Moc05g28200 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g28200
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr5: 20708370 .. 20708861 (-)
RNA-Seq ExpressionMoc05g28200
SyntenyMoc05g28200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTCGCAAGAATCGATATTGACAAGTTCGATGGTAAAGGTGATTTTGAGTTATGGAAGGTCAAAATTAAAGCACTTCTGGGACAACATAAAGCATCCAAGGCCATTCTAGATCAAGCAAAGTTATTGGAGACAATGACTGCATAAGAAAGGGAAACAATGGAAGAAATTGCATACGGGACAATCATCCTCAACCTCAGTGATAACGTTCTAAGGCAAGTAATGGAGGTTGAAACTGCATACAAGATATGGACGAAGTTGGAAGAAATTTTTCTCTCTAGAGAATTACCAAACAAGGTATACATCAAAGAGAAATATTTCACTTACAAGATGGGCAACTCAAAAACCCTAAGTGAGAATCTTGACGACTTCAAGAAACTATCATCAGAATTCAAAAGCTTAGGGGAAAAGATTGGTGATGACAACGAAGCTTTCATCCCTTTGAACTCACTTCCTGAGGCATATAAAGAAGTGAAACTCACTTCCTGA

mRNA sequence

ATGGCAGTCGCAAGAATCGATATTGACAAGTTCGATGGTAAAGGTGATTTTGAGTTATGGAAGGTCAAAATTAAAGCACTTCTGGGACAACATAAAGCATCCAAGGCCATTCTAGATCAAGCAAATGATAACGTTCTAAGGCAAGTAATGGAGGTTGAAACTGCATACAAGATATGGACGAAGTTGGAAGAAATTTTTCTCTCTAGAGAATTACCAAACAAGGTATACATCAAAGAGAAATATTTCACTTACAAGATGGGCAACTCAAAAACCCTAAGTGAGAATCTTGACGACTTCAAGAAACTATCATCAGAATTCAAAAGCTTAGGGGAAAAGATTGGTGATGACAACGAAGCTTTCATCCCTTTGAACTCACTTCCTGAGGCATATAAAGAAGTGAAACTCACTTCCTGA

Coding sequence (CDS)

ATGGCAGTCGCAAGAATCGATATTGACAAGTTCGATGGTAAAGGTGATTTTGAGTTATGGAAGGTCAAAATTAAAGCACTTCTGGGACAACATAAAGCATCCAAGGCCATTCTAGATCAAGCAAATGATAACGTTCTAAGGCAAGTAATGGAGGTTGAAACTGCATACAAGATATGGACGAAGTTGGAAGAAATTTTTCTCTCTAGAGAATTACCAAACAAGGTATACATCAAAGAGAAATATTTCACTTACAAGATGGGCAACTCAAAAACCCTAAGTGAGAATCTTGACGACTTCAAGAAACTATCATCAGAATTCAAAAGCTTAGGGGAAAAGATTGGTGATGACAACGAAGCTTTCATCCCTTTGAACTCACTTCCTGAGGCATATAAAGAAGTGAAACTCACTTCCTGA

Protein sequence

MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASKAILDQANDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSENLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVKLTS
Homology
BLAST of Moc05g28200 vs. NCBI nr
Match: XP_038896323.1 (uncharacterized protein LOC120084587 [Benincasa hispida])

HSP 1 Score: 161.8 bits (408), Expect = 4.3e-36
Identity = 75/144 (52.08%), Postives = 109/144 (75.69%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASKAILD----------QANDNVLRQVM 60
           M++AR +++KFDGKGDF LWK KIKA+L Q KA +A+L+          Q  ++      
Sbjct: 1   MSIARFEVEKFDGKGDFGLWKAKIKAILDQQKAHRALLNPSTLSATMTAQEKEDWELATY 60

Query: 61  EVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSENLDDFKKLSSEFKSLG 120
           + ETAY+IWTKLEE++ +++LP+K+Y+ EK+FT+KM +SKTL+ N D+FKK+ +EFK+LG
Sbjct: 61  DQETAYRIWTKLEELYATKDLPSKMYLSEKFFTFKMDSSKTLTNNFDEFKKIVAEFKTLG 120

Query: 121 EKIGDDNEAFIPLNSLPEAYKEVK 135
           EK+ D NEA++  NSLPE+YKE+K
Sbjct: 121 EKLSDKNEAYVLPNSLPESYKEIK 144

BLAST of Moc05g28200 vs. NCBI nr
Match: XP_038889933.1 (uncharacterized protein LOC120079695 [Benincasa hispida])

HSP 1 Score: 154.5 bits (389), Expect = 6.8e-34
Identity = 76/162 (46.91%), Postives = 109/162 (67.28%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASKAILD--------------------- 60
           M   R +I+KFDGK DFELWK KIKA+LGQ KA  AI D                     
Sbjct: 1   MTTTRFEIEKFDGKTDFELWKAKIKAVLGQQKALLAITDLTKYPKTFTDVEKEIIEVNAY 60

Query: 61  -----QANDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSE 120
                   DNVLRQ+++ +TA+ +W KL EI+L+++L NK + ++++FTYKM  +K+L+E
Sbjct: 61  GTIVLNVTDNVLRQIVDRQTAFDLWNKLNEIYLNKDLLNKAFSRKRFFTYKMDYAKSLTE 120

Query: 121 NLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVKLT 137
           NL++FK+LS EF+S+G+ IG++NE FI LNSLP+++K+VK T
Sbjct: 121 NLNEFKRLSLEFRSIGDNIGEENEVFILLNSLPDSFKDVKAT 162

BLAST of Moc05g28200 vs. NCBI nr
Match: XP_038890043.1 (uncharacterized protein LOC120079747 [Benincasa hispida])

HSP 1 Score: 154.1 bits (388), Expect = 8.9e-34
Identity = 79/160 (49.38%), Postives = 107/160 (66.88%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASKAILDQA------------------- 60
           MA  R +I+KFD K DFELWKVKIK +LGQ KA  AI D A                   
Sbjct: 1   MATTRYEIEKFDSKTDFELWKVKIKVVLGQQKALLAITDPAKYPETLTEAEKETIEVNAC 60

Query: 61  -------NDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSE 120
                   DNVLRQV+   TAY +W KL EI+L+++L NK +++E++FTYKM  +K+L++
Sbjct: 61  GTIVLNVTDNVLRQVINQPTAYALWNKLNEIYLNKDLSNKAFLRERFFTYKMDAAKSLTD 120

Query: 121 NLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVK 135
            L++FK+LSSEF+S+G  IG++NEAFI LNSLPE++K+ K
Sbjct: 121 ILNEFKRLSSEFRSIGYNIGEENEAFILLNSLPESFKDAK 160

BLAST of Moc05g28200 vs. NCBI nr
Match: XP_038885928.1 (uncharacterized protein LOC120076236 [Benincasa hispida])

HSP 1 Score: 149.1 bits (375), Expect = 2.9e-32
Identity = 73/160 (45.62%), Postives = 107/160 (66.88%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASKAILDQAN------------------ 60
           M   R +I+KF+ K DFELWK KIK +L + KA  AI D A                   
Sbjct: 1   MTTTRYEIEKFEAKTDFELWKAKIKVVLRKQKALLAITDPAKYPKILFKAEKETIESNAY 60

Query: 61  --------DNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSE 120
                   D+VLRQ+++  TAY +W KL +I+L+++LPNK +++E++FTYKM  +K+L++
Sbjct: 61  GTIVLNVIDSVLRQIVDRPTAYALWNKLNDIYLNKDLPNKAFLRERFFTYKMDPAKSLTD 120

Query: 121 NLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVK 135
           NL++FK LSS+F+S+G+ IG++NEAFI LNSLPE +K+VK
Sbjct: 121 NLNEFKSLSSDFRSIGDNIGEENEAFILLNSLPETFKDVK 160

BLAST of Moc05g28200 vs. NCBI nr
Match: XP_038880522.1 (uncharacterized protein LOC120072186 [Benincasa hispida])

HSP 1 Score: 148.7 bits (374), Expect = 3.7e-32
Identity = 74/160 (46.25%), Postives = 107/160 (66.88%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHK--------------------------AS 60
           MA  R +I+KFDGK +FELWK KIK +LGQ K                          A 
Sbjct: 1   MATTRFEIEKFDGKTNFELWKAKIKIVLGQQKELVAITNPTKYPKTLTDAEKETIEVNAH 60

Query: 61  KAILDQANDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSE 120
             I+    D+VLRQ+++ +T Y +W KL EI+L+++LPNK ++KE +FTYKM   K+L+E
Sbjct: 61  VTIVSNVTDSVLRQIVDQQTDYDLWNKLNEIYLNKDLPNKDFLKELFFTYKMDAVKSLTE 120

Query: 121 NLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVK 135
           NL++FK+LSSEF+S+G+ I ++NEAFI LNSLP+++K+V+
Sbjct: 121 NLNEFKRLSSEFRSIGDNIREENEAFILLNSLPDSFKDVQ 160

BLAST of Moc05g28200 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 3.7e-14
Identity = 45/162 (27.78%), Postives = 84/162 (51.85%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALL---GQHK-----------------------AS 60
           M+  + ++ KF+G   F  W+ +++ LL   G HK                       A+
Sbjct: 1   MSGVKYEVAKFNGDNGFSTWQRRMRDLLIQQGLHKVLDVDSKKPDTMKAEDWADLDERAA 60

Query: 61  KAILDQANDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSE 120
            AI    +D+V+  +++ +TA  IWT+LE +++S+ L NK+Y+K++ +   M        
Sbjct: 61  SAIRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLS 120

Query: 121 NLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVKLT 137
           +L+ F  L ++  +LG KI ++++A + LNSLP +Y  +  T
Sbjct: 121 HLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATT 162

BLAST of Moc05g28200 vs. ExPASy TrEMBL
Match: A0A2P5CTT8 (Uncharacterized protein OS=Trema orientale OX=63057 GN=TorRG33x02_273130 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.3e-30
Identity = 70/162 (43.21%), Postives = 106/162 (65.43%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASKAILDQAN------------------ 60
           M  ++I+I+KFDGKGDF +WK K+KA+L Q K +K + D +                   
Sbjct: 1   MGTSKIEIEKFDGKGDFNMWKKKMKAVLVQQKCAKVLGDASGLPETMKPSEKEELLETAY 60

Query: 61  --------DNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSE 120
                   DNVLRQV E +TA K+W+KL+ ++L++ L NK+Y+KE+ F +KM ++K+L +
Sbjct: 61  SLLILNLADNVLRQVDEQDTAAKVWSKLDSLYLTKTLSNKIYLKEQLFGFKMDSTKSLED 120

Query: 121 NLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVKLT 137
           NLDDFK+++    ++ EKI D+N+A I LNSLPE+YK++K T
Sbjct: 121 NLDDFKRITVSLANIDEKINDENQAIIILNSLPESYKDLKST 162

BLAST of Moc05g28200 vs. ExPASy TrEMBL
Match: A0A2P5FE65 (Uncharacterized protein (Fragment) OS=Trema orientale OX=63057 GN=TorRG33x02_080180 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.7e-30
Identity = 70/160 (43.75%), Postives = 106/160 (66.25%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASKAILDQAN------------------ 60
           M  ++I+I+KFDGKGDF +WK K+KA+L Q K +KA+ D +                   
Sbjct: 1   MGTSKIEIEKFDGKGDFNMWKKKMKAVLVQQKCAKALGDVSGLPETMKPSEKEELLETAY 60

Query: 61  --------DNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSE 120
                   DNVLRQV E +TA K+W+KL+ ++L++ L NK+Y+KE+ F +KM ++K+L +
Sbjct: 61  SLLILNLADNVLRQVDEQDTAAKVWSKLDSLYLTKTLSNKIYLKEQLFGFKMDSTKSLED 120

Query: 121 NLDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVK 135
           NLDDFK+++    ++ EKI D+N+A I LNSLPE+YK++K
Sbjct: 121 NLDDFKRITVSLANIDEKINDENQAIIILNSLPESYKDLK 160

BLAST of Moc05g28200 vs. ExPASy TrEMBL
Match: A0A5D3DNU1 (Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004440 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 6.4e-30
Identity = 72/159 (45.28%), Postives = 99/159 (62.26%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASK------------------------- 60
           MA  R ++ KF+G GDF LW+ KI+A+L QHK +K                         
Sbjct: 1   MASTRFEVSKFNGHGDFALWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYS 60

Query: 61  AILDQANDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSEN 120
            IL   +D VLR V E  T  ++W KLE ++L++ LPNK+YIKEK+F YKM  SK+L EN
Sbjct: 61  TILLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLPNKIYIKEKFFGYKMDQSKSLEEN 120

Query: 121 LDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVK 135
           LD+F+K+  +  ++GEK+ D+N+A I LNSLPE Y+EVK
Sbjct: 121 LDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVK 159

BLAST of Moc05g28200 vs. ExPASy TrEMBL
Match: A0A5A7U2U7 (Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00590 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-29
Identity = 72/159 (45.28%), Postives = 98/159 (61.64%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASK------------------------- 60
           MA  R ++ KF+G GDF LW+ KI+A+L QHK +K                         
Sbjct: 1   MASTRFEVSKFNGHGDFALWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYW 60

Query: 61  AILDQANDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSEN 120
            IL   +D VLR V E  T  ++W KLE ++L++ LPNK+YIKEK+F YKM  SK L EN
Sbjct: 61  TILLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLPNKIYIKEKFFGYKMDQSKILEEN 120

Query: 121 LDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVK 135
           LD+F+K+  +  ++GEK+ D+N+A I LNSLPE Y+EVK
Sbjct: 121 LDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVK 159

BLAST of Moc05g28200 vs. ExPASy TrEMBL
Match: A0A5A7UB25 (Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold560G00190 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 7.1e-29
Identity = 71/159 (44.65%), Postives = 98/159 (61.64%), Query Frame = 0

Query: 1   MAVARIDIDKFDGKGDFELWKVKIKALLGQHKASK------------------------- 60
           MA  R ++ KF+G GDF LW+ KI+A+L QHK +K                         
Sbjct: 1   MASTRFEVSKFNGHGDFSLWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYS 60

Query: 61  AILDQANDNVLRQVMEVETAYKIWTKLEEIFLSRELPNKVYIKEKYFTYKMGNSKTLSEN 120
            IL   +D VLR V E  T  ++W KLE ++L++ L NK+YIKEK+F YKM  SK+L EN
Sbjct: 61  TILLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLLNKIYIKEKFFGYKMDQSKSLEEN 120

Query: 121 LDDFKKLSSEFKSLGEKIGDDNEAFIPLNSLPEAYKEVK 135
           LD+F+K+  +  ++GEK+ D+N+A I LNSLPE Y+EVK
Sbjct: 121 LDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVK 159

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896323.14.3e-3652.08uncharacterized protein LOC120084587 [Benincasa hispida][more]
XP_038889933.16.8e-3446.91uncharacterized protein LOC120079695 [Benincasa hispida][more]
XP_038890043.18.9e-3449.38uncharacterized protein LOC120079747 [Benincasa hispida][more]
XP_038885928.12.9e-3245.63uncharacterized protein LOC120076236 [Benincasa hispida][more]
XP_038880522.13.7e-3246.25uncharacterized protein LOC120072186 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P109783.7e-1427.78Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
A0A2P5CTT81.3e-3043.21Uncharacterized protein OS=Trema orientale OX=63057 GN=TorRG33x02_273130 PE=4 SV... [more]
A0A2P5FE651.7e-3043.75Uncharacterized protein (Fragment) OS=Trema orientale OX=63057 GN=TorRG33x02_080... [more]
A0A5D3DNU16.4e-3045.28Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A5A7U2U71.1e-2945.28Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. maku... [more]
A0A5A7UB257.1e-2944.65Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 31..133
e-value: 6.7E-23
score: 81.0
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 10..132
NoneNo IPR availablePANTHERPTHR34676:SF12ZINC FINGER, CCHC-TYPE, RIBONUCLEASE H-LIKE DOMAIN, GAG-PRE-INTEGRASE DOMAIN PROTEIN-RELATEDcoord: 10..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g28200.1Moc05g28200.1mRNA