Moc06g08450 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g08450
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionInositol oxygenase
Locationchr6: 6096643 .. 6098313 (-)
RNA-Seq ExpressionMoc06g08450
SyntenyMoc06g08450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGATCCTCATCGACCACCCTCACCTAATTGGTACCTTCTAAATTCTTTTCTCGCATGGAACCATGAATTTTAATTCCAATATGGGGTTTCTAATTTTGCGTTTGTTTTGATTGCAGAAGTGGAAGAGAAGAAGGGGGCGATTTCAGAGATGAAGGAATTGGCGTTGGACGGAGGATTCGTGATACCCGATAATCAAACGGCGGCGGCGGCGGCGGCGGAAGGTTTCGTGGCGCCGGAAATTAATTCGTTCGGCCAAAGTTTCAGAGACTACGCGGCGGAGAGCGATAGGCAGAAGAGCGTAGAGGAATTCTACCGAATGAACCACATTCACCAGACGTACGATTTCGTGAAGAAAATGAGGGAACAATACGGCAAAATGGATCGCGTGGAAATGAGCATATGGGAATGCTGTGAGCTCCTAAACGACGTCGTTGATGACAGCGATCCTGATTTGGACGAACCCCAGATTCAGCATTTGCTGCAAACCGCCGAAGCCATTCGAAAAGATTACCCAGATCAAGATTGGCTGCATTTGACTGCTCTCATTCATGGTACAATTCATTTACACAAAACAGAGTGCCCTCTGTTTTCCTCTGTTTTTTCTTTTCTTAACGATTTTGCTCTGTTTTCAGATCTTGGAAAAGTGCTTCTTCTGCCAAGTTTTGGAGGGCTTCCCCAATGGGCTGTTGTGGGTAAGAATTAAGAACCAACAACAACAAATTTCTGGGATTCTTGAGTTTTTTTTTGGCCCGTTAATTGAATGGGGTGATTTGGTTTTGCAGGGGATACACACCCTCTTGGCTGCGCTTTTGACGAATCCATTGTTCACCACAAGGTCGAATTGAGAATTGGGATTGGTTTTTAAGTATGGGAGAATTTGAATCGCCAAATTGGGAATTTCAGCTTACAAAAAGTTTTGATTTTGGATTATGATTTCAGTATTTCAAGGAAAATTCTGACTCCGAGAATCCAGCTTATAATACCAAAAATGGCATTTACTCTGAAGGCTGTGGACTAGATAACGTTATGATATCGTGGGGCCACGATGACTATATGTATTTGGTATGAAATATAATCTTTTTTTGTACCCTTTTTTTTTTGGATAAAAGCTCTGTTTTTTAACATTAATCTCTGTGGTAATTTTTGCAGGTAGCTAAAGAAAATGGGTCGACTTTGCCTTCAGCTGGATTGTTCATAATCCGTTACCATTCTTTTTATCGTAAGTGAAAAAAAAAAGAGAATTTATAATTTTGAGTTTTGGGTATAGAATAGAATCTTAAACAGAAAAAAAAAAAAAATTGGCAGCTTTACATAAATCGGGAGCATACAAACACCTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTTCATATATTCAAGTAATTGATGATTATTTTGTTAATTAAAATTACTATTATTGTGCAAAATAATTGGATTAACTCACTGTTTGTTTATGAAATTTCAGCAAGTATGATCTCTACAGTAAGAGCAAAGAAAGAATTGACGTAGAAAAAGTTAAACCATACTATATGTCCCTTATTAACAAGGTAAATCCTGTGCCTTTTATTTATTAATTTACATGTTTTTCACGATATATTTTGGTAACTAAATGCTGTAAATTTTTGGGGTTTGCAGTACTTCCCGGAGAAACTCAGATGGTGA

mRNA sequence

ATGACGATCCTCATCGACCACCCTCACCTAATTGAAGTGGAAGAGAAGAAGGGGGCGATTTCAGAGATGAAGGAATTGGCGTTGGACGGAGGATTCGTGATACCCGATAATCAAACGGCGGCGGCGGCGGCGGCGGAAGGTTTCGTGGCGCCGGAAATTAATTCGTTCGGCCAAAGTTTCAGAGACTACGCGGCGGAGAGCGATAGGCAGAAGAGCGTAGAGGAATTCTACCGAATGAACCACATTCACCAGACGTACGATTTCGTGAAGAAAATGAGGGAACAATACGGCAAAATGGATCGCGTGGAAATGAGCATATGGGAATGCTGTGAGCTCCTAAACGACGTCGTTGATGACAGCGATCCTGATTTGGACGAACCCCAGATTCAGCATTTGCTGCAAACCGCCGAAGCCATTCGAAAAGATTACCCAGATCAAGATTGGCTGCATTTGACTGCTCTCATTCATGATCTTGGAAAAGTGCTTCTTCTGCCAAGTTTTGGAGGGCTTCCCCAATGGGCTGTTGTGGGGGATACACACCCTCTTGGCTGCGCTTTTGACGAATCCATTGTTCACCACAAGTATTTCAAGGAAAATTCTGACTCCGAGAATCCAGCTTATAATACCAAAAATGGCATTTACTCTGAAGGCTGTGGACTAGATAACGTTATGATATCGTGGGGCCACGATGACTATATGTATTTGGTAGCTAAAGAAAATGGGTCGACTTTGCCTTCAGCTGGATTGTTCATAATCCGTTACCATTCTTTTTATCCTTTACATAAATCGGGAGCATACAAACACCTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTTCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAAAGAATTGACGTAGAAAAAGTTAAACCATACTATATGTCCCTTATTAACAAGTACTTCCCGGAGAAACTCAGATGGTGA

Coding sequence (CDS)

ATGACGATCCTCATCGACCACCCTCACCTAATTGAAGTGGAAGAGAAGAAGGGGGCGATTTCAGAGATGAAGGAATTGGCGTTGGACGGAGGATTCGTGATACCCGATAATCAAACGGCGGCGGCGGCGGCGGCGGAAGGTTTCGTGGCGCCGGAAATTAATTCGTTCGGCCAAAGTTTCAGAGACTACGCGGCGGAGAGCGATAGGCAGAAGAGCGTAGAGGAATTCTACCGAATGAACCACATTCACCAGACGTACGATTTCGTGAAGAAAATGAGGGAACAATACGGCAAAATGGATCGCGTGGAAATGAGCATATGGGAATGCTGTGAGCTCCTAAACGACGTCGTTGATGACAGCGATCCTGATTTGGACGAACCCCAGATTCAGCATTTGCTGCAAACCGCCGAAGCCATTCGAAAAGATTACCCAGATCAAGATTGGCTGCATTTGACTGCTCTCATTCATGATCTTGGAAAAGTGCTTCTTCTGCCAAGTTTTGGAGGGCTTCCCCAATGGGCTGTTGTGGGGGATACACACCCTCTTGGCTGCGCTTTTGACGAATCCATTGTTCACCACAAGTATTTCAAGGAAAATTCTGACTCCGAGAATCCAGCTTATAATACCAAAAATGGCATTTACTCTGAAGGCTGTGGACTAGATAACGTTATGATATCGTGGGGCCACGATGACTATATGTATTTGGTAGCTAAAGAAAATGGGTCGACTTTGCCTTCAGCTGGATTGTTCATAATCCGTTACCATTCTTTTTATCCTTTACATAAATCGGGAGCATACAAACACCTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTTCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAAAGAATTGACGTAGAAAAAGTTAAACCATACTATATGTCCCTTATTAACAAGTACTTCCCGGAGAAACTCAGATGGTGA

Protein sequence

MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSFRDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVEKVKPYYMSLINKYFPEKLRW
Homology
BLAST of Moc06g08450 vs. NCBI nr
Match: XP_022144825.1 (inositol oxygenase 4-like [Momordica charantia])

HSP 1 Score: 667.9 bits (1722), Expect = 4.3e-188
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF
Sbjct: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYYMSLINKYFPEKLRW
Sbjct: 301 KVKPYYMSLINKYFPEKLRW 320

BLAST of Moc06g08450 vs. NCBI nr
Match: XP_023515618.1 (inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol oxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597.0 bits (1538), Expect = 9.3e-167
Identity = 286/320 (89.38%), Postives = 300/320 (93.75%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ P   EVEEKK  I+E+ EL LDGGFV+P N+TAAAAA EGFVA E+N+FG SF
Sbjct: 1   MTILIEQPR-FEVEEKKVGITELNELVLDGGFVVP-NETAAAAAEEGFVALEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE IDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPEKLRW
Sbjct: 301 KVKPYYVSLINKYFPEKLRW 318

BLAST of Moc06g08450 vs. NCBI nr
Match: XP_022960634.1 (inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 589.3 bits (1518), Expect = 1.9e-164
Identity = 284/320 (88.75%), Postives = 298/320 (93.12%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ P   EVEEKK  I+E+ EL LDGGFV+P N+T  AAA EGFVA E+N+FG SF
Sbjct: 1   MTILIEQPR-FEVEEKKVGITELNELVLDGGFVVP-NET--AAAEEGFVALEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE IDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPEKLRW
Sbjct: 301 KVKPYYVSLINKYFPEKLRW 316

BLAST of Moc06g08450 vs. NCBI nr
Match: XP_008450805.1 (PREDICTED: inositol oxygenase 2-like [Cucumis melo])

HSP 1 Score: 581.3 bits (1497), Expect = 5.3e-162
Identity = 274/320 (85.62%), Postives = 294/320 (91.88%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ PH    +EKK  ++E+KEL LDGGFV+P+ +T      EGF APE+N+FG SF
Sbjct: 1   MTILIEQPHFEVEDEKKVGMTELKELVLDGGFVVPNART-----TEGFAAPEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFYR NHIHQTY+FV KMRE+Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           P+GCAFDESIVHHKYFKEN D ENP Y+TKNGIYSEGCGL+NVMISWGHDDYMYLVAKEN
Sbjct: 181 PVGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLH++GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE IDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPEKLRW
Sbjct: 301 KVKPYYISLINKYFPEKLRW 315

BLAST of Moc06g08450 vs. NCBI nr
Match: XP_022987889.1 (inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase 2-like [Cucurbita maxima])

HSP 1 Score: 581.3 bits (1497), Expect = 5.3e-162
Identity = 279/320 (87.19%), Postives = 292/320 (91.25%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ P   EVEEKK  I E  EL LDGGFV+P+      AA EGFVA E+N+FG SF
Sbjct: 1   MTILIEQPR-FEVEEKKLGIIEPNELVLDGGFVVPN---GTEAAEEGFVALEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE +DVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPE LRW
Sbjct: 301 KVKPYYVSLINKYFPENLRW 316

BLAST of Moc06g08450 vs. ExPASy Swiss-Prot
Match: Q8H1S0 (Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 6.3e-142
Identity = 238/320 (74.38%), Postives = 268/320 (83.75%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTI ++ P   EV   + +   + EL LDGGF +P   T      E F+APE+N+FG+ F
Sbjct: 1   MTISVEKPIFEEVSAFEKSGDNIGELKLDGGFSMPKMDT---NDDEAFLAPEMNAFGRQF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY  ES+RQK VEEFYR+ HI+QT DFVKKMR +YGK+D++ MSIWECCELLN+VVD+S
Sbjct: 61  RDYDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDES 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQ+AEAIRKDYP++DWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT 
Sbjct: 121 DPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTF 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           P+GCAFDES VHHKYF EN D  N  YNTKNGIYSEGCGL+NVM+SWGHDDYMYLVAKEN
Sbjct: 181 PVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAG FIIRYHSFYPLH +G Y HLMNEED +NLKWLH+FNKYDLYSKSK  +DVE
Sbjct: 241 GSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYYMSLI KYFPE LRW
Sbjct: 301 KVKPYYMSLIKKYFPENLRW 317

BLAST of Moc06g08450 vs. ExPASy Swiss-Prot
Match: O82200 (Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2)

HSP 1 Score: 504.2 bits (1297), Expect = 1.1e-141
Identity = 236/323 (73.07%), Postives = 274/323 (84.83%), Query Frame = 0

Query: 1   MTILIDH--PHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQ 60
           MTIL++H  P    V+EKK       EL LDGGFV+P ++       + F AP++N  G 
Sbjct: 1   MTILVEHFVPD-SRVDEKKVIEERDNELVLDGGFVVPKSK-----ETDAFDAPDMNFLGH 60

Query: 61  SFRDYA-AESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVV 120
           SFRDY   ES+RQ+ VEEFYRM HIHQTYDFVKKMR++YGK++++EMSIWECCELLN+VV
Sbjct: 61  SFRDYENGESERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVV 120

Query: 121 DDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVG 180
           D+SDPDLDEPQIQHLLQTAEAIR+DYPD+DWLHLTALIHDLGKVLLLP FGGLPQWAVVG
Sbjct: 121 DESDPDLDEPQIQHLLQTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVG 180

Query: 181 DTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVA 240
           DT P+GC FD + +HHKYFK N D  NP YNTKNG+Y+EGCGLDNV++SWGHDDYMYLVA
Sbjct: 181 DTFPVGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVA 240

Query: 241 KENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERI 300
           K+NG+TLP AGLFIIRYHSFYPLHK+GAY HLMN+ED  +LKWLH+FNKYDLYSKSK  +
Sbjct: 241 KKNGTTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLV 300

Query: 301 DVEKVKPYYMSLINKYFPEKLRW 321
           DVE+VKPYY+SLINKYFP KL+W
Sbjct: 301 DVEQVKPYYISLINKYFPAKLKW 317

BLAST of Moc06g08450 vs. ExPASy Swiss-Prot
Match: Q5Z8T3 (Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0561000 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.2e-135
Identity = 229/324 (70.68%), Postives = 263/324 (81.17%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKK----GAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSF 60
           MTI I+ PHL  + ++K    G      EL LDGGFV+PD+                N+F
Sbjct: 1   MTITIEQPHLDAIADRKVAGGGGGDNAAELVLDGGFVVPDS----------------NAF 60

Query: 61  GQSFRDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDV 120
           G +FR+Y AES+R+++VEEFYR+NHI+QTYDFV++MRE+YG++D+ EM IWEC ELLN+ 
Sbjct: 61  GNAFRNYEAESERKETVEEFYRVNHINQTYDFVRRMREEYGRVDKTEMGIWECIELLNEF 120

Query: 121 VDDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVV 180
           +DDSDPDLD PQI+HLLQTAEAIRKD+PD+DWLHLT LIHDLGKVLL PSFG LPQW+VV
Sbjct: 121 IDDSDPDLDMPQIEHLLQTAEAIRKDFPDEDWLHLTGLIHDLGKVLLHPSFGELPQWSVV 180

Query: 181 GDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLV 240
           GDT P+GCAFDE  VH KYFKEN D  NP  NTK G YSEGCGLDNV++SWGHDDYMYLV
Sbjct: 181 GDTFPVGCAFDECNVHFKYFKENPDYLNPKLNTKFGAYSEGCGLDNVLMSWGHDDYMYLV 240

Query: 241 AKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKER 300
           AKEN +TLPSAGLFIIRYHSFYPLHK GAY HLMN+ED +NLKWL +FNKYDLYSKS ER
Sbjct: 241 AKENKTTLPSAGLFIIRYHSFYPLHKHGAYMHLMNDEDKENLKWLRVFNKYDLYSKSNER 300

Query: 301 IDVEKVKPYYMSLINKYFPEKLRW 321
           IDVEKVKPYYMSLI KYFP KLRW
Sbjct: 301 IDVEKVKPYYMSLIEKYFPAKLRW 308

BLAST of Moc06g08450 vs. ExPASy Swiss-Prot
Match: Q8L799 (Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 7.0e-133
Identity = 228/320 (71.25%), Postives = 264/320 (82.50%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILID        ++  A  E+ E     G    + +T     A GF AP  NSFG++F
Sbjct: 1   MTILIDRH-----SDQNDAGDEIVEKNQGNG---KEEETELVLDA-GFEAPHTNSFGRTF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+R++ VEEFYR+NHI QT DFV+KMRE+Y K++R EMSIWECCELLN+ +D+S
Sbjct: 61  RDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEFIDES 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQI+HLLQTAEAIRKDYPD+DWLHLT LIHDLGKVLL  SFG LPQWAVVGDT 
Sbjct: 121 DPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWAVVGDTF 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           P+GCAFDESIVHHKYFKEN D +NP+YN+K GIY+EGCGLDNV++SWGHDDYMYLVAKEN
Sbjct: 181 PVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
            +TLPSAGLFIIRYHSFY LHKS AYKHLMN ED +N+KWL +FNKYDLYSKSK R++VE
Sbjct: 241 QTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKVRVNVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           +VKPYY+SL NKYFP KL+W
Sbjct: 301 EVKPYYLSLTNKYFPSKLKW 311

BLAST of Moc06g08450 vs. ExPASy Swiss-Prot
Match: Q9FJU4 (Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 3.5e-132
Identity = 226/321 (70.40%), Postives = 261/321 (81.31%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           M I +++P  +    +     +  EL LD    IP   +  ++  E F+APE+N+FG+ F
Sbjct: 1   MNISVENPVFV---HEDSTTQKTGELRLDSD--IP--MSKISSDDEVFLAPEMNAFGRQF 60

Query: 61  RDYA-AESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDD 120
           RDY    S+RQKSVE FY   H +QT DFV+KMR +YGK+D++ M+IWECCEL  +VVD+
Sbjct: 61  RDYTDTNSERQKSVEHFYATQHTNQTLDFVQKMRSEYGKLDKMVMNIWECCELSKEVVDE 120

Query: 121 SDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDT 180
           SDPDLDEPQIQHLLQ+AEAIRKDYP++DWLHLTALIHDLGKVL LP FGGLPQWAVVGDT
Sbjct: 121 SDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVLTLPQFGGLPQWAVVGDT 180

Query: 181 HPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKE 240
            P+GCAFDES VHHKYF EN D  NP YNTK GIYSEGCGL+NV++SWGHDDYMYLVAKE
Sbjct: 181 FPVGCAFDESNVHHKYFMENPDFNNPKYNTKAGIYSEGCGLENVLMSWGHDDYMYLVAKE 240

Query: 241 NGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDV 300
           NGSTLPS GLFIIRYHSFYPLHK+GAY HLMNEED +NLKWLH+FNKYDLYSKSK  ++V
Sbjct: 241 NGSTLPSPGLFIIRYHSFYPLHKAGAYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVNV 300

Query: 301 EKVKPYYMSLINKYFPEKLRW 321
           EKVKPYYMSLI KYFPE LRW
Sbjct: 301 EKVKPYYMSLIKKYFPENLRW 314

BLAST of Moc06g08450 vs. ExPASy TrEMBL
Match: A0A6J1CSQ8 (Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.1e-188
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF
Sbjct: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYYMSLINKYFPEKLRW
Sbjct: 301 KVKPYYMSLINKYFPEKLRW 320

BLAST of Moc06g08450 vs. ExPASy TrEMBL
Match: A0A6J1HBL6 (Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 9.4e-165
Identity = 284/320 (88.75%), Postives = 298/320 (93.12%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ P   EVEEKK  I+E+ EL LDGGFV+P N+T  AAA EGFVA E+N+FG SF
Sbjct: 1   MTILIEQPR-FEVEEKKVGITELNELVLDGGFVVP-NET--AAAEEGFVALEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE IDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPEKLRW
Sbjct: 301 KVKPYYVSLINKYFPEKLRW 316

BLAST of Moc06g08450 vs. ExPASy TrEMBL
Match: A0A6J1JI24 (Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.6e-162
Identity = 279/320 (87.19%), Postives = 292/320 (91.25%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ P   EVEEKK  I E  EL LDGGFV+P+      AA EGFVA E+N+FG SF
Sbjct: 1   MTILIEQPR-FEVEEKKLGIIEPNELVLDGGFVVPN---GTEAAEEGFVALEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE +DVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPE LRW
Sbjct: 301 KVKPYYVSLINKYFPENLRW 316

BLAST of Moc06g08450 vs. ExPASy TrEMBL
Match: A0A1S3BQR6 (Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.6e-162
Identity = 274/320 (85.62%), Postives = 294/320 (91.88%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ PH    +EKK  ++E+KEL LDGGFV+P+ +T      EGF APE+N+FG SF
Sbjct: 1   MTILIEQPHFEVEDEKKVGMTELKELVLDGGFVVPNART-----TEGFAAPEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFYR NHIHQTY+FV KMRE+Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           P+GCAFDESIVHHKYFKEN D ENP Y+TKNGIYSEGCGL+NVMISWGHDDYMYLVAKEN
Sbjct: 181 PVGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLH++GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE IDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPEKLRW
Sbjct: 301 KVKPYYISLINKYFPEKLRW 315

BLAST of Moc06g08450 vs. ExPASy TrEMBL
Match: A0A0A0LZA8 (Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.3e-162
Identity = 274/320 (85.62%), Postives = 293/320 (91.56%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILI+ PH    +EKK  I+E+KEL LDGGFV+P+ +T      EGF APE+N+FG SF
Sbjct: 1   MTILIEQPHFEGEDEKKVGITELKELVLDGGFVVPNAKT------EGFAAPEMNAFGHSF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFYR NHIHQTY+FV+KMRE+Y KMDR EMSIWECCELLNDVVDDS
Sbjct: 61  RDYEAESERQKGVEEFYRQNHIHQTYEFVEKMREEYKKMDREEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           P+GCAFDESIVHHKYFKEN D ENP Y+TKNGIYSEGCGLDNV ISWGHDDYMYLVAKEN
Sbjct: 181 PVGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLDNVKISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAGLFIIRYHSFYPLH++GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE IDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYY+SLINKYFPEKLRW
Sbjct: 301 KVKPYYISLINKYFPEKLRW 314

BLAST of Moc06g08450 vs. TAIR 10
Match: AT4G26260.1 (myo-inositol oxygenase 4 )

HSP 1 Score: 505.0 bits (1299), Expect = 4.5e-143
Identity = 238/320 (74.38%), Postives = 268/320 (83.75%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTI ++ P   EV   + +   + EL LDGGF +P   T      E F+APE+N+FG+ F
Sbjct: 1   MTISVEKPIFEEVSAFEKSGDNIGELKLDGGFSMPKMDT---NDDEAFLAPEMNAFGRQF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY  ES+RQK VEEFYR+ HI+QT DFVKKMR +YGK+D++ MSIWECCELLN+VVD+S
Sbjct: 61  RDYDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDES 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQ+AEAIRKDYP++DWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT 
Sbjct: 121 DPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTF 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           P+GCAFDES VHHKYF EN D  N  YNTKNGIYSEGCGL+NVM+SWGHDDYMYLVAKEN
Sbjct: 181 PVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
           GSTLPSAG FIIRYHSFYPLH +G Y HLMNEED +NLKWLH+FNKYDLYSKSK  +DVE
Sbjct: 241 GSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           KVKPYYMSLI KYFPE LRW
Sbjct: 301 KVKPYYMSLIKKYFPENLRW 317

BLAST of Moc06g08450 vs. TAIR 10
Match: AT2G19800.1 (myo-inositol oxygenase 2 )

HSP 1 Score: 504.2 bits (1297), Expect = 7.6e-143
Identity = 236/323 (73.07%), Postives = 274/323 (84.83%), Query Frame = 0

Query: 1   MTILIDH--PHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQ 60
           MTIL++H  P    V+EKK       EL LDGGFV+P ++       + F AP++N  G 
Sbjct: 1   MTILVEHFVPD-SRVDEKKVIEERDNELVLDGGFVVPKSK-----ETDAFDAPDMNFLGH 60

Query: 61  SFRDYA-AESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVV 120
           SFRDY   ES+RQ+ VEEFYRM HIHQTYDFVKKMR++YGK++++EMSIWECCELLN+VV
Sbjct: 61  SFRDYENGESERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVV 120

Query: 121 DDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVG 180
           D+SDPDLDEPQIQHLLQTAEAIR+DYPD+DWLHLTALIHDLGKVLLLP FGGLPQWAVVG
Sbjct: 121 DESDPDLDEPQIQHLLQTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVG 180

Query: 181 DTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVA 240
           DT P+GC FD + +HHKYFK N D  NP YNTKNG+Y+EGCGLDNV++SWGHDDYMYLVA
Sbjct: 181 DTFPVGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVA 240

Query: 241 KENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERI 300
           K+NG+TLP AGLFIIRYHSFYPLHK+GAY HLMN+ED  +LKWLH+FNKYDLYSKSK  +
Sbjct: 241 KKNGTTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLV 300

Query: 301 DVEKVKPYYMSLINKYFPEKLRW 321
           DVE+VKPYY+SLINKYFP KL+W
Sbjct: 301 DVEQVKPYYISLINKYFPAKLKW 317

BLAST of Moc06g08450 vs. TAIR 10
Match: AT4G26260.2 (myo-inositol oxygenase 4 )

HSP 1 Score: 501.5 bits (1290), Expect = 5.0e-142
Identity = 239/323 (73.99%), Postives = 268/323 (82.97%), Query Frame = 0

Query: 1   MTILIDHP---HLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFG 60
           MTI ++ P     +   EK G    + EL LDGGF +P   T      E F+APE+N+FG
Sbjct: 1   MTISVEKPIFEEEVSAFEKSG--DNIGELKLDGGFSMPKMDT---NDDEAFLAPEMNAFG 60

Query: 61  QSFRDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVV 120
           + FRDY  ES+RQK VEEFYR+ HI+QT DFVKKMR +YGK+D++ MSIWECCELLN+VV
Sbjct: 61  RQFRDYDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVV 120

Query: 121 DDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVG 180
           D+SDPDLDEPQIQHLLQ+AEAIRKDYP++DWLHLTALIHDLGKV+ LP FGGLPQWAVVG
Sbjct: 121 DESDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVG 180

Query: 181 DTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVA 240
           DT P+GCAFDES VHHKYF EN D  N  YNTKNGIYSEGCGL+NVM+SWGHDDYMYLVA
Sbjct: 181 DTFPVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVA 240

Query: 241 KENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERI 300
           KENGSTLPSAG FIIRYHSFYPLH +G Y HLMNEED +NLKWLH+FNKYDLYSKSK  +
Sbjct: 241 KENGSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHV 300

Query: 301 DVEKVKPYYMSLINKYFPEKLRW 321
           DVEKVKPYYMSLI KYFPE LRW
Sbjct: 301 DVEKVKPYYMSLIKKYFPENLRW 318

BLAST of Moc06g08450 vs. TAIR 10
Match: AT1G14520.2 (myo-inositol oxygenase 1 )

HSP 1 Score: 475.7 bits (1223), Expect = 2.9e-134
Identity = 230/325 (70.77%), Postives = 263/325 (80.92%), Query Frame = 0

Query: 1   MTILIDH---PHLIEVEEKK--GAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINS 60
           MTILID     +  E+ EK       E  EL LD                 GF AP  NS
Sbjct: 1   MTILIDRHSDQNGDEIVEKNQGNGKEEETELVLDA----------------GFEAPHTNS 60

Query: 61  FGQSFRDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLND 120
           FG++FRDY AES+R++ VEEFYR+NHI QT DFV+KMRE+Y K++R EMSIWECCELLN+
Sbjct: 61  FGRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNE 120

Query: 121 VVDDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAV 180
            +D+SDPDLDEPQI+HLLQTAEAIRKDYPD+DWLHLT LIHDLGKVLL  SFG LPQWAV
Sbjct: 121 FIDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWAV 180

Query: 181 VGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYL 240
           VGDT P+GCAFDESIVHHKYFKEN D +NP+YN+K GIY+EGCGLDNV++SWGHDDYMYL
Sbjct: 181 VGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYL 240

Query: 241 VAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE 300
           VAKEN +TLPSAGLFIIRYHSFY LHKS AYKHLMN ED +N+KWL +FNKYDLYSKSK 
Sbjct: 241 VAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKV 300

Query: 301 RIDVEKVKPYYMSLINKYFPEKLRW 321
           R++VE+VKPYY+SL NKYFP KL+W
Sbjct: 301 RVNVEEVKPYYLSLTNKYFPSKLKW 309

BLAST of Moc06g08450 vs. TAIR 10
Match: AT1G14520.1 (myo-inositol oxygenase 1 )

HSP 1 Score: 474.9 bits (1221), Expect = 5.0e-134
Identity = 228/320 (71.25%), Postives = 264/320 (82.50%), Query Frame = 0

Query: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60
           MTILID        ++  A  E+ E     G    + +T     A GF AP  NSFG++F
Sbjct: 1   MTILIDRH-----SDQNDAGDEIVEKNQGNG---KEEETELVLDA-GFEAPHTNSFGRTF 60

Query: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+R++ VEEFYR+NHI QT DFV+KMRE+Y K++R EMSIWECCELLN+ +D+S
Sbjct: 61  RDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEFIDES 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQI+HLLQTAEAIRKDYPD+DWLHLT LIHDLGKVLL  SFG LPQWAVVGDT 
Sbjct: 121 DPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWAVVGDTF 180

Query: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240
           P+GCAFDESIVHHKYFKEN D +NP+YN+K GIY+EGCGLDNV++SWGHDDYMYLVAKEN
Sbjct: 181 PVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300
            +TLPSAGLFIIRYHSFY LHKS AYKHLMN ED +N+KWL +FNKYDLYSKSK R++VE
Sbjct: 241 QTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKVRVNVE 300

Query: 301 KVKPYYMSLINKYFPEKLRW 321
           +VKPYY+SL NKYFP KL+W
Sbjct: 301 EVKPYYLSLTNKYFPSKLKW 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144825.14.3e-188100.00inositol oxygenase 4-like [Momordica charantia][more]
XP_023515618.19.3e-16789.38inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol ox... [more]
XP_022960634.11.9e-16488.75inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase ... [more]
XP_008450805.15.3e-16285.63PREDICTED: inositol oxygenase 2-like [Cucumis melo][more]
XP_022987889.15.3e-16287.19inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase ... [more]
Match NameE-valueIdentityDescription
Q8H1S06.3e-14274.38Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1[more]
O822001.1e-14173.07Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2[more]
Q5Z8T31.2e-13570.68Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g056... [more]
Q8L7997.0e-13371.25Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1[more]
Q9FJU43.5e-13270.40Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CSQ82.1e-188100.00Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1[more]
A0A6J1HBL69.4e-16588.75Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1[more]
A0A6J1JI242.6e-16287.19Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1[more]
A0A1S3BQR62.6e-16285.63Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1[more]
A0A0A0LZA83.3e-16285.63Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G26260.14.5e-14374.38myo-inositol oxygenase 4 [more]
AT2G19800.17.6e-14373.07myo-inositol oxygenase 2 [more]
AT4G26260.25.0e-14273.99myo-inositol oxygenase 4 [more]
AT1G14520.22.9e-13470.77myo-inositol oxygenase 1 [more]
AT1G14520.15.0e-13471.25myo-inositol oxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007828Inositol oxygenasePFAMPF05153MIOXcoord: 71..320
e-value: 2.3E-125
score: 417.1
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 1..320
NoneNo IPR availablePANTHERPTHR12588:SF14INOSITOL OXYGENASE 5coord: 1..320
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 63..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g08450.1Moc06g08450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019310 inositol catabolic process
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050113 inositol oxygenase activity
molecular_function GO:0005506 iron ion binding