Moc05g28850 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc05g28850 vs. NCBI nr
Match: XP_022151368.1 (strigolactone esterase D14-like [Momordica charantia])

HSP 1 Score: 577.0 bits (1486), Expect = 8.8e-161
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS
Sbjct: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL
Sbjct: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180
           FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS
Sbjct: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240
           EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM
Sbjct: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240

Query: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD 284
           GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD
Sbjct: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD 283

BLAST of Moc05g28850 vs. NCBI nr
Match: XP_038905280.1 (strigolactone esterase D14-like [Benincasa hispida])

HSP 1 Score: 462.2 bits (1188), Expect = 3.2e-126
Identity = 225/284 (79.23%), Postives = 255/284 (89.79%), Query Frame = 0

Query: 1   MHGGFFNCGE-AAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFD 60
           M+GG FNCG  +A  EAL VNVYGNGT T+VLAHGFG DQS W Y+IPYLACFFKVVVFD
Sbjct: 1   MYGG-FNCGAGSAFAEALKVNVYGNGTETLVLAHGFGSDQSAWHYMIPYLACFFKVVVFD 60

Query: 61  SIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQ 120
            +FSPNVK ELYDE+KYSSFDGYA+DLVCVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQ
Sbjct: 61  LVFSPNVKLELYDERKYSSFDGYAKDLVCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQ 120

Query: 121 LFTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAI 180
           LF HLILLNASPRY+N +GY+GGF+RPQ+D IFKQMD NFT WVK FAPFAV VNDSKA+
Sbjct: 121 LFHHLILLNASPRYLNAEGYFGGFKRPQVDEIFKQMDNNFTKWVKEFAPFAVRVNDSKAM 180

Query: 181 SEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSK 240
           +EFEHSLGRM PKIAL+VAK VFLSDLR+LLPKVRTP++IILT KDNIVPKSVAF+MKS 
Sbjct: 181 AEFEHSLGRMNPKIALNVAKMVFLSDLRKLLPKVRTPTSIILTEKDNIVPKSVAFFMKSN 240

Query: 241 MGGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD 284
           MGG++NVKIL SEGHFPQL AYPQ LKVLS+VL+LK+  + S+D
Sbjct: 241 MGGNSNVKILKSEGHFPQLTAYPQLLKVLSKVLKLKEYKNYSRD 283

BLAST of Moc05g28850 vs. NCBI nr
Match: KAG6596658.1 (Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028196.1 Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 456.1 bits (1172), Expect = 2.3e-124
Identity = 224/280 (80.00%), Postives = 247/280 (88.21%), Query Frame = 0

Query: 4   GFFNCG-----EAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVF 63
           G FNCG      AAI EAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVF
Sbjct: 3   GGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVF 62

Query: 64  DSIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRP 123
           D +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRP
Sbjct: 63  DLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRP 122

Query: 124 QLFTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKA 183
           QLF HLILLNASPRY+N +GY GGFERP +D IF QMD+NFTNWVKNFAPFAV++ND K 
Sbjct: 123 QLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKT 182

Query: 184 ISEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKS 243
           I+EFEHSLGRMKP+IALSVAK VFLSDLR+LLPKV+TP+TII TRKDNIVPK VA Y+KS
Sbjct: 183 IAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKS 242

Query: 244 KMGGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKN 279
           K+G HA VKILNS+GHFPQL AYPQ LKVL+QVL LK KN
Sbjct: 243 KVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKN 282

BLAST of Moc05g28850 vs. NCBI nr
Match: XP_022945470.1 (strigolactone esterase D14-like [Cucurbita moschata])

HSP 1 Score: 456.1 bits (1172), Expect = 2.3e-124
Identity = 223/279 (79.93%), Postives = 247/279 (88.53%), Query Frame = 0

Query: 4   GFFNCG----EAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFD 63
           G FNCG     AAI EAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVFD
Sbjct: 3   GGFNCGGSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFD 62

Query: 64  SIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQ 123
            +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRPQ
Sbjct: 63  LVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQ 122

Query: 124 LFTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAI 183
           LF HLILLNASPRY+N +GY GGFERP +D IF QMD+NFTNWVKNFAPFAV++ND K I
Sbjct: 123 LFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTI 182

Query: 184 SEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSK 243
           +E EHSLGRMKP+IALSVAK VFLSDLR+LLPKV+TP+TII TRKDNIVPK VAFY+KSK
Sbjct: 183 AELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYIKSK 242

Query: 244 MGGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKN 279
           +G HA VKILNS+GHFPQL AYPQ LKVL+QVL L+ KN
Sbjct: 243 VGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQHKN 281

BLAST of Moc05g28850 vs. NCBI nr
Match: XP_008443004.1 (PREDICTED: strigolactone esterase D14-like [Cucumis melo] >KAA0043762.1 strigolactone esterase D14-like [Cucumis melo var. makuwa] >TYK25371.1 strigolactone esterase D14-like [Cucumis melo var. makuwa])

HSP 1 Score: 436.8 bits (1122), Expect = 1.4e-118
Identity = 209/275 (76.00%), Postives = 244/275 (88.73%), Query Frame = 0

Query: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AI EAL VNVYGNGT T+VLAHGFGLDQ+VW Y+IPYLACFFKVVVFD 
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180
           F HLILLNASPRY+N +GY GGFE+PQ+D IFK+MD+NFT W K F PFAV VN+SKA++
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIALSVAK VFLSDL +LLPKV+T ++IILT KDNIVPKSVAF++KS +
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLK 276
           GG++NV IL S+GHFPQL AYPQ LKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

BLAST of Moc05g28850 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.1e-60
Identity = 114/260 (43.85%), Postives = 169/260 (65.00%), Query Frame = 0

Query: 13  IVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           I+EALNV V G G   + LAHGFG DQS W  ++PY    ++VV++D + + +V  + +D
Sbjct: 6   ILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVNPDYFD 65

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +Y++ D Y  DL+ +++ L ++   YVGHS+SAMIG +A+  RP+LF+ LIL+  SPR
Sbjct: 66  FNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPR 125

Query: 133 YINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAISEFEHSLGRMKPK 192
           ++ND+ Y+GGFE  +++ +F  M+ N+  WV  FAP AV  +   A+ EF  +L  M+P 
Sbjct: 126 FLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPD 185

Query: 193 IALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNSE 252
           I+L V++ VF SDLR +L  VR P+ +I T KD  VP SVA Y++S +GG   V+ L +E
Sbjct: 186 ISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTE 245

Query: 253 GHFPQLIAYPQFLKVLSQVL 273
           GH PQL A  Q  + L + L
Sbjct: 246 GHLPQLSAPAQLAQFLRRAL 265

BLAST of Moc05g28850 vs. ExPASy Swiss-Prot
Match: Q10J20 (Probable esterase D14L OS=Oryza sativa subsp. japonica OX=39947 GN=D14L PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.5e-59
Identity = 113/248 (45.56%), Postives = 166/248 (66.94%), Query Frame = 0

Query: 13  IVEALNVNVYGNG-TATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELY 72
           + EA N+ V G G    +VLAHGFG DQSVW++++P+L   ++VV+FD++ +     + +
Sbjct: 4   VEEAHNLRVVGEGKRGVIVLAHGFGTDQSVWKHLVPHLVADYRVVLFDTMGAGPTNPDYF 63

Query: 73  DEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASP 132
           D  +Y++ +GYA DL+ +L+ L V   IYVGHS+SA+IG +A+  RP LF+ L+LL+ASP
Sbjct: 64  DFSRYATLEGYALDLLAILQELRVASCIYVGHSVSAVIGAIASISRPDLFSKLVLLSASP 123

Query: 133 RYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVN-DSKAISEFEHSLGRMK 192
           RY+ND  YYGGFE+  LD +F+ M  N+  W   FAP  V  + +S A+ EF  +L  ++
Sbjct: 124 RYLNDVDYYGGFEQEDLDELFEAMGSNYKAWCSGFAPLCVGGDMESVAVQEFSRTLFNIR 183

Query: 193 PKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILN 252
           P IALSVA+ +F SD+R LLP V  P  I+ + KD  VP  V+ Y+   +GG + V+++ 
Sbjct: 184 PDIALSVAQTIFQSDVRSLLPLVTVPCHIVQSTKDLAVPVVVSEYLHKHLGGDSIVEVMP 243

Query: 253 SEGHFPQL 259
           SEGH PQL
Sbjct: 244 SEGHLPQL 251

BLAST of Moc05g28850 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.5e-59
Identity = 114/254 (44.88%), Postives = 169/254 (66.54%), Query Frame = 0

Query: 13  IVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           I++A NV V G+G   +V AHGFG DQS W+ V+PY    +KV+++D + + +V  + +D
Sbjct: 5   ILDAFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSVNPDHFD 64

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
            ++Y++ D Y  DL+ +L+ LHV R  YVGHS+SAM G +A+  RP+LF+ LIL+ ASPR
Sbjct: 65  FRRYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGASPR 124

Query: 133 YIND-KGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAISEFEHSLGRMKP 192
           ++ND + Y+GGFE+ +++ +F  M+ N+  WV  FAP AV  +   A+ EF  +L  M+P
Sbjct: 125 FLNDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFNMRP 184

Query: 193 KIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNS 252
            I+L V++ VF SDLR +L  V  P  I+ T +D  VP SVA YMK  +GG + V+ L++
Sbjct: 185 DISLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQWLDT 244

Query: 253 EGHFPQLIAYPQFL 266
           EGH P L A P +L
Sbjct: 245 EGHLPHLSA-PSYL 257

BLAST of Moc05g28850 vs. ExPASy Swiss-Prot
Match: Q10QA5 (Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.3e-58
Identity = 112/263 (42.59%), Postives = 166/263 (63.12%), Query Frame = 0

Query: 11  AAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHEL 70
           A +++ LNV V G+G   VVL+HGFG DQS W  V+PYL    +VV++D + + +V  + 
Sbjct: 54  AKLLQILNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTRDHRVVLYDLVCAGSVNPDH 113

Query: 71  YDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNAS 130
           +D ++Y + D Y  DL+ +L+ L + R  +VGHS+SAMIG +A+  RP LF  L+L+ AS
Sbjct: 114 FDFRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRRPDLFAKLVLIGAS 173

Query: 131 PRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAISEFEHSLGRMK 190
           PR++ND  Y+GGFE  ++  +F  M  N++ W   +AP AV  +   A+ EF  +L  M+
Sbjct: 174 PRFLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADVPAAVQEFSRTLFNMR 233

Query: 191 PKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILN 250
           P I+L V + VF +DLR +L  VR P  ++ T +D  VP SVA Y+K+ +GG   V+ L 
Sbjct: 234 PDISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVAAYLKAHLGGRTTVEFLQ 293

Query: 251 SEGHFPQLIAYPQFLKVLSQVLR 274
           +EGH P L A      +L+QVLR
Sbjct: 294 TEGHLPHLSA----PSLLAQVLR 312

BLAST of Moc05g28850 vs. ExPASy Swiss-Prot
Match: Q9SZU7 (Probable esterase KAI2 OS=Arabidopsis thaliana OX=3702 GN=KAI2 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.7e-58
Identity = 110/262 (41.98%), Postives = 176/262 (67.18%), Query Frame = 0

Query: 13  IVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           + EA NV V G+G AT+VL HGFG DQSVW++++P+L   ++VV++D++ +     + +D
Sbjct: 4   VEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFD 63

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +YS+ +GY+ DL+ +LE L +E  I+VGHS+SAMIG +A+  RP LF+ +++++ASPR
Sbjct: 64  FDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPR 123

Query: 133 YINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVN-DSKAISEFEHSLGRMKP 192
           Y+ND  Y GGFE+  L+ +F+ +  N+  W   FAP AV  + DS A+ EF  +L  M+P
Sbjct: 124 YVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRP 183

Query: 193 KIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNS 252
            IALSV + +F SD+R++LP V  P  I+ + KD  VP  V+ Y+ + +G  + V+++ S
Sbjct: 184 DIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPS 243

Query: 253 EGHFPQLIAYPQFLKVLSQVLR 274
           +GH PQL +    + V+ + +R
Sbjct: 244 DGHLPQLSSPDSVIPVILRHIR 265

BLAST of Moc05g28850 vs. ExPASy TrEMBL
Match: A0A6J1DC02 (strigolactone esterase D14-like OS=Momordica charantia OX=3673 GN=LOC111019326 PE=4 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.3e-161
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS
Sbjct: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL
Sbjct: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180
           FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS
Sbjct: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240
           EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM
Sbjct: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240

Query: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD 284
           GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD
Sbjct: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD 283

BLAST of Moc05g28850 vs. ExPASy TrEMBL
Match: A0A6J1G109 (strigolactone esterase D14-like OS=Cucurbita moschata OX=3662 GN=LOC111449693 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.1e-124
Identity = 223/279 (79.93%), Postives = 247/279 (88.53%), Query Frame = 0

Query: 4   GFFNCG----EAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFD 63
           G FNCG     AAI EAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVFD
Sbjct: 3   GGFNCGGSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFD 62

Query: 64  SIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQ 123
            +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRPQ
Sbjct: 63  LVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQ 122

Query: 124 LFTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAI 183
           LF HLILLNASPRY+N +GY GGFERP +D IF QMD+NFTNWVKNFAPFAV++ND K I
Sbjct: 123 LFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTI 182

Query: 184 SEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSK 243
           +E EHSLGRMKP+IALSVAK VFLSDLR+LLPKV+TP+TII TRKDNIVPK VAFY+KSK
Sbjct: 183 AELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYIKSK 242

Query: 244 MGGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKN 279
           +G HA VKILNS+GHFPQL AYPQ LKVL+QVL L+ KN
Sbjct: 243 VGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQHKN 281

BLAST of Moc05g28850 vs. ExPASy TrEMBL
Match: A0A5A7TKE6 (Strigolactone esterase D14-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005110 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 6.9e-119
Identity = 209/275 (76.00%), Postives = 244/275 (88.73%), Query Frame = 0

Query: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AI EAL VNVYGNGT T+VLAHGFGLDQ+VW Y+IPYLACFFKVVVFD 
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180
           F HLILLNASPRY+N +GY GGFE+PQ+D IFK+MD+NFT W K F PFAV VN+SKA++
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIALSVAK VFLSDL +LLPKV+T ++IILT KDNIVPKSVAF++KS +
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLK 276
           GG++NV IL S+GHFPQL AYPQ LKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

BLAST of Moc05g28850 vs. ExPASy TrEMBL
Match: A0A1S3B719 (strigolactone esterase D14-like OS=Cucumis melo OX=3656 GN=LOC103486734 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 6.9e-119
Identity = 209/275 (76.00%), Postives = 244/275 (88.73%), Query Frame = 0

Query: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AI EAL VNVYGNGT T+VLAHGFGLDQ+VW Y+IPYLACFFKVVVFD 
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180
           F HLILLNASPRY+N +GY GGFE+PQ+D IFK+MD+NFT W K F PFAV VN+SKA++
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIALSVAK VFLSDL +LLPKV+T ++IILT KDNIVPKSVAF++KS +
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLK 276
           GG++NV IL S+GHFPQL AYPQ LKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

BLAST of Moc05g28850 vs. ExPASy TrEMBL
Match: A0A0A0LB72 (Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_3G792050 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 7.6e-118
Identity = 204/275 (74.18%), Postives = 246/275 (89.45%), Query Frame = 0

Query: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AI EALNVNVYGNG+ T+VLAHGFGLDQ+VW Y++PYLACFFKVVVFD 
Sbjct: 1   MYNGFNCGGGSAIAEALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180
           F HL+LLNASPRY+N +GY GGFE+PQ+D + K+MD+NFT W K FAPFAV VN+SKA++
Sbjct: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIAL+VAK VFLSDL +LLPKV+T ++IILT+KDNIVPKSVAF++KS +
Sbjct: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLK 276
           GG++NV IL S+GHFPQL A+PQ LKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLK 275

BLAST of Moc05g28850 vs. TAIR 10
Match: AT3G03990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 7.6e-62
Identity = 114/260 (43.85%), Postives = 169/260 (65.00%), Query Frame = 0

Query: 13  IVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           I+EALNV V G G   + LAHGFG DQS W  ++PY    ++VV++D + + +V  + +D
Sbjct: 6   ILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVNPDYFD 65

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +Y++ D Y  DL+ +++ L ++   YVGHS+SAMIG +A+  RP+LF+ LIL+  SPR
Sbjct: 66  FNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPR 125

Query: 133 YINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAISEFEHSLGRMKPK 192
           ++ND+ Y+GGFE  +++ +F  M+ N+  WV  FAP AV  +   A+ EF  +L  M+P 
Sbjct: 126 FLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPD 185

Query: 193 IALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNSE 252
           I+L V++ VF SDLR +L  VR P+ +I T KD  VP SVA Y++S +GG   V+ L +E
Sbjct: 186 ISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTE 245

Query: 253 GHFPQLIAYPQFLKVLSQVL 273
           GH PQL A  Q  + L + L
Sbjct: 246 GHLPQLSAPAQLAQFLRRAL 265

BLAST of Moc05g28850 vs. TAIR 10
Match: AT3G24420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 7.2e-60
Identity = 116/264 (43.94%), Postives = 174/264 (65.91%), Query Frame = 0

Query: 11  AAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHE- 70
           + +  A+N  + G+G  ++VLAHGFG DQSVW  +IP L+  FKV+VFD +FS  +K + 
Sbjct: 8   SGLASAMNAKIIGSGERSMVLAHGFGGDQSVWDKIIPVLSQSFKVLVFDWLFSGAIKDQT 67

Query: 71  LYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNA 130
           LYD  KY+S D ++ DL+ ++E L     ++VGHSMS +IGC A+  RP LFT+L+L+ A
Sbjct: 68  LYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCAASIKRPDLFTNLLLIAA 127

Query: 131 SPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAISEFEHSLGRM 190
           SPRYIN + Y GGFE   +D I   +  N+  W  +F+ F V   DS ++  FE SL +M
Sbjct: 128 SPRYINSEDYKGGFESKDIDTIITSIGSNYEAWAVDFSSFVVDSRDSLSVQRFEKSLKKM 187

Query: 191 KPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKIL 250
           KP+ AL++AK VF SD R +L +V  P  +I    D +VP SVA++M+ K+ G + V+I+
Sbjct: 188 KPETALALAKIVFGSDEREILGQVSVPCHVIQPGNDVVVPVSVAYFMQEKIKGKSTVEII 247

Query: 251 -NSEGHFPQLIAYPQFLKVLSQVL 273
            ++ GHFPQ+ ++ + L V+ ++L
Sbjct: 248 EDAIGHFPQMTSHLELLGVMRRLL 271

BLAST of Moc05g28850 vs. TAIR 10
Match: AT4G37470.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-59
Identity = 110/262 (41.98%), Postives = 176/262 (67.18%), Query Frame = 0

Query: 13  IVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           + EA NV V G+G AT+VL HGFG DQSVW++++P+L   ++VV++D++ +     + +D
Sbjct: 4   VEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFD 63

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +YS+ +GY+ DL+ +LE L +E  I+VGHS+SAMIG +A+  RP LF+ +++++ASPR
Sbjct: 64  FDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPR 123

Query: 133 YINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVN-DSKAISEFEHSLGRMKP 192
           Y+ND  Y GGFE+  L+ +F+ +  N+  W   FAP AV  + DS A+ EF  +L  M+P
Sbjct: 124 YVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRP 183

Query: 193 KIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNS 252
            IALSV + +F SD+R++LP V  P  I+ + KD  VP  V+ Y+ + +G  + V+++ S
Sbjct: 184 DIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPS 243

Query: 253 EGHFPQLIAYPQFLKVLSQVLR 274
           +GH PQL +    + V+ + +R
Sbjct: 244 DGHLPQLSSPDSVIPVILRHIR 265

BLAST of Moc05g28850 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.0e-05
Identity = 67/259 (25.87%), Postives = 108/259 (41.70%), Query Frame = 0

Query: 29  VVLAHGFGLDQSVWRYVIPYLACF---FKVVVFDSIFSPNVKHELYDEKKYSSFDGYARD 88
           ++L HGFG + ++W+Y   +L  F   F V V D +F         +  +  SF   AR 
Sbjct: 63  LLLLHGFGAN-AMWQYG-EHLRAFTGRFNVYVPDLLFFG--LSSTSEPNRTESFQ--ARC 122

Query: 89  LVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPRYINDKGYYGGFER 148
           L+ ++E   V+R   VG S    +G   A   P+    L+L  A           G F+ 
Sbjct: 123 LMRLMEAHGVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKV 182

Query: 149 PQLD---GI--------FKQMDQ-NFTNWVKNFAPFAVAVNDSKAISEFEHSLGRMKPKI 208
           P L+   GI         K++ + +F   +K    F +        +EF       K  +
Sbjct: 183 PNLEEATGILIPQTPEKLKELIRFSFVKPIKGVPSFFLWDFIDVMCTEFVEE----KRDL 242

Query: 209 ALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNSEG 268
             S+ K   LSD    LP+++  S II   +D I P  + + +K  +G  A + ++   G
Sbjct: 243 IKSILKDRRLSD----LPRIKQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAG 302

Query: 269 HFPQLIAYPQFLKVLSQVL 273
           H   L    +F+K L   L
Sbjct: 303 HAVNLEKSKEFVKHLKSFL 307

BLAST of Moc05g28850 vs. TAIR 10
Match: AT5G53050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.5e-04
Identity = 26/68 (38.24%), Postives = 36/68 (52.94%), Query Frame = 0

Query: 83  ARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPRYINDKGYYGG 142
           A D + +L+HL  +++  +GHSM AMI C  A   P+    L LLN +          GG
Sbjct: 107 ANDSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTG---------GG 165

Query: 143 FE-RPQLD 150
           FE  P+LD
Sbjct: 167 FECFPKLD 165