Moc05g27900 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g27900
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Pectin lyase-like superfamily protein
Location	chr5: 20420941 .. 20422897 (+)
RNA-Seq Expression	Moc05g27900
Synteny	Moc05g27900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTCATTTTTGCCTTTGTTTTCATCTTCTCCATTGATATTTTAAGCTCATGGAGTGTATTAGCTTCATCCAATTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGCCAAGCTGATGATTCACAAGTAAATTTAAACTACATTTTCATTTTGATTTTAATTTTTTTTTTTTTTGAATTTCTTGTAATTCTCTCTCTTCTTCCACTAATAAGTTTACTTTTTTCTTAGGCTTTTTTGAGAGCTTGGAATGATGTGTGTGGAGCAACAGAAGGAATTCCAACATTACATGCACCACTTGGAAAAATTTACATGTTGAGCCCTCTCAAATTTCAGGGCCCTTGCAAGTCCAAACAAATTATTTTCCAAGTAATTAAGATAATTTTCCATACCCCATTTCTTAATTTTATTTTGTCACATGTTTATTTTTTTTATTTCGAAAATATTTTGATATGGTTTTGTGAATTTGTAGCTTGGAGGGACTCTAATGGCTCCAACAAAGGGTTCATGGCTAAGTGACAAGACGGATAGATGGATTCAATTTTCAGACATAGATGGTCTCATGGTAAATGGAGGTGGTCAATTCGACGGTCAAGGTTCTTCCTGGTGGAGAGATTGTCAAGGAAATTGTCCAACGGTAAGATTTGATTTTTTTAGTACAACAATTTGAGGATATGGAAGATCGAACTTTCAACTTCGAGAGAAAGAACATATGTCCATTACCATTGAGCTGCATGCTCATGTTGGTCGTCACTGTAGGATTTGATTTTGATGTTTAAATTTAATGTTGATATTAATTATAATATATGGTTTTGCAGGCATTGTTTTTCCAAAATTGCAATGGGCTCCAACTAAGGGAAATGAAGCATGTCAATAGTGCCAAAAATCATATCTCCATAAATTCTTGTAACAATGCCATTATCTCAGACCTTCACATTATTGCTCCTAAAAATAGTCCAAACACAGATGGAATTGACATTACTGGATCCAACAATGTTCTTATTCAAAATTCTTTCATTGAAACTGGTAAGTAATTAACATTTCCCATTGATATATATATATATATATTTTTCTTTTGTTTGAGTAGTCAATTTTATTTCATATTAATTTCTATTCCTGTAGGTTATGATTGTATTGCCCTTAACAATGGATCTACCAATATCGATATCGTAGACGTGACCTGTGGGCTCGGCCATGGCATAAGGTAAAAATGACGCTTTTTCAATAACTCTTTATCGAAAATCTTTAGTCGTAATATACATCTTATTATCGTTATCGTTGTATTTGTTTTAAAACAGCATTGGAAGTTTAAGAGAAAATGGAGAATTCAACCTAATTGAAAACATACATGTAAGAAATTGCCTCTTCAAGGACACTCTATATGGAATGACAATCAAGACTTGGCAGGTTATAATTGTCTTTCAATTTAATTCATTTACAATAATAGTATTATATATATATATATATATATATAATTTATTTTTAACCTTTTGAATTATTTATTATAGGGCGGATATGGATACGTAAGAGACATTACATTTGAACAAATAACGCTTGTGGGTGTGAACATCCCGATCATTATCGATCGATATTACACTAATCTGGCAAGTATGAGGAAAAATAAGGTACAATTTTATCTACTTCTCATTTCTTATAGATTTTTTTTAAACCCATGTTGTGCTTGGTGTGTTGTGATAATTTATAAATTTTTTATTGATGCTTCCAGTTAATTGGTATCAAAGTGAGTGACGTGACGTTTCGTGAAGTTCATGGAACGTCGGCAAATGAGACTGTAATCATTCTGAACTGCTCTCGAAGTCGTTGCAGCAATATTACTATGGAAAATGTGAGCATGTGTACAACAATTCCAGGAAAAGAAGCCGAAGCTTTTTGTGAAAATGTTGATGGGAGGTTTACTAATGTAGTCCCGACCGTGTCTTGTTTATCAAACTAG

mRNA sequence

ATGAAGCTCATTTTTGCCTTTGTTTTCATCTTCTCCATTGATATTTTAAGCTCATGGAGTGTATTAGCTTCATCCAATTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGCCAAGCTGATGATTCACAACTTGGAGGGACTCTAATGGCTCCAACAAAGGGTTCATGGCTAAGTGACAAGACGGATAGATGGATTCAATTTTCAGACATAGATGGTCTCATGGTAAATGGAGGTGGTCAATTCGACGGTCAAGGTTCTTCCTGGTGGAGAGATTGTCAAGGAAATTGTCCAACGGCATTGTTTTTCCAAAATTGCAATGGGCTCCAACTAAGGGAAATGAAGCATGTCAATAGTGCCAAAAATCATATCTCCATAAATTCTTGTAACAATGCCATTATCTCAGACCTTCACATTATTGCTCCTAAAAATAGTCCAAACACAGATGGAATTGACATTACTGGATCCAACAATGTTCTTATTCAAAATTCTTTCATTGAAACTGGTTATGATTGTATTGCCCTTAACAATGGATCTACCAATATCGATATCGTAGACGTGACCTGTGGGCTCGGCCATGGCATAAGCATTGGAAGTTTAAGAGAAAATGGAGAATTCAACCTAATTGAAAACATACATGTAAGAAATTGCCTCTTCAAGGACACTCTATATGGAATGACAATCAAGACTTGGCAGTTAATTGGTATCAAAGTGAGTGACGTGACGTTTCGTGAAGTTCATGGAACGTCGGCAAATGAGACTGTAATCATTCTGAACTGCTCTCGAAGTCGTTGCAGCAATATTACTATGGAAAATGTGAGCATGTGTACAACAATTCCAGGAAAAGAAGCCGAAGCTTTTTGTGAAAATGTTGATGGGAGGTTTACTAATGTAGTCCCGACCGTGTCTTGTTTATCAAACTAG

Coding sequence (CDS)

ATGAAGCTCATTTTTGCCTTTGTTTTCATCTTCTCCATTGATATTTTAAGCTCATGGAGTGTATTAGCTTCATCCAATTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGCCAAGCTGATGATTCACAACTTGGAGGGACTCTAATGGCTCCAACAAAGGGTTCATGGCTAAGTGACAAGACGGATAGATGGATTCAATTTTCAGACATAGATGGTCTCATGGTAAATGGAGGTGGTCAATTCGACGGTCAAGGTTCTTCCTGGTGGAGAGATTGTCAAGGAAATTGTCCAACGGCATTGTTTTTCCAAAATTGCAATGGGCTCCAACTAAGGGAAATGAAGCATGTCAATAGTGCCAAAAATCATATCTCCATAAATTCTTGTAACAATGCCATTATCTCAGACCTTCACATTATTGCTCCTAAAAATAGTCCAAACACAGATGGAATTGACATTACTGGATCCAACAATGTTCTTATTCAAAATTCTTTCATTGAAACTGGTTATGATTGTATTGCCCTTAACAATGGATCTACCAATATCGATATCGTAGACGTGACCTGTGGGCTCGGCCATGGCATAAGCATTGGAAGTTTAAGAGAAAATGGAGAATTCAACCTAATTGAAAACATACATGTAAGAAATTGCCTCTTCAAGGACACTCTATATGGAATGACAATCAAGACTTGGCAGTTAATTGGTATCAAAGTGAGTGACGTGACGTTTCGTGAAGTTCATGGAACGTCGGCAAATGAGACTGTAATCATTCTGAACTGCTCTCGAAGTCGTTGCAGCAATATTACTATGGAAAATGTGAGCATGTGTACAACAATTCCAGGAAAAGAAGCCGAAGCTTTTTGTGAAAATGTTGATGGGAGGTTTACTAATGTAGTCCCGACCGTGTCTTGTTTATCAAACTAG

Protein sequence

MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDSQLGGTLMAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTWQLIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLSN

Homology

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BLAST of Moc05g27900 vs. NCBI nr
Match: XP_022154251.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 518.1 bits (1333), Expect = 5.3e-143
Identity = 258/297 (86.87%), Postives = 258/297 (86.87%), Query Frame = 0


Query: 50  MAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQNCNGL 109
           MAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQNCNGL
Sbjct: 1   MAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQNCNGL 60

Query: 110 QLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETG 169
           QLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETG
Sbjct: 61  QLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETG 120

Query: 170 YDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIK 229
           YDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIK
Sbjct: 121 YDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIK 180

Query: 230 TWQ---------------------------------------LIGIKVSDVTFREVHGTS 289
           TWQ                                       LIGIKVSDVTFREVHGTS
Sbjct: 181 TWQGGYGYVRDITFEQITLVGVNIPIIIDRYYTNLASMRKNKLIGIKVSDVTFREVHGTS 240

Query: 290 ANETVIILNCSRSRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLSN 308
           ANETVIILNCSRSRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLSN
Sbjct: 241 ANETVIILNCSRSRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLSN 297

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BLAST of Moc05g27900 vs. NCBI nr
Match: XP_022141108.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 434.5 bits (1116), Expect = 7.7e-118
Identity = 233/393 (59.29%), Postives = 263/393 (66.92%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDSQ---------------- 60
           MKL  A VFIFSI +LSSW V  S NFNVLDYGAIGNGQ DDSQ                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LGGTLMAPTKGSWLSDKTDRWIQFSDIDGL 120
                                         LGGT++APTK SW SD  DRWIQFSDIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 MVNGGGQFDGQGSSWWRDCQGNC--PTALFFQNCNGLQLREMKHVNSAKNHISINSCNNA 180
           MVNG GQFDGQGSSWW+DC  +C  PTALF  NCNGLQLREMKH+NS +NHISIN+CN+A
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLG 240
           IISDLHI+AP++SPNTDGIDI+GSNNVLIQNSF+ TG DCIALNNGSTNI+IV VTCG G
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTWQ-------------------- 300
           HGISIGSL ENG++N++E+IHV+NCL K T  G+ IKTWQ                    
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 -------------------LIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMENV 307
                              L+ IKVSDVT+REVHGTSANE  IILNCSRSRCSNI MENV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

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BLAST of Moc05g27900 vs. NCBI nr
Match: XP_022141107.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 414.1 bits (1063), Expect = 1.1e-111
Identity = 231/394 (58.63%), Postives = 258/394 (65.48%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDSQ---------------- 60
           MKL F  +FIFSI+ILSSW V AS +FNVLDYGAIGNGQADDSQ                
Sbjct: 1   MKLHFVLLFIFSINILSSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGI 60

Query: 61  ------------------------------LGGTLMAPTKGSWLSDKTDRWIQFSDIDGL 120
                                         L GTL+AP KGSW     +RWIQFSDIDGL
Sbjct: 61  PTLHAPHGKIYMLKPLKFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGL 120

Query: 121 MVNGGGQFDGQGSSWWRD---CQGNCPTALFFQNCNGLQLREMKHVNSAKNHISINSCNN 180
           +VNG GQFDGQGSSWW D   CQ   PTALF  NCNGLQLREMKH+NSAKNHISIN+CN+
Sbjct: 121 IVNGTGQFDGQGSSWWEDWEHCQR--PTALFLHNCNGLQLREMKHINSAKNHISINACND 180

Query: 181 AIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGL 240
            IISDLHIIAP++SPNTDGID++ SNNVLIQNSF+ TG DCIALNNGS+NIDIV VTCG 
Sbjct: 181 VIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGP 240

Query: 241 GHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTW-------------------- 300
           GHGISIGSL  N E+N++ENIHV+NCL K T  GM IKTW                    
Sbjct: 241 GHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGA 300

Query: 301 ------------------QLIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMENV 308
                             QL  IKVS+VT+REV+GTSANE  IILNCSR+RCSNI +ENV
Sbjct: 301 KNPIIIDQYYTDFFSIFFQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENV 360

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BLAST of Moc05g27900 vs. NCBI nr
Match: XP_038888688.1 (probable polygalacturonase At3g15720 [Benincasa hispida])

HSP 1 Score: 383.6 bits (984), Expect = 1.5e-102
Identity = 203/394 (51.52%), Postives = 252/394 (63.96%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSN-FNVLDYGAIGNGQADDS---------------- 60
           MKL+F FV IFSI  LSS +VLAS + FNVL YGA GN + DDS                
Sbjct: 1   MKLLFVFVSIFSIANLSSRNVLASPHYFNVLHYGATGNDETDDSQAFLKAWKDVCEATKG 60

Query: 61  ------------------------------QLGGTLMAPTKGSWLSDKTDRWIQFSDIDG 120
                                         QL GTL+AP KG+W +DK D+WI+F DIDG
Sbjct: 61  IPTLHVPLGKSYLLNPLTFQGPCKSKQVNIQLRGTLVAPKKGAWPTDKKDKWIEFHDIDG 120

Query: 121 LMVNGGGQFDGQGSSWWRDCQGNC--PTALFFQNCNGLQLREMKHVNSAKNHISINSCNN 180
           L +NGGGQ DGQGSSWW DC+ +C  PTALFF NCNG  LR+MKHVNS +NHISIN+CNN
Sbjct: 121 LTINGGGQIDGQGSSWWEDCETHCERPTALFFHNCNGFLLRDMKHVNSGRNHISINACNN 180

Query: 181 AIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGL 240
            ++S++HIIAP+ SPNTDGIDI  S NV IQ SF+ TG DC+A+NNGS+NI+IV +TCG 
Sbjct: 181 VMVSNIHIIAPEASPNTDGIDIARSTNVFIQKSFMATGDDCVAINNGSSNINIVGITCGP 240

Query: 241 GHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTWQ------------------- 300
           GHGISIGSL  +G++N++E++HVRNC FK    G+ IKTW+                   
Sbjct: 241 GHGISIGSLGADGQYNVVEDVHVRNCFFKGAQNGVRIKTWEGGYGYARNITFEKITLMNT 300

Query: 301 --------------------LIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMEN 307
                               +I IKVSDVT+REVHGTSANE  I LNCSR+RC+NI M+N
Sbjct: 301 RNPIIIDQYYTTSASSRKNKVINIKVSDVTYREVHGTSANENAITLNCSRARCTNILMDN 360

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BLAST of Moc05g27900 vs. NCBI nr
Match: KGN53196.1 (hypothetical protein Csa_014667 [Cucumis sativus])

HSP 1 Score: 374.4 bits (960), Expect = 9.4e-100
Identity = 198/390 (50.77%), Postives = 252/390 (64.62%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDS----------------- 60
           MKL+ A +F++SI ILSSW+VL SS+FNVLDYGAIGNG+ DDS                 
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  -------------------------QLGGTLMAPTKGSWLSDKTDRWIQFSDIDGLMVNG 120
                                    QLGGTLMAPTK  W+SD   +WIQF DI+GL + G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 121 GGQFDGQGSSWWRDC-QGNC--PTALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIIS 180
           GG+FDGQGS WW+DC   +C  PTALFF NCNGLQ++ MKH+NSAKNHISIN C++ I S
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 181 DLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGI 240
            LHI AP++SPNTDGIDI+ S N+ I++SF+ TG DCIA+NNGS+NI+I  +TCG GHGI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 241 SIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTWQ----------------------- 300
           SIGSL ++GE+N++EN+HV NCL + T  G+ IKTW+                       
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

Query: 301 ----------------LIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMENVSMC 307
                            + IKVSDVT+REV+GTSANE  I  NCS++RCSNI ++NV++ 
Sbjct: 301 IIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIK 360

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BLAST of Moc05g27900 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.1e-56
Identity = 126/306 (41.18%), Postives = 177/306 (57.84%), Query Frame = 0


Query: 44  QLGGTLMAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFF 103
           Q+ G L+AP+KG+W  DK D+WI F+DI+GL++ G G+ +GQGSSWW + +G+ PTAL F
Sbjct: 86  QMLGKLVAPSKGNWKGDK-DQWILFTDIEGLVIEGDGEINGQGSSWW-EHKGSRPTALKF 145

Query: 104 QNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQN 163
           ++CN L+L  + H++S   HI I+ CN   IS L I AP++SPNTDGID+  S+NV+IQ+
Sbjct: 146 RSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQD 205

Query: 164 SFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTL 223
             I TG DCIA+N+G++NI I  + CG GHGISIGSL ++GE   +EN+ V+NC F+ T+
Sbjct: 206 CIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTM 265

Query: 224 YGMTIKTWQ-----------------------------------------LIGIKVSDVT 283
            G  IKTWQ                                            ++VS V 
Sbjct: 266 NGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVV 325

Query: 284 FREVHGTSANETVIILNCS-RSRCSNITMENVSMCTTI--PGKEAEAFCENVDGRFTNVV 306
           F    GTS +E  +   CS R  C+ I + ++ + T     G+ A+  C NV G  T  V
Sbjct: 326 FSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAV 385

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BLAST of Moc05g27900 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.6e-41
Identity = 106/309 (34.30%), Postives = 163/309 (52.75%), Query Frame = 0


Query: 44  QLGGTLMAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQG----NCPT 103
           ++ G+++AP     ++  +  WI F D+  + +  GG  D QG+S W+ C+     NCPT
Sbjct: 95  RIAGSIVAPEDFRIIA-SSKHWIWFEDVTDVSIY-GGILDAQGTSLWK-CKNNGGHNCPT 154

Query: 104 ---ALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGS 163
              +L F   N +++  +  +NS K HI I++ NN  I  + + A +NSPNTDGI +  S
Sbjct: 155 GAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESS 214

Query: 164 NNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRN 223
           ++V I NS I TG DCI++  GSTN+ I  + CG GHGISIGSL    E   ++N+ V N
Sbjct: 215 HSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSN 274

Query: 224 CLFKDTLYGMTIKTW---------------------------------------QLIGIK 283
             F  T  G+ IKTW                                       Q  G+K
Sbjct: 275 VDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCLHKPCPKQESGVK 334

Query: 284 VSDVTFREVHGTSANETVIILNCSRSR-CSNITMENVSMCTTIPGKEAEAFCENVDGRFT 306
           VS+V + ++HGTS  E  ++L+CS+ + C+ I M++V++ +    + A+A C+N +G   
Sbjct: 335 VSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSV--HRPAQASCDNANGSAN 394

[Close]

BLAST of Moc05g27900 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.5e-39
Identity = 93/247 (37.65%), Postives = 136/247 (55.06%), Query Frame = 0


Query: 44  QLGGTLMAPTKGS-WLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWW-RDCQGN----- 103
           Q+ GT++AP     W      +W+ F  +  L V GGG  +G G  WW R C+ N     
Sbjct: 114 QISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPC 173

Query: 104 --CPTALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDIT 163
              PTAL F  C  +++  +  ++S + HI++ SC    IS L +IAP  SPNTDGI I+
Sbjct: 174 RGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHIS 233

Query: 164 GSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHV 223
            S  ++I N+ + TG DCI++   ST I I ++ CG GHGISIGSL ++  +  + +I V
Sbjct: 234 VSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITV 293

Query: 224 RNCLFKDTLYGMTIKTWQLIGIKVSDVTFREVHGTSANETVII--LNC-SRSRCSN---- 275
              +  DT  G+ IKTWQ     VS + FR +   + +  +II    C SR  C+N    
Sbjct: 294 DTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSA 353

[Close]

BLAST of Moc05g27900 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.3e-38
Identity = 96/297 (32.32%), Postives = 147/297 (49.49%), Query Frame = 0


Query: 44  QLGGTLMAPT-KGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWW-RDCQ------- 103
           ++ GT+ A + +  W+     RWI+F DI  L + GGG  +G G +WW   C+       
Sbjct: 143 KIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKRKKSLPC 202

Query: 104 GNCPTALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDIT 163
            + PTAL F++C  L + ++   +S K H+S + C + I S+L + AP++SPNTDGI IT
Sbjct: 203 KSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIHIT 262

Query: 164 GSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHV 223
           G+  + + NS I TG DCI++ +GS  +   ++TCG GHGISIGSL +      +  + V
Sbjct: 263 GTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGVLV 322

Query: 224 RNCLFKDTLYGMTIKTW---------------------------------------QLIG 283
                 DT  G+ IKTW                                       Q   
Sbjct: 323 DGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESA 382

Query: 284 IKVSDVTFREVHGTSANETVIILNCSRSR-CSNITMENVSMCTTIPGKEAEAFCENV 292
           +KVS+V +  + GTSA+E  +  +CS+S  C    + N+++     GKE    C N+
Sbjct: 383 VKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGN-GGKETTMSCSNI 438

[Close]

BLAST of Moc05g27900 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.1e-38
Identity = 105/306 (34.31%), Postives = 148/306 (48.37%), Query Frame = 0


Query: 56  SWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNC--------------PTAL 115
           SW +++   W+QF+ + G  + G G  DGQG  WW    G C              PTA+
Sbjct: 132 SWKNNRI--WLQFAKLTGFTLMGKGVIDGQGKQWW---AGQCKWVNGREICNDRDRPTAI 191

Query: 116 FFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLI 175
            F    GL ++ +K +NS + H+   +C    I  + I AP++SPNTDGIDI  S N  +
Sbjct: 192 KFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHL 251

Query: 176 QNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKD 235
           Q + I TG DC+A+  GS+NI I D+ CG GHGISIGSL        +  +HV    F D
Sbjct: 252 QKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFID 311

Query: 236 TLYGMTIKTWQ-----------------------LI----------------GIKVSDVT 295
           T  G+ IKTWQ                       LI                 +++ DVT
Sbjct: 312 TQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVT 371

Query: 296 FREVHGTSANETVIILNCSRSR-CSNITMENVSMCTTIPGKEAEAFCENVDGRFT-NVVP 307
           ++ + GTSA    I L CS S  C +I + ++S+  T  GK A    +N +G F+ +V+P
Sbjct: 372 YKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLT-SGKIASCLNDNANGYFSGHVIP 431

[Close]

BLAST of Moc05g27900 vs. ExPASy TrEMBL
Match: A0A6J1DN64 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111021549 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.5e-143
Identity = 258/297 (86.87%), Postives = 258/297 (86.87%), Query Frame = 0


Query: 50  MAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQNCNGL 109
           MAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQNCNGL
Sbjct: 1   MAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQNCNGL 60

Query: 110 QLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETG 169
           QLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETG
Sbjct: 61  QLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETG 120

Query: 170 YDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIK 229
           YDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIK
Sbjct: 121 YDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIK 180

Query: 230 TWQ---------------------------------------LIGIKVSDVTFREVHGTS 289
           TWQ                                       LIGIKVSDVTFREVHGTS
Sbjct: 181 TWQGGYGYVRDITFEQITLVGVNIPIIIDRYYTNLASMRKNKLIGIKVSDVTFREVHGTS 240

Query: 290 ANETVIILNCSRSRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLSN 308
           ANETVIILNCSRSRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLSN
Sbjct: 241 ANETVIILNCSRSRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLSN 297

[Close]

BLAST of Moc05g27900 vs. ExPASy TrEMBL
Match: A0A6J1CJJ6 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011582 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.7e-118
Identity = 233/393 (59.29%), Postives = 263/393 (66.92%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDSQ---------------- 60
           MKL  A VFIFSI +LSSW V  S NFNVLDYGAIGNGQ DDSQ                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LGGTLMAPTKGSWLSDKTDRWIQFSDIDGL 120
                                         LGGT++APTK SW SD  DRWIQFSDIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 MVNGGGQFDGQGSSWWRDCQGNC--PTALFFQNCNGLQLREMKHVNSAKNHISINSCNNA 180
           MVNG GQFDGQGSSWW+DC  +C  PTALF  NCNGLQLREMKH+NS +NHISIN+CN+A
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLG 240
           IISDLHI+AP++SPNTDGIDI+GSNNVLIQNSF+ TG DCIALNNGSTNI+IV VTCG G
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTWQ-------------------- 300
           HGISIGSL ENG++N++E+IHV+NCL K T  G+ IKTWQ                    
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 -------------------LIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMENV 307
                              L+ IKVSDVT+REVHGTSANE  IILNCSRSRCSNI MENV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

[Close]

BLAST of Moc05g27900 vs. ExPASy TrEMBL
Match: A0A6J1CIX8 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011581 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 5.2e-112
Identity = 231/394 (58.63%), Postives = 258/394 (65.48%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDSQ---------------- 60
           MKL F  +FIFSI+ILSSW V AS +FNVLDYGAIGNGQADDSQ                
Sbjct: 1   MKLHFVLLFIFSINILSSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGI 60

Query: 61  ------------------------------LGGTLMAPTKGSWLSDKTDRWIQFSDIDGL 120
                                         L GTL+AP KGSW     +RWIQFSDIDGL
Sbjct: 61  PTLHAPHGKIYMLKPLKFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGL 120

Query: 121 MVNGGGQFDGQGSSWWRD---CQGNCPTALFFQNCNGLQLREMKHVNSAKNHISINSCNN 180
           +VNG GQFDGQGSSWW D   CQ   PTALF  NCNGLQLREMKH+NSAKNHISIN+CN+
Sbjct: 121 IVNGTGQFDGQGSSWWEDWEHCQR--PTALFLHNCNGLQLREMKHINSAKNHISINACND 180

Query: 181 AIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGL 240
            IISDLHIIAP++SPNTDGID++ SNNVLIQNSF+ TG DCIALNNGS+NIDIV VTCG 
Sbjct: 181 VIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGP 240

Query: 241 GHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTW-------------------- 300
           GHGISIGSL  N E+N++ENIHV+NCL K T  GM IKTW                    
Sbjct: 241 GHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGA 300

Query: 301 ------------------QLIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMENV 308
                             QL  IKVS+VT+REV+GTSANE  IILNCSR+RCSNI +ENV
Sbjct: 301 KNPIIIDQYYTDFFSIFFQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENV 360

[Close]

BLAST of Moc05g27900 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.6e-100
Identity = 198/390 (50.77%), Postives = 252/390 (64.62%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDS----------------- 60
           MKL+ A +F++SI ILSSW+VL SS+FNVLDYGAIGNG+ DDS                 
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  -------------------------QLGGTLMAPTKGSWLSDKTDRWIQFSDIDGLMVNG 120
                                    QLGGTLMAPTK  W+SD   +WIQF DI+GL + G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 121 GGQFDGQGSSWWRDC-QGNC--PTALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIIS 180
           GG+FDGQGS WW+DC   +C  PTALFF NCNGLQ++ MKH+NSAKNHISIN C++ I S
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 181 DLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGI 240
            LHI AP++SPNTDGIDI+ S N+ I++SF+ TG DCIA+NNGS+NI+I  +TCG GHGI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 241 SIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTWQ----------------------- 300
           SIGSL ++GE+N++EN+HV NCL + T  G+ IKTW+                       
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

Query: 301 ----------------LIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMENVSMC 307
                            + IKVSDVT+REV+GTSANE  I  NCS++RCSNI ++NV++ 
Sbjct: 301 IIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIK 360

[Close]

BLAST of Moc05g27900 vs. ExPASy TrEMBL
Match: A0A5D3CYS8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00190 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.2e-97
Identity = 197/394 (50.00%), Postives = 248/394 (62.94%), Query Frame = 0


Query: 1   MKLIFAFVFIFSIDILSSWSVLASSNFNVLDYGAIGNGQADDSQ---------------- 60
           MKL+ A +  +SI ILSS +VL+SS+FNVLDYGAIGNG+ DDS+                
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  ------------------------------LGGTLMAPTKGSWLSDKTDRWIQFSDIDGL 120
                                         LGGTLMAPTK  W +D   +W+QF D++GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 MVNGGGQFDGQGSSWWRDC---QGNCPTALFFQNCNGLQLREMKHVNSAKNHISINSCNN 180
            V GGG+FDGQGS WW+ C   Q   PTALFF NCNGLQ+  MKHVNSAKNHISIN CN+
Sbjct: 121 NVQGGGRFDGQGSCWWKGCDHKQCKRPTALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 AIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGL 240
            I S LHI AP++SPNTDGIDI+ S N+ IQ+SFI TG DC+A+NNGS+NI+I  +TCG 
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTWQ------------------- 300
           GHGISIGSL ++GE+N++EN+HV NCL ++T  G+ IKTW+                   
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 --------------------LIGIKVSDVTFREVHGTSANETVIILNCSRSRCSNITMEN 307
                                + IKVSDVT+REV+GTSANE  I  NCS++RCSNI ++N
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

[Close]

BLAST of Moc05g27900 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 3.6e-57
Identity = 126/306 (41.18%), Postives = 177/306 (57.84%), Query Frame = 0


Query: 44  QLGGTLMAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFF 103
           Q+ G L+AP+KG+W  DK D+WI F+DI+GL++ G G+ +GQGSSWW + +G+ PTAL F
Sbjct: 86  QMLGKLVAPSKGNWKGDK-DQWILFTDIEGLVIEGDGEINGQGSSWW-EHKGSRPTALKF 145

Query: 104 QNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQN 163
           ++CN L+L  + H++S   HI I+ CN   IS L I AP++SPNTDGID+  S+NV+IQ+
Sbjct: 146 RSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQD 205

Query: 164 SFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTL 223
             I TG DCIA+N+G++NI I  + CG GHGISIGSL ++GE   +EN+ V+NC F+ T+
Sbjct: 206 CIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTM 265

Query: 224 YGMTIKTWQ-----------------------------------------LIGIKVSDVT 283
            G  IKTWQ                                            ++VS V 
Sbjct: 266 NGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVV 325

Query: 284 FREVHGTSANETVIILNCS-RSRCSNITMENVSMCTTI--PGKEAEAFCENVDGRFTNVV 306
           F    GTS +E  +   CS R  C+ I + ++ + T     G+ A+  C NV G  T  V
Sbjct: 326 FSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAV 385

[Close]

BLAST of Moc05g27900 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 211.8 bits (538), Expect = 7.5e-55
Identity = 124/306 (40.52%), Postives = 175/306 (57.19%), Query Frame = 0


Query: 44  QLGGTLMAPTKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFF 103
           Q+ G L+AP+KG+W  DK D+WI F+DI+GL++ G G+ +GQGSSWW + +G+ PT   F
Sbjct: 85  QMLGKLVAPSKGNWKGDK-DQWILFTDIEGLVIEGDGEINGQGSSWW-EHKGSRPT---F 144

Query: 104 QNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQN 163
           ++CN L+L  + H++S   HI I+ CN   IS L I AP++SPNTDGID+  S+NV+IQ+
Sbjct: 145 RSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQD 204

Query: 164 SFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTL 223
             I TG DCIA+N+G++NI I  + CG GHGISIGSL ++GE   +EN+ V+NC F+ T+
Sbjct: 205 CIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTM 264

Query: 224 YGMTIKTWQ-----------------------------------------LIGIKVSDVT 283
            G  IKTWQ                                            ++VS V 
Sbjct: 265 NGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVV 324

Query: 284 FREVHGTSANETVIILNCS-RSRCSNITMENVSMCTTI--PGKEAEAFCENVDGRFTNVV 306
           F    GTS +E  +   CS R  C+ I + ++ + T     G+ A+  C NV G  T  V
Sbjct: 325 FSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAV 384

[Close]

BLAST of Moc05g27900 vs. TAIR 10
Match: AT5G44830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 5.6e-50
Identity = 106/301 (35.22%), Postives = 163/301 (54.15%), Query Frame = 0


Query: 47  GTLMAP-TKGSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPTALFFQN 106
           G ++AP  K +W   K  RW+ F +I GL VNG G   G+GSS+W+         L FQ 
Sbjct: 41  GKIVAPINKAAWSESKLFRWVSFKEIIGLTVNGSGTIHGRGSSFWKQ--------LHFQR 100

Query: 107 CNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVLIQNSF 166
           CN L++  +   NS +NHISI+ C    ++ + ++AP++SPNTDGI+I+GS++V + ++F
Sbjct: 101 CNDLKIIGITSFNSPRNHISISECKRVQLTKIKLVAPEDSPNTDGINISGSSDVDVYDTF 160

Query: 167 IETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFKDTLYG 226
           I TG DC+A+NNGS NI+I  + CG GHGIS+GSL  +GE +++EN+ V NC F  T  G
Sbjct: 161 IGTGDDCVAINNGSVNINITRMNCGPGHGISVGSLGRDGEESIVENVQVTNCTFFRTDNG 220

Query: 227 MTIKTW--------------------------------------QLIGIKVSDVTFREVH 286
           + IKTW                                      +   + +S+VTF ++ 
Sbjct: 221 VRIKTWPNGKGYARNILFKDLTFRESKNPIIIDQNYVDKGRLDVEESAVAISNVTFTDIR 280

Query: 287 GTSANETVIILNCSR-SRCSNITMENVSMCTTIPGKEAEAFCENVDGRFTNVVPTVSCLS 308
           GTS    +I ++CS  + C +I ++ + +  T+ G +    C NV G+  N      C  
Sbjct: 281 GTSQRNEIIKIDCSEVTYCKDIVLDKIDI-ATVDGNKPVVECSNVYGKSINTNDANGCFE 332

[Close]

BLAST of Moc05g27900 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.1e-48
Identity = 121/354 (34.18%), Postives = 177/354 (50.00%), Query Frame = 0


Query: 27  FNVLDYGAIGNGQADDS------------------------------------------- 86
           +NVL +GA G+GQ DDS                                           
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 87  ---QLGGTLMAPTK-GSWLSDKTDRWIQFSDIDGLMVNGGGQFDGQGSSWWRDCQGNCPT 146
              QL G ++AP+   SW + K+  WI FS + GLM++G G  +G+GSS+W         
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWE-------- 172

Query: 147 ALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNV 206
           AL   NCN L +  +  ++S K+HISI +C+   IS ++I+AP+NSPNTDGIDI+ S NV
Sbjct: 173 ALHICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYSTNV 232

Query: 207 LIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLF 266
            I +S I+TG DCIA+N GS++I+I  V CG GHGIS+GSL  +GE   + +++V  C F
Sbjct: 233 NIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQCTF 292

Query: 267 KDTLYGMTIKTWQ---------------LIGIK------------------------VSD 294
             T  G  IKTWQ               LI ++                        +S 
Sbjct: 293 NKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVAISS 352

[Close]

BLAST of Moc05g27900 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.0e-48
Identity = 122/388 (31.44%), Postives = 185/388 (47.68%), Query Frame = 0


Query: 10  IFSIDILSS---WSVLASSNFNVLDYGAIGNGQADDS----------------------- 69
           I  I IL +   + ++   N+NVL++ A G+GQ DDS                       
Sbjct: 4   ILDISILINFLCFGIVYGKNYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIP 63

Query: 70  -----------------------QLGGTLMAPT-KGSWLSDKTDRWIQFSDIDGLMVNGG 129
                                  QL GT++AP+ K +W    +  WI+FS + GL++ G 
Sbjct: 64  SGKTFLLQPTVFQGPCKSSSIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGS 123

Query: 130 GQFDGQGSSWW--RDCQGNCPTALFFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDL 189
           G  D +GSS+W         PTAL    C+ L++  +  ++S KNHISI +CN   IS++
Sbjct: 124 GTIDSRGSSFWELNLKASQRPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNI 183

Query: 190 HIIAPKNSPNTDGIDITGSNNVLIQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISI 249
           ++ AP+ SPNTDGIDI+ S N+ I +S I+TG DCIA+N+GS+NI+I  + CG GHGIS+
Sbjct: 184 NLFAPETSPNTDGIDISDSTNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISV 243

Query: 250 GSLRENGEFNLIENIHVRNCLFKDTLYGMTIKTW-------------------------- 308
           GSL   G    + ++ V +C F  T  G  IKTW                          
Sbjct: 244 GSLGAGGAEAKVSDVQVTHCTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIII 303

The following BLAST results are available for this feature:

BLAST of Moc05g27900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154251.1	5.3e-143	86.87	probable polygalacturonase At3g15720 [Momordica charantia]	[more]
XP_022141108.1	7.7e-118	59.29	probable polygalacturonase At3g15720 [Momordica charantia]	[more]
XP_022141107.1	1.1e-111	58.63	probable polygalacturonase At3g15720 [Momordica charantia]	[more]
XP_038888688.1	1.5e-102	51.52	probable polygalacturonase At3g15720 [Benincasa hispida]	[more]
KGN53196.1	9.4e-100	50.77	hypothetical protein Csa_014667 [Cucumis sativus]	[more]

BLAST of Moc05g27900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LW07	5.1e-56	41.18	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
O22818	4.6e-41	34.30	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
Q94AJ5	2.5e-39	37.65	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q02096	1.3e-38	32.32	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
P43212	8.1e-38	34.31	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]

BLAST of Moc05g27900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DN64	2.5e-143	86.87	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1CJJ6	3.7e-118	59.29	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1CIX8	5.2e-112	58.63	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A0A0KZ95	4.6e-100	50.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1	[more]
A0A5D3CYS8	1.2e-97	50.00	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Moc05g27900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15720.1	3.6e-57	41.18	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	7.5e-55	40.52	Pectin lyase-like superfamily protein	[more]
AT5G44830.1	5.6e-50	35.22	Pectin lyase-like superfamily protein	[more]
AT5G27530.1	3.1e-48	34.18	Pectin lyase-like superfamily protein	[more]
AT4G35670.1	4.0e-48	31.44	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 209..230 e-value: 510.0 score: 9.3 coord: 156..177 e-value: 740.0 score: 8.2 coord: 179..199 e-value: 6800.0 score: 1.3 coord: 129..155 e-value: 36.0 score: 13.3
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 47..232 e-value: 1.8E-47 score: 162.0 coord: 235..292 e-value: 4.0E-9 score: 36.0
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 186..199
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 17..304 e-value: 3.7E-70 score: 238.9
None	No IPR available	PANTHER	PTHR31375:SF232	SUBFAMILY NOT NAMED	coord: 235..305
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 44..232
None	No IPR available	PANTHER	PTHR31375:SF232	SUBFAMILY NOT NAMED	coord: 44..232
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 235..305
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 15..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g27900.1	Moc05g27900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity