Moc06g12140 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g12140
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	isoleucine N-monooxygenase 2-like
Location	chr6: 9284382 .. 9286323 (+)
RNA-Seq Expression	Moc06g12140
Synteny	Moc06g12140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGCAAGCTTTCTTATTTATCACTCAATTTACATATCAAAACTATGCAATAGCCATGACCAAAACCTTCACCTTCTCCCTTCTCATGGCTTTCATTATTTCTCTCACCTTTTCTCAATGCTACCGGAAAAGCAAGCTCTCCGTCCCCCGCCATGCAGCTCTCCCGCCTGGCCCGACACCGTGGCCCTTCGTCGGCTGCCTCCCGGAAATGTGGACGAATAACACACTGCCACACCAATGGATTCATGCAATAATGAAGCAGTACGACACTGAAATAGCATGCATTCGTCTCGGGAATACCTACGTCATTCCAGTGACTTCTCCACAACTTGCTCTCGAATTTCTAAAGACCCATGATTCTGTCTTTGCATCACGACCAATGCTCGACACTACCAAAATCATAACCCGTGGATTCCTTACCACTGCTCTTTCGCCATTGGGAGAGCAATGGAAGAAGATGCGCAAAATACTTACTTCACAAATACTTAATCCCTCCACCCTCCACCAAATGCTGGCTCATAGAACACACGAAGCCGACACTCTTTTACGCTACATTTTCAGTCTCACCAAGTCGAAAGGTGCGGCGGTCGTCAACTTGAGAAGCGTAACTCTACATTATTGTGGCAACGTTATTAGAAGAGTGCTATTCAACAGAAGATACTACGGCGCTGGCCAAGAGGATGGAGGGCCAACTTTTGAAGAAGAAGAGCACATTCAAGCACTGCTAGCATTGCTCAAGTATATCAATGCCTTTTCTGTATCGGATTTCATACCTTGTTTGAGGCCGTTTGATGTGGACAAACATCAAAAGATTGTAAAAGAAGGTTTGAAAGTGGTGAGAAAGCATGACGAACCGATTGTGGATGAGAGAGTGCAACAATGGAAATATGGAAAGAGGAAAGAGATCGAAGATTTTCTTGATATCTTTATCGCGCTTAAATATGAAAATGGAAAATCGTTGCTATCGATCGAAGAGATTAAAGCTGAAATCACGGTAAGAATTCTTGTCAAACATATAAGTATAATATTTGTTTAGGTACTTACCCATCATCACTCTTTTTGGTTAACTAAACTTTTTTACCAGTAGAGGAGAGGGAGGGAGAAAATATATATATTTCCTTTTAAAAGAAACGATAAGATTGATAAATTTTTATCCTATAAAATTTAAGCAGTTCTACCTACCACAATTAAAAGTTTAACAATTCTTTATTCTATTTTTTCACTCTTTTCTTTTTTCATTAAATCTCTCTCAGTTGTCAATTTTTCAATAGAAAATACATACGTCTTGATTAGTGGTGACCAATATTATCCTCACACCATTAAATGTTTGACAACTAAAAAAAGGAAATGCAAAAACATAATTTTTTTAAAAAAATTTCATGGGATAGATAAGTTGTTAGCTCTACACATGCATGCATATGACAAATTAACTTCAACCTTTCTCATCGTTCATAATAACAGGAGCTACAAATGGCAACGATTGATAATCCTTCAAATGCAGTAGAATGGGCAATGGCAGAATTACTCAATCAACCAAAAATTCTCCAGAAAGCAATTGAAGAACTAGATAGGATTGTTGAAAGGGATAGACTTGTTCAAGAATATGATATCCCACAACTTAAGTATCTTATTGCTTGTGTTAGATTTATTGCTGTGTTAGAGAAAGAATCCCACAACTTAAGTATCTTATTGCTTGTGTTAGATTTATTGCTTGTGTTAGAGAATCTTTCCGACTCCATCCATTTACACCATTCAATGTTCCTCATGTCTCAAATTCCAATGTCATTGTAGCTGGATACTTTATCCCAAAAGGCAGCCAAGTCCTACTTAGTCGAATCGGTCTCGGTCGAAACCTGAAGATTTGGAAGGATCCAATACGATTCGATCCAGAACGCCATCTCAAGGATAAGACGATGGGTGACATGGGATTATCATAA

mRNA sequence

ATGGCCATGCAAGCTTTCTTATTTATCACTCAATTTACATATCAAAACTATGCAATAGCCATGACCAAAACCTTCACCTTCTCCCTTCTCATGGCTTTCATTATTTCTCTCACCTTTTCTCAATGCTACCGGAAAAGCAAGCTCTCCGTCCCCCGCCATGCAGCTCTCCCGCCTGGCCCGACACCGTGGCCCTTCGTCGGCTGCCTCCCGGAAATGTGGACGAATAACACACTGCCACACCAATGGATTCATGCAATAATGAAGCAGTACGACACTGAAATAGCATGCATTCGTCTCGGGAATACCTACGTCATTCCAGTGACTTCTCCACAACTTGCTCTCGAATTTCTAAAGACCCATGATTCTGTCTTTGCATCACGACCAATGCTCGACACTACCAAAATCATAACCCGTGGATTCCTTACCACTGCTCTTTCGCCATTGGGAGAGCAATGGAAGAAGATGCGCAAAATACTTACTTCACAAATACTTAATCCCTCCACCCTCCACCAAATGCTGGCTCATAGAACACACGAAGCCGACACTCTTTTACGCTACATTTTCAGTCTCACCAAGTCGAAAGGTGCGGCGGTCGTCAACTTGAGAAGCGTAACTCTACATTATTGTGGCAACGTTATTAGAAGAGTGCTATTCAACAGAAGATACTACGGCGCTGGCCAAGAGGATGGAGGGCCAACTTTTGAAGAAGAAGAGCACATTCAAGCACTGCTAGCATTGCTCAAGTATATCAATGCCTTTTCTGTATCGGATTTCATACCTTGTTTGAGGCCGTTTGATGTGGACAAACATCAAAAGATTGTAAAAGAAGGTTTGAAAGTGGTGAGAAAGCATGACGAACCGATTGTGGATGAGAGAGTGCAACAATGGAAATATGGAAAGAGGAAAGAGATCGAAGATTTTCTTGATATCTTTATCGCGCTTAAATATGAAAATGGAAAATCGTTGCTATCGATCGAAGAGATTAAAGCTGAAATCACGGAGCTACAAATGGCAACGATTGATAATCCTTCAAATGCAGTAGAATGGGCAATGGCAGAATTACTCAATCAACCAAAAATTCTCCAGAAAGCAATTGAAGAACTAGATAGGATTGTTGAAAGGGATAGACTTGTTCAAGAATATGATATCCCACAACTTAAGTATCTTATTGCTTGTGTTAGATTTATTGCTGTGTTAGAGAAAGAATCCCACAACTTAACTGGATACTTTATCCCAAAAGGCAGCCAAGTCCTACTTAGTCGAATCGGTCTCGGTCGAAACCTGAAGATTTGGAAGGATCCAATACGATTCGATCCAGAACGCCATCTCAAGGATAAGACGATGGGTGACATGGGATTATCATAA

Coding sequence (CDS)

ATGGCCATGCAAGCTTTCTTATTTATCACTCAATTTACATATCAAAACTATGCAATAGCCATGACCAAAACCTTCACCTTCTCCCTTCTCATGGCTTTCATTATTTCTCTCACCTTTTCTCAATGCTACCGGAAAAGCAAGCTCTCCGTCCCCCGCCATGCAGCTCTCCCGCCTGGCCCGACACCGTGGCCCTTCGTCGGCTGCCTCCCGGAAATGTGGACGAATAACACACTGCCACACCAATGGATTCATGCAATAATGAAGCAGTACGACACTGAAATAGCATGCATTCGTCTCGGGAATACCTACGTCATTCCAGTGACTTCTCCACAACTTGCTCTCGAATTTCTAAAGACCCATGATTCTGTCTTTGCATCACGACCAATGCTCGACACTACCAAAATCATAACCCGTGGATTCCTTACCACTGCTCTTTCGCCATTGGGAGAGCAATGGAAGAAGATGCGCAAAATACTTACTTCACAAATACTTAATCCCTCCACCCTCCACCAAATGCTGGCTCATAGAACACACGAAGCCGACACTCTTTTACGCTACATTTTCAGTCTCACCAAGTCGAAAGGTGCGGCGGTCGTCAACTTGAGAAGCGTAACTCTACATTATTGTGGCAACGTTATTAGAAGAGTGCTATTCAACAGAAGATACTACGGCGCTGGCCAAGAGGATGGAGGGCCAACTTTTGAAGAAGAAGAGCACATTCAAGCACTGCTAGCATTGCTCAAGTATATCAATGCCTTTTCTGTATCGGATTTCATACCTTGTTTGAGGCCGTTTGATGTGGACAAACATCAAAAGATTGTAAAAGAAGGTTTGAAAGTGGTGAGAAAGCATGACGAACCGATTGTGGATGAGAGAGTGCAACAATGGAAATATGGAAAGAGGAAAGAGATCGAAGATTTTCTTGATATCTTTATCGCGCTTAAATATGAAAATGGAAAATCGTTGCTATCGATCGAAGAGATTAAAGCTGAAATCACGGAGCTACAAATGGCAACGATTGATAATCCTTCAAATGCAGTAGAATGGGCAATGGCAGAATTACTCAATCAACCAAAAATTCTCCAGAAAGCAATTGAAGAACTAGATAGGATTGTTGAAAGGGATAGACTTGTTCAAGAATATGATATCCCACAACTTAAGTATCTTATTGCTTGTGTTAGATTTATTGCTGTGTTAGAGAAAGAATCCCACAACTTAACTGGATACTTTATCCCAAAAGGCAGCCAAGTCCTACTTAGTCGAATCGGTCTCGGTCGAAACCTGAAGATTTGGAAGGATCCAATACGATTCGATCCAGAACGCCATCTCAAGGATAAGACGATGGGTGACATGGGATTATCATAA

Protein sequence

MAMQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESHNLTGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS

Homology

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BLAST of Moc06g12140 vs. NCBI nr
Match: XP_022154369.1 (phenylalanine N-monooxygenase-like [Momordica charantia])

HSP 1 Score: 810.1 bits (2091), Expect = 9.9e-231
Identity = 406/407 (99.75%), Postives = 407/407 (100.00%), Query Frame = 0


Query: 1   MAMQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGP 60
           MAMQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGP
Sbjct: 1   MAMQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGP 60

Query: 61  TPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTH 120
           TPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTH
Sbjct: 61  TPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTH 120

Query: 121 DSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA 180
           DSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA
Sbjct: 121 DSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA 180

Query: 181 DTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHI 240
           DTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHI
Sbjct: 181 DTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHI 240

Query: 241 QALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGK 300
           QALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGK
Sbjct: 241 QALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGK 300

Query: 301 RKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKI 360
           RKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKI
Sbjct: 301 RKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKI 360

Query: 361 LQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESHNLT 408
           LQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESHNL+
Sbjct: 361 LQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESHNLS 407

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BLAST of Moc06g12140 vs. NCBI nr
Match: XP_022148957.1 (isoleucine N-monooxygenase 2-like [Momordica charantia])

HSP 1 Score: 616.7 bits (1589), Expect = 1.6e-172
Identity = 314/468 (67.09%), Postives = 375/468 (80.13%), Query Frame = 0


Query: 3   MQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPTP 62
           M+A LFI+Q TYQN   A+TKTFT  + MAF IS  FSQCYRK K SV     LPPGP P
Sbjct: 1   MEALLFISQLTYQNSPTAITKTFTLFVFMAF-ISAIFSQCYRKRKHSV---LQLPPGPNP 60

Query: 63  WPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDS 122
           WP VGCLP M    +  ++WIHA+MKQ+DTEIACIRLGNTYV+PVTSP+LA+EFLK HDS
Sbjct: 61  WPLVGCLPAMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVMPVTSPELAVEFLKRHDS 120

Query: 123 VFASRPML-DTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEAD 182
           VFASR  + +T  +++RG LTTA SPLG QWKKMR+IL S+I  PSTLHQML HRT+EAD
Sbjct: 121 VFASRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEAD 180

Query: 183 TLLRYIFSLTK----SKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEE 242
           TLLRYIFSLT     +K  AVVN+RSVT HYCGN+IRR+LFN+RYYG G+EDGGP+FEEE
Sbjct: 181 TLLRYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSFEEE 240

Query: 243 EHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWK 302
           EH QALL +L+++NAFS+SDF+PCL+PFD+D H+KI+K  LK + K+DEPI++ERVQQWK
Sbjct: 241 EHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQWK 300

Query: 303 YGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQ 362
            GKR+  ED LDI I+ K  NGKSLL+I+EIKA+ITELQ+ATIDNPSNAVEW MAELLNQ
Sbjct: 301 DGKREGAEDILDILISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAELLNQ 360

Query: 363 PKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVR-----------FIAVLEKESHNL 422
           PKILQKAIEELDR+V R+RLVQE DIP+LKYL AC R            I  +      +
Sbjct: 361 PKILQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVV 420

Query: 423 TGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS 455
            GYFIPKGS+V+LSR GLGRN +IW+DP+RFDPERHLKD+T+ ++GLS
Sbjct: 421 AGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETV-ELGLS 463

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BLAST of Moc06g12140 vs. NCBI nr
Match: XP_022148952.1 (isoleucine N-monooxygenase 2-like [Momordica charantia])

HSP 1 Score: 607.8 bits (1566), Expect = 7.5e-170
Identity = 316/469 (67.38%), Postives = 371/469 (79.10%), Query Frame = 0


Query: 3   MQAFLFITQFTYQNYAI-AMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPT 62
           M+AFL I Q +YQN    A+ KTFT  L MAF IS  FSQCYRK K SV     LPPGP 
Sbjct: 1   MEAFLIIWQLSYQNSPTGAINKTFTVFLFMAF-ISAIFSQCYRKRKHSV---LQLPPGPK 60

Query: 63  PWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHD 122
           PWP VGCLP M    +  ++WIHA+MKQ+DTEIACIRLGNTYVIPVTSP+LA+EFLK HD
Sbjct: 61  PWPLVGCLPSMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVIPVTSPELAVEFLKKHD 120

Query: 123 SVFASRPML-DTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA 182
           SVFASR  + +T  ++TRG LTTA SPLG QWKKMR+IL S+I  PSTLHQML HRT+EA
Sbjct: 121 SVFASRSTVSNTVNVLTRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEA 180

Query: 183 DTLLRYIFSL----TKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEE 242
           DTLLRYIFSL    T ++G AVVN+ SVT HYCGN+IRR+LFNRRYYG G+EDGGP+FEE
Sbjct: 181 DTLLRYIFSLTTTNTNNEGGAVVNVGSVTQHYCGNIIRRMLFNRRYYGKGREDGGPSFEE 240

Query: 243 EEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQW 302
           EEH QALL +L+++NAFS+SDF+PCL+PFD+D H+KI+K  LK + K+DEPI++ERVQQW
Sbjct: 241 EEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQW 300

Query: 303 KYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLN 362
           K GKR+  ED LDI I++K  NGKSLL+IEEIKA+ITELQ+ATIDNPSNAVEW MAELLN
Sbjct: 301 KDGKREGAEDILDILISIKDGNGKSLLTIEEIKAQITELQIATIDNPSNAVEWTMAELLN 360

Query: 363 QPKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVR-----------FIAVLEKESHN 422
           QPKILQKAIEELDR+V R RLVQE DIP+LKYL A VR            I  +      
Sbjct: 361 QPKILQKAIEELDRVVGRGRLVQESDIPKLKYLTASVRESFRLHPFSPFNIPHVSNADII 420

Query: 423 LTGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS 455
           + GYFIPKGS+V+LSR GLGRN +IW+DP+RFDPERHLKD T+  +GLS
Sbjct: 421 VAGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDGTVA-LGLS 464

[Close]

BLAST of Moc06g12140 vs. NCBI nr
Match: XP_038878794.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 604.0 bits (1556), Expect = 1.1e-168
Identity = 299/464 (64.44%), Postives = 374/464 (80.60%), Query Frame = 0


Query: 5   AFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYR-KSKLSVPRHAALPPGPTPW 64
           AFL I+Q  YQN  IA++KTF   + MAF IS+ FS CY  K+K SV R A LPPGP PW
Sbjct: 4   AFLIISQLAYQNSLIAISKTFIVFVFMAF-ISVIFSHCYNLKTKRSVRRKAPLPPGPKPW 63

Query: 65  PFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSV 124
           P VGCLP M T    PHQWIH++MKQ++TEIA IRLGNTY+IPVTSP+LAL+FLK +DS+
Sbjct: 64  PLVGCLPTMLTIKYPPHQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKIYDSI 123

Query: 125 FASRPML-DTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADT 184
           FASR  + +T  I++RG+LTTA S +G+QW+KMR+IL S+ILNP  LH+ML  RT EAD+
Sbjct: 124 FASRSSISNTVDILSRGYLTTAFSSMGDQWRKMRRILASEILNPKMLHRMLGQRTSEADS 183

Query: 185 LLRYIFSLT-KSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQ 244
           LLRYIF+LT K+ G AV+N+RS+T HYCGN+IRR+LFNRRYYG G+EDGGPTFEEE+H Q
Sbjct: 184 LLRYIFTLTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEDHNQ 243

Query: 245 ALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKR 304
           ALL +L+++NAFS+SDF+PCL+PFD+D H+KI+KE L +VRK+DEPI+DERVQ WK  K 
Sbjct: 244 ALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKEALNLVRKYDEPIIDERVQHWKVKKI 303

Query: 305 KEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKIL 364
           + IED LDI I+LK +NG SLLSIEEIKA+I ELQ+ATIDNPSNAVEWAMAEL+NQP++L
Sbjct: 304 RVIEDILDILISLKDDNGNSLLSIEEIKAQIVELQIATIDNPSNAVEWAMAELINQPEVL 363

Query: 365 QKAIEELDRIVERDRLVQEYDIPQLKYLIACVR-----------FIAVLEKESHNLTGYF 424
           +KA+EELD +V R+RLV+E DIP+LKYL AC+R            I  +      + GY 
Sbjct: 364 KKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIVVNGYL 423

Query: 425 IPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS 455
           IPKGS+VLLSRIGLGRN +IW+DP+RF+PERHLKD T+ ++G+S
Sbjct: 424 IPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTVTELGIS 466

[Close]

BLAST of Moc06g12140 vs. NCBI nr
Match: XP_038880015.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 591.7 bits (1524), Expect = 5.5e-165
Identity = 297/464 (64.01%), Postives = 375/464 (80.82%), Query Frame = 0


Query: 5   AFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCY-RKSKLSVPRHAALPPGPTPW 64
           AFLFI+Q  +Q+  IA+TKTF   + MAF  S+ FS  Y R+ KLSV + A LPPGP PW
Sbjct: 4   AFLFISQLAHQHSPIAITKTFIVFVFMAF-TSVLFSHFYNRRRKLSV-QQAPLPPGPKPW 63

Query: 65  PFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSV 124
           P VGCLP + TN +  +QWIH++MKQ++TEIA IRLGNTY+IPVTSP+LAL+FLKT+DS+
Sbjct: 64  PLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSI 123

Query: 125 FASRPML-DTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADT 184
           FASR  + +T  I++RG+LTTA S +G QWKKMR+IL S++LNP++LHQML  RT EAD 
Sbjct: 124 FASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADN 183

Query: 185 LLRYIFSLT-KSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQ 244
           LL YIF LT K+ G AV+N+RS+T HYCGN+IRR+LFNRRYYG G+EDGGPT EEE+H +
Sbjct: 184 LLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHNE 243

Query: 245 ALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKR 304
           ALL +L+++NAFS+SDF+PCL+PFD+D H+KI+K+ L VVRK+DEPI+DERVQQWK  K 
Sbjct: 244 ALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKKI 303

Query: 305 KEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKIL 364
           + IED LDI I+LK ++G SLLSIEEIKA+ITELQ+ATIDNPSNAVEWAMAEL+NQP+IL
Sbjct: 304 RVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEIL 363

Query: 365 QKAIEELDRIVERDRLVQEYDIPQLKYLIACVR-----------FIAVLEKESHNLTGYF 424
           +KA+EELD +V R+RLV+E DIP+LKYL AC+R            I  +      + GY 
Sbjct: 364 KKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGYL 423

Query: 425 IPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS 455
           IPKGS+VLLSRIGLGRN +IW+DP+RF+PERHLKD TM ++G+S
Sbjct: 424 IPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGIS 465

[Close]

BLAST of Moc06g12140 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.1e-126
Identity = 221/430 (51.40%), Postives = 311/430 (72.33%), Query Frame = 0


Query: 28  SLLMAFIISLTFSQCYRK--SKLSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPHQWIHA 87
           SL +AFII +   +   K  +K SVP    LPPGP+PWP VG LPEMW N    H+WIH+
Sbjct: 17  SLSLAFIIFMFLVKFILKTHNKNSVP-VVPLPPGPSPWPIVGSLPEMWRNRP-AHRWIHS 76

Query: 88  IMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITRGFLTTAL 147
           +MK+ +T+IACIRLGN +VIPVTSP++A EFLK +D+VFASRP+   TK ++ G+LTT +
Sbjct: 77  LMKKLNTDIACIRLGNVHVIPVTSPEIAREFLKKNDAVFASRPVTMATKTLSSGYLTTVV 136

Query: 148 SPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFS-LTKSKGAAVVNLRSV 207
            P G+QW+KMR++L ++  NPS +H +L  R  EAD L++++++  + ++  AVVN+R  
Sbjct: 137 GPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEADNLVKFLYNQCSANQNGAVVNVRIA 196

Query: 208 TLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSVSDFIPCLRP 267
              Y G+++R+++FNR Y+G G+EDGGP  EEEEH+ ALL LL Y  AF VSD++P LR 
Sbjct: 197 AQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHVSALLTLLTYAYAFCVSDYLPWLRV 256

Query: 268 FDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYENGKSLLS 327
           FD+D H+K V++ + +V+KH EPIV+ER+Q+W+ GKR E +D LD+FI+LK  NG+ LLS
Sbjct: 257 FDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGKRNEPDDLLDVFISLKDANGQPLLS 316

Query: 328 IEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERDRLVQEYDIP 387
            EEIKA+ TELQ++T+D+P N  EWA+ E+LNQP++L+KA EELDR+V +  LVQE  +P
Sbjct: 317 DEEIKAQTTELQLSTVDSPFNIAEWALTEMLNQPEMLKKAEEELDRVVGKKTLVQESHVP 376

Query: 388 QLKYLIACVRFIAVLEK-----------ESHNLTGYFIPKGSQVLLSRIGLGRNLKIWKD 444
            L Y+ AC + +  L                 + GYFIPKGS V+LSR+ LGRN ++W++
Sbjct: 377 HLPYIRACAKEVMRLHPVGPFNLPHVSIADAEVAGYFIPKGSNVILSRLELGRNPRVWEE 436

[Close]

BLAST of Moc06g12140 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.5e-117
Identity = 216/447 (48.32%), Postives = 302/447 (67.56%), Query Frame = 0


Query: 30  LMAFIISLTFSQCYRKSK-------LSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPHQW 89
           ++AFII L       K K       +S  R+ +LPPGP  WP +G LPE+   N    +W
Sbjct: 7   MLAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRW 66

Query: 90  IHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITRGFLT 149
           IH++MK+ +T+IACIRL NT+VIPVTSP++A E LK  DSVFA+RP+   T+  +RG+LT
Sbjct: 67  IHSLMKELNTDIACIRLANTHVIPVTSPRIAREILKKQDSVFATRPLTMGTEYCSRGYLT 126

Query: 150 TALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFSLT-KSKGAA--VV 209
            A+ P GEQWKKMR+++ S + +  +   ML  RT EAD L+RYI + + K++G A  V+
Sbjct: 127 VAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQKRTEEADNLVRYINNRSVKNRGNAFVVI 186

Query: 210 NLRSVTLHYCGNVIRRVLFNRRYYGAGQEDG-GPTFEEEEHIQALLALLKYINAFSVSDF 269
           +LR     Y GNV R+++F  R++G G EDG GP  EE EH+++L  +L ++ AF++SD+
Sbjct: 187 DLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDY 246

Query: 270 IPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYEN 329
           +P LR  D++ H+K+V   ++ V K+++P VDER+ QW+ GK KE +DFLD+FI  K  +
Sbjct: 247 VPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIIAKDTD 306

Query: 330 GKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERDRLV 389
           GK  LS EEIKA++TEL +AT+DNPSNA EW MAE++N+P I+QKA+EE+DR+V +DRLV
Sbjct: 307 GKPTLSDEEIKAQVTELMLATVDNPSNAAEWGMAEMINEPSIMQKAVEEIDRVVGKDRLV 366

Query: 390 QEYDIPQLKYLIACVRFIAVLEKES-----HNLT------GYFIPKGSQVLLSRIGLGRN 449
            E D+P L Y+ ACV+    L   +     H  T      GYFIPKGS VL+SR+G+GRN
Sbjct: 367 IESDLPNLNYVKACVKEAFRLHPVAPFNLPHMSTTDTVVDGYFIPKGSHVLISRMGIGRN 426

Query: 450 LKIWKDPIRFDPERHLKDKTMGDMGLS 455
             +W  P +FDPERHL   T  D+  S
Sbjct: 427 PSVWDKPHKFDPERHLSTNTCVDLNES 453

[Close]

BLAST of Moc06g12140 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 5.5e-115
Identity = 206/441 (46.71%), Postives = 292/441 (66.21%), Query Frame = 0


Query: 19  IAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPTPWPFVGCLPEMWTNNTL 78
           +++   F ++ L+  I S    +  +   ++  +   LPPGP PWP VG LPEM  N   
Sbjct: 8   LSLCHEFPWTFLLVVIFSFMIFKVTKTHLVNKSKKYKLPPGPKPWPIVGNLPEMLANRPA 67

Query: 79  PHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITR 138
              WIH +MK+ +TEIACIRL NT VIPVT P +A EFLK HD+ FASRP + +T I + 
Sbjct: 68  T-IWIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMSTDIASD 127

Query: 139 GFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFS---LTKSKG 198
           GF+TT L P GEQWKKM+++L + +L+P     +L  R  EAD L+ YI++      + G
Sbjct: 128 GFITTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDG 187

Query: 199 AAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSV 258
             +VN+R    HY GNV R+++FN RY+G   EDGGP FEE EHI A   +LKY+ AFS+
Sbjct: 188 PGLVNIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSI 247

Query: 259 SDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALK 318
           SDF+P LR  D+D H+  + + ++++RK+ +PI+D+R++QW  G +   ED LD+ I LK
Sbjct: 248 SDFVPFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLKTVEEDLLDVLIKLK 307

Query: 319 YENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERD 378
             N K LL+++E+KA+I EL +  +DNPSNA EWA+AE++NQP++L++A EELD +V ++
Sbjct: 308 DANNKPLLTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNVVGKE 367

Query: 379 RLVQEYDIPQLKYLIACVRFIAVLEKESH----------NLTG-YFIPKGSQVLLSRIGL 438
           RLVQE DIP+L+++ AC R    L    +           + G Y  PKG+QVLLSR+ L
Sbjct: 368 RLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVAL 427

Query: 439 GRNLKIWKDPIRFDPERHLKD 446
           GRN K W DP++F+PERHLK+
Sbjct: 428 GRNPKFWTDPLKFNPERHLKE 447

[Close]

BLAST of Moc06g12140 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.6e-114
Identity = 214/447 (47.87%), Postives = 294/447 (65.77%), Query Frame = 0


Query: 14  YQNYAIAMTKTFTFSLLMAFII-SLTFSQCYRKSKLSVPRHAALPPGPTPWPFVGCLPEM 73
           + ++ +  + TF   ++  FII  +T S    KSK        LPPGP PWP VG LPEM
Sbjct: 7   FLSFCLEFSWTFLLVVIFFFIIFKVTKSHSVNKSK-----KYKLPPGPKPWPIVGNLPEM 66

Query: 74  WTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDT 133
             N      WIH +MK+ +TEIACIRL NT VIPVT P +A EFLK HD+ FASRP + +
Sbjct: 67  LANRPAT-IWIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMS 126

Query: 134 TKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFS--- 193
           T I + GFLTT L P GEQWKKM+++L + +L+P     +L  R  EAD L+ YI++   
Sbjct: 127 TDIASDGFLTTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCC 186

Query: 194 LTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQALLALLKY 253
              + G  +VN+R    HY GNV R+++FN RY+G   EDGGP FEE EHI A   +LKY
Sbjct: 187 KDVNDGPGLVNIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKY 246

Query: 254 INAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLD 313
           + AFS+SDFIP LR  D+D H+  + + + +++K+ +PI+ +R++QW  G +   ED LD
Sbjct: 247 VYAFSISDFIPFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLKTVEEDLLD 306

Query: 314 IFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELD 373
           + I LK  + K LL+++EIKA+ITEL +  +DNPSNA EWA+AE+LNQP++L++A EELD
Sbjct: 307 VLIKLKDASNKPLLTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELD 366

Query: 374 RIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESH----------NLTG-YFIPKGSQVL 433
            +V ++RLVQE DIP+L+++ AC R    L    +           + G Y  PKG+QVL
Sbjct: 367 NVVGKERLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVL 426

Query: 434 LSRIGLGRNLKIWKDPIRFDPERHLKD 446
           LSR+ LGRN K W DP++F+PERHLK+
Sbjct: 427 LSRVALGRNPKFWTDPLKFNPERHLKE 447

[Close]

BLAST of Moc06g12140 vs. ExPASy Swiss-Prot
Match: Q9M7B8 (Valine N-monooxygenase 1 OS=Manihot esculenta OX=3983 GN=CYP79D1 PE=1 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.1e-110
Identity = 204/430 (47.44%), Postives = 283/430 (65.81%), Query Frame = 0


Query: 27  FSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPHQWIHAI 86
           F  +++ I+ L  S   ++    +P    LPPGPTPWP +G +PEM        +WIH +
Sbjct: 34  FISIVSTIVKLQKSAANKEGSKKLP----LPPGPTPWPLIGNIPEM-IRYRPTFRWIHQL 93

Query: 87  MKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITRGFLTTALS 146
           MK  +T+I  IR G T  +P++ P LA E LK +D++F++RP   + K ++ G+LTT + 
Sbjct: 94  MKDMNTDICLIRFGRTNFVPISCPVLAREILKKNDAIFSNRPKTLSAKSMSGGYLTTIVV 153

Query: 147 PLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFSLTKSKGAAVVNLRSVTL 206
           P  +QWKKMRKILTS+I++P+    +   R  EAD L+ YI +  + K    VNLR+ T 
Sbjct: 154 PYNDQWKKMRKILTSEIISPARHKWLHDKRAEEADNLVFYIHN--QFKANKNVNLRTATR 213

Query: 207 HYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSVSDFIPCLRPFD 266
           HY GNVIR+++F++RY+G G  DGGP  EE EHI A+   LKY+  F +SDF+P L   D
Sbjct: 214 HYGGNVIRKMVFSKRYFGKGMPDGGPGPEEIEHIDAVFTALKYLYGFCISDFLPFLLGLD 273

Query: 267 VDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYENGKSLLSIE 326
           +D  +K V +  K +R +  P++DER+QQWK G+RKE+ED LD+FI LK  +G  LL+ +
Sbjct: 274 LDGQEKFVLDANKTIRDYQNPLIDERIQQWKSGERKEMEDLLDVFITLKDSDGNPLLTPD 333

Query: 327 EIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERDRLVQEYDIPQL 386
           EIK +I E+ +AT+DNPSNA+EWAM E+LNQP+IL+KA EELDR+V +DRLVQE DIP L
Sbjct: 334 EIKNQIAEIMIATVDNPSNAIEWAMGEMLNQPEILKKATEELDRVVGKDRLVQESDIPNL 393

Query: 387 KYLIACVRFIAVLEKESH-----------NLTGYFIPKGSQVLLSRIGLGRNLKIWKDPI 446
            Y+ AC R    L   +H            +  YFIPKGS  +LSR GLGRN K W DP+
Sbjct: 394 DYVKACAREAFRLHPVAHFNVPHVAMEDTVIGDYFIPKGSWAVLSRYGLGRNPKTWSDPL 453

[Close]

BLAST of Moc06g12140 vs. ExPASy TrEMBL
Match: A0A6J1DLV4 (phenylalanine N-monooxygenase-like OS=Momordica charantia OX=3673 GN=LOC111021650 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.8e-231
Identity = 406/407 (99.75%), Postives = 407/407 (100.00%), Query Frame = 0


Query: 1   MAMQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGP 60
           MAMQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGP
Sbjct: 1   MAMQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGP 60

Query: 61  TPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTH 120
           TPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTH
Sbjct: 61  TPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTH 120

Query: 121 DSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA 180
           DSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA
Sbjct: 121 DSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA 180

Query: 181 DTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHI 240
           DTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHI
Sbjct: 181 DTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHI 240

Query: 241 QALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGK 300
           QALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGK
Sbjct: 241 QALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGK 300

Query: 301 RKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKI 360
           RKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKI
Sbjct: 301 RKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKI 360

Query: 361 LQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESHNLT 408
           LQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESHNL+
Sbjct: 361 LQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESHNLS 407

[Close]

BLAST of Moc06g12140 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 7.8e-173
Identity = 314/468 (67.09%), Postives = 375/468 (80.13%), Query Frame = 0


Query: 3   MQAFLFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPTP 62
           M+A LFI+Q TYQN   A+TKTFT  + MAF IS  FSQCYRK K SV     LPPGP P
Sbjct: 1   MEALLFISQLTYQNSPTAITKTFTLFVFMAF-ISAIFSQCYRKRKHSV---LQLPPGPNP 60

Query: 63  WPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDS 122
           WP VGCLP M    +  ++WIHA+MKQ+DTEIACIRLGNTYV+PVTSP+LA+EFLK HDS
Sbjct: 61  WPLVGCLPAMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVMPVTSPELAVEFLKRHDS 120

Query: 123 VFASRPML-DTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEAD 182
           VFASR  + +T  +++RG LTTA SPLG QWKKMR+IL S+I  PSTLHQML HRT+EAD
Sbjct: 121 VFASRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEAD 180

Query: 183 TLLRYIFSLTK----SKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEE 242
           TLLRYIFSLT     +K  AVVN+RSVT HYCGN+IRR+LFN+RYYG G+EDGGP+FEEE
Sbjct: 181 TLLRYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSFEEE 240

Query: 243 EHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWK 302
           EH QALL +L+++NAFS+SDF+PCL+PFD+D H+KI+K  LK + K+DEPI++ERVQQWK
Sbjct: 241 EHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQWK 300

Query: 303 YGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQ 362
            GKR+  ED LDI I+ K  NGKSLL+I+EIKA+ITELQ+ATIDNPSNAVEW MAELLNQ
Sbjct: 301 DGKREGAEDILDILISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAELLNQ 360

Query: 363 PKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVR-----------FIAVLEKESHNL 422
           PKILQKAIEELDR+V R+RLVQE DIP+LKYL AC R            I  +      +
Sbjct: 361 PKILQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVV 420

Query: 423 TGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS 455
            GYFIPKGS+V+LSR GLGRN +IW+DP+RFDPERHLKD+T+ ++GLS
Sbjct: 421 AGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETV-ELGLS 463

[Close]

BLAST of Moc06g12140 vs. ExPASy TrEMBL
Match: A0A6J1D6G3 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 3.6e-170
Identity = 316/469 (67.38%), Postives = 371/469 (79.10%), Query Frame = 0


Query: 3   MQAFLFITQFTYQNYAI-AMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPT 62
           M+AFL I Q +YQN    A+ KTFT  L MAF IS  FSQCYRK K SV     LPPGP 
Sbjct: 1   MEAFLIIWQLSYQNSPTGAINKTFTVFLFMAF-ISAIFSQCYRKRKHSV---LQLPPGPK 60

Query: 63  PWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHD 122
           PWP VGCLP M    +  ++WIHA+MKQ+DTEIACIRLGNTYVIPVTSP+LA+EFLK HD
Sbjct: 61  PWPLVGCLPSMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVIPVTSPELAVEFLKKHD 120

Query: 123 SVFASRPML-DTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEA 182
           SVFASR  + +T  ++TRG LTTA SPLG QWKKMR+IL S+I  PSTLHQML HRT+EA
Sbjct: 121 SVFASRSTVSNTVNVLTRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEA 180

Query: 183 DTLLRYIFSL----TKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEE 242
           DTLLRYIFSL    T ++G AVVN+ SVT HYCGN+IRR+LFNRRYYG G+EDGGP+FEE
Sbjct: 181 DTLLRYIFSLTTTNTNNEGGAVVNVGSVTQHYCGNIIRRMLFNRRYYGKGREDGGPSFEE 240

Query: 243 EEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQW 302
           EEH QALL +L+++NAFS+SDF+PCL+PFD+D H+KI+K  LK + K+DEPI++ERVQQW
Sbjct: 241 EEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQW 300

Query: 303 KYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLN 362
           K GKR+  ED LDI I++K  NGKSLL+IEEIKA+ITELQ+ATIDNPSNAVEW MAELLN
Sbjct: 301 KDGKREGAEDILDILISIKDGNGKSLLTIEEIKAQITELQIATIDNPSNAVEWTMAELLN 360

Query: 363 QPKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVR-----------FIAVLEKESHN 422
           QPKILQKAIEELDR+V R RLVQE DIP+LKYL A VR            I  +      
Sbjct: 361 QPKILQKAIEELDRVVGRGRLVQESDIPKLKYLTASVRESFRLHPFSPFNIPHVSNADII 420

Query: 423 LTGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS 455
           + GYFIPKGS+V+LSR GLGRN +IW+DP+RFDPERHLKD T+  +GLS
Sbjct: 421 VAGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDGTVA-LGLS 464

[Close]

BLAST of Moc06g12140 vs. ExPASy TrEMBL
Match: A0A1S3BQ37 (isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 6.0e-165
Identity = 293/461 (63.56%), Postives = 369/461 (80.04%), Query Frame = 0


Query: 7   LFITQFTYQNYAIAMTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPTPWPFV 66
           +FITQ  +QN  IA++K F   L MAF  S+ FS+CY + K    R A LPPGP PWP V
Sbjct: 7   IFITQLPHQNSPIAISKAFIVFLFMAF-ASVMFSRCYNRRKKYSVRKALLPPGPKPWPIV 66

Query: 67  GCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFAS 126
           GCLP M TN +  +QWIH++MKQ++TEIACIRLGNTYVIPVTSP+LALEFL+THDSVF+S
Sbjct: 67  GCLPTMLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRTHDSVFSS 126

Query: 127 RPML-DTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLR 186
           R  + +T  I++RG+LTTA S +G+QWKKMR+IL S+IL+P+ LH+ML  R  EADTLL 
Sbjct: 127 RSSISNTVDILSRGYLTTAFSSMGDQWKKMRRILASEILSPTMLHRMLEQRIAEADTLLH 186

Query: 187 YIFSLT-KSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQALL 246
           YIFS+T ++ G AV+N+RS+T HYCGN+IR +LFNRRYYG G+EDGGPT EEEEH QALL
Sbjct: 187 YIFSITSENGGGAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTSEEEEHNQALL 246

Query: 247 ALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEI 306
            +L++INAFS+SDF+PCL+PFD+D H+KI+K  L  VRK+DEPI+DERVQQWK  K+ E+
Sbjct: 247 TILRHINAFSISDFMPCLKPFDLDGHEKIMKNALNAVRKYDEPIIDERVQQWKKDKKIEV 306

Query: 307 EDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKA 366
           +D LDI I+ K +NG SLLSI+EIKA+ITELQ+ATIDNPSNAVEWAMAEL+NQP++L+K 
Sbjct: 307 KDILDILISRKDDNGNSLLSIDEIKAQITELQIATIDNPSNAVEWAMAELINQPEVLKKV 366

Query: 367 IEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEKESH-----------NLTGYFIPK 426
           ++ELD +V ++RLVQEYDIP+LKYL AC+R    L   S             + GY IPK
Sbjct: 367 VDELDNVVGKERLVQEYDIPKLKYLTACLRESFRLHPFSPFNVPHVPTFDIVVNGYLIPK 426

Query: 427 GSQVLLSRIGLGRNLKIWKDPIRFDPERHLKDKTMGDMGLS 455
           GS+VLLSR+GLGRN +IWKDPI+F+PERHLKD T+ ++GLS
Sbjct: 427 GSEVLLSRLGLGRNPRIWKDPIKFNPERHLKDNTV-ELGLS 465

[Close]

BLAST of Moc06g12140 vs. ExPASy TrEMBL
Match: A0A6J1DJC5 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021624 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 3.3e-163
Identity = 292/429 (68.07%), Postives = 348/429 (81.12%), Query Frame = 0


Query: 39  FSQCYRKSKLSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIR 98
           FSQCY K K SV +   LPPGP PWP VGCLP +   N+  ++WIHA+MKQ+DTEIACIR
Sbjct: 2   FSQCYCKRKNSVLK---LPPGPKPWPLVGCLPSILATNSPKYKWIHAVMKQFDTEIACIR 61

Query: 99  LGNTYVIPVTSPQLALEFLKTHDSVFASRPML-DTTKIITRGFLTTALSPLGEQWKKMRK 158
           LGNTYVIPVTSP+LA+EFLKTHDSVFASR  + +T  + TRGFLTTA SP G+QWKKMR+
Sbjct: 62  LGNTYVIPVTSPELAVEFLKTHDSVFASRSTISNTVNLFTRGFLTTAFSPSGDQWKKMRR 121

Query: 159 ILTSQILNPST-LHQMLAHRTHEADTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRV 218
           ILTS+IL+PST LHQ+L HRT+EA  LLRYIF LT     AVVNLRS+T HYCGNVIRR 
Sbjct: 122 ILTSEILSPSTDLHQILGHRTNEAHILLRYIFKLTAGGAGAVVNLRSITQHYCGNVIRRS 181

Query: 219 LFNRRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKE 278
           LFN RYYG G EDGGP+FEEEEH QALL +LK+INAFS+SDF+PCL+PFD+D H KI+K+
Sbjct: 182 LFNTRYYGKGSEDGGPSFEEEEHNQALLTILKHINAFSISDFMPCLKPFDLDGHGKIMKQ 241

Query: 279 GLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQ 338
            L+V+RK+DEPI++ERVQQWK GKR+ +ED LDI I+LK E+G+SLLSI+EIKA+ITELQ
Sbjct: 242 ALEVLRKYDEPIINERVQQWKDGKREGVEDILDILISLKDESGESLLSIDEIKAQITELQ 301

Query: 339 MATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVR-- 398
           +ATIDNPSN VEW MAELLNQPKILQKA+EELDR+V RDRLVQE DIP+LKYL AC R  
Sbjct: 302 IATIDNPSNTVEWTMAELLNQPKILQKAVEELDRVVGRDRLVQESDIPKLKYLTACARES 361

Query: 399 ---------FIAVLEKESHNLTGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKD 455
                     I  +      + G+FIPKGS+V+LSR GLGRN +IW++P RFDPERHL D
Sbjct: 362 LRLHPFSPFNIPHVSNSDVIVAGFFIPKGSEVILSRPGLGRNPRIWENPNRFDPERHLTD 421

[Close]

BLAST of Moc06g12140 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 422.9 bits (1086), Expect = 3.2e-118
Identity = 216/447 (48.32%), Postives = 302/447 (67.56%), Query Frame = 0


Query: 30  LMAFIISLTFSQCYRKSK-------LSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPHQW 89
           ++AFII L       K K       +S  R+ +LPPGP  WP +G LPE+   N    +W
Sbjct: 1   MLAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRW 60

Query: 90  IHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITRGFLT 149
           IH++MK+ +T+IACIRL NT+VIPVTSP++A E LK  DSVFA+RP+   T+  +RG+LT
Sbjct: 61  IHSLMKELNTDIACIRLANTHVIPVTSPRIAREILKKQDSVFATRPLTMGTEYCSRGYLT 120

Query: 150 TALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFSLT-KSKGAA--VV 209
            A+ P GEQWKKMR+++ S + +  +   ML  RT EAD L+RYI + + K++G A  V+
Sbjct: 121 VAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQKRTEEADNLVRYINNRSVKNRGNAFVVI 180

Query: 210 NLRSVTLHYCGNVIRRVLFNRRYYGAGQEDG-GPTFEEEEHIQALLALLKYINAFSVSDF 269
           +LR     Y GNV R+++F  R++G G EDG GP  EE EH+++L  +L ++ AF++SD+
Sbjct: 181 DLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDY 240

Query: 270 IPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYEN 329
           +P LR  D++ H+K+V   ++ V K+++P VDER+ QW+ GK KE +DFLD+FI  K  +
Sbjct: 241 VPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIIAKDTD 300

Query: 330 GKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERDRLV 389
           GK  LS EEIKA++TEL +AT+DNPSNA EW MAE++N+P I+QKA+EE+DR+V +DRLV
Sbjct: 301 GKPTLSDEEIKAQVTELMLATVDNPSNAAEWGMAEMINEPSIMQKAVEEIDRVVGKDRLV 360

Query: 390 QEYDIPQLKYLIACVRFIAVLEKES-----HNLT------GYFIPKGSQVLLSRIGLGRN 449
            E D+P L Y+ ACV+    L   +     H  T      GYFIPKGS VL+SR+G+GRN
Sbjct: 361 IESDLPNLNYVKACVKEAFRLHPVAPFNLPHMSTTDTVVDGYFIPKGSHVLISRMGIGRN 420

Query: 450 LKIWKDPIRFDPERHLKDKTMGDMGLS 455
             +W  P +FDPERHL   T  D+  S
Sbjct: 421 PSVWDKPHKFDPERHLSTNTCVDLNES 447

[Close]

BLAST of Moc06g12140 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-107
Identity = 192/402 (47.76%), Postives = 277/402 (68.91%), Query Frame = 0


Query: 56  LPPGPTPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALE 115
           LPPGPT +P VG +P M  N  +  +W+H++MK+ +TEIAC+RLGNT+VIPVT P++A E
Sbjct: 58  LPPGPTGFPIVGMIPAMLKNRPV-FRWLHSLMKELNTEIACVRLGNTHVIPVTCPKIARE 117

Query: 116 FLKTHDSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAH 175
             K  D++FASRP+    KI++ G+ T  ++P GEQ+KKMRK++ ++I+ P+    +  +
Sbjct: 118 IFKQQDALFASRPLTYAQKILSNGYKTCVITPFGEQFKKMRKVIMTEIVCPARHRWLHDN 177

Query: 176 RTHEADTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQE-DGGPTF 235
           R  E D L  +++++ K+  +  V+LR VT HYCGN I+R++F  R +    E DGGPT 
Sbjct: 178 RAEETDHLTAWLYNMVKN--SEPVDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTL 237

Query: 236 EEEEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQ 295
           E+ EH+ A+   L +  AF +SD++P L   D++ H+KI++E   ++ K+ +PI+DER++
Sbjct: 238 EDIEHMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIK 297

Query: 296 QWKYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAEL 355
            W+ GKR +IEDFLDIFI++K E G+ LL+ +EIK  I EL MA  DNPSNAVEWA+AE+
Sbjct: 298 MWREGKRTQIEDFLDIFISIKDEAGQPLLTADEIKPTIKELVMAAPDNPSNAVEWAIAEM 357

Query: 356 LNQPKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEK-----------ES 415
           +N+P+IL KA+EE+DR+V ++R VQE DIP+L Y+ A +R    L               
Sbjct: 358 INKPEILHKAMEEIDRVVGKERFVQESDIPKLNYVKAIIREAFRLHPVAAFNLPHVALSD 417

Query: 416 HNLTGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKD 446
             + GY IPKGSQVLLSR GLGRN K+W DP+ F PERHL +
Sbjct: 418 TTVAGYHIPKGSQVLLSRYGLGRNPKVWSDPLSFKPERHLNE 456

[Close]

BLAST of Moc06g12140 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 387.5 bits (994), Expect = 1.5e-107
Identity = 189/402 (47.01%), Postives = 278/402 (69.15%), Query Frame = 0


Query: 56  LPPGPTPWPFVGCLPEMWTNNTLPHQWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALE 115
           LPPGPT WP +G +P M  +  +  +W+H+IMKQ +TEIAC++LGNT+VI VT P++A E
Sbjct: 56  LPPGPTGWPIIGMIPTMLKSRPV-FRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIARE 115

Query: 116 FLKTHDSVFASRPMLDTTKIITRGFLTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAH 175
            LK  D++FASRP+    KI++ G+ T  ++P G+Q+KKMRK++ ++++ P+    +   
Sbjct: 116 ILKQQDALFASRPLTYAQKILSNGYKTCVITPFGDQFKKMRKVVMTELVCPARHRWLHQK 175

Query: 176 RTHEADTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFNRRYYGAG-QEDGGPTF 235
           R+ E D L  +++++ K+ G+  V+ R +T HYCGN I++++F  R +      DGGPT 
Sbjct: 176 RSEENDHLTAWVYNMVKNSGS--VDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGPTV 235

Query: 236 EEEEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQ 295
           E+ EH++A+   L +  AF +SD++P L   D++ H+KI++E   ++ K+ +PI+DER++
Sbjct: 236 EDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIK 295

Query: 296 QWKYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAEL 355
            W+ GKR +IEDFLDIFI++K E G  LL+ +EIK  I EL MA  DNPSNAVEWAMAE+
Sbjct: 296 MWREGKRTQIEDFLDIFISIKDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVEWAMAEM 355

Query: 356 LNQPKILQKAIEELDRIVERDRLVQEYDIPQLKYLIACVRFIAVLEK-----------ES 415
           +N+P+IL+KA+EE+DR+V ++RLVQE DIP+L Y+ A +R    L               
Sbjct: 356 VNKPEILRKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLHPVAAFNLPHVALSD 415

Query: 416 HNLTGYFIPKGSQVLLSRIGLGRNLKIWKDPIRFDPERHLKD 446
             + GY IPKGSQVLLSR GLGRN K+W DP+ F PERHL +
Sbjct: 416 TTVAGYHIPKGSQVLLSRYGLGRNPKVWADPLCFKPERHLNE 454

[Close]

BLAST of Moc06g12140 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 386.7 bits (992), Expect = 2.5e-107
Identity = 199/437 (45.54%), Postives = 284/437 (64.99%), Query Frame = 0


Query: 30  LMAFIISLTFSQCYRKSK--------LSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPHQ 89
           ++AFI+ L       K K         S  R+ +LPPGP  WP VG LPE+   N    +
Sbjct: 8   MLAFILGLLLLSLTMKRKETKKTMLMTSHTRNLSLPPGPKSWPLVGNLPEILGRNKPVFR 67

Query: 90  WIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITRGFL 149
           WIH++M++ +T+IACIRL NT+VIPVTSP++A E LK  DSVFA RP+   T+  +RG+L
Sbjct: 68  WIHSLMEELNTDIACIRLANTHVIPVTSPRIAREILKKQDSVFAIRPLTMGTEYCSRGYL 127

Query: 150 TTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFSLT-----KSKGA 209
           T A+   GEQWKKMR+++ S + +  +   ML  RT EAD L+RYI +          G 
Sbjct: 128 TIAVQSQGEQWKKMRRVVASHVTSKKSFKLMLEKRTEEADNLVRYINNRCVKNRGNGNGL 187

Query: 210 AVVNLRSVTLHYCGNVIRRVLFNRRYYGAGQED-GGPTFEEEEHIQALLALLKYINAFSV 269
           AV++LR V   Y GNV R+++F  R++G G ED  GP  EE EH+++L  +L ++ AF++
Sbjct: 188 AVIDLRFVVRQYSGNVARKMMFGIRHFGKGSEDESGPGSEEIEHVESLFTVLTHLYAFAL 247

Query: 270 SDFIPCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALK 329
           SD++P LR  D++ H+K+V + ++ V K+++P VDER+ QW+ GK KE +DFLD+FI  K
Sbjct: 248 SDYVPWLRFLDLEGHEKVVADAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIMAK 307

Query: 330 YENGKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERD 389
             NGK  LS EEIKA++TEL +AT+DNPSNA EWAMAE++N+P I+QKA+EE+DR   R+
Sbjct: 308 DTNGKPTLSEEEIKAQVTELMLATVDNPSNAAEWAMAEMINEPSIMQKAVEEIDRSNLRE 367

Query: 390 RLVQE---------YDIPQLKYLIACVRFIAVLEKESHNLTGYFIPKGSQVLLSRIGLGR 444
            + +E         +++P +    A V              GYFIPKGS +L+SR+G+GR
Sbjct: 368 SVCEETFRLHPVAPFNLPHMSTADAVV-------------DGYFIPKGSHMLISRMGIGR 427

[Close]

BLAST of Moc06g12140 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 319.3 bits (817), Expect = 4.9e-87
Identity = 173/447 (38.70%), Postives = 272/447 (60.85%), Query Frame = 0


Query: 21  MTKTFTFSLLMAFIISLTFSQCYRKSKLSVPRHAALPPGPTPWPFVGCLPEMWTNNTLPH 80
           +T   +F+L++   I+L  +   R S+   P+   LPPGP  WP VG + +M  N    H
Sbjct: 4   ITNPSSFTLVL-ISITLVLALARRFSRFMKPK-GQLPPGPRGWPIVGNMLQMIINRP-AH 63

Query: 81  QWIHAIMKQYDTEIACIRLGNTYVIPVTSPQLALEFLKTHDSVFASRPMLDTTKIITRGF 140
            WIH +M++  T+IAC R    +VI VTS ++A E L+  D V A R     + +I+ G+
Sbjct: 64  LWIHRVMEELQTDIACYRFARFHVITVTSSKIAREVLREKDEVLADRSESYASHLISHGY 123

Query: 141 LTTALSPLGEQWKKMRKILTSQILNPSTLHQMLAHRTHEADTLLRYIFSLTK-SKGAAVV 200
              + S  GE WK ++K++T+++++P+TL + L +R  EAD ++ Y+++L +       +
Sbjct: 124 KNISFSSYGENWKLVKKVMTTKLMSPTTLSKTLGYRNIEADNIVTYVYNLCQLGSVRKPI 183

Query: 201 NLRSVTLHYCGNVIRRVLFNRRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSVSDFI 260
           N+R   L YC  V+ R++F +R++    E+GG   +E+EH+ A+   L    +F+++++I
Sbjct: 184 NVRDTILTYCHAVMMRMMFGQRHFDEVVENGGLGPKEKEHMDAIYLALDCFFSFNLTNYI 243

Query: 261 PCLRPFDVDKHQKIVKEGLKVVRKHDEPIVDERVQQW-KYGKRKEIEDFLDIFIALKYEN 320
           P LR ++VDK +  V+E + ++   ++PI+ ER+  W K G ++  ED+LDI I LK + 
Sbjct: 244 PFLRGWNVDKAETEVREAVHIINICNDPIIQERIHLWRKKGGKQMEEDWLDILITLKDDQ 303

Query: 321 GKSLLSIEEIKAEITELQMATIDNPSNAVEWAMAELLNQPKILQKAIEELDRIVERDRLV 380
           G  L + +EI+A+  E+ +ATIDN  N VEW +AE+LN P+IL+KA  ELD IV +DRLV
Sbjct: 304 GMHLFTFDEIRAQCKEINLATIDNTMNNVEWTIAEMLNHPEILEKATNELDIIVGKDRLV 363

Query: 381 QEYDIPQLKYLIACVRFIAVLE-----------KESHNLTGYFIPKGSQVLLSRIGLGRN 440
           QE DI QL Y+ AC +    L            +E   L GYF+PKGSQ+L+SR+GLGRN
Sbjct: 364 QESDISQLNYIKACSKESFRLHPANVFMPHHVAREDTTLAGYFVPKGSQILVSRLGLGRN 423

Query: 441 LKIWKDPIRFDPERHLKDKTMGDMGLS 455
            KIW +P  F PER+L       +G++
Sbjct: 424 PKIWDEPNAFKPERYLDGHVEKSLGVT 447

The following BLAST results are available for this feature:

BLAST of Moc06g12140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154369.1	9.9e-231	99.75	phenylalanine N-monooxygenase-like [Momordica charantia]	[more]
XP_022148957.1	1.6e-172	67.09	isoleucine N-monooxygenase 2-like [Momordica charantia]	[more]
XP_022148952.1	7.5e-170	67.38	isoleucine N-monooxygenase 2-like [Momordica charantia]	[more]
XP_038878794.1	1.1e-168	64.44	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_038880015.1	5.5e-165	64.01	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]

BLAST of Moc06g12140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q605	1.1e-126	51.40	Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV...	[more]
Q9FLC8	4.5e-117	48.32	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV...	[more]
Q6J541	5.5e-115	46.71	Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1	[more]
Q6J540	1.6e-114	47.87	Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1	[more]
Q9M7B8	1.1e-110	47.44	Valine N-monooxygenase 1 OS=Manihot esculenta OX=3983 GN=CYP79D1 PE=1 SV=1	[more]

BLAST of Moc06g12140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLV4	4.8e-231	99.75	phenylalanine N-monooxygenase-like OS=Momordica charantia OX=3673 GN=LOC11102165...	[more]
A0A6J1D6G9	7.8e-173	67.09	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498...	[more]
A0A6J1D6G3	3.6e-170	67.38	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495...	[more]
A0A1S3BQ37	6.0e-165	63.56	isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 S...	[more]
A0A6J1DJC5	3.3e-163	68.07	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021624...	[more]

BLAST of Moc06g12140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05260.1	3.2e-118	48.32	cytochrome p450 79a2	[more]
AT2G22330.1	1.1e-107	47.76	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT4G39950.1	1.5e-107	47.01	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT5G35917.1	2.5e-107	45.54	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT1G58260.1	4.9e-87	38.70	cytochrome p450 79c2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 347..373 score: 30.62 coord: 420..444 score: 29.24 coord: 112..133 score: 26.26 coord: 208..226 score: 26.53
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 57..447 e-value: 1.1E-56 score: 192.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 41..451 e-value: 2.9E-71 score: 242.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 57..447
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 30..448
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 30..448

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g12140.1	Moc06g12140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen