Moc06g14320 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g14320
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionbeta-amyrin 28-oxidase
Locationchr6: 11173582 .. 11177070 (+)
RNA-Seq ExpressionMoc06g14320
SyntenyMoc06g14320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCATTTTCTGGTCTCTCTGTTGATATTTTTCGTGTCCGTTGTCTGCGTGGGCCTCTTCATCCTGTTCTACAAGCACAAATCCCCGTTCTCGTATCCAAACATTCCTCCCGGCGGCATCGGATTTCCGATCATCGGCGAGAGCCTGGAGTTCTTCTCCACCGGATGGAAAGGCGAGCCGGAGAAGTTCATCTTCGACCGCATCAACAAGTACAAGTCCCACATCTTCAAGACCTCCATCGTCGGCGCTCCGGCCGCCGTCTTCTGCGGCCCCACCTGCAACAAGTTCCTCTTCTCCAACGAGAACAAGCTCGTCACCGCCTGGTGGCCCGACTCCGTCAACAAAATCTTCCCCTCTTCCACCCAGACCAGCTCCATGGAGGAGGCCAAGAAACTCAAGAAGCTCCTCCCCCAGTTCCTTAAGCCCGAGGCTCTCCACCGTTACATCGGAATCATGGATGCCCTCGCCCACCGCCACTTCGCTTCCTCCTGGGAGAACAAGGACCAAGTCCTCGTCTTCCCCCTCGCCAAGAGGTAACCACTTTGACACCATGTTTAGGATCAATAAATCACTTCTCAATAATTCAAAAGCTTAAGCGGATAGGTTCGACAAGTTTAATTCTCCATTTACAGCTTCACCTTCTCGTTGGCGTGCCGGCTGTTCGTGAGCGTGGAGGACGAAGCGCACGTGGAGAGGCTATCGGGGCCGTTTCAGCACATTGCGGCGGGGATCATATCGATGCCGATAGATTTTCCAGGAACGCCGTTCAATCGAGGAATCAAGGCGTCGAACTTCATCCGGAAGGAACTCCTGTCGATCGTGAAGCAGAGGAAAGTGGATCTGGCGGAAGGGAAGGCGTCGCCCACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGAGCGTACATGAACGAATTGGACATCGCCGATAAGATTCTCGGCCTCTTGATCGGCGGCCACGACACCGCCAGCGCCGCCTGCACCTTCATCGTCAAGTTCCTCGCTGAGCTTCCCCACATCTACGACGGCGTTTACAAAGGTCAGCCACCTTTCATTTCTTTTTCACACATAAACCCCATTTAATTCCCTTCTAATATTAAACAAAAACTTCAAAACTTTCCAAAAAAATTAAATTGGTAATCTATCTTAATAGATTCAAACTCACTAAATTTTTTGTCAAAGTAAACATAGTTGGTTAAAAAACAAAAAATTGACTACCTTTTAGTGGTAGATTAATAAGGGGTAGTATATTCGAACTTATCTATTTGTTAAAAATTAAAACCAAAGGTAAGAATCTCTAATTTTTTAAAATATACATTCTAAAACTTTTAAGAATCGGAATAAAACAAGTTTAAGAATAGAGGGGTAAGATAACGTTTTAGTATTTTTTCTCTTTTCCCCAAATATTTCATTTTTCCTTTAATGTTTCTCTACCCCATTTAATTTACTTTTTTCTCTTTCACATGAATTTCTACCATAATTTTAAAGTTTCTAGTAAGAAAAGGTGAAAATGTTGACATTTTACCAAAGAGACAAAAGAGGAATAGTGGCTACTGTGGAAGGACAACCAATCGAAACAATAGGGAAAATTAATTTTGTGTAAATTTTGAAATGATAAAATTTAGGTGCTCGCTGATAAGTAGGTATCTTTCTACTTAATATTATTAAAAAATTAACAACTCTTTATTTTTTTTTCTTTCAATCTCTTTATACATTCATTTATAATGCCAGTAGATCTAAGAAGTTAAAAGAGGGAATTTTGAAAGAGATGGGGAAGAGAGAGGAGAGAGAATAAAAACCAACCATTGGTCCTTATGAAAATGATAAATGGTAATACACGATTTAAGGTAAATCGCGATATGTCTATACGATTTATGTAAATTGTGTTGTTTTCACCTTTTAATTACCTATTTTTGGAATTAGTTTTAAAACTACCTTATTTTTCTTAAAAAATATATTATTTATGAATTTTTTTTTCTAAAAAGACAAAGTTGAATATTGATATAATATCAGTTTGTTTGCTTTATTATCGAGTTTTTCTTTTTGGAAGAATAATATTTAAGTACTTCTTTATACCGACCACATTCAAAATTTTATTTATTCGAACTAAAATTATTCTACCCAAACAAAGAGTTTAATCTCCCAATTGATTAGCTAATTAATTTTCTTTTTTTTTTTTAAATTTAATTTTGAATAGAGCAAATGGCAATAGCAAAGGCGAAAGCCCCAGGGGAGCTATTGAACTGGGAAGACATACAGAAAATGAAGTATTCATGGAACGTGGCTTGTGAAGTGCTGAGACTTGCTTCCCCACTCCAAGGGGCCTTTAGAGAAGCCATAAACGACTTCGTTTTCAATGGCTTCTATATTCCCAAGGGTTGGAAGGTAATTTCACTCCTACACATCGTCGTACCTTTTTATTATTTTTTATTTTTTAATACAACATGTGGGGTGGGGATTCGAATTCACAATCTTTTAGTTGGAGATATGTATCAATTATATGATACAACTGAGCTATACTTGCTTTGACAATACATTATTGACCTCGGTACATATTTCAACGTCTTAATTGTTTATCTTAGGACGTAGGTTTGAATAACTGCTCAAAAGTTAAAAGCTAGATATTTTTTTAAAGAGGAACATATTTTTGTATTTTATCTTTTTTATGCATTATACTTTTTCAAATATAAAAATTTAGGAGGGAGAGAAATTATGGAGAAAATTTGACACTCTACCCTTCATATATCAATTTTTTTATTTCAGGAAGAACCAAAAAAATGGGCAATTACGTTTATGTCTCCCCAGTATTTGTGATGTGATATTGAAATTAATTAATACTAAAGTAAATTATAATCTTATTTTATTCCGGTTATTTGGAGCTAGATGTGATGTGAAACTCTGAATTATGTGTTTGATCAAAGCAATGTCTAATTATGAATTTTTCTATTTATTTTTAGAAAAAGGAAGTAAACTTTTCGATTGTTGCGTTTTGGGTTGCACGAAATTTAAGCTGCTACACGAATTTTGTTAGATTAAGAGCATTTTAAATTTATTTATTTTTTTGAATTAAAAGATTGTTTGCCCGAGAATTCGAACCATCTTAATCAGTTGAGCTAGGACGGTTTGTAATCATTTAAAAAAAAAATACCATTTTGATGATTGCAGTTATATTGGAGTTCAAACTCTACACACAAAAACGCAGATTACTTCCCCGACCCTTATACATTCGATCCATCAAGATTTGAGGGCAACGGTCCAGCGCCCTACACTTTCGTGCCCTTCGGCGGAGGCCCGAGAATGTGCCCTGGAAAAGAGTATGCCAGGCTCGAGATCCTCGTGTTCATGCACAACTTGGTCAAGAGATTCAAATGGGCGAAGATACTCGAGAAAGAGAAGATCGTCGTCGACCCGATGCCAATTCCTCAGAAAGGCCTCCCCGTTCGCCTTTTTCCTCATAAAACTGTCTCTGCTTAG

mRNA sequence

ATGGAGCATTTTCTGGTCTCTCTGTTGATATTTTTCGTGTCCGTTGTCTGCGTGGGCCTCTTCATCCTGTTCTACAAGCACAAATCCCCGTTCTCGTATCCAAACATTCCTCCCGGCGGCATCGGATTTCCGATCATCGGCGAGAGCCTGGAGTTCTTCTCCACCGGATGGAAAGGCGAGCCGGAGAAGTTCATCTTCGACCGCATCAACAAGTACAAGTCCCACATCTTCAAGACCTCCATCGTCGGCGCTCCGGCCGCCGTCTTCTGCGGCCCCACCTGCAACAAGTTCCTCTTCTCCAACGAGAACAAGCTCGTCACCGCCTGGTGGCCCGACTCCGTCAACAAAATCTTCCCCTCTTCCACCCAGACCAGCTCCATGGAGGAGGCCAAGAAACTCAAGAAGCTCCTCCCCCAGTTCCTTAAGCCCGAGGCTCTCCACCGTTACATCGGAATCATGGATGCCCTCGCCCACCGCCACTTCGCTTCCTCCTGGGAGAACAAGGACCAAGTCCTCGTCTTCCCCCTCGCCAAGAGCTTCACCTTCTCGTTGGCGTGCCGGCTGTTCGTGAGCGTGGAGGACGAAGCGCACGTGGAGAGGCTATCGGGGCCGTTTCAGCACATTGCGGCGGGGATCATATCGATGCCGATAGATTTTCCAGGAACGCCGTTCAATCGAGGAATCAAGGCGTCGAACTTCATCCGGAAGGAACTCCTGTCGATCGTGAAGCAGAGGAAAGTGGATCTGGCGGAAGGGAAGGCGTCGCCCACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGAGCGTACATGAACGAATTGGACATCGCCGATAAGATTCTCGGCCTCTTGATCGGCGGCCACGACACCGCCAGCGCCGCCTGCACCTTCATCGTCAAGTTCCTCGCTGAGCTTCCCCACATCTACGACGGCGTTTACAAAGAGCAAATGGCAATAGCAAAGGCGAAAGCCCCAGGGGAGCTATTGAACTGGGAAGACATACAGAAAATGAAGTATTCATGGAACGTGGCTTGTGAAGTGCTGAGACTTGCTTCCCCACTCCAAGGGGCCTTTAGAGAAGCCATAAACGACTTCGTTTTCAATGGCTTCTATATTCCCAAGGGTTGGAAGTTATATTGGAGTTCAAACTCTACACACAAAAACGCAGATTACTTCCCCGACCCTTATACATTCGATCCATCAAGATTTGAGGGCAACGGTCCAGCGCCCTACACTTTCGTGCCCTTCGGCGGAGGCCCGAGAATGTGCCCTGGAAAAGAGTATGCCAGGCTCGAGATCCTCGTGTTCATGCACAACTTGGTCAAGAGATTCAAATGGGCGAAGATACTCGAGAAAGAGAAGATCGTCGTCGACCCGATGCCAATTCCTCAGAAAGGCCTCCCCGTTCGCCTTTTTCCTCATAAAACTGTCTCTGCTTAG

Coding sequence (CDS)

ATGGAGCATTTTCTGGTCTCTCTGTTGATATTTTTCGTGTCCGTTGTCTGCGTGGGCCTCTTCATCCTGTTCTACAAGCACAAATCCCCGTTCTCGTATCCAAACATTCCTCCCGGCGGCATCGGATTTCCGATCATCGGCGAGAGCCTGGAGTTCTTCTCCACCGGATGGAAAGGCGAGCCGGAGAAGTTCATCTTCGACCGCATCAACAAGTACAAGTCCCACATCTTCAAGACCTCCATCGTCGGCGCTCCGGCCGCCGTCTTCTGCGGCCCCACCTGCAACAAGTTCCTCTTCTCCAACGAGAACAAGCTCGTCACCGCCTGGTGGCCCGACTCCGTCAACAAAATCTTCCCCTCTTCCACCCAGACCAGCTCCATGGAGGAGGCCAAGAAACTCAAGAAGCTCCTCCCCCAGTTCCTTAAGCCCGAGGCTCTCCACCGTTACATCGGAATCATGGATGCCCTCGCCCACCGCCACTTCGCTTCCTCCTGGGAGAACAAGGACCAAGTCCTCGTCTTCCCCCTCGCCAAGAGCTTCACCTTCTCGTTGGCGTGCCGGCTGTTCGTGAGCGTGGAGGACGAAGCGCACGTGGAGAGGCTATCGGGGCCGTTTCAGCACATTGCGGCGGGGATCATATCGATGCCGATAGATTTTCCAGGAACGCCGTTCAATCGAGGAATCAAGGCGTCGAACTTCATCCGGAAGGAACTCCTGTCGATCGTGAAGCAGAGGAAAGTGGATCTGGCGGAAGGGAAGGCGTCGCCCACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGAGCGTACATGAACGAATTGGACATCGCCGATAAGATTCTCGGCCTCTTGATCGGCGGCCACGACACCGCCAGCGCCGCCTGCACCTTCATCGTCAAGTTCCTCGCTGAGCTTCCCCACATCTACGACGGCGTTTACAAAGAGCAAATGGCAATAGCAAAGGCGAAAGCCCCAGGGGAGCTATTGAACTGGGAAGACATACAGAAAATGAAGTATTCATGGAACGTGGCTTGTGAAGTGCTGAGACTTGCTTCCCCACTCCAAGGGGCCTTTAGAGAAGCCATAAACGACTTCGTTTTCAATGGCTTCTATATTCCCAAGGGTTGGAAGTTATATTGGAGTTCAAACTCTACACACAAAAACGCAGATTACTTCCCCGACCCTTATACATTCGATCCATCAAGATTTGAGGGCAACGGTCCAGCGCCCTACACTTTCGTGCCCTTCGGCGGAGGCCCGAGAATGTGCCCTGGAAAAGAGTATGCCAGGCTCGAGATCCTCGTGTTCATGCACAACTTGGTCAAGAGATTCAAATGGGCGAAGATACTCGAGAAAGAGAAGATCGTCGTCGACCCGATGCCAATTCCTCAGAAAGGCCTCCCCGTTCGCCTTTTTCCTCATAAAACTGTCTCTGCTTAG

Protein sequence

MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGEPEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPSSTQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSFTFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLSIVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACTFIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPFGGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKTVSA
Homology
BLAST of Moc06g14320 vs. NCBI nr
Match: XP_022158061.1 (beta-amyrin 28-oxidase [Momordica charantia])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.2e-288
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480

Query: 481 VSA 484
           VSA
Sbjct: 481 VSA 483

BLAST of Moc06g14320 vs. NCBI nr
Match: XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 857.8 bits (2215), Expect = 4.4e-245
Identity = 402/482 (83.40%), Postives = 439/482 (91.08%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           MEHFL+SLLIFF+  + + LFILF+ HKS FSYPN+PPG IG PI+GESLEF STGWKGE
Sbjct: 1   MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR++KYKS +FKTSIVG PAA+FCG  CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           STQTSS EEAKKLKK LPQFLKPEAL RYIG+MD LA RHFAS WEN+++VLVFPLAKSF
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLFVSVEDE HV+RLSGPF+HIAAGIIS+PID PGTPFNR IKAS FIRKEL++
Sbjct: 181 TFSLACRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVA 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IVK+R+ DLAEGKAS TQDILSHMLLTCDENG +MNE DIADKILGLLIGGHDTAS ACT
Sbjct: 241 IVKKRRWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIY GVY EQM IAK KAPGE L WEDI+KMKYSWNV CEVLRLASP QG
Sbjct: 301 FIVKFLAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREA+ DFVFNGFYIPKGWKLYWS+NSTHK+ +YFP+PY F+P+RFEGNGP PYTFVPF
Sbjct: 361 AFREALTDFVFNGFYIPKGWKLYWSANSTHKSPEYFPEPYKFEPARFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKWAK+LEKE I+VDPMPIPQKGLP+RLFPH+ 
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPMPIPQKGLPIRLFPHQP 480

Query: 481 VS 483
           +S
Sbjct: 481 LS 482

BLAST of Moc06g14320 vs. NCBI nr
Match: XP_022960871.1 (beta-amyrin 28-oxidase [Cucurbita moschata])

HSP 1 Score: 857.4 bits (2214), Expect = 5.8e-245
Identity = 407/482 (84.44%), Postives = 438/482 (90.87%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           M  FL+SLLI F+S++ + LF+LFYKHKS FSYPN PPG IG PIIGESLEF STGWKGE
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS IFKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           ST+ +S E A +LKKLLP+FLKPEAL RYIG+MD LA RHFASSWEN++QVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLFVS+ED  HVERLS PFQ IAAGIISMPID PGTPFNR IKASNF+RKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IV+QRK DLAEGKASPTQDILSHMLLTCDE G YMNE  IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIV +LAELP IYDGVY+EQM IAKAKA GELL WEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREAIN FVFNGFYIPKGWK+YWSSNSTHKN  YFP+PY FDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+L+KEKI+VDP+PIP+KGLPVRLFPH T
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTT 480

Query: 481 VS 483
           +S
Sbjct: 481 LS 482

BLAST of Moc06g14320 vs. NCBI nr
Match: KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 857.4 bits (2214), Expect = 5.8e-245
Identity = 407/482 (84.44%), Postives = 438/482 (90.87%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           M  FL+SLLI F+S++ + LF+LFYKHKS FSYPN PPG IG PIIGESLEF STGWKGE
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS IFKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           ST+ +S E A +LKKLLP+FLKPEAL RYIG+MD LA RHFASSWEN++QVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLFVS+ED  HVERLS PFQ IAAGIISMPID PGTPFNR IKASNF+RKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IV+QRK DLAEGKASPTQDILSHMLLTCDE G YMNE  IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIVK+LAELP IYDGVY+EQM IAKAKA GELL WEDIQKMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREAIN FVFNGFYIPKGWK+YWSSNSTHKN  YFP+PY FDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+L+KEKI+VDP+PIP+KGLPVRLFPHK 
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480

Query: 481 VS 483
           +S
Sbjct: 481 LS 482

BLAST of Moc06g14320 vs. NCBI nr
Match: XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 856.3 bits (2211), Expect = 1.3e-244
Identity = 406/482 (84.23%), Postives = 437/482 (90.66%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           M  FL+SLLI F+S + + LF+LFYKHKS FSYPN PPG IG PIIGESLEF STGWKGE
Sbjct: 1   MADFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS IFKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           ST+ +S E A +LKKLLP+FLKPEAL RYIG+MD LA RHFASSWEN++QVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLFVS+ED  HVERLS PFQ+IAAGIISMPID PGTPFNR IKASNF+RKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IV+QRK DLAEGKASPTQDILSHMLLTCDE G YMNE  IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIVK+LAELP IYD VY+EQM IAKAK  GELL WEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFRE INDFVFNGFYIPKGWKLYWSSNSTHKN  YFP+PY FDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+++KEKI+VDP+PIP+KGLPVRLFPHK 
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPHKY 480

Query: 481 VS 483
           +S
Sbjct: 481 LS 482

BLAST of Moc06g14320 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 1.6e-221
Identity = 358/479 (74.74%), Postives = 417/479 (87.06%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           ME F +SLL+ FV  V +GL +LFYKH+S F+ PN+PPG IG+P++GESLEF STGWKG 
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDRI+KY S +FKTS++G PAAVF G   NKFLFSNENKLV AWWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           STQTSS EEAKK++KLLPQF KPEAL RYIGIMD +A RHFA SW+N+D+V+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TF LACRLF+S+ED AHV +   PF  +A+G+I++PID PGTPF+R IKASNFIRKEL +
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           I+KQRK+DLAEGKAS  QDILSHMLL  DE+G +MNE++IADKILGLLIGGHDTASAA T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           F++K++AELPHIY+ VY+EQM IA +KAPGELLNW+D+QKM+YSWNVACEV+RLA PLQG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREAI DFVFNGF IPKGWKLYWS+NSTHK+ + FP P  FDP+RFEG+GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHK 480
           GGGPRMCPGKEYARLEILVFMHN+VKRFKW K+L  EKI+VDPMP+P KGLPVRL P K
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPRK 479

BLAST of Moc06g14320 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 764.6 bits (1973), Expect = 6.7e-220
Identity = 357/479 (74.53%), Postives = 414/479 (86.43%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           ME F +SLL+  V  V + L++L YKH+S F+ PN+PPG IG+P++GESLEF STGWKG 
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDRI+KY S +FKTS++G PAAVF G   NKFLFSNENKLV AWWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           STQTSS EEAKK++KLLPQFLKPEAL RY GIMD +A RHFA SW+N+D+V+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TF LACRLF+S+ED AHV +   PF  +A+G+I++PID PGTPF+R IKASNFIRKEL +
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           I+KQRK+DLAE KAS TQDILSHMLL  DE+G +MNE+ IADKILGLLIGGHDTAS+A T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           F+VK++AELPHIY+ VYKEQM IA +KAPGELLNW+D+QKM+YSWNVACEV+RLA PLQG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREAI DFVFNGF IPKGWKLYWS+NSTHK+ + FP P  FDP+RFEG GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHK 480
           GGGPRMCPGKEYARLEIL+FMHNLVKRFKW K+L  EKI+VDPMP+P KGLPVRL PHK
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPHK 479

BLAST of Moc06g14320 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.5e-216
Identity = 352/478 (73.64%), Postives = 408/478 (85.36%), Query Frame = 0

Query: 3   HFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGEPE 62
           +F +SLL+ FV+ + + LF +FYK KSP    N+PPG +G+PIIGESLEF STGWKG PE
Sbjct: 4   NFYLSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPE 63

Query: 63  KFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPSST 122
           KFIFDR+ KY S +FKTSIVG    V CG   NKFLFSNENKLVTAWWPDSVNKIFP+++
Sbjct: 64  KFIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTS 123

Query: 123 QTSSM-EEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSFT 182
             S++ EE+ K++KLLPQF KPEAL RY+G+MD +A RHF + W+NK+++ V+PLAK +T
Sbjct: 124 LDSNLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYT 183

Query: 183 FSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLSI 242
           F LACRLF+SVEDE HV + S PFQ IAAGIIS+PID PGTPFN+ IKASNFIRKEL+ I
Sbjct: 184 FLLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKI 243

Query: 243 VKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACTF 302
           +KQR+VDLAEG ASPTQDILSHMLLT DENG  MNEL+IADKILGLLIGGHDTAS ACTF
Sbjct: 244 IKQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTF 303

Query: 303 IVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGA 362
           +VK+L ELPHIYD VY+EQM IAK+K  GELLNW+D++KMKYSWNVACEV+RL+ PLQG 
Sbjct: 304 LVKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGG 363

Query: 363 FREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPFG 422
           FREAI DF+FNGF IPKGWKLYWS+NSTHKNA+ FP P  FDP+RFEGNGPAPYTFVPFG
Sbjct: 364 FREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFG 423

Query: 423 GGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHK 480
           GGPRMCPGKEYARLEILVFMHNLVKRFKW K++  EKI+VDP PIP K LP+RL+PHK
Sbjct: 424 GGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHK 478

BLAST of Moc06g14320 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 7.9e-213
Identity = 351/480 (73.12%), Postives = 401/480 (83.54%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKS-PFSYPNIPPGGIGFPIIGESLEFFSTGWKG 60
           M+ F V LL  FV  V +  + LFYK KS   S   +PPG  G+P+IGES EF STGWKG
Sbjct: 1   MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60

Query: 61  EPEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFP 120
            PEKFIFDR+ KY S++FKTS+ G PAA FCG  CNKFLFSNENKLV AWWPDSVNK+FP
Sbjct: 61  YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120

Query: 121 SSTQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKS 180
           SSTQTSS EEA K++KLLP FLKPEAL RY+G+MD +A +HF S WENK +VLVFPLAK+
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180

Query: 181 FTFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELL 240
           +TF +AC++FVSVE+   V +L  PF  IA+GIIS+PID PGTPFN  IK+S  +R +L+
Sbjct: 181 YTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLM 240

Query: 241 SIVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAAC 300
            I+KQRK DL EGKASPTQDILSHMLLT DENG +M E DIADKILGLLIGGHDTAS+AC
Sbjct: 241 GIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSAC 300

Query: 301 TFIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQ 360
           TF+VKFLAELP IY+GVYKEQM IAK+K  GELLNWEDIQKMKYSWNVACEVLRLA PLQ
Sbjct: 301 TFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQ 360

Query: 361 GAFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVP 420
           GAFREA+ DF +NGF IPKGWKLYWS+NSTH+N++ FP+P  FDPSRFEG GP PY+FVP
Sbjct: 361 GAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVP 420

Query: 421 FGGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHK 480
           FGGGPRMCPGKEYARLEILVFMH++VKRFKW K++  EKIVV+PMPIP KGLPVRLFPHK
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480

BLAST of Moc06g14320 vs. ExPASy Swiss-Prot
Match: A0A3Q7HBJ5 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 3.0e-212
Identity = 344/473 (72.73%), Postives = 401/473 (84.78%), Query Frame = 0

Query: 10  IFFVSVVCVGLF----ILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGEPEKFI 69
           + +V +VCV +F    +L YK KS      +PPG  G+P+ GESLEF S+GWKG PEKFI
Sbjct: 3   LLYVCLVCVFVFLVSLLLLYKKKSG---EGLPPGKTGWPVFGESLEFLSSGWKGHPEKFI 62

Query: 70  FDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPSSTQTS 129
           FDR+ KY S +FKT ++G  AAVFCG + NKFLFSNENKLV AWWP+SVNK+FPSSTQTS
Sbjct: 63  FDRVAKYSSSVFKTHLLGEEAAVFCGASANKFLFSNENKLVQAWWPNSVNKVFPSSTQTS 122

Query: 130 SMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSFTFSLA 189
           S EEA K++K+LP F KPEAL RY+GIMD +  RHFA+ WENK+QV+VFPL K +TF LA
Sbjct: 123 SKEEAIKMRKMLPNFFKPEALQRYVGIMDHITQRHFATGWENKEQVVVFPLTKRYTFWLA 182

Query: 190 CRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLSIVKQR 249
           CRLF+SVED  HV + + PF  +A+G+IS+PID PGTPFNR IKASNFIRKEL+ I+KQR
Sbjct: 183 CRLFLSVEDPKHVAKFADPFDVLASGLISIPIDLPGTPFNRAIKASNFIRKELVRIIKQR 242

Query: 250 KVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACTFIVKF 309
           K+DL EGK S TQDILSHMLLTCDENG ++ +LDIADKILGLLIGGHDTAS+AC+FIVK+
Sbjct: 243 KIDLGEGKVSSTQDILSHMLLTCDENGKFLGDLDIADKILGLLIGGHDTASSACSFIVKY 302

Query: 310 LAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFREA 369
           LAELPHIY  VY EQM IAK+K PGELL WEDIQKMKYSWNVACEVLRLA PLQGAFREA
Sbjct: 303 LAELPHIYQRVYTEQMEIAKSKGPGELLRWEDIQKMKYSWNVACEVLRLAPPLQGAFREA 362

Query: 370 INDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPFGGGPR 429
           ++DF+FNGFYIPKGWK+YWS+NSTHK  ++FPDP  FDPSRFEG+GPAPYTFVPFGGGPR
Sbjct: 363 LSDFMFNGFYIPKGWKIYWSANSTHKREEFFPDPEKFDPSRFEGSGPAPYTFVPFGGGPR 422

Query: 430 MCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPH 479
           MCPGKEYARLEILVFMH+LVKRFK+ KI+  EKI+V+PMPIP  GLP+RL+PH
Sbjct: 423 MCPGKEYARLEILVFMHHLVKRFKFEKIIPHEKIIVNPMPIPANGLPLRLYPH 472

BLAST of Moc06g14320 vs. ExPASy TrEMBL
Match: A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)

HSP 1 Score: 1000.7 bits (2586), Expect = 2.0e-288
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480

Query: 481 VSA 484
           VSA
Sbjct: 481 VSA 483

BLAST of Moc06g14320 vs. ExPASy TrEMBL
Match: A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.8e-245
Identity = 407/482 (84.44%), Postives = 438/482 (90.87%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           M  FL+SLLI F+S++ + LF+LFYKHKS FSYPN PPG IG PIIGESLEF STGWKGE
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS IFKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           ST+ +S E A +LKKLLP+FLKPEAL RYIG+MD LA RHFASSWEN++QVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLFVS+ED  HVERLS PFQ IAAGIISMPID PGTPFNR IKASNF+RKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IV+QRK DLAEGKASPTQDILSHMLLTCDE G YMNE  IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIV +LAELP IYDGVY+EQM IAKAKA GELL WEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREAIN FVFNGFYIPKGWK+YWSSNSTHKN  YFP+PY FDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+L+KEKI+VDP+PIP+KGLPVRLFPH T
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTT 480

Query: 481 VS 483
           +S
Sbjct: 481 LS 482

BLAST of Moc06g14320 vs. ExPASy TrEMBL
Match: A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.1e-245
Identity = 410/484 (84.71%), Postives = 440/484 (90.91%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           ME FL+SLLI F+S + + LF+LFYKHKS FSYPN PPG IG PIIGESLEF STGWKGE
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS IFKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           ST+ +S E A +LKKLLP+FLKPEAL RYIG+MD LA RHFASSWEN++ VLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLF+S+ED  HVERLS PFQ+IAAGIISMPID PGTPFNR IKASNF+RKELLS
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IV+QRK DLAEGKASPTQDILSHMLLTCDE G YMNE  IAD ILGLLIGGHDTASAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAI--AKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPL 360
           FIVK+LAELP IYD VY+EQM I  AKAKA GELL WEDIQKMKYSWNVACEVLRLASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360

Query: 361 QGAFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFV 420
           QGAFREAINDFVFNGF IPKGWKLYWSSNSTHKN  YFP+PY FDPSRFEGNGPAPYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420

Query: 421 PFGGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPH 480
           PFGGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+LEKEKI+VDP+PIP+KGLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480

Query: 481 KTVS 483
           KT+S
Sbjct: 481 KTLS 484

BLAST of Moc06g14320 vs. ExPASy TrEMBL
Match: A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.9e-242
Identity = 398/482 (82.57%), Postives = 433/482 (89.83%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           MEHFL+SLLI     + + LFILF+ HKS FSYPN PPG IG PI+GESLEF S+GW+G 
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           +TQTSS EEAKKLKKLLPQFLKPEAL RYIGIMD LA RHF S W+N+++VLVFPLAKSF
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFSLACRLF+SVEDE HVERLSGPF+HIAAGIISMPID PGTPFNR IKAS FIRKE+++
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IV+QRK DLAEGKA  TQDILSHMLLTCDENG YMNE DI DKILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYD VY EQM IA+AKA GE L WEDI+KMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREA+NDFVFNGF+IPKGWKLYWS+NSTHKN +YFP+PY F+P RFEGNGP PYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+LE E I+V+PMPIPQKGLPVRLFPH+ 
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 481 VS 483
           +S
Sbjct: 481 LS 482

BLAST of Moc06g14320 vs. ExPASy TrEMBL
Match: A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 7.9e-240
Identity = 394/482 (81.74%), Postives = 432/482 (89.63%), Query Frame = 0

Query: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
           ME FL+SLLI     + + LFILF+ HKS FSYPN PPG IG PI+GES+EF S+GWKG 
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
           +TQTS+ EEAKKLKKLLPQFLKPEAL RYIGIMD LA RHF S W+N+++VLVFPLAKSF
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
           TFS+ACRLF+SVEDE HVERLSGPF+HIAAGIISMPID PGTPFNR IKAS FIRKE+++
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
           IV+QRK DLAEGKA  TQDILSHMLLTCDENG YMNE DI DKILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYD VY EQM IA+AKA GE L WEDI+KMKYSWNVACEVLR+ASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360

Query: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
           AFREA++DFVFNGF+IPKGWKLYWS+NSTHKN +YFP+PY FDP RFEGNGP PYTFVPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+LE E I+V+PMPIPQKGLPVRLFPH+ 
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 481 VS 483
           +S
Sbjct: 481 LS 482

BLAST of Moc06g14320 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 630.9 bits (1626), Expect = 8.2e-181
Identity = 296/478 (61.92%), Postives = 361/478 (75.52%), Query Frame = 0

Query: 4   FLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGEPEK 63
           ++  ++I F+S + + L +L  KH S FSYPN+PPG  G P+IGES  F S G +G PEK
Sbjct: 2   YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEK 61

Query: 64  FIFDRINKYKSH---IFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 123
           FI DR+ ++ S    +FKT + G+P AV  G + NKFLF+NENKLV +WWPDSVNKIFPS
Sbjct: 62  FITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPS 121

Query: 124 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 183
           S QTSS EEA+KL+ LL QF+KPEAL RY+G+MD +A RHF + W N+DQV+VFPL K F
Sbjct: 122 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKF 181

Query: 184 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 243
           TFS+ACR F+S+ED A V +L   F  +A GI S+PID PGT FNR IKAS  +RKE+ +
Sbjct: 182 TFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSA 241

Query: 244 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 303
           IV+QRK +L  GKA    DILSHML+   E      + D+ADKI+GLLIGGHDTAS  CT
Sbjct: 242 IVRQRKEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCT 301

Query: 304 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 363
           F+V +LAE PH+Y  V +EQ  I K K   E L WEDI+KM+YSWNVACEV+R+  PL G
Sbjct: 302 FVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSG 361

Query: 364 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 423
            FREAI+ F F GFYIPKGWKLYWS+ +TH N DYFP+P  F+P+RFEG+GP PYT+VPF
Sbjct: 362 TFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPF 421

Query: 424 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKE-KIVVDPMPIPQKGLPVRLFP 478
           GGGPRMCPGKEYARLEIL+FMHNLV RFKW K+   E KIVVDP+PIP KGLP+R+FP
Sbjct: 422 GGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 475

BLAST of Moc06g14320 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 364.0 bits (933), Expect = 1.9e-100
Identity = 173/311 (55.63%), Postives = 221/311 (71.06%), Query Frame = 0

Query: 6   VSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGEPEKFI 65
           ++++  F+S +   L     KH S F YPN+PPG IGFP+IGE+L F S G +G PEKF+
Sbjct: 3   LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62

Query: 66  FDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPSSTQTS 125
            DR+  + S IFKT + G+P AV  G + NKFLF+NENKLV +WWPDSVNKIFPSSTQTS
Sbjct: 63  TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122

Query: 126 SMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSFTFSLA 185
           S EEA K + LL   +KPEAL RY+G+MD +A +HF + W N+DQ++VFPL K FTFS+A
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182

Query: 186 CRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLSIVKQR 245
           CRLF+S++D   V +L  PF  +  G+ S+PID PGT FNR IKAS  + KE+ +I++QR
Sbjct: 183 CRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQR 242

Query: 246 KVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACTFIVKF 305
           K +L  GK S  QDILSHML+   E      + D+ADKI+ LLIGGHDT S  CTF+V +
Sbjct: 243 KEELKAGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNY 302

Query: 306 LAELPHIYDGV 317
           LAE PHIY  V
Sbjct: 303 LAEFPHIYQRV 309

BLAST of Moc06g14320 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 270.0 bits (689), Expect = 3.7e-72
Identity = 154/484 (31.82%), Postives = 254/484 (52.48%), Query Frame = 0

Query: 3   HFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIP------PGGIGFPIIGESLEFFSTG 62
           +F++S +   ++         F K    F    I       PG +G P IGE+++F+   
Sbjct: 6   NFVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQ 65

Query: 63  WKGEP-EKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWP-DSV 122
                 E F+  RI K+  +IFKT I+G+P  V  G   N+ + SNE  LV + WP  SV
Sbjct: 66  KSNRVFEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSV 125

Query: 123 NKIFPSSTQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVF 182
             +  +       E+ + L+ ++   L    L   I  +      H  + W  K+++ ++
Sbjct: 126 QLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLY 185

Query: 183 PLAKSFTFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFI 242
             AK  TF++       ++ E  +  +   F+ +  G+ ++P++FP + F R  KA   I
Sbjct: 186 RSAKVLTFTVVFECLYGIKVEIGMLEV---FERVLEGVFALPVEFPCSKFARAKKARLEI 245

Query: 243 RKELLSIVKQRKVDL-AEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHD 302
              L+  V++++ ++  EG   P   + S ++    +    + E ++ D ++ L+   HD
Sbjct: 246 ETFLVGKVREKRREMEKEGAEKPNTTLFSRLVEELIK--GVITEEEVVDNMVLLVFAAHD 305

Query: 303 TASAACTFIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLR 362
           T S A +   K LA+ P   D + +E   I   K  GE L  ED++KMKYSW V  E +R
Sbjct: 306 TTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMR 365

Query: 363 LASPLQGAFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPA 422
           L+ P+ G+FR+A+ D  + G+ IPKGWK+ W++  TH N + F DP +FDP+RF+    A
Sbjct: 366 LSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKPIQA 425

Query: 423 PYTFVPFGGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPV 478
            YT++PFGGGPR+C G + A++ ILVFMH +V  F W+ +   E I +DP+P P  G+P+
Sbjct: 426 -YTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPI 482

BLAST of Moc06g14320 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 263.1 bits (671), Expect = 4.5e-70
Identity = 152/461 (32.97%), Postives = 250/461 (54.23%), Query Frame = 0

Query: 16  VCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGEPEKFIFDRINKYKSH 75
           +C+  FI   +  S    P +PPG +G+P +GE+ + +S     +P  F   +  +Y S 
Sbjct: 17  LCLLRFIAGVRRSSSTKLP-LPPGTMGYPYVGETFQLYSQ----DPNVFFAAKQRRYGS- 76

Query: 76  IFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIF-PSSTQTSSMEEAKKLK 135
           +FKT ++G P  +   P   KF+   ++ L    +P S  ++    +      +   KL+
Sbjct: 77  VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLR 136

Query: 136 KLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSFTFSLACRLFVSVED 195
           KL+ +   P+A+   +  ++++A     +SW+   Q+  +   K++TF++A    +  ++
Sbjct: 137 KLVLRAFMPDAIRNMVPHIESIAQESL-NSWDG-TQLNTYQEMKTYTFNVALISILGKDE 196

Query: 196 EAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLSIVKQRKVDLAEGKA 255
             + E L   +  +  G  SMPI+ PGT F++ +KA   + + L +I+ +R+ +      
Sbjct: 197 VYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQN-----P 256

Query: 256 SPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACTFIVKFLAELPHIYD 315
           S   D+L   +    E+ A + +  IAD I+G++    DT ++  T+I+K+LA+ P + +
Sbjct: 257 SSHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLE 316

Query: 316 GVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFREAINDFVFNGF 375
            V +EQMAI K K  GE L WED +KM  ++ V  E LR A+ L   FREA+ D  + G+
Sbjct: 317 AVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGY 376

Query: 376 YIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPFGGGPRMCPGKEYAR 435
            IPKGWK+     + H NAD F DP  FDPSRFE   P P TF+PFG G   CPG E A+
Sbjct: 377 LIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE-VAPKPNTFMPFGSGIHSCPGNELAK 436

Query: 436 LEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRL 476
           LEI V +H+L  +++W+ +   + I   P  +PQ GLP+ L
Sbjct: 437 LEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459

BLAST of Moc06g14320 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 260.4 bits (664), Expect = 2.9e-69
Identity = 156/479 (32.57%), Postives = 252/479 (52.61%), Query Frame = 0

Query: 2   EHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGEP 61
           E  L++L I  V VV +  + L +K +       +PPG +G P IGE+L  ++      P
Sbjct: 19  EPALITLTIVVVVVVLLFKWWLHWKEQR----LRLPPGSMGLPYIGETLRLYTE----NP 78

Query: 62  EKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNK-IFPS 121
             F   R NKY   IFKT I+G P  +   P   + +  ++  L    +P S  + I P 
Sbjct: 79  NSFFATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPE 138

Query: 122 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 181
           +           LK+L+     P AL   +  ++ L  +   SSW ++  +      K +
Sbjct: 139 ALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTL-SSWTSQKSINTLEYMKRY 198

Query: 182 TFSLA-CRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELL 241
            F +A    F   E+   ++ +   +Q +  G  SMP+D PGT F++ +KA   + +EL 
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 258

Query: 242 SIVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAAC 301
            ++++R+ +  E        +L  +L   D+    +++  IAD I+G++    DT ++  
Sbjct: 259 KVIEKRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318

Query: 302 TFIVKFLAELPHIYDGVYKEQMAI-AKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPL 361
           T+++K+L + P++   V +EQ +I  K K     ++WED +KM  +  V  E LR AS L
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378

Query: 362 QGAFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFV 421
              FREA+ D  ++G+ IPKGWK+       H ++++FPDP  FDPSRFE   P PYT++
Sbjct: 379 SFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYM 438

Query: 422 PFGGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFP 478
           PFG G   CPG E A+LE+L+ +H+L   F+W  I ++E I   P P+P+KGLP+R+ P
Sbjct: 439 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158061.14.2e-288100.00beta-amyrin 28-oxidase [Momordica charantia][more]
XP_038877759.14.4e-24583.40LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida][more]
XP_022960871.15.8e-24584.44beta-amyrin 28-oxidase [Cucurbita moschata][more]
KAG6590124.15.8e-24584.44Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023516848.11.3e-24484.23beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
F6H9N61.6e-22174.74Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1[more]
A5BFI46.7e-22074.53Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2[more]
Q2MJ201.5e-21673.64Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV... [more]
A0A0S2II387.9e-21373.13Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... [more]
A0A3Q7HBJ53.0e-21272.73Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1DZX72.0e-288100.00beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1[more]
A0A6J1H8T42.8e-24584.44beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1[more]
A0A6J1JJQ78.1e-24584.71beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1[more]
A0A1S3B8H44.9e-24282.57beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1[more]
A0A0A0LXN37.9e-24081.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36110.18.2e-18161.92cytochrome P450, family 716, subfamily A, polypeptide 1 [more]
AT5G36140.11.9e-10055.63cytochrome P450, family 716, subfamily A, polypeptide 2 [more]
AT2G42850.13.7e-7231.82cytochrome P450, family 718 [more]
AT5G45340.14.5e-7032.97cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT2G29090.12.9e-6932.57cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 417..427
score: 52.97
coord: 427..450
score: 30.32
coord: 279..296
score: 25.34
coord: 385..409
score: 27.26
IPR001128Cytochrome P450PRINTSPR00385P450coord: 290..307
score: 27.61
coord: 418..427
score: 58.31
coord: 427..438
score: 38.09
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..457
e-value: 8.0E-54
score: 183.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..477
e-value: 5.2E-109
score: 367.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 31..477
NoneNo IPR availablePANTHERPTHR24286:SF297BETA-AMYRIN 28-OXIDASE-LIKEcoord: 11..476
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 11..476
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 420..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g14320.1Moc06g14320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen