Moc06g04860 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g04860
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description(DL)-glycerol-3-phosphatase 2
Locationchr6: 3570633 .. 3573763 (-)
RNA-Seq ExpressionMoc06g04860
SyntenyMoc06g04860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGCTGTCTTTTCAGAGCACCTCTCCGAATAGAATTCTTCAAAACTTTATACGCTATCCCAAATCCAAAATTGTCCGTTCTTCTCCGCTGAAGTTCCCAAATCGCTCTGCAACTACTGTTCCAATGGCGGATCTTTCCTCCGACGCTCCTTCCAGAGGATCCATCACTCATGTTATCTTCGATATGGACGGCCTCTTATTGGGTAAATTCGCTTTTCATATTCCTTCCCTTTCTCCTTCCTCATTGTTATGTTGTCATTTGCTTTCCTATCAATTCTCCAATTTCCGGATTTATCCCCCGGATCTGCTGCTCTTTTTATCATTATTATTATTGCATATTGTTCACTGCTGCTTGTAAGCAACTTACTGTTGCTTGATTCGCGCTGGTTTCTAAAATATATATCCTGCTTTGTACAAGCTAAAGTATGAGATTAGGTCACCATTGCATTGACTGTGCCTGAATCTTTGGTTGGCTATAGCGTGTACTTGTTTTCAGTACAATCTACTATTCAGCAGTTGGGAGATTTTGGATCTAAAGATAATATCTGTTAAAATTTTCATGAACTAATATGTAATTCTTCTACTGCAGATACTGAGGTGTTTTACACTAAAGTACAAGAGAACATTTTGGCAAGATTTAATAAAACTTTTGATTGGTCACTTAAGGCAAAGATGATGGGTATGAAAGCTATAGAAGCTGCTCGAGTCTTTGTTGAAGAGAGTGGAATTAGTGATTCCCTTAGTGCGGAGGACTTTCTTGTGGAAAGAGAGGACATGCTACGAAGTCTATTTCCGAATAGTGAGCTTATGCCAGGTTAGTATTACCACTTATGAGAATGCTTGATGCTTATGATCATTTTCTGTAATTAAGTCATATTTCATTGCATTCACGTGAATTTTGAGAGAGATGTCAAGTCAAACATCCACAATGTGAACGAAAATGATAACTTTACAAGGATTCAAAATTATTCTATCAATTCTGCCTGCATTTGAAAAATGATACACAGATACTGGTCACTAGTACCTTTTGTTGGAAGGCAAAATTTCATGGTGAACAGTTAATTGAAATCACTTAAGAGATGTCGACTCAGTGCATGTCAATTATGTTGGCTGTCTTCTACTTCCAACAAGCCCCCTGGTAGTGAAAGGCTGACATGGAGGTACCGAACCACCGTCCCAACGTTTCATACATAAAAGGAAAAAACTGAGAAGTAAGAAAGAAAGGACGGCATTTGATTGAGTTGAGTGTTTTTAAGGAAAAGAAATGAAACTATAAGAGTAGAGAGTTCGATATTTAAAAAGAAAGACAATGATTGGAGATATCCACATGGGGACCAGCAAATCTAGTCAATTTTGCGAACATGTAAACACTCTCCAGTCCCTATCAAAAGTTTAATACAGCACAGCATCTTGTACTTTTTAGGTGAGACTAGGGACCAATTAACCTACAGTGCCACGAGACAGACAAACTTCAAAGTTTTATCTCAGATTCGCTCACCTGTTCTGAAGTGTCTGTGTTCTCTTTCTATGCAATAGCTAGTGGTTGATGTTTAAATACGTTTTTTTAATTGTCATAAATTGCTTTTAGTTACACTCCATTTTGCCTATTTATTGCAGGAGCTAGCCGGTTGATCAAACATCTTCATGCAAAAGGAGTACCATTCGGCTTGGCAACTGGGTATGTATAGCATTCTATATGATAGCTCATAGCTTTGGCCTAGTTTTTCATTGTAAATTCCAGCCATTCTTATTAAAATCATAGTTCTTTTTTATCTCTTTTATGATTGTGTTTGTATCGCTATAAAATAAATAATTTATCTACCATTATTTTATCTGAAAATTTTCAACAGATCTCATAGACGTCATTTTGAATTAAAAACACAAAGACATGGTGAACTTTTTAAGTTGATGCATCACATTGTTCTTGGTGATGATCCAGAGGTTAAGAAGGGAAAGCCATCACCGGATGTATTTCTTGCAGCTGCTAAAAGATTTGAGGTATAGTTGTAACTTTTTAATTAAAATCTTGGTTCACTCTGTCGTTTATTTGCATATAAGTGCTTCCCCTTTTTGTGCTACAATGTGATGGTGGCTATGCCACATCTTTTAAACAGGATGCTCCCGTAGATCCCAGCAGAACCCTTGTGTTTGAAGATGCTCCATCAGGTGTTCTTGCTGCTAAAAGTGCGGGAATGTAAGTTTGGGAATTTAAATTTCTTACGTATTATAGCTCTTTACCATTTTAGTGTTTTGACCAAATGTGTTTCAATTCAACATGGAAGGGTGTGTTGCCTTTATTACCATTGTTGAGTCTCAACTACCAACAGCAGTAAATTTGCCACCCAATTTGATTAACTTTGCATCAATAGCCTCTGCTACATCTATCTTTTACAATTGATTGAAATTTTAATCTCTGGCACTCCTCAGCCACAGTTTTTCAATATAAGTCTGGCTGTGAAAGATTTGATTTTGACCAGCATATCATAATTCATAATTTTGCCAATGTTTTTGACTTTGCATATTTTGTATTTTCGATGGAGTTCCCTAGCTAGTCCAAAACTCAAATATTTGCCTGTTCATGATTTCAATGTCTAAACTCAAATATACAGTTTCTTGCCCTTATTAGTTTTGGACCTCATTATAGGTAGACTAGGAAACCAAATATATTCTATGATTGATTAAATTATAATTTTAGGCCATGTGCTTCTTTACTCGTAATATTTGTCTCTATATTCATTTTAGTTGTATTTTCGTAAAGGTGACAATTTAGTCTAAACTGTAACTAGAAGCTGACACAGAATCACATAGAGCTTTACATAAGCATCTATGTAATGGCATGTTGGCTTTTATTTAGAATTTATATCAGAGACCAAATCAATATGTTTACAGAAGTACAAAGAACAAAAAAATGAAACAAATATCGAAACGCAATTTAACTGTAATAATTTTGGTTATTGTTATTGTCACTGAAAACTTGTTTTGCCATCGGAAAGCAGGAGAGTAATTATGGTTCCAGATCCAAGGTTGGATGATTCCTATCGTAAAGATGCAGACCAAGTGTTGAGTTCCCTCTTGGATTTCAACCCCAGAGAATGGGGTCTGCCTCCTTTTGAAGATTCAGAAAGCTGA

mRNA sequence

ATGATGCTGTCTTTTCAGAGCACCTCTCCGAATAGAATTCTTCAAAACTTTATACGCTATCCCAAATCCAAAATTGTCCGTTCTTCTCCGCTGAAGTTCCCAAATCGCTCTGCAACTACTGTTCCAATGGCGGATCTTTCCTCCGACGCTCCTTCCAGAGGATCCATCACTCATGTTATCTTCGATATGGACGGCCTCTTATTGGATACTGAGGTGTTTTACACTAAAGTACAAGAGAACATTTTGGCAAGATTTAATAAAACTTTTGATTGGTCACTTAAGGCAAAGATGATGGGTATGAAAGCTATAGAAGCTGCTCGAGTCTTTGTTGAAGAGAGTGGAATTAGTGATTCCCTTAGTGCGGAGGACTTTCTTGTGGAAAGAGAGGACATGCTACGAAGTCTATTTCCGAATAGTGAGCTTATGCCAGGAGCTAGCCGGTTGATCAAACATCTTCATGCAAAAGGAGTACCATTCGGCTTGGCAACTGGATCTCATAGACGTCATTTTGAATTAAAAACACAAAGACATGGTGAACTTTTTAAGTTGATGCATCACATTGTTCTTGGTGATGATCCAGAGGTTAAGAAGGGAAAGCCATCACCGGATGTATTTCTTGCAGCTGCTAAAAGATTTGAGGATGCTCCCGTAGATCCCAGCAGAACCCTTGTGTTTGAAGATGCTCCATCAGGTGTTCTTGCTGCTAAAAGTGCGGGAATGAGAGTAATTATGGTTCCAGATCCAAGGTTGGATGATTCCTATCGTAAAGATGCAGACCAAGTGTTGAGTTCCCTCTTGGATTTCAACCCCAGAGAATGGGGTCTGCCTCCTTTTGAAGATTCAGAAAGCTGA

Coding sequence (CDS)

ATGATGCTGTCTTTTCAGAGCACCTCTCCGAATAGAATTCTTCAAAACTTTATACGCTATCCCAAATCCAAAATTGTCCGTTCTTCTCCGCTGAAGTTCCCAAATCGCTCTGCAACTACTGTTCCAATGGCGGATCTTTCCTCCGACGCTCCTTCCAGAGGATCCATCACTCATGTTATCTTCGATATGGACGGCCTCTTATTGGATACTGAGGTGTTTTACACTAAAGTACAAGAGAACATTTTGGCAAGATTTAATAAAACTTTTGATTGGTCACTTAAGGCAAAGATGATGGGTATGAAAGCTATAGAAGCTGCTCGAGTCTTTGTTGAAGAGAGTGGAATTAGTGATTCCCTTAGTGCGGAGGACTTTCTTGTGGAAAGAGAGGACATGCTACGAAGTCTATTTCCGAATAGTGAGCTTATGCCAGGAGCTAGCCGGTTGATCAAACATCTTCATGCAAAAGGAGTACCATTCGGCTTGGCAACTGGATCTCATAGACGTCATTTTGAATTAAAAACACAAAGACATGGTGAACTTTTTAAGTTGATGCATCACATTGTTCTTGGTGATGATCCAGAGGTTAAGAAGGGAAAGCCATCACCGGATGTATTTCTTGCAGCTGCTAAAAGATTTGAGGATGCTCCCGTAGATCCCAGCAGAACCCTTGTGTTTGAAGATGCTCCATCAGGTGTTCTTGCTGCTAAAAGTGCGGGAATGAGAGTAATTATGGTTCCAGATCCAAGGTTGGATGATTCCTATCGTAAAGATGCAGACCAAGTGTTGAGTTCCCTCTTGGATTTCAACCCCAGAGAATGGGGTCTGCCTCCTTTTGAAGATTCAGAAAGCTGA

Protein sequence

MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSAEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGMRVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES
Homology
BLAST of Moc06g04860 vs. NCBI nr
Match: XP_022135581.1 ((DL)-glycerol-3-phosphatase 2 [Momordica charantia])

HSP 1 Score: 561.6 bits (1446), Expect = 3.8e-156
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60
           MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI
Sbjct: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60

Query: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120
           FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS
Sbjct: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120

Query: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180
           AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL
Sbjct: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180

Query: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240
           FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM
Sbjct: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240

Query: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES
Sbjct: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 283

BLAST of Moc06g04860 vs. NCBI nr
Match: XP_008453605.1 (PREDICTED: (DL)-glycerol-3-phosphatase 2 [Cucumis melo])

HSP 1 Score: 479.6 bits (1233), Expect = 1.9e-131
Identity = 239/281 (85.05%), Postives = 257/281 (91.46%), Query Frame = 0

Query: 3   LSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVIFD 62
           L   S++P R+ QNFIR+PKSKI  SS  +F NR ATTV MA+LS D  S+GSITHVIFD
Sbjct: 5   LQSPSSTPTRLFQNFIRFPKSKIFHSSQFQFSNRPATTVSMANLSPDTTSKGSITHVIFD 64

Query: 63  MDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSAE 122
           MDGLLLDTE FYT+VQE ILAR+NKTFDWSLKAKMMG KAIEAARVFVEESGISDSLS E
Sbjct: 65  MDGLLLDTEGFYTEVQERILARYNKTFDWSLKAKMMGRKAIEAARVFVEESGISDSLSPE 124

Query: 123 DFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGELFK 182
           DFLVEREDMLRSLFP SELMPGASRLI+HLHAKGVPFGLATGSHRRHFELKTQRHGELFK
Sbjct: 125 DFLVEREDMLRSLFPQSELMPGASRLIRHLHAKGVPFGLATGSHRRHFELKTQRHGELFK 184

Query: 183 LMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGMRV 242
           LMHHIVLGDDPEVK+GKPSPD+FLAAAKRFEDAPVDP RTLVFEDAPSGVLAAK+AGM+V
Sbjct: 185 LMHHIVLGDDPEVKQGKPSPDIFLAAAKRFEDAPVDPERTLVFEDAPSGVLAAKNAGMKV 244

Query: 243 IMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           IMVPDPRLD S+  +A+QVLSSLLDFNP+EWGLPPFEDSES
Sbjct: 245 IMVPDPRLDSSHHGNANQVLSSLLDFNPKEWGLPPFEDSES 285

BLAST of Moc06g04860 vs. NCBI nr
Match: XP_023529419.1 ((DL)-glycerol-3-phosphatase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.8 bits (1231), Expect = 3.3e-131
Identity = 235/283 (83.04%), Postives = 260/283 (91.87%), Query Frame = 0

Query: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60
           M+   QS SPNR+L+NF+R  +SK ++SS   FPNR+ATTV MA+LSSD PSRGSITH+I
Sbjct: 1   MLQLLQSPSPNRLLRNFLRNSRSKPLQSSSFHFPNRTATTVSMANLSSDTPSRGSITHII 60

Query: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120
           FDMDGLLLDTE FYT+VQ+ ILAR++KTFDWSLKAKMMGMKAIEAARVFV E+GISDSLS
Sbjct: 61  FDMDGLLLDTEGFYTEVQQKILARYDKTFDWSLKAKMMGMKAIEAARVFVNETGISDSLS 120

Query: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180
           AEDFLVEREDMLRS+FPN+E MPGASRLI+HLHAKGVPFGLATGSH+RHFELKTQRHGEL
Sbjct: 121 AEDFLVEREDMLRSMFPNTEAMPGASRLIRHLHAKGVPFGLATGSHKRHFELKTQRHGEL 180

Query: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240
           FKLMHH+VLGDDPEVKKGKPSPD+FLAAAKRFE+ PVDP R LVFEDAPSGVLAAK+AGM
Sbjct: 181 FKLMHHVVLGDDPEVKKGKPSPDIFLAAAKRFEENPVDPQRILVFEDAPSGVLAAKNAGM 240

Query: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
            VIMVPDPRLD SY  DADQVLSSL+DFNP+EWGLPPFEDSES
Sbjct: 241 NVIMVPDPRLDSSYLGDADQVLSSLMDFNPKEWGLPPFEDSES 283

BLAST of Moc06g04860 vs. NCBI nr
Match: KAG7021724.1 ((DL)-glycerol-3-phosphatase 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 476.5 bits (1225), Expect = 1.6e-130
Identity = 234/283 (82.69%), Postives = 259/283 (91.52%), Query Frame = 0

Query: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60
           M+   QS SPNR+L+N +R  +SK ++SS   FPNR+ATTV MA+LSSDAPSRGSITH+I
Sbjct: 1   MLQLLQSPSPNRLLRNLLRNSRSKPLQSSSFHFPNRTATTVSMANLSSDAPSRGSITHII 60

Query: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120
           FDMDGLLLDTE FYT+VQ+ ILAR++KTFDWSLKAKMMGMKAIEAARVFV E+GISDSLS
Sbjct: 61  FDMDGLLLDTEGFYTEVQQKILARYDKTFDWSLKAKMMGMKAIEAARVFVNETGISDSLS 120

Query: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180
           AEDFL EREDMLRS+FPN+E MPGASRLI+HLHAKGVPFGLATGSH+RHFELKTQRHGEL
Sbjct: 121 AEDFLAEREDMLRSMFPNTEAMPGASRLIRHLHAKGVPFGLATGSHKRHFELKTQRHGEL 180

Query: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240
           FKLMHH+VLGDDPEVKKGKPSPD+FLAAAKRFE+ PVDP R LVFEDAPSGVLAAK+AGM
Sbjct: 181 FKLMHHVVLGDDPEVKKGKPSPDIFLAAAKRFEEHPVDPQRILVFEDAPSGVLAAKNAGM 240

Query: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
            VIMVPDPRLD SY  DADQVLSSL+DFNP+EWGLPPFEDSES
Sbjct: 241 NVIMVPDPRLDSSYLGDADQVLSSLMDFNPKEWGLPPFEDSES 283

BLAST of Moc06g04860 vs. NCBI nr
Match: XP_038878592.1 ((DL)-glycerol-3-phosphatase 2 [Benincasa hispida])

HSP 1 Score: 471.9 bits (1213), Expect = 4.0e-129
Identity = 234/282 (82.98%), Postives = 256/282 (90.78%), Query Frame = 0

Query: 2   MLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVIF 61
           +L   S +  R+ QNF R+P SKI+  S L+FPN +ATTV MA+LS D+ SRGSITH+IF
Sbjct: 4   LLQIPSPTSTRLFQNFRRFPNSKILNFSQLQFPNPTATTVSMANLSPDSTSRGSITHIIF 63

Query: 62  DMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSA 121
           DMDGLLLDTE FYT+VQE ILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSA
Sbjct: 64  DMDGLLLDTEGFYTEVQEKILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSA 123

Query: 122 EDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGELF 181
           EDFLVEREDMLRSLFP SELMPGASRLI+HLHAKGVPFGLATGSHRRHFELKTQRHGE+F
Sbjct: 124 EDFLVEREDMLRSLFPQSELMPGASRLIRHLHAKGVPFGLATGSHRRHFELKTQRHGEIF 183

Query: 182 KLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGMR 241
           KLMHH VLGDDPEVK+GKPSPD+FLAAAKRFEDAPV P + LVFEDAPSGVLAAK+AGM+
Sbjct: 184 KLMHHTVLGDDPEVKQGKPSPDIFLAAAKRFEDAPVYPEKILVFEDAPSGVLAAKNAGMK 243

Query: 242 VIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           V+MVPDPRLD SY  +A+QVLSSLLDFNP+EWGLPPFEDSES
Sbjct: 244 VVMVPDPRLDSSYHGNANQVLSSLLDFNPKEWGLPPFEDSES 285

BLAST of Moc06g04860 vs. ExPASy Swiss-Prot
Match: Q8VZP1 ((DL)-glycerol-3-phosphatase 2 OS=Arabidopsis thaliana OX=3702 GN=GPP2 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 8.7e-103
Identity = 182/239 (76.15%), Postives = 208/239 (87.03%), Query Frame = 0

Query: 43  MADLSSDAPSRGSITHVIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKA 102
           M++ ++    RGSITHVIFDMDGLLLDTE FYT+VQE ILARFNK FDWSLKAKMMG KA
Sbjct: 1   MSNPAAVTAGRGSITHVIFDMDGLLLDTEKFYTEVQEIILARFNKKFDWSLKAKMMGRKA 60

Query: 103 IEAARVFVEESGISDSLSAEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLA 162
           IEAAR+FVEESGISDSLSAEDFLVERE ML+ LFP SELMPGASRLIKHLH K +P  +A
Sbjct: 61  IEAARIFVEESGISDSLSAEDFLVERESMLQDLFPTSELMPGASRLIKHLHVKNIPICIA 120

Query: 163 TGSHRRHFELKTQRHGELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRT 222
           TG+H RH++LKTQRH ELF LMHH+V GDDPEVK+GKP+PD FLAAA+RF+D PVD  + 
Sbjct: 121 TGTHTRHYDLKTQRHRELFSLMHHVVRGDDPEVKQGKPAPDGFLAAARRFKDGPVDSQKV 180

Query: 223 LVFEDAPSGVLAAKSAGMRVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDS 282
           LVFEDAPSGVLAAK+AGM V+MVPDPRLD S++  ADQ+++SL+DF P EWGLPPFEDS
Sbjct: 181 LVFEDAPSGVLAAKNAGMNVVMVPDPRLDISHQDVADQIITSLVDFKPEEWGLPPFEDS 239

BLAST of Moc06g04860 vs. ExPASy Swiss-Prot
Match: F4JTE7 ((DL)-glycerol-3-phosphatase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GPP1 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.5e-102
Identity = 189/274 (68.98%), Postives = 218/274 (79.56%), Query Frame = 0

Query: 26  VRSSPLKFPNRSATTVP-----MADLSSDAPS-----------RGSITHVIFDMDGLLLD 85
           +  SP  FP +    VP     +A +S+ A +           RGSITHVIFDMDGLLLD
Sbjct: 25  IAPSPKVFPRKPVIRVPASLRFVATMSTPAAAVNATVTVTDAGRGSITHVIFDMDGLLLD 84

Query: 86  TEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSAEDFLVERE 145
           TE FYT+VQE ILAR+NKTFDWSLKAKMMG KAIEAAR+FV+ESGISDSLSAEDF+VERE
Sbjct: 85  TEKFYTEVQEKILARYNKTFDWSLKAKMMGRKAIEAARLFVDESGISDSLSAEDFIVERE 144

Query: 146 DMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGELFKLMHHIVL 205
            ML+ LFP S+LMPGASRL++HLH KG+P  +ATG+H RHF+LKTQRH ELF LMHH+V 
Sbjct: 145 SMLQDLFPTSDLMPGASRLLRHLHGKGIPICIATGTHTRHFDLKTQRHRELFSLMHHVVR 204

Query: 206 GDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGMRVIMVPDPR 265
           GDDPEVK+GKP+PD FLAA++RFED PVDP + LVFEDAPSGV AAK+AGM VIMVPD R
Sbjct: 205 GDDPEVKEGKPAPDGFLAASRRFEDGPVDPRKVLVFEDAPSGVQAAKNAGMNVIMVPDSR 264

Query: 266 LDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           LD SY   ADQVL+SLLDF P EWGLP F+DS +
Sbjct: 265 LDKSYCNVADQVLASLLDFKPEEWGLPSFQDSHN 298

BLAST of Moc06g04860 vs. ExPASy Swiss-Prot
Match: Q08623 (Pseudouridine-5'-phosphatase OS=Homo sapiens OX=9606 GN=PUDP PE=1 SV=3)

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-56
Identity = 113/224 (50.45%), Postives = 150/224 (66.96%), Query Frame = 0

Query: 56  ITHVIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGI 115
           +TH+IFDMDGLLLDTE  Y+ V + I  R++K + W +K+ +MG KA+EAA++ ++   +
Sbjct: 8   VTHLIFDMDGLLLDTERLYSVVFQEICNRYDKKYSWDVKSLVMGKKALEAAQIIIDV--L 67

Query: 116 SDSLSAEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQ 175
              +S E+ + E +  L+ +FP + LMPGA +LI HL   G+PF LAT S    F++KT 
Sbjct: 68  QLPMSKEELVEESQTKLKEVFPTAALMPGAEKLIIHLRKHGIPFALATSSGSASFDMKTS 127

Query: 176 RHGELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAA 235
           RH E F L  HIVLGDDPEV+ GKP PD+FLA AKRF   P    + LVFEDAP+GV AA
Sbjct: 128 RHKEFFSLFSHIVLGDDPEVQHGKPDPDIFLACAKRFSPPPA-MEKCLVFEDAPNGVEAA 187

Query: 236 KSAGMRVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFE 280
            +AGM+V+MVPD  L       A  VL+SL DF P  +GLP +E
Sbjct: 188 LAAGMQVVMVPDGNLSRDLTTKATLVLNSLQDFQPELFGLPSYE 228

BLAST of Moc06g04860 vs. ExPASy Swiss-Prot
Match: Q94529 (Probable pseudouridine-5'-phosphatase OS=Drosophila melanogaster OX=7227 GN=Gs1l PE=2 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 1.4e-52
Identity = 114/226 (50.44%), Postives = 145/226 (64.16%), Query Frame = 0

Query: 56  ITHVIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGI 115
           +TH +FDMDGLLLDTE  YT   E IL  + KT+ + +K ++MG++    AR  VE   +
Sbjct: 9   VTHCVFDMDGLLLDTERLYTVATEMILEPYGKTYPFEIKEQVMGLQTEPLARFMVEHYEL 68

Query: 116 SDSLSAEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQ 175
              +S E++  ++      L  N++LMPGA RL++HLHA  VPF LAT S     ELKT 
Sbjct: 69  --PMSWEEYARQQRANTEILMRNAQLMPGAERLLRHLHANKVPFCLATSSGADMVELKTA 128

Query: 176 RHGELFKLMHHIVLG-DDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLA 235
           +H ELF L +H V G  D EV  GKP+PD+FL AA RF   P  PS  LVFED+P+GV A
Sbjct: 129 QHRELFSLFNHKVCGSSDKEVVNGKPAPDIFLVAAGRF-GVPPKPSDCLVFEDSPNGVTA 188

Query: 236 AKSAGMRVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFED 281
           A SAGM+V+MVPDPRL       A QVL+SL DF P ++GLP F D
Sbjct: 189 ANSAGMQVVMVPDPRLSQEKTSHATQVLASLADFKPEQFGLPAFTD 231

BLAST of Moc06g04860 vs. ExPASy Swiss-Prot
Match: Q9D5U5 (Pseudouridine-5'-phosphatase OS=Mus musculus OX=10090 GN=Pudp PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.3e-50
Identity = 109/230 (47.39%), Postives = 140/230 (60.87%), Query Frame = 0

Query: 51  PSRGSITHVIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFV 110
           P    +T +IFD+DGL+L+TE  YT V E I  R+ K ++W +K+ +MG KA+E A+  V
Sbjct: 8   PQFRPVTPLIFDLDGLILNTEDLYTDVFEEICNRYGKKYNWDVKSLVMGKKALETAQTIV 67

Query: 111 EESGISDSLSAEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHF 170
           E   +   +S E+ L E ++ L+ +   +  MPGA  LI HL    +PF LAT S    F
Sbjct: 68  EFLNL--PISKEELLKESQEKLQMVLHTAGFMPGAEELIHHLKKHRLPFALATSSETVTF 127

Query: 171 ELKTQRHGELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPS 230
           + KT RH   F L HHIVLGDDPEVK GKP  D+FL  AKRF   P DP   LVFED+P+
Sbjct: 128 QTKTSRHTGFFGLFHHIVLGDDPEVKNGKPGMDIFLTCAKRFSPPP-DPKDCLVFEDSPN 187

Query: 231 GVLAAKSAGMRVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFED 281
           GV AA   GM+V+MVP   L     + A  VLSSL DF P  +GLP F +
Sbjct: 188 GVEAAIHCGMQVVMVPHENLSADLTRKATLVLSSLHDFKPELFGLPAFTE 234

BLAST of Moc06g04860 vs. ExPASy TrEMBL
Match: A0A6J1C159 ((DL)-glycerol-3-phosphatase 2 OS=Momordica charantia OX=3673 GN=LOC111007496 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.9e-156
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60
           MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI
Sbjct: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60

Query: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120
           FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS
Sbjct: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120

Query: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180
           AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL
Sbjct: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180

Query: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240
           FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM
Sbjct: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240

Query: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES
Sbjct: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 283

BLAST of Moc06g04860 vs. ExPASy TrEMBL
Match: A0A1S3BXU4 ((DL)-glycerol-3-phosphatase 2 OS=Cucumis melo OX=3656 GN=LOC103494264 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 9.3e-132
Identity = 239/281 (85.05%), Postives = 257/281 (91.46%), Query Frame = 0

Query: 3   LSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVIFD 62
           L   S++P R+ QNFIR+PKSKI  SS  +F NR ATTV MA+LS D  S+GSITHVIFD
Sbjct: 5   LQSPSSTPTRLFQNFIRFPKSKIFHSSQFQFSNRPATTVSMANLSPDTTSKGSITHVIFD 64

Query: 63  MDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSAE 122
           MDGLLLDTE FYT+VQE ILAR+NKTFDWSLKAKMMG KAIEAARVFVEESGISDSLS E
Sbjct: 65  MDGLLLDTEGFYTEVQERILARYNKTFDWSLKAKMMGRKAIEAARVFVEESGISDSLSPE 124

Query: 123 DFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGELFK 182
           DFLVEREDMLRSLFP SELMPGASRLI+HLHAKGVPFGLATGSHRRHFELKTQRHGELFK
Sbjct: 125 DFLVEREDMLRSLFPQSELMPGASRLIRHLHAKGVPFGLATGSHRRHFELKTQRHGELFK 184

Query: 183 LMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGMRV 242
           LMHHIVLGDDPEVK+GKPSPD+FLAAAKRFEDAPVDP RTLVFEDAPSGVLAAK+AGM+V
Sbjct: 185 LMHHIVLGDDPEVKQGKPSPDIFLAAAKRFEDAPVDPERTLVFEDAPSGVLAAKNAGMKV 244

Query: 243 IMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           IMVPDPRLD S+  +A+QVLSSLLDFNP+EWGLPPFEDSES
Sbjct: 245 IMVPDPRLDSSHHGNANQVLSSLLDFNPKEWGLPPFEDSES 285

BLAST of Moc06g04860 vs. ExPASy TrEMBL
Match: A0A6J1F0H8 ((DL)-glycerol-3-phosphatase 2 OS=Cucurbita moschata OX=3662 GN=LOC111441220 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 7.3e-129
Identity = 232/283 (81.98%), Postives = 257/283 (90.81%), Query Frame = 0

Query: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60
           M+   QS SPNR+L+N +   +SK ++SS   F NR+ATTV MA+LSSD PSRGSITH+I
Sbjct: 1   MLQLLQSPSPNRLLRNLLPNSRSKPLQSSSFHFLNRTATTVSMANLSSDTPSRGSITHII 60

Query: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120
           FDMDGLLLDTE FYT+VQ+ ILAR++KTFDWSLKAKMMGMKAIEAARVFV E+GISDSLS
Sbjct: 61  FDMDGLLLDTEGFYTEVQQKILARYDKTFDWSLKAKMMGMKAIEAARVFVNETGISDSLS 120

Query: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180
           AEDFLVEREDMLRS+FPN+E MPGASRLI+HLHAKGVPFGLATGSH+RHFELKTQRHGEL
Sbjct: 121 AEDFLVEREDMLRSMFPNTEAMPGASRLIRHLHAKGVPFGLATGSHKRHFELKTQRHGEL 180

Query: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240
           FKLMHH+VLGDDPEVKKGKPSPD+FLAAAKRFE+ PVDP R LVFEDAPSGVLAAK+AGM
Sbjct: 181 FKLMHHVVLGDDPEVKKGKPSPDIFLAAAKRFEEHPVDPQRILVFEDAPSGVLAAKNAGM 240

Query: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
            VIMVPDPRLD SY  DADQVLSSL+DFNP+EWGLPPFEDSES
Sbjct: 241 NVIMVPDPRLDSSYLGDADQVLSSLMDFNPKEWGLPPFEDSES 283

BLAST of Moc06g04860 vs. ExPASy TrEMBL
Match: A0A0A0LZT1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423140 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 8.1e-128
Identity = 233/281 (82.92%), Postives = 252/281 (89.68%), Query Frame = 0

Query: 3   LSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVIFD 62
           L   S++P R+ Q+FIR+P S I RSS  +FPNR+  TV MA LS D  S GSITHVIFD
Sbjct: 5   LQSPSSTPTRLFQHFIRFPNSNIFRSSQFQFPNRTPITVSMATLSPDTSSEGSITHVIFD 64

Query: 63  MDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSAE 122
           MDGLLLDTE FYT+VQE ILAR++KTFDWSLKAKMMG KAIEAARVFVEESGISDSLS E
Sbjct: 65  MDGLLLDTEGFYTEVQEKILARYDKTFDWSLKAKMMGRKAIEAARVFVEESGISDSLSPE 124

Query: 123 DFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGELFK 182
           DFLVEREDMLRSLFP SELMPGASRLI+HLHAKGVPFGLATGSHRRHFELKTQRHGELFK
Sbjct: 125 DFLVEREDMLRSLFPQSELMPGASRLIRHLHAKGVPFGLATGSHRRHFELKTQRHGELFK 184

Query: 183 LMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGMRV 242
           LMHHIVLGDDPEVK+GKPSPD+FLAAAKRF DAPVD  RTLVFEDAPSGVLAAK+AGM+V
Sbjct: 185 LMHHIVLGDDPEVKQGKPSPDIFLAAAKRFADAPVDAERTLVFEDAPSGVLAAKNAGMKV 244

Query: 243 IMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           IMVPDPRLD S+  +A+QVLSSLLDFNP+EWGLPPFEDSES
Sbjct: 245 IMVPDPRLDSSHHGNANQVLSSLLDFNPKEWGLPPFEDSES 285

BLAST of Moc06g04860 vs. ExPASy TrEMBL
Match: A0A6J1KTW1 ((DL)-glycerol-3-phosphatase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497173 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.5e-126
Identity = 233/283 (82.33%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 1   MMLSFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSSDAPSRGSITHVI 60
           M+   QS SPNR+L+NF+   +SK     PL   NR+ATTV MA+LSSDAPSRGSITH+I
Sbjct: 1   MLQLLQSPSPNRLLRNFLLNSRSK-----PLH--NRTATTVSMANLSSDAPSRGSITHII 60

Query: 61  FDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLS 120
           FDMDGLLLDTE FYT+VQ+ ILAR++KTFDWSLKAKMMGMKAIEAARVFV E+GISDSLS
Sbjct: 61  FDMDGLLLDTEGFYTEVQQKILARYDKTFDWSLKAKMMGMKAIEAARVFVNETGISDSLS 120

Query: 121 AEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGEL 180
           AEDFLVEREDMLRS+FPN+E M GASRLI+HLHAKGVPFGLATGSH+RHFELKTQRHGEL
Sbjct: 121 AEDFLVEREDMLRSMFPNTEAMQGASRLIRHLHAKGVPFGLATGSHKRHFELKTQRHGEL 180

Query: 181 FKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGM 240
           FKLMHHIVLGDDPEVKKGKPSPD+FLAAAKRFE+ PVDP R LVFEDAPSGVLAAK+AGM
Sbjct: 181 FKLMHHIVLGDDPEVKKGKPSPDIFLAAAKRFEEHPVDPQRILVFEDAPSGVLAAKNAGM 240

Query: 241 RVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
            VIMVPDPRLD SY  DADQVLSSL+DFNP+EWGLPPFEDSES
Sbjct: 241 NVIMVPDPRLDSSYLGDADQVLSSLMDFNPKEWGLPPFEDSES 276

BLAST of Moc06g04860 vs. TAIR 10
Match: AT5G57440.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 374.8 bits (961), Expect = 6.2e-104
Identity = 182/239 (76.15%), Postives = 208/239 (87.03%), Query Frame = 0

Query: 43  MADLSSDAPSRGSITHVIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKA 102
           M++ ++    RGSITHVIFDMDGLLLDTE FYT+VQE ILARFNK FDWSLKAKMMG KA
Sbjct: 1   MSNPAAVTAGRGSITHVIFDMDGLLLDTEKFYTEVQEIILARFNKKFDWSLKAKMMGRKA 60

Query: 103 IEAARVFVEESGISDSLSAEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLA 162
           IEAAR+FVEESGISDSLSAEDFLVERE ML+ LFP SELMPGASRLIKHLH K +P  +A
Sbjct: 61  IEAARIFVEESGISDSLSAEDFLVERESMLQDLFPTSELMPGASRLIKHLHVKNIPICIA 120

Query: 163 TGSHRRHFELKTQRHGELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRT 222
           TG+H RH++LKTQRH ELF LMHH+V GDDPEVK+GKP+PD FLAAA+RF+D PVD  + 
Sbjct: 121 TGTHTRHYDLKTQRHRELFSLMHHVVRGDDPEVKQGKPAPDGFLAAARRFKDGPVDSQKV 180

Query: 223 LVFEDAPSGVLAAKSAGMRVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFEDS 282
           LVFEDAPSGVLAAK+AGM V+MVPDPRLD S++  ADQ+++SL+DF P EWGLPPFEDS
Sbjct: 181 LVFEDAPSGVLAAKNAGMNVVMVPDPRLDISHQDVADQIITSLVDFKPEEWGLPPFEDS 239

BLAST of Moc06g04860 vs. TAIR 10
Match: AT4G25840.1 (glycerol-3-phosphatase 1 )

HSP 1 Score: 374.0 bits (959), Expect = 1.1e-103
Identity = 189/274 (68.98%), Postives = 218/274 (79.56%), Query Frame = 0

Query: 26  VRSSPLKFPNRSATTVP-----MADLSSDAPS-----------RGSITHVIFDMDGLLLD 85
           +  SP  FP +    VP     +A +S+ A +           RGSITHVIFDMDGLLLD
Sbjct: 25  IAPSPKVFPRKPVIRVPASLRFVATMSTPAAAVNATVTVTDAGRGSITHVIFDMDGLLLD 84

Query: 86  TEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDSLSAEDFLVERE 145
           TE FYT+VQE ILAR+NKTFDWSLKAKMMG KAIEAAR+FV+ESGISDSLSAEDF+VERE
Sbjct: 85  TEKFYTEVQEKILARYNKTFDWSLKAKMMGRKAIEAARLFVDESGISDSLSAEDFIVERE 144

Query: 146 DMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHGELFKLMHHIVL 205
            ML+ LFP S+LMPGASRL++HLH KG+P  +ATG+H RHF+LKTQRH ELF LMHH+V 
Sbjct: 145 SMLQDLFPTSDLMPGASRLLRHLHGKGIPICIATGTHTRHFDLKTQRHRELFSLMHHVVR 204

Query: 206 GDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSAGMRVIMVPDPR 265
           GDDPEVK+GKP+PD FLAA++RFED PVDP + LVFEDAPSGV AAK+AGM VIMVPD R
Sbjct: 205 GDDPEVKEGKPAPDGFLAASRRFEDGPVDPRKVLVFEDAPSGVQAAKNAGMNVIMVPDSR 264

Query: 266 LDDSYRKDADQVLSSLLDFNPREWGLPPFEDSES 284
           LD SY   ADQVL+SLLDF P EWGLP F+DS +
Sbjct: 265 LDKSYCNVADQVLASLLDFKPEEWGLPSFQDSHN 298

BLAST of Moc06g04860 vs. TAIR 10
Match: AT4G21470.1 (riboflavin kinase/FMN hydrolase )

HSP 1 Score: 145.6 bits (366), Expect = 6.1e-35
Identity = 85/222 (38.29%), Postives = 125/222 (56.31%), Query Frame = 0

Query: 59  VIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKMMGMKAIEAARVFVEESGISDS 118
           V+ D+DG L++T+     +    L ++ K +D     K++G   +EAA   VE+  +   
Sbjct: 14  VLIDLDGTLINTDGVVGDILRKYLCKYGKQWDGRESLKIVGKTPVEAATTIVEDYEL--P 73

Query: 119 LSAEDFLVEREDMLRSLFPNSELMPGASRLIKHLHAKGVPFGLATGSHRRHFELKTQRHG 178
              ++F  E   +  +     + +PGA+RLI+HL   GVP  LA+ S R + E K   H 
Sbjct: 74  CKVDEFNSEFYPLFSAQMDKIKSLPGANRLIRHLKCHGVPVALASNSSRANIESKISYH- 133

Query: 179 ELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFEDAPVDPSRTLVFEDAPSGVLAAKSA 238
           E +K    +++G D EV KGKPSPD+FL AAKR +    DP+  LV ED+  GV+A K+A
Sbjct: 134 EGWKECFSVIVGSD-EVSKGKPSPDIFLEAAKRLKK---DPADCLVIEDSVPGVMAGKAA 193

Query: 239 GMRVIMVPDPRLDDSYRKDADQVLSSLLDFNPREWGLPPFED 281
           G +VI VP           AD+V++SLLD    +WGLPPF+D
Sbjct: 194 GTKVIAVPSLPKQTHLYTSADEVINSLLDIRLEKWGLPPFQD 228

BLAST of Moc06g04860 vs. TAIR 10
Match: AT1G56500.1 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 4.0e-10
Identity = 64/225 (28.44%), Postives = 96/225 (42.67%), Query Frame = 0

Query: 38  ATTVPMADLSSDAPSRGSITHVIFDMDGLLLDTEVFYTKVQENILARFNKTFDWSLKAKM 97
           A   P A +++D    G ++ V+FDMDG+L ++E    +   ++                
Sbjct: 58  AAESPAATIATD--DWGKVSAVLFDMDGVLCNSEDLSRRAAVDVFTEMGVEVTVDDFVPF 117

Query: 98  MG---MKAIEAARVFVEESGISDSLSAEDFLVEREDMLRSLFPNSEL-MPGASRLIKHLH 157
           MG    K +       E  G     + E F     D      P S +  PGA  L+    
Sbjct: 118 MGTGEAKFLGGVASVKEVKGFDPDAAKERFFEIYLDKYAK--PESGIGFPGALELVTECK 177

Query: 158 AKGVPFGLATGSHRRHFELKTQRHGELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFE 217
            KG+   +A+ + R   +   +  G    +   IV  D    +  KP+PD+FLAAAK   
Sbjct: 178 NKGLKVAVASSADRIKVDANLKAAGLSLTMFDAIVSAD--AFENLKPAPDIFLAAAKIL- 237

Query: 218 DAPVDPSRTLVFEDAPSGVLAAKSAGMRVIMVPDPRLDDSYRKDA 259
              V  S  +V EDA +GV AA++A MR I V    L ++  KDA
Sbjct: 238 --GVPTSECVVIEDALAGVQAAQAANMRCIAV-KTTLSEAILKDA 272

BLAST of Moc06g04860 vs. TAIR 10
Match: AT3G48420.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-08
Identity = 76/293 (25.94%), Postives = 123/293 (41.98%), Query Frame = 0

Query: 4   SFQSTSPNRILQNFIRYPKSKIVRSSPLKFPNRSATTVPMADLSS-----DAPSRGSIT- 63
           S  S+  +++  +FI  P +   ++  L+F  +S    PM   SS        +  S+T 
Sbjct: 17  SSSSSIQSKLSPSFI--PNAAPAKAVKLRFNGKSLRAKPMVYRSSRSVGVTCSASSSLTT 76

Query: 64  ---HVIFDMDGLLLDTEVFYTKVQENILAR---FNKTFDWSLKAKMM----GMKAIEA-- 123
               ++FD DG+L+DTE    ++  N   +    N T+D  L  +++    G + + A  
Sbjct: 77  LPSALLFDCDGVLVDTEKDGHRISFNDTFKERDLNVTWDVDLYGELLKIGGGKERMTAYF 136

Query: 124 ARVFVEESGISDSLSAEDFLVEREDMLRSLF------PNSELMPGASRLIKHLHAKGVPF 183
            +V   E    D    ++F+         LF          L PG ++L+      GV  
Sbjct: 137 NKVGWPEKAPKDEAERKEFIAGLHKQKTELFMVLIEKKLLPLRPGVAKLVDQALTNGVKV 196

Query: 184 GLATGSHRRHFE-----LKTQRHGELFKLMHHIVLGDDPEVKKGKPSPDVFLAAAKRFED 243
            + + S+ +        L      E  K    I  GD   V K KP P ++  AA   E 
Sbjct: 197 AVCSTSNEKAVSAIVSCLLGPERAEKIK----IFAGD--VVPKKKPDPAIYNLAA---ET 256

Query: 244 APVDPSRTLVFEDAPSGVLAAKSAGMRVIMVPDPRLDDSYRKDADQVLSSLLD 268
             VDPS+ +V ED+  G+ AAK+AGM  I+       D   ++AD V   + D
Sbjct: 257 LGVDPSKCVVVEDSAIGLAAAKAAGMTCIVTKSGYTADEDFENADAVFDCIGD 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135581.13.8e-156100.00(DL)-glycerol-3-phosphatase 2 [Momordica charantia][more]
XP_008453605.11.9e-13185.05PREDICTED: (DL)-glycerol-3-phosphatase 2 [Cucumis melo][more]
XP_023529419.13.3e-13183.04(DL)-glycerol-3-phosphatase 2 [Cucurbita pepo subsp. pepo][more]
KAG7021724.11.6e-13082.69(DL)-glycerol-3-phosphatase 1, mitochondrial [Cucurbita argyrosperma subsp. argy... [more]
XP_038878592.14.0e-12982.98(DL)-glycerol-3-phosphatase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8VZP18.7e-10376.15(DL)-glycerol-3-phosphatase 2 OS=Arabidopsis thaliana OX=3702 GN=GPP2 PE=1 SV=1[more]
F4JTE71.5e-10268.98(DL)-glycerol-3-phosphatase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q086231.2e-5650.45Pseudouridine-5'-phosphatase OS=Homo sapiens OX=9606 GN=PUDP PE=1 SV=3[more]
Q945291.4e-5250.44Probable pseudouridine-5'-phosphatase OS=Drosophila melanogaster OX=7227 GN=Gs1l... [more]
Q9D5U51.3e-5047.39Pseudouridine-5'-phosphatase OS=Mus musculus OX=10090 GN=Pudp PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C1591.9e-156100.00(DL)-glycerol-3-phosphatase 2 OS=Momordica charantia OX=3673 GN=LOC111007496 PE=... [more]
A0A1S3BXU49.3e-13285.05(DL)-glycerol-3-phosphatase 2 OS=Cucumis melo OX=3656 GN=LOC103494264 PE=4 SV=1[more]
A0A6J1F0H87.3e-12981.98(DL)-glycerol-3-phosphatase 2 OS=Cucurbita moschata OX=3662 GN=LOC111441220 PE=4... [more]
A0A0A0LZT18.1e-12882.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423140 PE=4 SV=1[more]
A0A6J1KTW14.5e-12682.33(DL)-glycerol-3-phosphatase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
Match NameE-valueIdentityDescription
AT5G57440.16.2e-10476.15Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT4G25840.11.1e-10368.98glycerol-3-phosphatase 1 [more]
AT4G21470.16.1e-3538.29riboflavin kinase/FMN hydrolase [more]
AT1G56500.14.0e-1028.44haloacid dehalogenase-like hydrolase family protein [more]
AT3G48420.11.3e-0825.94Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006439HAD hydrolase, subfamily IATIGRFAMTIGR01509TIGR01509coord: 132..245
e-value: 1.5E-12
score: 45.9
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 59..265
e-value: 3.6E-72
score: 244.4
IPR023198Phosphoglycolate phosphatase-like, domain 2GENE3D1.10.150.240Putative phosphatase; domain 2coord: 70..139
e-value: 3.6E-72
score: 244.4
IPR041492Haloacid dehalogenase-like hydrolasePFAMPF13419HAD_2coord: 59..245
e-value: 9.1E-23
score: 81.3
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 55..268
e-value: 5.1E-40
score: 132.3
NoneNo IPR availableSFLDSFLDG01135C1.5.6:_HAD,_Beta-PGM,_Phosphcoord: 55..268
e-value: 5.1E-40
score: 132.3
NoneNo IPR availablePANTHERPTHR18901:SF41(DL)-GLYCEROL-3-PHOSPHATASE 1, MITOCHONDRIALcoord: 31..282
NoneNo IPR availablePANTHERPTHR189012-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2coord: 31..282
NoneNo IPR availableCDDcd07529HAD_AtGPP-likecoord: 56..247
e-value: 9.42341E-108
score: 308.507
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 56..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g04860.1Moc06g04860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0006114 glycerol biosynthetic process
molecular_function GO:0043136 glycerol-3-phosphatase activity