Moc06g05880 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g05880
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionomega-6 fatty acid desaturase, chloroplastic
Locationchr6: 4244642 .. 4248494 (-)
RNA-Seq ExpressionMoc06g05880
SyntenyMoc06g05880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTGCAGACTTGCGGATTCCATGTTTCTTTTCACTGTACGTTTCCCGCCATCTAGTTCTTATTTCGCCCTTTTTGGTGCTTCTGCTTCTGTTTCTTATTGTTTTAGTTTGATTATCAGGGCCCCCATCAGAATTCAGCTCGAACACAAAAAACTGCATCCCATTATTCTCCGGGTATGTTTGAGTATCTTCCTTTTGAGTTCTAAGCCATTGAATTATGTGAAGTTGAAGTGTTGGCATTCACTGTGTGAGAGTTCATGCATTGGAGATTGAATTCCATTTACTTTCCTACAAATCATCTCAAGCATGAACTTTATATACAAAATAATGAATTAACTGACGAGGAACCGACCGAACTAGCTTGGATTTTGCATTGCTTACATTATCCTCATTTTGGTTTGTTCTAAGTTTTGCATTGTATTCTTTAACTTCTTTTTTGCCCTGCTGTCTCTTTGAGCATAACAACTTAGGTTTTCCATTTTCAATTTAGATGCTTCAATTCAAACATGACTTTTGCAAGACAGGTATATTGCACGTGAAGGGGGAAATAATATTCCAGAAGAAAATCAAGCGACGAAACTATCTACTTCCCGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCTGTTCCAGTTGCACCACCTACTTCAGCAGATAGTTCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCGAGAAAATTGGAGAACCTCTTCCTGAAAATGTTAGGTTGAAAGATATTGTTGAGTCCCTTCCAAAAGAGGTACTTCATTTGCCGCAACATTATAGTGTTGGCATTCATCCCGAGACAGTTCATGTTTTTTTTTTTAATTGGGTCAAATAAAATGTTGATGATGTTCTAGTTCATGTTTATAGTTGCTTACATAGCCTTCCAGCCTTTGGGTGAGGGAGGTTGTGAGTGGACAATGCTTTCTCTCTTCCCCATTTTTCCGTTGCTCACTAGTCGAGCAAAAACTTCAAGCATTTAAATTCTTCATATCCTCGAAGTCTTCTCGTGAGAAGATGCGACTAAAAAAGGTAGTGCCATTTTTGCTCATTTTATCTTATTCTTTTTGACTTTTAAGGTGTTCGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTATACATTAGGTCTTATCATGATTGCAAAAGCTCCATGGTATTTACTACCTTTGGCTTGGGCATGGACTGGGACTGCAATAACAGGGGTTAGTCTTATTCAATTTTATTTCTATTTGCTTCTCTATCTCCCTCCCATTCTTGTTGGAGTTTGTAACGTAGCTCCCTGAAGTTATTTTTTCTTTCTTTTCCCCTCAAGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAAGAACAAGTTAGTGGAAGACATCGTGGGTACTTTGGCTTTCCTACCTTTAATATACCCATACGAACCTTGGCGGTTTAAGCACGATCAACATCATGCGAAGACAAACATGTAGAAAATCTCTCCTTTTCTTTTTTTCTCTCTCTCTCTTCAAAATACTTATTTTAGCACTATCTTCCTGCATAAGCATAGTTAAATGTTATTTCAAGGTATAGTATGAACAAGTGTCTTGTTGAGCAGAACAACATGAATGGATTTTAATGTACCATAATCCGTAGCTTGACACTGCTAGGTCTACACACATGGCATCTTATTTGCTGGACACTGCTGGGCGTGCAGTAATTCTTCCTAGTATCTTTGAATCAAGTTAATAGAATTTCTCTATATGTTTTGCATTTTGCTTAGAGTCAAATATCTTATGCGTTAATTAAGCTTAAAAAACTAAATGTATGACTATGATACATAGAAATCAATATCAGGATGGCTTTTATTTTCAACGTAGGCATCAAAGTTTTAGGTTATTTTTATAATTTTGGCCGGTTACCTACATCTTAAAGATTTCTGCCTTTCTTCTTTTATAAGCTGAAGTTCCATTTATATTCCTATCTCAATATATAGGCTGAAAGAAGATACGGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGTACCTCTTATGCGTAAAGCAATCATATATGGATATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTAAGGTACCTTGGATGGTTCCTTGGGGATTATCATTTGTCAAGGATTGCAAGAATAACCTGACTTCATATCAAGATATCCTGTATGTGAATTGACCGCTTGCTCTCGCTCTCTTTCACTAATATGCAACAATTTGTTCTGCATAATAGGTTGTTGTGGCACTTCAATTTGAAAATATTCAGACCAAATGAAGTGAAAAGAGTGAAGATAAGCCTAGCTTGCGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGGCTGGGATTCTCGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGCTACCACTTTTGGGTAACATCATCTGCTTGCCTCTTGATATTATATTTTCCCTATGTTCAATTTAACTGATGATCTGCACTGAATGGTGACTTGTCATGGATCTCCATGGTGGAACATTAAATTAACAAACTAGCTCAAAATTAGATAAGAGGATTATATGATGATTGATGCCATAGACACTAACAAGAATGAACTGCGTAACTTAGACCACCGTATCTGAGTATCTTTTGACATTGTTGAAACATAAATCTTAGTGAAGCTTATATCTTGCGAGTTGTATGAATATTACTGATACAAATAAAAAATGATTAATACCATGCTTCCAAGATGATGAAATGATGATCTGATGTTTATGCTGCTATTATTCATTCATGGTGGGTGACTGTTGTTTTGTCATTCTTGTTAAAAACTGCATGATTGTGAAACTAGCCCATATATATATATTCTTTTCACTAAAAGTCTCACTAAGGAAGATTGTTGTTTGTGGATGGGACGATCTACATCTATCTTTTGGTTATTGTTTGGCGTTTGCCTACTTATGGTTTTGTATCTAAGAATTACCTTTTATTTCTTGCTTAGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCATTTAAATCTTCAGAAGAGTGGAATGCAGCCCAAGCACAGCTCAATGGAACGGTTCATTGTGCTTACCCTCAGTGGTATAGATCATCTATTTTAGTTTTTCTTTTTCTCTGTTCTCTTCCTCAGTACTTTACTCATTTGTTTCTTAATTTAATATTGCTAATCTAAAAATTCTCTCATTGTTGGGCAAGTACTAAGCAGTTAAGGTGCTGTTTAGGTGTGTTTTCTGTAGATTAGTTTCTTAGAATGTTTTATGAAGCCATGTTAAAAAGAGATTCTTGCACTGTTCATGAGTTTCCTTTACCATCAGCCAGTTAACCGCGAATATAATCTCTTCCCATGTAGGATCGAGATTCTATGCCACGACATCAATGTGCACATCCCCCACCACATTTCTCCAAGAATCCCAAGTTACAACTTACGTGCAGCCCACAAGTCTCTTCAAGAGAACTGGGGAAAGGTAATGGCCTGAACCGAATGCATGTCTGCGTTTTTCTTTTAGACACATTTTGGATTTCAGTTCTTGAAAGGTTAATGCTGCCTTGCATTGCATCTATTCTCCTCCCATTGATGCTAACAGTCTAATTCTTTCGTTTCATATTTGTTTTCCCTCCAACTCCTTCTATAGTATCTGAACGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATGTGCCATGTCTACAGTAAAGAGGAAAACTACGTCGCTTTTGACCGACTCAACCCAGAAGATTCTTATCCCATTACATTCCTCAGGAAAGTTATGCCTGATATTGCTTGA

mRNA sequence

ATGGCTTGCAGACTTGCGGATTCCATGTTTCTTTTCACTGGCCCCCATCAGAATTCAGCTCGAACACAAAAAACTGCATCCCATTATTCTCCGGGTATATTGCACGTGAAGGGGGAAATAATATTCCAGAAGAAAATCAAGCGACGAAACTATCTACTTCCCGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCTGTTCCAGTTGCACCACCTACTTCAGCAGATAGTTCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCGAGAAAATTGGAGAACCTCTTCCTGAAAATGTTAGGTTGAAAGATATTGTTGAGTCCCTTCCAAAAGAGGTGTTCGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTATACATTAGGTCTTATCATGATTGCAAAAGCTCCATGGTATTTACTACCTTTGGCTTGGGCATGGACTGGGACTGCAATAACAGGGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAAGAACAAGTTAGTGGAAGACATCGTGGGTACTTTGGCTTTCCTACCTTTAATATACCCATACGAACCTTGGCGGTTTAAGCACGATCAACATCATGCGAAGACAAACATGCTGAAAGAAGATACGGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGTACCTCTTATGCGTAAAGCAATCATATATGGATATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTCAATTTGAAAATATTCAGACCAAATGAAGTGAAAAGAGTGAAGATAAGCCTAGCTTGCGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGGCTGGGATTCTCGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCATTTAAATCTTCAGAAGAGTGGAATGCAGCCCAAGCACAGCTCAATGGAACGGTTCATTGTGCTTACCCTCAGTGGATCGAGATTCTATGCCACGACATCAATGTGCACATCCCCCACCACATTTCTCCAAGAATCCCAAGTTACAACTTACGTGCAGCCCACAAGTCTCTTCAAGAGAACTGGGGAAAGTATCTGAACGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATGTGCCATGTCTACAGTAAAGAGGAAAACTACGTCGCTTTTGACCGACTCAACCCAGAAGATTCTTATCCCATTACATTCCTCAGGAAAGTTATGCCTGATATTGCTTGA

Coding sequence (CDS)

ATGGCTTGCAGACTTGCGGATTCCATGTTTCTTTTCACTGGCCCCCATCAGAATTCAGCTCGAACACAAAAAACTGCATCCCATTATTCTCCGGGTATATTGCACGTGAAGGGGGAAATAATATTCCAGAAGAAAATCAAGCGACGAAACTATCTACTTCCCGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCTGTTCCAGTTGCACCACCTACTTCAGCAGATAGTTCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCGAGAAAATTGGAGAACCTCTTCCTGAAAATGTTAGGTTGAAAGATATTGTTGAGTCCCTTCCAAAAGAGGTGTTCGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTATACATTAGGTCTTATCATGATTGCAAAAGCTCCATGGTATTTACTACCTTTGGCTTGGGCATGGACTGGGACTGCAATAACAGGGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAAGAACAAGTTAGTGGAAGACATCGTGGGTACTTTGGCTTTCCTACCTTTAATATACCCATACGAACCTTGGCGGTTTAAGCACGATCAACATCATGCGAAGACAAACATGCTGAAAGAAGATACGGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGTACCTCTTATGCGTAAAGCAATCATATATGGATATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTCAATTTGAAAATATTCAGACCAAATGAAGTGAAAAGAGTGAAGATAAGCCTAGCTTGCGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGGCTGGGATTCTCGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCATTTAAATCTTCAGAAGAGTGGAATGCAGCCCAAGCACAGCTCAATGGAACGGTTCATTGTGCTTACCCTCAGTGGATCGAGATTCTATGCCACGACATCAATGTGCACATCCCCCACCACATTTCTCCAAGAATCCCAAGTTACAACTTACGTGCAGCCCACAAGTCTCTTCAAGAGAACTGGGGAAAGTATCTGAACGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATGTGCCATGTCTACAGTAAAGAGGAAAACTACGTCGCTTTTGACCGACTCAACCCAGAAGATTCTTATCCCATTACATTCCTCAGGAAAGTTATGCCTGATATTGCTTGA

Protein sequence

MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAILRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGYGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDIA
Homology
BLAST of Moc06g05880 vs. NCBI nr
Match: XP_022135232.1 (omega-6 fatty acid desaturase, chloroplastic [Momordica charantia])

HSP 1 Score: 937.9 bits (2423), Expect = 3.1e-269
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI
Sbjct: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVK 120
           LRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVK
Sbjct: 61  LRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVK 120

Query: 121 AWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVE 180
           AWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVE
Sbjct: 121 AWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVE 180

Query: 181 DIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGY 240
           DIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGY
Sbjct: 181 DIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGY 240

Query: 241 GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKF 300
           GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKF
Sbjct: 241 GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKF 300

Query: 301 WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDIN 360
           WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDIN
Sbjct: 301 WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDIN 360

Query: 361 VHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAF 420
           VHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAF
Sbjct: 361 VHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAF 420

Query: 421 DRLNPEDSYPITFLRKVMPDIA 443
           DRLNPEDSYPITFLRKVMPDIA
Sbjct: 421 DRLNPEDSYPITFLRKVMPDIA 442

BLAST of Moc06g05880 vs. NCBI nr
Match: XP_023529482.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889.4 bits (2297), Expect = 1.3e-254
Identity = 412/443 (93.00%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADS+FLFTGPHQN ARTQK ASH +PGIL VKG+I+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPP +SAD +EYRKQLSESYGFE+IGEPLPENVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASYTLGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDS PL+RKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVH+PHHISPRIPSYNLRAAHKSLQENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHVPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRL PEDSYPITFLRKVMPD+A
Sbjct: 421 FDRLKPEDSYPITFLRKVMPDLA 443

BLAST of Moc06g05880 vs. NCBI nr
Match: XP_022930407.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 889.0 bits (2296), Expect = 1.6e-254
Identity = 413/443 (93.23%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADS+FLFTGPHQN ARTQK ASH +PGIL VKG+I+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPP +SAD +EYRKQLSESYGFE+IGEPLPENVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDS PL+RKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRL PEDSYPITFL+KVMPD+A
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of Moc06g05880 vs. NCBI nr
Match: XP_038880078.1 (omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-253
Identity = 414/443 (93.45%), Postives = 431/443 (97.29%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADSM LF GPHQN ARTQK ASH SPGIL VKGEI+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPP +SADS+EYRKQLSESYGFE+IGEPLP+NVRLKDIVESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASYTLGL+MIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDS PL+RKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRLN EDSYPITFL+KVMPD+A
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Moc06g05880 vs. NCBI nr
Match: XP_022965421.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 884.4 bits (2284), Expect = 4.0e-253
Identity = 410/443 (92.55%), Postives = 432/443 (97.52%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADS+FLFTGPHQN ARTQK ASH +PGIL VKG+I+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPP +SAD +EYRKQLSESYGFE+IGEPLPENVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASYTLGL++IAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVVIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDS PL+RKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSL+ENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLKENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRL PEDSYPI FLRKVMPD+A
Sbjct: 421 FDRLKPEDSYPIKFLRKVMPDLA 443

BLAST of Moc06g05880 vs. ExPASy Swiss-Prot
Match: P48627 (Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 3.4e-210
Identity = 343/444 (77.25%), Postives = 385/444 (86.71%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTAS-HYSPGILHVKGEIIFQKKIKRRNYLLPV-KRA 60
           MA R+ADS+F FTGP Q   R  K AS   SPG+  V+  I    K  RR +L+P  KR 
Sbjct: 1   MASRIADSLFAFTGPQQCLPRAPKLASARLSPGVYAVR-PIDLLLKGTRRTFLVPAKKRI 60

Query: 61  AILRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEIND 120
             ++AV VPVAPP SAD++E R+QL+ESYGF++IG+ LP+NV LKDI+++LPKEVFEI+D
Sbjct: 61  GCIKAVFVPVAPP-SADNAEDREQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDD 120

Query: 121 VKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKL 180
           VKAWKSVL+SV SY LGL MIAKAPWYLLPLAWAWTGTA+TGFFVIGHDCAHKSFSKNKL
Sbjct: 121 VKAWKSVLISVTSYALGLFMIAKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSKNKL 180

Query: 181 VEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIY 240
           VEDIVGTLAFLPL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEFDS P++RKAII+
Sbjct: 181 VEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFDSSPVLRKAIIF 240

Query: 241 GYGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWV 300
           GYGP RPW+SIAHW+ WHFNL+ FRP+EV RVKISLACVFAFM +GWPLIIYK G+LGWV
Sbjct: 241 GYGPIRPWLSIAHWVNWHFNLRKFRPSEVNRVKISLACVFAFMAVGWPLIIYKVGVLGWV 300

Query: 301 KFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHD 360
           KFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEILCHD
Sbjct: 301 KFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEILCHD 360

Query: 361 INVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYV 420
           INVHIPHHISPRIPSYNLRAAH+S+QENWGKY N A+WNWRLMKTIMT+CHVY KEENY+
Sbjct: 361 INVHIPHHISPRIPSYNLRAAHQSIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEENYI 420

Query: 421 AFDRLNPEDSYPITFLRKVMPDIA 443
            FDRL PE+S PITFL+K MPD A
Sbjct: 421 PFDRLAPEESQPITFLKKAMPDYA 442

BLAST of Moc06g05880 vs. ExPASy Swiss-Prot
Match: P46312 (Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD6 PE=1 SV=2)

HSP 1 Score: 720.7 bits (1859), Expect = 1.0e-206
Identity = 341/446 (76.46%), Postives = 384/446 (86.10%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTA---SHYSPGILHVKG-EIIFQKKIKR-RNYLLPV 60
           MA R+ADS+F FTGP Q   R  K A   +  SPG+  VK  +++ + +  R R  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 61  K-RAAILRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVF 120
           K R   ++AVA PVAPP SADS+E R+QL+ESYGF +IGE LPENV LKDI+++LPKEVF
Sbjct: 61  KRRIGCIKAVAAPVAPP-SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVF 120

Query: 121 EINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS 180
           EI+D+KA KSVL+SV SYTLGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS
Sbjct: 121 EIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS 180

Query: 181 KNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRK 240
           KNKLVEDIVGTLAFLPL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S P+MRK
Sbjct: 181 KNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMRK 240

Query: 241 AIIYGYGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGI 300
           AII+GYGP RPW+SIAHW+ WHFNLK FR +EV RVKISLACVFAFM +GWPLI+YK GI
Sbjct: 241 AIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVGI 300

Query: 301 LGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEI 360
           LGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEI
Sbjct: 301 LGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEI 360

Query: 361 LCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKE 420
           LCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGKY N A+WNWRLMKTIMT+CHVY KE
Sbjct: 361 LCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDKE 420

Query: 421 ENYVAFDRLNPEDSYPITFLRKVMPD 441
           ENY+ FDRL PE+S PITFL+K MP+
Sbjct: 421 ENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of Moc06g05880 vs. ExPASy Swiss-Prot
Match: P48628 (Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 3.7e-201
Identity = 317/425 (74.59%), Postives = 362/425 (85.18%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MAC LADS+ LF G +Q     +  A+ YSPGI  +    + QK+ +R+   +   +  +
Sbjct: 1   MACTLADSLLLFKGSYQKPVLRRDIAARYSPGIFSLNSNGLIQKRFRRQRNFVTRNKVTV 60

Query: 61  LRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVK 120
           + AVA+PV  P   +S+EYRKQL+E YGF ++GEPL ++V LKD++  LPKEVFEI+DVK
Sbjct: 61  IHAVAIPV-QPAPVESAEYRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDVK 120

Query: 121 AWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVE 180
           AWKSVL+SV SY LGL MI+KAPWYLLPLAW WTGTAITGFFVIGHDCAH+SFS NKLVE
Sbjct: 121 AWKSVLISVTSYALGLFMISKAPWYLLPLAWVWTGTAITGFFVIGHDCAHRSFSSNKLVE 180

Query: 181 DIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGY 240
           DIVGTLAF+PLIYPYEPWRFKHD+HHAKTNML+EDTAWHPVWK+EF+S PL+RKAIIYGY
Sbjct: 181 DIVGTLAFMPLIYPYEPWRFKHDRHHAKTNMLREDTAWHPVWKDEFESTPLLRKAIIYGY 240

Query: 241 GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKF 300
           GPFR WMSIAHWL+WHF+LK FRP+EV RVKISLACVFAF+ IGWPLIIYK GI+GW+KF
Sbjct: 241 GPFRCWMSIAHWLMWHFDLKKFRPSEVPRVKISLACVFAFIAIGWPLIIYKTGIMGWIKF 300

Query: 301 WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDIN 360
           WLMPWLGYHFWMSTFTMVHHTAP+IPFK SEEWN AQAQLNGTVHC YP+WIEILCHDIN
Sbjct: 301 WLMPWLGYHFWMSTFTMVHHTAPYIPFKYSEEWNRAQAQLNGTVHCDYPKWIEILCHDIN 360

Query: 361 VHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAF 420
           VHIPHHISPRIPSYNLRAAHKSLQENWG+YLNEASWNWRLMKTIMT+C VY KE++    
Sbjct: 361 VHIPHHISPRIPSYNLRAAHKSLQENWGQYLNEASWNWRLMKTIMTVCQVYDKEKSLCCL 420

Query: 421 DRLNP 426
            R  P
Sbjct: 421 RRTCP 424

BLAST of Moc06g05880 vs. ExPASy Swiss-Prot
Match: P48629 (Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD6 PE=1 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 7.0e-184
Identity = 290/390 (74.36%), Postives = 343/390 (87.95%), Query Frame = 0

Query: 56  KRAAILRAVAVPVAPPTS---ADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKE 115
           +++ +++AVAVPVA P++    D++E+ KQL+E YGF++IGEPLP++V ++DI+ SLPK+
Sbjct: 58  RKSTLVQAVAVPVAQPSAFPPTDNTEHLKQLAERYGFQQIGEPLPDDVTMRDIITSLPKQ 117

Query: 116 VFEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 175
           VFEIND KAW +VL+SV SY LG+ MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS
Sbjct: 118 VFEINDTKAWGTVLISVTSYALGIFMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 177

Query: 176 FSKNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLM 235
           FSKNKLVEDIVGTLAF+PLIYPYEPWRFKHDQHH KTNML+EDTAW P+ KE+ +S P +
Sbjct: 178 FSKNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHTKTNMLREDTAWLPIMKEDIESSPGL 237

Query: 236 RKAIIYGYGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKA 295
           RKA+IY YGP R WMSIAHWL  HFNLK FR +EVKR  ISLA VFAFM IGWPLIIYK 
Sbjct: 238 RKALIYAYGPLRTWMSIAHWLKVHFNLKDFRQSEVKRATISLAAVFAFMVIGWPLIIYKT 297

Query: 296 GILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWI 355
           GI+GW+KFWLMPWLGYHFWMSTFT+VHHTAPHIPFKSS+EWNAAQAQL+GTVHC YP+WI
Sbjct: 298 GIVGWIKFWLMPWLGYHFWMSTFTIVHHTAPHIPFKSSKEWNAAQAQLSGTVHCDYPRWI 357

Query: 356 EILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYS 415
           EILCHDI+VHIPHHISP+IPSYNLRAA++SL ENWG+YLN+   NWRLM+TIMT CH+Y 
Sbjct: 358 EILCHDISVHIPHHISPKIPSYNLRAANQSLNENWGEYLNKPKSNWRLMRTIMTTCHIYD 417

Query: 416 KEENYVAFDRLNPEDSYPITFLRKVMPDIA 443
           K+ NYV+F++  PE+S PI+  ++VMPD A
Sbjct: 418 KDGNYVSFEKAVPEESQPISIPKRVMPDYA 447

BLAST of Moc06g05880 vs. ExPASy Swiss-Prot
Match: P20388 (Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=desA PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.6e-106
Identity = 173/326 (53.07%), Postives = 225/326 (69.02%), Query Frame = 0

Query: 99  NVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAI 158
           +++L+DI+++LPKE FE    KAW SVL+++ +  +G + I   PWY LP+ W WTGTA+
Sbjct: 23  DLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPWYCLPITWIWTGTAL 82

Query: 159 TGFFVIGHDCAHKSFSKNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAW 218
           TG FV+GHDC H+SF+K + V D+VG +AF PLIYP+  WR  HD HH  TN ++ D AW
Sbjct: 83  TGAFVVGHDCGHRSFAKKRWVNDLVGHIAFAPLIYPFHSWRLLHDHHHLHTNKIEVDNAW 142

Query: 219 HPVWKEEFDSVPLMRKAIIYGY-GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACV 278
            P   E F + P + +       GPF    SI HW L HF L  F   +  +VK+S+A V
Sbjct: 143 DPWSVEAFQASPAIVRLFYRAIRGPFWWTGSIFHWSLMHFKLSNFAQRDRNKVKLSIAVV 202

Query: 279 FAFMFIGWPLIIYKAGILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQ 338
           F F  I +P +I   G+ G+VKFWLMPWL YHFWMSTFT+VHHT P I F+ + +W+AA+
Sbjct: 203 FLFAAIAFPALIITTGVWGFVKFWLMPWLVYHFWMSTFTIVHHTIPEIRFRPAADWSAAE 262

Query: 339 AQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWN 398
           AQLNGTVHC YP+W+E+LCHDINVHIPHH+S  IPSYNLR AH SL+ENWG +L E ++N
Sbjct: 263 AQLNGTVHCDYPRWVEVLCHDINVHIPHHLSVAIPSYNLRLAHGSLKENWGPFLYERTFN 322

Query: 399 WRLMKTIMTMCHVYSKEENYVAFDRL 424
           W+LM+ I   CH+Y  E  Y  F  L
Sbjct: 323 WQLMQQISGQCHLYDPEHGYRTFGSL 348

BLAST of Moc06g05880 vs. ExPASy TrEMBL
Match: A0A6J1C240 (omega-6 fatty acid desaturase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007246 PE=4 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 1.5e-269
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI
Sbjct: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVK 120
           LRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVK
Sbjct: 61  LRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVK 120

Query: 121 AWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVE 180
           AWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVE
Sbjct: 121 AWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVE 180

Query: 181 DIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGY 240
           DIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGY
Sbjct: 181 DIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGY 240

Query: 241 GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKF 300
           GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKF
Sbjct: 241 GPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKF 300

Query: 301 WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDIN 360
           WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDIN
Sbjct: 301 WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDIN 360

Query: 361 VHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAF 420
           VHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAF
Sbjct: 361 VHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAF 420

Query: 421 DRLNPEDSYPITFLRKVMPDIA 443
           DRLNPEDSYPITFLRKVMPDIA
Sbjct: 421 DRLNPEDSYPITFLRKVMPDIA 442

BLAST of Moc06g05880 vs. ExPASy TrEMBL
Match: A0A6J1EQE5 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436860 PE=4 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 7.9e-255
Identity = 413/443 (93.23%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADS+FLFTGPHQN ARTQK ASH +PGIL VKG+I+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPP +SAD +EYRKQLSESYGFE+IGEPLPENVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDS PL+RKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRL PEDSYPITFL+KVMPD+A
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of Moc06g05880 vs. ExPASy TrEMBL
Match: A0A6J1HLM6 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111465334 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 1.9e-253
Identity = 410/443 (92.55%), Postives = 432/443 (97.52%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRLADS+FLFTGPHQN ARTQK ASH +PGIL VKG+I+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPP +SAD +EYRKQLSESYGFE+IGEPLPENVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASYTLGL++IAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVVIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDS PL+RKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSL+ENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLKENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRL PEDSYPI FLRKVMPD+A
Sbjct: 421 FDRLKPEDSYPIKFLRKVMPDLA 443

BLAST of Moc06g05880 vs. ExPASy TrEMBL
Match: A0A0A0LTB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G255170 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 4.8e-252
Identity = 409/443 (92.33%), Postives = 429/443 (96.84%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRL DSM LF GPH N ARTQK ASH SPGIL VKGEI+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRA+AVP APP +SADS+EYRKQLSESYGFE+IGEPLP+NVRLKD+VESLPKEVFEINDV
Sbjct: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASY+LGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDS PL+RKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GILGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGK+LNEASWNWRLMKTIMT+CHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRLN EDSYPITFL+KVMPD+A
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Moc06g05880 vs. ExPASy TrEMBL
Match: A0A1S3B9S5 (omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487358 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 6.9e-251
Identity = 409/443 (92.33%), Postives = 429/443 (96.84%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60
           MACRL DSM LF GPHQN ARTQK ASH SPG L VKGEI+FQKKIKR+NYLLPVK+AAI
Sbjct: 1   MACRLVDSMALFNGPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAI 60

Query: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPP +SADS+EYRKQLSESYGFE+IGEPLP+NVRLKDIVESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180
           KAWKSVLVSVASY+LGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNML+EDTAWHPV KEEFDS P++RKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GILGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           NVHIPHH+SPRIPSYNLRAAHKSLQENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVA 420

Query: 421 FDRLNPEDSYPITFLRKVMPDIA 443
           FDRLN EDSYPITFL+KVMPD+A
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Moc06g05880 vs. TAIR 10
Match: AT4G30950.1 (fatty acid desaturase 6 )

HSP 1 Score: 720.7 bits (1859), Expect = 7.2e-208
Identity = 341/446 (76.46%), Postives = 384/446 (86.10%), Query Frame = 0

Query: 1   MACRLADSMFLFTGPHQNSARTQKTA---SHYSPGILHVKG-EIIFQKKIKR-RNYLLPV 60
           MA R+ADS+F FTGP Q   R  K A   +  SPG+  VK  +++ + +  R R  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 61  K-RAAILRAVAVPVAPPTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVF 120
           K R   ++AVA PVAPP SADS+E R+QL+ESYGF +IGE LPENV LKDI+++LPKEVF
Sbjct: 61  KRRIGCIKAVAAPVAPP-SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVF 120

Query: 121 EINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS 180
           EI+D+KA KSVL+SV SYTLGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS
Sbjct: 121 EIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS 180

Query: 181 KNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRK 240
           KNKLVEDIVGTLAFLPL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S P+MRK
Sbjct: 181 KNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMRK 240

Query: 241 AIIYGYGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGI 300
           AII+GYGP RPW+SIAHW+ WHFNLK FR +EV RVKISLACVFAFM +GWPLI+YK GI
Sbjct: 241 AIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVGI 300

Query: 301 LGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEI 360
           LGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEI
Sbjct: 301 LGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEI 360

Query: 361 LCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKE 420
           LCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGKY N A+WNWRLMKTIMT+CHVY KE
Sbjct: 361 LCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDKE 420

Query: 421 ENYVAFDRLNPEDSYPITFLRKVMPD 441
           ENY+ FDRL PE+S PITFL+K MP+
Sbjct: 421 ENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of Moc06g05880 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 133.3 bits (334), Expect = 4.9e-31
Identity = 87/334 (26.05%), Postives = 161/334 (48.20%), Query Frame = 0

Query: 101 RLKDIVESLPKEVFEINDVKAWKSV---LVSVASYTLGLIMIAKAPWYLLPLAWAWTGTA 160
           ++ DI  ++PK  +  + +++   V   +++VA+  +  + +    W+L PL WA  GT 
Sbjct: 35  KIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALAIAAVYVDS--WFLWPLYWAAQGTL 94

Query: 161 ITGFFVIGHDCAHKSFSKNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTA 220
               FV+GHDC H SFS   L+  +VG +    ++ PY  WR  H  HH     ++ D +
Sbjct: 95  FWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHGHVENDES 154

Query: 221 WHPVWKEEFDSVPLMRKAIIYGYGPFRPWMSIAHWLLW--------HFN--LKIFRPNEV 280
           W P+ +  +  +P   + + Y      P ++   +L +        HFN    +F P+E 
Sbjct: 155 WVPLPERVYKKLPHSTRMLRYTVP--LPMLAYPLYLCYRSPGKEGSHFNPYSSLFAPSER 214

Query: 281 KRVKISLACVFAFMFIGWPLIIYKAGILGWVKFWLMPWLGYHFWMSTFTMVHHTA--PHI 340
           K +  S  C ++ MF+    + +  G L  +K + +P++ +  W+   T +HH      +
Sbjct: 215 KLIATSTTC-WSIMFVSLIALSFVFGPLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKL 274

Query: 341 PFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQE 400
           P+   +EW+  +  L  T+   Y  +  I  HDI  H+ HH+ P+IP Y+L  A K+ + 
Sbjct: 275 PWYRGKEWSYLRGGLT-TIDRDYGIFNNI-HHDIGTHVIHHLFPQIPHYHLVDATKAAKH 334

Query: 401 NWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
             G+Y  E   +  +   ++       K+++YV+
Sbjct: 335 VLGRYYREPKTSGAIPIHLVESLVASIKKDHYVS 361

BLAST of Moc06g05880 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-29
Identity = 96/344 (27.91%), Postives = 156/344 (45.35%), Query Frame = 0

Query: 93  GEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAKAP----WYLLP 152
           G P P N  L DI  ++PK  +  N    WKS+   V    +   + A A     W + P
Sbjct: 91  GAPPPFN--LADIRAAIPKHCWVKN---PWKSLSYVVRDVAIVFALAAGAAYLNNWIVWP 150

Query: 153 LAWAWTGTAITGFFVIGHDCAHKSFSKNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAK 212
           L W   GT     FV+GHDC H SFS +  +  +VG L    ++ PY  WR  H  HH  
Sbjct: 151 LYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQN 210

Query: 213 TNMLKEDTAWHPVWKEEFD-----------SVPLMRKAIIYGYGPFRPWMSIAHWLLWHF 272
              ++ D +WHP+ ++ ++           ++PL+  A  +      P    +H   +H 
Sbjct: 211 HGHVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSH---YHP 270

Query: 273 NLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKFWLMPWLGYHFWMSTFTM 332
           +  +F P E K V  S AC  A   +    + +  G +  +K + +P+     W+   T 
Sbjct: 271 DSDLFLPKERKDVLTSTACWTAMAAL-LVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTY 330

Query: 333 VHHTA--PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYN 392
           +HH      +P+   +EW+  +  L  T+   Y   I  + HDI  H+ HH+ P+IP Y+
Sbjct: 331 LHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GLINNIHHDIGTHVIHHLFPQIPHYH 390

Query: 393 LRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420
           L  A ++ +   GKY  E   +  L   ++ +     KE++YV+
Sbjct: 391 LVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS 423

BLAST of Moc06g05880 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 124.0 bits (310), Expect = 3.0e-28
Identity = 91/315 (28.89%), Postives = 144/315 (45.71%), Query Frame = 0

Query: 93  GEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAK--APWYLLPLA 152
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 153 WAWTGTAITGFFVIGHDCAHKSFSKNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTN 212
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 213 MLKEDTAWHPVWKEEFDSVPLMRKAIIYGYGPFRPWMSIAHWLL----------WHFNLK 272
            ++ D +WHP+ +  + ++    +   +   PF P ++   +L           +H +  
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTL-PF-PMLAYPFYLWNRSPGKQGSHYHPDSD 263

Query: 273 IFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKFWLMPWLGYHFWMSTFTMVHH 332
           +F P E K V  S AC  A   +    + +  G +  +K + +P+  +  W+   T +HH
Sbjct: 264 LFLPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHH 323

Query: 333 TA--PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRA 392
                 +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L  
Sbjct: 324 HGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHLVE 383

Query: 393 AHKSLQENWGKYLNE 394
           A ++ +   GKY  E
Sbjct: 384 ATEAAKPVLGKYYRE 390

BLAST of Moc06g05880 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 117.5 bits (293), Expect = 2.8e-26
Identity = 86/298 (28.86%), Postives = 136/298 (45.64%), Query Frame = 0

Query: 93  GEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAK--APWYLLPLA 152
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 153 WAWTGTAITGFFVIGHDCAHKSFSKNKLVEDIVGTLAFLPLIYPYEPWRFKHDQHHAKTN 212
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 213 MLKEDTAWHPVWKEEFDSVPLMRKAIIYGYGPFRPWMSIAHWLL----------WHFNLK 272
            ++ D +WHP+ +  + ++    +   +   PF P ++   +L           +H +  
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTL-PF-PMLAYPFYLWNRSPGKQGSHYHPDSD 263

Query: 273 IFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKFWLMPWLGYHFWMSTFTMVHH 332
           +F P E K V  S AC  A   +    + +  G +  +K + +P+  +  W+   T +HH
Sbjct: 264 LFLPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHH 323

Query: 333 TA--PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNL 377
                 +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L
Sbjct: 324 HGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHL 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135232.13.1e-269100.00omega-6 fatty acid desaturase, chloroplastic [Momordica charantia][more]
XP_023529482.11.3e-25493.00omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022930407.11.6e-25493.23omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata][more]
XP_038880078.11.1e-25393.45omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida][more]
XP_022965421.14.0e-25392.55omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P486273.4e-21077.25Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1[more]
P463121.0e-20676.46Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P486283.7e-20174.59Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1[more]
P486297.0e-18474.36Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD... [more]
P203882.6e-10653.07Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ... [more]
Match NameE-valueIdentityDescription
A0A6J1C2401.5e-269100.00omega-6 fatty acid desaturase, chloroplastic OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1EQE57.9e-25593.23omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1HLM61.9e-25392.55omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A0A0LTB14.8e-25292.33FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G2551... [more]
A0A1S3B9S56.9e-25192.33omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT4G30950.17.2e-20876.46fatty acid desaturase 6 [more]
AT2G29980.14.9e-3126.05fatty acid desaturase 3 [more]
AT3G11170.11.2e-2927.91fatty acid desaturase 7 [more]
AT5G05580.13.0e-2828.89fatty acid desaturase 8 [more]
AT5G05580.22.8e-2628.86fatty acid desaturase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 144..390
e-value: 5.4E-30
score: 105.1
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 1..442
NoneNo IPR availablePANTHERPTHR32100:SF67BNAC03G67820D PROTEINcoord: 1..442
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 120..376
e-value: 2.99514E-80
score: 245.598

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g05880.1Moc06g05880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane