Moc06g14380 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g14380
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr6: 11282291 .. 11282736 (+)
RNA-Seq ExpressionMoc06g14380
SyntenyMoc06g14380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGAATTGTTGCAGGCCTTGCAAGACAGGTATGAGAAACCTTCTGCTAACACTAAAATATTTCTATGGACGAAGTATTTCAATATCCACATGGAGGAGGGAACCATGATGAATTCTCATATTAATGAGCACACCGACATATTAAACAAACTAGAAAGGATGGGCATCAAGATTGATGAGGATGTGAAAGCTATGAGGTTGTTGACATCTATGCCTGACAGTTAGGAGACGATGAGGACCGTGGTGTCGAATTCGTTAGGGGACAATAGCTTAAAATTTTTAGCTATTTGTGATGTTGCCTTGTCTGAGGAAGCCCGGAGGAAATTAGGGAAAATGTCTGCATCTACTTCAAGGGCAGACAACATGGTGAATCAGCTTTGGTGGCTCACGATAAAGGGAAGGCCAAGATGAATTACACGGGGAAACAACAGTATAGAAATTGA

mRNA sequence

ATGAAAGAATTGTTGCAGGCCTTGCAAGACAGGTATGAGAAACCTTCTGCTAACACTAAAATATTTCTATGGACGAAGTATTTCAATATCCACATGGAGGAGGGAACCATGATGAATTCTCATATTAATGAGCACACCGACATATTAAACAAACTAGAAAGGATGGGCATCAAGATTGATGAGGATGTGAAAGCTATGAGGTTGTTGACATCTATGCCTGACAGAAGCCCGGAGGAAATTAGGGAAAATGTCTGCATCTACTTCAAGGGCAGACAACATGGTGAATCAGCTTTGGTGGCTCACGATAAAGGGAAGGCCAAGATGAATTACACGGGGAAACAACAGTATAGAAATTGA

Coding sequence (CDS)

ATGAAAGAATTGTTGCAGGCCTTGCAAGACAGGTATGAGAAACCTTCTGCTAACACTAAAATATTTCTATGGACGAAGTATTTCAATATCCACATGGAGGAGGGAACCATGATGAATTCTCATATTAATGAGCACACCGACATATTAAACAAACTAGAAAGGATGGGCATCAAGATTGATGAGGATGTGAAAGCTATGAGGTTGTTGACATCTATGCCTGACAGAAGCCCGGAGGAAATTAGGGAAAATGTCTGCATCTACTTCAAGGGCAGACAACATGGTGAATCAGCTTTGGTGGCTCACGATAAAGGGAAGGCCAAGATGAATTACACGGGGAAACAACAGTATAGAAATTGA

Protein sequence

MKELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDEDVKAMRLLTSMPDRSPEEIRENVCIYFKGRQHGESALVAHDKGKAKMNYTGKQQYRN
Homology
BLAST of Moc06g14380 vs. NCBI nr
Match: KAF5762583.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 76.6 bits (187), Expect = 1.6e-10
Identity = 34/73 (46.58%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  K FN+ M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 82  RDMLEALSNMYEKPSAGNRVFLMRKLFNMRMNEGSSVADHINEVNSILSRLATVGMKLDD 141

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 142 DTQAVVLLSSLPD 154

BLAST of Moc06g14380 vs. NCBI nr
Match: KAF5803956.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 75.9 bits (185), Expect = 2.7e-10
Identity = 34/73 (46.58%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  + FNI M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 82  RDMLEALSNMYEKPSAGNRVFLMRELFNIRMNEGSSVVDHINEVNSILSRLATVGMKLDD 141

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 142 DTQAVVLLSSLPD 154

BLAST of Moc06g14380 vs. NCBI nr
Match: KAF5775658.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 75.5 bits (184), Expect = 3.5e-10
Identity = 33/73 (45.21%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  + FN+ M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 82  RDMLEALSNMYEKPSAGNRVFLMRELFNMRMNEGSSVADHINEVNSILSRLSTVGMKLDD 141

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 142 DTQAVVLLSSLPD 154

BLAST of Moc06g14380 vs. NCBI nr
Match: KAF5772664.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 75.5 bits (184), Expect = 3.5e-10
Identity = 34/73 (46.58%), Postives = 53/73 (72.60%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L AL + YEKPSA  ++FL  + FN+ M EG+ +  HINE   IL++L  MG+K+D+
Sbjct: 82  RDMLAALSNMYEKPSAGNRVFLMRELFNMRMSEGSSVADHINEVNSILSRLATMGMKLDD 141

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 142 DTQAVVLLSSLPD 154

BLAST of Moc06g14380 vs. NCBI nr
Match: KAF5762683.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 75.1 bits (183), Expect = 4.5e-10
Identity = 33/73 (45.21%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  + FN+ M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 213 RDMLEALSNMYEKPSAGNRVFLMRELFNMRMNEGSSVADHINEVNSILSRLATVGMKLDD 272

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 273 DTQAVVLLSSLPD 285

BLAST of Moc06g14380 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 7.0e-06
Identity = 23/66 (34.85%), Postives = 40/66 (60.61%), Query Frame = 0

Query: 8   LQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDEDVKAMR 67
           L+  Y   +   K++L  + + +HM EGT   SH+N    ++ +L  +G+KI+E+ KA+ 
Sbjct: 88  LESLYMSKTLTNKLYLKKQLYALHMSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAIL 147

Query: 68  LLTSMP 74
           LL S+P
Sbjct: 148 LLNSLP 153

BLAST of Moc06g14380 vs. ExPASy TrEMBL
Match: A0A0D3CPI7 (CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 5.8e-11
Identity = 43/115 (37.39%), Postives = 70/115 (60.87%), Query Frame = 0

Query: 4   LLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDEDV 63
           L++ L D YEKPSAN K+FL  K F++ MEEG ++++H+NE   I+N+L  + I+ D++V
Sbjct: 60  LMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGLVSAHVNEFNTIVNQLSSVEIEFDDEV 119

Query: 64  KAMRLLTSMPDRSPEEIRENVCIYFKGRQHGESALVAHDKGKAKMNYTGKQQYRN 119
           +A+ LL S+P+ S E +R               A V++  G  K+N+   + +RN
Sbjct: 120 RALILLASLPN-SWEPMR---------------AAVSNFVGTQKLNFNDVRDWRN 158

BLAST of Moc06g14380 vs. ExPASy TrEMBL
Match: A0A251S4W8 (Putative zinc finger, CCHC-type, Tubby C-terminal-like domain protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr15g0463441 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-10
Identity = 33/73 (45.21%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  + FN+ M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 344 RDMLEALSNMYEKPSAGNRVFLMRELFNMRMNEGSSVADHINEVNSILSRLATVGMKLDD 403

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 404 DTQAVVLLSSLPD 416

BLAST of Moc06g14380 vs. ExPASy TrEMBL
Match: A0A251UE98 (Putative bulb-type lectin domain-containing protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr07g0204141 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-10
Identity = 33/73 (45.21%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  + FN+ M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 569 RDMLEALSNMYEKPSAGNRVFLMRELFNMRMNEGSSVADHINEVNSILSRLATVGMKLDD 628

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 629 DTQAVVLLSSLPD 641

BLAST of Moc06g14380 vs. ExPASy TrEMBL
Match: A0A251SVN6 (Putative AMP-dependent synthetase/ligase, Zinc finger, CCHC-type OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr13g0415171 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-10
Identity = 33/73 (45.21%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  + FN+ M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 663 RDMLEALSNMYEKPSAGNRVFLMRELFNMRMNEGSSVADHINEVNSILSRLATVGMKLDD 722

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 723 DTQAVVLLSSLPD 735

BLAST of Moc06g14380 vs. ExPASy TrEMBL
Match: A0A251T759 (Putative zinc finger, CCHC-type OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr11g0321451 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-10
Identity = 33/73 (45.21%), Postives = 54/73 (73.97%), Query Frame = 0

Query: 2   KELLQALQDRYEKPSANTKIFLWTKYFNIHMEEGTMMNSHINEHTDILNKLERMGIKIDE 61
           +++L+AL + YEKPSA  ++FL  + FN+ M EG+ +  HINE   IL++L  +G+K+D+
Sbjct: 61  RDMLEALSNMYEKPSAGNRVFLMRELFNMRMNEGSSVADHINEVNSILSRLATVGMKLDD 120

Query: 62  DVKAMRLLTSMPD 75
           D +A+ LL+S+PD
Sbjct: 121 DTQAVVLLSSLPD 133

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAF5762583.11.6e-1046.58putative RNA-directed DNA polymerase [Helianthus annuus][more]
KAF5803956.12.7e-1046.58putative RNA-directed DNA polymerase [Helianthus annuus][more]
KAF5775658.13.5e-1045.21putative RNA-directed DNA polymerase [Helianthus annuus][more]
KAF5772664.13.5e-1046.58putative RNA-directed DNA polymerase [Helianthus annuus][more]
KAF5762683.14.5e-1045.21putative RNA-directed DNA polymerase [Helianthus annuus][more]
Match NameE-valueIdentityDescription
P109787.0e-0634.85Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
A0A0D3CPI75.8e-1137.39CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376... [more]
A0A251S4W82.2e-1045.21Putative zinc finger, CCHC-type, Tubby C-terminal-like domain protein OS=Heliant... [more]
A0A251UE982.2e-1045.21Putative bulb-type lectin domain-containing protein OS=Helianthus annuus OX=4232... [more]
A0A251SVN62.2e-1045.21Putative AMP-dependent synthetase/ligase, Zinc finger, CCHC-type OS=Helianthus a... [more]
A0A251T7592.2e-1045.21Putative zinc finger, CCHC-type OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr11g03... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 2..74
e-value: 5.0E-17
score: 61.9
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 3..75
NoneNo IPR availablePANTHERPTHR34676:SF1ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATEDcoord: 3..75

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g14380.1Moc06g14380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0043167 ion binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0016740 transferase activity