Moc06g00990 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g00990
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome P450
Location	chr6: 636289 .. 636840 (-)
RNA-Seq Expression	Moc06g00990
Synteny	Moc06g00990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTATTGGGGAGTTTCATTGTAAGAGACTTGATTCCGTGGCTGGGTTGGATTGATGGGATCAGTGGGATGGATGCCAAGGCAAAGAGGGTAGCCAAAGAACTTGATGATTTGATTGACAGAGTGATTCAAGATCATGTCCATCCAAATTACAACCCCGAGGAGCAGAGCAGGGACTTGGTGGACGTGCTGCTTTCAATACAGAGACAGGACTCCATCGGATTTCCCCTTGAGATGAAAAGCATAAAAGCTTTAATCCTGGTAATTTAGGTACTACAAGAAACAACCAACTAGTACACTATTACATGAAAGTTGAGACCTTTTTGTTTGGATTTTGCGTTACAGGACATGTTTGGAGGAGACACGGTATTGGAGTGGGCGATGTCAGAGCTTTTGAGTCACCCAGAAGCTATGGAGAAATTACAGGGTGAGGTGAAAGAAGTAGCAGGGGAGAAAGAATACGTGATTGAGGATGATTTAGAGAAGATGCATTACTTGAAGGCAGTGATCAAGGAAACATTGCGATTACACCACCAATCCCACTACTAG

mRNA sequence

ATGAAATTATTGGGGAGTTTCATTGTAAGAGACTTGATTCCGTGGCTGGGTTGGATTGATGGGATCAGTGGGATGGATGCCAAGGCAAAGAGGGTAGCCAAAGAACTTGATGATTTGATTGACAGAGTGATTCAAGATCATGTCCATCCAAATTACAACCCCGAGGAGCAGAGCAGGGACTTGGTGGACGTGCTGCTTTCAATACAGAGACAGGACTCCATCGGATTTCCCCTTGAGATGAAAAGCATAAAAGCTTTAATCCTGGACATGTTTGGAGGAGACACGGTATTGGAGTGGGCGATGTCAGAGCTTTTGAGTCACCCAGAAGCTATGGAGAAATTACAGGGTGAGGTGAAAGAAGTAGCAGGGGAGAAAGAATACGTGATTGAGGATGATTTAGAGAAGATGCATTACTTGAAGGCAGTGATCAAGGAAACATTGCGATTACACCACCAATCCCACTACTAG

Coding sequence (CDS)

ATGAAATTATTGGGGAGTTTCATTGTAAGAGACTTGATTCCGTGGCTGGGTTGGATTGATGGGATCAGTGGGATGGATGCCAAGGCAAAGAGGGTAGCCAAAGAACTTGATGATTTGATTGACAGAGTGATTCAAGATCATGTCCATCCAAATTACAACCCCGAGGAGCAGAGCAGGGACTTGGTGGACGTGCTGCTTTCAATACAGAGACAGGACTCCATCGGATTTCCCCTTGAGATGAAAAGCATAAAAGCTTTAATCCTGGACATGTTTGGAGGAGACACGGTATTGGAGTGGGCGATGTCAGAGCTTTTGAGTCACCCAGAAGCTATGGAGAAATTACAGGGTGAGGTGAAAGAAGTAGCAGGGGAGAAAGAATACGTGATTGAGGATGATTTAGAGAAGATGCATTACTTGAAGGCAGTGATCAAGGAAACATTGCGATTACACCACCAATCCCACTACTAG

Protein sequence

MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGDTVLEWAMSELLSHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLHHQSHY

Homology

[Close]

BLAST of Moc06g00990 vs. NCBI nr
Match: XP_022134640.1 (cytochrome P450 71A24-like [Momordica charantia])

HSP 1 Score: 258.8 bits (660), Expect = 2.9e-65
Identity = 133/156 (85.26%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRD 60
           +KLLGSF VRD +PWLGWIDGISGMDAKAK+VAKELDD +DRVIQDHVH +YNPEEQS+D
Sbjct: 214 VKLLGSFSVRDFVPWLGWIDGISGMDAKAKKVAKELDDFVDRVIQDHVHADYNPEEQSQD 273

Query: 61  LVDVLLSIQRQDSIGF-PLEMKSIKALILDMFGGD-----TVLEWAMSELLSHPEAMEKL 120
           LVDVLLSIQRQDSIGF PLEMKSIKALILDMF G      TVLEW MSELL HPEAM+KL
Sbjct: 274 LVDVLLSIQRQDSIGFPPLEMKSIKALILDMFAGGTDTIYTVLEWTMSELLRHPEAMKKL 333

Query: 121 QGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           Q EVKEVAGEKEYV EDDLEKMHYLKAVIKETLRLH
Sbjct: 334 QSEVKEVAGEKEYVSEDDLEKMHYLKAVIKETLRLH 369

[Close]

BLAST of Moc06g00990 vs. NCBI nr
Match: XP_022987235.1 (cytochrome P450 71A25-like [Cucurbita maxima])

HSP 1 Score: 209.1 bits (531), Expect = 2.6e-50
Identity = 110/157 (70.06%), Postives = 126/157 (80.25%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSR- 60
           ++LLGSF VRD IPWLGWIDGISG+DAKAKRVAKELD  +DRVIQDHV      E   + 
Sbjct: 215 LELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQDHVDSENRDENSDKH 274

Query: 61  -DLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGG-----DTVLEWAMSELLSHPEAMEK 120
            DLVDVLL IQR +SIGFPLEM SIKALILDMF        TVLEWAMSEL  HPEAM+K
Sbjct: 275 MDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLEWAMSELFKHPEAMKK 334

Query: 121 LQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           L+ E++E++G+ +YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 335 LKSEIREISGD-QYVNEDDLDKMHYLKAVMKETLRLH 370

[Close]

BLAST of Moc06g00990 vs. NCBI nr
Match: XP_023515571.1 (cytochrome P450 71A24-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.2 bits (526), Expect = 1.0e-49
Identity = 108/159 (67.92%), Postives = 125/159 (78.62%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEE--QS 60
           + LLGSF VRD IPWLGWIDGISG+DAK KRVAKELD  +DRVIQDHVH     E   + 
Sbjct: 215 LDLLGSFGVRDFIPWLGWIDGISGLDAKGKRVAKELDVFLDRVIQDHVHSENRDENSGKH 274

Query: 61  RDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGG-----DTVLEWAMSELLSHPEAMEK 120
           +DLVDVLL +QR +SIGFPLEM SIKALILDMF        TVLEW MSELL HPE M+K
Sbjct: 275 KDLVDVLLWVQRNNSIGFPLEMDSIKALILDMFAAGTETTSTVLEWTMSELLKHPEVMKK 334

Query: 121 LQGEVKEVAGEKE--YVIEDDLEKMHYLKAVIKETLRLH 151
           L+ E++E++G+ +  YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 335 LKSEIREISGDHKAYYVNEDDLDKMHYLKAVLKETLRLH 373

[Close]

BLAST of Moc06g00990 vs. NCBI nr
Match: XP_022921787.1 (cytochrome P450 71A3-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 206.1 bits (523), Expect = 2.2e-49
Identity = 109/157 (69.43%), Postives = 126/157 (80.25%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEE--QS 60
           ++LLGSF VRD IPWLGWIDGISG+DA+AKRVAKELD  + RVIQDHV      E   + 
Sbjct: 215 LELLGSFGVRDFIPWLGWIDGISGLDARAKRVAKELDVFLGRVIQDHVDSENRDENSGKH 274

Query: 61  RDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGG-----DTVLEWAMSELLSHPEAMEK 120
           +DLVDVLL IQR ++IGFPLEM SIKALILDMF        TVLEWAMSELL HPEAM+K
Sbjct: 275 KDLVDVLLWIQRNNTIGFPLEMDSIKALILDMFSAGTDTISTVLEWAMSELLKHPEAMKK 334

Query: 121 LQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           L+ E++E++G+  YV EDDL+KMHYLKAVIKETLRLH
Sbjct: 335 LKSEIREISGD-HYVNEDDLDKMHYLKAVIKETLRLH 370

[Close]

BLAST of Moc06g00990 vs. NCBI nr
Match: XP_022134746.1 (cytochrome P450 71A26-like [Momordica charantia])

HSP 1 Score: 206.1 bits (523), Expect = 2.2e-49
Identity = 110/156 (70.51%), Postives = 126/156 (80.77%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           +LLGSF  RD IPWLGWIDGISG+DAKA+RVA E+D  ++RVI+DHV       ++ +DL
Sbjct: 213 ELLGSFSGRDFIPWLGWIDGISGLDAKAERVANEIDAFLNRVIEDHVETGEISVDKHKDL 272

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMF--GGD---TVLEWAMSELLSHPEAMEKLQG 121
           VD+LLSIQ+QDSIGFPLEM SIKALILDMF  G D   TVLEWAMS+LL HPEAM+KLQ 
Sbjct: 273 VDILLSIQKQDSIGFPLEMDSIKALILDMFAAGTDTTYTVLEWAMSQLLRHPEAMKKLQT 332

Query: 122 EVKEVAG--EKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+E  G  EK  V EDDLE+MHYLKAVIKETLRLH
Sbjct: 333 EVRETVGDREKSCVSEDDLERMHYLKAVIKETLRLH 368

[Close]

BLAST of Moc06g00990 vs. ExPASy Swiss-Prot
Match: P37119 (Cytochrome P450 71A3 (Fragment) OS=Solanum melongena OX=4111 GN=CYP71A3 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.0e-37
Identity = 87/158 (55.06%), Postives = 110/158 (69.62%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEE---Q 60
           ++LLG+F V D IP L W++ I+G D+K  +VAK+LD  ++ VI+ H+  N   E    +
Sbjct: 200 VELLGTFNVGDYIPCLEWVNKITGFDSKVDKVAKDLDTFLEFVIEAHMIRNEKEENRAGE 259

Query: 61  SRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMF--GGD---TVLEWAMSELLSHPEAME 120
           S+DLVDVLL IQ     GFP++  S+KAL+LD F  G D   TVLEW M+ELL HP AME
Sbjct: 260 SKDLVDVLLEIQNGKETGFPIQRDSLKALLLDPFSAGTDTIYTVLEWTMTELLRHPRAME 319

Query: 121 KLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           KLQ EV+ +A EK  + EDDL  MHYLKAVIKETLRLH
Sbjct: 320 KLQNEVQGLAQEKAEITEDDLPNMHYLKAVIKETLRLH 357

[Close]

BLAST of Moc06g00990 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 5.9e-37
Identity = 79/155 (50.97%), Postives = 110/155 (70.97%), Query Frame = 0


Query: 3   LLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHV-HPNYNPEEQSRDL 62
           LLG+  + D +PWL W+  ++G+ AK  +VAK+LDD ID V+Q+H+ H + + ++  +D 
Sbjct: 229 LLGTVNIGDYVPWLSWLSHVNGLGAKLDKVAKQLDDFIDTVVQEHMNHSSRSGDDDQKDF 288

Query: 63  VDVLLSIQRQDSIGFPLEMKSIKALILDMF--GGDT---VLEWAMSELLSHPEAMEKLQG 122
           +D+LL+IQ++ S G P++  S+K +ILDMF  G DT    LEWAM+ELL HP  M KLQ 
Sbjct: 289 LDILLAIQKETSAGIPIDGVSVKGIILDMFAAGTDTTYSALEWAMTELLRHPRVMNKLQN 348

Query: 123 EVKEVAGEKEYVI-EDDLEKMHYLKAVIKETLRLH 151
           EV+ + G +  VI EDDL +MHYLKAV KETLRLH
Sbjct: 349 EVRGIVGNRTDVITEDDLVEMHYLKAVTKETLRLH 383

[Close]

BLAST of Moc06g00990 vs. ExPASy Swiss-Prot
Match: Q9STK9 (Cytochrome P450 71A24 OS=Arabidopsis thaliana OX=3702 GN=CYP71A24 PE=2 SV=3)

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-35
Identity = 77/154 (50.00%), Postives = 107/154 (69.48%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           +LLG F V   + WL WID I G+D +  +++ +LD+ ++RV+QDHV    + +    D 
Sbjct: 206 RLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLERVVQDHV----DGDGHKNDF 265

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMFGGD-----TVLEWAMSELLSHPEAMEKLQG 121
           VD LL+I+R+ S+GF ++  SIKA+ILD+F GD     T+LEWAM+ELL H E +++LQ 
Sbjct: 266 VDFLLTIEREKSVGFEIDRLSIKAIILDVFVGDMDTTYTLLEWAMTELLCHHECLDRLQE 325

Query: 122 EVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+ V  +K  V EDDL+ M YLKAVIKETLRLH
Sbjct: 326 EVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLH 355

[Close]

BLAST of Moc06g00990 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 148.7 bits (374), Expect = 5.6e-35
Identity = 77/156 (49.36%), Postives = 103/156 (66.03%), Query Frame = 0


Query: 3   LLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHV---HPNYNPEEQSR 62
           L+G+F V D  P   W+D ++GMDA+ KR   ELD  +D VI DH+     N +   + +
Sbjct: 211 LMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRKANGSDGVEQK 270

Query: 63  DLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGDT-----VLEWAMSELLSHPEAMEKL 122
           DLVDVLL +Q+  S+G  L   ++KA+ILDMF G T      LEWAM+EL+ HP+ MEK 
Sbjct: 271 DLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKA 330

Query: 123 QGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           Q EV+ V G+K  V E+DL ++HYLK +IKETLRLH
Sbjct: 331 QQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLH 366

[Close]

BLAST of Moc06g00990 vs. ExPASy Swiss-Prot
Match: Q9STL1 (Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 7.3e-35
Identity = 75/154 (48.70%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           KLLG F V   +PWL WID ISG+D + K+   +LD+ +++V+QDH     + + Q  D 
Sbjct: 205 KLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEKVVQDH----EDGDAQRTDF 264

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMFGGDT-----VLEWAMSELLSHPEAMEKLQG 121
           VDVLL IQR+ S+GF ++  SIKA+ILD+  G T     ++EWAM+ELL  PE + +LQ 
Sbjct: 265 VDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYALMEWAMTELLHRPECLNRLQE 324

Query: 122 EVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+ +      V EDD++ M+YLKAVIKET+RLH
Sbjct: 325 EVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLH 354

[Close]

BLAST of Moc06g00990 vs. ExPASy TrEMBL
Match: A0A6J1BYV5 (cytochrome P450 71A24-like OS=Momordica charantia OX=3673 GN=LOC111006861 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-65
Identity = 133/156 (85.26%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRD 60
           +KLLGSF VRD +PWLGWIDGISGMDAKAK+VAKELDD +DRVIQDHVH +YNPEEQS+D
Sbjct: 214 VKLLGSFSVRDFVPWLGWIDGISGMDAKAKKVAKELDDFVDRVIQDHVHADYNPEEQSQD 273

Query: 61  LVDVLLSIQRQDSIGF-PLEMKSIKALILDMFGGD-----TVLEWAMSELLSHPEAMEKL 120
           LVDVLLSIQRQDSIGF PLEMKSIKALILDMF G      TVLEW MSELL HPEAM+KL
Sbjct: 274 LVDVLLSIQRQDSIGFPPLEMKSIKALILDMFAGGTDTIYTVLEWTMSELLRHPEAMKKL 333

Query: 121 QGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           Q EVKEVAGEKEYV EDDLEKMHYLKAVIKETLRLH
Sbjct: 334 QSEVKEVAGEKEYVSEDDLEKMHYLKAVIKETLRLH 369

[Close]

BLAST of Moc06g00990 vs. ExPASy TrEMBL
Match: A0A6J1JG96 (cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.3e-50
Identity = 110/157 (70.06%), Postives = 126/157 (80.25%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSR- 60
           ++LLGSF VRD IPWLGWIDGISG+DAKAKRVAKELD  +DRVIQDHV      E   + 
Sbjct: 215 LELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQDHVDSENRDENSDKH 274

Query: 61  -DLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGG-----DTVLEWAMSELLSHPEAMEK 120
            DLVDVLL IQR +SIGFPLEM SIKALILDMF        TVLEWAMSEL  HPEAM+K
Sbjct: 275 MDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLEWAMSELFKHPEAMKK 334

Query: 121 LQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           L+ E++E++G+ +YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 335 LKSEIREISGD-QYVNEDDLDKMHYLKAVMKETLRLH 370

[Close]

BLAST of Moc06g00990 vs. ExPASy TrEMBL
Match: A0A6J1BYN7 (cytochrome P450 71A26-like OS=Momordica charantia OX=3673 GN=LOC111006942 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.1e-49
Identity = 110/156 (70.51%), Postives = 126/156 (80.77%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           +LLGSF  RD IPWLGWIDGISG+DAKA+RVA E+D  ++RVI+DHV       ++ +DL
Sbjct: 213 ELLGSFSGRDFIPWLGWIDGISGLDAKAERVANEIDAFLNRVIEDHVETGEISVDKHKDL 272

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMF--GGD---TVLEWAMSELLSHPEAMEKLQG 121
           VD+LLSIQ+QDSIGFPLEM SIKALILDMF  G D   TVLEWAMS+LL HPEAM+KLQ 
Sbjct: 273 VDILLSIQKQDSIGFPLEMDSIKALILDMFAAGTDTTYTVLEWAMSQLLRHPEAMKKLQT 332

Query: 122 EVKEVAG--EKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+E  G  EK  V EDDLE+MHYLKAVIKETLRLH
Sbjct: 333 EVRETVGDREKSCVSEDDLERMHYLKAVIKETLRLH 368

[Close]

BLAST of Moc06g00990 vs. ExPASy TrEMBL
Match: A0A6J1E1E9 (cytochrome P450 71A3-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.1e-49
Identity = 109/157 (69.43%), Postives = 126/157 (80.25%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEE--QS 60
           ++LLGSF VRD IPWLGWIDGISG+DA+AKRVAKELD  + RVIQDHV      E   + 
Sbjct: 215 LELLGSFGVRDFIPWLGWIDGISGLDARAKRVAKELDVFLGRVIQDHVDSENRDENSGKH 274

Query: 61  RDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGG-----DTVLEWAMSELLSHPEAMEK 120
           +DLVDVLL IQR ++IGFPLEM SIKALILDMF        TVLEWAMSELL HPEAM+K
Sbjct: 275 KDLVDVLLWIQRNNTIGFPLEMDSIKALILDMFSAGTDTISTVLEWAMSELLKHPEAMKK 334

Query: 121 LQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           L+ E++E++G+  YV EDDL+KMHYLKAVIKETLRLH
Sbjct: 335 LKSEIREISGD-HYVNEDDLDKMHYLKAVIKETLRLH 370

[Close]

BLAST of Moc06g00990 vs. ExPASy TrEMBL
Match: A0A6J1E4T4 (cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.8e-49
Identity = 106/159 (66.67%), Postives = 126/159 (79.25%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEE--QS 60
           ++LLGSF +RD IPWLGWIDGISG+DAK KRVAKELD  +DRVIQD VH     E   + 
Sbjct: 215 LELLGSFGIRDFIPWLGWIDGISGLDAKGKRVAKELDVFLDRVIQDRVHSENRDENSGKH 274

Query: 61  RDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGG-----DTVLEWAMSELLSHPEAMEK 120
           +DLVDVLL +QR +SIGFPLEM SIKALILDMF        TV+EW MSELL HPEAM+K
Sbjct: 275 KDLVDVLLWVQRNNSIGFPLEMDSIKALILDMFAAGTETTSTVMEWTMSELLKHPEAMKK 334

Query: 121 LQGEVKEVAGEKE--YVIEDDLEKMHYLKAVIKETLRLH 151
           L+ E++E++G+ +  YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 335 LKSEIREISGDHKAYYVNEDDLDKMHYLKAVLKETLRLH 373

[Close]

BLAST of Moc06g00990 vs. TAIR 10
Match: AT3G48290.1 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 150.6 bits (379), Expect = 1.0e-36
Identity = 77/154 (50.00%), Postives = 107/154 (69.48%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           +LLG F V   + WL WID I G+D +  +++ +LD+ ++RV+QDHV    + +    D 
Sbjct: 206 RLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLERVVQDHV----DGDGHKNDF 265

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMFGGD-----TVLEWAMSELLSHPEAMEKLQG 121
           VD LL+I+R+ S+GF ++  SIKA+ILD+F GD     T+LEWAM+ELL H E +++LQ 
Sbjct: 266 VDFLLTIEREKSVGFEIDRLSIKAIILDVFVGDMDTTYTLLEWAMTELLCHHECLDRLQE 325

Query: 122 EVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+ V  +K  V EDDL+ M YLKAVIKETLRLH
Sbjct: 326 EVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLH 355

[Close]

BLAST of Moc06g00990 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 148.3 bits (373), Expect = 5.2e-36
Identity = 75/154 (48.70%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           KLLG F V   +PWL WID ISG+D + K+   +LD+ +++V+QDH     + + Q  D 
Sbjct: 205 KLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEKVVQDH----EDGDAQRTDF 264

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMFGGDT-----VLEWAMSELLSHPEAMEKLQG 121
           VDVLL IQR+ S+GF ++  SIKA+ILD+  G T     ++EWAM+ELL  PE + +LQ 
Sbjct: 265 VDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYALMEWAMTELLHRPECLNRLQE 324

Query: 122 EVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+ +      V EDD++ M+YLKAVIKET+RLH
Sbjct: 325 EVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLH 354

[Close]

BLAST of Moc06g00990 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 145.6 bits (366), Expect = 3.3e-35
Identity = 71/154 (46.10%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           KLLG+F V   +PWL WID I G+D + ++ A ++D   +RV+QDHV  N        D 
Sbjct: 204 KLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFERVVQDHVDGN----RDMTDF 263

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMFGG-----DTVLEWAMSELLSHPEAMEKLQG 121
           VDVLL+IQR  ++GF +   SIKA+++++F G      T++EWAM+ELL HP+ +++LQ 
Sbjct: 264 VDVLLAIQRDKTVGFEINRVSIKAIVMNVFVGGTDTSSTLMEWAMTELLRHPKCLKRLQE 323

Query: 122 EVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+ +  +K  V E++++ M YLKAVIKE LRLH
Sbjct: 324 EVRTICKDKSSVSEEEIQNMSYLKAVIKEALRLH 353

[Close]

BLAST of Moc06g00990 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 143.7 bits (361), Expect = 1.3e-34
Identity = 72/154 (46.75%), Postives = 101/154 (65.58%), Query Frame = 0


Query: 2   KLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDL 61
           +LLG+F +   +PWL W+D I G DA+  ++ K+LDD  ++V+QDH            DL
Sbjct: 203 ELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDDFFEKVVQDH---EDGDRRDGTDL 262

Query: 62  VDVLLSIQRQDSIGFPLEMKSIKALILDMFGGD-----TVLEWAMSELLSHPEAMEKLQG 121
           +D LL ++R+ S GF +E  SIKA+ LD+F G      T+LEWAM+ELL HP+++ +LQ 
Sbjct: 263 IDALLRVKREKSPGFEIERVSIKAITLDVFVGGSDTSFTLLEWAMTELLRHPKSLNRLQE 322

Query: 122 EVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
           EV+ +   K  V EDD++ M YLKAVIKE LRLH
Sbjct: 323 EVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLH 353

[Close]

BLAST of Moc06g00990 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 141.7 bits (356), Expect = 4.8e-34
Identity = 72/155 (46.45%), Postives = 101/155 (65.16%), Query Frame = 0


Query: 1   MKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRD 60
           M L+G F V   +PWLGWID ISG+D +  +   +LD+ +++V+QDHV    + + Q  D
Sbjct: 204 MMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDEFLEKVVQDHV----DGDGQRTD 263

Query: 61  LVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGDT-----VLEWAMSELLSHPEAMEKLQ 120
            VDVLL IQR+ SIGF ++   IKA++LD+    T     +++WAM+ELL HPE +  LQ
Sbjct: 264 FVDVLLRIQREKSIGFEIDRLCIKAIVLDVLVAGTDSSYALMDWAMTELLRHPECLRTLQ 323

Query: 121 GEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 151
            EV+ +      V E+D++ M YLKAVIKET RLH
Sbjct: 324 EEVRTICKGNLSVSEEDIQNMSYLKAVIKETTRLH 354

The following BLAST results are available for this feature:

BLAST of Moc06g00990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134640.1	2.9e-65	85.26	cytochrome P450 71A24-like [Momordica charantia]	[more]
XP_022987235.1	2.6e-50	70.06	cytochrome P450 71A25-like [Cucurbita maxima]	[more]
XP_023515571.1	1.0e-49	67.92	cytochrome P450 71A24-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022921787.1	2.2e-49	69.43	cytochrome P450 71A3-like isoform X4 [Cucurbita moschata]	[more]
XP_022134746.1	2.2e-49	70.51	cytochrome P450 71A26-like [Momordica charantia]	[more]

BLAST of Moc06g00990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P37119	2.0e-37	55.06	Cytochrome P450 71A3 (Fragment) OS=Solanum melongena OX=4111 GN=CYP71A3 PE=2 SV=...	[more]
A0A068Q6L2	5.9e-37	50.97	Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1	[more]
Q9STK9	1.5e-35	50.00	Cytochrome P450 71A24 OS=Arabidopsis thaliana OX=3702 GN=CYP71A24 PE=2 SV=3	[more]
P24465	5.6e-35	49.36	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
Q9STL1	7.3e-35	48.70	Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1	[more]

BLAST of Moc06g00990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYV5	1.4e-65	85.26	cytochrome P450 71A24-like OS=Momordica charantia OX=3673 GN=LOC111006861 PE=3 S...	[more]
A0A6J1JG96	1.3e-50	70.06	cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1	[more]
A0A6J1BYN7	1.1e-49	70.51	cytochrome P450 71A26-like OS=Momordica charantia OX=3673 GN=LOC111006942 PE=3 S...	[more]
A0A6J1E1E9	1.1e-49	69.43	cytochrome P450 71A3-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC1114299...	[more]
A0A6J1E4T4	1.8e-49	66.67	cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429...	[more]

BLAST of Moc06g00990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48290.1	1.0e-36	50.00	cytochrome P450, family 71, subfamily A, polypeptide 24	[more]
AT3G48310.1	5.2e-36	48.70	cytochrome P450, family 71, subfamily A, polypeptide 22	[more]
AT3G48270.1	3.3e-35	46.10	cytochrome P450, family 71, subfamily A, polypeptide 26	[more]
AT3G48280.1	1.3e-34	46.75	cytochrome P450, family 71, subfamily A, polypeptide 25	[more]
AT3G48320.1	4.8e-34	46.45	cytochrome P450, family 71, subfamily A, polypeptide 21	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 97..123 score: 36.53 coord: 140..155 score: 27.84
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..154 e-value: 1.3E-29 score: 105.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..151
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 24..153 e-value: 3.0E-25 score: 88.9
None	No IPR available	PANTHER	PTHR47955	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 1..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g00990.1	Moc06g00990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen