sbjct: 365 KKWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSN 424

Query: 429 QGEDDCKFSGLAEVTNNDPSFGNCRFDIMIE 460
           Q +  CKF GL+ V+  DPS G+C+F IMI+
Sbjct: 425 QLDSACKFPGLSIVSTRDPSVGSCKFKIMIK 453

BLAST of Moc06g14500 vs. TAIR 10
Match: AT5G20870.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 538.5 bits (1386), Expect = 5.6e-153
Identity = 268/445 (60.22%), Postives = 328/445 (73.71%), Query Frame = 0

Query: 23  SLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTLRALGKTGIEVMV 82
           +++ +V  +  NWGTQA+HPLPP  VV +LRDN   KVKLF+AD G LRALGK+GI+VMV
Sbjct: 22  NILKNVEGLACNWGTQASHPLPPNIVVKLLRDNGFNKVKLFEADPGALRALGKSGIQVMV 81

Query: 83  GIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPFLSTYNGSFLKTTFP 142
           GIPND+L ++AS+V  AE WV +NVS +IS    +IRYVAVGNEPFL TYN  F+++T+P
Sbjct: 82  GIPNDLLATMASTVTNAELWVQQNVSQYISRYGTDIRYVAVGNEPFLKTYNNRFVRSTYP 141

Query: 143 ALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIHDLMINIVKFLSDSG 202
           ALQNVQ+AL+KAGL  QVKVTVPLNADVY SS   PS GDFR+DI  LMI+IV+FL+DS 
Sbjct: 142 ALQNVQAALVKAGLGRQVKVTVPLNADVYESSDGLPSSGDFRSDIKTLMISIVRFLADSV 201

Query: 203 SPFTVNIYPFISLYSDPNFPVEYAFF-----DGNASPIVDGGTSYFNMFDANYDTLVWAL 262
           SP T NIYPF+SL +DPNFP EYAFF      G A P+VDG  SY N+FDAN+DTLV AL
Sbjct: 202 SPITFNIYPFLSLNADPNFPREYAFFPNGGGGGGAKPVVDGSISYTNVFDANFDTLVSAL 261

Query: 263 QKNGF--GNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGPIDTYL 322
           +KNGF    + IIVGE+GWPTDGD+NANP  AQRFNQG ++ IL G+GTP R    + Y+
Sbjct: 262 EKNGFDANKIEIIVGEVGWPTDGDQNANPAMAQRFNQGLLNRILQGQGTPRRRMAPEVYI 321

Query: 323 FSLIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNLGTSNNGSLVAARGVRYLEKKWCVMK 382
           FSL+DEDAKSIDPG FERHWGIF YDG  KY L+LG  N   LV  +GVRY  ++WCV+ 
Sbjct: 322 FSLVDEDAKSIDPGKFERHWGIFSYDGAVKYPLSLG--NGRPLVPTKGVRYQAREWCVLS 381

Query: 383 RSARLEDSQV-APSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNNQGEDDC 442
             A    +     S +YAC +ADCTSLG G+SC+++D   N SYAFN Y+Q+ +     C
Sbjct: 382 TQAAGNGAATWQASATYACQNADCTSLGPGSSCAALDPTANASYAFNMYFQKMDHRRGSC 441

Query: 443 KFSGLAEVTNNDPSFGNCRFDIMIE 460
            F+ L  VT  DPS G+CRF I I+
Sbjct: 442 DFNNLGVVTKIDPSSGSCRFPIEID 464

BLAST of Moc06g14500 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 478.4 bits (1230), Expect = 6.9e-135
Identity = 238/437 (54.46%), Postives = 304/437 (69.57%), Query Frame = 0

Query: 25  VCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTLRALGKTGIEVMVGI 84
           +  V  +G NWGT ATH LPP+ VV ML+DN I KVKLFDAD  T+ AL  +G+EVMV I
Sbjct: 15  IVMVDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAI 74

Query: 85  PNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPFLSTYNGSFLKTTFPAL 144
           PND L  +  S   A+ WV KNV+ +     VNI +VAVGNEPFL +YNGSF+  TFPAL
Sbjct: 75  PNDQL-KVMGSYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPAL 134

Query: 145 QNVQSALIKAGLANQVKVTVPLNADVY--ASSTTFPSGGDFRTDIHDLMINIVKFLSDSG 204
           QN+Q+AL +AGL + VK TVPLNADVY   SS   PS G FR DI   M  IV FL ++ 
Sbjct: 135 QNIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNS 194

Query: 205 SPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDANYDTLVWALQKNGF 264
           +P T+NIYPF+SLY + +FP+ YAFFDG A P+ D G +Y N+FDAN+DTLV AL+  G 
Sbjct: 195 APITINIYPFLSLYGNDDFPLNYAFFDG-AKPVDDNGIAYTNVFDANFDTLVSALKAVGH 254

Query: 265 GNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGPIDTYLFSLIDED 324
           G++PIIVGE+GWPT+GD++AN   A RF  G +  +   +GTP+RP  I+ YLF L+DED
Sbjct: 255 GDMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDED 314

Query: 325 AKSIDPGNFERHWGIFYYDGRPKYQLNL-GTSNNGSLVAARGVRYLEKKWCVMKRSARLE 384
           AKSI PG FERHWGIF +DG+PK+ ++L G   N  L+ A  V Y  KKWC+    A+ +
Sbjct: 315 AKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAK-D 374

Query: 385 DSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNNQGEDDCKFSGLAE 444
            +++A ++ YAC  +DCT+LGYG+SC+++DA  N SYAFN Y+Q  NQ ED C F GLA 
Sbjct: 375 LTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLAT 434

Query: 445 VTNNDPSFGNCRFDIMI 459
           +T  + S G C F I I
Sbjct: 435 ITTKNISQGQCNFPIQI 448

BLAST of Moc06g14500 vs. TAIR 10
Match: AT5G64790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 473.0 bits (1216), Expect = 2.9e-133
Identity = 230/442 (52.04%), Postives = 301/442 (68.10%), Query Frame = 0

Query: 18  LVSFFSLVCSVAAIGANWGTQATHPLPPETVVGMLRDNQIQKVKLFDADYGTLRALGKTG 77
           +++  +L   V   G NWG  A+HPL P  VV ML+DN+I KVKLFDAD  T+ AL  TG
Sbjct: 15  IMTVLTLASLVGGFGVNWGNIASHPLNPNIVVQMLKDNKINKVKLFDADSWTMNALAGTG 74

Query: 78  IEVMVGIPNDMLYSLASSVKAAEKWVSKNVSVHISTNNVNIRYVAVGNEPFLSTYNGSFL 137
           +EVMVGIPN++L SLA     A+ WV +NV+ +I    V+I+YVAVGNEPFLS YNGSFL
Sbjct: 75  MEVMVGIPNNLLESLADDYDNAKDWVKENVTQYIRKGGVDIKYVAVGNEPFLSAYNGSFL 134

Query: 138 KTTFPALQNVQSALIKAGLANQVKVTVPLNADVYASSTTFPSGGDFRTDIHDLMINIVKF 197
           KTTFPAL+N+  AL +AG  N +K T+P NA+VY S+   PS GDFR D+   M++IV F
Sbjct: 135 KTTFPALKNIHKALKEAGHTNIMKATIPQNAEVYQSANDKPSEGDFRKDVKQTMLDIVNF 194

Query: 198 LSDSGSPFTVNIYPFISLYSDPNFPVEYAFFDGNASPIVDGGTSYFNMFDANYDTLVWAL 257
             ++  PFTVNIYPF+SLY + +FPVE+AF DG+   + D G +Y N+FDANYDTLV+AL
Sbjct: 195 FHENDLPFTVNIYPFLSLYLNEHFPVEFAFLDGDGQTMTDKGKNYDNVFDANYDTLVYAL 254

Query: 258 QKNGFGNLPIIVGEIGWPTDGDRNANPIYAQRFNQGFMSHILGGKGTPMRPGPIDTYLFS 317
           +K G  ++ IIVGE+GWPTDG + A+P  A++F  G M  +    GTP RP  ++ YLF 
Sbjct: 255 KKAGIHDMKIIVGEVGWPTDGHKYASPKLAEKFYAGLMKRLAKDGGTPTRPERLEVYLFG 314

Query: 318 LIDEDAKSIDPGNFERHWGIFYYDGRPKYQLNL-GTSNNGSLVAARGVRYLEKKWCVMKR 377
            +DED KSI PG FERHWGIF YDG PK+ L+  G       VAA+GV+YLEK+WCV+ +
Sbjct: 315 FLDEDMKSILPGPFERHWGIFRYDGTPKFMLDFTGQGRQMVPVAAKGVQYLEKQWCVVNK 374

Query: 378 SARLEDSQVAPSVSYACYHADCTSLGYGTSCSSVDARLNISYAFNSYYQRNNQGEDDCKF 437
              +   +V P + YACYH DCT++  G++CS +    NISYAFN Y+Q  +Q    C F
Sbjct: 375 DT-VNLDEVGPDLDYACYHGDCTAMEAGSTCSKLTKVQNISYAFNMYFQIQDQDVRACDF 434

Query: 438 SGLAEVTNNDPSFGNCRFDIMI 459
            G A +T  + S G+C F + I
Sbjct: 435 KGAAMITKVNASVGSCLFPVQI 455

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157073.12.0e-290100.00glucan endo-1,3-beta-glucosidase 6 [Momordica charantia][more]
XP_038879688.12.4e-25188.68glucan endo-1,3-beta-glucosidase 6 [Benincasa hispida][more]
XP_011660196.11.9e-24887.22glucan endo-1,3-beta-glucosidase 6 [Cucumis sativus] >KGN66628.1 hypothetical pr... [more]
KAG7022177.11.4e-24686.16Glucan endo-1,3-beta-glucosidase 6 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023520642.13.4e-24586.16glucan endo-1,3-beta-glucosidase 6-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q93Z085.2e-20474.07Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9M0882.8e-18166.96Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q6NKW99.0e-13254.61Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9FGH43.2e-10544.90Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q9M0693.1e-6336.11Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DSF59.9e-291100.00(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111023883 ... [more]
A0A0A0LXZ09.4e-24987.22(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G651130 PE=3... [more]
A0A1S3B8H92.8e-24585.89(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487193 PE=3 SV... [more]
A0A6J1EDY51.4e-24485.74(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111433399 P... [more]
A0A6J1I8N31.8e-24485.74(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472428 PE=... [more]
Match NameE-valueIdentityDescription
AT5G58090.13.7e-20574.07O-Glycosyl hydrolases family 17 protein [more]
AT4G31140.12.0e-18266.96O-Glycosyl hydrolases family 17 protein [more]
AT5G20870.15.6e-15360.22O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.16.9e-13554.46O-Glycosyl hydrolases family 17 protein [more]
AT5G64790.12.9e-13352.04O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 370..454
e-value: 4.8E-34
score: 129.1
IPR012946X8 domainPFAMPF07983X8coord: 370..440
e-value: 2.5E-16
score: 60.1
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 31..349
e-value: 8.6E-66
score: 222.7
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 265..278
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..357
e-value: 1.2E-115
score: 387.8
NoneNo IPR availableGENE3D1.20.58.1040coord: 366..456
e-value: 6.1E-8
score: 34.9
NoneNo IPR availablePANTHERPTHR32227:SF20GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 6coord: 17..468
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 17..468
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 31..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g14500.1Moc06g14500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds