Moc06g03140 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g03140
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	protein MIS12 homolog
Location	chr6: 2229023 .. 2231519 (-)
RNA-Seq Expression	Moc06g03140
Synteny	Moc06g03140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAAGTAAGGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATCTTGTCGACGATTTGGTCGATGACGCTTTCGATTTTTATCAATTGTATTGCTCTCTGTCTCGCATCGTCTCTTCAATTTAGTTTTTCAGTTTTGTTCGGTTAATTTGATTCTTGCGAATTCTTGTTACCTTCAGACAAGCATCAGCCACCTTGAAGACCGAGGGCGTGGATAAATCCCAGGATCTTATCATGGTGAGGTTTGAGTTATATTTCTGTCTTCCGATTGGTTAAGTTGGTACTCTGAAGCATTAGTTTGGAATGGGCACAATTCTCTTTGAATAAAATTGTTTATTTACCTTGTTCGTGTAGGGCATATCGCGCGTCCGATCGTTCGTCCATTCAGTTTTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTGAATCACTGTTTTGCGGTCCCTGAAGGCTTTTCTTTACCCTCAAATGTAGGTTCTTCTATTCTATCTGTGATGAGGCCCTTACACTTTGTTACGTGAATTCGTTCTATTATTGTTGTTTTTGTAATTGAGTTCGTTGCCCTTGCTGTTCGAATCACTTCATAAGTGATTTATATTGAGCATGTAAGACGGCTACTTGCTTATGCAGCGAGTTGCCCACTTATACTGACTTGAGAACTTCCTCAATTAATTTTTAAAAAAGGCTCTATTACTATTTCATCATTGAGTTTCAAACTTTTGCAACATAGATTCCTCATTTTGATTGCTATATCTTAACGTGGTTCAGTTGATACTGGTCGTGACTGTATAAGATTTTCTAACTCTAAAAGTATTGTAAACAAGTGCCTGCTGCATTTTTTAGAAAAACACTGTGGTGTATCATGCTCCTTTTTCTTTTTTTCCCTTTTTTTGTTTTGTCAATCATATCTGAATCCTAACCTCTGTGAGGTCTAGTTCGACTCCTTTTGTTGTGCATCAATATAGTTGCGTGGGTTAGCAGTGTTTCTGTCTGTTTCACTGGGATCTCATTCGAAGCCTTGACTATTGTCAAGTTGCTAAATTGTCGATGGTTCCCTTATCTTGTTTGAGAACTTTGAGTTATGGTTAATTGAGTCTATGTATTCTCTTATTATTTTTCTTCTTCCTATGCAACAAACCCATTTCTAATTCAGATCGAAATATTTTCCTTAAGTTGTCTTAGGATGAATCACCTGCCGACACTTCGATAAGTCATGATTGCCTCTATGACGTGGGTCTGGATACAGAGTTAGATTCTTTGAGGAACAGGCTCTCTGAGGTAATAATTGTGCCGCATGTTTTTTACTTCTCATGTTTCTGTACATAATATTTCATAAGAAAACATATTTTTCAGGTCAGAGAAGAGAATGTTGTATTGAACCAAGAACTCCAAGCTTTAGAAAGGCAAACTGCTTCCAGTAACTCACAAATCAATCATTTCAACGAGGCATTAGAATTATACGAGGAGACTTCAGTGAATGAGAGGTTCCAGGGTAATTAAGTTGTGGTTAAAAATCGTTGTTTGCTCGTGCACTGTTAGATATCTTATAAATAATTAAATTTACCCTAACCCTTCAAGTTAAACTTTTGGATTTATTGGTAGGTTAATCATGTTTAAAATGCACTCCTGGGCTTACATATGCATTATCCTAGTAGTATACTCATGAAGGCTGACCCATAATGTCATATGCAATTAACGTATGAAGCTGACTTATTTTATTTTTTCTTATAAGGAAGTTTTATTGTCAGAATTGGCTTGGTAGACTAAAATTTCTGTAAACACTTAACTTTCTGCTCTGAAGGATCTTATGGCCTGCATTTCTCCTTGTGACGCAAACTGTGATACATACAAAAGAATTTCAGTGAACATCTGCTAAATCGTTACGTTCTGGTTTCATTTGGGCAATTTTATTTTACATCAAGGAATAGATTTAGTGGATATTCCTTTTTGTTTCCCCTTTTCAATTGCTCATTGCGTAAAAATGGAACTTTAGTCTGTGAATTCTGTAGTTTCTCTAGCTATTTTCACTCTAAAGGTCACTCGGGACAAAACTTACTAATTTGCATAAATTTTCATTGTACCGAAATTTTAACAGTGGACAAATAAAACCTGTTCATGATTCATGGAGATAAAATTCTAACATATATCAAATTTCTGCAGAAGTGATGAACACTGCTTCGGAGCTCCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATAGAGGAATCCAAGCTCACTAAAGTAGAGAAGGTTCATACAAATGGCGACATGTCTCACCATCACAAGGGTAAGATTTTCAAGCTACTTCACGCATCAATCAAGTTGGTTTTAGCTTTGTGGGAAGGCGAATACAATATTGTTCCACATGATATTTGATGCTTGTTTCTGCTCTGCAGGCTTCTCTAATGCTAAGCTTGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGA

mRNA sequence

ATGGAAGGAAGTAAGGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATCTTGTCGACGATTTGGTCGATGACGCTTTCGATTTTTATCAATTACAAGCATCAGCCACCTTGAAGACCGAGGGCGTGGATAAATCCCAGGATCTTATCATGGGCATATCGCGCGTCCGATCGTTCGTCCATTCAGTTTTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTGAATCACTGTTTTGCGGTCCCTGAAGGCTTTTCTTTACCCTCAAATGATGAATCACCTGCCGACACTTCGATAAGTCATGATTGCCTCTATGACGTGGGTCTGGATACAGAGTTAGATTCTTTGAGGAACAGGCTCTCTGAGGTCAGAGAAGAGAATGTTGTATTGAACCAAGAACTCCAAGCTTTAGAAAGGCAAACTGCTTCCAGTAACTCACAAATCAATCATTTCAACGAGGCATTAGAATTATACGAGGAGACTTCAGTGAATGAGAGGTTCCAGGAAGTGATGAACACTGCTTCGGAGCTCCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATAGAGGAATCCAAGCTCACTAAAGTAGAGAAGGTTCATACAAATGGCGACATGTCTCACCATCACAAGGGCTTCTCTAATGCTAAGCTTGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGA

Coding sequence (CDS)

ATGGAAGGAAGTAAGGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATCTTGTCGACGATTTGGTCGATGACGCTTTCGATTTTTATCAATTACAAGCATCAGCCACCTTGAAGACCGAGGGCGTGGATAAATCCCAGGATCTTATCATGGGCATATCGCGCGTCCGATCGTTCGTCCATTCAGTTTTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTGAATCACTGTTTTGCGGTCCCTGAAGGCTTTTCTTTACCCTCAAATGATGAATCACCTGCCGACACTTCGATAAGTCATGATTGCCTCTATGACGTGGGTCTGGATACAGAGTTAGATTCTTTGAGGAACAGGCTCTCTGAGGTCAGAGAAGAGAATGTTGTATTGAACCAAGAACTCCAAGCTTTAGAAAGGCAAACTGCTTCCAGTAACTCACAAATCAATCATTTCAACGAGGCATTAGAATTATACGAGGAGACTTCAGTGAATGAGAGGTTCCAGGAAGTGATGAACACTGCTTCGGAGCTCCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATAGAGGAATCCAAGCTCACTAAAGTAGAGAAGGTTCATACAAATGGCGACATGTCTCACCATCACAAGGGCTTCTCTAATGCTAAGCTTGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGA

Protein sequence

MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLDTELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

Homology

[Close]

BLAST of Moc06g03140 vs. NCBI nr
Match: XP_022134693.1 (protein MIS12 homolog [Momordica charantia])

HSP 1 Score: 469.9 bits (1208), Expect = 1.3e-128
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM
Sbjct: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD
Sbjct: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV
Sbjct: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 416.4 bits (1069), Expect = 1.7e-112
Identity = 210/241 (87.14%), Postives = 226/241 (93.78%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVR+ENVVLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.0 bits (1068), Expect = 2.2e-112
Identity = 210/241 (87.14%), Postives = 226/241 (93.78%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QASA+LKTEG D+SQDL +
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVR+ENVVLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. NCBI nr
Match: KAG7023263.1 (Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 413.7 bits (1062), Expect = 1.1e-111
Identity = 209/241 (86.72%), Postives = 224/241 (92.95%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVR+ENVVLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. NCBI nr
Match: XP_022921372.1 (protein MIS12 homolog [Cucurbita moschata])

HSP 1 Score: 412.5 bits (1059), Expect = 2.4e-111
Identity = 208/241 (86.31%), Postives = 223/241 (92.53%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVR+EN+VLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 4.7e-57
Identity = 126/247 (51.01%), Postives = 173/247 (70.04%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVD--KSQDL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +G D  KSQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVG 120
             GI+RVR  + SV+D RL +WE Y L  CFAVP+GF LP ++ES   +S+  D LYD+ 
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ 180
           LD ELDSLR++L+ V + +V L+ ELQALER + S    +   NEALELY+E+S++E F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EVMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEF 240
           E+   ASELRA + +LK RR++ S+  KV++      + +H K FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

Query: 241 LADLKTL 242
            A+L+ +
Sbjct: 241 QAELRKM 238

[Close]

BLAST of Moc06g03140 vs. ExPASy TrEMBL
Match: A0A6J1C2Q6 (protein MIS12 homolog OS=Momordica charantia OX=3673 GN=LOC111006900 PE=3 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 6.2e-129
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM
Sbjct: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD
Sbjct: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV
Sbjct: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.2e-111
Identity = 208/241 (86.31%), Postives = 223/241 (92.53%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVR+EN+VLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 2.0e-111
Identity = 208/241 (86.31%), Postives = 224/241 (92.95%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LR  LSEVR+EN+VLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTN D+SHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Moc06g03140 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 5.0e-110
Identity = 208/242 (85.95%), Postives = 224/242 (92.56%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ-E 180
           TELD LR  LSEVR+EN+VLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQ E
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQAE 180

Query: 181 VMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLK 240
           +M TASELRAKIGKLKKRR+EESKLTKVEK+HTN D+SHHHKGFSNAKLDDIQEFLADLK
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLK 240

Query: 241 TL 242
           TL
Sbjct: 241 TL 242

[Close]

BLAST of Moc06g03140 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.5e-103
Identity = 196/241 (81.33%), Postives = 220/241 (91.29%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ QASATLKTEG D+SQDL +
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GIS+VR+ V   LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TSI+HD   DV LD
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHD--QDVDLD 120

Query: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVR+EN+VLNQELQALERQTASSNSQI+HFNEAL+LYE++SVN+ FQE+
Sbjct: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           + TASELR+KIGK+KKRR EE+KL KVEKVHTNGD+SHHH GFSNAKLDDIQEFL+DLK 
Sbjct: 181 IKTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKK 239

Query: 241 L 242
           +
Sbjct: 241 I 239

[Close]

BLAST of Moc06g03140 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 222.6 bits (566), Expect = 3.3e-58
Identity = 126/247 (51.01%), Postives = 173/247 (70.04%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVD--KSQDL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +G D  KSQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVG 120
             GI+RVR  + SV+D RL +WE Y L  CFAVP+GF LP ++ES   +S+  D LYD+ 
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ 180
           LD ELDSLR++L+ V + +V L+ ELQALER + S    +   NEALELY+E+S++E F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EVMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEF 240
           E+   ASELRA + +LK RR++ S+  KV++      + +H K FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

Query: 241 LADLKTL 242
            A+L+ +
Sbjct: 241 QAELRKM 238

The following BLAST results are available for this feature:

BLAST of Moc06g03140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134693.1	1.3e-128	100.00	protein MIS12 homolog [Momordica charantia]	[more]
KAG6589575.1	1.7e-112	87.14	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515499.1	2.2e-112	87.14	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG7023263.1	1.1e-111	86.72	Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022921372.1	2.4e-111	86.31	protein MIS12 homolog [Cucurbita moschata]	[more]

BLAST of Moc06g03140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	4.7e-57	51.01	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of Moc06g03140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2Q6	6.2e-129	100.00	protein MIS12 homolog OS=Momordica charantia OX=3673 GN=LOC111006900 PE=3 SV=1	[more]
A0A6J1E0A1	1.2e-111	86.31	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JME3	2.0e-111	86.31	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1JD43	5.0e-110	85.95	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A5A7UQ95	1.5e-103	81.33	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]

BLAST of Moc06g03140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	3.3e-58	51.01	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 123..157
None	No IPR available	COILS	Coil	Coil	coord: 177..197
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 11..141 e-value: 1.2E-18 score: 67.5
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g03140.1	Moc06g03140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus