Moc05g32170 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g32170
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RING-type E3 ubiquitin transferase
Location	chr5: 24082636 .. 24083175 (+)
RNA-Seq Expression	Moc05g32170
Synteny	Moc05g32170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAGCAGCACCGCCGTGATGAATCGACCAACGCCACCGTTCCCTTCGCCGCATCGGAGCGTGGACTTCTCTCCGATCGAGTTCATCCTCGGCCTCGTTGCCGTCATCACTATCCCTGCCCTAGTTTACTCCTTCTTCTTCGCAATCAAGTGCCCGCCGAACCTCCGCCTCTGGCGACGGAGATCCGCTGGCGCCGGCGCCGGCGCCGTGGCGGCCGGAAATCGAACTTTTTCTAGTGACGTGGCGTTGGCCGTTAAGTACAAGAAGGAGGAGCACTGTAAGGAAGTCGGCAGCGAGTGCCCGGTGTGCCTGTCGGCGTTCGGCGACGGCGAGGAGGTCCGGCAGCTCACCGCCTGTAAGCACTCTTTTCATGTGGCTTGCATCGACATGTGGCTCTGTTCTCACCCTAATTGCCCTGTTTGCCGCGCCTCCATCGCCGTCGCCGTTAAACGGCCGATTAGCCACCGCGCGCCGGTGCCGGCGCCTAGAAACGACGATTTCCAGCAAGGTTTGCCCGATGCCGGGACTTTAGTTTGA

mRNA sequence

ATGGACAGCAGCACCGCCGTGATGAATCGACCAACGCCACCGTTCCCTTCGCCGCATCGGAGCGTGGACTTCTCTCCGATCGAGTTCATCCTCGGCCTCGTTGCCGTCATCACTATCCCTGCCCTAGTTTACTCCTTCTTCTTCGCAATCAAGTGCCCGCCGAACCTCCGCCTCTGGCGACGGAGATCCGCTGGCGCCGGCGCCGGCGCCGTGGCGGCCGGAAATCGAACTTTTTCTAGTGACGTGGCGTTGGCCGTTAAGTACAAGAAGGAGGAGCACTGTAAGGAAGTCGGCAGCGAGTGCCCGGTGTGCCTGTCGGCGTTCGGCGACGGCGAGGAGGTCCGGCAGCTCACCGCCTGTAAGCACTCTTTTCATGTGGCTTGCATCGACATGTGGCTCTGTTCTCACCCTAATTGCCCTGTTTGCCGCGCCTCCATCGCCGTCGCCGTTAAACGGCCGATTAGCCACCGCGCGCCGGTGCCGGCGCCTAGAAACGACGATTTCCAGCAAGGTTTGCCCGATGCCGGGACTTTAGTTTGA

Coding sequence (CDS)

ATGGACAGCAGCACCGCCGTGATGAATCGACCAACGCCACCGTTCCCTTCGCCGCATCGGAGCGTGGACTTCTCTCCGATCGAGTTCATCCTCGGCCTCGTTGCCGTCATCACTATCCCTGCCCTAGTTTACTCCTTCTTCTTCGCAATCAAGTGCCCGCCGAACCTCCGCCTCTGGCGACGGAGATCCGCTGGCGCCGGCGCCGGCGCCGTGGCGGCCGGAAATCGAACTTTTTCTAGTGACGTGGCGTTGGCCGTTAAGTACAAGAAGGAGGAGCACTGTAAGGAAGTCGGCAGCGAGTGCCCGGTGTGCCTGTCGGCGTTCGGCGACGGCGAGGAGGTCCGGCAGCTCACCGCCTGTAAGCACTCTTTTCATGTGGCTTGCATCGACATGTGGCTCTGTTCTCACCCTAATTGCCCTGTTTGCCGCGCCTCCATCGCCGTCGCCGTTAAACGGCCGATTAGCCACCGCGCGCCGGTGCCGGCGCCTAGAAACGACGATTTCCAGCAAGGTTTGCCCGATGCCGGGACTTTAGTTTGA

Protein sequence

MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWRRRSAGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQGLPDAGTLV

Homology

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BLAST of Moc05g32170 vs. NCBI nr
Match: XP_022145690.1 (RING-H2 finger protein ATL33 [Momordica charantia])

HSP 1 Score: 372.1 bits (954), Expect = 2.7e-99
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWR 60
           MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWR
Sbjct: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWR 60

Query: 61  RRSAGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQLTAC 120
           RRSAGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQLTAC
Sbjct: 61  RRSAGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQLTAC 120

Query: 121 KHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQGLPDAGTLV 180
           KHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQGLPDAGTLV
Sbjct: 121 KHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQGLPDAGTLV 179

[Close]

BLAST of Moc05g32170 vs. NCBI nr
Match: KAE8651013.1 (hypothetical protein Csa_001104 [Cucumis sativus])

HSP 1 Score: 274.2 bits (700), Expect = 7.7e-70
Identity = 138/185 (74.59%), Postives = 153/185 (82.70%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD++ + MNR  PP+P+PHR+VDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101

Query: 61  RRRS---AGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQ 120
           R+RS   +GAGA  V  GNRT SSDVA AVKY K+EHCKEVG+ECPVCLSAF DGEE+RQ
Sbjct: 102 RQRSTRLSGAGA-TVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQ 161

Query: 121 LTACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPD 180
           L  CKHSFHV CIDMWL SHPNCPVCRA++ V VKRPI + AP    A R+DDF QGLPD
Sbjct: 162 LAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPD 221

[Close]

BLAST of Moc05g32170 vs. NCBI nr
Match: XP_004137943.1 (RING-H2 finger protein ATL33 [Cucumis sativus])

HSP 1 Score: 274.2 bits (700), Expect = 7.7e-70
Identity = 138/185 (74.59%), Postives = 153/185 (82.70%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD++ + MNR  PP+P+PHR+VDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 61  RRRS---AGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQ 120
           R+RS   +GAGA  V  GNRT SSDVA AVKY K+EHCKEVG+ECPVCLSAF DGEE+RQ
Sbjct: 61  RQRSTRLSGAGA-TVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQ 120

Query: 121 LTACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPD 180
           L  CKHSFHV CIDMWL SHPNCPVCRA++ V VKRPI + AP    A R+DDF QGLPD
Sbjct: 121 LAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPD 180

[Close]

BLAST of Moc05g32170 vs. NCBI nr
Match: XP_038905078.1 (RING-H2 finger protein ATL33 [Benincasa hispida])

HSP 1 Score: 273.9 bits (699), Expect = 1.0e-69
Identity = 138/184 (75.00%), Postives = 150/184 (81.52%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD S ++MNR  PP P+PHR+VDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN LRLW
Sbjct: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60

Query: 61  RRRSA--GAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQL 120
           R RSA     A  V   NRT SSDVA AVKY K+EHCKEV +ECPVCLSAF DGEE+RQL
Sbjct: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120

Query: 121 TACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPDA 180
            ACKHSFHV CIDMWL SHPNCPVCRA++ VAVKRPI++ AP      R+DDFQQGLPDA
Sbjct: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180

[Close]

BLAST of Moc05g32170 vs. NCBI nr
Match: XP_008442561.1 (PREDICTED: RING-H2 finger protein ATL33 [Cucumis melo] >KAA0044082.1 RING-H2 finger protein ATL33 [Cucumis melo var. makuwa] >TYK25055.1 RING-H2 finger protein ATL33 [Cucumis melo var. makuwa])

HSP 1 Score: 272.7 bits (696), Expect = 2.2e-69
Identity = 135/184 (73.37%), Postives = 150/184 (81.52%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD++ + MNR  PP+P+PHR+VDFSPIEFILGLVAVITIPAL YSF FAIKCPPN LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 61  RRRSAGAGAGAV--AAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQL 120
           R+RS    A  V    GNRT SSDVA AVKY K+EHCKEVG+ECPVCLSAF DGEE+RQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 121 TACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPDA 180
           T CKHSFHV CIDMWL SHPNCPVCRA++ V VKRPI++ AP    A R+DDF QGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

[Close]

BLAST of Moc05g32170 vs. ExPASy Swiss-Prot
Match: O80927 (RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 6.0e-33
Identity = 75/202 (37.13%), Postives = 111/202 (54.95%), Query Frame = 0


Query: 3   SSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWRRR 62
           +ST ++  P PPFP+P RS+D +P++ I  ++A + +PALVY+ FF   C  +    RR 
Sbjct: 38  NSTFIIIGPPPPFPAPPRSIDLTPLKLIFVVIAFVAVPALVYALFFNGPCSSS----RRN 97

Query: 63  SAGAGAGAVAAGNRTFSSDVALAV----------KYKKEEHCKEVGSECPVCLSAFGDGE 122
           S+ +   + +      + D               K+ K+ H KE+G+EC VCL  F D +
Sbjct: 98  SSSSRTSSSSDDTPHATVDTPPITETTVTSESGGKFHKDTHSKEIGNECSVCLMVFTDSD 157

Query: 123 EVRQLTACKHSFHVACIDMWLCSHPNCPVCRASIAVA---------------VKRPISHR 180
           E+RQL+ CKH+FHV CI+ WL  HPNCP+CR  ++V                V R   +R
Sbjct: 158 ELRQLSECKHAFHVLCIETWLKDHPNCPICRTDVSVKQQTEAPNVPVNVNGNVNRSGGNR 217

[Close]

BLAST of Moc05g32170 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 8.2e-14
Identity = 47/123 (38.21%), Postives = 55/123 (44.72%), Query Frame = 0


Query: 60  RRRSAGAGAGAVAAG---------NRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGD 119
           RRRS   G G    G           T  S    A   K+EE   + G EC VCL+   D
Sbjct: 84  RRRSGSGGDGGTGGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEEDDDGVECAVCLAELED 143

Query: 120 GEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQ 174
           GEE R L  C H FH  C+DMWL SH  CP+CR ++ V    P     PVP      +  
Sbjct: 144 GEEARFLPRCGHGFHAECVDMWLGSHSTCPLCRLTVVV----PPPPLPPVPPEPPASYTV 202

[Close]

BLAST of Moc05g32170 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 9.0e-13
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 87  KYKKEEHCKEV-GSECPVCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRAS 146
           K KK ++  ++ G++C +CL  F + E +R L  C H+FHV CID WL SH NCP+CRA 
Sbjct: 140 KLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAK 199

Query: 147 IAVAVKRPISHRAPV 161
           I V   +   H   V
Sbjct: 200 IIVPTTQQPEHHVVV 214

[Close]

BLAST of Moc05g32170 vs. ExPASy Swiss-Prot
Match: Q67YI6 (RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 9.0e-13
Identity = 34/67 (50.75%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 100 ECPVCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRASI--AVAVKRPISHR 159
           +C VCL  F +G+ VR L  C H+FH+ CID WL SHPNCP+CR +I  +  V  P+S  
Sbjct: 155 DCAVCLLEFEEGDYVRTLPLCFHAFHLECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPF 214

Query: 160 APVPAPR 165
            P+ APR
Sbjct: 215 VPLMAPR 221

[Close]

BLAST of Moc05g32170 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 62/197 (31.47%), Postives = 88/197 (44.67%), Query Frame = 0


Query: 13  PPFPSPHRSVDFSPI---------EFILGLVAVITIPA----LVYSFFFAIKCPPN---L 72
           PP P P  +   SPI          F +  +AVI I A    LV  + F IKC  N   +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 73  RLWRRRSAGAGAGAVA-----AGNRTFSSDVALAV---KYKK------EEHCKEVGSECP 132
            ++RRR   +    +        NR        A+   K+KK      EE   +   EC 
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 133 VCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRASIAV-------AVKRPIS 173
           VCL+ F + E++R +  C H FH+ CID+WL  + NCP+CR S++         +  P S
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 195

[Close]

BLAST of Moc05g32170 vs. ExPASy TrEMBL
Match: A0A6J1CV70 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111015083 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.3e-99
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWR 60
           MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWR
Sbjct: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWR 60

Query: 61  RRSAGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQLTAC 120
           RRSAGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQLTAC
Sbjct: 61  RRSAGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQLTAC 120

Query: 121 KHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQGLPDAGTLV 180
           KHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQGLPDAGTLV
Sbjct: 121 KHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAPVPAPRNDDFQQGLPDAGTLV 179

[Close]

BLAST of Moc05g32170 vs. ExPASy TrEMBL
Match: A0A0A0LA67 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.7e-70
Identity = 138/185 (74.59%), Postives = 153/185 (82.70%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD++ + MNR  PP+P+PHR+VDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 61  RRRS---AGAGAGAVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQ 120
           R+RS   +GAGA  V  GNRT SSDVA AVKY K+EHCKEVG+ECPVCLSAF DGEE+RQ
Sbjct: 61  RQRSTRLSGAGA-TVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQ 120

Query: 121 LTACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPD 180
           L  CKHSFHV CIDMWL SHPNCPVCRA++ V VKRPI + AP    A R+DDF QGLPD
Sbjct: 121 LAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPD 180

[Close]

BLAST of Moc05g32170 vs. ExPASy TrEMBL
Match: A0A5D3DN65 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001760 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.1e-69
Identity = 135/184 (73.37%), Postives = 150/184 (81.52%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD++ + MNR  PP+P+PHR+VDFSPIEFILGLVAVITIPAL YSF FAIKCPPN LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 61  RRRSAGAGAGAV--AAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQL 120
           R+RS    A  V    GNRT SSDVA AVKY K+EHCKEVG+ECPVCLSAF DGEE+RQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 121 TACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPDA 180
           T CKHSFHV CIDMWL SHPNCPVCRA++ V VKRPI++ AP    A R+DDF QGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

[Close]

BLAST of Moc05g32170 vs. ExPASy TrEMBL
Match: A0A1S3B6Q9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.1e-69
Identity = 135/184 (73.37%), Postives = 150/184 (81.52%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD++ + MNR  PP+P+PHR+VDFSPIEFILGLVAVITIPAL YSF FAIKCPPN LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 61  RRRSAGAGAGAV--AAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQL 120
           R+RS    A  V    GNRT SSDVA AVKY K+EHCKEVG+ECPVCLSAF DGEE+RQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 121 TACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPDA 180
           T CKHSFHV CIDMWL SHPNCPVCRA++ V VKRPI++ AP    A R+DDF QGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

[Close]

BLAST of Moc05g32170 vs. ExPASy TrEMBL
Match: A0A6J1F238 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.0e-67
Identity = 134/184 (72.83%), Postives = 148/184 (80.43%), Query Frame = 0


Query: 1   MDSSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPN-LRLW 60
           MD+S A+MNR TPP P+PHR+ DFSPIEFILGL+AVI IPALVYSFFFAIKCPP  LRLW
Sbjct: 4   MDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLW 63

Query: 61  RRRSAGAGAG--AVAAGNRTFSSDVALAVKYKKEEHCKEVGSECPVCLSAFGDGEEVRQL 120
           RRRSA        V AGNR+  SDVA+  KY K+E  KEVG+ECPVCLSAF DGEE+RQL
Sbjct: 64  RRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123

Query: 121 TACKHSFHVACIDMWLCSHPNCPVCRASIAVAVKRPISHRAP--VPAPRNDDFQQGLPDA 180
            ACKHSFHV CIDMW  SHPNCPVCRA IA+AVKRPI++RAP    A R+DDF QGLPDA
Sbjct: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183

[Close]

BLAST of Moc05g32170 vs. TAIR 10
Match: AT2G37580.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 4.3e-34
Identity = 75/202 (37.13%), Postives = 111/202 (54.95%), Query Frame = 0


Query: 3   SSTAVMNRPTPPFPSPHRSVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLRLWRRR 62
           +ST ++  P PPFP+P RS+D +P++ I  ++A + +PALVY+ FF   C  +    RR 
Sbjct: 38  NSTFIIIGPPPPFPAPPRSIDLTPLKLIFVVIAFVAVPALVYALFFNGPCSSS----RRN 97

Query: 63  SAGAGAGAVAAGNRTFSSDVALAV----------KYKKEEHCKEVGSECPVCLSAFGDGE 122
           S+ +   + +      + D               K+ K+ H KE+G+EC VCL  F D +
Sbjct: 98  SSSSRTSSSSDDTPHATVDTPPITETTVTSESGGKFHKDTHSKEIGNECSVCLMVFTDSD 157

Query: 123 EVRQLTACKHSFHVACIDMWLCSHPNCPVCRASIAVA---------------VKRPISHR 180
           E+RQL+ CKH+FHV CI+ WL  HPNCP+CR  ++V                V R   +R
Sbjct: 158 ELRQLSECKHAFHVLCIETWLKDHPNCPICRTDVSVKQQTEAPNVPVNVNGNVNRSGGNR 217

[Close]

BLAST of Moc05g32170 vs. TAIR 10
Match: AT3G18930.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.4e-14
Identity = 34/67 (50.75%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 100 ECPVCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRASI--AVAVKRPISHR 159
           +C VCL  F +G+ VR L  C H+FH+ CID WL SHPNCP+CR +I  +  V  P+S  
Sbjct: 155 DCAVCLLEFEEGDYVRTLPLCFHAFHLECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPF 214

Query: 160 APVPAPR 165
            P+ APR
Sbjct: 215 VPLMAPR 221

[Close]

BLAST of Moc05g32170 vs. TAIR 10
Match: AT3G18930.2 (RING/U-box superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.4e-14
Identity = 34/67 (50.75%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 100 ECPVCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRASI--AVAVKRPISHR 159
           +C VCL  F +G+ VR L  C H+FH+ CID WL SHPNCP+CR +I  +  V  P+S  
Sbjct: 155 DCAVCLLEFEEGDYVRTLPLCFHAFHLECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPF 214

Query: 160 APVPAPR 165
            P+ APR
Sbjct: 215 VPLMAPR 221

[Close]

BLAST of Moc05g32170 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.4e-14
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 87  KYKKEEHCKEV-GSECPVCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRAS 146
           K KK ++  ++ G++C +CL  F + E +R L  C H+FHV CID WL SH NCP+CRA 
Sbjct: 140 KLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAK 199

Query: 147 IAVAVKRPISHRAPV 161
           I V   +   H   V
Sbjct: 200 IIVPTTQQPEHHVVV 214

[Close]

BLAST of Moc05g32170 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 8.4e-14
Identity = 62/197 (31.47%), Postives = 88/197 (44.67%), Query Frame = 0


Query: 13  PPFPSPHRSVDFSPI---------EFILGLVAVITIPA----LVYSFFFAIKCPPN---L 72
           PP P P  +   SPI          F +  +AVI I A    LV  + F IKC  N   +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 73  RLWRRRSAGAGAGAVA-----AGNRTFSSDVALAV---KYKK------EEHCKEVGSECP 132
            ++RRR   +    +        NR        A+   K+KK      EE   +   EC 
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 133 VCLSAFGDGEEVRQLTACKHSFHVACIDMWLCSHPNCPVCRASIAV-------AVKRPIS 173
           VCL+ F + E++R +  C H FH+ CID+WL  + NCP+CR S++         +  P S
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 195

The following BLAST results are available for this feature:

BLAST of Moc05g32170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145690.1	2.7e-99	100.00	RING-H2 finger protein ATL33 [Momordica charantia]	[more]
KAE8651013.1	7.7e-70	74.59	hypothetical protein Csa_001104 [Cucumis sativus]	[more]
XP_004137943.1	7.7e-70	74.59	RING-H2 finger protein ATL33 [Cucumis sativus]	[more]
XP_038905078.1	1.0e-69	75.00	RING-H2 finger protein ATL33 [Benincasa hispida]	[more]
XP_008442561.1	2.2e-69	73.37	PREDICTED: RING-H2 finger protein ATL33 [Cucumis melo] >KAA0044082.1 RING-H2 fin...	[more]

BLAST of Moc05g32170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80927	6.0e-33	37.13	RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2	[more]
Q9LRB7	8.2e-14	38.21	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
P0C041	9.0e-13	44.00	Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P...	[more]
Q67YI6	9.0e-13	50.75	RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2	[more]
P93823	1.2e-12	31.47	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]

BLAST of Moc05g32170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CV70	1.3e-99	100.00	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101508...	[more]
A0A0A0LA67	3.7e-70	74.59	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE...	[more]
A0A5D3DN65	1.1e-69	73.37	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B6Q9	1.1e-69	73.37	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 ...	[more]
A0A6J1F238	3.0e-67	72.83	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690...	[more]

BLAST of Moc05g32170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37580.1	4.3e-34	37.13	RING/U-box superfamily protein	[more]
AT3G18930.1	6.4e-14	50.75	RING/U-box superfamily protein	[more]
AT3G18930.2	6.4e-14	50.75	RING/U-box superfamily protein	[more]
AT4G17905.1	6.4e-14	44.00	RING/U-box superfamily protein	[more]
AT1G04360.1	8.4e-14	31.47	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 101..142 e-value: 1.1E-5 score: 34.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 100..143 e-value: 1.6E-12 score: 47.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 101..143 score: 12.120453
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 73..148 e-value: 6.2E-19 score: 69.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 159..179
None	No IPR available	PANTHER	PTHR45676:SF88	RING-H2 FINGER PROTEIN ATL33	coord: 8..179
None	No IPR available	CDD	cd16461	RING-H2_EL5_like	coord: 100..143 e-value: 4.51993E-16 score: 66.5647
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 88..149
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 8..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g32170.1	Moc05g32170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane