Moc06g02000 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g02000
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionIsochorismate synthase
Locationchr6: 1433581 .. 1440455 (-)
RNA-Seq ExpressionMoc06g02000
SyntenyMoc06g02000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCAGTTGGCGGAGGTGGGCTGAGTTTGGGCCATTGTTCCAAGGATGGAGCAGAATCGGTCAGAATTGCTTCTCCTCATCAAGTTTCCACTACCGGACGGTGTCTTCGGTTTCGCCCCCATGTGAGTCGCCGGCCAGTTGCGGTGTCTTCCGCCGTTCATCTCTTTTCTGCCTCTAACAATGTGAACTGATTTGTTGGCAGGGGCGGCGGCGGGTGTGTTCTGAGTCGTCGTCAATGGATGGGTGCCGGGGGGAGCCCGAAGTGCCAGTCGGAACAATCGAGACACGGAATCTAGCGGCAGCTCCGACGGCGAAATTGGCGATGGAGAGTCTGAGGGCGGGGGTGTGGAAGTTGAAATCGGAGGCACCGCCGGCGTTTGGTTCAGGGATTGTTCGGCTCCAAGTACGCCTCCAAATCTCTTTCGATAGTTCCTTCACCCATAGATTTTTCTTATCTCTTCCCTCTAACTTCTAAGTGGGCTACTGCCATCTGGCTGTAACGTTAGATTAGATTATTGCATTTCTTATCTTGCCCGGATAGAATGACACTAAACCCCAATTATTAGGAGTGCTATTGGGACTGAGGAAGCTAACTAACCCTTCTAATTAGTTGAGAATTTTGTGGACTTCATTTCATTTTCCATTTTAGAAAAGAAAAAAACGAGGTGGAGACGAGGAATAGGGTGGGAGCCAGGACTTATCCCCACCTCCGCCTCACCGAGGAGCCTAAACGGAGATTTTCTGTCCTTGCTCTAGTCAACATCCTTACTAATTATGTCGATTCCCTACCTTGTTCTAGTCAACGTCCCTACTAATTATGTCGTTGATAAGATGTCATGAGAAATGGAAGAGGCTTTCAATTTTATGTGATTGGCTAAAAAATGGAAGAAAACTTGTCTTTTCACGTCATTAAAATCACTCCACTTTATTCTTTTGACCCACCCTTTAGGTTGTAGCATGCAGGTACCATTGAATCATATTTTAAAGTAATGATGTAATTAATGTCATAATCATTTTTTAGTTTAACGTGTAGTGGTGAGAATCTCAACTTACAATCTTTTAGTCGAAAATAGGTATCTTGACCGTTTAAATTGTATGTCTCAACGAGTTTAAAACTTCCGATTTGTTTAAGTTTATACTCACCGGAATGAAATTGAATAGTATCATTTTGTGGGCATTGACTTTTTTGTTGTGTTGGATATGTTTTAGTTGGATTTCAACAAATTTTTTTTCTTTTTTGTTTATTGGATGTTAAGCTTGGGTATTTATGGTTCAATTAGGGCGTGATCTTGAGTTTTTTTTGATGTTGAATTTCACTGGATTTATTGGTTTGTTGTTGATTGATCTTTTGATTTGGTTTCGAAGTGGTATTAATTGTACCATTTATTTAAATTTAGGATTTAATTTGATGAAATCGAAAGTATAAGGTATAGATAGACAAATTAAATTAATTTGAGCATAGTTCAACTGGTTAAGGTATATGTTTGTGACAAAAGGGTCGTGAGTTTGAATTTCTACCCTCACTTGTTGTTTTATTAAAAAATAAAATAAGAAAAAGACAAACTAAGGTTAGTATAGGGGTAAAATATAAATTTTTTTCAAACATTTTTTACTATTTAAAAAGTCACGGAAACAGATTAAATATTTAAAACTATAGGAATGGATGAAACCTAACCTACACTCCTTATAAAAAGTCTTCATCTTTGTATTTGGGAAGAAAACTTTGCACTATGTTGGAAGTAAACTTTCATAAATTACAGGTCACTCAAATATATAAATTTAGTCCCCTAATCCAGCCTTTGTATCATCACTTGAAAGTTCTACAATTTTAGTTTTTCCCTGCTTCTCCTTTATTTTAGCTTAAATTGTAAGCTTAGTCACTGAACTTGGTCGGACTAGATGTCGTGTCTATCTAGTCCATAGTCTATATACTTTAAAATATTTTTAATAGGTCTCTAAATTTTTAGAGTTATGCCTATTCAGTCCATATCCTTTAAAAATATTTTAATGAGTTCTTGAAACTTCAATTTTGTGTCTAACTACTATTATAACAAGCCAATGTGATTTACTGAAGAAAAACAACAAAAAAAACAAGCATGAAACTGTCTAAAATCAATCCACACACCTGTAATCCCTAAATAGTATAGTCAATGTGTGTCACATTAATGACAAAATATAAACTTTATAACAAAGATTCGTTAGACACAAAATTTAAACTAAAATGACTAGATTAACAAAATCTTGAAAGTTCAGTGATGCATTAGAAATTTTTTAAAATATAATATTTGAATAGACACAAGTATACAAATTTAGGATCTAATCTATAGTTTTTATCTATATTGTTATTATCGAAAAAGTGAGTATAGCTCCATGAACTCATTGATATGTTGTACTTGGGAAAAAAAATCTACATGGTAATTATCTTAGAAGGATTTCCATTTGCATTCTTTCCAATAAAGTATTTAAATTTTCACGTGGCACCTATAATGCTATATTTCATTTAGTGAAACGTATATATATTAATGAGCTCTAATTATCTATAAATAGACTTAAATATGGGTTTGACTGCAGAATTTCTTTCTTTTAAAAATTCTCCCTTCAAATTTTAACCACTCTCAATTTTTCTCAATATCTTTGATGGTTTTTATAAGAAAACTCAACGACTCGAAATCCACTGACCTCATCCAAATTGCATGTGTTTGGTTGGGTTAGATTTTTTCGTTTTACAAGTTCTCTTAAAATTAGTTTTGATTGAATCAAAGTAATCCAAAATTATTTTTAATTAAAAAGTATCTTTTAGTTAAGATAAAATTACATGGACATATATCTATTTTAGTTTTTTTTTTTATACTATACTATTTTAGTTTTACTTCATTAAATACTTCATTTTATATTTTTCTAGTACTATTTGTTCCTACCAAATCCTAAACAAATAATTCGAACAACTGAAATTGATCCAATTCAAATATAACAAATTTGGATTGAATTCATCATCTAACAATGATGATTTTGGTCTAATAAATTTGCATCCGGGATAATTGAGTTGGATGAAAAAAATCTCTTGACCTTGGAATTGTTCGTACAAAGTAATTTTTTTAATACATCAATATGTGAGGAGGATTCAAATCACGATCATATAATTATAGATATATATGTTAGAGCCTATAGTTTAAATCTCATATACAATACAACATTTAAACAAAAAGTTAGAACAAAAATAAACCACATTTAAAAATATTTAGAGACTAAAAGTAAAGTTCTATAATTTAGTAATAAAAAATCAAAAGAAGTTTGATATATTAAAATAATTTTGAAACCTAAGTTGCATTATTTATCTGTACGGTGGTTCAGCTTTGGAAGTTGAGTTCGAGATTCTTTCTTCTTCCCTGAATAGTGAATACACCCATTATCTGTTTTTGCCGCGAAAGATAACTACTTTTTGAGACTGAAGGAGATCTTTTTGAAGGTGCCAATTCAACAGCAAATTCAAGCAATTGATTGGCTTCGTTCTCAGCCCCAGATTCTTCCCCGCACCTTCTTCTCCGGCAGAAGCAGAACCCTCGCTTCTGGCCCTCTCTCCGCCGACAGCATTTACAATGGCCACCATCCCTTCAACCACCACCGCCTCGTCAGCGTCGCCGGTGTGGGCTCTGCCGTCGTCTTCTGTCGCCTTCATCAGTTTTCATACACCGATTGGACGTCCATAAAGAGGTAAACTTATTTTGCCTGGAGTTTTATTCTGCCATCTGTTCGATAAAATGCCTTCGAGGGTCATGTTCTCTAAATTATAAAATTGAAGGTTCCTGTCAAAGAAATGCCCCTTGATTCGTGCTTACGGAGCTATTCGCTTTGATGCACGGGCCAACGTATCGTCTGAGTGGGAGCCGTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTAGCGATTATGTTTTTTAAAATCTCGTTTATCACGCAACTGGCTAATGGGTCGGTGGGCTACGTCTTCTTTATAGAGCGTATTTTAATTGTTCTCAACTGGATACAAAAATGGCAGGTTGAGTTTGATGAATTCGAAGGAAATTCGATGCTCGCTGCTACGGTTGCTTGGGATCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCCCTTCAATCGACAATCCGACAGGTTCGTTTATGTGATACAGTTTGATTCTCTGTGCATTGTATTGAAAGGAATAATATTTTCCCATAGACAATTCCTTGCTTTTAAATGTTTTGTAATTCAGGTTTCTTCTAATATTGTCAAACTACAACGAGATCTTCCAGAGGTATTCATACTTAGGCACAATCACGTTCCCAGCAAAACATACTGGGATCTTGCAATTCACAGAGCCTTGCAAATGATTGACAGAAGCAACTCAGAACTCACGAAGGCATTCACCACTGATTTTCTCTCTCGCTCTCTGTCCACAGGAATGTACAAATTTCATATTTTCTTTTTCTATTCGCCATCATAATTGGTTTTAATGTTATTTACTAAATTTAGGTTGTACTTGCACGTAGTACCCGAATAGTGACGAGTGTCGATATTGATCCTGTTGCATGGGTAGCATGTTTACAGGTAGCCTATATGATGGTACTTCAATTCTCTGTGAATATTACGTTTTTTTAGTTCAACAATGTGAGAAGTGGATATTTGAACATTTAACTTTTTAAAATAGATGTATAATACCTTAATTAGTTGAGTTATGCTTTTTAAAATAGATGTATAATACCTTAATTAGTTGAGTTATGCTCCTTTTTATGCAGCAAATTTGTTTCTTTCTGAATCTAGTTTTAACCGTATCAGGTAGAGGGAAAAAACTCTTATCAGTTTTGTATCCAGCCATCTAATGCTCCAGCCTTCATAGGTAGCACGGTAAGTTTGATTTGTTATCTTGTTATAATATTGCGTATGCTTCTTTTATCTCAGTTACTGTTTCATTTTGTGACAAAATTTGAAGAACCTTCTGTGATGAATTGGAGATTTGTTTGTTATGAAATGGAGAACGTTCGAAAGTAACTTGAACTCTTGTAATACAGTGCCGAAGGTTGGATATTAATGAAAGATGTTTTCAATTAGTTTTCTACTCGTCTGGCTTATACCAAGCGTCCATTTTGTAACAAGTTTAACTACAAATTCGTGGAAATGTTAACCCTTCTTTTTTTAATTGTTTACTAATTGTTTTGACTTGGATATATTTGTAACAGCCTGAGCGACTATTCCATAGAGAAGGACTTAGCATTAGAAGTGAAGCCTTGGCTGCAACTCGTGCGAGGGGCAGATCAGCCATTGAAGACCTTAAAATAGAAAATGATTTGCTTTCCAAGTTAATTTCATCTCTAAATTCTTATTTTTTTTTACTCAATGGTGCTATTATCCTGTTGTCTCAAACAAATGTGCAGAGGACAGGAGTTTCACTAACTCTACTTTCTATCTGCAGCCCAAAGGATGACCTCGAGTTTTCTATTGTGCGGGAAACCATGAAGAAGAAGTTAGAGGTAAGTTTGAGCGTGTCTTGCTACCTTCTTTTCTGTTGATGACATCAAAGAAACTTAAGTGGCATGTTATTGTGCCCTGATAAGCCCCCTATTATAACAAGTTTTGTACTTAGTTGCTATGTGCTACCATGATATTTGTAGTGCTTCCAAACAATGAATTGTTTCTCTTTTATATCTTCGTATTTATGTGCTAAACATTATTACATGCACTTCACCATAAGTCCCGCAAGAATGAAGAGAGGACAATATTACAAGAATATTGCAGATTTTTAGCAGCCTAACACCTATCTCCTAGTTCCAAATGGTACGAGAAATTTGAGTTGTTACCTAAATGATGTTTGTAGGCTGTATGCGATGCAATAATTGTTGAACCACATAAAGCAGTACGAAAACTTCCAAGAATACAACATTTGTATGCCCAACTGGCAGGCAGGCTGAAAAGCGAGGAGGATGAGGTAAAAATCACCAAAAATTATGTTCCCTGTAAACTATTGTTCCCCGACGAGAACATACAGTTCTAACTCTTCTTTGCTGCTTAAAAAGTTTGAAATTTTATCAACACTTCATCCAACTCCCGCCGTTTGTGGATTGCCAACAGAAGAGGCTCGACACTTAATTGCAGAAACTGGTAGGTGAACTTTGGCTGTAAGATTCATGGGGTTGCCATACTAGATTTTTACTCTTGCTCGTAAGTGAACATACGAATAGGTTAGGTCGGTTTGTTCTCTCTATAAACTGCTAGAGGTACTTTATATTATCTCGCCCAGTTTCATCAATGGTTTTCTTCAAATGCTGATTTGGGGTCTTTAATTTTATCGTATGCCAAATCTGGGATTGAAAAGACGAGCTATCTTCACTCTTCACCCGTCTGCTTGCATTTAAGAAACTTGGGAAATTTTATGGTGCAGAAATATTTGATAGGGGGATGTACGCTGGGCCTGTTGGTTGGTTTGGAGGAGGACAGAGTGAGTTTGCTGTGGGCATCAGATCAGCATTAGTGGAGAAAGTAAGATGGCATCTACCATATGAGCTAAACAAACTTAAATACTTGCGTTGCATTTTTACTCTTTTAAATTTTCTGAAACCTTGACGTTGCCATAACCATTCTTTGATTTCAGGGTCTTGGTGCCTTGATCTATGCCGGAACAGGGATAGTAAAAGGAAGCGATCCGTCTTCAGAGTGGGATGAACTGGAGCTCAAAATATCTCAGGTATTCATCATTCAAAATGGAACGTACATTATTTAGTCTTGAAAATGCTCATTTGTAACTCAATTTTAGTTCCACATAACTTTGATCTGCATATTTGTAGGTCACCAGGTCGCTTAATCTAGAAGTGGCACAAGCACCCAAAATTGATTGGTGA

mRNA sequence

ATGGCAGCAGTTGGCGGAGGTGGGCTGAGTTTGGGCCATTGTTCCAAGGATGGAGCAGAATCGGTCAGAATTGCTTCTCCTCATCAAGTTTCCACTACCGGACGGTGTCTTCGGTTTCGCCCCCATGGGCGGCGGCGGGTGTGTTCTGAGTCGTCGTCAATGGATGGGTGCCGGGGGGAGCCCGAAGTGCCAGTCGGAACAATCGAGACACGGAATCTAGCGGCAGCTCCGACGGCGAAATTGGCGATGGAGAGTCTGAGGGCGGGGGTGTGGAAGTTGAAATCGGAGGCACCGCCGGCGTTTGGTTCAGGGATTGTTCGGCTCCAAGTGCCAATTCAACAGCAAATTCAAGCAATTGATTGGCTTCGTTCTCAGCCCCAGATTCTTCCCCGCACCTTCTTCTCCGGCAGAAGCAGAACCCTCGCTTCTGGCCCTCTCTCCGCCGACAGCATTTACAATGGCCACCATCCCTTCAACCACCACCGCCTCGTCAGCGTCGCCGGTGTGGGCTCTGCCGTCGTCTTCTGTCGCCTTCATCAGTTTTCATACACCGATTGGACGTCCATAAAGAGGTTCCTGTCAAAGAAATGCCCCTTGATTCGTGCTTACGGAGCTATTCGCTTTGATGCACGGGCCAACGTATCGTCTGAGTGGGAGCCGTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAATTCGAAGGAAATTCGATGCTCGCTGCTACGGTTGCTTGGGATCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCCCTTCAATCGACAATCCGACAGGTTTCTTCTAATATTGTCAAACTACAACGAGATCTTCCAGAGGTATTCATACTTAGGCACAATCACGTTCCCAGCAAAACATACTGGGATCTTGCAATTCACAGAGCCTTGCAAATGATTGACAGAAGCAACTCAGAACTCACGAAGGTTGTACTTGCACGTAGTACCCGAATAGTGACGAGTGTCGATATTGATCCTGTTGCATGGGTAGCATGTTTACAGGTAGAGGGAAAAAACTCTTATCAGTTTTGTATCCAGCCATCTAATGCTCCAGCCTTCATAGGTAGCACGCCTGAGCGACTATTCCATAGAGAAGGACTTAGCATTAGAAGTGAAGCCTTGGCTGCAACTCGTGCGAGGGGCAGATCAGCCATTGAAGACCTTAAAATAGAAAATGATTTGCTTTCCAACCCAAAGGATGACCTCGAGTTTTCTATTGTGCGGGAAACCATGAAGAAGAAGTTAGAGGCTGTATGCGATGCAATAATTGTTGAACCACATAAAGCAGTACGAAAACTTCCAAGAATACAACATTTGTATGCCCAACTGGCAGGCAGGCTGAAAAGCGAGGAGGATGAGTTTGAAATTTTATCAACACTTCATCCAACTCCCGCCGTTTGTGGATTGCCAACAGAAGAGGCTCGACACTTAATTGCAGAAACTGAAATATTTGATAGGGGGATGTACGCTGGGCCTGTTGGTTGGTTTGGAGGAGGACAGAGTGAGTTTGCTGTGGGCATCAGATCAGCATTAGTGGAGAAAGGTCTTGGTGCCTTGATCTATGCCGGAACAGGGATAGTAAAAGGAAGCGATCCGTCTTCAGAGTGGGATGAACTGGAGCTCAAAATATCTCAGGTCACCAGGTCGCTTAATCTAGAAGTGGCACAAGCACCCAAAATTGATTGGTGA

Coding sequence (CDS)

ATGGCAGCAGTTGGCGGAGGTGGGCTGAGTTTGGGCCATTGTTCCAAGGATGGAGCAGAATCGGTCAGAATTGCTTCTCCTCATCAAGTTTCCACTACCGGACGGTGTCTTCGGTTTCGCCCCCATGGGCGGCGGCGGGTGTGTTCTGAGTCGTCGTCAATGGATGGGTGCCGGGGGGAGCCCGAAGTGCCAGTCGGAACAATCGAGACACGGAATCTAGCGGCAGCTCCGACGGCGAAATTGGCGATGGAGAGTCTGAGGGCGGGGGTGTGGAAGTTGAAATCGGAGGCACCGCCGGCGTTTGGTTCAGGGATTGTTCGGCTCCAAGTGCCAATTCAACAGCAAATTCAAGCAATTGATTGGCTTCGTTCTCAGCCCCAGATTCTTCCCCGCACCTTCTTCTCCGGCAGAAGCAGAACCCTCGCTTCTGGCCCTCTCTCCGCCGACAGCATTTACAATGGCCACCATCCCTTCAACCACCACCGCCTCGTCAGCGTCGCCGGTGTGGGCTCTGCCGTCGTCTTCTGTCGCCTTCATCAGTTTTCATACACCGATTGGACGTCCATAAAGAGGTTCCTGTCAAAGAAATGCCCCTTGATTCGTGCTTACGGAGCTATTCGCTTTGATGCACGGGCCAACGTATCGTCTGAGTGGGAGCCGTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAATTCGAAGGAAATTCGATGCTCGCTGCTACGGTTGCTTGGGATCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCCCTTCAATCGACAATCCGACAGGTTTCTTCTAATATTGTCAAACTACAACGAGATCTTCCAGAGGTATTCATACTTAGGCACAATCACGTTCCCAGCAAAACATACTGGGATCTTGCAATTCACAGAGCCTTGCAAATGATTGACAGAAGCAACTCAGAACTCACGAAGGTTGTACTTGCACGTAGTACCCGAATAGTGACGAGTGTCGATATTGATCCTGTTGCATGGGTAGCATGTTTACAGGTAGAGGGAAAAAACTCTTATCAGTTTTGTATCCAGCCATCTAATGCTCCAGCCTTCATAGGTAGCACGCCTGAGCGACTATTCCATAGAGAAGGACTTAGCATTAGAAGTGAAGCCTTGGCTGCAACTCGTGCGAGGGGCAGATCAGCCATTGAAGACCTTAAAATAGAAAATGATTTGCTTTCCAACCCAAAGGATGACCTCGAGTTTTCTATTGTGCGGGAAACCATGAAGAAGAAGTTAGAGGCTGTATGCGATGCAATAATTGTTGAACCACATAAAGCAGTACGAAAACTTCCAAGAATACAACATTTGTATGCCCAACTGGCAGGCAGGCTGAAAAGCGAGGAGGATGAGTTTGAAATTTTATCAACACTTCATCCAACTCCCGCCGTTTGTGGATTGCCAACAGAAGAGGCTCGACACTTAATTGCAGAAACTGAAATATTTGATAGGGGGATGTACGCTGGGCCTGTTGGTTGGTTTGGAGGAGGACAGAGTGAGTTTGCTGTGGGCATCAGATCAGCATTAGTGGAGAAAGGTCTTGGTGCCTTGATCTATGCCGGAACAGGGATAGTAAAAGGAAGCGATCCGTCTTCAGAGTGGGATGAACTGGAGCTCAAAATATCTCAGGTCACCAGGTCGCTTAATCTAGAAGTGGCACAAGCACCCAAAATTGATTGGTGA

Protein sequence

MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLEVAQAPKIDW
Homology
BLAST of Moc06g02000 vs. NCBI nr
Match: XP_022134886.1 (isochorismate synthase 2, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAP 360
           AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAP
Sbjct: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAP 360

Query: 361 AFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETM 420
           AFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETM
Sbjct: 361 AFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETM 420

Query: 421 KKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPT 480
           KKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPT
Sbjct: 421 KKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPT 480

Query: 481 EEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSD 540
           EEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSD
Sbjct: 481 EEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSD 540

Query: 541 PSSEWDELELKISQVTRSLNLEVAQAPKIDW 572
           PSSEWDELELKISQVTRSLNLEVAQAPKIDW
Sbjct: 541 PSSEWDELELKISQVTRSLNLEVAQAPKIDW 571

BLAST of Moc06g02000 vs. NCBI nr
Match: XP_022134885.1 (isochorismate synthase 2, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 571/576 (99.13%), Postives = 571/576 (99.13%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ-----VEGKNSYQFCIQ 360
           AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ     VEGKNSYQFCIQ
Sbjct: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVAYMMVEGKNSYQFCIQ 360

Query: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420
           PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI
Sbjct: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420

Query: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480
           VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV
Sbjct: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480

Query: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540
           CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI
Sbjct: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540

Query: 541 VKGSDPSSEWDELELKISQVTRSLNLEVAQAPKIDW 572
           VKGSDPSSEWDELELKISQVTRSLNLEVAQAPKIDW
Sbjct: 541 VKGSDPSSEWDELELKISQVTRSLNLEVAQAPKIDW 576

BLAST of Moc06g02000 vs. NCBI nr
Match: XP_022134887.1 (isochorismate synthase 2, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 922.5 bits (2383), Expect = 1.7e-264
Identity = 462/467 (98.93%), Postives = 462/467 (98.93%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ-----VEGKNSYQFCIQ 360
           AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ     VEGKNSYQFCIQ
Sbjct: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVAYMMVEGKNSYQFCIQ 360

Query: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420
           PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI
Sbjct: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420

Query: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 463
           VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE
Sbjct: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 467

BLAST of Moc06g02000 vs. NCBI nr
Match: XP_038879640.1 (isochorismate synthase 2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 849.0 bits (2192), Expect = 2.4e-242
Identity = 429/528 (81.25%), Postives = 463/528 (87.69%), Query Frame = 0

Query: 43  GRRRVCSE---SSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPP 102
           GRR V  E   S++MDGC G+    VGTIETR   A  TA LA ESLR GV KLK EA P
Sbjct: 42  GRRWVVGEVCQSAAMDGCGGDGGGTVGTIETRTFPAVQTAALATESLRRGVAKLKLEA-P 101

Query: 103 AFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFN 162
           A  SGI+RLQVPIQQ+IQAIDWL SQP +LPRTFFSGRSR+ A    SA +  NG HPFN
Sbjct: 102 ALTSGIIRLQVPIQQRIQAIDWLSSQPHLLPRTFFSGRSRSRAID--SAHNTSNGSHPFN 161

Query: 163 HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWE 222
           HH L+  AGVGSAVVF RLH FSY DW SIKRFLSKKCPLIRAYG IRFD+RAN+S EWE
Sbjct: 162 HHHLLGAAGVGSAVVFHRLHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWE 221

Query: 223 PFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQR 282
           PFGSFYFMVPQVEFDEFE NSMLAATVAWDHACSWTWENAVESLQST+ QVSSN V LQ+
Sbjct: 222 PFGSFYFMVPQVEFDEFEENSMLAATVAWDHACSWTWENAVESLQSTMEQVSSNNVNLQK 281

Query: 283 DLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWV 342
           DLP + I  + HVPSKT+WD A+ RALQ IDRSNSELTKVVLARSTRIVTSVDIDPV WV
Sbjct: 282 DLPLIPIHSYTHVPSKTFWDRAVDRALQEIDRSNSELTKVVLARSTRIVTSVDIDPVTWV 341

Query: 343 ACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKI 402
           ACLQ EGKNSYQFC+QPSNAPAFIG+TPERLFHREGL I+S+ALAATRARGRS IED+KI
Sbjct: 342 ACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREGLGIKSDALAATRARGRSPIEDIKI 401

Query: 403 ENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSE 462
           E+DL SNPKDDLEFSIVRET+KKKLEAVCDA++VEPHK+VRKLPRIQHLYAQL GRL+SE
Sbjct: 402 EHDLFSNPKDDLEFSIVRETIKKKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLTGRLRSE 461

Query: 463 EDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSA 522
           EDEFEILSTLHPTPAVCGLPT EAR LIAETE+FDRGMYAGPVGWFGGG+SEFAVGIRSA
Sbjct: 462 EDEFEILSTLHPTPAVCGLPTNEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSA 521

Query: 523 LVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLEVAQAP 568
           LV++GLGALIYAGTGIVKGS+PSSEWDELELKISQ+TRSL LEVAQAP
Sbjct: 522 LVKRGLGALIYAGTGIVKGSNPSSEWDELELKISQITRSLKLEVAQAP 566

BLAST of Moc06g02000 vs. NCBI nr
Match: XP_004138153.3 (isochorismate synthase 2, chloroplastic isoform X1 [Cucumis sativus] >XP_011659970.2 isochorismate synthase 2, chloroplastic isoform X1 [Cucumis sativus] >KAE8652412.1 hypothetical protein Csa_013914 [Cucumis sativus])

HSP 1 Score: 836.6 bits (2160), Expect = 1.2e-238
Identity = 425/536 (79.29%), Postives = 468/536 (87.31%), Query Frame = 0

Query: 39  FRPHGRR---RVCSESSSMDGCRG---EPEVPVGTIETRNLAAAPTAKLAMESLRAGVWK 98
           +R  GRR    VC +S++MDGC G   EP   VG IETRN  A  TA LA E LR GV +
Sbjct: 32  WRERGRRWGGEVC-DSAAMDGCVGGSQEPGGTVGIIETRNFPAVQTAALATERLRRGVAE 91

Query: 99  LKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIY 158
           LKSEA PAF SGI+RLQVPIQQ+IQAIDWL SQP +LPRTFFSGRSR   S   SA +I 
Sbjct: 92  LKSEA-PAFTSGIIRLQVPIQQRIQAIDWLSSQPHLLPRTFFSGRSRPTHS---SAHNIS 151

Query: 159 NGHHPFN-HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDAR 218
           NG H FN HH L+SVAGVGSAVVF RLH FSY DW SIKRFLSKKCPLIRAYG IRFD+R
Sbjct: 152 NGSHSFNHHHHLLSVAGVGSAVVFRRLHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSR 211

Query: 219 ANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVS 278
           AN+S EWEPFGSFYFMVPQVEFDEFE NSMLAATVAWDHACSWTWENA+ESLQSTI QVS
Sbjct: 212 ANISPEWEPFGSFYFMVPQVEFDEFEENSMLAATVAWDHACSWTWENAIESLQSTIEQVS 271

Query: 279 SNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSV 338
           SN V LQ+DLP++ IL + HVPSKT+W+ A+ RALQ+ID SNSELTKVVLARST+IVT V
Sbjct: 272 SNNVNLQKDLPQIPILSYTHVPSKTFWERAVDRALQVIDGSNSELTKVVLARSTQIVTGV 331

Query: 339 DIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGR 398
           DIDPV WVACLQ EG NSYQFC+QPSNAPAFIG+TPERLFHREGL I+S+ALAATRARGR
Sbjct: 332 DIDPVTWVACLQKEGNNSYQFCVQPSNAPAFIGNTPERLFHREGLGIKSDALAATRARGR 391

Query: 399 SAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQ 458
           +AIED+KIE+DL SNPK+DLEFSIVRET+KKKLEAVCDA++VEP+K+VRKLPRIQHLYAQ
Sbjct: 392 TAIEDIKIEHDLFSNPKEDLEFSIVRETIKKKLEAVCDAVVVEPYKSVRKLPRIQHLYAQ 451

Query: 459 LAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSE 518
           L GRL+SEEDEFEILSTLHPTPAVCGLPTEEAR  IAETE+FDRGMYAGPVGWFGGG+SE
Sbjct: 452 LTGRLRSEEDEFEILSTLHPTPAVCGLPTEEARDFIAETEVFDRGMYAGPVGWFGGGESE 511

Query: 519 FAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLEVAQAP 568
           FAVGIRSALV++GLGALIYAGTGIVKGS+PSSEWDELELKISQ++RSL LEV  AP
Sbjct: 512 FAVGIRSALVKRGLGALIYAGTGIVKGSNPSSEWDELELKISQISRSLELEVVHAP 562

BLAST of Moc06g02000 vs. ExPASy Swiss-Prot
Match: Q9M9V6 (Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 PE=2 SV=2)

HSP 1 Score: 636.3 bits (1640), Expect = 3.2e-181
Identity = 323/524 (61.64%), Postives = 404/524 (77.10%), Query Frame = 0

Query: 44  RRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGS 103
           R+R    + SM+GC  + + P+GT+ETR L+  P+   A E L   V  LKS+ PP F S
Sbjct: 41  RQRFLRCTLSMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQ-PPPFSS 100

Query: 104 GIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRL 163
           GIVRLQVPI+Q+I AIDWL +Q +ILPR+FFS RS +     L   S  NG    + H  
Sbjct: 101 GIVRLQVPIEQKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS---SDHNP 160

Query: 164 VSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGS 223
           VSVAG+GSAV F  L  FS+ DW SI+RFLS K PLIRAYG +RFD    ++ EWE FGS
Sbjct: 161 VSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGS 220

Query: 224 FYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPE 283
           FYF VPQVEFDEF G+SMLAATVAWD+  SWT ENA+E+LQ T+ QVSS I++L+R+   
Sbjct: 221 FYFTVPQVEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLG 280

Query: 284 VFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ 343
           V ++  NHVPS+  +  A++ AL++I   +S L+KVVLARS+RI+T  DIDP+AW+A LQ
Sbjct: 281 VIVVSKNHVPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQ 340

Query: 344 VEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDL 403
            EG+++YQFC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL
Sbjct: 341 CEGQDAYQFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDL 400

Query: 404 LSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEF 463
           L++PKDDLEFSIVRE +++KL+ +CD ++V+PHK+VRKL R+QHLY+QLAG+LK E+DEF
Sbjct: 401 LTSPKDDLEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEF 460

Query: 464 EILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEK 523
            IL+ LHPTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGG+SEF+VGIRSALVEK
Sbjct: 461 NILTALHPTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEK 520

Query: 524 GLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLEVAQAP 568
           GLGALIYAGTGIV GS+PSSEW+ELELKISQ T+SL  E A  P
Sbjct: 521 GLGALIYAGTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 560

BLAST of Moc06g02000 vs. ExPASy Swiss-Prot
Match: Q9S7H8 (Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 PE=1 SV=2)

HSP 1 Score: 616.3 bits (1588), Expect = 3.5e-175
Identity = 317/529 (59.92%), Postives = 396/529 (74.86%), Query Frame = 0

Query: 39  FRPHGRRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAP 98
           F    R++  S S SM+GC G+ + P+GT+ETR + A  +   A E L + V +LKS+ P
Sbjct: 33  FTRFSRKKYESCSMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQ-P 92

Query: 99  PAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPF 158
           P+F SG+VRLQVPI QQI AIDWL++Q +I PR FFS RS       L   +  NG+   
Sbjct: 93  PSFSSGVVRLQVPIDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNG 152

Query: 159 N-----HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARAN 218
           N        LVSVAG+GSAV F  L  FS+ DW SI+RFLS   PLIRAYG +RFD    
Sbjct: 153 NGTVSSDRNLVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGK 212

Query: 219 VSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 278
           ++ EWEPFG+FYF VPQVEF+EF G+SMLAAT+AWD   SWT ENA+E+LQ T+ QVSS 
Sbjct: 213 IAVEWEPFGAFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSV 272

Query: 279 IVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDI 338
           ++KL+     V +L  NHVP+K  +  A+ +AL+MI++ +S L KVVLAR++RI+T  DI
Sbjct: 273 VMKLRNRSLGVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDI 332

Query: 339 DPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSA 398
           DP+AW+A LQ EG ++YQFC+QP  APAFIG+TPERLF R  L + SEALAATR R  S+
Sbjct: 333 DPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASS 392

Query: 399 IEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLA 458
             D++IE DLL++PKDDLEFSIVRE +++KL  +CD ++V+P K VRKL R+QHLY+QLA
Sbjct: 393 ARDMEIERDLLTSPKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLA 452

Query: 459 GRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFA 518
           GRL  E+DE++IL+ LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG +SEFA
Sbjct: 453 GRLTKEDDEYKILAALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFA 512

Query: 519 VGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLE 563
           VGIRSALVEKGLGALIYAGTGIV GSDPSSEW+EL+LKISQ T+S+  E
Sbjct: 513 VGIRSALVEKGLGALIYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560

BLAST of Moc06g02000 vs. ExPASy Swiss-Prot
Match: Q9ZPC0 (Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 2.7e-172
Identity = 307/528 (58.14%), Postives = 398/528 (75.38%), Query Frame = 0

Query: 48  CSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVR 107
           CS S SM+GC G+P  PVGTIETR L A  T  LAME L + V  LKS  P A  SGI+R
Sbjct: 49  CSTSLSMNGCNGDPRAPVGTIETRTLPAVSTPALAMERLSSAVANLKSTLPSA-QSGIIR 108

Query: 108 LQVPIQQQIQAIDWLRSQPQ--ILPRTFFSGRSRTLASGPLSAD------SIYNGH---- 167
           L+VPI++ I+A+DWL SQ Q  +LPR +FSGRS+   S   S D      S  NGH    
Sbjct: 109 LEVPIEEHIEALDWLHSQDQKNLLPRCYFSGRSQVTFSDFTSNDLTNRNGSAANGHLQRI 168

Query: 168 -HPFNHHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANV 227
               +   LVSVAGVGSAV+F   + FS+ DW SIKRFLSK CPLIRAYGAIRFDAR ++
Sbjct: 169 STSSDDKNLVSVAGVGSAVLFRSPNPFSFDDWLSIKRFLSKNCPLIRAYGAIRFDARPHI 228

Query: 228 SSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNI 287
           + EW+ FGSFYF+VPQVEFDE  G+SM+AATVAWD+A S T++ A+ +LQ+T+ QVSS +
Sbjct: 229 APEWKAFGSFYFVVPQVEFDELHGSSMIAATVAWDNALSLTYQQAIVALQTTMEQVSSTV 288

Query: 288 VKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDID 347
            KL++D+    ++   ++P +T WDL ++R L+ I    S LTKVVLAR ++++T+ DID
Sbjct: 289 SKLRQDVSHTSLVSKANIPDRTSWDLTLNRVLEEIGNKYSPLTKVVLARRSQVITTSDID 348

Query: 348 PVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAI 407
           P+AW++  + +GK++YQFC+QP  APAFIG+TPE+LF R+ L++ SEALAATRARG S  
Sbjct: 349 PLAWLSSFKADGKDAYQFCLQPHEAPAFIGNTPEQLFGRDQLTVFSEALAATRARGESDS 408

Query: 408 EDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAG 467
            DL++ +DL S+PKD+ EF+IVRE +++KL+A+C ++  EP K+VRKL RIQHLYA+ AG
Sbjct: 409 LDLQMAHDLFSSPKDNHEFAIVRENIRQKLDAICTSVETEPMKSVRKLKRIQHLYARFAG 468

Query: 468 RLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAV 527
           RL+SE+DEF+ILS+LHPTPAVCG P E+AR  IAE E+FDRG+YAGPVG+FGG QS+F+V
Sbjct: 469 RLRSEDDEFKILSSLHPTPAVCGFPMEDARKFIAENEMFDRGLYAGPVGFFGGAQSDFSV 528

Query: 528 GIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLE 563
           GIRSAL+ K  GALIYAG G+V+GSDP+ EW ELELK SQ  + + LE
Sbjct: 529 GIRSALIGKDAGALIYAGLGVVEGSDPALEWQELELKASQFMKLMKLE 575

BLAST of Moc06g02000 vs. ExPASy Swiss-Prot
Match: P38051 (Isochorismate synthase MenF OS=Escherichia coli (strain K12) OX=83333 GN=menF PE=1 SV=4)

HSP 1 Score: 157.9 bits (398), Expect = 3.4e-37
Identity = 140/477 (29.35%), Postives = 220/477 (46.12%), Query Frame = 0

Query: 93  LKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIY 152
           L  E P   G  ++ +  P++    A+ WL SQ Q  P+ ++  R+              
Sbjct: 15  LSQEIPATPGIRVIDIPFPLKDAFDALSWLASQ-QTYPQFYWQQRN-------------- 74

Query: 153 NGHHPFNHHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSK--KCPLIRAYGAIRFD- 212
                          G   AVV   + +F+  D    +RFL +  +   +R +G   FD 
Sbjct: 75  ---------------GDEEAVVLGAITRFTSLD--QAQRFLRQHPEHADLRIWGLNAFDP 134

Query: 213 ARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQ 272
           ++ N+            ++P++E+    G + L  T+            +  SLQ    Q
Sbjct: 135 SQGNL------------LLPRLEWRRCGGKATLRLTLF-----------SESSLQHDAIQ 194

Query: 273 VSSNIVKLQ--RDLPEVFI--LRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARST 332
               I  L   + LP + +   R  H P KT W   I  A + I  +  EL KVVLAR+T
Sbjct: 195 AKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELATKTI--AEGELDKVVLARAT 254

Query: 333 RIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAA 392
            +  +  ++  A +A  +    N Y F +      AF+GS+PERL+ R   ++R+EALA 
Sbjct: 255 DLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAG 314

Query: 393 TRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRI 452
           T A      +  ++   L+++ K+  E  +V E + ++L+A    + V P + +R L ++
Sbjct: 315 TVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLR-LRKV 374

Query: 453 QHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWF 512
           QHL   +   L ++ D+   L  L PT AV GLP + AR  IA  E F R  YAG  G+ 
Sbjct: 375 QHLRRCIWTSL-NKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYL 431

Query: 513 GGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLE 563
              QSEF V +RSA +  G    +YAG GIV+GSDP  EW E++ K + +   L +E
Sbjct: 435 SLQQSEFCVSLRSAKI-SGNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME 431

BLAST of Moc06g02000 vs. ExPASy Swiss-Prot
Match: P23973 (Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF PE=3 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 7.0e-35
Identity = 108/366 (29.51%), Postives = 173/366 (47.27%), Query Frame = 0

Query: 203 YGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVES 262
           +G   FD      S+W+ F    F VP +         ML  T          W +  E 
Sbjct: 119 FGGFSFDPCEERGSQWDHFSEGDFFVPAL---------MLTMTAEGPFLTVNRWVSGGED 178

Query: 263 LQST---IRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKV 322
            ++    ++  ++  +       +  ++       K  W  AI  A   I     +  KV
Sbjct: 179 AEAVLEGLKAFAAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQI--KEKQYDKV 238

Query: 323 VLARSTRIVTS--VDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLS 382
           VLAR   +     + I+PV  +  L  + + SY F I+      F+G++PERL  R+G +
Sbjct: 239 VLARELLLTFDGPIQIEPV--LKTLLDDQQTSYVFAIE-QEGKTFVGASPERLIKRDGGT 298

Query: 383 IRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHK 442
           + S  LA +  RG +  +D +I  +LL++ K+ LE  IV   +     + C  +      
Sbjct: 299 VMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDGP 358

Query: 443 AVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGM 502
            + K   +QHL+  + G+L+     F+++  LHPTPA+ G P E+A  +I E E   RG 
Sbjct: 359 VLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPMSRGW 418

Query: 503 YAGPVGWFGG-GQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVT 562
           YA P+GW       EFAV IRS L+E G  A ++AG GIV+ S+P SE++E ++K+  + 
Sbjct: 419 YAAPIGWIDSQDNGEFAVAIRSGLIE-GSTARLFAGCGIVEDSEPISEYEETQIKLKPMI 469

BLAST of Moc06g02000 vs. ExPASy TrEMBL
Match: A0A6J1BZ14 (Isochorismate synthase OS=Momordica charantia OX=3673 GN=LOC111007036 PE=3 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAP 360
           AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAP
Sbjct: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAP 360

Query: 361 AFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETM 420
           AFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETM
Sbjct: 361 AFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETM 420

Query: 421 KKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPT 480
           KKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPT
Sbjct: 421 KKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPT 480

Query: 481 EEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSD 540
           EEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSD
Sbjct: 481 EEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSD 540

Query: 541 PSSEWDELELKISQVTRSLNLEVAQAPKIDW 572
           PSSEWDELELKISQVTRSLNLEVAQAPKIDW
Sbjct: 541 PSSEWDELELKISQVTRSLNLEVAQAPKIDW 571

BLAST of Moc06g02000 vs. ExPASy TrEMBL
Match: A0A6J1BZK7 (Isochorismate synthase OS=Momordica charantia OX=3673 GN=LOC111007036 PE=3 SV=1)

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 571/576 (99.13%), Postives = 571/576 (99.13%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ-----VEGKNSYQFCIQ 360
           AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ     VEGKNSYQFCIQ
Sbjct: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVAYMMVEGKNSYQFCIQ 360

Query: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420
           PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI
Sbjct: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420

Query: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480
           VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV
Sbjct: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480

Query: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540
           CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI
Sbjct: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540

Query: 541 VKGSDPSSEWDELELKISQVTRSLNLEVAQAPKIDW 572
           VKGSDPSSEWDELELKISQVTRSLNLEVAQAPKIDW
Sbjct: 541 VKGSDPSSEWDELELKISQVTRSLNLEVAQAPKIDW 576

BLAST of Moc06g02000 vs. ExPASy TrEMBL
Match: A0A6J1C0W3 (isochorismate synthase 2, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007036 PE=4 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 8.3e-265
Identity = 462/467 (98.93%), Postives = 462/467 (98.93%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ-----VEGKNSYQFCIQ 360
           AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ     VEGKNSYQFCIQ
Sbjct: 301 AIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVAYMMVEGKNSYQFCIQ 360

Query: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420
           PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI
Sbjct: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420

Query: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 463
           VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE
Sbjct: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 467

BLAST of Moc06g02000 vs. ExPASy TrEMBL
Match: A0A0A0LS91 (Isochorismate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G008580 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 4.6e-239
Identity = 428/546 (78.39%), Postives = 470/546 (86.08%), Query Frame = 0

Query: 35  RCLRFRP------HGRR---RVCSESSSMDGCRG---EPEVPVGTIETRNLAAAPTAKLA 94
           RC R  P       GRR    VC +S++MDGC G   EP   VG IETRN  A  TA LA
Sbjct: 22  RCRRISPAVAWRERGRRWGGEVC-DSAAMDGCVGGSQEPGGTVGIIETRNFPAVQTAALA 81

Query: 95  MESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLA 154
            E LR GV +LKSEA PAF SGI+RLQVPIQQ+IQAIDWL SQP +LPRTFFSGRSR   
Sbjct: 82  TERLRRGVAELKSEA-PAFTSGIIRLQVPIQQRIQAIDWLSSQPHLLPRTFFSGRSRPTH 141

Query: 155 SGPLSADSIYNGHHPFN-HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIR 214
           S   SA +I NG H FN HH L+SVAGVGSAVVF RLH FSY DW SIKRFLSKKCPLIR
Sbjct: 142 S---SAHNISNGSHSFNHHHHLLSVAGVGSAVVFRRLHCFSYNDWASIKRFLSKKCPLIR 201

Query: 215 AYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVE 274
           AYG IRFD+RAN+S EWEPFGSFYFMVPQVEFDEFE NSMLAATVAWDHACSWTWENA+E
Sbjct: 202 AYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEENSMLAATVAWDHACSWTWENAIE 261

Query: 275 SLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVL 334
           SLQSTI QVSSN V LQ+DLP++ IL + HVPSKT+W+ A+ RALQ+ID SNSELTKVVL
Sbjct: 262 SLQSTIEQVSSNNVNLQKDLPQIPILSYTHVPSKTFWERAVDRALQVIDGSNSELTKVVL 321

Query: 335 ARSTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSE 394
           ARST+IVT VDIDPV WVACLQ EG NSYQFC+QPSNAPAFIG+TPERLFHREGL I+S+
Sbjct: 322 ARSTQIVTGVDIDPVTWVACLQKEGNNSYQFCVQPSNAPAFIGNTPERLFHREGLGIKSD 381

Query: 395 ALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRK 454
           ALAATRARGR+AIED+KIE+DL SNPK+DLEFSIVRET+KKKLEAVCDA++VEP+K+VRK
Sbjct: 382 ALAATRARGRTAIEDIKIEHDLFSNPKEDLEFSIVRETIKKKLEAVCDAVVVEPYKSVRK 441

Query: 455 LPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGP 514
           LPRIQHLYAQL GRL+SEEDEFEILSTLHPTPAVCGLPTEEAR  IAETE+FDRGMYAGP
Sbjct: 442 LPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEEARDFIAETEVFDRGMYAGP 501

Query: 515 VGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNL 568
           VGWFGGG+SEFAVGIRSALV++GLGALIYAGTGIVKGS+PSSEWDELELKISQ++RSL L
Sbjct: 502 VGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVKGSNPSSEWDELELKISQISRSLEL 561

BLAST of Moc06g02000 vs. ExPASy TrEMBL
Match: A0A1S3BV15 (Isochorismate synthase OS=Cucumis melo OX=3656 GN=LOC103493974 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 4.2e-232
Identity = 415/532 (78.01%), Postives = 456/532 (85.71%), Query Frame = 0

Query: 43  GRR---RVCSESSSMDGCRG----EPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKS 102
           GRR   +VC +S++MDGC G    E    VGTIETRN  A  TA LA ESLR GV +LKS
Sbjct: 44  GRRWGEKVC-DSAAMDGCGGGNNQEAGGTVGTIETRNFPAVRTAALATESLRRGVAELKS 103

Query: 103 EAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGH 162
           EA PA  SGI+RLQVPIQQ+IQAIDWL SQP +LPRTFFSGRSR   S   SA +I NG 
Sbjct: 104 EA-PALSSGIIRLQVPIQQRIQAIDWLSSQPHLLPRTFFSGRSRPTDS---SAHNISNGS 163

Query: 163 HPFNHHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVS 222
           H FNH  L+ VAGVGSAVVF RLH FSY DW SIKRFLSKKCPLIRAYG IRFD+RAN+S
Sbjct: 164 HSFNHRHLLGVAGVGSAVVFRRLHCFSYNDWASIKRFLSKKCPLIRAYGGIRFDSRANIS 223

Query: 223 SEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIV 282
            EWEPFGSFYFMVPQVEFDEFE NSMLAATVAWDHACSWTWENAVESLQSTI QVSSN V
Sbjct: 224 PEWEPFGSFYFMVPQVEFDEFEENSMLAATVAWDHACSWTWENAVESLQSTIEQVSSNNV 283

Query: 283 KLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDP 342
            LQ+D P++ IL + HVP KT+W+ A+ RALQ ID SNSELTKVVLARST+IVT VDIDP
Sbjct: 284 NLQKDPPQIPILSYTHVPGKTFWERAVDRALQEIDGSNSELTKVVLARSTQIVTGVDIDP 343

Query: 343 VAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIE 402
           V  +AC Q EG NSYQFC+QPSNA AFIG+TPERLFHREGL I+S+ALAATRARGRSAIE
Sbjct: 344 VTCIACFQKEGNNSYQFCVQPSNAHAFIGNTPERLFHREGLDIKSDALAATRARGRSAIE 403

Query: 403 DLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGR 462
           D+KIE+DL SNPK+DLEFSIVRE +KKKLEAVCDA++VEP+K+VRKLPRIQHLYAQL GR
Sbjct: 404 DIKIEHDLFSNPKEDLEFSIVREAIKKKLEAVCDAVVVEPYKSVRKLPRIQHLYAQLTGR 463

Query: 463 LKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVG 522
           L+SEEDEFEILS LHPTPAVCG PTEEAR  IAETE+FDRGMYAGPVGWFGGG+SEFAVG
Sbjct: 464 LRSEEDEFEILSMLHPTPAVCGWPTEEARDFIAETEVFDRGMYAGPVGWFGGGESEFAVG 523

Query: 523 IRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLEVAQAP 568
           IRSALV++GLGALIYAGTGIVKGS+PSSEWDELELKISQ++RSL LEV Q P
Sbjct: 524 IRSALVKRGLGALIYAGTGIVKGSNPSSEWDELELKISQISRSLELEVVQEP 570

BLAST of Moc06g02000 vs. TAIR 10
Match: AT1G18870.1 (isochorismate synthase 2 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.3e-182
Identity = 323/524 (61.64%), Postives = 404/524 (77.10%), Query Frame = 0

Query: 44  RRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGS 103
           R+R    + SM+GC  + + P+GT+ETR L+  P+   A E L   V  LKS+ PP F S
Sbjct: 41  RQRFLRCTLSMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQ-PPPFSS 100

Query: 104 GIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRL 163
           GIVRLQVPI+Q+I AIDWL +Q +ILPR+FFS RS +     L   S  NG    + H  
Sbjct: 101 GIVRLQVPIEQKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS---SDHNP 160

Query: 164 VSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGS 223
           VSVAG+GSAV F  L  FS+ DW SI+RFLS K PLIRAYG +RFD    ++ EWE FGS
Sbjct: 161 VSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGS 220

Query: 224 FYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPE 283
           FYF VPQVEFDEF G+SMLAATVAWD+  SWT ENA+E+LQ T+ QVSS I++L+R+   
Sbjct: 221 FYFTVPQVEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLG 280

Query: 284 VFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQ 343
           V ++  NHVPS+  +  A++ AL++I   +S L+KVVLARS+RI+T  DIDP+AW+A LQ
Sbjct: 281 VIVVSKNHVPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQ 340

Query: 344 VEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDL 403
            EG+++YQFC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL
Sbjct: 341 CEGQDAYQFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDL 400

Query: 404 LSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEF 463
           L++PKDDLEFSIVRE +++KL+ +CD ++V+PHK+VRKL R+QHLY+QLAG+LK E+DEF
Sbjct: 401 LTSPKDDLEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEF 460

Query: 464 EILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEK 523
            IL+ LHPTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGG+SEF+VGIRSALVEK
Sbjct: 461 NILTALHPTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEK 520

Query: 524 GLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLEVAQAP 568
           GLGALIYAGTGIV GS+PSSEW+ELELKISQ T+SL  E A  P
Sbjct: 521 GLGALIYAGTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 560

BLAST of Moc06g02000 vs. TAIR 10
Match: AT1G18870.2 (isochorismate synthase 2 )

HSP 1 Score: 634.0 bits (1634), Expect = 1.1e-181
Identity = 320/514 (62.26%), Postives = 399/514 (77.63%), Query Frame = 0

Query: 54  MDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQ 113
           M+GC  + + P+GT+ETR L+  P+   A E L   V  LKS+ PP F SGIVRLQVPI+
Sbjct: 1   MNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQ-PPPFSSGIVRLQVPIE 60

Query: 114 QQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAV 173
           Q+I AIDWL +Q +ILPR+FFS RS +     L   S  NG    + H  VSVAG+GSAV
Sbjct: 61  QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS---SDHNPVSVAGIGSAV 120

Query: 174 VFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEF 233
            F  L  FS+ DW SI+RFLS K PLIRAYG +RFD    ++ EWE FGSFYF VPQVEF
Sbjct: 121 FFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEF 180

Query: 234 DEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVP 293
           DEF G+SMLAATVAWD+  SWT ENA+E+LQ T+ QVSS I++L+R+   V ++  NHVP
Sbjct: 181 DEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVP 240

Query: 294 SKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDIDPVAWVACLQVEGKNSYQFC 353
           S+  +  A++ AL++I   +S L+KVVLARS+RI+T  DIDP+AW+A LQ EG+++YQFC
Sbjct: 241 SEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFC 300

Query: 354 IQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEF 413
           +QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DLL++PKDDLEF
Sbjct: 301 LQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEF 360

Query: 414 SIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTP 473
           SIVRE +++KL+ +CD ++V+PHK+VRKL R+QHLY+QLAG+LK E+DEF IL+ LHPTP
Sbjct: 361 SIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTP 420

Query: 474 AVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGT 533
           AVCG P EEAR LI + E FDRGMYAGP+G+FGGG+SEF+VGIRSALVEKGLGALIYAGT
Sbjct: 421 AVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGT 480

Query: 534 GIVKGSDPSSEWDELELKISQVTRSLNLEVAQAP 568
           GIV GS+PSSEW+ELELKISQ T+SL  E A  P
Sbjct: 481 GIVSGSNPSSEWNELELKISQFTKSLEHESALQP 510

BLAST of Moc06g02000 vs. TAIR 10
Match: AT1G74710.1 (ADC synthase superfamily protein )

HSP 1 Score: 616.3 bits (1588), Expect = 2.5e-176
Identity = 317/529 (59.92%), Postives = 396/529 (74.86%), Query Frame = 0

Query: 39  FRPHGRRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAP 98
           F    R++  S S SM+GC G+ + P+GT+ETR + A  +   A E L + V +LKS+ P
Sbjct: 33  FTRFSRKKYESCSMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQ-P 92

Query: 99  PAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPF 158
           P+F SG+VRLQVPI QQI AIDWL++Q +I PR FFS RS       L   +  NG+   
Sbjct: 93  PSFSSGVVRLQVPIDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNG 152

Query: 159 N-----HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARAN 218
           N        LVSVAG+GSAV F  L  FS+ DW SI+RFLS   PLIRAYG +RFD    
Sbjct: 153 NGTVSSDRNLVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGK 212

Query: 219 VSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 278
           ++ EWEPFG+FYF VPQVEF+EF G+SMLAAT+AWD   SWT ENA+E+LQ T+ QVSS 
Sbjct: 213 IAVEWEPFGAFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSV 272

Query: 279 IVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDI 338
           ++KL+     V +L  NHVP+K  +  A+ +AL+MI++ +S L KVVLAR++RI+T  DI
Sbjct: 273 VMKLRNRSLGVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDI 332

Query: 339 DPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSA 398
           DP+AW+A LQ EG ++YQFC+QP  APAFIG+TPERLF R  L + SEALAATR R  S+
Sbjct: 333 DPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASS 392

Query: 399 IEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLA 458
             D++IE DLL++PKDDLEFSIVRE +++KL  +CD ++V+P K VRKL R+QHLY+QLA
Sbjct: 393 ARDMEIERDLLTSPKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLA 452

Query: 459 GRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFA 518
           GRL  E+DE++IL+ LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG +SEFA
Sbjct: 453 GRLTKEDDEYKILAALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFA 512

Query: 519 VGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQVTRSLNLE 563
           VGIRSALVEKGLGALIYAGTGIV GSDPSSEW+EL+LKISQ T+S+  E
Sbjct: 513 VGIRSALVEKGLGALIYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560

BLAST of Moc06g02000 vs. TAIR 10
Match: AT1G74710.2 (ADC synthase superfamily protein )

HSP 1 Score: 611.7 bits (1576), Expect = 6.1e-175
Identity = 315/522 (60.34%), Postives = 392/522 (75.10%), Query Frame = 0

Query: 39  FRPHGRRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAP 98
           F    R++  S S SM+GC G+ + P+GT+ETR + A  +   A E L + V +LKS+ P
Sbjct: 33  FTRFSRKKYESCSMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQ-P 92

Query: 99  PAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPF 158
           P+F SG+VRLQVPI QQI AIDWL++Q +I PR FFS RS       L   +  NG+   
Sbjct: 93  PSFSSGVVRLQVPIDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNG 152

Query: 159 N-----HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARAN 218
           N        LVSVAG+GSAV F  L  FS+ DW SI+RFLS   PLIRAYG +RFD    
Sbjct: 153 NGTVSSDRNLVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGK 212

Query: 219 VSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 278
           ++ EWEPFG+FYF VPQVEF+EF G+SMLAAT+AWD   SWT ENA+E+LQ T+ QVSS 
Sbjct: 213 IAVEWEPFGAFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSV 272

Query: 279 IVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKVVLARSTRIVTSVDI 338
           ++KL+     V +L  NHVP+K  +  A+ +AL+MI++ +S L KVVLAR++RI+T  DI
Sbjct: 273 VMKLRNRSLGVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDI 332

Query: 339 DPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSA 398
           DP+AW+A LQ EG ++YQFC+QP  APAFIG+TPERLF R  L + SEALAATR R  S+
Sbjct: 333 DPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASS 392

Query: 399 IEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLA 458
             D++IE DLL++PKDDLEFSIVRE +++KL  +CD ++V+P K VRKL R+QHLY+QLA
Sbjct: 393 ARDMEIERDLLTSPKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLA 452

Query: 459 GRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFA 518
           GRL  E+DE++IL+ LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG +SEFA
Sbjct: 453 GRLTKEDDEYKILAALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFA 512

Query: 519 VGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQV 556
           VGIRSALVEKGLGALIYAGTGIV GSDPSSEW+EL+LKISQV
Sbjct: 513 VGIRSALVEKGLGALIYAGTGIVAGSDPSSEWNELDLKISQV 553

BLAST of Moc06g02000 vs. TAIR 10
Match: AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )

HSP 1 Score: 104.0 bits (258), Expect = 4.1e-22
Identity = 73/219 (33.33%), Postives = 112/219 (51.14%), Query Frame = 0

Query: 60  EPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAI 119
           + ++ V    TR L  A T +L +ESL+  + +LK+  PP   SG++R QV +  + +A+
Sbjct: 71  DDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTN-PPKSSSGVLRFQVAVPPRAKAL 130

Query: 120 DWLRSQP---QILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFC 179
            W  SQP    + P  FF           LS D++   +          V G+G+A  F 
Sbjct: 131 FWFCSQPTTSDVFP-VFF-----------LSKDTVEPSYKSLYVKEPHGVFGIGNAFAFV 190

Query: 180 RLHQFSYTDWTSIKRFLSKKCPLIRAYG--AIRFDARANVSSEWEPFGSFYFMVPQVEFD 239
                     + IK FLS +  ++ AYG   I F+  + V+S+    GS YF VPQ+E D
Sbjct: 191 HSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK---DGSSYFFVPQIELD 250

Query: 240 EFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 274
           E E  S+LA T+AW+ + S+T E  + S + +I QVSS+
Sbjct: 251 EHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSH 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134886.10.0e+00100.00isochorismate synthase 2, chloroplastic isoform X2 [Momordica charantia][more]
XP_022134885.10.0e+0099.13isochorismate synthase 2, chloroplastic isoform X1 [Momordica charantia][more]
XP_022134887.11.7e-26498.93isochorismate synthase 2, chloroplastic isoform X3 [Momordica charantia][more]
XP_038879640.12.4e-24281.25isochorismate synthase 2, chloroplastic isoform X1 [Benincasa hispida][more]
XP_004138153.31.2e-23879.29isochorismate synthase 2, chloroplastic isoform X1 [Cucumis sativus] >XP_0116599... [more]
Match NameE-valueIdentityDescription
Q9M9V63.2e-18161.64Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 ... [more]
Q9S7H83.5e-17559.92Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 ... [more]
Q9ZPC02.7e-17258.14Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1[more]
P380513.4e-3729.35Isochorismate synthase MenF OS=Escherichia coli (strain K12) OX=83333 GN=menF PE... [more]
P239737.0e-3529.51Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF ... [more]
Match NameE-valueIdentityDescription
A0A6J1BZ140.0e+00100.00Isochorismate synthase OS=Momordica charantia OX=3673 GN=LOC111007036 PE=3 SV=1[more]
A0A6J1BZK70.0e+0099.13Isochorismate synthase OS=Momordica charantia OX=3673 GN=LOC111007036 PE=3 SV=1[more]
A0A6J1C0W38.3e-26598.93isochorismate synthase 2, chloroplastic isoform X3 OS=Momordica charantia OX=367... [more]
A0A0A0LS914.6e-23978.39Isochorismate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G008580 PE=3 SV=1[more]
A0A1S3BV154.2e-23278.01Isochorismate synthase OS=Cucumis melo OX=3656 GN=LOC103493974 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G18870.12.3e-18261.64isochorismate synthase 2 [more]
AT1G18870.21.1e-18162.26isochorismate synthase 2 [more]
AT1G74710.12.5e-17659.92ADC synthase superfamily protein [more]
AT1G74710.26.1e-17560.34ADC synthase superfamily protein [more]
AT1G68890.14.1e-2233.33magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-su... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 260..280
NoneNo IPR availablePANTHERPTHR47253:SF4ISOCHORISMATE SYNTHASE 2, CHLOROPLASTICcoord: 48..566
IPR005801ADC synthaseGENE3D3.60.120.10Anthranilate synthasecoord: 83..561
e-value: 4.6E-105
score: 354.2
IPR005801ADC synthaseSUPERFAMILY56322ADC synthasecoord: 144..561
IPR004561Isochorismate synthaseTIGRFAMTIGR00543TIGR00543coord: 201..559
e-value: 4.2E-105
score: 349.8
IPR015890Chorismate-utilising enzyme, C-terminalPFAMPF00425Chorismate_bindcoord: 297..551
e-value: 5.7E-61
score: 206.3
IPR044250Isochorismate synthase MenF-likePANTHERPTHR47253FAMILY NOT NAMEDcoord: 48..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g02000.1Moc06g02000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042372 phylloquinone biosynthetic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0009536 plastid
molecular_function GO:0008909 isochorismate synthase activity