Moc06g03210 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g03210
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCalcium-binding protein CML15
Locationchr6: 2280720 .. 2281202 (+)
RNA-Seq ExpressionMoc06g03210
SyntenyMoc06g03210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCGCTCGCAGTTGAGCAGCTGAATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAGTTTGCATCTGTAATGGCTAAATCCGCATCTGAATTTCTAGGGCTTGCAATATCTTGA

mRNA sequence

ATGGCTGCGCTCGCAGTTGAGCAGCTGAATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAGTTTGCATCTGTAATGGCTAAATCCGCATCTGAATTTCTAGGGCTTGCAATATCTTGA

Coding sequence (CDS)

ATGGCTGCGCTCGCAGTTGAGCAGCTGAATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAGTTTGCATCTGTAATGGCTAAATCCGCATCTGAATTTCTAGGGCTTGCAATATCTTGA

Protein sequence

MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS
Homology
[Close]
BLAST of Moc06g03210 vs. NCBI nr
Match: XP_022135250.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 306.6 bits (784), Expect = 1.3e-79
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 160

[Close]
BLAST of Moc06g03210 vs. NCBI nr
Match: XP_004138337.2 (probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probable calcium-binding protein CML15 [Cucumis sativus])

HSP 1 Score: 302.0 bits (772), Expect = 3.1e-78
Identity = 156/160 (97.50%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSA++FLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of Moc06g03210 vs. NCBI nr
Match: KAG6589571.1 (putative calcium-binding protein CML15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 302.0 bits (772), Expect = 3.1e-78
Identity = 157/160 (98.12%), Postives = 159/160 (99.38%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGN
Sbjct: 67  MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGN 126

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 127 GSVEFDELVTAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 186

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAI+
Sbjct: 187 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIA 226

[Close]
BLAST of Moc06g03210 vs. NCBI nr
Match: KAE8652452.1 (hypothetical protein Csa_013816 [Cucumis sativus])

HSP 1 Score: 302.0 bits (772), Expect = 3.1e-78
Identity = 156/160 (97.50%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGN
Sbjct: 97  MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 156

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 157 GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 216

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSA++FLGLAIS
Sbjct: 217 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 256

[Close]
BLAST of Moc06g03210 vs. NCBI nr
Match: XP_022921807.1 (probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023516651.1 probable calcium-binding protein CML15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.0 bits (772), Expect = 3.1e-78
Identity = 157/160 (98.12%), Postives = 159/160 (99.38%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAI+
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIA 160

[Close]
BLAST of Moc06g03210 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 3.4e-64
Identity = 120/154 (77.92%), Postives = 142/154 (92.21%), Query Frame = 0 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFD 66
           +Q+ QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVL+A+MD+NGNG VEFD
Sbjct: 3   DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFD 62

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV  I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM
Sbjct: 63  ELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEM 122

Query: 127 MKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           +KEADT+GDGVISF EFAS+MAKSA ++ GL I+
Sbjct: 123 IKEADTNGDGVISFGEFASIMAKSAVDYFGLKIN 156

[Close]
BLAST of Moc06g03210 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 1.4e-62
Identity = 120/154 (77.92%), Postives = 137/154 (88.96%), Query Frame = 0 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFD 66
           +Q+ QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +L+  +D NGNGSVEFD
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEM
Sbjct: 68  ELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEM 127

Query: 127 MKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           M EAD++GDGVISFNEF+ +MAKSA++FLGL  S
Sbjct: 128 MTEADSNGDGVISFNEFSHIMAKSAADFLGLTAS 161

[Close]
BLAST of Moc06g03210 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.7e-55
Identity = 109/152 (71.71%), Postives = 131/152 (86.18%), Query Frame = 0 

Query: 8   QLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDE 67
           QL QLR++F RFDM+ DGSLT LELAALLRSLGL+P+GD++H L+A MDANGNGSVEFDE
Sbjct: 22  QLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDE 81

Query: 68  LVTAIMPDFNEEV-MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 127
           L  AI P    +  +V+Q QLLEVFR+FDRDGNG+I+AAELA SMA++GQPLT+ ELT M
Sbjct: 82  LAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRM 141

Query: 128 MKEADTDGDGVISFNEFASVMAKSASEFLGLA 159
           M++ADTDGDGVISF EFA+VMAKSA +FLG+A
Sbjct: 142 MRDADTDGDGVISFKEFAAVMAKSALDFLGVA 173

[Close]
BLAST of Moc06g03210 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 4.8e-50
Identity = 104/162 (64.20%), Postives = 129/162 (79.63%), Query Frame = 0 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKP-SGDQIHVLVANMDANGN 61
           A L  EQL QL +IF RFD+D DGSLT LELAALLRSLGL+P +GD+IH L+A +DA+GN
Sbjct: 86  ARLRGEQLRQLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGN 145

Query: 62  GSVEFDELVTA----IMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQ 121
           G+VEFDEL ++    I+      V V+Q +L E FR+FDRDGNG+I+AAELA SMA+MG 
Sbjct: 146 GTVEFDELASSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGH 205

Query: 122 PLTYRELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLA 159
           P+ Y ELT+MM+EADTDGDG+ISF EF ++MAKSA +FLGLA
Sbjct: 206 PICYAELTDMMREADTDGDGLISFEEFTAIMAKSALDFLGLA 247

[Close]
BLAST of Moc06g03210 vs. ExPASy Swiss-Prot
Match: Q9M8U1 (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 5.5e-38
Identity = 86/150 (57.33%), Postives = 99/150 (66.00%), Query Frame = 0 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNG 61
           A L  EQL +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG
Sbjct: 11  AKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNG 70

Query: 62  SVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYR 121
            VEF E V  + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  
Sbjct: 71  LVEFSEFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAE 130

Query: 122 ELTEMMKEADTDGDGVISFNEFASVMAKSA 152
           ELT M+KEAD DGDG I F EF   +  +A
Sbjct: 131 ELTGMIKEADRDGDGCIDFQEFVQAITSAA 159

[Close]
BLAST of Moc06g03210 vs. ExPASy TrEMBL
Match: A0A6J1C0M6 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111007259 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.1e-80
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 160

[Close]
BLAST of Moc06g03210 vs. ExPASy TrEMBL
Match: A0A0A0LUS7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-78
Identity = 156/160 (97.50%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSA++FLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of Moc06g03210 vs. ExPASy TrEMBL
Match: A0A6J1E1G5 (probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC111429950 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-78
Identity = 157/160 (98.12%), Postives = 159/160 (99.38%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAI+
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIA 160

[Close]
BLAST of Moc06g03210 vs. ExPASy TrEMBL
Match: A0A5D3DXV7 (Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00110 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 4.4e-78
Identity = 155/160 (96.88%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQT+LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSA++FLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of Moc06g03210 vs. ExPASy TrEMBL
Match: A0A6J1L5D2 (probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC111499274 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 4.4e-78
Identity = 155/160 (96.88%), Postives = 158/160 (98.75%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAI PDFNEE+MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIRPDFNEEIMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFAS+MAKSAS+FLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASIMAKSASDFLGLAIS 160

[Close]
BLAST of Moc06g03210 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 245.7 bits (626), Expect = 2.4e-65
Identity = 120/154 (77.92%), Postives = 142/154 (92.21%), Query Frame = 0 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFD 66
           +Q+ QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVL+A+MD+NGNG VEFD
Sbjct: 3   DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFD 62

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV  I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM
Sbjct: 63  ELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEM 122

Query: 127 MKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           +KEADT+GDGVISF EFAS+MAKSA ++ GL I+
Sbjct: 123 IKEADTNGDGVISFGEFASIMAKSAVDYFGLKIN 156

[Close]
BLAST of Moc06g03210 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 240.4 bits (612), Expect = 1.0e-63
Identity = 120/154 (77.92%), Postives = 137/154 (88.96%), Query Frame = 0 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFD 66
           +Q+ QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +L+  +D NGNGSVEFD
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEM
Sbjct: 68  ELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEM 127

Query: 127 MKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 161
           M EAD++GDGVISFNEF+ +MAKSA++FLGL  S
Sbjct: 128 MTEADSNGDGVISFNEFSHIMAKSAADFLGLTAS 161

[Close]
BLAST of Moc06g03210 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 158.7 bits (400), Expect = 3.9e-39
Identity = 86/150 (57.33%), Postives = 99/150 (66.00%), Query Frame = 0 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNG 61
           A L  EQL +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG
Sbjct: 11  AKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNG 70

Query: 62  SVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYR 121
            VEF E V  + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  
Sbjct: 71  LVEFSEFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAE 130

Query: 122 ELTEMMKEADTDGDGVISFNEFASVMAKSA 152
           ELT M+KEAD DGDG I F EF   +  +A
Sbjct: 131 ELTGMIKEADRDGDGCIDFQEFVQAITSAA 159

[Close]
BLAST of Moc06g03210 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 151.0 bits (380), Expect = 8.2e-37
Identity = 80/149 (53.69%), Postives = 99/149 (66.44%), Query Frame = 0 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFD 66
           EQ+N+LR+IF  FD + DGSLT LEL +LLR+LG+KPS DQ   L+   D   NG VEF 
Sbjct: 12  EQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEFP 71

Query: 67  ELVTAIMPDF----NEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRE 126
           E V  + P+            + QLL +FR FD DGNG+ITAAELA SMAK+G  LT  E
Sbjct: 72  EFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVAE 131

Query: 127 LTEMMKEADTDGDGVISFNEFASVMAKSA 152
           LT M+KEAD+DGDG I+F EFA  +  +A
Sbjct: 132 LTGMIKEADSDGDGRINFQEFAKAINSAA 160

[Close]
BLAST of Moc06g03210 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 118.2 bits (295), Expect = 5.9e-27
Identity = 55/145 (37.93%), Postives = 93/145 (64.14%), Query Frame = 0 

Query: 3   ALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGS 62
           AL  +Q+ + ++ F  FD D DG +T+ ELA ++RSL   P+  ++H ++  +D++ NG+
Sbjct: 5   ALTKDQITEFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGT 64

Query: 63  VEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRE 122
           +EF E +  +     E     + +L E F+ FD+D NGYI+A+EL+  M  +G+ LT  E
Sbjct: 65  IEFAEFLNLMAKKLQESDA--EEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEE 124

Query: 123 LTEMMKEADTDGDGVISFNEFASVM 148
           + +M+KEAD DGDG ++++EF  +M
Sbjct: 125 VEQMIKEADLDGDGQVNYDEFVKMM 147

The following BLAST results are available for this feature:
BLAST of Moc06g03210 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022135250.11.3e-79100.00probable calcium-binding protein CML15 [Momordica charantia][more]
XP_004138337.23.1e-7897.50probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probabl... [more]
KAG6589571.13.1e-7898.13putative calcium-binding protein CML15, partial [Cucurbita argyrosperma subsp. s... [more]
KAE8652452.13.1e-7897.50hypothetical protein Csa_013816 [Cucumis sativus][more]
XP_022921807.13.1e-7898.13probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023516651.1 prob... [more]
BLAST of Moc06g03210 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FZ753.4e-6477.92Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ... [more]
Q9LI841.4e-6277.92Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ... [more]
Q6L5F41.7e-5571.71Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5ZD814.8e-5064.20Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9M8U15.5e-3857.33Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 ... [more]
BLAST of Moc06g03210 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1C0M66.1e-80100.00probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A0A0LUS71.5e-7897.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1[more]
A0A6J1E1G51.5e-7898.13probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC11142... [more]
A0A5D3DXV74.4e-7896.88Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1L5D24.4e-7896.88probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC1114992... [more]
BLAST of Moc06g03210 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G18530.12.4e-6577.92EF hand calcium-binding protein family [more]
AT3G25600.11.0e-6377.92Calcium-binding EF-hand family protein [more]
AT3G03000.13.9e-3957.33EF hand calcium-binding protein family [more]
AT1G32250.18.2e-3753.69Calcium-binding EF-hand family protein [more]
AT4G14640.15.9e-2737.93calmodulin 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 11..39
e-value: 0.046
score: 22.9
coord: 122..150
e-value: 8.4E-8
score: 41.9
coord: 86..114
e-value: 5.7E-4
score: 29.2
coord: 47..75
e-value: 3.1
score: 13.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 85..147
e-value: 3.5E-15
score: 56.2
coord: 10..72
e-value: 7.1E-11
score: 42.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 82..117
score: 14.039183
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 7..42
score: 13.481276
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 118..153
score: 14.039183
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 43..78
score: 9.018011
IPR002048EF-hand domainCDDcd00051EFhcoord: 86..148
e-value: 6.59054E-19
score: 73.7361
IPR002048EF-hand domainCDDcd00051EFhcoord: 11..73
e-value: 1.00691E-12
score: 57.9429
NoneNo IPR availableGENE3D1.10.238.10coord: 104..157
e-value: 8.6E-16
score: 59.6
NoneNo IPR availableGENE3D1.10.238.10coord: 1..103
e-value: 9.8E-27
score: 95.3
NoneNo IPR availablePANTHERPTHR23050:SF351CALCIUM-BINDING PROTEIN CML15-RELATEDcoord: 3..159
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 3..159
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 95..107
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 131..143
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 56..68
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 20..32
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 4..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g03210.1Moc06g03210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030234 enzyme regulator activity