Moc05g34060 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g34060
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPhenylalanyl-tRNA synthetase
Locationchr5: 25367695 .. 25370379 (+)
RNA-Seq ExpressionMoc05g34060
SyntenyMoc05g34060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTCTTTTGAGCATGGATTACCAAGATCCTAACGTGCTACTAGACTTTGATACCAAATGTAACGCTCCAGGATTTTCAACTTTCCAAACGAGGACTAAGTAAGAAAACCACCACGCTTCCACTGCACTACTCTGATTTAAACTCCACCATTTCCATATGTTACCTACTTAAGAGGTAGTCTGTGCAATTTGCCCAATATATATAAATATATGTTTAGTTGATTTATTTCTATTTTGAAATGTCTTGTAGGCCATTTGTCTCAGATATTTGCATCCGGATACTGGACTTCAGGACTTTGCTCTGCAGGTTATGGACATTCTTCATGCTGATACTGCCGTGGATGCCCATTCGCTGGTAAATGCTGGAATTTCTTTTTTCTTTTTTTAATAAACTATCTATAATTATTACTTTAGGTTGTTAAGCCTTGGCACTTGGCTGCATCGCTTGCCCAGGTGGAAATATTCTCTTGTCCAAGCTTCCTTCTTTGATTAGGGACATCAATAATTTATTAAAGAAGTCAATATACTGCTCTAACAAGGATAGCAAAAATTTGAGGTCTCACAGTGCTAACCATAGGAAAAATAAAATGCACCAGGAGTTGAGCAACACCGGAGAAAATTAGATTTATCTTCTCAATTAATTGTTCAATGGAACAATTTATAAATCAGCATGAAGTCACCCTCAGTTCCTAAGGATTTATCTTAATTTCCTAGATTGTACCAACCTCACTTCCCAAGGATTTCAAAATTTCTTTGAGTTTGAAAAACAGAAGAATGAGAATCTATAAAACACGAACACGGCAAAAAAGGAGGAGTGTCTGTGTCGGACACGGATACGTCCTGACACGCTCCGGACACGTGTCCGACACGCAATTTAAAGTGTCCGAATATTAAATTTTTTATTTTAATTTCAGACACGTGGGGACACGTTTTGGACACGGGAGGGACATGCCAGATAAAAAAATATTGTTTTTTTAGTCCAAGAAGTTTGGCTGAAACCTCAAAAACTTAAACTACAAAAAAAAATCCCTAAAACGCTACCTAGTGACCCACCCTTCTGTTTGTGTGAGTTAAATTAAATAAGCTCTATTCTTACTTCCTTTTTTCCCTAATTTCATAGGAATTAAACTGGAGTAGAATATGTTTTACTAAATTAGGATAGGATGATTATAGACAGTTAGATATATGAGGGAGAATTTTAAAAATTGTATTTCAAACCTATATAAAATCAACTTGCATCCAGCTAGACTATGATGGGAATGGAGAATATAAGGATTTGACATTACTAAGCTGTTGAAGTGTTGACCGAAAATTCAAACGGATGAACAATATGAATCAAGGGATTAGGGGTCACTTTGCTTGATTTTTGGCCAAATTTTCAAAACCTGAGTTTTCTATACTTTTGCTTATCTCAGCAATCTCAACAAGAATGCCAAAAGACACGAATTGCCATTCTCTCCCTCGATAAATATGAAAATCAATTTTAGTTTCCTTTATCTCTCTCCTCTCTCTCTCTCTTTTTAATCTTTTTCATAAAATATGTGATTCTTAATTCCATACTTCCTCTCTCTCCTCTATCCAAAAAATTTTTTTTTCCTCACAAACTTCGTAGGTGACTGGTGGCAATTTTACACAGTGACATCAGTCCACTCGCCAATGGAGGTTTGCTTATGAAGTCACTGCTTTTTATTATTTTTTTTTTAATTTTATGAGATGAGTTGCAATAAGATTTCTTTTGAAAATACAGAGTGAAGATATGATAGAGATCCAAACAAAATTAGAAGTTTTAGAATTTTAATACCATAGGGAGCTTCCTTAAAAAGGAAAAAAACCTGGGGTTCGTAGGGTTTTGGGGTTCATAGGGGGTGGGGGGTTGGGGTTTGGCGTTTTGGGCTTTGGGGTTGGGGGCTTACGGTTTGGGGTTGGGGTTGGGGTTTAGGTTTTTTCTAGAATGAGCACTAAATCTGGAAGAGTAAGTCTCTCTTTCCAAGAAGGAAAGATAACTTTTCTCTAGAACTAGATCTAGAATTGGGCTCATAATTCGAACGAGGTCTTGTAGGAAAAAATTTTCTTTTATGCTTTACATGTTGTATTATTATTATTATTCTTATTATTTTTTTTTTTAAAAAGTGGGCTCAGTTTGGTAGTTTGTCATCTTTCGAGGCAATTGTAAGTTATTTTTCCTAAATTACAAAAGCTCACGTGTTGTTTGAAGTATTGAATTTTATACTATGCCTTTTGTATCTTAATCCTATGATTATGTCTGCAGAATTTTGATGATGTGTTGGTTCCAGCAGATCATGTAAGTCAAAGTTATAATGATAGATACTATATTGACTCGCAAACTGTTTTGAGATGTCATACAAGTGCTCATCAAGCGGAGCTGTTGAGAAGAGGAAATACTCAATTTCTTGTGACAGGAGATGTGTACCGTCAGGATTCCATTGACTCAACTCATTATCCTGTATTCTATCAGGTAATCTAAATGAATATAGTAATCAAGTCTTCTGATGAATCTACCAAAGTTCTCATTTGATGAATTTATTTGCATACTTCTTAGATGGAAGGTGTCTGTGTTATTTCTCTAGATGATTGGGAGGCATCTGGGATGGATAGAACATCTTATGCAGCTGATGACTTGGAAGGTTTGGCAAGGCATTTATTTGGTAAGATTTGTGTATGA

mRNA sequence

ATGGTTCTTTTGAGCATGGATTACCAAGATCCTAACGTGCTACTAGACTTTGATACCAAATGTAACGCTCCAGGATTTTCAACTTTCCAAACGAGGACTAAATATTTGCATCCGGATACTGGACTTCAGGACTTTGCTCTGCAGGTTATGGACATTCTTCATGCTGATACTGCCGTGGATGCCCATTCGCTGAATTTTGATGATGTGTTGGTTCCAGCAGATCATGTAAGTCAAAGTTATAATGATAGATACTATATTGACTCGCAAACTGTTTTGAGATGTCATACAAGTGCTCATCAAGCGGAGCTGTTGAGAAGAGGAAATACTCAATTTCTTGTGACAGGAGATGTGTACCGTCAGGATTCCATTGACTCAACTCATTATCCTGTATTCTATCAGATGGAAGGTGTCTGTGTTATTTCTCTAGATGATTGGGAGGCATCTGGGATGGATAGAACATCTTATGCAGCTGATGACTTGGAAGGTTTGGCAAGGCATTTATTTGGTAAGATTTGTGTATGA

Coding sequence (CDS)

ATGGTTCTTTTGAGCATGGATTACCAAGATCCTAACGTGCTACTAGACTTTGATACCAAATGTAACGCTCCAGGATTTTCAACTTTCCAAACGAGGACTAAATATTTGCATCCGGATACTGGACTTCAGGACTTTGCTCTGCAGGTTATGGACATTCTTCATGCTGATACTGCCGTGGATGCCCATTCGCTGAATTTTGATGATGTGTTGGTTCCAGCAGATCATGTAAGTCAAAGTTATAATGATAGATACTATATTGACTCGCAAACTGTTTTGAGATGTCATACAAGTGCTCATCAAGCGGAGCTGTTGAGAAGAGGAAATACTCAATTTCTTGTGACAGGAGATGTGTACCGTCAGGATTCCATTGACTCAACTCATTATCCTGTATTCTATCAGATGGAAGGTGTCTGTGTTATTTCTCTAGATGATTGGGAGGCATCTGGGATGGATAGAACATCTTATGCAGCTGATGACTTGGAAGGTTTGGCAAGGCATTTATTTGGTAAGATTTGTGTATGA

Protein sequence

MVLLSMDYQDPNVLLDFDTKCNAPGFSTFQTRTKYLHPDTGLQDFALQVMDILHADTAVDAHSLNFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSIDSTHYPVFYQMEGVCVISLDDWEASGMDRTSYAADDLEGLARHLFGKICV
Homology
BLAST of Moc05g34060 vs. NCBI nr
Match: XP_022146633.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >XP_022146634.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 196.8 bits (499), Expect = 1.5e-46
Identity = 95/111 (85.59%), Postives = 102/111 (91.89%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+SYND YYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYR+DSID
Sbjct: 128 NFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRRDSID 187

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEGV V+S+DDWEASG D TSYAA+D    LEGLARHLFG +
Sbjct: 188 STHYPVFHQMEGVRVVSVDDWEASGTDGTSYAANDLKKCLEGLARHLFGAV 238

BLAST of Moc05g34060 vs. NCBI nr
Match: XP_023544878.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 191.8 bits (486), Expect = 4.9e-45
Identity = 93/113 (82.30%), Postives = 101/113 (89.38%), Query Frame = 0

Query: 63  SLNFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDS 122
           ++NFDDVLVP DHVS+SYND YYIDSQTVLRCHTSAHQAELLRRG TQFLVTGDVYR+DS
Sbjct: 47  NMNFDDVLVPVDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDS 106

Query: 123 IDSTHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           IDSTHYPVF+QMEGV V SL+DWEASG D TS+AADD    LEGLARHLFG +
Sbjct: 107 IDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAV 159

BLAST of Moc05g34060 vs. NCBI nr
Match: XP_022926060.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 191.4 bits (485), Expect = 6.4e-45
Identity = 93/113 (82.30%), Postives = 101/113 (89.38%), Query Frame = 0

Query: 63  SLNFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDS 122
           ++NFDDVLVP DHVS+SYND YYIDSQTVLRCHTSAHQAELLRRG TQFLVTGDVYR+DS
Sbjct: 47  NMNFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDS 106

Query: 123 IDSTHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           IDSTHYPVF+QMEGV V SL+DWEASG D TS+AADD    LEGLARHLFG +
Sbjct: 107 IDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAV 159

BLAST of Moc05g34060 vs. NCBI nr
Match: KAE7995906.1 (hypothetical protein FH972_000667 [Carpinus fangiana])

HSP 1 Score: 190.7 bits (483), Expect = 1.1e-44
Identity = 92/111 (82.88%), Postives = 100/111 (90.09%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+SYND YYIDSQTVLRCHTSAHQAELLR+G+T FLVTGDVYR+DSID
Sbjct: 134 NFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRKGHTHFLVTGDVYRRDSID 193

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEGV V S DDWEASG+D TSYAA+D    LEGLARHLFG +
Sbjct: 194 STHYPVFHQMEGVRVFSPDDWEASGIDSTSYAAEDLKKCLEGLARHLFGAV 244

BLAST of Moc05g34060 vs. NCBI nr
Match: KAE7995907.1 (hypothetical protein FH972_000667 [Carpinus fangiana])

HSP 1 Score: 190.7 bits (483), Expect = 1.1e-44
Identity = 92/111 (82.88%), Postives = 100/111 (90.09%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+SYND YYIDSQTVLRCHTSAHQAELLR+G+T FLVTGDVYR+DSID
Sbjct: 134 NFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRKGHTHFLVTGDVYRRDSID 193

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEGV V S DDWEASG+D TSYAA+D    LEGLARHLFG +
Sbjct: 194 STHYPVFHQMEGVRVFSPDDWEASGIDSTSYAAEDLKKCLEGLARHLFGAV 244

BLAST of Moc05g34060 vs. ExPASy Swiss-Prot
Match: Q94K73 (Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g58140 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.1e-44
Identity = 85/111 (76.58%), Postives = 96/111 (86.49%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+S ND YY+DSQTVLRCHTSAHQAELLR+G+++FLVTGDVYR+DSID
Sbjct: 131 NFDDVLVPADHVSRSLNDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLVTGDVYRRDSID 190

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEG CV S +DW  SG D T YAA+D    LEGLARHLFG +
Sbjct: 191 STHYPVFHQMEGFCVFSPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFGSV 241

BLAST of Moc05g34060 vs. ExPASy Swiss-Prot
Match: Q86A68 (Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=mpheS PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.0e-21
Identity = 47/75 (62.67%), Postives = 57/75 (76.00%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFD++L P DHV +S ND YY     +LR HTSAHQ++LLR     FLVTGDVYR+D+ID
Sbjct: 109 NFDELLFPVDHVGRSPNDTYYFSKDQLLRTHTSAHQSQLLREQEKAFLVTGDVYRRDTID 168

Query: 125 STHYPVFYQMEGVCV 140
           + HYPVF+QMEGV V
Sbjct: 169 AVHYPVFHQMEGVKV 183

BLAST of Moc05g34060 vs. ExPASy Swiss-Prot
Match: O16129 (Probable phenylalanine--tRNA ligase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=PheRS-m PE=2 SV=3)

HSP 1 Score: 100.9 bits (250), Expect = 1.5e-20
Identity = 45/79 (56.96%), Postives = 61/79 (77.22%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFD++L+PADHVS+  +D YYI+ Q +LR HT+AHQ EL+  G   FLV G+VYR+D ID
Sbjct: 110 NFDNLLIPADHVSRQKSDCYYINQQHLLRAHTTAHQVELISGGLDNFLVVGEVYRRDEID 169

Query: 125 STHYPVFYQMEGVCVISLD 144
           STHYPVF+Q + V +++ D
Sbjct: 170 STHYPVFHQADAVRLVTKD 188

BLAST of Moc05g34060 vs. ExPASy Swiss-Prot
Match: Q6AYQ3 (Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fars2 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 7.4e-20
Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFD +L+PADH S+   D YY++   +LR HTSAHQ +LL  G   FLV GDVYR+D ID
Sbjct: 125 NFDSLLIPADHPSRKKGDNYYLNRGHMLRAHTSAHQWDLLHAGLNAFLVVGDVYRRDQID 184

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTS 155
           S HYPVF+Q+EGV + S  +  A   D  S
Sbjct: 185 SQHYPVFHQLEGVRLFSKHELFAGVKDGES 214

BLAST of Moc05g34060 vs. ExPASy Swiss-Prot
Match: Q99M01 (Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Fars2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.1e-19
Identity = 47/90 (52.22%), Postives = 59/90 (65.56%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFD +L+PADH S+   D YY++   +LR HTSAHQ +LL  G   FLV GDVYR+D ID
Sbjct: 125 NFDSLLIPADHPSRKKGDNYYLNRAHMLRAHTSAHQWDLLHAGLNAFLVVGDVYRRDQID 184

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTS 155
             HYPVF+Q+EGV + S  +  A   D  S
Sbjct: 185 CQHYPVFHQLEGVRLFSKHELFAGVKDGES 214

BLAST of Moc05g34060 vs. ExPASy TrEMBL
Match: A0A6J1CZ26 (Phenylalanyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015788 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 7.3e-47
Identity = 95/111 (85.59%), Postives = 102/111 (91.89%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+SYND YYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYR+DSID
Sbjct: 128 NFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRRDSID 187

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEGV V+S+DDWEASG D TSYAA+D    LEGLARHLFG +
Sbjct: 188 STHYPVFHQMEGVRVVSVDDWEASGTDGTSYAANDLKKCLEGLARHLFGAV 238

BLAST of Moc05g34060 vs. ExPASy TrEMBL
Match: A0A6J1EJY2 (Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433292 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.1e-45
Identity = 93/113 (82.30%), Postives = 101/113 (89.38%), Query Frame = 0

Query: 63  SLNFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDS 122
           ++NFDDVLVP DHVS+SYND YYIDSQTVLRCHTSAHQAELLRRG TQFLVTGDVYR+DS
Sbjct: 47  NMNFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDS 106

Query: 123 IDSTHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           IDSTHYPVF+QMEGV V SL+DWEASG D TS+AADD    LEGLARHLFG +
Sbjct: 107 IDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAV 159

BLAST of Moc05g34060 vs. ExPASy TrEMBL
Match: A0A5N6Q9J1 (Phenylalanine--tRNA ligase OS=Carpinus fangiana OX=176857 GN=FH972_000667 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.3e-45
Identity = 92/111 (82.88%), Postives = 100/111 (90.09%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+SYND YYIDSQTVLRCHTSAHQAELLR+G+T FLVTGDVYR+DSID
Sbjct: 134 NFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRKGHTHFLVTGDVYRRDSID 193

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEGV V S DDWEASG+D TSYAA+D    LEGLARHLFG +
Sbjct: 194 STHYPVFHQMEGVRVFSPDDWEASGIDSTSYAAEDLKKCLEGLARHLFGAV 244

BLAST of Moc05g34060 vs. ExPASy TrEMBL
Match: A0A5N6Q9P5 (Phenylalanyl-tRNA synthetase OS=Carpinus fangiana OX=176857 GN=FH972_000667 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.3e-45
Identity = 92/111 (82.88%), Postives = 100/111 (90.09%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+SYND YYIDSQTVLRCHTSAHQAELLR+G+T FLVTGDVYR+DSID
Sbjct: 134 NFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRKGHTHFLVTGDVYRRDSID 193

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEGV V S DDWEASG+D TSYAA+D    LEGLARHLFG +
Sbjct: 194 STHYPVFHQMEGVRVFSPDDWEASGIDSTSYAAEDLKKCLEGLARHLFGAV 244

BLAST of Moc05g34060 vs. ExPASy TrEMBL
Match: A0A6J1EDY7 (Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433292 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 6.9e-45
Identity = 93/111 (83.78%), Postives = 99/111 (89.19%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVP DHVS+SYND YYIDSQTVLRCHTSAHQAELLRRG TQFLVTGDVYR+DSID
Sbjct: 127 NFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSID 186

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEGV V SL+DWEASG D TS+AADD    LEGLARHLFG +
Sbjct: 187 STHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAV 237

BLAST of Moc05g34060 vs. TAIR 10
Match: AT3G58140.1 (phenylalanyl-tRNA synthetase class IIc family protein )

HSP 1 Score: 181.0 bits (458), Expect = 8.0e-46
Identity = 85/111 (76.58%), Postives = 96/111 (86.49%), Query Frame = 0

Query: 65  NFDDVLVPADHVSQSYNDRYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDVYRQDSID 124
           NFDDVLVPADHVS+S ND YY+DSQTVLRCHTSAHQAELLR+G+++FLVTGDVYR+DSID
Sbjct: 131 NFDDVLVPADHVSRSLNDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLVTGDVYRRDSID 190

Query: 125 STHYPVFYQMEGVCVISLDDWEASGMDRTSYAADD----LEGLARHLFGKI 172
           STHYPVF+QMEG CV S +DW  SG D T YAA+D    LEGLARHLFG +
Sbjct: 191 STHYPVFHQMEGFCVFSPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFGSV 241

BLAST of Moc05g34060 vs. TAIR 10
Match: AT1G67140.1 (HEAT repeat-containing protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.6e-04
Identity = 16/33 (48.48%), Postives = 26/33 (78.79%), Query Frame = 0

Query: 32  RTKYLHPDTGLQDFALQVMDILHADTAVDAHSL 65
           R +YL  D+ LQD++L +MD+L  D+++DAH+L
Sbjct: 323 RIRYLDSDSELQDYSLPIMDMLRGDSSIDAHAL 355

BLAST of Moc05g34060 vs. TAIR 10
Match: AT1G67140.3 (HEAT repeat-containing protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.6e-04
Identity = 16/33 (48.48%), Postives = 26/33 (78.79%), Query Frame = 0

Query: 32  RTKYLHPDTGLQDFALQVMDILHADTAVDAHSL 65
           R +YL  D+ LQD++L +MD+L  D+++DAH+L
Sbjct: 323 RIRYLDSDSELQDYSLPIMDMLRGDSSIDAHAL 355

BLAST of Moc05g34060 vs. TAIR 10
Match: AT1G67140.2 (HEAT repeat-containing protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.6e-04
Identity = 16/33 (48.48%), Postives = 26/33 (78.79%), Query Frame = 0

Query: 32  RTKYLHPDTGLQDFALQVMDILHADTAVDAHSL 65
           R +YL  D+ LQD++L +MD+L  D+++DAH+L
Sbjct: 323 RIRYLDSDSELQDYSLPIMDMLRGDSSIDAHAL 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146633.11.5e-4685.59phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >X... [more]
XP_023544878.14.9e-4582.30phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Cucurbi... [more]
XP_022926060.16.4e-4582.30phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Cucurbi... [more]
KAE7995906.11.1e-4482.88hypothetical protein FH972_000667 [Carpinus fangiana][more]
KAE7995907.11.1e-4482.88hypothetical protein FH972_000667 [Carpinus fangiana][more]
Match NameE-valueIdentityDescription
Q94K731.1e-4476.58Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana ... [more]
Q86A683.0e-2162.67Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum OX=44689 G... [more]
O161291.5e-2056.96Probable phenylalanine--tRNA ligase, mitochondrial OS=Drosophila melanogaster OX... [more]
Q6AYQ37.4e-2053.33Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fars2... [more]
Q99M012.1e-1952.22Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Fars2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1CZ267.3e-4785.59Phenylalanyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015788 PE=3... [more]
A0A6J1EJY23.1e-4582.30Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433292 PE=3 ... [more]
A0A5N6Q9J15.3e-4582.88Phenylalanine--tRNA ligase OS=Carpinus fangiana OX=176857 GN=FH972_000667 PE=4 S... [more]
A0A5N6Q9P55.3e-4582.88Phenylalanyl-tRNA synthetase OS=Carpinus fangiana OX=176857 GN=FH972_000667 PE=3... [more]
A0A6J1EDY76.9e-4583.78Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433292 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G58140.18.0e-4676.58phenylalanyl-tRNA synthetase class IIc family protein [more]
AT1G67140.17.6e-0448.48HEAT repeat-containing protein [more]
AT1G67140.37.6e-0448.48HEAT repeat-containing protein [more]
AT1G67140.27.6e-0448.48HEAT repeat-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002319Phenylalanyl-tRNA synthetasePFAMPF01409tRNA-synt_2dcoord: 55..139
e-value: 1.2E-16
score: 60.9
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 25..167
e-value: 6.6E-35
score: 122.8
NoneNo IPR availablePANTHERPTHR11538PHENYLALANYL-TRNA SYNTHETASEcoord: 57..171
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 63..141
IPR004530Phenylalanyl-tRNA synthetase, class IIc, mitochondrialPANTHERPTHR11538:SF41PHENYLALANINE--TRNA LIGASE, MITOCHONDRIALcoord: 57..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g34060.1Moc05g34060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006432 phenylalanyl-tRNA aminoacylation
biological_process GO:0043039 tRNA aminoacylation
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004826 phenylalanine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding