Moc05g28580 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g28580
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	patatin-like protein 2
Location	chr5: 21074126 .. 21075604 (+)
RNA-Seq Expression	Moc05g28580
Synteny	Moc05g28580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAACGTAGCCTTCCTTCACTTCCAGTACTTCTCGATGGAAATCAACAACCAACCGGACGGCGCCGACCTCACTTCCGAGAACCTCATCACGGTTCTCAACATCGACGGCGGCGGGATCAGAGGCCTTATTCCAGCCATCATGCTCGCCTTCCTCGAGTCTGAGTTGCAGGTAGTCTCTCTACTCCTTTCTTTCTTTCTTTCTCTTCCCACTTAATCTACTGCTTTTGATTTTTGAATGAATGGATGAAAAAACTGGACGGAGATGAAGCCAGAATCGCAGACTATTTTGATGTTGTCGCTGGAAGTAATACCGGAGGTATATTGGCGGCCATGCTAGCGGCGCCCAACGAGAACAACTGCCCTTTGTTCTCTGCCGACCAGCTGAAAGGGTTCTTCCTCCAACACTTTCCTCTAATATTCTCTCAGAAAGGATAAATTTTCCTTTCACTTTTTCGTTGTCCGTTGGTCTGTTGATCGCTTTTTTTTTGGTTTAATATAACCTTCTTTTCTGGCATGGTAGATGGCCGTGGTTTAGGAATTTGGTGGGTCGAAAGTACGGTAACGATTATCTCCGATCTCTGCTTCAGAAAACTTTCGGAAACACCAAACTAAATCAGACCATCACTAACCTTGTAGTACCAACATACGACCTTGAGCGCGAGTGTGGCACTGTATTTTCCAGTTTTGAGGTTGGTTCTAGATGTGTTTGTTCGGTTCGGGTGTGATTGATCAATAATTTGAATTTAACAAGTAATTAATGTAGGGGGGTTTGTTTAATTTTGCAGAAAAATTCCCTACTTAATGCTTTTCTGTCTGACATCTGCATCTCGGCCTCCTCAATTACCTTCCCATCGCTTCCAGACTCACCATCAGGGGAAGACGGAAGAGTTCGATCTCGTTGATACTGGAACTTGTGCAAATAGTCTGGTAGGAAATTTATTTAGCTGTGTTTCTATGATGAATTAATGAATGAATGAATTACTTACAGGCCGTGCTGGGGACATTCGAGAGGCTCAAGGGTTTCCGATCAGTAGTGCATAGGAGAAAATTTCTGATGATATCTCTCGGAAGTGGATTCCCCAAACAGCAACACGGCCTGAGAATACCCTGCTCCTCCACTCAAGATATTGAAGATATGTTTTGTCATCCTTCACAAAATGGGGATGTAAATCTGGGCATCCTTTTTCGAGGTACTGAATCCGAAGCCTACTATCTTCGTATTCACGAAGAAGTGCCCAGTGAAATCCTAGCATCAGCATCGGTAGACATGGCCACTACCAAGAATCTTAAGCGACTCGTAAAATTCGGCGAACAGTTGTTGGACAAGCCTGTCTCCAAGGAGGGGATATTTTATATGAAGGATGCAGTGCACAAGGGTTACCCCTCTGAAACCAATCGCCAAGCTTTGGTCAGGTTCGCCAAATTGCTCTCACACAACAAAAGGTCTTCTTCTACCAACAACACCACAACTTGA

mRNA sequence

ATGGAGAACGTAGCCTTCCTTCACTTCCAGTACTTCTCGATGGAAATCAACAACCAACCGGACGGCGCCGACCTCACTTCCGAGAACCTCATCACGGTTCTCAACATCGACGGCGGCGGGATCAGAGGCCTTATTCCAGCCATCATGCTCGCCTTCCTCGAGTCTGAGTTGCAGGCCGTGCTGGGGACATTCGAGAGGCTCAAGGGTTTCCGATCAGTAGTGCATAGGAGAAAATTTCTGATGATATCTCTCGGAAGTGGATTCCCCAAACAGCAACACGGCCTGAGAATACCCTGCTCCTCCACTCAAGATATTGAAGATATGTTTTGTCATCCTTCACAAAATGGGGATGTAAATCTGGGCATCCTTTTTCGAGGTACTGAATCCGAAGCCTACTATCTTCGTATTCACGAAGAAGTGCCCAGTGAAATCCTAGCATCAGCATCGGTAGACATGGCCACTACCAAGAATCTTAAGCGACTCGTAAAATTCGGCGAACAGTTGTTGGACAAGCCTGTCTCCAAGGAGGGGATATTTTATATGAAGGATGCAGTGCACAAGGGTTACCCCTCTGAAACCAATCGCCAAGCTTTGGTCAGGTTCGCCAAATTGCTCTCACACAACAAAAGGTCTTCTTCTACCAACAACACCACAACTTGA

Coding sequence (CDS)

ATGGAGAACGTAGCCTTCCTTCACTTCCAGTACTTCTCGATGGAAATCAACAACCAACCGGACGGCGCCGACCTCACTTCCGAGAACCTCATCACGGTTCTCAACATCGACGGCGGCGGGATCAGAGGCCTTATTCCAGCCATCATGCTCGCCTTCCTCGAGTCTGAGTTGCAGGCCGTGCTGGGGACATTCGAGAGGCTCAAGGGTTTCCGATCAGTAGTGCATAGGAGAAAATTTCTGATGATATCTCTCGGAAGTGGATTCCCCAAACAGCAACACGGCCTGAGAATACCCTGCTCCTCCACTCAAGATATTGAAGATATGTTTTGTCATCCTTCACAAAATGGGGATGTAAATCTGGGCATCCTTTTTCGAGGTACTGAATCCGAAGCCTACTATCTTCGTATTCACGAAGAAGTGCCCAGTGAAATCCTAGCATCAGCATCGGTAGACATGGCCACTACCAAGAATCTTAAGCGACTCGTAAAATTCGGCGAACAGTTGTTGGACAAGCCTGTCTCCAAGGAGGGGATATTTTATATGAAGGATGCAGTGCACAAGGGTTACCCCTCTGAAACCAATCGCCAAGCTTTGGTCAGGTTCGCCAAATTGCTCTCACACAACAAAAGGTCTTCTTCTACCAACAACACCACAACTTGA

Protein sequence

MENVAFLHFQYFSMEINNQPDGADLTSENLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVVHRRKFLMISLGSGFPKQQHGLRIPCSSTQDIEDMFCHPSQNGDVNLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT

Homology

[Close]

BLAST of Moc05g28580 vs. NCBI nr
Match: XP_022159387.1 (patatin-like protein 2 [Momordica charantia])

HSP 1 Score: 323.2 bits (827), Expect = 1.8e-84
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 59  AVLGTFERLKGFRSVVHRRKFLMISLGSGFPKQQHGLRIPCSSTQDIEDMFCHPSQNGDV 118
           AVLGTFERLKGFRSVVHRRKFLMISLGSGFPKQQHGLRIPCSSTQDIEDMFCHPSQNGDV
Sbjct: 43  AVLGTFERLKGFRSVVHRRKFLMISLGSGFPKQQHGLRIPCSSTQDIEDMFCHPSQNGDV 102

Query: 119 NLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGI 178
           NLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGI
Sbjct: 103 NLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGI 162

Query: 179 FYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT 220
           FYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT
Sbjct: 163 FYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT 203

[Close]

BLAST of Moc05g28580 vs. NCBI nr
Match: PKI46096.1 (hypothetical protein CRG98_033491 [Punica granatum])

HSP 1 Score: 112.8 bits (281), Expect = 3.6e-21
Identity = 82/207 (39.61%), Postives = 107/207 (51.69%), Query Frame = 0


Query: 26  TSENLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFR---------SVVHR 85
           T  NLIT+L+IDGGGIRGLIP  +LAFLESELQ ++   E  K              +  
Sbjct: 13  TYGNLITILSIDGGGIRGLIPGTILAFLESELQTLVAMGEVTKEIMGGSSDFFAIKPMDY 72

Query: 86  RKFLMISLGSGFPKQQH-------------GLRIPCSSTQDIEDMFCHPSQNGDVNLGIL 145
           R+FL+ISLG+G PK Q              G       T  ++      S   D +L  +
Sbjct: 73  RRFLVISLGTGAPKYQEKYSADEAAKWGVLGWLAGGGGTPLVDVFMQSSSDMVDFHLSAV 132

Query: 146 FRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKD 205
           FR    E  YLRI ++  S  +  +SVD+AT KNL  LVK GE LL KPV    +   K 
Sbjct: 133 FRALHIEENYLRIQDDTLSGEV--SSVDVATRKNLDDLVKVGESLLQKPVCHVNLDTGK- 192

Query: 206 AVHKGYPSETNRQALVRFAKLLSHNKR 211
              +    ET+ +A+ RFAK+LSH +R
Sbjct: 193 --CEARGQETSAEAIRRFAKILSHERR 214

[Close]

BLAST of Moc05g28580 vs. NCBI nr
Match: CBI19551.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 103.6 bits (257), Expect = 2.2e-18
Identity = 75/197 (38.07%), Postives = 107/197 (54.31%), Query Frame = 0


Query: 26  TSENLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVVHRRKF-LMISL 85
           T  NLIT+L+IDGGGIRGLIP  +L FLESELQ + G   R+  +  V+       +++ 
Sbjct: 13  TYGNLITILSIDGGGIRGLIPGTVLGFLESELQKLDGEDARISDYFDVIAGTSTGGLVTA 72

Query: 86  GSGFPKQQHGLRIPCSSTQDIEDMFCHP-----SQNGDVNLGILFRGTESEAYYLRIHEE 145
               P +  G   P  S +DI+D +         Q+    +  LF+   SE  YLRI ++
Sbjct: 73  MLTTPNENTGR--PLFSAKDIKDFYLDHCPKIFPQHSHDPIPHLFQAFHSEKSYLRIQDD 132

Query: 146 VPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDAVHKGYPSETNRQALV 205
             S I  ++SVD+AT +NL  LVK GE+LL K VS+     +   + +    ETN +AL 
Sbjct: 133 TLSGI--TSSVDIATKENLDDLVKIGEELLKKRVSRVN---LDTGIFEPSNHETNEEALT 192

Query: 206 RFAKLLSHNKRSSSTNN 217
            FA+LLS  K+   T +
Sbjct: 193 SFARLLSQEKQRRDTRS 202

[Close]

BLAST of Moc05g28580 vs. NCBI nr
Match: XP_009627385.2 (patatin-like protein 2 [Nicotiana tomentosiformis])

HSP 1 Score: 100.5 bits (249), Expect = 1.9e-17
Identity = 71/208 (34.13%), Postives = 113/208 (54.33%), Query Frame = 0


Query: 30  LITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRS---------VVHRRKFL 89
           LIT+L+IDGGGIRG+IPA +L +LES+LQA++ T +      +          +   +FL
Sbjct: 268 LITILSIDGGGIRGIIPATILEYLESQLQALVATSQVTHQIMTGNPDFFPIKPIDYGRFL 327

Query: 90  MISLGSGFPK--QQHGLRIPC----------SSTQDIEDMFCHPSQN-GDVNLGILFRGT 149
           +IS+G+G  K  Q++  +I              +  I D+F   S +  D+++ ++F+  
Sbjct: 328 VISIGTGSAKVEQKYNAKIASKWGILGWLLNGGSTPIVDVFTQASGDMVDLHISVVFQSL 387

Query: 150 ESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDAVHK 209
            SE  YLRI ++  +     +SVD+AT +N+ +LV+ G+ LL KPVS+  +        +
Sbjct: 388 HSEENYLRIQDDTLTG--TDSSVDIATKENMDKLVEIGKTLLKKPVSRVNL--QTGLSEQ 447

Query: 210 GYPSETNRQALVRFAKLLSHNKRSSSTN 216
                TN + L RFAK+LS  KR    N
Sbjct: 448 CKNGGTNEETLKRFAKVLSEEKRLRERN 471

[Close]

BLAST of Moc05g28580 vs. NCBI nr
Match: PKU69488.1 (Patatin-2-Kuras 1 [Dendrobium catenatum])

HSP 1 Score: 100.1 bits (248), Expect = 2.4e-17
Identity = 74/214 (34.58%), Postives = 114/214 (53.27%), Query Frame = 0


Query: 29  NLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV-------------- 88
           +L+TVL+IDGGG+RG+IP I+LAFLES+LQ + G   RL  +  V+              
Sbjct: 15  SLVTVLSIDGGGVRGIIPGIILAFLESKLQELDGEEARLADYFDVIAGTSTGGLVTTMLT 74

Query: 89  -----HRRKFLMISLGSGFPKQQHGLRIPCSST------------QDIEDMFCHPSQN-G 148
                   KFL++S+G+G  K +       ++               I D++   S +  
Sbjct: 75  APNKMEYSKFLVLSVGTGSAKAEENYNAKDAAKWGVLRWLYNNGGTPIIDIYSQASADMV 134

Query: 149 DVNLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 208
           D+   ILF+   SE  YLRI ++  + + +++SVD++TT+NL+ LVK GE L++K VS+ 
Sbjct: 135 DIMTCILFKTLHSENNYLRIQDD--TLMGSTSSVDISTTENLEELVKIGENLIEKNVSRV 194

Query: 209 GIFYMKDAVHKGYPSETNRQALVRFAKLLSHNKR 211
            I        KG    TN +AL+ FA LLS  ++
Sbjct: 195 NIETGIYEPVKG--GGTNEEALIHFAHLLSKERK 224

[Close]

BLAST of Moc05g28580 vs. ExPASy Swiss-Prot
Match: O48723 (Patatin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PLP2 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.3e-09
Identity = 45/146 (30.82%), Postives = 74/146 (50.68%), Query Frame = 0


Query: 78  KFLMISLGSGFPKQQHGLR------------IPCSSTQDIEDMFCHPSQN-GDVNLGILF 137
           +FL++SLG+G  K +                +   ++  I D F   S +  D +L  +F
Sbjct: 252 RFLVLSLGTGNHKAEEKFNAKEVAGWGLLNWLTHDNSTPIIDAFSQASSDMVDFHLSAVF 311

Query: 138 RGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDA 197
           R   SEA Y+RI ++  +    +ASVD+AT +NL  L K G++LL KPV++     +   
Sbjct: 312 RALHSEANYIRIQDDTLTG--DAASVDIATVENLDILAKTGDELLKKPVARVN---LDSG 371

Query: 198 VHKGYPSETNRQALVRFAKLLSHNKR 211
            ++     TN  AL++ A +LS  K+
Sbjct: 372 CNENAYETTNEHALIKLAGILSKEKK 392

[Close]

BLAST of Moc05g28580 vs. ExPASy Swiss-Prot
Match: A2YW91 (Patatin-like protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=PLP2 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.7e-09
Identity = 51/148 (34.46%), Postives = 74/148 (50.00%), Query Frame = 0


Query: 78  KFLMISLGSGFPKQQHGLRIPCS-----------STQDIEDMFCHPSQN-GDVNLGILFR 137
           KF++IS+G G    Q       +           S+  I DMF   S +  D++LG+LF 
Sbjct: 257 KFMVISIGCGSNHDQKYKAKDAAKWGIFNWLIKGSSAPIIDMFTSASADMVDIHLGVLFS 316

Query: 138 GTESEAYYLRIHEEVPSEILASA-SVDMATTKNLKRLVKFGEQLLDKPVSKEGI--FYMK 197
             + E  YLRI  +   ++  SA S+D  + +N+  LVK GE LLDK VS+  +   +  
Sbjct: 317 ALQCEKNYLRIQYD---QLTGSAGSIDDCSKENMDNLVKIGEMLLDKNVSRVDLETGHYV 376

Query: 198 DAVHKGYPSETNRQALVRFAKLLSHNKR 211
           D   +G    TNR  L +FAK LS  +R
Sbjct: 377 DVAGEG----TNRDQLAKFAKQLSDERR 397

[Close]

BLAST of Moc05g28580 vs. ExPASy Swiss-Prot
Match: Q6ZJD3 (Patatin-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP2 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.7e-09
Identity = 51/148 (34.46%), Postives = 74/148 (50.00%), Query Frame = 0


Query: 78  KFLMISLGSGFPKQQHGLRIPCS-----------STQDIEDMFCHPSQN-GDVNLGILFR 137
           KF++IS+G G    Q       +           S+  I DMF   S +  D++LG+LF 
Sbjct: 257 KFMVISIGCGSNHDQKYKAKDAAKWGIFNWLIKGSSAPIIDMFTSASADMVDIHLGVLFS 316

Query: 138 GTESEAYYLRIHEEVPSEILASA-SVDMATTKNLKRLVKFGEQLLDKPVSKEGI--FYMK 197
             + E  YLRI  +   ++  SA S+D  + +N+  LVK GE LLDK VS+  +   +  
Sbjct: 317 ALQCEKNYLRIQYD---QLTGSAGSIDDCSKENMDNLVKIGEMLLDKNVSRVDLETGHYV 376

Query: 198 DAVHKGYPSETNRQALVRFAKLLSHNKR 211
           D   +G    TNR  L +FAK LS  +R
Sbjct: 377 DVAGEG----TNRDQLAKFAKQLSDERR 397

[Close]

BLAST of Moc05g28580 vs. ExPASy Swiss-Prot
Match: O23181 (Patatin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLP3 PE=2 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-07
Identity = 57/202 (28.22%), Postives = 92/202 (45.54%), Query Frame = 0


Query: 27  SENLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVVHRRKFLMISLGS 86
           SE +    N+  GGI    P +      +E+   +     + G  S +   +FL+IS+G+
Sbjct: 228 SEGIKHEFNLIDGGIAANNPTLCAI---AEVTKQIIKKNPVMGDISPLDFTRFLVISIGT 287

Query: 87  GFPKQQHGLRIPCSSTQDIEDMFCHPSQNG----------------DVNLGILFRGTESE 146
           G  + Q       +S      + C   ++G                D    ++F+   SE
Sbjct: 288 GSIRNQEKYNAKMASKW---GLMCWVFESGSTPILDCYSEAIHDMVDYQSSVVFQALRSE 347

Query: 147 AYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDAVHKGYP 206
             YLRI ++     L   SVD++T KN++ LV+ GE LL K VS+  +    ++ H    
Sbjct: 348 KNYLRIDDDSLKGDL--GSVDISTEKNMEGLVEVGEALLKKRVSRVNL----ESGHYQPI 407

Query: 207 SE--TNRQALVRFAKLLSHNKR 211
           SE  TN +AL RFAK+LS  ++
Sbjct: 408 SENVTNEEALKRFAKVLSEERK 417

HSP 2 Score: 50.8 bits (120), Expect = 2.2e-05
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 0


Query: 30 LITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV 75
          L+T+L+IDGGGIRG+IP  +LA+LES+LQ + G   RL  +  V+
Sbjct: 34 LVTILSIDGGGIRGIIPGTILAYLESQLQELDGEEARLVDYFDVI 78

[Close]

BLAST of Moc05g28580 vs. ExPASy Swiss-Prot
Match: Q3YJT0 (Patatin-2-Kuras 4 OS=Solanum tuberosum OX=4113 GN=pat2-k4 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 9.0e-07
Identity = 52/184 (28.26%), Postives = 83/184 (45.11%), Query Frame = 0


Query: 36  IDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVVHRRKFLMISLGSG-------- 95
           +DGG      PA++   + ++L  V   F  +K     +  ++ L++SLG+G        
Sbjct: 202 VDGGVATVGDPALLSLSVATKLAQVDPKFASIKS----LDYKQMLLLSLGTGTNSEFDKT 261

Query: 96  FPKQQHGLRIPCSSTQDIEDMF-CHPSQNGDVNLGILFRGTESEAYYLRIHEEVPSEILA 155
           +  Q+     P      I+ M     S   D  +  +F+   S+  YLR+ E   +    
Sbjct: 262 YTAQETAKWGPLRWMLAIQQMTNAASSYMTDYYISTVFQARHSQNNYLRVQENALTG--T 321

Query: 156 SASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDAVHKGYPSETNRQALVRFAKLLS 211
           +  +D A+  N++ LV+ GE LL KPVSK+               ET  +AL RFAKLLS
Sbjct: 322 TTEMDDASEANMELLVQVGETLLKKPVSKDS-------------PETYEEALKRFAKLLS 366

[Close]

BLAST of Moc05g28580 vs. ExPASy TrEMBL
Match: A0A6J1DZQ3 (patatin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025803 PE=4 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 8.6e-85
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 59  AVLGTFERLKGFRSVVHRRKFLMISLGSGFPKQQHGLRIPCSSTQDIEDMFCHPSQNGDV 118
           AVLGTFERLKGFRSVVHRRKFLMISLGSGFPKQQHGLRIPCSSTQDIEDMFCHPSQNGDV
Sbjct: 43  AVLGTFERLKGFRSVVHRRKFLMISLGSGFPKQQHGLRIPCSSTQDIEDMFCHPSQNGDV 102

Query: 119 NLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGI 178
           NLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGI
Sbjct: 103 NLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGI 162

Query: 179 FYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT 220
           FYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT
Sbjct: 163 FYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT 203

[Close]

BLAST of Moc05g28580 vs. ExPASy TrEMBL
Match: A0A2I0IRM5 (Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_033491 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.8e-21
Identity = 82/207 (39.61%), Postives = 107/207 (51.69%), Query Frame = 0


Query: 26  TSENLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFR---------SVVHR 85
           T  NLIT+L+IDGGGIRGLIP  +LAFLESELQ ++   E  K              +  
Sbjct: 13  TYGNLITILSIDGGGIRGLIPGTILAFLESELQTLVAMGEVTKEIMGGSSDFFAIKPMDY 72

Query: 86  RKFLMISLGSGFPKQQH-------------GLRIPCSSTQDIEDMFCHPSQNGDVNLGIL 145
           R+FL+ISLG+G PK Q              G       T  ++      S   D +L  +
Sbjct: 73  RRFLVISLGTGAPKYQEKYSADEAAKWGVLGWLAGGGGTPLVDVFMQSSSDMVDFHLSAV 132

Query: 146 FRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKD 205
           FR    E  YLRI ++  S  +  +SVD+AT KNL  LVK GE LL KPV    +   K 
Sbjct: 133 FRALHIEENYLRIQDDTLSGEV--SSVDVATRKNLDDLVKVGESLLQKPVCHVNLDTGK- 192

Query: 206 AVHKGYPSETNRQALVRFAKLLSHNKR 211
              +    ET+ +A+ RFAK+LSH +R
Sbjct: 193 --CEARGQETSAEAIRRFAKILSHERR 214

[Close]

BLAST of Moc05g28580 vs. ExPASy TrEMBL
Match: A0A2I0W1I3 (Patatin OS=Dendrobium catenatum OX=906689 GN=pat2-k1 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.2e-17
Identity = 74/214 (34.58%), Postives = 114/214 (53.27%), Query Frame = 0


Query: 29  NLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV-------------- 88
           +L+TVL+IDGGG+RG+IP I+LAFLES+LQ + G   RL  +  V+              
Sbjct: 15  SLVTVLSIDGGGVRGIIPGIILAFLESKLQELDGEEARLADYFDVIAGTSTGGLVTTMLT 74

Query: 89  -----HRRKFLMISLGSGFPKQQHGLRIPCSST------------QDIEDMFCHPSQN-G 148
                   KFL++S+G+G  K +       ++               I D++   S +  
Sbjct: 75  APNKMEYSKFLVLSVGTGSAKAEENYNAKDAAKWGVLRWLYNNGGTPIIDIYSQASADMV 134

Query: 149 DVNLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 208
           D+   ILF+   SE  YLRI ++  + + +++SVD++TT+NL+ LVK GE L++K VS+ 
Sbjct: 135 DIMTCILFKTLHSENNYLRIQDD--TLMGSTSSVDISTTENLEELVKIGENLIEKNVSRV 194

Query: 209 GIFYMKDAVHKGYPSETNRQALVRFAKLLSHNKR 211
            I        KG    TN +AL+ FA LLS  ++
Sbjct: 195 NIETGIYEPVKG--GGTNEEALIHFAHLLSKERK 224

[Close]

BLAST of Moc05g28580 vs. ExPASy TrEMBL
Match: A0A166AZS8 (Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_010916 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 7.2e-15
Identity = 75/220 (34.09%), Postives = 109/220 (49.55%), Query Frame = 0


Query: 18  NQPDGADLTSENLITVLNIDGGGIRGLIPAIMLAFLESELQ---AVLGTF-ERLKG-FRS 77
           N+P     T   ++TVL+IDGGGIRG+IP  +LAFLES+LQ   A+   F E LKG F  
Sbjct: 15  NKPTSHQTTKGKIVTVLSIDGGGIRGIIPGTILAFLESKLQTQVALTQIFNEILKGNFEF 74

Query: 78  V----VHRRKFLMISLGSGFPKQQHGLRIPC-------------SSTQDIEDMFCHPSQN 137
           +    +   K L++SLG+G  K +                     +T  I+      +  
Sbjct: 75  IDIKPMDTTKMLVLSLGTGAAKLEAKYNASMVAQWSPINWIFDKGATPLIDVYSASSADM 134

Query: 138 GDVNLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSK 197
            D+ +  LF+   +E  YLRI ++  + I  + S D+ATT N++ L   G +LL+K V++
Sbjct: 135 VDIQVSSLFQSLGAEKNYLRIQDD--NLIGNTTSADIATTTNMEALADIGNKLLEKSVAR 194

Query: 198 EGIFYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTN 216
             I     A        TN  AL RFAKLLS  ++    N
Sbjct: 195 VNI--DTGAFEPVVGEGTNSDALTRFAKLLSDERKIRIAN 230

[Close]

BLAST of Moc05g28580 vs. ExPASy TrEMBL
Match: A0A6P5U4T8 (Patatin OS=Prunus avium OX=42229 GN=LOC110773891 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 9.4e-15
Identity = 77/271 (28.41%), Postives = 116/271 (42.80%), Query Frame = 0


Query: 26  TSENLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV----------- 85
           T  N++TVL+IDGGGIRG+IP  +L FLESELQ + G   RL  +  V+           
Sbjct: 19  TYGNIVTVLSIDGGGIRGIIPGTILKFLESELQKLDGEEARLADYFDVIAGTSTGGLVTA 78

Query: 86  ------------------------------------------------------------ 145
                                                                       
Sbjct: 79  MLTTPDENDRPLFAAKDINNFYLEHCPKIFPQDSNYTALVAISEVTKEIHQGNSDFFAIR 138

Query: 146 --HRRKFLMISLGSGFPKQQH----------GLR--IPCSSTQDIEDMFCHPSQN-GDVN 205
                +FL+ISLG+G  K +           GL   +    +  + D+F   S +  D +
Sbjct: 139 STEYGRFLVISLGTGSAKSEEKYNADDAAKWGLLGWLTKGGSSPLTDVFTQASNDMVDFH 198

Query: 206 LGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIF 211
           L  +FR  + E  YLRI ++   + +  +SVD+AT +NL  LVK GE+LL KPVS+    
Sbjct: 199 LATVFRALDCEKNYLRIQDDTLEKTV--SSVDIATQENLNNLVKVGEELLKKPVSRVN-- 258

[Close]

BLAST of Moc05g28580 vs. TAIR 10
Match: AT2G26560.1 (phospholipase A 2A )

HSP 1 Score: 63.2 bits (152), Expect = 3.1e-10
Identity = 45/146 (30.82%), Postives = 74/146 (50.68%), Query Frame = 0


Query: 78  KFLMISLGSGFPKQQHGLR------------IPCSSTQDIEDMFCHPSQN-GDVNLGILF 137
           +FL++SLG+G  K +                +   ++  I D F   S +  D +L  +F
Sbjct: 252 RFLVLSLGTGNHKAEEKFNAKEVAGWGLLNWLTHDNSTPIIDAFSQASSDMVDFHLSAVF 311

Query: 138 RGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDA 197
           R   SEA Y+RI ++  +    +ASVD+AT +NL  L K G++LL KPV++     +   
Sbjct: 312 RALHSEANYIRIQDDTLTG--DAASVDIATVENLDILAKTGDELLKKPVARVN---LDSG 371

Query: 198 VHKGYPSETNRQALVRFAKLLSHNKR 211
            ++     TN  AL++ A +LS  K+
Sbjct: 372 CNENAYETTNEHALIKLAGILSKEKK 392

[Close]

BLAST of Moc05g28580 vs. TAIR 10
Match: AT4G37050.1 (PATATIN-like protein 4 )

HSP 1 Score: 57.8 bits (138), Expect = 1.3e-08
Identity = 57/202 (28.22%), Postives = 92/202 (45.54%), Query Frame = 0


Query: 27  SENLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVVHRRKFLMISLGS 86
           SE +    N+  GGI    P +      +E+   +     + G  S +   +FL+IS+G+
Sbjct: 228 SEGIKHEFNLIDGGIAANNPTLCAI---AEVTKQIIKKNPVMGDISPLDFTRFLVISIGT 287

Query: 87  GFPKQQHGLRIPCSSTQDIEDMFCHPSQNG----------------DVNLGILFRGTESE 146
           G  + Q       +S      + C   ++G                D    ++F+   SE
Sbjct: 288 GSIRNQEKYNAKMASKW---GLMCWVFESGSTPILDCYSEAIHDMVDYQSSVVFQALRSE 347

Query: 147 AYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGIFYMKDAVHKGYP 206
             YLRI ++     L   SVD++T KN++ LV+ GE LL K VS+  +    ++ H    
Sbjct: 348 KNYLRIDDDSLKGDL--GSVDISTEKNMEGLVEVGEALLKKRVSRVNL----ESGHYQPI 407

Query: 207 SE--TNRQALVRFAKLLSHNKR 211
           SE  TN +AL RFAK+LS  ++
Sbjct: 408 SENVTNEEALKRFAKVLSEERK 417

HSP 2 Score: 50.8 bits (120), Expect = 1.6e-06
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 0


Query: 30 LITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV 75
          L+T+L+IDGGGIRG+IP  +LA+LES+LQ + G   RL  +  V+
Sbjct: 34 LVTILSIDGGGIRGIIPGTILAYLESQLQELDGEEARLVDYFDVI 78

[Close]

BLAST of Moc05g28580 vs. TAIR 10
Match: AT4G37070.2 (Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.9e-06
Identity = 21/46 (45.65%), Postives = 34/46 (73.91%), Query Frame = 0


Query: 29 NLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV 75
          +L+T+L++DGGG+RG+I  ++LAFLE +LQ + G   RL  +  V+
Sbjct: 17 SLVTILSLDGGGVRGIIAGVILAFLEKQLQELDGEEARLADYFDVI 62

[Close]

BLAST of Moc05g28580 vs. TAIR 10
Match: AT4G37070.3 (Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.9e-06
Identity = 21/46 (45.65%), Postives = 34/46 (73.91%), Query Frame = 0


Query: 29 NLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV 75
          +L+T+L++DGGG+RG+I  ++LAFLE +LQ + G   RL  +  V+
Sbjct: 17 SLVTILSLDGGGVRGIIAGVILAFLEKQLQELDGEEARLADYFDVI 62

[Close]

BLAST of Moc05g28580 vs. TAIR 10
Match: AT4G37070.1 (Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.9e-06
Identity = 21/46 (45.65%), Postives = 34/46 (73.91%), Query Frame = 0


Query: 29 NLITVLNIDGGGIRGLIPAIMLAFLESELQAVLGTFERLKGFRSVV 75
          +L+T+L++DGGG+RG+I  ++LAFLE +LQ + G   RL  +  V+
Sbjct: 17 SLVTILSLDGGGVRGIIAGVILAFLEKQLQELDGEEARLADYFDVI 62

The following BLAST results are available for this feature:

BLAST of Moc05g28580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159387.1	1.8e-84	100.00	patatin-like protein 2 [Momordica charantia]	[more]
PKI46096.1	3.6e-21	39.61	hypothetical protein CRG98_033491 [Punica granatum]	[more]
CBI19551.3	2.2e-18	38.07	unnamed protein product, partial [Vitis vinifera]	[more]
XP_009627385.2	1.9e-17	34.13	patatin-like protein 2 [Nicotiana tomentosiformis]	[more]
PKU69488.1	2.4e-17	34.58	Patatin-2-Kuras 1 [Dendrobium catenatum]	[more]

BLAST of Moc05g28580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48723	4.3e-09	30.82	Patatin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PLP2 PE=1 SV=1	[more]
A2YW91	5.7e-09	34.46	Patatin-like protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=PLP2 PE=3 SV=1	[more]
Q6ZJD3	5.7e-09	34.46	Patatin-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP2 PE=3 SV=...	[more]
O23181	1.8e-07	28.22	Patatin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLP3 PE=2 SV=2	[more]
Q3YJT0	9.0e-07	28.26	Patatin-2-Kuras 4 OS=Solanum tuberosum OX=4113 GN=pat2-k4 PE=2 SV=1	[more]

BLAST of Moc05g28580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZQ3	8.6e-85	100.00	patatin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025803 PE=4 SV=1	[more]
A0A2I0IRM5	1.8e-21	39.61	Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_033491 PE=4 SV=1	[more]
A0A2I0W1I3	1.2e-17	34.58	Patatin OS=Dendrobium catenatum OX=906689 GN=pat2-k1 PE=3 SV=1	[more]
A0A166AZS8	7.2e-15	34.09	Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_010916 ...	[more]
A0A6P5U4T8	9.4e-15	28.41	Patatin OS=Prunus avium OX=42229 GN=LOC110773891 PE=3 SV=1	[more]

BLAST of Moc05g28580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26560.1	3.1e-10	30.82	phospholipase A 2A	[more]
AT4G37050.1	1.3e-08	28.22	PATATIN-like protein 4	[more]
AT4G37070.2	7.9e-06	45.65	Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein	[more]
AT4G37070.3	7.9e-06	45.65	Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein	[more]
AT4G37070.1	7.9e-06	45.65	Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.1090.10	Cytosolic phospholipase A2 catalytic domain	coord: 66..218 e-value: 3.7E-13 score: 51.4 coord: 23..65 e-value: 8.4E-7 score: 30.5
None	No IPR available	PANTHER	PTHR32176:SF68	PATATIN-LIKE PROTEIN 1	coord: 77..213
None	No IPR available	PANTHER	PTHR32176:SF68	PATATIN-LIKE PROTEIN 1	coord: 26..70
None	No IPR available	PANTHER	PTHR32176	XYLOSE ISOMERASE	coord: 77..213
None	No IPR available	PANTHER	PTHR32176	XYLOSE ISOMERASE	coord: 26..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g28580.1	Moc05g28580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds