Moc06g06630 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g06630
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptiondual specificity tyrosine-phosphorylation-regulated kinase 3-like
Locationchr6: 4857856 .. 4858470 (+)
RNA-Seq ExpressionMoc06g06630
SyntenyMoc06g06630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAATCCAGTGATGCCTCGGAGGTGAACGAGCCAAATCGTTACTCTTTCAACTTGCACGATTTCTTGAAAACGAAGCGGCGGATTGTCAAGTGTGCGAAGAGGACGAAACCGGGGAATATGATCGTCAAAAAAAGATGTGGCTCTGGCTTTGGCTCTGGCTCTGGCTCTGGCCTCGTTTCCCTGGCCAAGAACAGAGCACTCTGCAACTTGTATTCAAATCTTCCCATACACATTGTTGTCAAATCTTCTTCCATGGAATTTTCCACCTCAAAAGGTCGCATTACTCTCAGATTTGGATCCAACGGGCTGCCAAGGCTCATGAAAGACATTTTACGCGGCCTCTCTAACATTCACTGCGACATAAAACCAAGAAATATGTTATTGTTACCCGGAGGTCGCGGAATCGAGACCGATTCTTCTTCGGCGGATTTTGGATTGGGTAAAAAGTTTGAAATTAGCGGTCGGCCTGCGGATATTTGGGCATTTGGATGCGTGGTGCTGGAGATGTTGACTGGGAAACGCCCAGAGTGGAGATTTGGTAATAGTTATGAGCATCTTTTGGATTATTACAGCACAACAACTGATCGACTTGCCATTAATTCCAATTGA

mRNA sequence

ATGACGAAATCCAGTGATGCCTCGGAGGTGAACGAGCCAAATCGTTACTCTTTCAACTTGCACGATTTCTTGAAAACGAAGCGGCGGATTGTCAAGTGTGCGAAGAGGACGAAACCGGGGAATATGATCGTCAAAAAAAGATGTGGCTCTGGCTTTGGCTCTGGCTCTGGCTCTGGCCTCGTTTCCCTGGCCAAGAACAGAGCACTCTGCAACTTGTATTCAAATCTTCCCATACACATTGTTGTCAAATCTTCTTCCATGGAATTTTCCACCTCAAAAGGTCGCATTACTCTCAGATTTGGATCCAACGGGCTGCCAAGGCTCATGAAAGACATTTTACGCGGCCTCTCTAACATTCACTGCGACATAAAACCAAGAAATATGTTATTGTTACCCGGAGGTCGCGGAATCGAGACCGATTCTTCTTCGGCGGATTTTGGATTGGGTAAAAAGTTTGAAATTAGCGGTCGGCCTGCGGATATTTGGGCATTTGGATGCGTGGTGCTGGAGATGTTGACTGGGAAACGCCCAGAGTGGAGATTTGGTAATAGTTATGAGCATCTTTTGGATTATTACAGCACAACAACTGATCGACTTGCCATTAATTCCAATTGA

Coding sequence (CDS)

ATGACGAAATCCAGTGATGCCTCGGAGGTGAACGAGCCAAATCGTTACTCTTTCAACTTGCACGATTTCTTGAAAACGAAGCGGCGGATTGTCAAGTGTGCGAAGAGGACGAAACCGGGGAATATGATCGTCAAAAAAAGATGTGGCTCTGGCTTTGGCTCTGGCTCTGGCTCTGGCCTCGTTTCCCTGGCCAAGAACAGAGCACTCTGCAACTTGTATTCAAATCTTCCCATACACATTGTTGTCAAATCTTCTTCCATGGAATTTTCCACCTCAAAAGGTCGCATTACTCTCAGATTTGGATCCAACGGGCTGCCAAGGCTCATGAAAGACATTTTACGCGGCCTCTCTAACATTCACTGCGACATAAAACCAAGAAATATGTTATTGTTACCCGGAGGTCGCGGAATCGAGACCGATTCTTCTTCGGCGGATTTTGGATTGGGTAAAAAGTTTGAAATTAGCGGTCGGCCTGCGGATATTTGGGCATTTGGATGCGTGGTGCTGGAGATGTTGACTGGGAAACGCCCAGAGTGGAGATTTGGTAATAGTTATGAGCATCTTTTGGATTATTACAGCACAACAACTGATCGACTTGCCATTAATTCCAATTGA

Protein sequence

MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNMIVKKRCGSGFGSGSGSGLVSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNIHCDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEISGRPADIWAFGCVVLEMLTGKRPEWRFGNSYEHLLDYYSTTTDRLAINSN
Homology
BLAST of Moc06g06630 vs. NCBI nr
Match: XP_022144818.1 (dual specificity tyrosine-phosphorylation-regulated kinase 3-like [Momordica charantia] >XP_022144819.1 dual specificity tyrosine-phosphorylation-regulated kinase 3-like [Momordica charantia])

HSP 1 Score: 416.0 bits (1068), Expect = 1.9e-112
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNMIVKKRCGSGFGSGSGSGL 60
           MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNMIVKKRCGSGFGSGSGSGL
Sbjct: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNMIVKKRCGSGFGSGSGSGL 60

Query: 61  VSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNIH 120
           VSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNIH
Sbjct: 61  VSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNIH 120

Query: 121 CDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEISGRPADIWAFGCVVLEMLTGKRPEWR 180
           CDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEISGRPADIWAFGCVVLEMLTGKRPEWR
Sbjct: 121 CDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEISGRPADIWAFGCVVLEMLTGKRPEWR 180

Query: 181 FGNSYEHLLDYYSTTTDRLAINSN 205
           FGNSYEHLLDYYSTTTDRLAINSN
Sbjct: 181 FGNSYEHLLDYYSTTTDRLAINSN 204

BLAST of Moc06g06630 vs. NCBI nr
Match: KAG6589837.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 114.0 bits (284), Expect = 1.5e-21
Identity = 104/294 (35.37%), Postives = 130/294 (44.22%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKP---------------GNMIVK 60
           MTKS   S+VN+      NLH+FLKTKR IVKCAK+++                GN  ++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  KRCGSGFGSG------SGSGLVSLAKNRALCNLYSNLPIHIVVKSSSMEFSTS------- 120
           + C   F  G      SGSGLV LAKN++  N  S      +VKS  ME S+S       
Sbjct: 61  E-CHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEV 120

Query: 121 --------------KGRIT-------------------------LRFGSNGLP-----RL 180
                            IT                         ++FG NGLP     + 
Sbjct: 121 LCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKY 180

Query: 181 MKDILRGLSNIHC-------DIKPRNMLLLPGGR-GIETDSSSADFGLGKKF-------- 191
            +DI+RGL  IHC       DIKPRN+LL P  R G    +  A FGL +K         
Sbjct: 181 TRDIVRGLCYIHCNAGYIHGDIKPRNILLSPSDRKGGCLVAKLASFGLARKLTPEVLCDE 240

BLAST of Moc06g06630 vs. NCBI nr
Match: XP_022960664.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 111.7 bits (278), Expect = 7.6e-21
Identity = 99/290 (34.14%), Postives = 132/290 (45.52%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKP---------------GNMIVK 60
           MTKS   S+VN+      NLH+FLKTKR IVKCAK+++                GN  ++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  KRCGSGFGSG------SGSGLVSLAKNRALCNLYSNLPIH--IVVKSSSMEF-------- 120
           + C   F  G      SGSGLV LAKN++  N  S   +   ++  SSS+++        
Sbjct: 61  E-CHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNSEFLVKSCLMESSSSLQYEKEVLCNL 120

Query: 121 ----------------STSKGRI----------------TLRFGSNGLP-----RLMKDI 180
                           S S  +I                 ++FG NGLP     +  +DI
Sbjct: 121 GPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDI 180

Query: 181 LRGLSNIHC-------DIKPRNMLLLPGG-RGIETDSSSADFGLGKKF--------EISG 191
           +RGL  +HC       DIKPRN+LL P   +G    +  A FGL +K         EISG
Sbjct: 181 VRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEEISG 240

BLAST of Moc06g06630 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 110.9 bits (276), Expect = 1.3e-20
Identity = 103/294 (35.03%), Postives = 131/294 (44.56%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKP---------------GNMIVK 60
           MTKS   S+VN+      NLH+FLKTKR IVKCAK+++                GN  ++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  KRCGSGFGSG------SGSGLVSLAKNRALCNLYSNLPIHIVVKSSSMEFSTS------- 120
           +  GS F  G      SGSGLV LAKN++  N  S      +VKS  ME S+S       
Sbjct: 61  EYHGS-FWIGDRLIRKSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEV 120

Query: 121 --------------KGRIT-------------------------LRFGSNGLP-----RL 180
                            IT                         ++FG NGLP     + 
Sbjct: 121 LCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKY 180

Query: 181 MKDILRGLSNIHC-------DIKPRNMLLLPGG-RGIETDSSSADFGLGKKF-------- 191
            +DI+RGL  +HC       DIKPRN+LL P   +G    +  A FGL +K         
Sbjct: 181 TRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDE 240

BLAST of Moc06g06630 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 108.6 bits (270), Expect = 6.4e-20
Identity = 104/294 (35.37%), Postives = 131/294 (44.56%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKP---------------GNMIVK 60
           MTKS   S+VN+      NLH+FLKTKR IVKCAK+++                GN  ++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60

Query: 61  KRCGSGFGSG------SGSGLVSLAKNRALCNLYSNLPIHIVVKSSSMEFSTS------- 120
           +  GS F  G      SGSGLV LAKN++  N  SN     +VKS  ME S+S       
Sbjct: 61  EYHGS-FWIGDRLIRKSGSGLVFLAKNKSSTN--SN--PEFLVKSCLMESSSSLQYEKEV 120

Query: 121 --------------KGRIT-------------------------LRFGSNGLP-----RL 180
                            IT                         ++FG NGLP     + 
Sbjct: 121 LRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQY 180

Query: 181 MKDILRGLSNIHC-------DIKPRNMLLLPGG-RGIETDSSSADFGLGKKF-------- 191
            +DI+ GL  +HC       DIKPRN+LL P   +G    +  A FGL +K         
Sbjct: 181 TRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDE 240

BLAST of Moc06g06630 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-05
Identity = 33/105 (31.43%), Postives = 48/105 (45.71%), Query Frame = 0

Query: 110 KDILRGLSNI------HCDIKPRNMLLLPGGRGIETDSSSADFGLGKKF----------- 169
           +DIL+GL  I      HCD+K  N+++   G     ++  ADFG  K+            
Sbjct: 108 RDILKGLEYIHSKGIVHCDVKGSNVVISEKG-----EAKIADFGCAKRVDPVFESPVMGT 167

Query: 170 -----------EISGRPADIWAFGCVVLEMLTGKRPEWRFGNSYE 187
                      E  G+ +DIWA GC ++EM+TG  P W   +S E
Sbjct: 168 PAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGS-PPWTKADSRE 206

BLAST of Moc06g06630 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.1e-05
Identity = 40/115 (34.78%), Postives = 47/115 (40.87%), Query Frame = 0

Query: 99  RFGSNGLP-----RLMKDILRGLSNI------HCDIKPRNMLLLPGGRGIETDSSSADFG 158
           + G  GLP     R    +LRGL +I      HCDIK  N+LL   G         ADFG
Sbjct: 97  KLGGEGLPESTVRRHTGSVLRGLRHIHAKGFAHCDIKLANILLFNDG-----SVKIADFG 156

Query: 159 LG-----------KKFEISGRP----------------ADIWAFGCVVLEMLTGK 176
           L            K  EI G P                AD+WA GC V+EM +GK
Sbjct: 157 LAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGK 206

BLAST of Moc06g06630 vs. ExPASy Swiss-Prot
Match: O43781 (Dual specificity tyrosine-phosphorylation-regulated kinase 3 OS=Homo sapiens OX=9606 GN=DYRK3 PE=1 SV=3)

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-05
Identity = 38/137 (27.74%), Postives = 58/137 (42.34%), Query Frame = 0

Query: 60  LVSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNI 119
           L S      +C  +  L I +       +F     ++  +F  + L  L  D L     I
Sbjct: 275 LESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSL--DALHKNKII 334

Query: 120 HCDIKPRNMLLLPGGRG----IETDSSSADFG-----------------LGKKFEISGRP 176
           HCD+KP N+LL   GR     I+  SS  ++                  LG ++     P
Sbjct: 335 HCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGSRY---STP 394

BLAST of Moc06g06630 vs. ExPASy Swiss-Prot
Match: Q4R6S5 (Dual specificity tyrosine-phosphorylation-regulated kinase 3 OS=Macaca fascicularis OX=9541 GN=DYRK3 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-05
Identity = 38/137 (27.74%), Postives = 58/137 (42.34%), Query Frame = 0

Query: 60  LVSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNI 119
           L S      +C  +  L I +       +F     ++  +F  + L  L  D L     I
Sbjct: 255 LESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSL--DALHKNKII 314

Query: 120 HCDIKPRNMLLLPGGRG----IETDSSSADFG-----------------LGKKFEISGRP 176
           HCD+KP N+LL   GR     I+  SS  ++                  LG ++     P
Sbjct: 315 HCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGSRY---STP 374

BLAST of Moc06g06630 vs. ExPASy Swiss-Prot
Match: Q922Y0 (Dual specificity tyrosine-phosphorylation-regulated kinase 3 OS=Mus musculus OX=10090 GN=Dyrk3 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 6.0e-05
Identity = 39/134 (29.10%), Postives = 56/134 (41.79%), Query Frame = 0

Query: 60  LVSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNI 119
           L S      +C  +  L I +       +F     ++  +F  + L  L  D L     I
Sbjct: 274 LESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSL--DALHKNKII 333

Query: 120 HCDIKPRNMLLLPGGRGIE--TDSSSADFGLGKKFE-ISGR---------------PADI 176
           HCD+KP N+LL   GR      D  S+ F   K +  I  R               P DI
Sbjct: 334 HCDLKPENILLKHHGRSATKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGCRYSTPIDI 393

BLAST of Moc06g06630 vs. ExPASy TrEMBL
Match: A0A6J1CUA9 (dual specificity tyrosine-phosphorylation-regulated kinase 3-like OS=Momordica charantia OX=3673 GN=LOC111014411 PE=4 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 9.1e-113
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNMIVKKRCGSGFGSGSGSGL 60
           MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNMIVKKRCGSGFGSGSGSGL
Sbjct: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNMIVKKRCGSGFGSGSGSGL 60

Query: 61  VSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNIH 120
           VSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNIH
Sbjct: 61  VSLAKNRALCNLYSNLPIHIVVKSSSMEFSTSKGRITLRFGSNGLPRLMKDILRGLSNIH 120

Query: 121 CDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEISGRPADIWAFGCVVLEMLTGKRPEWR 180
           CDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEISGRPADIWAFGCVVLEMLTGKRPEWR
Sbjct: 121 CDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEISGRPADIWAFGCVVLEMLTGKRPEWR 180

Query: 181 FGNSYEHLLDYYSTTTDRLAINSN 205
           FGNSYEHLLDYYSTTTDRLAINSN
Sbjct: 181 FGNSYEHLLDYYSTTTDRLAINSN 204

BLAST of Moc06g06630 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 3.7e-21
Identity = 99/290 (34.14%), Postives = 132/290 (45.52%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKP---------------GNMIVK 60
           MTKS   S+VN+      NLH+FLKTKR IVKCAK+++                GN  ++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  KRCGSGFGSG------SGSGLVSLAKNRALCNLYSNLPIH--IVVKSSSMEF-------- 120
           + C   F  G      SGSGLV LAKN++  N  S   +   ++  SSS+++        
Sbjct: 61  E-CHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNSEFLVKSCLMESSSSLQYEKEVLCNL 120

Query: 121 ----------------STSKGRI----------------TLRFGSNGLP-----RLMKDI 180
                           S S  +I                 ++FG NGLP     +  +DI
Sbjct: 121 GPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDI 180

Query: 181 LRGLSNIHC-------DIKPRNMLLLPGG-RGIETDSSSADFGLGKKF--------EISG 191
           +RGL  +HC       DIKPRN+LL P   +G    +  A FGL +K         EISG
Sbjct: 181 VRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEEISG 240

BLAST of Moc06g06630 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.3e-15
Identity = 102/295 (34.58%), Postives = 124/295 (42.03%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGNM---IVKKR---------- 60
           MTKS   S+V    + S NLH+FLKTKRRI+K AK++ P N     +K            
Sbjct: 1   MTKSCVVSQV----QISGNLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTI 60

Query: 61  --------CGSGFGSGSGSGLVSLA-KNRA-LCNLYSNLPIHIVVKSSSMEFSTS----- 120
                    GS     + S LV LA KNRA + N  SNLP   +VKSS ME S+S     
Sbjct: 61  EEFHGSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEK 120

Query: 121 -----KGRIT------------------------------------LRFGSNGLP----- 180
                 G  T                                    L+FG NGL      
Sbjct: 121 EVLSNLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVR 180

Query: 181 RLMKDILRGLSNIHC-------DIKPRNMLLLPGGRGIETDSSSADFGLGKKF------- 191
           R  +DI+RGL  +HC       DIK RN+LL  G       +  A FGL +K        
Sbjct: 181 RYTRDIVRGLYYMHCNGRYIHGDIKSRNILLSHG------MAKLASFGLARKLTAEVICE 240

BLAST of Moc06g06630 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.6e-13
Identity = 95/295 (32.20%), Postives = 124/295 (42.03%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGN----------------MIV 60
           MTKS   S V    ++  NLH+FLKTKRRI+K AK++ P N                  +
Sbjct: 1   MTKSCVVSRV----QFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  KKRCGSGFGS-----GSGSGLVSLA-KNRA-LCNLYSNLPIHIVVKSSSMEFSTS----- 120
           ++  GS +        + S L+ LA KNRA + N  SNLP   +VKSS  E S+S     
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 ----------------KGRITL-------------------------RFGSNGLP----- 180
                              IT+                         +FG NGL      
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 RLMKDILRGLSNIHC-------DIKPRNMLLLPGGRGIETDSSSADFGLGKKF------- 191
           +  +DI+ GL  +HC       DIK RN+LL  G       +  A FGL +K        
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG------MAKLASFGLARKLTAEVICE 240

BLAST of Moc06g06630 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.6e-13
Identity = 95/295 (32.20%), Postives = 124/295 (42.03%), Query Frame = 0

Query: 1   MTKSSDASEVNEPNRYSFNLHDFLKTKRRIVKCAKRTKPGN----------------MIV 60
           MTKS   S V    ++  NLH+FLKTKRRI+K AK++ P N                  +
Sbjct: 1   MTKSCVVSRV----QFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  KKRCGSGFGS-----GSGSGLVSLA-KNRA-LCNLYSNLPIHIVVKSSSMEFSTS----- 120
           ++  GS +        + S L+ LA KNRA + N  SNLP   +VKSS  E S+S     
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 ----------------KGRITL-------------------------RFGSNGLP----- 180
                              IT+                         +FG NGL      
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 RLMKDILRGLSNIHC-------DIKPRNMLLLPGGRGIETDSSSADFGLGKKF------- 191
           +  +DI+ GL  +HC       DIK RN+LL  G       +  A FGL +K        
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG------MAKLASFGLARKLTAEVICE 240

BLAST of Moc06g06630 vs. TAIR 10
Match: AT2G34290.1 (Protein kinase superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 3.7e-10
Identity = 43/131 (32.82%), Postives = 57/131 (43.51%), Query Frame = 0

Query: 99  RFGSNGLP-----RLMKDILRGLSNI------HCDIKPRNMLLLPGGRGIETDSSSADFG 158
           RF    LP     +  + +L GL+ I      HCD+KP N+L+ PG    +     +DFG
Sbjct: 96  RFNDKKLPDPMIRKFTRMLLEGLATIHRHGYVHCDLKPENILVFPGS-VCDLKLKISDFG 155

Query: 159 LGKK----------------------FEIS----GRPADIWAFGCVVLEMLTGKRPEWRF 193
           L K+                        IS    G+  D+W+ GCVVLEM TGKRP W  
Sbjct: 156 LSKRDGDTTWWHPLKSYAGTPIYMSPESISHGEIGKGLDLWSLGCVVLEMYTGKRPWWHT 215

BLAST of Moc06g06630 vs. TAIR 10
Match: AT2G05060.1 (Protein kinase superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.2e-08
Identity = 31/94 (32.98%), Postives = 43/94 (45.74%), Query Frame = 0

Query: 112 ILRGLSNIHCDIKPRNMLLLPGGRGIETDSSSADFGLGKK----------FEISGRPA-- 171
           ++ G   +HCD+KP N+L+ P       +   +DFGL K+             +G P   
Sbjct: 135 VIHGHGYVHCDLKPENILVFP-----SFELKISDFGLSKREGDSKWWLPSHPFAGTPVYM 194

Query: 172 --------------DIWAFGCVVLEMLTGKRPEW 180
                         D+W+ GCVVLEM TGKRP W
Sbjct: 195 SPESISNGETRRGLDLWSLGCVVLEMYTGKRPWW 223

BLAST of Moc06g06630 vs. TAIR 10
Match: AT5G27790.1 (Protein kinase superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.2e-08
Identity = 48/151 (31.79%), Postives = 62/151 (41.06%), Query Frame = 0

Query: 87  MEFST--SKGRITLRFGSNGLP-----RLMKDILRGLSNI------HCDIKPRNMLLLPG 146
           ME++T  S      RF    LP     +  + +L GL+ I      H DIKP N+L+ PG
Sbjct: 101 MEYATGGSLNNFMDRFNDRKLPDPMIRKFTRMLLEGLATIHRYGYVHYDIKPENILVFPG 160

Query: 147 GRGIE------TDSSSADFGLGKK----------------------FEIS----GRPADI 193
               E           +DFGL K+                        IS    G+  D+
Sbjct: 161 SVYKEGAWRYSYKLKISDFGLSKRDGDTKWWHPLKSYAGTRIYMSPESISHGEIGKGLDL 220

BLAST of Moc06g06630 vs. TAIR 10
Match: AT2G41910.1 (Protein kinase superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 6.0e-08
Identity = 33/113 (29.20%), Postives = 48/113 (42.48%), Query Frame = 0

Query: 113 LRGLSNIHCDIKPRNMLLLP------GGRGIETDSSSADFGLGKKFEIS----------- 172
           + G   +HCD+KP N+L+ P             +   +DFGL K+   S           
Sbjct: 126 IHGQGYVHCDLKPDNILVFPRCVYKKRAWRSSYELKISDFGLSKRDGDSKWWHPHRPFVG 185

Query: 173 ---------------GRPADIWAFGCVVLEMLTGKRPEWRFGNSYEHLLDYYS 194
                          GR  D+W+ GCVVLEM TGK+P W      + L ++Y+
Sbjct: 186 TAIYMSPGSVSHGETGRGLDLWSLGCVVLEMYTGKKPWWHNNYDLKDLKNWYA 238

BLAST of Moc06g06630 vs. TAIR 10
Match: AT2G30040.1 (mitogen-activated protein kinase kinase kinase 14 )

HSP 1 Score: 54.3 bits (129), Expect = 1.3e-07
Identity = 29/84 (34.52%), Postives = 41/84 (48.81%), Query Frame = 0

Query: 119 IHCDIKPRNMLLLPGGRGIETDSSSADFGLGKKFEIS----------------------- 178
           +HCD+K +N+L+  GG  ++     ADFG   +FE S                       
Sbjct: 137 VHCDVKSKNVLVFNGGSSVKL----ADFGSAVEFEKSTIHVSPRGSPLWMAPEVVRREYQ 196

Query: 179 GRPADIWAFGCVVLEMLTGKRPEW 180
           G  +D+W+ GC V+EMLTGK P W
Sbjct: 197 GPESDVWSLGCTVIEMLTGK-PAW 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144818.11.9e-112100.00dual specificity tyrosine-phosphorylation-regulated kinase 3-like [Momordica cha... [more]
KAG6589837.11.5e-2135.37Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper... [more]
XP_022960664.17.6e-2134.14mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
KAG7023508.11.3e-2035.03Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
XP_023516554.16.4e-2035.37mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
O808881.2e-0531.43Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9SND62.1e-0534.78Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O437814.6e-0527.74Dual specificity tyrosine-phosphorylation-regulated kinase 3 OS=Homo sapiens OX=... [more]
Q4R6S54.6e-0527.74Dual specificity tyrosine-phosphorylation-regulated kinase 3 OS=Macaca fascicula... [more]
Q922Y06.0e-0529.10Dual specificity tyrosine-phosphorylation-regulated kinase 3 OS=Mus musculus OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1CUA99.1e-113100.00dual specificity tyrosine-phosphorylation-regulated kinase 3-like OS=Momordica c... [more]
A0A6J1HBP53.7e-2134.14mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A0A0LHV12.3e-1534.58Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829... [more]
A0A5D3D3K91.6e-1332.20Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma... [more]
A0A1S3B8291.6e-1332.20mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT2G34290.13.7e-1032.82Protein kinase superfamily protein [more]
AT2G05060.11.2e-0832.98Protein kinase superfamily protein [more]
AT5G27790.11.2e-0831.79Protein kinase superfamily protein [more]
AT2G41910.16.0e-0829.20Protein kinase superfamily protein [more]
AT2G30040.11.3e-0734.52mitogen-activated protein kinase kinase kinase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 98..202
e-value: 7.4E-13
score: 50.1
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 110..153
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 157..183
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 157..183
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 110..153
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..204
score: 8.570164
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 107..194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g06630.1Moc06g06630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity