Moc05g28840 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g28840
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionserine/arginine-rich SC35-like splicing factor SCL33
Locationchr5: 21285863 .. 21290161 (-)
RNA-Seq ExpressionMoc05g28840
SyntenyMoc05g28840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGAGCGTTCGAGGTACAGAAAGGCGAAAGCCGAAGGAAGATCGAAGAATTTTGCTGTTCCACAGCGAGTGAGATTGAACGAAAAAGCTCCGTTGGCTTTCCGAATCCTCTGATTCTCAGGTTTTCGCCATCTCTTCTCTTTGTTTTCGCTTTGTTTCTCTTATCTTTCAATGTTTTCGCTAACGGAGATTCGATTTGATCGAGTTTTGATTTGACAAATAATGATACTATAATTTACTTATGCGTGAGATTATTTTCCCCCATATTTTATGCTGAATTATGCTTGCTATGAATCTGATCACATCTGAAGATCGGTAGATGTGAAAAAGGCTGCAACTATGCACGGATTTGTCTATTTGTCGTTGTTTTACTATAAGAATTGATCTTCCTTTGAGGTTCTGTTTGATTTGAGAAGAATTTTTGTGAGAATCTTTTGATTGCGTGGAAATATTTTGATCTCTCCGAAATCAATCTCCTTTGTTCTGCAGAGATATTGGAGCAGTTGATCGAAGCTTATGAGTAATGTGACTAGTTTGTCGCTCGATGAATCTTTTACATTTTTGTAATTTAGTACCGTTTTAAAATTTCTTAGCCTGTTAATGGTCTATCTTCTCGAAAATTCTTTTTCTATAATTGTTCTCGTTCCATTAAGAGAAAAAAATATCTCGCTGAATACTTCTTATGAGTGATGGCATAAATCTGTGGTAGCATGCTGATTGATGTGTTAGGAAATTTGGTCTATCTGCATCTTGTTGTGCTATTTGTTTTTAGGCACCATGATTTGTTTTAATTTGCCTGCCGTTGGTATAGGGAATTGAAAATGAGAGGTAGAAGTTACAGCTATAGTCCCTCCCCACCAAGGAGTTATGGTAGGAGACATCGTAGCCCTAGTCCACGGGATCGAGGAGGCCGTGGAAGGGATCTACCGACAAGCCTTTTGGTTCGGAACCTACGACATGACTGCAGGTTTGAAACTGCATTTTTCATTTTCTTTTCACTTAAGGGTTCAGATGCTAGGATTCTCTTTCTTTATATATGTATGTATGTATGGGTCTTTTAATCTTTCTAATGCAAGAGTTTCTTCCATTCACGATCTATTTTCCAGTTTTTTTCTTCCTTTGGTTTCAATTTCTTTATGATCCAATAAATCTCATTATGTAGAATTATGTGATGGTTTATGGATGTTTATCTTTTCATATTTGTTGATAAGAAGCTATTGTATCAAAGAAAGTTCAAACGCAAAAGGAGGAAGATAAGAAATCTACTATGCAGCCAAAAAGCTTACTAAAATGCACCTCAATTGGATAGAATCAAGAAATAACTACTATTATCACTTTTTTTTAATATCTGTGAGTGTCCGCATCTCAACTAATCTCACGGGACAACTGCTTAACCCTACTACATTAGGATGCCAAGGAAACTTTTAAAGCTATTATCACAAAAATTATTTGGATGATAAATACGAACTAGAAGTTAGAAATCTAATGAGATGCCAATGATAAGTATATATTTAAATTTTAAATTTGATTTCTCATTGAATATTTCAATTAAAACTGCAACGTACATCCAAATCTTGGCACTTATTTTTTTTTAATATTCCAGGGGTCTTTCACGTGTCAAGGTCTTTTATCCATACTATTATGTGTTGGTTGCTGCTACATTTCTCATCACATTTTTATTTTTACGTGCATGCTAAGGAGAGCACTAGGCCATGCTGTATGATGTACTTCAATATATGTATTTGTTGAAGTAAGAATAAAGGATTAAAGGGTTCTTTTACAAATATTTGTTCTCAGGTTGATACTTTTCTCAGCTTGATGTCTAGTTTTTTTTCCTATTAGAAGCAATAGAGATTTAAATTGGATTTTCCCACCCTTTCTTTGGAAATTTGAAGATCCTCGTTTGTTATTTTCTTTGAAATGTATTTTATATGATGTCTCATTGCTTGGTTGAGAATGTATTTTCTTTCTCAAAATGCGAAAACTTGTTAAGAAAAAGGACAAAAACTAAGTCATACAAGAACAGCCAAAAAAGACTCCAAGTAACTACGCTAGGCTGTAGGGAGCTTCAATTGTTGAGAACAAAAAGGACAAACGAAAAATGTGTTTGCTTTTGTTCTGTATTGAGTCGATTAACTTCTTCATACTTGCGTGTTTTGCAGGCCAGAGGACCTACGTGGGTTATTTGGGCGGTTTGGTCCTCTTAAGGACATTTACTTGCCACGAGATTATTATTCTGGGTAGGCATGGTGCATGGCTTTACGAGATTGTTCTTTACTTTGTATGCATCTGATGTGTGCGCGTCATGATATCTTTTCTGGGTAGTTATGTTTAACACAATTTAGTCCTGTTTTGTACGCATCTGGTCTTTATGCTTATGAATATACTTTCTGAATTTTTGTTTTTAATTTCCCATTTTCAAAGGAAACAATTTTATTGTTTAATATAAGCCCTATCCAATGAAGTTACAAAAAGTTTCTCCAATTGGATGAAGAGTGTACCAATAGAGTGTTCAAGCTATAATGGCTGAAAGGAGCCACATTTTACACCAAGTAGTAGCCAAAAGATAAAATATTGAACCAATAAAGAGTTGTTATGGGGAGTGTTCTTTTTCCTCAAAAAGCCTTGAATTTCACTCTCCAGGTGGCCCTTATGGAAGCTCTCAAGAAGTGTTGTGGGGAGTGTTCTTGTTCCTTAATATATTGTCTGGATAGTTACTCCTCTGGATTGCTTAATGGTTTGGAATTGGTCCTCTGTTTTCAATTATTTGGTTTTCCAATAACATACAATCTGTTTATGGAGTTTGGTTGTTTTATTGAAAGTGTTGAAGACAACCAATGAAGTAATAAGAGTATCTTGTGGAAGACATTCAAGTAAGTTCTGATGACTTAATGTGTACATGAAGATTCAGTGGAAGTTGGGTTTGAATTCATTGAGAAGAAGTTCAAGTCTTGAAGAATTTCATCTCACTTTTTTCTTTGCCACTCAGAAGGTTAGCATGGTGATGTTCAGATTCTCTTGTTTGAGTTCTCGGTAGACGTTTCTATCATTTCCAATTAATCTCTGGATTGATTGTTGATTGCAATGACGCCACTGGTCAAATTGACATTTATTGAAATTTTCCAAGTGGGTTAGTTAACATCCATTGTCAAAATTTGTATTTATGAATGTTATGATTGTGGTGCAGAGAGCCACGTGGCTTTGGATTTGTTCAGTTTGTTGATCCTGCTGATGCCACTGATGCCAAGTATGAACTGGATGGCCAAGTTCTCCTGGGTCGTGAATTAACTGTTGTATTTGCTGAGGAGAATAGGAAGAGACCTGAAGAGATGAGAGCAAGAGACTCATCCAGGTTGTCATCTCTTTCTTTAAACTGATTTCTGGTTGCCTATTTCTATTGCTTTGCTAAGCTAGGATGCGTGATGAAAAATTGATCTCTCAACTTCTATAAATTCCCTAGAAGCATCCTCAAAAATTTCATATGCCTCTTAAGTTTCTTTTTAGGCTGAGGCACAGCCAGCATTGGTCCTGAGATCTTTCTTCTCCTTGGATTTTCTTGAGTTTCCAATTTTGTAGAAAAAATGAGATCGAGGGTTGATCCCTTTTGTGAGATATGAATTCAAGGACTCTGAGTAAATTGACTTCACCATTCGATAACCACCATCTTTTGTCTTGTTAAGGAAAAGGAAAGTCTGTATGTTTGTTTTCAAAGACTACTGATCACCGTTGCAGTCTGTGTTCTTTTTTTATCAATCTATTGTCCATTTTTAAAATATCAATACTCATGTTTGTATTATTTGTTTGTGAAATTGTTACCAGGGGTCGGTCCTACAACTACAGGCGCTCTCCACTGCGATATTCTCGATCACCACACTATGATCGAAAATATTCACGTAGCCCAGAGTACTATTCCCCTGCTCAGTCTGGCCGATATTCACGGTGCGCAGTTATATGTATTCTTTTTCCATTATTAGATTTTATCCAAACAATTTTCGTAGCATATGATTTTCATCAAATGAATTAAGAAAAGAGAGTGCTAGTTAAGGCCTGTATCTCGGCCAGAAGGTCTGCAGTTTGAATCCCTACCCCACATTTTGGACTAAATAATACGTTGAACTAAATAATAATAGAGCGCATGGTGGTTTATTCTAACCTCATTTGTGTTATGTTAGGTCAATCTCACCAAGAGACCGGAGGTACAGGGAGCGGTCCTACTTGAGGTCACCTTATGATTCAAGGGGCCGCAGCTATAGCCGAAGCAGGAGCCGGAGCTATAGCCGAAGTTATTGTAGGTGA

mRNA sequence

ATGAGAGAGCGTTCGAGGTACAGAAAGGCGAAAGCCGAAGGAAGATCGAAGAATTTTGCTGTTCCACAGCGAAATTTTTGTGAGAATCTTTTGATTGCGTGGAAATATTTTGATCTCTCCGAAATCAATCTCCTTTGTTCTGCAGAGATATTGGAGCAGTTGATCGAAGCTTATGAGGAATTGAAAATGAGAGGTAGAAGTTACAGCTATAGTCCCTCCCCACCAAGGAGTTATGGTAGGAGACATCGTAGCCCTAGTCCACGGGATCGAGGAGGCCGTGGAAGGGATCTACCGACAAGCCTTTTGGTTCGGAACCTACGACATGACTGCAGGCCAGAGGACCTACGTGGGTTATTTGGGCGGTTTGGTCCTCTTAAGGACATTTACTTGCCACGAGATTATTATTCTGGAGAGCCACGTGGCTTTGGATTTGTTCAGTTTGTTGATCCTGCTGATGCCACTGATGCCAAGTATGAACTGGATGGCCAAGTTCTCCTGGGTCGTGAATTAACTGTTGTATTTGCTGAGGAGAATAGGAAGAGACCTGAAGAGATGAGAGCAAGAGACTCATCCAGGGGTCGGTCCTACAACTACAGGCGCTCTCCACTGCGATATTCTCGATCACCACACTATGATCGAAAATATTCACGTAGCCCAGAGTACTATTCCCCTGCTCAGTCTGGCCGATATTCACGGTCAATCTCACCAAGAGACCGGAGGTACAGGGAGCGGTCCTACTTGAGGTCACCTTATGATTCAAGGGGCCGCAGCTATAGCCGAAGCAGGAGCCGGAGCTATAGCCGAAGTTATTGTAGGTGA

Coding sequence (CDS)

ATGAGAGAGCGTTCGAGGTACAGAAAGGCGAAAGCCGAAGGAAGATCGAAGAATTTTGCTGTTCCACAGCGAAATTTTTGTGAGAATCTTTTGATTGCGTGGAAATATTTTGATCTCTCCGAAATCAATCTCCTTTGTTCTGCAGAGATATTGGAGCAGTTGATCGAAGCTTATGAGGAATTGAAAATGAGAGGTAGAAGTTACAGCTATAGTCCCTCCCCACCAAGGAGTTATGGTAGGAGACATCGTAGCCCTAGTCCACGGGATCGAGGAGGCCGTGGAAGGGATCTACCGACAAGCCTTTTGGTTCGGAACCTACGACATGACTGCAGGCCAGAGGACCTACGTGGGTTATTTGGGCGGTTTGGTCCTCTTAAGGACATTTACTTGCCACGAGATTATTATTCTGGAGAGCCACGTGGCTTTGGATTTGTTCAGTTTGTTGATCCTGCTGATGCCACTGATGCCAAGTATGAACTGGATGGCCAAGTTCTCCTGGGTCGTGAATTAACTGTTGTATTTGCTGAGGAGAATAGGAAGAGACCTGAAGAGATGAGAGCAAGAGACTCATCCAGGGGTCGGTCCTACAACTACAGGCGCTCTCCACTGCGATATTCTCGATCACCACACTATGATCGAAAATATTCACGTAGCCCAGAGTACTATTCCCCTGCTCAGTCTGGCCGATATTCACGGTCAATCTCACCAAGAGACCGGAGGTACAGGGAGCGGTCCTACTTGAGGTCACCTTATGATTCAAGGGGCCGCAGCTATAGCCGAAGCAGGAGCCGGAGCTATAGCCGAAGTTATTGTAGGTGA

Protein sequence

MRERSRYRKAKAEGRSKNFAVPQRNFCENLLIAWKYFDLSEINLLCSAEILEQLIEAYEELKMRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRRYRERSYLRSPYDSRGRSYSRSRSRSYSRSYCR
Homology
BLAST of Moc05g28840 vs. NCBI nr
Match: XP_022151394.1 (serine/arginine-rich SC35-like splicing factor SCL33 [Momordica charantia])

HSP 1 Score: 416.8 bits (1070), Expect = 1.5e-112
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF 122
           MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF
Sbjct: 1   MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF 60

Query: 123 GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP 182
           GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP
Sbjct: 61  GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP 120

Query: 183 EEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRRYR 242
           EEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRRYR
Sbjct: 121 EEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRRYR 180

Query: 243 ERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 273
           ERSYLRSPYDSRGRSYSRSRSRSYSRSYCR
Sbjct: 181 ERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 210

BLAST of Moc05g28840 vs. NCBI nr
Match: KAG6596659.1 (Serine/arginine-rich SC35-like splicing factor SCL30A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 370.2 bits (949), Expect = 1.6e-98
Identity = 189/213 (88.73%), Postives = 201/213 (94.37%), Query Frame = 0

Query: 60  ELKMRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 119
           +LKMRGRSYSYSPSPPRSYGRR+RS SPR  GGRGRDLPTSLLVRNLRHDCRPEDLRGLF
Sbjct: 45  KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 104

Query: 120 GRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENR 179
           GRFGPLKDIYLPRDYYSG+PRGFGFVQ++DP+DA DAKYELDGQVLLGRELTVVFAEENR
Sbjct: 105 GRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPSDAADAKYELDGQVLLGRELTVVFAEENR 164

Query: 180 KRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDR 239
           KRPE+MRARDSSRGRSY+YRRSPL+YS+SP + RKYSRSPEYYSPA+S RYSRSISPRDR
Sbjct: 165 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDR 224

Query: 240 RYRERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 273
           RYRERSY RSPYDSR R YSRSRSRS SRSY R
Sbjct: 225 RYRERSYSRSPYDSRDRGYSRSRSRSRSRSYSR 257

BLAST of Moc05g28840 vs. NCBI nr
Match: XP_022948454.1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 [Cucurbita moschata])

HSP 1 Score: 369.4 bits (947), Expect = 2.7e-98
Identity = 190/217 (87.56%), Postives = 203/217 (93.55%), Query Frame = 0

Query: 58  YEELKMRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRG 117
           Y++LKMRGRSYSYSPSPPRSYGRR+RS SPR  GGRGRDLPTSLLVRNLRHDCRPEDLRG
Sbjct: 6   YKKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRG 65

Query: 118 LFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEE 177
           LFGRFGPLKDIYLPRDYYSG+PRGFGFVQ++DP+DA DAKYELDGQVLLGRELTVVFAEE
Sbjct: 66  LFGRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPSDAADAKYELDGQVLLGRELTVVFAEE 125

Query: 178 NRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPR 237
           NRKRPE+MRARDSS GRSY+YRRSPL+YS+SP + RKYS SPEYYSPA+SGRYSRSISPR
Sbjct: 126 NRKRPEQMRARDSSSGRSYSYRRSPLQYSQSPPHGRKYSPSPEYYSPARSGRYSRSISPR 185

Query: 238 DRRYRERSYLRSPYDSRGRSY--SRSRSRSYSRSYCR 273
           DRRYRERSY RSPYDSR R Y  SRSRSRSYSRSY R
Sbjct: 186 DRRYRERSYSRSPYDSRDRGYSRSRSRSRSYSRSYSR 222

BLAST of Moc05g28840 vs. NCBI nr
Match: XP_008443002.1 (PREDICTED: serine/arginine-rich SC35-like splicing factor SCL33 [Cucumis melo])

HSP 1 Score: 367.9 bits (943), Expect = 7.8e-98
Identity = 193/212 (91.04%), Postives = 197/212 (92.92%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF 122
           MRGRSYSYSPSPPRSYGRR+RSPSPR RGGR RDLPTSLLVRNLRHDCRPEDLRGLFGRF
Sbjct: 1   MRGRSYSYSPSPPRSYGRRYRSPSPRSRGGRRRDLPTSLLVRNLRHDCRPEDLRGLFGRF 60

Query: 123 GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP 182
           GPLKDIYLPRDYYSGEPRGFGFVQFVD ADA DAKYELDGQVLLG ELTVVFAEENRKRP
Sbjct: 61  GPLKDIYLPRDYYSGEPRGFGFVQFVDAADAADAKYELDGQVLLGHELTVVFAEENRKRP 120

Query: 183 EEMRARDSSRGRSYNY--RRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRR 242
           EEMRARDSSRGRSY+Y  R SPLRYSRSPHYDRKYSRSPEYYSPA+S RYSR  SPR RR
Sbjct: 121 EEMRARDSSRGRSYSYSRRHSPLRYSRSPHYDRKYSRSPEYYSPARSRRYSR--SPRGRR 180

Query: 243 YRERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 273
           YRE+SY RSPYD RGRSYSRSRSRSYSRSY R
Sbjct: 181 YREQSYSRSPYDPRGRSYSRSRSRSYSRSYSR 210

BLAST of Moc05g28840 vs. NCBI nr
Match: XP_023539101.1 (serine/arginine-rich SC35-like splicing factor SCL33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 367.1 bits (941), Expect = 1.3e-97
Identity = 190/219 (86.76%), Postives = 203/219 (92.69%), Query Frame = 0

Query: 56  EAYEELKMRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDL 115
           + Y++LKMRGRSYSYSPSPPRSYGRR+RS SPR  GGRGRDLPTSLLVRNLRHDCRPEDL
Sbjct: 4   KVYKKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDL 63

Query: 116 RGLFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFA 175
           RGLFGRFGPLKDIYLPRDYYSG+PRGFGFVQ++DPADA DAKYELDGQVLLGRELTVVFA
Sbjct: 64  RGLFGRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFA 123

Query: 176 EENRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSIS 235
           EENRKRPE+MRARDSSRGRSY+YRRSPL+YS+SP + RKYSRSPEYYSPA+S RYSRSIS
Sbjct: 124 EENRKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSIS 183

Query: 236 PRDRRYRERSYLRSPYDSRGRSY--SRSRSRSYSRSYCR 273
           PRDRRYRE SY RSPYDSR R Y  SRSRSRSYS SY R
Sbjct: 184 PRDRRYREISYSRSPYDSRDRGYSRSRSRSRSYSGSYSR 222

BLAST of Moc05g28840 vs. ExPASy Swiss-Prot
Match: Q9SEU4 (Serine/arginine-rich SC35-like splicing factor SCL33 OS=Arabidopsis thaliana OX=3702 GN=SCL33 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 8.5e-63
Identity = 146/218 (66.97%), Postives = 164/218 (75.23%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDR-GGRGRDLPTSLLVRNLRHDCRPEDLRGLFGR 122
           MRGR  SY+PSPPR YGRR RSPSPR R GGR RDLPTSLLVRNLRHDCR EDLR  F +
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 123 FGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKR 182
           FGP+KDIYLPRDYY+G+PRGFGFVQF+DPADA DAK+ +DG +LLGRELTVVFAEENRK+
Sbjct: 61  FGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKK 120

Query: 183 PEEMRARDSSRGRSYNYRRSPLRY---SRS--PHYDRKYSRSPEYYSPAQSGRYSRSISP 242
           P EMRAR+   GR  + RR+P RY   SRS  P   R  SRS +YYSP     + RSISP
Sbjct: 121 PTEMRARERGGGRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYSPPPRRHHPRSISP 180

Query: 243 RDRRY-RERSYLRSPYD--SRGRSYS--RSRSRSYSRS 270
           R+ RY   RSY RSP    SRGRS +  R +SRS S S
Sbjct: 181 REERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPS 216

BLAST of Moc05g28840 vs. ExPASy Swiss-Prot
Match: Q9LHP2 (Serine/arginine-rich SC35-like splicing factor SCL30A OS=Arabidopsis thaliana OX=3702 GN=SCL30A PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.7e-61
Identity = 144/218 (66.06%), Postives = 163/218 (74.77%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDR--GGRGRDLPTSLLVRNLRHDCRPEDLRGLFG 122
           MRGR  SY+PSPPR YGRR RSPSPR R  G R  DLPTSLLVRNLRHDCR EDLR  F 
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRFGGSRDSDLPTSLLVRNLRHDCRQEDLRRPFE 60

Query: 123 RFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRK 182
           +FGP+KDIYLPRDYY+G+PRGFGF+QF+DPADA +AK+++DG +LLGRELTVVFAEENRK
Sbjct: 61  QFGPVKDIYLPRDYYTGDPRGFGFIQFMDPADAAEAKHQMDGYLLLGRELTVVFAEENRK 120

Query: 183 RPEEMRARDSSRGRSYNY---RRSPLRYSRSP---HYDRKYSRSPEYYSPAQSGRYSRSI 242
           +P EMR RD   GRS  +   RRSP RYSRSP      R  SRS  Y SP      SRS+
Sbjct: 121 KPTEMRTRDRG-GRSNRFQDRRRSPPRYSRSPPPRRGRRSRSRSRGYNSPPAKRHQSRSV 180

Query: 243 SPRDRRY-RERSYLRSP--YDSRGRSYSRSRSRSYSRS 270
           SP+DRRY +ERSY RSP    SR RS S  R +S+SRS
Sbjct: 181 SPQDRRYEKERSYSRSPPHNGSRVRSGSPGRVKSHSRS 215

BLAST of Moc05g28840 vs. ExPASy Swiss-Prot
Match: Q1PDV2 (Serine/arginine-rich SC35-like splicing factor SCL28 OS=Arabidopsis thaliana OX=3702 GN=SCL28 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.8e-34
Identity = 106/237 (44.73%), Postives = 132/237 (55.70%), Query Frame = 0

Query: 62  KMRGRSYSYSP-----SPPRSY-----GRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCR 121
           + R RS SYSP     SPPR        R    PS RD    G   P+ LL+RNL  D R
Sbjct: 3   RARSRSRSYSPRPRDRSPPRERKGYDDNRLRERPSSRDHESSG---PSGLLIRNLPLDAR 62

Query: 122 PEDLRGLFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELT 181
           P DLR  F RFGPLKDIYLPR+YY+GEPRGFGFV++    DA +A   ++ +V+ GRE+ 
Sbjct: 63  PNDLRDSFERFGPLKDIYLPRNYYTGEPRGFGFVKYRYAEDAAEAMKRMNHKVIGGREIA 122

Query: 182 VVFAEENRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSP------------ 241
           +VFAEENRK P+EMR  + + GR  +Y+R+  R  R  +     SRSP            
Sbjct: 123 IVFAEENRKTPQEMRTTNGTSGRHGDYKRTSHRSPRRRYRSHSRSRSPPRRESRHSKVRE 182

Query: 242 -EYYSPAQSGR-YSRSISPRDRRYRERSYLRSPYDS------RGRSYSRSRSRSYSR 269
            + YSP +  R  SRS  PR+ R  +    RSP +       R R  SRSRSRS SR
Sbjct: 183 DDLYSPRRRSRSISRSPLPRNEREYKSRNCRSPREERVLTPIRSRCLSRSRSRSLSR 236

BLAST of Moc05g28840 vs. ExPASy Swiss-Prot
Match: Q8L3X8 (Serine/arginine-rich SC35-like splicing factor SCL30 OS=Arabidopsis thaliana OX=3702 GN=SCL30 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 98/218 (44.95%), Postives = 120/218 (55.05%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSP----------RDRGGRGRDLPTSLLVRNLRHDCRP 122
           MR  S  Y   P R YG R RSP P             GGR      SLLVRN+  DCRP
Sbjct: 1   MRRYSPPYYSPPRRGYGGRGRSPPPPPPRRGYGGGGGGGGRRGSSHGSLLVRNIPLDCRP 60

Query: 123 EDLRGLFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTV 182
           E+LR  F RFGP++D+Y+PRDYYSG+PRGF FV+FVD  DA +A+  ++ +   GRE+TV
Sbjct: 61  EELREPFERFGPVRDVYIPRDYYSGQPRGFAFVEFVDAYDAGEAQRSMNRRSFAGREITV 120

Query: 183 VFAEENRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSR 242
           V A E+RKRPEEMR +  +R R  +  R    + RS       SRSP   S ++S   SR
Sbjct: 121 VVASESRKRPEEMRVKTRTRSREPSGSRD-RSHGRSRSRSISRSRSPRRPSDSRSRYRSR 180

Query: 243 SISPRDRRYRERSYLRSPYDSRGRSYSRSRSRSYSRSY 271
           S SP  RR         P      +YSR   RSYS  Y
Sbjct: 181 SYSPAPRRRG-----GPPRGEEDENYSR---RSYSPGY 209

BLAST of Moc05g28840 vs. ExPASy Swiss-Prot
Match: O75494 (Serine/arginine-rich splicing factor 10 OS=Homo sapiens OX=9606 GN=SRSF10 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.1e-25
Identity = 85/200 (42.50%), Postives = 113/200 (56.50%), Query Frame = 0

Query: 99  TSLLVRNLRHDCRPEDLRGLFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKY 158
           TSL VRN+  D R EDLR  FGR+GP+ D+Y+P D+Y+  PRGF +VQF D  DA DA +
Sbjct: 10  TSLFVRNVADDTRSEDLRREFGRYGPIVDVYVPLDFYTRRPRGFAYVQFEDVRDAEDALH 69

Query: 159 ELDGQVLLGRELTVVFAEENRKRPEEMRARDS-----------------SRGRSYNYRRS 218
            LD + + GR++ + FA+ +RK P +M+A++                  SR RSY  RRS
Sbjct: 70  NLDRKWICGRQIEIQFAQGDRKTPNQMKAKEGRNVYSSSRYDDYDRYRRSRSRSYERRRS 129

Query: 219 PLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDR-RYRERSYLRSPYDSRGRSYS- 273
               SRS  Y+ + S SP    P    R SRS S  DR ++R RS+ RS  +SR RS S 
Sbjct: 130 ---RSRSFDYNYRRSYSPRNSRPTGRPRRSRSHSDNDRFKHRNRSFSRSKSNSRSRSKSQ 189

BLAST of Moc05g28840 vs. ExPASy TrEMBL
Match: A0A6J1DCY5 (serine/arginine-rich SC35-like splicing factor SCL33 OS=Momordica charantia OX=3673 GN=LOC111019338 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 7.1e-113
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF 122
           MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF
Sbjct: 1   MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF 60

Query: 123 GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP 182
           GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP
Sbjct: 61  GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP 120

Query: 183 EEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRRYR 242
           EEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRRYR
Sbjct: 121 EEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRRYR 180

Query: 243 ERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 273
           ERSYLRSPYDSRGRSYSRSRSRSYSRSYCR
Sbjct: 181 ERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 210

BLAST of Moc05g28840 vs. ExPASy TrEMBL
Match: A0A6J1G9Y3 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452132 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.3e-98
Identity = 190/217 (87.56%), Postives = 203/217 (93.55%), Query Frame = 0

Query: 58  YEELKMRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRG 117
           Y++LKMRGRSYSYSPSPPRSYGRR+RS SPR  GGRGRDLPTSLLVRNLRHDCRPEDLRG
Sbjct: 6   YKKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRG 65

Query: 118 LFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEE 177
           LFGRFGPLKDIYLPRDYYSG+PRGFGFVQ++DP+DA DAKYELDGQVLLGRELTVVFAEE
Sbjct: 66  LFGRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPSDAADAKYELDGQVLLGRELTVVFAEE 125

Query: 178 NRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPR 237
           NRKRPE+MRARDSS GRSY+YRRSPL+YS+SP + RKYS SPEYYSPA+SGRYSRSISPR
Sbjct: 126 NRKRPEQMRARDSSSGRSYSYRRSPLQYSQSPPHGRKYSPSPEYYSPARSGRYSRSISPR 185

Query: 238 DRRYRERSYLRSPYDSRGRSY--SRSRSRSYSRSYCR 273
           DRRYRERSY RSPYDSR R Y  SRSRSRSYSRSY R
Sbjct: 186 DRRYRERSYSRSPYDSRDRGYSRSRSRSRSYSRSYSR 222

BLAST of Moc05g28840 vs. ExPASy TrEMBL
Match: A0A1S3B7U0 (serine/arginine-rich SC35-like splicing factor SCL33 OS=Cucumis melo OX=3656 GN=LOC103486733 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.8e-98
Identity = 193/212 (91.04%), Postives = 197/212 (92.92%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGRF 122
           MRGRSYSYSPSPPRSYGRR+RSPSPR RGGR RDLPTSLLVRNLRHDCRPEDLRGLFGRF
Sbjct: 1   MRGRSYSYSPSPPRSYGRRYRSPSPRSRGGRRRDLPTSLLVRNLRHDCRPEDLRGLFGRF 60

Query: 123 GPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKRP 182
           GPLKDIYLPRDYYSGEPRGFGFVQFVD ADA DAKYELDGQVLLG ELTVVFAEENRKRP
Sbjct: 61  GPLKDIYLPRDYYSGEPRGFGFVQFVDAADAADAKYELDGQVLLGHELTVVFAEENRKRP 120

Query: 183 EEMRARDSSRGRSYNY--RRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDRR 242
           EEMRARDSSRGRSY+Y  R SPLRYSRSPHYDRKYSRSPEYYSPA+S RYSR  SPR RR
Sbjct: 121 EEMRARDSSRGRSYSYSRRHSPLRYSRSPHYDRKYSRSPEYYSPARSRRYSR--SPRGRR 180

Query: 243 YRERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 273
           YRE+SY RSPYD RGRSYSRSRSRSYSRSY R
Sbjct: 181 YREQSYSRSPYDPRGRSYSRSRSRSYSRSYSR 210

BLAST of Moc05g28840 vs. ExPASy TrEMBL
Match: A0A6J1G9D2 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452132 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 8.4e-98
Identity = 189/215 (87.91%), Postives = 201/215 (93.49%), Query Frame = 0

Query: 60  ELKMRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 119
           +LKMRGRSYSYSPSPPRSYGRR+RS SPR  GGRGRDLPTSLLVRNLRHDCRPEDLRGLF
Sbjct: 2   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 61

Query: 120 GRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENR 179
           GRFGPLKDIYLPRDYYSG+PRGFGFVQ++DP+DA DAKYELDGQVLLGRELTVVFAEENR
Sbjct: 62  GRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPSDAADAKYELDGQVLLGRELTVVFAEENR 121

Query: 180 KRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSISPRDR 239
           KRPE+MRARDSS GRSY+YRRSPL+YS+SP + RKYS SPEYYSPA+SGRYSRSISPRDR
Sbjct: 122 KRPEQMRARDSSSGRSYSYRRSPLQYSQSPPHGRKYSPSPEYYSPARSGRYSRSISPRDR 181

Query: 240 RYRERSYLRSPYDSRGRSY--SRSRSRSYSRSYCR 273
           RYRERSY RSPYDSR R Y  SRSRSRSYSRSY R
Sbjct: 182 RYRERSYSRSPYDSRDRGYSRSRSRSRSYSRSYSR 216

BLAST of Moc05g28840 vs. ExPASy TrEMBL
Match: A0A6J1L3A1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498719 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 2.4e-97
Identity = 186/217 (85.71%), Postives = 203/217 (93.55%), Query Frame = 0

Query: 56  EAYEELKMRGRSYSYSPSPPRSYGRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCRPEDL 115
           + Y++LKMRGRSYSYSPSPPRSYGRR+RS SPR  GGRGRDLPTSLLVRNLRHDCRPEDL
Sbjct: 4   KVYKKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDL 63

Query: 116 RGLFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFA 175
           RGLFG+FGPLKDIYLPRDYYSG+PRGFGFVQ++DPADA DAKYELDGQVLLGRELTVVFA
Sbjct: 64  RGLFGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFA 123

Query: 176 EENRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSPEYYSPAQSGRYSRSIS 235
           EENRKRPE+MRARDSSRGRSY+YRRSPL+YS+SP + RKYSRSPEYYSPA+S RYSRSIS
Sbjct: 124 EENRKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSIS 183

Query: 236 PRDRRYRERSYLRSPYDSRGRSYSRSRSRSYSRSYCR 273
           PRD+ +RERSY RSPY+SR R YSRSRSRS SRSY R
Sbjct: 184 PRDQMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSR 220

BLAST of Moc05g28840 vs. TAIR 10
Match: AT1G55310.1 (SC35-like splicing factor 33 )

HSP 1 Score: 241.9 bits (616), Expect = 6.0e-64
Identity = 146/218 (66.97%), Postives = 164/218 (75.23%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDR-GGRGRDLPTSLLVRNLRHDCRPEDLRGLFGR 122
           MRGR  SY+PSPPR YGRR RSPSPR R GGR RDLPTSLLVRNLRHDCR EDLR  F +
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 123 FGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKR 182
           FGP+KDIYLPRDYY+G+PRGFGFVQF+DPADA DAK+ +DG +LLGRELTVVFAEENRK+
Sbjct: 61  FGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKK 120

Query: 183 PEEMRARDSSRGRSYNYRRSPLRY---SRS--PHYDRKYSRSPEYYSPAQSGRYSRSISP 242
           P EMRAR+   GR  + RR+P RY   SRS  P   R  SRS +YYSP     + RSISP
Sbjct: 121 PTEMRARERGGGRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYSPPPRRHHPRSISP 180

Query: 243 RDRRY-RERSYLRSPYD--SRGRSYS--RSRSRSYSRS 270
           R+ RY   RSY RSP    SRGRS +  R +SRS S S
Sbjct: 181 REERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPS 216

BLAST of Moc05g28840 vs. TAIR 10
Match: AT1G55310.2 (SC35-like splicing factor 33 )

HSP 1 Score: 241.5 bits (615), Expect = 7.9e-64
Identity = 144/216 (66.67%), Postives = 162/216 (75.00%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDR-GGRGRDLPTSLLVRNLRHDCRPEDLRGLFGR 122
           MRGR  SY+PSPPR YGRR RSPSPR R GGR RDLPTSLLVRNLRHDCR EDLR  F +
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 123 FGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRKR 182
           FGP+KDIYLPRDYY+G+PRGFGFVQF+DPADA DAK+ +DG +LLGRELTVVFAEENRK+
Sbjct: 61  FGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKK 120

Query: 183 PEEMRARDSSRGRSYNYRRSPLRY---SRS--PHYDRKYSRSPEYYSPAQSGRYSRSISP 242
           P EMRAR+   GR  + RR+P RY   SRS  P   R  SRS +YYSP     + RSISP
Sbjct: 121 PTEMRARERGGGRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYSPPPRRHHPRSISP 180

Query: 243 RDRRY-RERSYLRSPYD--SRGRSYSRSRSRSYSRS 270
           R+ RY   RSY RSP    SRGRS +  R +S S S
Sbjct: 181 REERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLS 214

BLAST of Moc05g28840 vs. TAIR 10
Match: AT3G13570.1 (SC35-like splicing factor 30A )

HSP 1 Score: 236.9 bits (603), Expect = 1.9e-62
Identity = 144/218 (66.06%), Postives = 163/218 (74.77%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDR--GGRGRDLPTSLLVRNLRHDCRPEDLRGLFG 122
           MRGR  SY+PSPPR YGRR RSPSPR R  G R  DLPTSLLVRNLRHDCR EDLR  F 
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRFGGSRDSDLPTSLLVRNLRHDCRQEDLRRPFE 60

Query: 123 RFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELTVVFAEENRK 182
           +FGP+KDIYLPRDYY+G+PRGFGF+QF+DPADA +AK+++DG +LLGRELTVVFAEENRK
Sbjct: 61  QFGPVKDIYLPRDYYTGDPRGFGFIQFMDPADAAEAKHQMDGYLLLGRELTVVFAEENRK 120

Query: 183 RPEEMRARDSSRGRSYNY---RRSPLRYSRSP---HYDRKYSRSPEYYSPAQSGRYSRSI 242
           +P EMR RD   GRS  +   RRSP RYSRSP      R  SRS  Y SP      SRS+
Sbjct: 121 KPTEMRTRDRG-GRSNRFQDRRRSPPRYSRSPPPRRGRRSRSRSRGYNSPPAKRHQSRSV 180

Query: 243 SPRDRRY-RERSYLRSP--YDSRGRSYSRSRSRSYSRS 270
           SP+DRRY +ERSY RSP    SR RS S  R +S+SRS
Sbjct: 181 SPQDRRYEKERSYSRSPPHNGSRVRSGSPGRVKSHSRS 215

BLAST of Moc05g28840 vs. TAIR 10
Match: AT1G55310.3 (SC35-like splicing factor 33 )

HSP 1 Score: 228.8 bits (582), Expect = 5.3e-60
Identity = 144/231 (62.34%), Postives = 161/231 (69.70%), Query Frame = 0

Query: 63  MRGRSYSYSPSPPRSYGRRHRSPSPRDR-GGRGRDLPTSLLVRNLRHDCRPEDLRGLFGR 122
           MRGR  SY+PSPPR YGRR RSPSPR R GGR RDLPTSLLVRNLRHDCR EDLR  F +
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 123 FGPLKDIYLPRDYYSG------------EPRGFGFVQFVDPADATDAKYELDGQVLLGRE 182
           FGP+KDIYLPRDYY+G            +PRGFGFVQF+DPADA DAK+ +DG +LLGRE
Sbjct: 61  FGPVKDIYLPRDYYTGSHLCTDTCKASRDPRGFGFVQFMDPADAADAKHHMDGYLLLGRE 120

Query: 183 LTVVFAEENRKRPEEMRARDSSRGRSYNYRR--SPLRYSRS----PHYDRKYSRSPEYYS 242
           LTVVFAEENRK+P EMRAR+   G  +  RR   P  YSRS    P   R  SRS +YYS
Sbjct: 121 LTVVFAEENRKKPTEMRARERGGGSRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYS 180

Query: 243 PAQSGRYSRSISPRDRRY-RERSYLRSPYD--SRGRSYS--RSRSRSYSRS 270
           P     + RSISPR+ RY   RSY RSP    SRGRS +  R +SRS S S
Sbjct: 181 PPPRRHHPRSISPREERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPS 229

BLAST of Moc05g28840 vs. TAIR 10
Match: AT5G18810.1 (SC35-like splicing factor 28 )

HSP 1 Score: 146.4 bits (368), Expect = 3.4e-35
Identity = 106/237 (44.73%), Postives = 132/237 (55.70%), Query Frame = 0

Query: 62  KMRGRSYSYSP-----SPPRSY-----GRRHRSPSPRDRGGRGRDLPTSLLVRNLRHDCR 121
           + R RS SYSP     SPPR        R    PS RD    G   P+ LL+RNL  D R
Sbjct: 3   RARSRSRSYSPRPRDRSPPRERKGYDDNRLRERPSSRDHESSG---PSGLLIRNLPLDAR 62

Query: 122 PEDLRGLFGRFGPLKDIYLPRDYYSGEPRGFGFVQFVDPADATDAKYELDGQVLLGRELT 181
           P DLR  F RFGPLKDIYLPR+YY+GEPRGFGFV++    DA +A   ++ +V+ GRE+ 
Sbjct: 63  PNDLRDSFERFGPLKDIYLPRNYYTGEPRGFGFVKYRYAEDAAEAMKRMNHKVIGGREIA 122

Query: 182 VVFAEENRKRPEEMRARDSSRGRSYNYRRSPLRYSRSPHYDRKYSRSP------------ 241
           +VFAEENRK P+EMR  + + GR  +Y+R+  R  R  +     SRSP            
Sbjct: 123 IVFAEENRKTPQEMRTTNGTSGRHGDYKRTSHRSPRRRYRSHSRSRSPPRRESRHSKVRE 182

Query: 242 -EYYSPAQSGR-YSRSISPRDRRYRERSYLRSPYDS------RGRSYSRSRSRSYSR 269
            + YSP +  R  SRS  PR+ R  +    RSP +       R R  SRSRSRS SR
Sbjct: 183 DDLYSPRRRSRSISRSPLPRNEREYKSRNCRSPREERVLTPIRSRCLSRSRSRSLSR 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151394.11.5e-112100.00serine/arginine-rich SC35-like splicing factor SCL33 [Momordica charantia][more]
KAG6596659.11.6e-9888.73Serine/arginine-rich SC35-like splicing factor SCL30A, partial [Cucurbita argyro... [more]
XP_022948454.12.7e-9887.56serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 [Cucurbita mosch... [more]
XP_008443002.17.8e-9891.04PREDICTED: serine/arginine-rich SC35-like splicing factor SCL33 [Cucumis melo][more]
XP_023539101.11.3e-9786.76serine/arginine-rich SC35-like splicing factor SCL33 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
Q9SEU48.5e-6366.97Serine/arginine-rich SC35-like splicing factor SCL33 OS=Arabidopsis thaliana OX=... [more]
Q9LHP22.7e-6166.06Serine/arginine-rich SC35-like splicing factor SCL30A OS=Arabidopsis thaliana OX... [more]
Q1PDV24.8e-3444.73Serine/arginine-rich SC35-like splicing factor SCL28 OS=Arabidopsis thaliana OX=... [more]
Q8L3X81.1e-3044.95Serine/arginine-rich SC35-like splicing factor SCL30 OS=Arabidopsis thaliana OX=... [more]
O754941.1e-2542.50Serine/arginine-rich splicing factor 10 OS=Homo sapiens OX=9606 GN=SRSF10 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1DCY57.1e-113100.00serine/arginine-rich SC35-like splicing factor SCL33 OS=Momordica charantia OX=3... [more]
A0A6J1G9Y31.3e-9887.56serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita mos... [more]
A0A1S3B7U03.8e-9891.04serine/arginine-rich SC35-like splicing factor SCL33 OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1G9D28.4e-9887.91serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita mos... [more]
A0A6J1L3A12.4e-9785.71serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita max... [more]
Match NameE-valueIdentityDescription
AT1G55310.16.0e-6466.97SC35-like splicing factor 33 [more]
AT1G55310.27.9e-6466.67SC35-like splicing factor 33 [more]
AT3G13570.11.9e-6266.06SC35-like splicing factor 30A [more]
AT1G55310.35.3e-6062.34SC35-like splicing factor 33 [more]
AT5G18810.13.4e-3544.73SC35-like splicing factor 28 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 100..173
e-value: 2.0E-22
score: 90.5
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 101..170
e-value: 1.6E-19
score: 69.5
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 99..177
score: 18.001007
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 94..202
e-value: 4.1E-26
score: 93.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..195
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..259
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..272
NoneNo IPR availablePANTHERPTHR23147SERINE/ARGININE RICH SPLICING FACTORcoord: 63..265
NoneNo IPR availablePANTHERPTHR23147:SF158SERINE/ARGININE-RICH SC35-LIKE SPLICING FACTOR SCL33coord: 63..265
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 99..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g28840.1Moc05g28840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016607 nuclear speck
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding