Moc06g09500 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g09500
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionsubtilisin-like protease SBT1.8
Locationchr6: 6944829 .. 6947309 (+)
RNA-Seq ExpressionMoc06g09500
SyntenyMoc06g09500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGCCATAGATCGATTACTCATCGCTTCCCTTCTTCTTCTTCTTCCTTGTGTCTTCGTCGATGCCAAGAGAACCTACATTGTTCGCATGAAACATCATGCTCTGCCTTCTGATTACCTCACCCACCATGACTGGTACTCTGCCAATCTTCAATCTCTCTCCTCCTCCACCACCTCCGATTCCCTTCTCTACACCTACACCGCCGCCTACCATGGCTTCGCAGCTTCTTTAGATCCAGAAGAAGCCGAATTGCTCCGCCAATCCGATTCAGTTCTGGGTGTCTATGAAGACACTGTTTACAACCTCCACACCACTCGCACTCCGGGGTTCCTCGGCCTTGACTCCGATTTCGGCTTATGGGAAGGTCACAACACTCAGGATCTTGACCAGGCCTCCCACGATGTCATCATTGGGGTCCTCGACACTGGGATTTGGCCGGAATCGAAGAGCTTCGATGACACCGGTATGCCGGAGATCCCATCACGGTGGCGCGGAGAATGCGAATCGGGACCCGATTTCAGCCCTTCGCTATGCAACAAGAAATTAATCGGAGCTCGTAGCTTCTCCAAAGGTTATCAGATGGCCTCCGGTGGTGGATACTTCAGAAAACCCAGAGAGAATCAGTCGCCTCGAGACCAGGACGGGCACGGCACGCACACGGCGAGCACGGCCGCTGGCTCACACGTGGCTAACGCGAGCTTACTCGGGTATGCCAGAGGCATTGCCCGTGGGATGGCTCCTCAAGCGCGAGTTGCCGCTTACAAGACCTGTTGGCCCACTGGTTGCTTTGGATCTGACATTTTAGCGGGTATGGATCGGGCGATATCAGACGGCGTCGATGTGCTCTCGCTCTCCTTAGGCGGTGGCTCGGCGCCGTACTACCGTGATACCATCGCAATTGGGGCATTTGCGGCCATGGAGAAGGGGGTTTTCGTTTCGTGCTCGGCCGGGAACAGTGGGCCAAACAGGGCTTCCCTGGCCAATGTGGCGCCGTGGATCATGACGGTCGGAGCCGGAACTCTCGACCGGGACTTTCCGGCTTATGTCCAATTGGGAAACGGCAAACGGTTCACCGGAGTGTCGCTCTACAGCGGCCAAGGGATGGGGAATAAGCCAGTGGAGTTGGTGTATGACAAAGGAAGCAATACATCCAGTAACATGTGCTTGCCCGGATCTCTGGAGCCGGCGGTGGTCCGGGGGAAGGTGGTGATCTGCGACAGAGGAATCAATGCCAGAGTAGAGAAGGGAGGGGTGGTGCGGGATGCAGGTGGCATAGGGATGATTCTGGCGAACACCGCCGCAAGTGGGGAGGAATTGGTGGCGGACAGCCACTTGCTGCCGGCCGTCGCGGTGGGGAGAAAAACCGGCGACTTGATCCGGCAGTATGTGAAGTCAGTTACAAAGCCGACGGCGGTGCTGAGCTTTGGTGGGACGGTTTTGAATGTGCGGCCATCTCCAGTGGTGGCAGCCTTCAGTTCCAGAGGACCCAACTTGGTAACGCCCCAAATCCTGAAGCCAGACGTTATTGGGCCTGGCGTTAACATTTTGGCCGCCTGGTCTGAGTCCATTGGGCCTACTGGATTGGACAGCGACAAGAGGAAAACCCAGTTCAATATTATGTCAGGTATGTTGTTTGTCTTCAAAGGCTGCCTTTTTCTTTCCCTTTTCTGTCATCCAACGGCTGTCCTGGCGCTTTCAAGATTTCAAATTTATGACTTGCCCTTTTAACTTTTCTGAAAGCTTTGATTCGGTTTTCTAGGCAAATAATGTTAGAATCTTCTGCTAAAGTGTCCTTTGTGCTTGAAAAATGTAGGAACATCCATGTCCTGCCCTCATATAAGTGGGCTGGCGGCATTGCTGAAGGCAGCTCATCCGCAATGGAGTCCTAGTGCTATCAAATCTGCGTTGATGACCACTGCATACACGCAGGACAACACCAACTCATCTCTCCGGGACGCTGCCGGAGGGGCATTTTCCAATCCGTGGGCTCATGGAGCTGGCCATGTTGATCCCCACAAGGCCCTATCCCCCGGTCTCCTGTACGATATCTCGACCGAGGATTACATTGCCTTTTTGTGCTCTTTGGACTATGGTATTGACCATGTTCAGGCTATCGTGAAGCGCCCGAACATAACCTGCTCCAGAAAGTTTGCTGACCCAGGACAACTCAACTACCCGTCGTTTTCAGTTGTGTTTGGGAGCAAGAGGGTTGTTCGATACACTCGAATAGTCACAAATGTAGGGGCTGCAGGCTCGGTTTACGAAGTGGCTACAACTGCACCTTCAGTTGTGAAGGTGGTGGTGAAACCTACAAAGCTTGCATTCACTAAAGTTGGGGAGAGGAAGAGATATACTGTTACATTCGTGGCAAGCAGCAATGCAGCTCAAACCACGAGATTCGGGTTTGGATCGATTGCATGGAGCAACGACCAACACCAAGTCAGAAGCCCGGTGGCATTCGCCTGGACGAGGTTGTGA

mRNA sequence

ATGGGGGCCATAGATCGATTACTCATCGCTTCCCTTCTTCTTCTTCTTCCTTGTGTCTTCGTCGATGCCAAGAGAACCTACATTGTTCGCATGAAACATCATGCTCTGCCTTCTGATTACCTCACCCACCATGACTGGTACTCTGCCAATCTTCAATCTCTCTCCTCCTCCACCACCTCCGATTCCCTTCTCTACACCTACACCGCCGCCTACCATGGCTTCGCAGCTTCTTTAGATCCAGAAGAAGCCGAATTGCTCCGCCAATCCGATTCAGTTCTGGGTGTCTATGAAGACACTGTTTACAACCTCCACACCACTCGCACTCCGGGGTTCCTCGGCCTTGACTCCGATTTCGGCTTATGGGAAGGTCACAACACTCAGGATCTTGACCAGGCCTCCCACGATGTCATCATTGGGGTCCTCGACACTGGGATTTGGCCGGAATCGAAGAGCTTCGATGACACCGGTATGCCGGAGATCCCATCACGGTGGCGCGGAGAATGCGAATCGGGACCCGATTTCAGCCCTTCGCTATGCAACAAGAAATTAATCGGAGCTCGTAGCTTCTCCAAAGGTTATCAGATGGCCTCCGGTGGTGGATACTTCAGAAAACCCAGAGAGAATCAGTCGCCTCGAGACCAGGACGGGCACGGCACGCACACGGCGAGCACGGCCGCTGGCTCACACGTGGCTAACGCGAGCTTACTCGGGTATGCCAGAGGCATTGCCCGTGGGATGGCTCCTCAAGCGCGAGTTGCCGCTTACAAGACCTGTTGGCCCACTGGTTGCTTTGGATCTGACATTTTAGCGGGTATGGATCGGGCGATATCAGACGGCGTCGATGTGCTCTCGCTCTCCTTAGGCGGTGGCTCGGCGCCGTACTACCGTGATACCATCGCAATTGGGGCATTTGCGGCCATGGAGAAGGGGGTTTTCGTTTCGTGCTCGGCCGGGAACAGTGGGCCAAACAGGGCTTCCCTGGCCAATGTGGCGCCGTGGATCATGACGGTCGGAGCCGGAACTCTCGACCGGGACTTTCCGGCTTATGTCCAATTGGGAAACGGCAAACGGTTCACCGGAGTGTCGCTCTACAGCGGCCAAGGGATGGGGAATAAGCCAGTGGAGTTGGTGTATGACAAAGGAAGCAATACATCCAGTAACATGTGCTTGCCCGGATCTCTGGAGCCGGCGGTGGTCCGGGGGAAGGTGGTGATCTGCGACAGAGGAATCAATGCCAGAGTAGAGAAGGGAGGGGTGGTGCGGGATGCAGGTGGCATAGGGATGATTCTGGCGAACACCGCCGCAAGTGGGGAGGAATTGGTGGCGGACAGCCACTTGCTGCCGGCCGTCGCGGTGGGGAGAAAAACCGGCGACTTGATCCGGCAGTATGTGAAGTCAGTTACAAAGCCGACGGCGGTGCTGAGCTTTGGTGGGACGGTTTTGAATGTGCGGCCATCTCCAGTGGTGGCAGCCTTCAGTTCCAGAGGACCCAACTTGGTAACGCCCCAAATCCTGAAGCCAGACGTTATTGGGCCTGGCGTTAACATTTTGGCCGCCTGGTCTGAGTCCATTGGGCCTACTGGATTGGACAGCGACAAGAGGAAAACCCAGTTCAATATTATGTCAGGAACATCCATGTCCTGCCCTCATATAAGTGGGCTGGCGGCATTGCTGAAGGCAGCTCATCCGCAATGGAGTCCTAGTGCTATCAAATCTGCGTTGATGACCACTGCATACACGCAGGACAACACCAACTCATCTCTCCGGGACGCTGCCGGAGGGGCATTTTCCAATCCGTGGGCTCATGGAGCTGGCCATGTTGATCCCCACAAGGCCCTATCCCCCGGTCTCCTGTACGATATCTCGACCGAGGATTACATTGCCTTTTTGTGCTCTTTGGACTATGGTATTGACCATGTTCAGGCTATCGTGAAGCGCCCGAACATAACCTGCTCCAGAAAGTTTGCTGACCCAGGACAACTCAACTACCCGTCGTTTTCAGTTGTGTTTGGGAGCAAGAGGGTTGTTCGATACACTCGAATAGTCACAAATGTAGGGGCTGCAGGCTCGGTTTACGAAGTGGCTACAACTGCACCTTCAGTTGTGAAGGTGGTGGTGAAACCTACAAAGCTTGCATTCACTAAAGTTGGGGAGAGGAAGAGATATACTGTTACATTCGTGGCAAGCAGCAATGCAGCTCAAACCACGAGATTCGGGTTTGGATCGATTGCATGGAGCAACGACCAACACCAAGTCAGAAGCCCGGTGGCATTCGCCTGGACGAGGTTGTGA

Coding sequence (CDS)

ATGGGGGCCATAGATCGATTACTCATCGCTTCCCTTCTTCTTCTTCTTCCTTGTGTCTTCGTCGATGCCAAGAGAACCTACATTGTTCGCATGAAACATCATGCTCTGCCTTCTGATTACCTCACCCACCATGACTGGTACTCTGCCAATCTTCAATCTCTCTCCTCCTCCACCACCTCCGATTCCCTTCTCTACACCTACACCGCCGCCTACCATGGCTTCGCAGCTTCTTTAGATCCAGAAGAAGCCGAATTGCTCCGCCAATCCGATTCAGTTCTGGGTGTCTATGAAGACACTGTTTACAACCTCCACACCACTCGCACTCCGGGGTTCCTCGGCCTTGACTCCGATTTCGGCTTATGGGAAGGTCACAACACTCAGGATCTTGACCAGGCCTCCCACGATGTCATCATTGGGGTCCTCGACACTGGGATTTGGCCGGAATCGAAGAGCTTCGATGACACCGGTATGCCGGAGATCCCATCACGGTGGCGCGGAGAATGCGAATCGGGACCCGATTTCAGCCCTTCGCTATGCAACAAGAAATTAATCGGAGCTCGTAGCTTCTCCAAAGGTTATCAGATGGCCTCCGGTGGTGGATACTTCAGAAAACCCAGAGAGAATCAGTCGCCTCGAGACCAGGACGGGCACGGCACGCACACGGCGAGCACGGCCGCTGGCTCACACGTGGCTAACGCGAGCTTACTCGGGTATGCCAGAGGCATTGCCCGTGGGATGGCTCCTCAAGCGCGAGTTGCCGCTTACAAGACCTGTTGGCCCACTGGTTGCTTTGGATCTGACATTTTAGCGGGTATGGATCGGGCGATATCAGACGGCGTCGATGTGCTCTCGCTCTCCTTAGGCGGTGGCTCGGCGCCGTACTACCGTGATACCATCGCAATTGGGGCATTTGCGGCCATGGAGAAGGGGGTTTTCGTTTCGTGCTCGGCCGGGAACAGTGGGCCAAACAGGGCTTCCCTGGCCAATGTGGCGCCGTGGATCATGACGGTCGGAGCCGGAACTCTCGACCGGGACTTTCCGGCTTATGTCCAATTGGGAAACGGCAAACGGTTCACCGGAGTGTCGCTCTACAGCGGCCAAGGGATGGGGAATAAGCCAGTGGAGTTGGTGTATGACAAAGGAAGCAATACATCCAGTAACATGTGCTTGCCCGGATCTCTGGAGCCGGCGGTGGTCCGGGGGAAGGTGGTGATCTGCGACAGAGGAATCAATGCCAGAGTAGAGAAGGGAGGGGTGGTGCGGGATGCAGGTGGCATAGGGATGATTCTGGCGAACACCGCCGCAAGTGGGGAGGAATTGGTGGCGGACAGCCACTTGCTGCCGGCCGTCGCGGTGGGGAGAAAAACCGGCGACTTGATCCGGCAGTATGTGAAGTCAGTTACAAAGCCGACGGCGGTGCTGAGCTTTGGTGGGACGGTTTTGAATGTGCGGCCATCTCCAGTGGTGGCAGCCTTCAGTTCCAGAGGACCCAACTTGGTAACGCCCCAAATCCTGAAGCCAGACGTTATTGGGCCTGGCGTTAACATTTTGGCCGCCTGGTCTGAGTCCATTGGGCCTACTGGATTGGACAGCGACAAGAGGAAAACCCAGTTCAATATTATGTCAGGAACATCCATGTCCTGCCCTCATATAAGTGGGCTGGCGGCATTGCTGAAGGCAGCTCATCCGCAATGGAGTCCTAGTGCTATCAAATCTGCGTTGATGACCACTGCATACACGCAGGACAACACCAACTCATCTCTCCGGGACGCTGCCGGAGGGGCATTTTCCAATCCGTGGGCTCATGGAGCTGGCCATGTTGATCCCCACAAGGCCCTATCCCCCGGTCTCCTGTACGATATCTCGACCGAGGATTACATTGCCTTTTTGTGCTCTTTGGACTATGGTATTGACCATGTTCAGGCTATCGTGAAGCGCCCGAACATAACCTGCTCCAGAAAGTTTGCTGACCCAGGACAACTCAACTACCCGTCGTTTTCAGTTGTGTTTGGGAGCAAGAGGGTTGTTCGATACACTCGAATAGTCACAAATGTAGGGGCTGCAGGCTCGGTTTACGAAGTGGCTACAACTGCACCTTCAGTTGTGAAGGTGGTGGTGAAACCTACAAAGCTTGCATTCACTAAAGTTGGGGAGAGGAAGAGATATACTGTTACATTCGTGGCAAGCAGCAATGCAGCTCAAACCACGAGATTCGGGTTTGGATCGATTGCATGGAGCAACGACCAACACCAAGTCAGAAGCCCGGTGGCATTCGCCTGGACGAGGTTGTGA

Protein sequence

MGAIDRLLIASLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL
Homology
BLAST of Moc06g09500 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 727/766 (94.91%), Postives = 747/766 (97.52%), Query Frame = 0

Query: 1   MGAIDRLLIAS---LLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSS 60
           MG++ RLL+AS   LLLLLPCVFV+AKRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  TTSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
            TSDSLLYTYT+AYHGFAASLDP+EAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPV LVY+KGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLLYDIST DYI FLCSLDYGIDHVQAIVKR NITCS+KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKV VKP+KL F+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           ERKRYTVTFVAS +AA+TTR+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Moc06g09500 vs. NCBI nr
Match: KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1453.7 bits (3762), Expect = 0.0e+00
Identity = 726/765 (94.90%), Postives = 747/765 (97.65%), Query Frame = 0

Query: 1   MGAIDRLLIAS--LLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSST 60
           MG++ RLL+AS  LLLLLPCVFV+AKRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS 
Sbjct: 1   MGSMARLLVASFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60

Query: 61  TSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           TSDSLLYTYT+AYHGFAASLDP+EAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNKPV LVY+KGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGL+YDIST DYI FLCSLDYGIDHVQAI KR NITC +KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720

Query: 721 RKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           RKRYTVTFVAS +AA+TTR+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Moc06g09500 vs. NCBI nr
Match: XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1453.3 bits (3761), Expect = 0.0e+00
Identity = 726/766 (94.78%), Postives = 747/766 (97.52%), Query Frame = 0

Query: 1   MGAIDRLLIAS---LLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSS 60
           MG++ RLL+AS   LLLLLPCVFV+AKRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  TTSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
            TSDSLLYTYT+AYHGFAASLDP+EAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPV LVY+KGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDIST DYI FLCSLDYGIDHVQAI KR NITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKV VKP+KL F+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           ERKRYTVTFVAS +AA+TTR+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Moc06g09500 vs. NCBI nr
Match: XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1449.5 bits (3751), Expect = 0.0e+00
Identity = 724/765 (94.64%), Postives = 745/765 (97.39%), Query Frame = 0

Query: 1   MGAIDRLLIA--SLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSST 60
           MG++  LL+A   LLLLLPCVFV+AKRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS 
Sbjct: 1   MGSMASLLVAYFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60

Query: 61  TSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           TSDSLLYTYT+AYHGFAASLDP+EAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNKPV LVY+KGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGL+YDIST DYI FLCSLDYGIDHVQAI KR NITCS+KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCSKKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720

Query: 721 RKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           RKRYTVTFVAS +AA+TTR+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Moc06g09500 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1446.0 bits (3742), Expect = 0.0e+00
Identity = 722/763 (94.63%), Postives = 746/763 (97.77%), Query Frame = 0

Query: 1   MGAIDRLLIASLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTS 60
           MG++ RLLIA LLLLLPCVFV+AK+TYIVRMKH+ALPS+YLTHHDWYSA+LQSLSSS+TS
Sbjct: 1   MGSMARLLIAFLLLLLPCVFVNAKKTYIVRMKHNALPSEYLTHHDWYSAHLQSLSSSSTS 60

Query: 61  DSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGL 120
           DSLLYTYT+AYHGFAASLD  EAELLRQSDSVLGVYED+VY LHTTRTPGFLGLDSDFGL
Sbjct: 61  DSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSDFGL 120

Query: 121 WEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCN 180
           WEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESG DFSPSLCN
Sbjct: 121 WEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADFSPSLCN 180

Query: 181 KKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYAR 240
           KKLIGARSFSKGYQMASGGGYFRKPREN+SPRDQDGHGTHTASTAAGSHVANASLLGYAR
Sbjct: 181 KKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYAR 240

Query: 241 GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIA 300
           GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIA
Sbjct: 241 GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIA 300

Query: 301 IGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTG 360
           IGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQ+GNGKRFTG
Sbjct: 301 IGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKRFTG 360

Query: 361 VSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVV 420
           VSLYSGQGMG+K VELVY+KGSNTSSNMCLPGSLEPAVVRGKVV+CDRGINARVEKGGVV
Sbjct: 361 VSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGGVV 420

Query: 421 RDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTV 480
           RDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGGT+
Sbjct: 421 RDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTI 480

Query: 481 LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNI 540
           LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNI
Sbjct: 481 LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNI 540

Query: 541 MSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPW 600
           MSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPW
Sbjct: 541 MSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPW 600

Query: 601 AHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQ 660
           AHGAGHVDPHKALSPGLLYDIST DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADPGQ
Sbjct: 601 AHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQ 660

Query: 661 LNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERK 720
           LNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKV VKP+KL FTKVGERK
Sbjct: 661 LNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVGERK 720

Query: 721 RYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           RYTVTFVAS +AAQT RFGFGSIAW+N QHQVRSPVAFAWTRL
Sbjct: 721 RYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVRSPVAFAWTRL 763

BLAST of Moc06g09500 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 550/756 (72.75%), Postives = 638/756 (84.39%), Query Frame = 0

Query: 8   LIASLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTY 67
           +I + L LL  +   AK+TYI+R+ H   P  +LTHHDWY++ L S S      SLLYTY
Sbjct: 13  IITTFLFLL--LHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTY 72

Query: 68  TAAYHGFAASLDPEEAE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNT 127
           T ++HGF+A LD  EA+ LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+      
Sbjct: 73  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------ 132

Query: 128 QDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGA 187
            DL  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+GECESG DF   LCNKKLIGA
Sbjct: 133 HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGA 192

Query: 188 RSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGM 247
           RSFSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGM
Sbjct: 193 RSFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 252

Query: 248 APQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAA 307
           A +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGAF+A
Sbjct: 253 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSA 312

Query: 308 MEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSG 367
           ME+GVFVSCSAGNSGP RAS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG
Sbjct: 313 MERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG 372

Query: 368 QGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGI 427
            GMG KP+ELVY+KG+++SSN+CLPGSL+ ++VRGK+V+CDRG+NARVEKG VVRDAGG+
Sbjct: 373 VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 432

Query: 428 GMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPS 487
           GMI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YVKS +KPTA+L F GTVL+V+PS
Sbjct: 433 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 492

Query: 488 PVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSM 547
           PVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSM
Sbjct: 493 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 552

Query: 548 SCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGH 607
           SCPHISGLA LLKAAHP+WSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GH
Sbjct: 553 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 612

Query: 608 VDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSF 667
           VDP KALSPGL+YDISTE+YI FLCSLDY +DH+ AIVKRP++ CS+KF+DPGQLNYPSF
Sbjct: 613 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 672

Query: 668 SVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTF 727
           SV+FG KRVVRYTR VTNVGAA SVY+V       V + VKP+KL+F  VGE+KRYTVTF
Sbjct: 673 SVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTF 732

Query: 728 VASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTR 763
           V+    + T +  FGSI WSN QH+VRSPVAF+W R
Sbjct: 733 VSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Moc06g09500 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 9.8e-234
Identity = 423/742 (57.01%), Postives = 533/742 (71.83%), Query Frame = 0

Query: 26  TYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAEL 85
           TYIV M    +PS +  H +WY ++L+S+S S     LLYTY  A HGF+  L  EEA+ 
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLYTYENAIHGFSTRLTQEEADS 90

Query: 86  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDL--DQASH-DVIIGVLD 145
           L     V+ V  +  Y LHTTRTP FLGLD         +T DL  +  S+ DV++GVLD
Sbjct: 91  LMTQPGVISVLPEHRYELHTTRTPLFLGLD--------EHTADLFPEAGSYSDVVVGVLD 150

Query: 146 TGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYF 205
           TG+WPESKS+ D G   IPS W+G CE+G +F+ SLCN+KLIGAR F++GY+  S  G  
Sbjct: 151 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE--STMGPI 210

Query: 206 RKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTG 265
            + +E++SPRD DGHGTHT+STAAGS V  ASLLGYA G ARGMAP+ARVA YK CW  G
Sbjct: 211 DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGG 270

Query: 266 CFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGP 325
           CF SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGAFAAME+G+ VSCSAGN+GP
Sbjct: 271 CFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGP 330

Query: 326 NRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVY--DK 385
           + +SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL+ G+ + +K +  +Y  + 
Sbjct: 331 SSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA 390

Query: 386 GSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEEL 445
            + T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+ AGG+GMILANTAA+GEEL
Sbjct: 391 SNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEEL 450

Query: 446 VADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLV 505
           VAD+HLLPA  VG K GD+IR YV +   PTA +S  GTV+ V+PSPVVAAFSSRGPN +
Sbjct: 451 VADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSI 510

Query: 506 TPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKA 565
           TP ILKPD+I PGVNILAAW+ + GPTGL SD R+ +FNI+SGTSMSCPH+SGLAALLK+
Sbjct: 511 TPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKS 570

Query: 566 AHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYD 625
            HP+WSP+AI+SALMTTAY        L D A G  S P+ HGAGHV P  A +PGL+YD
Sbjct: 571 VHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYD 630

Query: 626 ISTEDYIAFLCSLDYGIDHVQAIVKRPNITCS-RKFADPGQLNYPSFSVVFGSKRVVRYT 685
           ++TEDY+ FLC+L+Y    ++++ +R N TC   K      LNYPSF+V        +YT
Sbjct: 631 LTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYT 690

Query: 686 RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFG 745
           R VT+VG AG+     T+  + VK+ V+P  L F +  E+K YTVTF   S+    +   
Sbjct: 691 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-S 750

Query: 746 FGSIAWSNDQHQVRSPVAFAWT 762
           FGSI WS+ +H V SPVA +WT
Sbjct: 751 FGSIEWSDGKHVVGSPVAISWT 757

BLAST of Moc06g09500 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 8.6e-222
Identity = 414/775 (53.42%), Postives = 524/775 (67.61%), Query Frame = 0

Query: 6   RLLIASLLLLLP---CVF------VDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSS 65
           +L ++S+  + P   C F       D   +YIV ++    PS + +H++W+ + L+SL S
Sbjct: 3   KLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPS 62

Query: 66  STTSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 125
           S    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D    +HTT TP FLG   
Sbjct: 63  SPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQ 122

Query: 126 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSP 185
           + GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+GECE GPDF  
Sbjct: 123 NSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPA 182

Query: 186 SLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLL 245
           S CN+KLIGAR+F +GY     G      +E++SPRD +GHGTHTASTAAGS VANASL 
Sbjct: 183 SSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLY 242

Query: 246 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP-Y 305
            YARG A GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP Y
Sbjct: 243 QYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEY 302

Query: 306 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGN 365
           + D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    G+
Sbjct: 303 HTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGD 362

Query: 366 GKRFTGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARV 425
           GK FTG SLY+G+ + +  + LVY    +  S +C PG L  ++V GK+V+CDRG NARV
Sbjct: 363 GKVFTGTSLYAGESLPDSQLSLVY--SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARV 422

Query: 426 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVL 485
           EKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+K+   PTA +
Sbjct: 423 EKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKI 482

Query: 486 SFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDK 545
           SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD D 
Sbjct: 483 SFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDP 542

Query: 546 RKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAG 605
           R+ QFNI+SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D A 
Sbjct: 543 RRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLAT 602

Query: 606 GAFSNPWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNI--TC 665
           G  SN + HGAGHVDP+KAL+PGL+YDI  ++Y+AFLC++ Y    +   ++ P +   C
Sbjct: 603 GKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDAC 662

Query: 666 -SRKFADPGQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYEVATTAPSVVKVVVKP 725
            + K    G LNYPSFSVVF S   VV+Y R+V NVG+   +VYEV   +P+ V++ V P
Sbjct: 663 ETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSP 722

Query: 726 TKLAFTKVGERKRYTVTF---VASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAW 761
           +KLAF+K      Y VTF   V            FGSI W++ +H V+SPVA  W
Sbjct: 723 SKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Moc06g09500 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 7.8e-215
Identity = 391/756 (51.72%), Postives = 504/756 (66.67%), Query Frame = 0

Query: 21  VDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDS------LLYTYTAAYHGF 80
           +  K+TY++ M   A+P  Y  H  WYS+ + S++   + +       +LYTY  A+HG 
Sbjct: 31  ISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGL 90

Query: 81  AASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASH 140
           AA L  EEAE L + D V+ V  +T Y LHTTR+P FLGL+      E           H
Sbjct: 91  AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ----ESERVWAERVTDH 150

Query: 141 DVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQ 200
           DV++GVLDTGIWPES+SF+DTGM  +P+ WRG CE+G  F    CN+K++GAR F +GY+
Sbjct: 151 DVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYE 210

Query: 201 MASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAA 260
            A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+L G+A G ARGMA +ARVAA
Sbjct: 211 AAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAA 270

Query: 261 YKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVS 320
           YK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y RD+++I  F AME GVFVS
Sbjct: 271 YKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVS 330

Query: 321 CSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGM--GNK 380
           CSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G  + F GVSLY G+ +   NK
Sbjct: 331 CSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNK 390

Query: 381 PVELVYDKGSNTSS----NMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGM 440
              LVY  G N SS    + CL G+L+   V GK+VICDRG+  RV+KG VV+ AGGIGM
Sbjct: 391 QYPLVY-LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGM 450

Query: 441 ILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPV 500
           +L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K TA L   GT + ++PSPV
Sbjct: 451 VLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPV 510

Query: 501 VAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSC 560
           VAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD R+ +FNI+SGTSMSC
Sbjct: 511 VAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSC 570

Query: 561 PHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVD 620
           PH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L DA+G A S+P+ HGAGH+D
Sbjct: 571 PHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHID 630

Query: 621 PHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFA-DPGQLNYPSFS 680
           P +A  PGL+YDI  ++Y  FLC+ D     ++   K  N TC    A +PG LNYP+ S
Sbjct: 631 PLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAIS 690

Query: 681 VVFGSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTV 740
            +F      + +   R VTNVG   S Y+V+ +      V V+P  L FT   ++  YTV
Sbjct: 691 ALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTV 750

Query: 741 TFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAW 761
           TF       +  R  FG + W +  H+VRSPV   W
Sbjct: 751 TF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Moc06g09500 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 1.1e-208
Identity = 386/762 (50.66%), Postives = 511/762 (67.06%), Query Frame = 0

Query: 12  LLLLLPCVFV-----DAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYT 71
           LLL L   F+      A +T+I R+   ++PS + TH+ WYS      S       +++ 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIVHV 67

Query: 72  YTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNT 131
           Y   +HGF+A + P+EA+ LR   +VL V+ED    LHTTR+P FLGL +  GLW     
Sbjct: 68  YHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW----- 127

Query: 132 QDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGA 191
            + D  S DVIIGV DTGIWPE +SF D  +  IP RWRG CESG  FSP  CN+K+IGA
Sbjct: 128 SESDYGS-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGA 187

Query: 192 RSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGM 251
           R F+KG Q A  GG   K  E  SPRD DGHGTHT+STAAG H   AS+ GYA G+A+G+
Sbjct: 188 RFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 247

Query: 252 APQARVAAYKTCW-PTGCFGSDILAGMDRAISDGVDVLSLSLGGG---SAPYYRDTIAIG 311
           AP+AR+AAYK CW  +GC  SDILA  D A+ DGVDV+S+S+GGG   ++PYY D IAIG
Sbjct: 248 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 307

Query: 312 AFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVS 371
           ++ A  KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R  GVS
Sbjct: 308 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 367

Query: 372 LYSGQGMGNKPVELVY-DKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVR 431
           LY+G  +  +   +VY  K   +S+++C+  +L+P  VRGK+VICDRG + RV KG VV+
Sbjct: 368 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 427

Query: 432 DAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVL 491
            AGG+GMILAN A++GE LV D+HL+PA AVG   GD I+ Y  S   P A + F GT++
Sbjct: 428 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 487

Query: 492 NVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIM 551
            ++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL SD RKT+FNI+
Sbjct: 488 GIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 547

Query: 552 SGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWA 611
           SGTSM+CPH+SG AALLK+AHP WSP+ I+SA+MTT    DN+N SL D + G  + P+ 
Sbjct: 548 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYD 607

Query: 612 HGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQL 671
           +G+GH++  +A++PGL+YDI+ +DYI FLCS+ YG   +Q I + P    + +   PG L
Sbjct: 608 YGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNL 667

Query: 672 NYPSFSVVFGSKR--VVRYT--RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVG 731
           NYPS + VF + R  +V  T  R  TNVG A +VY     +P  V V VKP +L FT   
Sbjct: 668 NYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAV 727

Query: 732 ERKRYTVTFVASSNAAQTTRFG--FGSIAW-SNDQHQVRSPV 757
           +R+ Y VT   ++        G  FGS+ W    +H VRSP+
Sbjct: 728 KRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756

BLAST of Moc06g09500 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 727/766 (94.91%), Postives = 747/766 (97.52%), Query Frame = 0

Query: 1   MGAIDRLLIAS---LLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSS 60
           MG++ RLL+AS   LLLLLPCVFV+AKRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  TTSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
            TSDSLLYTYT+AYHGFAASLDP+EAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPV LVY+KGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLLYDIST DYI FLCSLDYGIDHVQAIVKR NITCS+KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKV VKP+KL F+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           ERKRYTVTFVAS +AA+TTR+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Moc06g09500 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1453.3 bits (3761), Expect = 0.0e+00
Identity = 726/766 (94.78%), Postives = 747/766 (97.52%), Query Frame = 0

Query: 1   MGAIDRLLIAS---LLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSS 60
           MG++ RLL+AS   LLLLLPCVFV+AKRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  TTSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
            TSDSLLYTYT+AYHGFAASLDP+EAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPV LVY+KGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDIST DYI FLCSLDYGIDHVQAI KR NITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKV VKP+KL F+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           ERKRYTVTFVAS +AA+TTR+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Moc06g09500 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 711/765 (92.94%), Postives = 738/765 (96.47%), Query Frame = 0

Query: 1   MGAIDRLLI--ASLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSST 60
           M ++ R LI    LLLLL CVF++AK+TYIV MKHHALPS+YLTHHDWYSA+LQSLSSS+
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  TSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           +SDSLLYTYT+++HGFAA LD EE ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQS RDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNK V LVY+KGSNTSSNMCLPGSL+PAVVRGKVV+CDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDIST DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKV VKP+KL FTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           RKRYTVTFVAS +AAQTTRFGFGSI WSNDQHQVRSPV+FAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Moc06g09500 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 711/765 (92.94%), Postives = 738/765 (96.47%), Query Frame = 0

Query: 1   MGAIDRLLI--ASLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSST 60
           M ++ R LI    LLLLL CVF++AK+TYIV MKHHALPS+YLTHHDWYSA+LQSLSSS+
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  TSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           +SDSLLYTYT+++HGFAA LD EE ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQS RDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNK V LVY+KGSNTSSNMCLPGSL+PAVVRGKVV+CDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDIST DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKV VKP+KL FTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTRL 764
           RKRYTVTFVAS +AAQTTRFGFGSI WSNDQHQVRSPV+FAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Moc06g09500 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 710/762 (93.18%), Postives = 737/762 (96.72%), Query Frame = 0

Query: 1   MGAIDRLLIA-SLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTT 60
           M ++ R LI   LLLLL CVF++AK+TYIV MKHHALPS+YLTHHDWYSA+LQSLSSS++
Sbjct: 1   MDSMPRFLIPFLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSS 60

Query: 61  SDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG 120
           SDSLLYTYT+++HGFAA LD EE ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG
Sbjct: 61  SDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG 120

Query: 121 LWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLC 180
           LWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECE+GPDFSPSLC
Sbjct: 121 LWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSLC 180

Query: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYA 240
           NKKLIGARSFSKGYQMASGGGYFRKPRENQS RDQDGHGTHTASTAAGSHV NASLLGYA
Sbjct: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYA 240

Query: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTI 300
           RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTI
Sbjct: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTI 300

Query: 301 AIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT 360
           AIGAFAAMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT
Sbjct: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT 360

Query: 361 GVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGV 420
           GVSLYSGQGMGNK V LVY+KGSNTSSNMCLPGSL+PAVVRGKVV+CDRGINARVEKGGV
Sbjct: 361 GVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGV 420

Query: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGT 480
           VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGGT
Sbjct: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGT 480

Query: 481 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFN 540
           +LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQFN
Sbjct: 481 ILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFN 540

Query: 541 IMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNP 600
           IMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNP
Sbjct: 541 IMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNP 600

Query: 601 WAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPG 660
           WAHGAGHVDPHKALSPGLLYDIST DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADPG
Sbjct: 601 WAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPG 660

Query: 661 QLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGER 720
           QLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKV VKP+KL FTKVGER
Sbjct: 661 QLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGER 720

Query: 721 KRYTVTFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWT 762
           KRYTVTFVAS +AAQTTRFGFGSI WSNDQHQVRSPV+FAWT
Sbjct: 721 KRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762

BLAST of Moc06g09500 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 550/756 (72.75%), Postives = 638/756 (84.39%), Query Frame = 0

Query: 8   LIASLLLLLPCVFVDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTY 67
           +I + L LL  +   AK+TYI+R+ H   P  +LTHHDWY++ L S S      SLLYTY
Sbjct: 13  IITTFLFLL--LHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTY 72

Query: 68  TAAYHGFAASLDPEEAE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNT 127
           T ++HGF+A LD  EA+ LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+      
Sbjct: 73  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------ 132

Query: 128 QDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGA 187
            DL  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+GECESG DF   LCNKKLIGA
Sbjct: 133 HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGA 192

Query: 188 RSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGM 247
           RSFSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGM
Sbjct: 193 RSFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 252

Query: 248 APQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAA 307
           A +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGAF+A
Sbjct: 253 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSA 312

Query: 308 MEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSG 367
           ME+GVFVSCSAGNSGP RAS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG
Sbjct: 313 MERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG 372

Query: 368 QGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGI 427
            GMG KP+ELVY+KG+++SSN+CLPGSL+ ++VRGK+V+CDRG+NARVEKG VVRDAGG+
Sbjct: 373 VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 432

Query: 428 GMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPS 487
           GMI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YVKS +KPTA+L F GTVL+V+PS
Sbjct: 433 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 492

Query: 488 PVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSM 547
           PVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSM
Sbjct: 493 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 552

Query: 548 SCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGH 607
           SCPHISGLA LLKAAHP+WSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GH
Sbjct: 553 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 612

Query: 608 VDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSF 667
           VDP KALSPGL+YDISTE+YI FLCSLDY +DH+ AIVKRP++ CS+KF+DPGQLNYPSF
Sbjct: 613 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 672

Query: 668 SVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTF 727
           SV+FG KRVVRYTR VTNVGAA SVY+V       V + VKP+KL+F  VGE+KRYTVTF
Sbjct: 673 SVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTF 732

Query: 728 VASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAWTR 763
           V+    + T +  FGSI WSN QH+VRSPVAF+W R
Sbjct: 733 VSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Moc06g09500 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 811.2 bits (2094), Expect = 7.0e-235
Identity = 423/742 (57.01%), Postives = 533/742 (71.83%), Query Frame = 0

Query: 26  TYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAEL 85
           TYIV M    +PS +  H +WY ++L+S+S S     LLYTY  A HGF+  L  EEA+ 
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLYTYENAIHGFSTRLTQEEADS 90

Query: 86  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDL--DQASH-DVIIGVLD 145
           L     V+ V  +  Y LHTTRTP FLGLD         +T DL  +  S+ DV++GVLD
Sbjct: 91  LMTQPGVISVLPEHRYELHTTRTPLFLGLD--------EHTADLFPEAGSYSDVVVGVLD 150

Query: 146 TGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYF 205
           TG+WPESKS+ D G   IPS W+G CE+G +F+ SLCN+KLIGAR F++GY+  S  G  
Sbjct: 151 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE--STMGPI 210

Query: 206 RKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTG 265
            + +E++SPRD DGHGTHT+STAAGS V  ASLLGYA G ARGMAP+ARVA YK CW  G
Sbjct: 211 DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGG 270

Query: 266 CFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGP 325
           CF SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGAFAAME+G+ VSCSAGN+GP
Sbjct: 271 CFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGP 330

Query: 326 NRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVY--DK 385
           + +SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL+ G+ + +K +  +Y  + 
Sbjct: 331 SSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA 390

Query: 386 GSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEEL 445
            + T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+ AGG+GMILANTAA+GEEL
Sbjct: 391 SNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEEL 450

Query: 446 VADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLV 505
           VAD+HLLPA  VG K GD+IR YV +   PTA +S  GTV+ V+PSPVVAAFSSRGPN +
Sbjct: 451 VADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSI 510

Query: 506 TPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKA 565
           TP ILKPD+I PGVNILAAW+ + GPTGL SD R+ +FNI+SGTSMSCPH+SGLAALLK+
Sbjct: 511 TPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKS 570

Query: 566 AHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYD 625
            HP+WSP+AI+SALMTTAY        L D A G  S P+ HGAGHV P  A +PGL+YD
Sbjct: 571 VHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYD 630

Query: 626 ISTEDYIAFLCSLDYGIDHVQAIVKRPNITCS-RKFADPGQLNYPSFSVVFGSKRVVRYT 685
           ++TEDY+ FLC+L+Y    ++++ +R N TC   K      LNYPSF+V        +YT
Sbjct: 631 LTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYT 690

Query: 686 RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFG 745
           R VT+VG AG+     T+  + VK+ V+P  L F +  E+K YTVTF   S+    +   
Sbjct: 691 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-S 750

Query: 746 FGSIAWSNDQHQVRSPVAFAWT 762
           FGSI WS+ +H V SPVA +WT
Sbjct: 751 FGSIEWSDGKHVVGSPVAISWT 757

BLAST of Moc06g09500 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 771.5 bits (1991), Expect = 6.1e-223
Identity = 414/775 (53.42%), Postives = 524/775 (67.61%), Query Frame = 0

Query: 6   RLLIASLLLLLP---CVF------VDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSS 65
           +L ++S+  + P   C F       D   +YIV ++    PS + +H++W+ + L+SL S
Sbjct: 3   KLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPS 62

Query: 66  STTSDSLLYTYTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 125
           S    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D    +HTT TP FLG   
Sbjct: 63  SPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQ 122

Query: 126 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSP 185
           + GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+GECE GPDF  
Sbjct: 123 NSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPA 182

Query: 186 SLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLL 245
           S CN+KLIGAR+F +GY     G      +E++SPRD +GHGTHTASTAAGS VANASL 
Sbjct: 183 SSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLY 242

Query: 246 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP-Y 305
            YARG A GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP Y
Sbjct: 243 QYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEY 302

Query: 306 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGN 365
           + D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    G+
Sbjct: 303 HTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGD 362

Query: 366 GKRFTGVSLYSGQGMGNKPVELVYDKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARV 425
           GK FTG SLY+G+ + +  + LVY    +  S +C PG L  ++V GK+V+CDRG NARV
Sbjct: 363 GKVFTGTSLYAGESLPDSQLSLVY--SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARV 422

Query: 426 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVL 485
           EKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+K+   PTA +
Sbjct: 423 EKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKI 482

Query: 486 SFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDK 545
           SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD D 
Sbjct: 483 SFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDP 542

Query: 546 RKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAG 605
           R+ QFNI+SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D A 
Sbjct: 543 RRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLAT 602

Query: 606 GAFSNPWAHGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNI--TC 665
           G  SN + HGAGHVDP+KAL+PGL+YDI  ++Y+AFLC++ Y    +   ++ P +   C
Sbjct: 603 GKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDAC 662

Query: 666 -SRKFADPGQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYEVATTAPSVVKVVVKP 725
            + K    G LNYPSFSVVF S   VV+Y R+V NVG+   +VYEV   +P+ V++ V P
Sbjct: 663 ETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSP 722

Query: 726 TKLAFTKVGERKRYTVTF---VASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAW 761
           +KLAF+K      Y VTF   V            FGSI W++ +H V+SPVA  W
Sbjct: 723 SKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Moc06g09500 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 748.4 bits (1931), Expect = 5.5e-216
Identity = 391/756 (51.72%), Postives = 504/756 (66.67%), Query Frame = 0

Query: 21  VDAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDS------LLYTYTAAYHGF 80
           +  K+TY++ M   A+P  Y  H  WYS+ + S++   + +       +LYTY  A+HG 
Sbjct: 31  ISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGL 90

Query: 81  AASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASH 140
           AA L  EEAE L + D V+ V  +T Y LHTTR+P FLGL+      E           H
Sbjct: 91  AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ----ESERVWAERVTDH 150

Query: 141 DVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQ 200
           DV++GVLDTGIWPES+SF+DTGM  +P+ WRG CE+G  F    CN+K++GAR F +GY+
Sbjct: 151 DVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYE 210

Query: 201 MASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAA 260
            A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+L G+A G ARGMA +ARVAA
Sbjct: 211 AAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAA 270

Query: 261 YKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVS 320
           YK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y RD+++I  F AME GVFVS
Sbjct: 271 YKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVS 330

Query: 321 CSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGM--GNK 380
           CSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G  + F GVSLY G+ +   NK
Sbjct: 331 CSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNK 390

Query: 381 PVELVYDKGSNTSS----NMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGM 440
              LVY  G N SS    + CL G+L+   V GK+VICDRG+  RV+KG VV+ AGGIGM
Sbjct: 391 QYPLVY-LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGM 450

Query: 441 ILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPV 500
           +L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K TA L   GT + ++PSPV
Sbjct: 451 VLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPV 510

Query: 501 VAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSC 560
           VAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD R+ +FNI+SGTSMSC
Sbjct: 511 VAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSC 570

Query: 561 PHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVD 620
           PH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L DA+G A S+P+ HGAGH+D
Sbjct: 571 PHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHID 630

Query: 621 PHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFA-DPGQLNYPSFS 680
           P +A  PGL+YDI  ++Y  FLC+ D     ++   K  N TC    A +PG LNYP+ S
Sbjct: 631 PLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAIS 690

Query: 681 VVFGSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTV 740
            +F      + +   R VTNVG   S Y+V+ +      V V+P  L FT   ++  YTV
Sbjct: 691 ALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTV 750

Query: 741 TFVASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAW 761
           TF       +  R  FG + W +  H+VRSPV   W
Sbjct: 751 TF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Moc06g09500 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 728.0 bits (1878), Expect = 7.8e-210
Identity = 386/762 (50.66%), Postives = 511/762 (67.06%), Query Frame = 0

Query: 12  LLLLLPCVFV-----DAKRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYT 71
           LLL L   F+      A +T+I R+   ++PS + TH+ WYS      S       +++ 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIVHV 67

Query: 72  YTAAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNT 131
           Y   +HGF+A + P+EA+ LR   +VL V+ED    LHTTR+P FLGL +  GLW     
Sbjct: 68  YHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW----- 127

Query: 132 QDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGA 191
            + D  S DVIIGV DTGIWPE +SF D  +  IP RWRG CESG  FSP  CN+K+IGA
Sbjct: 128 SESDYGS-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGA 187

Query: 192 RSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGM 251
           R F+KG Q A  GG   K  E  SPRD DGHGTHT+STAAG H   AS+ GYA G+A+G+
Sbjct: 188 RFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 247

Query: 252 APQARVAAYKTCW-PTGCFGSDILAGMDRAISDGVDVLSLSLGGG---SAPYYRDTIAIG 311
           AP+AR+AAYK CW  +GC  SDILA  D A+ DGVDV+S+S+GGG   ++PYY D IAIG
Sbjct: 248 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 307

Query: 312 AFAAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVS 371
           ++ A  KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R  GVS
Sbjct: 308 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 367

Query: 372 LYSGQGMGNKPVELVY-DKGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVR 431
           LY+G  +  +   +VY  K   +S+++C+  +L+P  VRGK+VICDRG + RV KG VV+
Sbjct: 368 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 427

Query: 432 DAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVL 491
            AGG+GMILAN A++GE LV D+HL+PA AVG   GD I+ Y  S   P A + F GT++
Sbjct: 428 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 487

Query: 492 NVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIM 551
            ++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL SD RKT+FNI+
Sbjct: 488 GIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 547

Query: 552 SGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWA 611
           SGTSM+CPH+SG AALLK+AHP WSP+ I+SA+MTT    DN+N SL D + G  + P+ 
Sbjct: 548 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYD 607

Query: 612 HGAGHVDPHKALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQL 671
           +G+GH++  +A++PGL+YDI+ +DYI FLCS+ YG   +Q I + P    + +   PG L
Sbjct: 608 YGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNL 667

Query: 672 NYPSFSVVFGSKR--VVRYT--RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVG 731
           NYPS + VF + R  +V  T  R  TNVG A +VY     +P  V V VKP +L FT   
Sbjct: 668 NYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAV 727

Query: 732 ERKRYTVTFVASSNAAQTTRFG--FGSIAW-SNDQHQVRSPV 757
           +R+ Y VT   ++        G  FGS+ W    +H VRSP+
Sbjct: 728 KRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022987083.10.0e+0094.91subtilisin-like protease SBT1.8 [Cucurbita maxima][more]
KAG6590040.10.0e+0094.90Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022960792.10.0e+0094.78subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-li... [more]
XP_023516159.10.0e+0094.64subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo][more]
XP_038879224.10.0e+0094.63subtilisin-like protease SBT1.8 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9ZUF60.0e+0072.75Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
O653519.8e-23457.01Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9LVJ18.6e-22253.42Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... [more]
Q9FLI47.8e-21551.72Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
O496071.1e-20850.66Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JIF30.0e+0094.91subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3... [more]
A0A6J1H8F10.0e+0094.78subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE... [more]
A0A5A7UKV30.0e+0092.94Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BQE80.0e+0092.94subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=... [more]
A0A5D3CDV90.0e+0093.18Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
AT2G05920.10.0e+0072.75Subtilase family protein [more]
AT5G67360.17.0e-23557.01Subtilase family protein [more]
AT3G14067.16.1e-22353.42Subtilase family protein [more]
AT5G51750.15.5e-21651.72subtilase 1.3 [more]
AT4G34980.17.8e-21050.66subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 133..152
score: 29.87
coord: 213..226
score: 49.97
coord: 542..558
score: 56.45
NoneNo IPR availableGENE3D3.50.30.30coord: 343..481
e-value: 9.9E-187
score: 623.3
NoneNo IPR availableGENE3D2.60.40.2310coord: 630..760
e-value: 5.6E-36
score: 125.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 200..224
NoneNo IPR availablePANTHERPTHR10795:SF653CUCUMISIN-RELATEDcoord: 16..760
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 117..613
score: 28.148706
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 349..472
e-value: 2.3672E-39
score: 139.471
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 26..102
IPR003137PA domainPFAMPF02225PAcoord: 375..459
e-value: 5.2E-10
score: 39.3
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 134..584
e-value: 4.9E-50
score: 170.9
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 26..104
e-value: 8.3E-15
score: 55.2
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 135..626
e-value: 9.9E-187
score: 623.3
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 103..617
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 660..757
e-value: 1.0E-26
score: 93.0
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 21..104
e-value: 8.2E-19
score: 69.8
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 16..760
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 543..553
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 101..579
e-value: 2.93236E-145
score: 426.244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g09500.1Moc06g09500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity