Moc06g09390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g09390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	(1->3)-beta-glucan endohydrolase
Location	chr6: 6856555 .. 6857586 (-)
RNA-Seq Expression	Moc06g09390
Synteny	Moc06g09390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAGCTTGGCCATGTAATAATCGTCGTCTTCTTCATGGCCGCCACGTCAGCCGTGGGAGCACACGACGTTCTTCTCGGCGCGTACTACGGTCTCGCCGGGAACAACCTGCCGCCGCCGTGGGATGTGGTGCAACTTTGCAGAAAAAACAACATCCGCCGCGTTCGATTAGACGAACCAAACCTCGACGTTTTTCAAGCGTTCCGCGGCGCAGAAATCGACCTCTCCTTCGGCGTACCCAACAACATGTTAATCAACATGAAAACCAACAGCACCGCCGTGGAAGAGTGGTTCAACACCTACGTGGAGCCGTTCATCGGCGATTTCACCATCAATTACATCGTCGTCGGCGACAAGGCCATCCCGGGCCTCGAAGACTGCATATTACCCGTCATGAAATCCCTCCAAGATCTCCTCAACTCCCACTACCTCGGACAAGTCAAACTCACGACATTGGTCGGGTACAACTCGGCGTTGGTCGTCGACGGCGGCCTCCCGTCCAACGCCACGTTCAATCCAGTCGCCAGCGACAACATCAAAGGCATCCTTAAATTCCTGTTCGTCGAAGGCTCGCCGCTGATGGTCAGCGTGTACCCATTTCGAAAATATGCATACAACAATGACAAAGCTGTAAGTTTGGATTACGCGATGTTTCGTTCGAAAGTGGCGGTGGTTCGTGACGGTGAGGTGAGCTATTACAACTTGTTCGATGCAATGGTGGATGCATTTTACACGGCAATCGAGAAGCAAGCCGTGGGAGATGTGGCGGTGGCGGTGGGGGAAACTGGGTGGCCGACGTGTGATCATGGTTATGCTGCAACCCCGGAATATGCTGCATTGTATAACCGGAACTTTAAGAGACATATTTGCTCTGGAAAGGGAACTCCGAAGAAGCAGAATATTTATATAGAAGGATTTATTCAGAGTATATTCGATGAGAATGAAAAGCCTGAGGGAGACTCGAGGTGTTATGGAATGTTTCATGTCGATAAAATACCTGTTTATCCTAATGTGTTTTGTATTGGGTAA

mRNA sequence

ATGGCAAAGCTTGGCCATGTAATAATCGTCGTCTTCTTCATGGCCGCCACGTCAGCCGTGGGAGCACACGACGTTCTTCTCGGCGCGTACTACGGTCTCGCCGGGAACAACCTGCCGCCGCCGTGGGATGTGGTGCAACTTTGCAGAAAAAACAACATCCGCCGCGTTCGATTAGACGAACCAAACCTCGACGTTTTTCAAGCGTTCCGCGGCGCAGAAATCGACCTCTCCTTCGGCGTACCCAACAACATGTTAATCAACATGAAAACCAACAGCACCGCCGTGGAAGAGTGGTTCAACACCTACGTGGAGCCGTTCATCGGCGATTTCACCATCAATTACATCGTCGTCGGCGACAAGGCCATCCCGGGCCTCGAAGACTGCATATTACCCGTCATGAAATCCCTCCAAGATCTCCTCAACTCCCACTACCTCGGACAAGTCAAACTCACGACATTGGTCGGGTACAACTCGGCGTTGGTCGTCGACGGCGGCCTCCCGTCCAACGCCACGTTCAATCCAGTCGCCAGCGACAACATCAAAGGCATCCTTAAATTCCTGTTCGTCGAAGGCTCGCCGCTGATGGTCAGCGTGTACCCATTTCGAAAATATGCATACAACAATGACAAAGCTGTAAGTTTGGATTACGCGATGTTTCGTTCGAAAGTGGCGGTGGTTCGTGACGGTGAGGTGAGCTATTACAACTTGTTCGATGCAATGGTGGATGCATTTTACACGGCAATCGAGAAGCAAGCCGTGGGAGATGTGGCGGTGGCGGTGGGGGAAACTGGGTGGCCGACGTGTGATCATGGTTATGCTGCAACCCCGGAATATGCTGCATTGTATAACCGGAACTTTAAGAGACATATTTGCTCTGGAAAGGGAACTCCGAAGAAGCAGAATATTTATATAGAAGGATTTATTCAGAGTATATTCGATGAGAATGAAAAGCCTGAGGGAGACTCGAGGTGTTATGGAATGTTTCATGTCGATAAAATACCTGTTTATCCTAATGTGTTTTGTATTGGGTAA

Coding sequence (CDS)

ATGGCAAAGCTTGGCCATGTAATAATCGTCGTCTTCTTCATGGCCGCCACGTCAGCCGTGGGAGCACACGACGTTCTTCTCGGCGCGTACTACGGTCTCGCCGGGAACAACCTGCCGCCGCCGTGGGATGTGGTGCAACTTTGCAGAAAAAACAACATCCGCCGCGTTCGATTAGACGAACCAAACCTCGACGTTTTTCAAGCGTTCCGCGGCGCAGAAATCGACCTCTCCTTCGGCGTACCCAACAACATGTTAATCAACATGAAAACCAACAGCACCGCCGTGGAAGAGTGGTTCAACACCTACGTGGAGCCGTTCATCGGCGATTTCACCATCAATTACATCGTCGTCGGCGACAAGGCCATCCCGGGCCTCGAAGACTGCATATTACCCGTCATGAAATCCCTCCAAGATCTCCTCAACTCCCACTACCTCGGACAAGTCAAACTCACGACATTGGTCGGGTACAACTCGGCGTTGGTCGTCGACGGCGGCCTCCCGTCCAACGCCACGTTCAATCCAGTCGCCAGCGACAACATCAAAGGCATCCTTAAATTCCTGTTCGTCGAAGGCTCGCCGCTGATGGTCAGCGTGTACCCATTTCGAAAATATGCATACAACAATGACAAAGCTGTAAGTTTGGATTACGCGATGTTTCGTTCGAAAGTGGCGGTGGTTCGTGACGGTGAGGTGAGCTATTACAACTTGTTCGATGCAATGGTGGATGCATTTTACACGGCAATCGAGAAGCAAGCCGTGGGAGATGTGGCGGTGGCGGTGGGGGAAACTGGGTGGCCGACGTGTGATCATGGTTATGCTGCAACCCCGGAATATGCTGCATTGTATAACCGGAACTTTAAGAGACATATTTGCTCTGGAAAGGGAACTCCGAAGAAGCAGAATATTTATATAGAAGGATTTATTCAGAGTATATTCGATGAGAATGAAAAGCCTGAGGGAGACTCGAGGTGTTATGGAATGTTTCATGTCGATAAAATACCTGTTTATCCTAATGTGTTTTGTATTGGGTAA

Protein sequence

MAKLGHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYPNVFCIG

Homology

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BLAST of Moc06g09390 vs. NCBI nr
Match: XP_022136187.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia])

HSP 1 Score: 483.8 bits (1244), Expect = 1.2e-132
Identity = 235/337 (69.73%), Postives = 281/337 (83.38%), Query Frame = 0


Query: 1   MAKLGHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDE 60
           MAKL H  I+VFFMA+ SAV A+DVLLG YYGL G+N+PPPW VVQLC K NIRR+RLDE
Sbjct: 1   MAKLAH--IIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDE 60

Query: 61  PNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDK 120
           PNL+VF AF GA ID+SFGVPN+MLINMK N +AV EWFNTYVEP+IGDF INYI+VG+K
Sbjct: 61  PNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNK 120

Query: 121 AIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNI 180
           AIPGL+DCILPVMKSLQ+LLN+ YLGQVKLTTLVGYN+AL V    PS+  F+P ASDNI
Sbjct: 121 AIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDP-PSSGAFDPNASDNI 180

Query: 181 KGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAM 240
           + IL+FL  EGSPLMVSV P+ KYAY+N +A+SL+YA F +K  VV DG +SY NLFDAM
Sbjct: 181 REILRFLLREGSPLMVSVCPYTKYAYSN-RAISLNYATFGAKTPVVHDGGLSYDNLFDAM 240

Query: 241 VDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQ 300
           VD FY A++K  VGDV VAVGETGWPTC +G  ATP+YA+ YN+NFK HI SG+GTPK+ 
Sbjct: 241 VDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRP 300

Query: 301 NIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYP 338
           N+ I GFI+S+F+EN+ PEG+S+CYG+F+VD  P+YP
Sbjct: 301 NVLIRGFIKSMFNENKMPEGESQCYGIFNVDSTPLYP 333

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BLAST of Moc06g09390 vs. NCBI nr
Match: XP_038880473.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 448.7 bits (1153), Expect = 4.4e-122
Identity = 215/338 (63.61%), Postives = 269/338 (79.59%), Query Frame = 0


Query: 1   MAKLGHVIIVV--FFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRL 60
           MAKL  +I ++    MAAT  V A++VLLG   GL+G+NLPPPW +VQLC+K +IRR+RL
Sbjct: 1   MAKLAQIIFLMASIIMAAT-IVRAYNVLLGVCLGLSGDNLPPPWKMVQLCKKYSIRRIRL 60

Query: 61  DEPNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVG 120
           DEPN+DV  AFRGAEIDLSFG+PN+ML +M TN   VEEWFNTY++PF+ +F INY++VG
Sbjct: 61  DEPNIDVLNAFRGAEIDLSFGIPNDMLTDMATNKALVEEWFNTYIKPFVNEFKINYVIVG 120

Query: 121 DKAIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASD 180
           DKAIPGL+ CILPVM S QDLLN++Y+GQVKLTTLVGYN+AL      PS+  F+P  S+
Sbjct: 121 DKAIPGLDICILPVMMSFQDLLNANYIGQVKLTTLVGYNAALAYKDP-PSSGAFHPTISE 180

Query: 181 NIKGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFD 240
           N++GILKFL  EGSPLMVSV+P+RKY +  D  +SL+YA+F     VVRDGE++YYNLFD
Sbjct: 181 NMRGILKFLSEEGSPLMVSVFPYRKYMF--DGGISLNYAIFNETEPVVRDGELNYYNLFD 240

Query: 241 AMVDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPK 300
           AMV+A Y AI+K+AVGDV V VGETGWPTC     ATP  A  YN+ F  HICSGKGTP+
Sbjct: 241 AMVEAVYAAIDKEAVGDVTVVVGETGWPTCGSDDDATPAIAEEYNKKFVSHICSGKGTPR 300

Query: 301 KQNIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVY 337
           K N+++EGFIQSIF+E+EKP+GDS+CYGMF ++  P+Y
Sbjct: 301 KPNVFLEGFIQSIFNEDEKPQGDSQCYGMFDINMNPIY 334

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BLAST of Moc06g09390 vs. NCBI nr
Match: KAG6590489.1 (putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 444.5 bits (1142), Expect = 8.3e-121
Identity = 220/333 (66.07%), Postives = 261/333 (78.38%), Query Frame = 0


Query: 9   IVVFFMAATS-AVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQ 68
           ++  FMA  S  VGA+DVLLGAYYGL G+NLP P  VVQLCRK NI RVRL+ PNLDV +
Sbjct: 1   MIGLFMADMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIE 60

Query: 69  AFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPGLED 128
            FRG EIDLSFGVPN+ML NM TN T VEEWFNTYVEP+  D TINYIVVGDKAIPGL+D
Sbjct: 61  GFRGTEIDLSFGVPNDMLPNMATNKTLVEEWFNTYVEPYTNDCTINYIVVGDKAIPGLDD 120

Query: 129 CILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFL 188
            ILPVMKSLQDLLN+HY G+VKLTTLVG+N+  V +   PS+  FNP   +N++GIL+FL
Sbjct: 121 NILPVMKSLQDLLNAHYFGEVKLTTLVGFNALAVTNP--PSSGAFNPAIVENMRGILEFL 180

Query: 189 FVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTA 248
             EGSPLMVSVY + +Y Y  +  +SL YA    +  +VRDG +SY NLFD +VDAFY A
Sbjct: 181 RGEGSPLMVSVYTYDQYVYGGN--ISLGYATLSEQNTLVRDGMLSYNNLFDEIVDAFYAA 240

Query: 249 IEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGF 308
           ++K+ VGDVA+  GETGWP+C +  A TP  AA YNRNFK HI SGKGTP+K+NIYIEGF
Sbjct: 241 MDKENVGDVAIVFGETGWPSCGNYNATTPLLAAEYNRNFKNHISSGKGTPRKENIYIEGF 300

Query: 309 IQSIFDENEKPEGDSRCYGMFHVDKIPVYPNVF 341
           I+SIF+EN+KPEG+SRCYGMF+VD  P+Y  VF
Sbjct: 301 IRSIFNENQKPEGESRCYGMFNVDSTPIYSPVF 329

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BLAST of Moc06g09390 vs. NCBI nr
Match: XP_038880685.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 443.0 bits (1138), Expect = 2.4e-120
Identity = 222/340 (65.29%), Postives = 264/340 (77.65%), Query Frame = 0


Query: 1   MAKLGHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDE 60
           MAKL H IIV+F +  +S VGA+DVLLGAYY LAGNNLPPPW+VVQLC+K NIRRVRL E
Sbjct: 1   MAKLSH-IIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYE 60

Query: 61  PNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDK 120
           PNL V ++FRG  ID+SFGVPNN++ NM TN TAVEEWF  YV PFIGDFTINYIVVGDK
Sbjct: 61  PNLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDK 120

Query: 121 AIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNI 180
            IPGL+  IL VM+SLQ LLN+  LGQVKLTTLV   +AL      PS+  F+P  + N+
Sbjct: 121 VIPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSI-TALSAQSP-PSSGAFDPTIAANM 180

Query: 181 KGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAM 240
           +GIL FL+ +GSPLMVS+YP + Y Y+ +  +SL YA F  +   +RDG++SY NLFD M
Sbjct: 181 RGILHFLWEQGSPLMVSLYPHKTYFYSGN--MSLGYATFAEENFSIRDGDLSYNNLFDEM 240

Query: 241 VDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQ 300
           +DAFY AI+K+ VGDVA+AVGETGWPTC H    T   AA YNRNFK HI SGKGTP K 
Sbjct: 241 MDAFYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKS 300

Query: 301 NIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYPNVF 341
           NIYI+GFI+SIF+ENE+PEG+S+CYGMFHVD  P+Y  VF
Sbjct: 301 NIYIQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 335

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BLAST of Moc06g09390 vs. NCBI nr
Match: XP_022968751.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima])

HSP 1 Score: 442.6 bits (1137), Expect = 3.1e-120
Identity = 219/340 (64.41%), Postives = 263/340 (77.35%), Query Frame = 0


Query: 1   MAKLGHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDE 60
           M K+ H +I +F       VGA+DVLLGAYYGL G+NL  P  VVQLCRK NI RVRL+ 
Sbjct: 1   MVKISH-MIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEV 60

Query: 61  PNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDK 120
            N+DV +AFRG EIDLS GVPN+ML NM TN T VEEWFNTYVEP++ D+ INYIVVGDK
Sbjct: 61  SNIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDK 120

Query: 121 AIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNI 180
           AIPGL+D ILPVMKSLQDLLN+HY G+ KLTTLVG+N+  V +   PS+  FNP   +N+
Sbjct: 121 AIPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFNALAVTNP--PSSGAFNPAIVENM 180

Query: 181 KGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAM 240
           +GIL+FL  EGSPLMVSVY + +Y Y  ++  SL YA    + ++VRDG +SY NLFD M
Sbjct: 181 RGILEFLRGEGSPLMVSVYTYDQYVYGGNR--SLGYATLSEQNSLVRDGMLSYNNLFDEM 240

Query: 241 VDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQ 300
           VDAFY AI+K+ VGDVA+ +GETGWPTC +  A TP  AA YNRNFK HI SGKGTP+K+
Sbjct: 241 VDAFYAAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKE 300

Query: 301 NIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYPNVF 341
           NIYIEGFI+SIF+EN+KPEG+SRCYGMF+VD  P+Y  VF
Sbjct: 301 NIYIEGFIRSIFNENQKPEGESRCYGMFNVDSTPIYSPVF 335

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BLAST of Moc06g09390 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-61
Identity = 128/333 (38.44%), Postives = 205/333 (61.56%), Query Frame = 0


Query: 13  FMAATSAVGAHDVL--LGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQAFR 72
           F+ A +++G  +    +G  YGL G+NLP P  V+ L +  +I ++R+ +PN +V  A R
Sbjct: 27  FVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALR 86

Query: 73  G-AEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPG-LEDC 132
           G  +I ++ GV +  L  +  +  AV+ WF T +EP++ D  I +I VG++ IPG +   
Sbjct: 87  GHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQ 146

Query: 133 ILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFLF 192
           +LPVM+SL +L+ S  L  + ++T+V   +  +     PS   F   A + +  +LK L 
Sbjct: 147 VLPVMQSLTNLVKSRNL-PISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLS 206

Query: 193 VEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTAI 252
              SP++V +YP+  YA ++  ++ LDYA F ++  VV+DG + Y N+FDA+ DAF  A+
Sbjct: 207 QTNSPILVKIYPYFSYA-SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAM 266

Query: 253 EKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGFI 312
           EK+ V D+ + V ETGWP+  +G   TP+ A  YNRNF +HI SGKGTPK+ N  I+GF+
Sbjct: 267 EKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFL 326

Query: 313 QSIFDENEKPEGDSRCYGMFHV-DKIPVYPNVF 341
            + F+EN+KP G  + +G+++  D  P+Y N+F
Sbjct: 327 FATFNENQKPVGTEQNFGLYNPNDMKPIY-NLF 354

[Close]

BLAST of Moc06g09390 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.8e-60
Identity = 122/314 (38.85%), Postives = 195/314 (62.10%), Query Frame = 0


Query: 27  LGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQAFRG-AEIDLSFGVPNNML 86
           +G  YGL G+NLP P +V+ L +   I R+R+ +PN +V  A RG  +I+++ GV +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 87  INMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPG-LEDCILPVMKSLQDLLNSHY 146
             +  +  AV+ WF   +E ++ D  I +I VG++ IPG +   +LPVM+SL +L+ S  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 147 LGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFLFVEGSPLMVSVYPFRKY 206
           L  + ++T+V  ++  +     PS   F   A + +  +LK L    +P++V++YP+  +
Sbjct: 154 L-PISISTVVAMSN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY--F 213

Query: 207 AYNNDKA-VSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTAIEKQAVGDVAVAVGET 266
           AY +D A + LDYA F +K  VV+DG + Y N+FDA+ DAF  A+EK+ V ++ + V ET
Sbjct: 214 AYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSET 273

Query: 267 GWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGFIQSIFDENEKPEGDSR 326
           GWP+  +G   TP  A+ YNRNF +HI SGKGTPK+ N  + GF+ + F+EN+KP G  +
Sbjct: 274 GWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQ 333

Query: 327 CYGMFH-VDKIPVY 337
            +G+++  D  P+Y
Sbjct: 334 NFGLYNPSDMKPIY 342

[Close]

BLAST of Moc06g09390 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 220.3 bits (560), Expect = 3.3e-56
Identity = 125/335 (37.31%), Postives = 194/335 (57.91%), Query Frame = 0


Query: 5   GHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLD 64
           G  ++V   +A  + V +    +G  YG+ GNNLPPP +VV L + NNI R+RL +PN  
Sbjct: 11  GFALLVSVLVAVPTRVQS----IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQA 70

Query: 65  VFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPG 124
             QA R + I +   VP + + ++ +N +A  +W    V  +    +  YI VG++ IPG
Sbjct: 71  ALQALRNSNIQVLLDVPRSDVQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPG 130

Query: 125 --LEDCILPVMKSLQDLLNSHYL-GQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIK 184
             L   ILP M+++ + L+S  L  Q+K++T V  ++ ++     PS   F+  A   + 
Sbjct: 131 SDLAQYILPAMRNIYNALSSAGLQNQIKVSTAV--DTGVLGTSYPPSAGAFSSAAQAYLS 190

Query: 185 GILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMV 244
            I++FL   G+PL+V+VYP+  Y   N   +SL YA+F +   VV+DG  SY NLFDA+V
Sbjct: 191 PIVQFLASNGAPLLVNVYPYFSYT-GNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIV 250

Query: 245 DAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQN 304
           DA + A+E+    +VAV V E+GWP+   G  A+   A  YN+N  RH+  G GTP++  
Sbjct: 251 DAVFAALERVGGANVAVVVSESGWPSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPG 310

Query: 305 IYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVY 337
             IE +I  +F+EN+K  G  + +G+F+ +K PVY
Sbjct: 311 KEIEAYIFEMFNENQKAGGIEQNFGLFYPNKQPVY 336

[Close]

BLAST of Moc06g09390 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 209.9 bits (533), Expect = 4.5e-53
Identity = 122/344 (35.47%), Postives = 192/344 (55.81%), Query Frame = 0


Query: 3   KLGHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPN 62
           ++  +I++   +++T  VGA  V  G  YG+ GNNLPP   VVQL +  NIRR+RL +PN
Sbjct: 11  QMAAIILLGLLVSSTEIVGAQSV--GVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPN 70

Query: 63  LDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAI 122
               QA RG+ I++  GVPN+ L N+  N +    W    V  F       YI VG++  
Sbjct: 71  QAALQALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVS 130

Query: 123 P-----GLEDCILPVMKSLQDLLNSHYL-GQVKLTTLVGYNSALVVDGGLPSNATFNPVA 182
           P      L   +LP M+++++ ++S  L   +K+++ V  +  L+ +   PS  +F    
Sbjct: 131 PVTGTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSV--DMTLIGNSFPPSQGSFRNDV 190

Query: 183 SDNIKGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNL 242
              I  I+ F+    SPL+V++YP+  YA  N + +SL YA+F +   VV+DG + Y NL
Sbjct: 191 RSFIDPIIGFVRRINSPLLVNIYPYFSYA-GNPRDISLPYALFTAPNVVVQDGSLGYRNL 250

Query: 243 FDAMVDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGT 302
           FDAM DA Y A+ +   G + + V E+GWP+    +AAT   AA Y +N  +H+   +G+
Sbjct: 251 FDAMSDAVYAALSRAGGGSIEIVVSESGWPSAG-AFAATTNNAATYYKNLIQHV--KRGS 310

Query: 303 PKKQNIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYPNVF 341
           P++ N  IE ++ ++FDEN K     + +G+F  +K P YP  F
Sbjct: 311 PRRPNKVIETYLFAMFDENNKNPELEKHFGLFSPNKQPKYPLSF 346

[Close]

BLAST of Moc06g09390 vs. ExPASy Swiss-Prot
Match: P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 3.2e-51
Identity = 124/337 (36.80%), Postives = 191/337 (56.68%), Query Frame = 0


Query: 7   VIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVF 66
           V++++FF AA  +VG  D  +G  YG+ GNNLPP  +V+ L +K+NI R+R+ +PN  V 
Sbjct: 20  VMLLLFFFAA--SVGITDAQVGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPNRAVL 79

Query: 67  QAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIP--- 126
           +A RG+ I+L  GVPN+ L ++ TN +  + W    V  F       YI VG++  P   
Sbjct: 80  EALRGSNIELILGVPNSDLQSL-TNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEISPVNR 139

Query: 127 ---GLEDCILPVMKSLQDLLNSHYL-GQVKLTTLVGYNSALVVDGGLPSNATFNPVASDN 186
               L   +LP M+++ D + S  L  Q+K++T +  +  LV +   PS   F       
Sbjct: 140 GTAWLAQFVLPAMRNIHDAIRSAGLQDQIKVSTAI--DLTLVGNSYPPSAGAFRDDVRSY 199

Query: 187 IKGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDA 246
           +  I+ FL    SPL+ ++YP+  YAY N + +SL YA+F S   VV DG+  Y NLFDA
Sbjct: 200 LDPIIGFLSSIRSPLLANIYPYFTYAY-NPRDISLPYALFTSPSVVVWDGQRGYKNLFDA 259

Query: 247 MVDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKK 306
            +DA Y+A+E+ + G + V V E+GWP+    +AAT +    Y  N  +H+    GTPK+
Sbjct: 260 TLDALYSALERASGGSLEVVVSESGWPSAG-AFAATFDNGRTYLSNLIQHVKG--GTPKR 319

Query: 307 QNIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVY 337
            N  IE ++ ++FDEN+K     + +G+F  +K   Y
Sbjct: 320 PNRAIETYLFAMFDENKKQPEVEKHFGLFFPNKWQKY 347

[Close]

BLAST of Moc06g09390 vs. ExPASy TrEMBL
Match: A0A6J1C4V7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.0e-133
Identity = 235/337 (69.73%), Postives = 281/337 (83.38%), Query Frame = 0


Query: 1   MAKLGHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDE 60
           MAKL H  I+VFFMA+ SAV A+DVLLG YYGL G+N+PPPW VVQLC K NIRR+RLDE
Sbjct: 1   MAKLAH--IIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDE 60

Query: 61  PNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDK 120
           PNL+VF AF GA ID+SFGVPN+MLINMK N +AV EWFNTYVEP+IGDF INYI+VG+K
Sbjct: 61  PNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNK 120

Query: 121 AIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNI 180
           AIPGL+DCILPVMKSLQ+LLN+ YLGQVKLTTLVGYN+AL V    PS+  F+P ASDNI
Sbjct: 121 AIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDP-PSSGAFDPNASDNI 180

Query: 181 KGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAM 240
           + IL+FL  EGSPLMVSV P+ KYAY+N +A+SL+YA F +K  VV DG +SY NLFDAM
Sbjct: 181 REILRFLLREGSPLMVSVCPYTKYAYSN-RAISLNYATFGAKTPVVHDGGLSYDNLFDAM 240

Query: 241 VDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQ 300
           VD FY A++K  VGDV VAVGETGWPTC +G  ATP+YA+ YN+NFK HI SG+GTPK+ 
Sbjct: 241 VDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRP 300

Query: 301 NIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYP 338
           N+ I GFI+S+F+EN+ PEG+S+CYG+F+VD  P+YP
Sbjct: 301 NVLIRGFIKSMFNENKMPEGESQCYGIFNVDSTPLYP 333

[Close]

BLAST of Moc06g09390 vs. ExPASy TrEMBL
Match: A0A6J1HVR6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.5e-120
Identity = 219/340 (64.41%), Postives = 263/340 (77.35%), Query Frame = 0


Query: 1   MAKLGHVIIVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDE 60
           M K+ H +I +F       VGA+DVLLGAYYGL G+NL  P  VVQLCRK NI RVRL+ 
Sbjct: 1   MVKISH-MIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEV 60

Query: 61  PNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDK 120
            N+DV +AFRG EIDLS GVPN+ML NM TN T VEEWFNTYVEP++ D+ INYIVVGDK
Sbjct: 61  SNIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDK 120

Query: 121 AIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNI 180
           AIPGL+D ILPVMKSLQDLLN+HY G+ KLTTLVG+N+  V +   PS+  FNP   +N+
Sbjct: 121 AIPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFNALAVTNP--PSSGAFNPAIVENM 180

Query: 181 KGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAM 240
           +GIL+FL  EGSPLMVSVY + +Y Y  ++  SL YA    + ++VRDG +SY NLFD M
Sbjct: 181 RGILEFLRGEGSPLMVSVYTYDQYVYGGNR--SLGYATLSEQNSLVRDGMLSYNNLFDEM 240

Query: 241 VDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQ 300
           VDAFY AI+K+ VGDVA+ +GETGWPTC +  A TP  AA YNRNFK HI SGKGTP+K+
Sbjct: 241 VDAFYAAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKE 300

Query: 301 NIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYPNVF 341
           NIYIEGFI+SIF+EN+KPEG+SRCYGMF+VD  P+Y  VF
Sbjct: 301 NIYIEGFIRSIFNENQKPEGESRCYGMFNVDSTPIYSPVF 335

[Close]

BLAST of Moc06g09390 vs. ExPASy TrEMBL
Match: A0A1S3BQX5 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 4.6e-117
Identity = 214/341 (62.76%), Postives = 261/341 (76.54%), Query Frame = 0


Query: 1   MAKLGHVIIVVFFMAA----TSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRV 60
           MAKL  V I+VF MA+    TS V AHDVLLGAYYGL+GNNLPPPW VV LC K NIRR+
Sbjct: 1   MAKL--VQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRI 60

Query: 61  RLDEPNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIV 120
           RLD P +DV  AFRG+ IDLSF VPN+ML +M TN   VEEWFNTY++PF+ DF INYI+
Sbjct: 61  RLDIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYII 120

Query: 121 VGDKAIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVA 180
           VGDKA+PGL++ ILPVM SLQDLLN++Y GQVKLTTLVGYN+ALV     PS+  FNP  
Sbjct: 121 VGDKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDP-PSSGVFNPTV 180

Query: 181 SDNIKGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNL 240
            +N++GILKFL  EGS LMVSV+P R+Y  N    +SL+YA  +    VV DGE+ Y NL
Sbjct: 181 RENMRGILKFLAEEGSALMVSVFPHRQYKSNG--GMSLNYAALKETKPVVEDGELKYSNL 240

Query: 241 FDAMVDAFYTAIEKQAVGDVAVAVGETGWPTC-DHGYAATPEYAALYNRNFKRHICSGKG 300
           FDAM+DAFY AIEK+ +G+V + VGETGWPTC D+    T + A  YN  FK HI SGKG
Sbjct: 241 FDAMMDAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKG 300

Query: 301 TPKKQNIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVY 337
           TP+K N+Y+EGFIQS+F+EN+KPEG+S+C+G+F V+  P+Y
Sbjct: 301 TPRKPNVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIY 336

[Close]

BLAST of Moc06g09390 vs. ExPASy TrEMBL
Match: A0A5A7UKS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001680 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 6.6e-116
Identity = 204/321 (63.55%), Postives = 249/321 (77.57%), Query Frame = 0


Query: 17  TSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQAFRGAEIDL 76
           TS V AHDVLLGAYYGL+GNNLPPPW VV LC K NIRR+RLD P +DV  AFRG+ IDL
Sbjct: 8   TSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRGSNIDL 67

Query: 77  SFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPGLEDCILPVMKSL 136
           SF VPN+ML +M TN   VEEWFNTY++PF+ DF INYI+VGDKA+PGL++ ILPVM SL
Sbjct: 68  SFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSL 127

Query: 137 QDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFLFVEGSPLMV 196
           QDLLN++Y GQVKLTTLVGYN+ALV     PS+  FNP   +N++GILKFL  EGS LMV
Sbjct: 128 QDLLNANYFGQVKLTTLVGYNAALVSQDP-PSSGVFNPTVRENMRGILKFLAEEGSALMV 187

Query: 197 SVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTAIEKQAVGDV 256
           SV+P R+Y  N    +SL+YA  +    VV DGE+ Y NLFDAM+DAFY AIEK+ +G+V
Sbjct: 188 SVFPHRQYKSNG--GMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEV 247

Query: 257 AVAVGETGWPTC-DHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGFIQSIFDEN 316
            + VGETGWPTC D+    T + A  YN  FK HI SGKGTP+K N+Y+EGFIQS+F+EN
Sbjct: 248 TLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNEN 307

Query: 317 EKPEGDSRCYGMFHVDKIPVY 337
           +KPEG+S+C+G+F V+  P+Y
Sbjct: 308 QKPEGESQCFGVFDVNMNPIY 325

[Close]

BLAST of Moc06g09390 vs. ExPASy TrEMBL
Match: A0A0A0LZ10 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.8e-114
Identity = 207/338 (61.24%), Postives = 256/338 (75.74%), Query Frame = 0


Query: 4   LGHVIIVVFFMA----ATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLD 63
           +  V+ +VF MA    A + VGA+D+LLGAYYGL G+NLPPPW VVQLC + NIRRVRLD
Sbjct: 1   MAKVVQIVFLMASILMAATVVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLD 60

Query: 64  EPNLDVFQAFRGAEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGD 123
            P++DV  AFRG  ID+SFG+PNNML ++  N   VEEWFNTYV+ F+ DF INYI+VGD
Sbjct: 61  VPDVDVLTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGD 120

Query: 124 KAIPGLEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDN 183
           KAIP   + ILP+M SLQDLLN++YLGQVKLTTLVGYN+AL V    PS+ TF+P   ++
Sbjct: 121 KAIPSHANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAAL-VSKDPPSSGTFDPTVRES 180

Query: 184 IKGILKFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDA 243
           ++GILKFL  EGS LMVSV+P +KY +N    +SL+YA+F     VV+D  + YYNLFDA
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHQKYEFNG--GISLNYAIFNETNPVVQDEGLEYYNLFDA 240

Query: 244 MVDAFYTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKK 303
           MVDAFY AIEK+ VG+V + VGETGWPTC    + T   A  YN  FK HI SG GTP+K
Sbjct: 241 MVDAFYAAIEKEMVGEVNIVVGETGWPTCG-DISGTQAIAKEYNNKFKSHISSGNGTPRK 300

Query: 304 QNIYIEGFIQSIFDENEKPEGDSRCYGMFHVDKIPVYP 338
            N+Y+EGFIQSIF+E++KPEGDS+CYGMF V+  P+YP
Sbjct: 301 PNVYLEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIYP 334

[Close]

BLAST of Moc06g09390 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 128/333 (38.44%), Postives = 205/333 (61.56%), Query Frame = 0


Query: 13  FMAATSAVGAHDVL--LGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQAFR 72
           F+ A +++G  +    +G  YGL G+NLP P  V+ L +  +I ++R+ +PN +V  A R
Sbjct: 27  FVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALR 86

Query: 73  G-AEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPG-LEDC 132
           G  +I ++ GV +  L  +  +  AV+ WF T +EP++ D  I +I VG++ IPG +   
Sbjct: 87  GHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQ 146

Query: 133 ILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFLF 192
           +LPVM+SL +L+ S  L  + ++T+V   +  +     PS   F   A + +  +LK L 
Sbjct: 147 VLPVMQSLTNLVKSRNL-PISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLS 206

Query: 193 VEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTAI 252
              SP++V +YP+  YA ++  ++ LDYA F ++  VV+DG + Y N+FDA+ DAF  A+
Sbjct: 207 QTNSPILVKIYPYFSYA-SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAM 266

Query: 253 EKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGFI 312
           EK+ V D+ + V ETGWP+  +G   TP+ A  YNRNF +HI SGKGTPK+ N  I+GF+
Sbjct: 267 EKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFL 326

Query: 313 QSIFDENEKPEGDSRCYGMFHV-DKIPVYPNVF 341
            + F+EN+KP G  + +G+++  D  P+Y N+F
Sbjct: 327 FATFNENQKPVGTEQNFGLYNPNDMKPIY-NLF 354

[Close]

BLAST of Moc06g09390 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 233.4 bits (594), Expect = 2.7e-61
Identity = 122/314 (38.85%), Postives = 195/314 (62.10%), Query Frame = 0


Query: 27  LGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQAFRG-AEIDLSFGVPNNML 86
           +G  YGL G+NLP P +V+ L +   I R+R+ +PN +V  A RG  +I+++ GV +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 87  INMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPG-LEDCILPVMKSLQDLLNSHY 146
             +  +  AV+ WF   +E ++ D  I +I VG++ IPG +   +LPVM+SL +L+ S  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 147 LGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFLFVEGSPLMVSVYPFRKY 206
           L  + ++T+V  ++  +     PS   F   A + +  +LK L    +P++V++YP+  +
Sbjct: 154 L-PISISTVVAMSN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY--F 213

Query: 207 AYNNDKA-VSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTAIEKQAVGDVAVAVGET 266
           AY +D A + LDYA F +K  VV+DG + Y N+FDA+ DAF  A+EK+ V ++ + V ET
Sbjct: 214 AYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSET 273

Query: 267 GWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGFIQSIFDENEKPEGDSR 326
           GWP+  +G   TP  A+ YNRNF +HI SGKGTPK+ N  + GF+ + F+EN+KP G  +
Sbjct: 274 GWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQ 333

Query: 327 CYGMFH-VDKIPVY 337
            +G+++  D  P+Y
Sbjct: 334 NFGLYNPSDMKPIY 342

[Close]

BLAST of Moc06g09390 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.7e-61
Identity = 127/333 (38.14%), Postives = 195/333 (58.56%), Query Frame = 0


Query: 9   IVVFFMAATSAVGAHDVL--LGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVF 68
           + V F+   S VG  D    +G  YGL G+NLPPP +V+ L +  ++  +R+ +   DV 
Sbjct: 13  LCVAFLLNYSNVGFVDAATNIGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVL 72

Query: 69  QAFRG-AEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPG- 128
            AFRG   I +   V N  L  +  +  AV  WF T +EP++ D  I +I VG++ IPG 
Sbjct: 73  NAFRGNRNIGVMVDVKNQDLEALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGE 132

Query: 129 LEDCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGIL 188
           +   +LPVMKSL +++ S  L  +  TT+   N   +     PS   F P A + +  +L
Sbjct: 133 IGSYVLPVMKSLTNIVKSRSLPILISTTVAMTN---LGQSYPPSAGDFMPQAREQLTPVL 192

Query: 189 KFLFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAF 248
           KFL    +P++V++YP+  YA +    + LDYA+F +   VV+DG + Y N+FD + DAF
Sbjct: 193 KFLSQTNTPILVNIYPYFAYAADPIN-IQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAF 252

Query: 249 YTAIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYI 308
             A+EK+ V D+ + V ETGWP+  +G   TP+ A++YN NF +H+ SGKGTPK+    I
Sbjct: 253 VWAMEKEGVKDLPMVVTETGWPSAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGI 312

Query: 309 EGFIQSIFDENEKPEGDSRCYGMFH-VDKIPVY 337
            GF+ + F+EN+KP G  + +G+++  D  P+Y
Sbjct: 313 SGFLFATFNENQKPAGTEQNFGLYNPTDMKPIY 341

[Close]

BLAST of Moc06g09390 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.1e-58
Identity = 122/314 (38.85%), Postives = 189/314 (60.19%), Query Frame = 0


Query: 9   IVVFFMAATSAVGAHDVLLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQA 68
           I++ F   TS   A    +G  YGL G+N P P +V+ L +   I ++R+ +P  +V  A
Sbjct: 16  IMLTFNYNTSGFVAAANSIGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNA 75

Query: 69  FRG-AEIDLSFGVPNNMLINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPG-LE 128
            RG   I+++ GV +  L  +  N  AV+ WF T +EP++ D  I +I VG++ IPG + 
Sbjct: 76  LRGHRNIEVTVGVRDQDLGALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGPIG 135

Query: 129 DCILPVMKSLQDLLNSHYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKF 188
             +LPVM+SL  L+ S  L  + ++T+V  ++  +     PS   F   A + +  +LK 
Sbjct: 136 PQVLPVMQSLTILVKSMNL-PISISTVVAMSN--LEQSYPPSAGEFTSQAREQLVPVLKL 195

Query: 189 LFVEGSPLMVSVYPFRKYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYT 248
           L    +P++V++YP+  YA ++   + LDYA F +K  VV+DG + Y N+FDA+ DAF  
Sbjct: 196 LSQTSTPILVNIYPYFPYA-SDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVW 255

Query: 249 AIEKQAVGDVAVAVGETGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEG 308
           A+EK+ V D+ + V ETGWP+  +G   TP+ A  YNRNF +HI SGKGTPK+ N  ++G
Sbjct: 256 AMEKEGVKDLPMVVSETGWPSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDG 315

Query: 309 FIQSIFDENEKPEG 321
           F+ + F+EN+KP G
Sbjct: 316 FLFATFNENQKPAG 325

[Close]

BLAST of Moc06g09390 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.8e-58
Identity = 117/306 (38.24%), Postives = 184/306 (60.13%), Query Frame = 0


Query: 26  LLGAYYGLAGNNLPPPWDVVQLCRKNNIRRVRLDEPNLDVFQAFRG-AEIDLSFGVPNNM 85
           ++G  YGL G+NLP P +V++  +  N+ ++R+ EPN DV  A RG  +I ++ G+ N  
Sbjct: 33  VVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNED 92

Query: 86  LINMKTNSTAVEEWFNTYVEPFIGDFTINYIVVGDKAIPGLE--DCILPVMKSLQDLLNS 145
           L  +  N  AV+ WF+T ++P+I D  I +I VG++AIPG +    +LPV++SL DL+ S
Sbjct: 93  LEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKS 152

Query: 146 HYLGQVKLTTLVGYNSALVVDGGLPSNATFNPVASDNIKGILKFLFVEGSPLMVSVYPFR 205
             L Q+ ++T V   S   +    PS     P A   +  +L+ L    +P+ V++YP+ 
Sbjct: 153 RNL-QISISTTVTTTSLARLKP--PSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPYY 212

Query: 206 KYAYNNDKAVSLDYAMFRSKVAVVRDGEVSYYNLFDAMVDAFYTAIEKQAVGDVAVAVGE 265
            +A ++ K V L+YA F +   VV+DG + Y NLFDA+ DAF  A+EK+ V  + + V E
Sbjct: 213 FHA-SDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSE 272

Query: 266 TGWPTCDHGYAATPEYAALYNRNFKRHICSGKGTPKKQNIYIEGFIQSIFDENEKPEGDS 325
           TGWP+  +G   TP     Y  NF +H+ SGKGTPK+ N  I+ +I   ++EN+KP G  
Sbjct: 273 TGWPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIY 332

Query: 326 RCYGMF 329
           + +G++
Sbjct: 333 QHFGLY 334

The following BLAST results are available for this feature:

BLAST of Moc06g09390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136187.1	1.2e-132	69.73	probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia]	[more]
XP_038880473.1	4.4e-122	63.61	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
KAG6590489.1	8.3e-121	66.07	putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma s...	[more]
XP_038880685.1	2.4e-120	65.29	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
XP_022968751.1	3.1e-120	64.41	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima]	[more]

BLAST of Moc06g09390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49353	1.5e-61	38.44	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49352	3.8e-60	38.85	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	3.3e-56	37.31	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
P07979	4.5e-53	35.47	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
P52407	3.2e-51	36.80	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O...	[more]

BLAST of Moc06g09390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4V7	6.0e-133	69.73	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 ...	[more]
A0A6J1HVR6	1.5e-120	64.41	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=...	[more]
A0A1S3BQX5	4.6e-117	62.76	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV...	[more]
A0A5A7UKS6	6.6e-116	63.55	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0LZ10	2.8e-114	61.24	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3...	[more]

BLAST of Moc06g09390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20340.1	1.1e-62	38.44	beta-1,3-glucanase 5	[more]
AT5G20330.1	2.7e-61	38.85	beta-1,3-glucanase 4	[more]
AT5G20390.1	2.7e-61	38.14	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	2.1e-58	38.85	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	2.8e-58	38.24	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 27..336 e-value: 8.1E-62 score: 209.6
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 26..341 e-value: 3.6E-87 score: 294.3
None	No IPR available	PANTHER	PTHR32227:SF305	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 20..337
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 20..337
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 28..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g09390.1	Moc06g09390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds