Homology
BLAST of Moc06g10510 vs. NCBI nr
Match:
XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])
HSP 1 Score: 994.2 bits (2569), Expect = 4.7e-286
Identity = 464/583 (79.59%), Postives = 505/583 (86.62%), Query Frame = 0
Query: 3 IFAEPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGIN 62
+F +PFL IFF+L FG LSE KIRH++WEVEYMFWSPDCVEN+VMGI+
Sbjct: 9 LFPKPFL-----IFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGID 68
Query: 63 HQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGET 122
QFPGPTIRAN GDTVVVELTNKLHTEGVVIHWHGI+Q GTPWADGTASISQCAINPGET
Sbjct: 69 GQFPGPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAINPGET 128
Query: 123 FVYRFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQS 182
F YRFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG+ EPFHYDEEINLLLSDWWHQS
Sbjct: 129 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQS 188
Query: 183 VHRQEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRV 242
VH+QEVGLSS P RWIGEPQ++LINGRGQF+CSIAAKYNN L+QC LKG EQCAPFIL V
Sbjct: 189 VHKQEVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPFILHV 248
Query: 243 LPNKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINT 302
LPNK YRIRIASTTALASLNFAIGNH+LLVVEADGNY+QPFLT+DIDIYSGE+YSVLI T
Sbjct: 249 LPNKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSVLITT 308
Query: 303 DQNPSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTT 362
DQ SENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT
Sbjct: 309 DQPSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSKNFTF 368
Query: 363 RITAAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAF 422
RI AAMGSP+PP ++RRI LLNTQNR+NG VKWAINNVSL LPPTPYL ++KF+LN AF
Sbjct: 369 RIFAAMGSPKPPMRYNRRIFLLNTQNRMNGFVKWAINNVSLALPPTPYLAAMKFKLNTAF 428
Query: 423 DQKPPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWH 482
DQ PPPETFP DYDI+ PP N NT T +GVYR ++G VDVILQN+N LN+ SEIHPWH
Sbjct: 429 DQNPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSEIHPWH 488
Query: 483 LHGHDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHC 542
LHGHDFWVLGYGEGKFS DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHC
Sbjct: 489 LHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHC 548
Query: 543 HIEPHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
HIEPHLHMGMGVVFAEGVHKVG +P + L CG K L+ + P
Sbjct: 549 HIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGAAKALINNNP 586
BLAST of Moc06g10510 vs. NCBI nr
Match:
NP_001284441.1 (L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis melo])
HSP 1 Score: 981.1 bits (2535), Expect = 4.1e-282
Identity = 459/580 (79.14%), Postives = 499/580 (86.03%), Query Frame = 0
Query: 6 EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
+PF ++FFVL FG LSE KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7 KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66
Query: 66 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67 PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126
Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186
Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246
Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306
Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366
Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426
Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486
Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
HDFWVLGYGEGKFS D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546
Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGV+FAEGVHKVG +P + CG T ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGST--AMIKNYP 584
BLAST of Moc06g10510 vs. NCBI nr
Match:
KAA0055636.1 (L-ascorbate oxidase [Cucumis melo var. makuwa])
HSP 1 Score: 979.2 bits (2530), Expect = 1.6e-281
Identity = 458/580 (78.97%), Postives = 498/580 (85.86%), Query Frame = 0
Query: 6 EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
+PF ++FFVL FG LSE KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7 KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66
Query: 66 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67 PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126
Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186
Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246
Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
K YRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306
Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366
Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426
Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486
Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
HDFWVLGYGEGKFS D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546
Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGV+FAEGVHKVG +P + CG T ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGST--AMIKNYP 584
BLAST of Moc06g10510 vs. NCBI nr
Match:
XP_023517000.1 (L-ascorbate oxidase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 973.4 bits (2515), Expect = 8.6e-280
Identity = 452/568 (79.58%), Postives = 490/568 (86.27%), Query Frame = 0
Query: 11 PFLVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAG 70
P L+I F+ A KI+H++WEVEYMFWSPDCVENVVMGIN QFPGPTIRANAG
Sbjct: 8 PLLLILFLFSIALTPSMALPKIKHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANAG 67
Query: 71 DTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTF 130
DTVVVELTNKLHTEGVVIHWHGILQHG+PWADGTASISQCAINPGETF YRFVVDKAGTF
Sbjct: 68 DTVVVELTNKLHTEGVVIHWHGILQHGSPWADGTASISQCAINPGETFTYRFVVDKAGTF 127
Query: 131 FYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPF 190
FYHGHLGMQRSAGLYGSLIVDP EG+ EPFHYDEEINLLLSDWWHQSVH+QEVGLSS P
Sbjct: 128 FYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHKQEVGLSSNPM 187
Query: 191 RWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPNKIYRIRIAST 250
RWIGEPQ++LINGRGQFNCSIAAKY++ + CALKG EQCAPFI+ V PNK RIRIAST
Sbjct: 188 RWIGEPQTILINGRGQFNCSIAAKYHSGSKPCALKGGEQCAPFIMHVHPNKTVRIRIAST 247
Query: 251 TALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVG 310
TALASLNFAIGNH+LLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQNPSENYWVS+G
Sbjct: 248 TALASLNFAIGNHQLLVVEADGNYVQPFRTSDIDIYSGESYSVLITTDQNPSENYWVSIG 307
Query: 311 VRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPA 370
VR R+PNTPPGLTLLNY PNS +KLP PP TP W+DF RSKNFT RI + M SP+PP
Sbjct: 308 VRARRPNTPPGLTLLNYLPNSPAKLPILPPPETPDWEDFDRSKNFTFRIFSTMSSPKPPM 367
Query: 371 TFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDY 430
F RRI LLNTQNR+NG KWAINNVSL LPPTPYLG++K+RL AF+Q PPPE FP DY
Sbjct: 368 RFDRRIFLLNTQNRLNGFTKWAINNVSLALPPTPYLGAMKYRLKTAFNQNPPPENFPSDY 427
Query: 431 DIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGE 490
+I PPPNPNTT +GVYR LG TVDVILQN+N LN SEIHPWHLHGHDFWVLGYG+
Sbjct: 428 NIDIPPPNPNTTIGNGVYRFKLGETVDVILQNANMLNPNTSEIHPWHLHGHDFWVLGYGD 487
Query: 491 GKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVV 550
GKFS +KLNLKNPPLRNT VIFP GWTA+RFVADNPGVWAFHCHIEPHLHMGMGVV
Sbjct: 488 GKFSAAVHGRKLNLKNPPLRNTVVIFPNGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVV 547
Query: 551 FAEGVHKVGNVPKQVLTCGLTGKTLMKD 579
FAEGV KVG +PK+ L CG+T K LMK+
Sbjct: 548 FAEGVEKVGRIPKKALACGVTAKALMKN 575
BLAST of Moc06g10510 vs. NCBI nr
Match:
XP_004149015.1 (L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags: Precursor [Cucumis sativus] >AAA33119.1 ascorbate oxidase precursor (EC 1.10.3.3) [Cucumis sativus] >KGN66418.1 hypothetical protein Csa_007350 [Cucumis sativus])
HSP 1 Score: 972.6 bits (2513), Expect = 1.5e-279
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0
Query: 4 FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
F +PFL FLV+ +I FG LSE KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10 FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69
Query: 64 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70 PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129
Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189
Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN L+QC L G E+CAPFIL V P
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249
Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309
Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369
Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQN 429
Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489
Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
HDFWVLGYGEGKF DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549
Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGVVFAEGVH VG +P + L CG T L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGST--ALVKNYP 584
BLAST of Moc06g10510 vs. ExPASy Swiss-Prot
Match:
P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)
HSP 1 Score: 972.6 bits (2513), Expect = 1.9e-282
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0
Query: 4 FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
F +PFL FLV+ +I FG LSE KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10 FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69
Query: 64 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70 PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129
Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189
Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN L+QC L G E+CAPFIL V P
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249
Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309
Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369
Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQN 429
Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489
Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
HDFWVLGYGEGKF DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549
Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGVVFAEGVH VG +P + L CG T L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGST--ALVKNYP 584
BLAST of Moc06g10510 vs. ExPASy Swiss-Prot
Match:
P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)
HSP 1 Score: 959.1 bits (2478), Expect = 2.2e-278
Identity = 448/579 (77.37%), Postives = 494/579 (85.32%), Query Frame = 0
Query: 1 MGIFAEPFLGPFLVIFFV-LISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQ 60
MG EP FL++FF LI AFG SEGS+IRH++WEVEYMFW+PDC EN+VMGIN Q
Sbjct: 4 MGKAREP---NFLILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQ 63
Query: 61 FPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFV 120
FPGPTIRANAGDTVVVEL NKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF
Sbjct: 64 FPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFF 123
Query: 121 YRFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVH 180
Y F VD GTFFYHGHLGMQRSAGLYGSLIVDP +G+KEPFHYD EINLLLSDWWHQS+H
Sbjct: 124 YNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIH 183
Query: 181 RQEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLP 240
+QEVGLSS P RWIGEPQ++L+NGRGQF+CSIAAKY++ L+ C LKGSE CAP+I V+P
Sbjct: 184 KQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMP 243
Query: 241 NKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQ 300
K YRIRIASTTALA+LNFAIGNH LLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQ
Sbjct: 244 KKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQ 303
Query: 301 NPSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRI 360
NPSENYWVSVG RGR PNTPPGLTLLNY PNS+SKLP+S PP TP WDDF RSKNFT RI
Sbjct: 304 NPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRI 363
Query: 361 TAAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQ 420
TAAMGSP+PP +RRI LLNTQN ING VKWAIN+VSL LPPTPYLG++KF L AFDQ
Sbjct: 364 TAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQ 423
Query: 421 KPPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLH 480
PPPE FP+DYDI PP N T +GVY+ +G VDVILQN+N + E +SEIHPWHLH
Sbjct: 424 NPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLH 483
Query: 481 GHDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHI 540
GHDFWVLGYG+GKF+ +E LNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHI
Sbjct: 484 GHDFWVLGYGDGKFT-AEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 543
Query: 541 EPHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKD 579
EPHLHMGMGVVFAEGV KVG +P + L CG T K+L+ +
Sbjct: 544 EPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINN 578
BLAST of Moc06g10510 vs. ExPASy Swiss-Prot
Match:
P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)
HSP 1 Score: 939.1 bits (2426), Expect = 2.4e-272
Identity = 430/549 (78.32%), Postives = 476/549 (86.70%), Query Frame = 0
Query: 30 SKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTVVVELTNKLHTEGVVIH 89
S+IRH++WEVEYMFW+P+C EN+VMGIN QFPGPTIRANAGD+VVVELTNKLHTEGVVIH
Sbjct: 1 SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60
Query: 90 WHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYHGHLGMQRSAGLYGSLI 149
WHGILQ GTPWADGTASISQCAINPGETF Y F VD GTFFYHGHLGMQRSAGLYGSLI
Sbjct: 61 WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120
Query: 150 VDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWIGEPQSLLINGRGQFNC 209
VDP +G+KEPFHYD EINLLLSDWWHQS+H+QEVGLSS P RWIGEPQ++L+NGRGQF+C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180
Query: 210 SIAAKYNNTLQQCALKGSEQCAPFILRVLPNKIYRIRIASTTALASLNFAIGNHKLLVVE 269
SIAAKY++ L+ C LKGSE CAP+I V P K YRIRIASTTALA+LNFAIGNH+LLVVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240
Query: 270 ADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRGRQPNTPPGLTLLNYGP 329
ADGNYVQPF T+DIDIYSGE+YSVLI TDQNPSENYWVSVG R R PNTPPGLTLLNY P
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300
Query: 330 NSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFHRRIVLLNTQNRINGAV 389
NS+SKLP+S PP TP WDDF RSKNFT RITAAMGSP+PP F+RRI LLNTQN ING V
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYV 360
Query: 390 KWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYDIHRPPPNPNTTTSSGVYR 449
KWAIN+VSL LPPTPYLG++K+ L AFDQ PPPE FP+DYDI PP N T +GVY+
Sbjct: 361 KWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQ 420
Query: 450 LDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKFSGGADEKKLNLKNPPL 509
+G VDVILQN+N + E +SE HPWHLHGHDFWVLGYG+GKFS +E LNLKNPPL
Sbjct: 421 FKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFS-AEEESSLNLKNPPL 480
Query: 510 RNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHKVGNVPKQVLTCG 569
RNT VIFPYGWTA+RFVADNPGVWAFHCHIEPHLHMGMGVVFAEGV KVG +P + L CG
Sbjct: 481 RNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACG 540
Query: 570 LTGKTLMKD 579
T K+L+ +
Sbjct: 541 GTAKSLINN 548
BLAST of Moc06g10510 vs. ExPASy Swiss-Prot
Match:
Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)
HSP 1 Score: 857.1 bits (2213), Expect = 1.2e-247
Identity = 401/573 (69.98%), Postives = 465/573 (81.15%), Query Frame = 0
Query: 8 FLGPFLVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRA 67
FL FL+ +L + +K RH +W+VEY+ WSPD E+VVMGIN QFPGPTIRA
Sbjct: 6 FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65
Query: 68 NAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKA 127
AGDTV V LTNKLHTEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETF+YRF VDKA
Sbjct: 66 KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125
Query: 128 GTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSS 187
GT+FYHGH GMQRSAGLYGSLIV+ EGEKEPFHYD E NLLLSDWWH+ H QEV LSS
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 185
Query: 188 IPFRWIGEPQSLLINGRGQFNCSIAAKYNN-TLQQCALKGSEQCAPFILRVLPNKIYRIR 247
P RWIGEPQ+LL+NGRGQ+NCS+AA+++ L QC L+G EQ AP ILRV PNKIYR+R
Sbjct: 186 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 245
Query: 248 IASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYW 307
+ASTTAL SL+ AIG HK++VVEADGNYVQPF D+DIYSGE+YSVL TDQ+P++NYW
Sbjct: 246 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 305
Query: 308 VSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSP 367
+S+ VRGR+P TP GLTLLNY PNS SK P+ PP P W+D+ SK+F+ +I A MGSP
Sbjct: 306 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSP 365
Query: 368 RPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETF 427
+PP HRRI+LLNTQN+I+G KWAINNVSL LP YLGSI++ +N AFD KPPP+ F
Sbjct: 366 KPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPDNF 425
Query: 428 PQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVL 487
P+DYD+ + PN N+T +GVY L +T+D+ILQN+NAL + VSEIHPWHLHGHDFWVL
Sbjct: 426 PKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVL 485
Query: 488 GYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMG 547
GYGEGKFS D KK NLKNPPLRNTAVIFP+GWTALRFV DNPGVWAFHCHIEPHLHMG
Sbjct: 486 GYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMG 545
Query: 548 MGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDE 580
MGV+FAEGVH V +PK+ L CGLTGK LM ++
Sbjct: 546 MGVIFAEGVHLVKKIPKEALACGLTGKMLMSNK 576
BLAST of Moc06g10510 vs. ExPASy Swiss-Prot
Match:
M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)
HSP 1 Score: 802.4 bits (2071), Expect = 3.5e-231
Identity = 370/563 (65.72%), Postives = 437/563 (77.62%), Query Frame = 0
Query: 15 IFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTVV 74
+++++ A A + + +R + WEVEY F PDC E +VM +N QFPGPTI A AGDT+V
Sbjct: 3 MWWIVAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIV 62
Query: 75 VELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYHG 134
V LTNKL TEG+VIHWHGI Q G+PWADG A ++QCAI+PGETF Y F VDK GT FYHG
Sbjct: 63 VHLTNKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHG 122
Query: 135 HLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWIG 194
H GMQRSAGLYGSLI+D A+G+KEP YD E NLLLSDWWH+ V QE+GLSS P RWIG
Sbjct: 123 HYGMQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIG 182
Query: 195 EPQSLLINGRGQFNCSIAAKYNNT-LQQCALKGSEQCAPFILRVLPNKIYRIRIASTTAL 254
E QS+LINGRGQFNCS+AA++++T L C K +QCAP L V PNK YRIR+AS+TAL
Sbjct: 183 EAQSILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTAL 242
Query: 255 ASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRG 314
ASLNFA+ HKL+VVEADGNY+ PF T+DIDIYSGE YSVL+ TDQ+PS+NY+++ GVRG
Sbjct: 243 ASLNFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRG 302
Query: 315 RQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFH 374
R+PNTPP LT+LNY S+LP+S PP TPRW+DF RSKNF+ +I AAMGSP PP TF
Sbjct: 303 RKPNTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFD 362
Query: 375 RRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYDIH 434
R++LLNTQN I G KWAINNVSL +P TPYLGS+K+ L F++ PP+ +P DYDI
Sbjct: 363 ERLILLNTQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIM 422
Query: 435 RPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKF 494
PP N N + TVDVILQN+N LN SEIHPWHLHGHDFWVLGYGEGKF
Sbjct: 423 TPPRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKF 482
Query: 495 SGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAE 554
G DEK NLKNPPLRNT ++PYGWTALRFV DNPGVW FHCHIEPHLHMGMGVVFAE
Sbjct: 483 KPGVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 542
Query: 555 GVHKVGNVPKQVLTCGLTGKTLM 577
G++++G VP + L CGLT + LM
Sbjct: 543 GLNRIGKVPDEALGCGLTKQFLM 565
BLAST of Moc06g10510 vs. ExPASy TrEMBL
Match:
Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)
HSP 1 Score: 981.1 bits (2535), Expect = 2.0e-282
Identity = 459/580 (79.14%), Postives = 499/580 (86.03%), Query Frame = 0
Query: 6 EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
+PF ++FFVL FG LSE KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7 KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66
Query: 66 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67 PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126
Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186
Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246
Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306
Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366
Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426
Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486
Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
HDFWVLGYGEGKFS D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546
Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGV+FAEGVHKVG +P + CG T ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGST--AMIKNYP 584
BLAST of Moc06g10510 vs. ExPASy TrEMBL
Match:
A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)
HSP 1 Score: 979.2 bits (2530), Expect = 7.6e-282
Identity = 458/580 (78.97%), Postives = 498/580 (85.86%), Query Frame = 0
Query: 6 EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
+PF ++FFVL FG LSE KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7 KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66
Query: 66 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67 PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126
Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186
Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246
Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
K YRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306
Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366
Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426
Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486
Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
HDFWVLGYGEGKFS D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546
Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGV+FAEGVHKVG +P + CG T ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGST--AMIKNYP 584
BLAST of Moc06g10510 vs. ExPASy TrEMBL
Match:
E7BBM8 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1)
HSP 1 Score: 972.6 bits (2513), Expect = 7.1e-280
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0
Query: 4 FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
F +PFL FLV+ +I FG LSE KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10 FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69
Query: 64 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70 PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129
Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189
Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN L+QC L G E+CAPFIL V P
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249
Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309
Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369
Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQS 429
Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489
Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
HDFWVLGYGEGKF DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549
Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGVVFAEGVH VG +P + L CG T L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGST--ALVKNYP 584
BLAST of Moc06g10510 vs. ExPASy TrEMBL
Match:
A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)
HSP 1 Score: 972.6 bits (2513), Expect = 7.1e-280
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0
Query: 4 FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
F +PFL FLV+ +I FG LSE KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10 FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69
Query: 64 PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70 PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129
Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189
Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN L+QC L G E+CAPFIL V P
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249
Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309
Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369
Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
AAMGSP+PP ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQN 429
Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489
Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
HDFWVLGYGEGKF DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549
Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
PHLHMGMGVVFAEGVH VG +P + L CG T L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGST--ALVKNYP 584
BLAST of Moc06g10510 vs. ExPASy TrEMBL
Match:
A0A6J1H925 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461608 PE=3 SV=1)
HSP 1 Score: 966.5 bits (2497), Expect = 5.1e-278
Identity = 448/568 (78.87%), Postives = 488/568 (85.92%), Query Frame = 0
Query: 11 PFLVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAG 70
PFL+I F+ A KI+H++WEVEYMFWSPDCVEN+VMGIN QFPGPTIRAN G
Sbjct: 8 PFLLILFLFSIAITPSIALPKIKHYKWEVEYMFWSPDCVENIVMGINGQFPGPTIRANVG 67
Query: 71 DTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTF 130
DTVVVELTNKLHTEGVVIHWHGILQ G+PWADGTASISQCAINPGETF YRFVVDKAGTF
Sbjct: 68 DTVVVELTNKLHTEGVVIHWHGILQRGSPWADGTASISQCAINPGETFTYRFVVDKAGTF 127
Query: 131 FYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPF 190
FYHGHLGMQRSAGLYGSLIVDP EG+ EPFHYDEEINLLLSDWWHQSVH+QEVGLSS P
Sbjct: 128 FYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHKQEVGLSSNPM 187
Query: 191 RWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPNKIYRIRIAST 250
RWIGEPQ++LINGRGQFNCSIAAKY++ + CALKG EQCAPFI+ V PNK RIRI+ST
Sbjct: 188 RWIGEPQTILINGRGQFNCSIAAKYHSGSKPCALKGGEQCAPFIMHVHPNKTVRIRISST 247
Query: 251 TALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVG 310
TALASLNFAIGNH+LLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQNPSENYWVS+G
Sbjct: 248 TALASLNFAIGNHQLLVVEADGNYVQPFRTSDIDIYSGESYSVLITTDQNPSENYWVSIG 307
Query: 311 VRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPA 370
VR R+PNTPPGLTLLNY PNS +KLP PP TP W+DF RSKNFT RI + M SP+PP
Sbjct: 308 VRARRPNTPPGLTLLNYLPNSPAKLPILPPPETPDWEDFDRSKNFTFRIFSTMSSPKPPT 367
Query: 371 TFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDY 430
F RRI LLNTQNR+NG KWAINNVSL LPPTPYLG++K+RL AF+Q PPPE FP +Y
Sbjct: 368 RFDRRIFLLNTQNRLNGFTKWAINNVSLALPPTPYLGAMKYRLKTAFNQNPPPENFPSNY 427
Query: 431 DIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGE 490
I PPPNPNTT +GVYR LG TVDVILQN+N LN SEIHPWHLHGHDFWVLGYG+
Sbjct: 428 SIDIPPPNPNTTIGNGVYRFKLGETVDVILQNANMLNPNTSEIHPWHLHGHDFWVLGYGD 487
Query: 491 GKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVV 550
GKFS +KLNLKNPPLRNT VIFP GWTA+RFVADNPGVWAFHCHIEPHLHMGMGVV
Sbjct: 488 GKFSTAVHGRKLNLKNPPLRNTVVIFPNGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVV 547
Query: 551 FAEGVHKVGNVPKQVLTCGLTGKTLMKD 579
FAEGV KVG +PK+ L CG+T K LMK+
Sbjct: 548 FAEGVEKVGRIPKKALACGVTAKALMKN 575
BLAST of Moc06g10510 vs. TAIR 10
Match:
AT5G21105.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 819.7 bits (2116), Expect = 1.5e-237
Identity = 374/565 (66.19%), Postives = 446/565 (78.94%), Query Frame = 0
Query: 14 VIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTV 73
V + VL+ A + + +R + WEVEY +WSPDC E VM +N +FPGPTI+A AGDT+
Sbjct: 19 VWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTI 78
Query: 74 VVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYH 133
VV LTNKL TEG+VIHWHGI Q G+PWADG A ++QCAINPGETF Y F V+K GT FYH
Sbjct: 79 VVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYH 138
Query: 134 GHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWI 193
GH GMQRSAGLYGSLIVD A+G+ E YD E NLLLSDWWH+++ QE+GLSS P RWI
Sbjct: 139 GHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWI 198
Query: 194 GEPQSLLINGRGQFNCSIAAKYNN--TLQQCALKGSEQCAPFILRVLPNKIYRIRIASTT 253
GE QS+LINGRGQFNCS+AA+++N +L C K +QCAP IL V PNK YRIR++STT
Sbjct: 199 GEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTT 258
Query: 254 ALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGV 313
ALASLN A+ HKL+VVEADGNY+ PF T+DIDIYSGE+YSVL+ TDQ+PS+NY++SVGV
Sbjct: 259 ALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGV 318
Query: 314 RGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPAT 373
RGR+PNT LT+LNY SKLPSS PP TPRWDDF RSKNF+ +I +AMGSP PP
Sbjct: 319 RGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKK 378
Query: 374 FHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYD 433
+ +R++LLNTQN I+G KWAINNVSL P TPYLGS+K+ L F++K PP ++ DYD
Sbjct: 379 YRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYD 438
Query: 434 IHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEG 493
I PPP PNTTT +G+Y TVDVI+QN+N L VSEIHPWHLHGHDFWVLGYG+G
Sbjct: 439 IMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDG 498
Query: 494 KFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVF 553
KF G DEK NLKNPPLRNTA+++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVF
Sbjct: 499 KFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVF 558
Query: 554 AEGVHKVGNVPKQVLTCGLTGKTLM 577
AEG++++G VP + L CGLT + LM
Sbjct: 559 AEGLNRIGKVPDEALGCGLTKQFLM 583
BLAST of Moc06g10510 vs. TAIR 10
Match:
AT5G21100.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 758.8 bits (1958), Expect = 3.1e-219
Identity = 358/564 (63.48%), Postives = 425/564 (75.35%), Query Frame = 0
Query: 18 VLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTVVVEL 77
V++ AF + S + + WEVEY +W PDC E +VM IN QFPGPTI A AGDTV++ +
Sbjct: 11 VVVVAFHSAS--AAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHV 70
Query: 78 TNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYHGHLG 137
NKL TEGVVIHWHGI Q GTPWADG A ++QC INPGETF Y+F+VDKAGT FYHGH G
Sbjct: 71 VNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYG 130
Query: 138 MQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWIGEPQ 197
MQRS+GLYG LIV KE YD E NLLLSDWWHQS+H QE+ LSS P RWIGEPQ
Sbjct: 131 MQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQ 190
Query: 198 SLLINGRGQFNCSIAAKYNNTLQQ--CALKGSEQCAPFILRVLPNKIYRIRIASTTALAS 257
SLLINGRGQFNCS AA +N ++ C K ++QCAP LRV PN++YR+RIASTTALAS
Sbjct: 191 SLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALAS 250
Query: 258 LNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRGRQ 317
LN A+ H+L+VVEADGNYV PF NDID+YSGE YSVL+ T+ PS+ YW+SVGVRGR+
Sbjct: 251 LNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGRE 310
Query: 318 PNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFHRR 377
P TP LT++NY + S+ PS PP TP W+D RSK+F+ +I AA G P+PP H +
Sbjct: 311 PKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ 370
Query: 378 IVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQD-YDIHR 437
++LLNTQN KW+INNVSL++P TPYLGSI++ L +A+D K P + D YDI +
Sbjct: 371 LILLNTQNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMK 430
Query: 438 PPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKFS 497
PPPNPNTT SG+Y G VDVILQN+N L +SEIHPWH+HGHDFWVLGYGEGKF
Sbjct: 431 PPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFK 490
Query: 498 GGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEG 557
G DEK NLKNPPLRNT V++P+GWTA+RFV DNPGVW FHCHIEPHLHMGMGVVF EG
Sbjct: 491 PGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEG 550
Query: 558 VHKVG--NVPKQVLTCGLTGKTLM 577
V ++G +P + L CGLT K LM
Sbjct: 551 VDRIGKMEIPDEALGCGLTRKWLM 568
BLAST of Moc06g10510 vs. TAIR 10
Match:
AT5G21105.3 (Plant L-ascorbate oxidase )
HSP 1 Score: 746.9 bits (1927), Expect = 1.2e-215
Identity = 342/520 (65.77%), Postives = 408/520 (78.46%), Query Frame = 0
Query: 14 VIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTV 73
V + VL+ A + + +R + WEVEY +WSPDC E VM +N +FPGPTI+A AGDT+
Sbjct: 19 VWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTI 78
Query: 74 VVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYH 133
VV LTNKL TEG+VIHWHGI Q G+PWADG A ++QCAINPGETF Y F V+K GT FYH
Sbjct: 79 VVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYH 138
Query: 134 GHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWI 193
GH GMQRSAGLYGSLIVD A+G+ E YD E NLLLSDWWH+++ QE+GLSS P RWI
Sbjct: 139 GHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWI 198
Query: 194 GEPQSLLINGRGQFNCSIAAKYNN--TLQQCALKGSEQCAPFILRVLPNKIYRIRIASTT 253
GE QS+LINGRGQFNCS+AA+++N +L C K +QCAP IL V PNK YRIR++STT
Sbjct: 199 GEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTT 258
Query: 254 ALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGV 313
ALASLN A+ HKL+VVEADGNY+ PF T+DIDIYSGE+YSVL+ TDQ+PS+NY++SVGV
Sbjct: 259 ALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGV 318
Query: 314 RGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPAT 373
RGR+PNT LT+LNY SKLPSS PP TPRWDDF RSKNF+ +I +AMGSP PP
Sbjct: 319 RGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKK 378
Query: 374 FHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYD 433
+ +R++LLNTQN I+G KWAINNVSL P TPYLGS+K+ L F++K PP ++ DYD
Sbjct: 379 YRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYD 438
Query: 434 IHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEG 493
I PPP PNTTT +G+Y TVDVI+QN+N L VSEIHPWHLHGHDFWVLGYG+G
Sbjct: 439 IMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDG 498
Query: 494 KFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPG 532
KF G DEK NLKNPPLRNTA+++PYGWTA+RFV DNPG
Sbjct: 499 KFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538
BLAST of Moc06g10510 vs. TAIR 10
Match:
AT4G39830.1 (Cupredoxin superfamily protein )
HSP 1 Score: 598.6 bits (1542), Expect = 5.4e-171
Identity = 290/569 (50.97%), Postives = 371/569 (65.20%), Query Frame = 0
Query: 13 LVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDT 72
+V+ F+ + L +G KIR +WEV+Y F SPDC E +V+ IN +FPGPTI+A GDT
Sbjct: 17 MVLCFIALFFSSVLCQG-KIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 76
Query: 73 VVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFY 132
+VVEL N TE V +HWHGI Q GTPW DG ++QC I PGE F+Y+FVVD+ GT+ Y
Sbjct: 77 IVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMY 136
Query: 133 HGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRW 192
H H GMQR +GL G + V P E EPF YD + N LL+DW+H+S+ + GL+SIPF+W
Sbjct: 137 HSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKW 196
Query: 193 IGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKG-----SEQCAPFILRVLPNKIYRIRI 252
+GEPQSL+I GRG+FNCS N T + G + C+ FIL V+P K YR+RI
Sbjct: 197 VGEPQSLMIQGRGRFNCS----NNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRI 256
Query: 253 ASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWV 312
S TAL++L+F I H L VVEADG+YV+PF ++ +YSGE YSVL+ DQNP NYW+
Sbjct: 257 GSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWI 316
Query: 313 SVGVRGRQPNTPPGLTLLNYGPNSISKLP--SSAPPPTPRWDDFARSKNFTTRITAAMGS 372
+ + R TPP +LNY PN + P S + P W+D + I A G
Sbjct: 317 TSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGF 376
Query: 373 PRP-PATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK-PPP 432
P + IVLLNTQN +NG +W++NNVS P TPYL ++K L AFD + P
Sbjct: 377 IHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAP 436
Query: 433 ETF-PQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHD 492
E + ++YDI P N N TTS G+YRL STVDVILQN+N +N SE HPWHLHGHD
Sbjct: 437 ENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHD 496
Query: 493 FWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPH 552
FWVLGYGEGKF+ D K+ N +P +NT + P+GWTALRF ADNPGVW+FHCHIE H
Sbjct: 497 FWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESH 556
Query: 553 LHMGMGVVFAEGVHKVGNVPKQVLTCGLT 572
MGMG+VF G+ KV ++P ++ CG T
Sbjct: 557 FFMGMGIVFESGIDKVSSLPSSIMGCGQT 580
BLAST of Moc06g10510 vs. TAIR 10
Match:
AT5G21105.2 (Plant L-ascorbate oxidase )
HSP 1 Score: 556.2 bits (1432), Expect = 3.1e-158
Identity = 255/382 (66.75%), Postives = 306/382 (80.10%), Query Frame = 0
Query: 197 QSLLINGRGQFNCSIAAKYNN--TLQQCALKGSEQCAPFILRVLPNKIYRIRIASTTALA 256
+S+LINGRGQFNCS+AA+++N +L C K +QCAP IL V PNK YRIR++STTALA
Sbjct: 11 KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70
Query: 257 SLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRGR 316
SLN A+ HKL+VVEADGNY+ PF T+DIDIYSGE+YSVL+ TDQ+PS+NY++SVGVRGR
Sbjct: 71 SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130
Query: 317 QPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFHR 376
+PNT LT+LNY SKLPSS PP TPRWDDF RSKNF+ +I +AMGSP PP + +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190
Query: 377 RIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYDIHR 436
R++LLNTQN I+G KWAINNVSL P TPYLGS+K+ L F++K PP ++ DYDI
Sbjct: 191 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMN 250
Query: 437 PPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKFS 496
PPP PNTTT +G+Y TVDVI+QN+N L VSEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 251 PPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFK 310
Query: 497 GGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEG 556
G DEK NLKNPPLRNTA+++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVFAEG
Sbjct: 311 PGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEG 370
Query: 557 VHKVGNVPKQVLTCGLTGKTLM 577
++++G VP + L CGLT + LM
Sbjct: 371 LNRIGKVPDEALGCGLTKQFLM 392
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038878978.1 | 4.7e-286 | 79.59 | L-ascorbate oxidase [Benincasa hispida] | [more] |
| NP_001284441.1 | 4.1e-282 | 79.14 | L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis me... | [more] |
| KAA0055636.1 | 1.6e-281 | 78.97 | L-ascorbate oxidase [Cucumis melo var. makuwa] | [more] |
| XP_023517000.1 | 8.6e-280 | 79.58 | L-ascorbate oxidase-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_004149015.1 | 1.5e-279 | 78.97 | L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidas... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| P14133 | 1.9e-282 | 78.97 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1 | [more] |
| P24792 | 2.2e-278 | 77.37 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2 | [more] |
| P37064 | 2.4e-272 | 78.32 | L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1 | [more] |
| Q40588 | 1.2e-247 | 69.98 | L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1 | [more] |
| M4DUF2 | 3.5e-231 | 65.72 | L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9M5B4 | 2.0e-282 | 79.14 | L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1 | [more] |
| A0A5A7UMY3 | 7.6e-282 | 78.97 | L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G... | [more] |
| E7BBM8 | 7.1e-280 | 78.97 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1 | [more] |
| A0A0A0LXB9 | 7.1e-280 | 78.97 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1 | [more] |
| A0A6J1H925 | 5.1e-278 | 78.87 | L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461608 PE=3 SV=1 | [more] |
