Moc05g27520 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g27520
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytoplasmic tRNA 2-thiolation protein 2
Location	chr5: 19887242 .. 19890723 (+)
RNA-Seq Expression	Moc05g27520
Synteny	Moc05g27520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTGCAATTCCTCAACCTGCCAATCCAACTGCTACAGAAATCAAGATGACGAAGAAACACTTGAACAACATTCGCCTCCAGCAAACAAGGCCGAGGCAGCCGCCGCCATGGAGTCCTGTCCAGTTGACACGAACGGCGACGGCAACACGCATCTCTGCTTCAAATGCAAGTCCAACGAGCGCATGTCGAGCTCTGGGGTAGGAGACGATGGCCGATTTTGCAGCGGTTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCTGGCCGTAACCTCGAACGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCCTTCTCTGGGGGTCCTTGTTCCAGGTTCTTCCTCTTTTCGAGACTTGTTTTTGTAAAGAATTGGTTAATGATTGTAGGTGCTAAGAGAATTAGTTTCTGGACCTGCGAACGGAAGGAATAGTTTTTCAGGCTGTACTTTTGATTAGAATTGAGAGTGCCGATAAATTAATCGTGTTTTCCTGTTATTTGTATGCCTTCGTTCCATTCCAACGACTGGTTAATATCTTGATAGTTCTTAGTTATTTATAATCTGGTCTTCTTTTAGATAATCACCTATACATTTATTTATTCCGGATTCTTTTATTACCAGTCTATCTTGAATGCTCTGGAAACTGCTGCACGCGATCTAAGAGACTTACCCCCTCACTTCCCTCATTCCATTAAAACTTTCAGGGTGGCTTTGCAATTTGTCCATGAGTTGCAAGTGAAAGCACACAAAAATTTTGAAGCGAGTAGGGATAGATCATTACAAGTTTTTGGTGTTGGAGTTGCTTATATCGACGAAACATCTGCTTGTTCTATCCCTTCACACGAGATGGACAGTGCAATTGAAACCATCCAATCAGTTGTGTCAAATCTACGGCCACCAAGTAAAGAGTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGGGTGATAGAAGGGAGAAGTTGAAGAAGTTGCTAGATACTGTCAATGATGTCACAGGAAAGGAAGACCTTCTCTCACACTTGCGGATGTTATCCTTGCAAAAAGTTTGCCCCTCAATTTACTCCCTTATTGGCTTGCAATCAAAGAGAGGGAATTTCTCAGTCCTATTGTTTGTTTTGCAGATTGCTACTGAGAATGGATATAATAGACTTCTAGTCGGGACATGTACCTCAAGGATTGCTCGGCACGTGATTTCTTCCACTGTTAAGGTTTGCCTTCAAATGAGCTCATATTAGGTTTTTGTTTTTAAGCATTTAATTTGAATGTTTAATCACTAGAGTTTTCAGGGCCACGATATTGACCGACCAAAATAAATGTTAGAAGGTTGGGTACATTGACAGTTATCTGAGACGGAACAGGACAATTGGTCCATTGTTCAAGCTTAGATCTTCATGATATTGTACAAGGATCCAAATTGGAACAACTAATATGATTTGCTAGCTCAATGGCCAAGACATCTATATTTTTTTCAAGAGGTCATAGATTTGAGCAAAAAAAAAAAAGAATAATTAGAAAAAGAGGTGCACTTTTGGTTTCAAACGCTCGGAAAATGCTGCATCAATTGTGAGCAATTGATGTTATTTCACTTTAAAAACTACTGCACACTTAATAATGTGGAGATCTCTGACAAAAAAAAAGGGTTAGAGAGGGTGTTGTGTTACTTTGAATAAAATAACTATAGAAAAGTACCGTGTTCATAGGATCATTAACTATGAGCTTGGCAATTGTACTCTTTTAATTATAATTTAGGCAAAGGCATGGCTAGCACTACACTCTGCAGGAAACACTGGTGATGCTTGGGGCCATTATTACCTTTCAGGAAACACCCCTATTAAGATATAGAAAATAGCCACGGTAGAGCAATGGAATCTTTGTATTACTATTATTCTGTTGTCCGAAATTTTATTGCTTATTGTTGTTAGTTGTTACAAACGGCTTATTTTGTTCTATTTTCTAAAATATTCCCATCTGGGATTGGTCTATGTACCGGTTATGACTTTATTATTTTAATCAAGGCAGGGCCAAGGGTATTCCTTGCCGGCAGATATACAGTATGTTGATGCAAGGTGGGAGGTCCCAGTGGCACTTCCTCTACGTGACTGTATTGCACAAGAGCTCCACATGCTCTGCTGTGTTGATGGGTTTGTTAATTTTGTATACTGTTCTGTTTATCATCAGGATCAATTCTTGGTCACCTTTGTCCTGTTTAGTTCTTGACTGCTGTTAAATTAACTTGCGGTCAATTGCATTTCTAATTTGTACGCTTCGACTGAATCTAACTAATGATGAGAGTTGTTCTTGGCAGGGCAGTCTGAAGACAGTGGAACTGGCCAAAAATTCCTTCTCTGGCATCAATGGCTTGGTGTCATCATTTGTAGCTCTATTGCAGGTACAAGTAGAAAGTGGAATTAGAAAAATATGCAATTTCATGCATTTTGGCGTAGGAGACACGACAGTTGGGAAGGGCAACTAATCTGTAACTGTAAATCACTGCTGTGCAGCAAAAGCACATGTTTTTGTGTTCAAATTAGGAGCAATTTTGGTTGGGCATGGAAACAATTTTCCTTCTAATTGAACTCCTTGAATTTCACCTTCAAATTTAAGTGATTGTTTTTTAGTAAGATAAGATACAAAATGCACATACACCTTCACATTGTAGGCCACTTGCCCTCCCAGGAATGTTATGCCGAACCTGACCGAAGAAATTTTGTTCTACTTTCTTTTCTAGGAAGAGAATCCATCTCGTGAGTCTACAATTGTGAGAACTGCTGGCAAGCTTACTCCGTTCAATTTTAGCAGGATTCCAGATTTGGATGAAAGTAATAATGTTCCTTTGGCCACCAAAAGGCGTCAAAAGAGAAATAACGTTAAGCATGTTAACTCCATATCGTCAGAGTCATTTTGTGCAGTCTGCAATAGCCCACTTAACATATCTGATGTGCAAACTTTAAGAAGTTGTGAAACTAGTCCGGAGACTGCTAATATTTTGAGTGGTACCTGGTGTTCGAGTTGTCAATTTCAGATTCTTCCTAAGGATTCCACGTCACAAGAACTTTTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCAATTCACGGCAGCACTGGAAATTCCAATTCGCTAAGGTAAGAGTAAGTATTGTGTTACGGTTATTTTGCAAAAAAAGGTCTTGTATTGCAGTTGGCACCATGAAGGAAAAGGAGTGTAAATATAACATTTAATAACTTCCAGCCAGTGTCTGTCTGTTTCTTATAATGTCTTATTAACTATTAAGTCATGTTGGGTGGACTGGATGAAGTAATTGAAAATGAAATAGCGTTGTTTATGAACTGTGCTTCATTTATTACTACATTGAGCTTCTTAAGTATTACTTTGAAAATTTTCAGGCAAAATATTTCTGTTATTAATTGCAGGGAGCAAATACAGGAGTTCTTGCTCTCAGATGATGAAGCCGAATCATAA

mRNA sequence

ATGGCGTGCAATTCCTCAACCTGCCAATCCAACTGCTACAGAAATCAAGATGACGAAGAAACACTTGAACAACATTCGCCTCCAGCAAACAAGGCCGAGGCAGCCGCCGCCATGGAGTCCTGTCCAGTTGACACGAACGGCGACGGCAACACGCATCTCTGCTTCAAATGCAAGTCCAACGAGCGCATGTCGAGCTCTGGGGTAGGAGACGATGGCCGATTTTGCAGCGGTTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCTGGCCGTAACCTCGAACGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCCTTCTCTGGGGGTCCTTGTTCCAGGGTGGCTTTGCAATTTGTCCATGAGTTGCAAGTGAAAGCACACAAAAATTTTGAAGCGAGTAGGGATAGATCATTACAAGTTTTTGGTGTTGGAGTTGCTTATATCGACGAAACATCTGCTTGTTCTATCCCTTCACACGAGATGGACAGTGCAATTGAAACCATCCAATCAGTTGTGTCAAATCTACGGCCACCAAGTAAAGAGTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGGGTGATAGAAGGGAGAAGTTGAAGAAGTTGCTAGATACTGTCAATGATGTCACAGGAAAGGAAGACCTTCTCTCACACTTGCGGATGTTATCCTTGCAAAAAATTGCTACTGAGAATGGATATAATAGACTTCTAGTCGGGACATGTACCTCAAGGATTGCTCGGCACGTGATTTCTTCCACTGTTAAGGGCCAAGGGTATTCCTTGCCGGCAGATATACAGTATGTTGATGCAAGGTGGGAGGTCCCAGTGGCACTTCCTCTACGTGACTGTATTGCACAAGAGCTCCACATGCTCTGCTGTGTTGATGGTCTGAAGACAGTGGAACTGGCCAAAAATTCCTTCTCTGGCATCAATGGCTTGGTGTCATCATTTGTAGCTCTATTGCAGGAAGAGAATCCATCTCGTGAGTCTACAATTGTGAGAACTGCTGGCAAGCTTACTCCGTTCAATTTTAGCAGGATTCCAGATTTGGATGAAAGTAATAATGTTCCTTTGGCCACCAAAAGGCGTCAAAAGAGAAATAACGTTAAGCATGTTAACTCCATATCGTCAGAGTCATTTTGTGCAGTCTGCAATAGCCCACTTAACATATCTGATGTGCAAACTTTAAGAAGTTGTGAAACTAGTCCGGAGACTGCTAATATTTTGAGTGGTACCTGGTGTTCGAGTTGTCAATTTCAGATTCTTCCTAAGGATTCCACGTCACAAGAACTTTTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCAATTCACGGCAGCACTGGAAATTCCAATTCGCTAAGGGAGCAAATACAGGAGTTCTTGCTCTCAGATGATGAAGCCGAATCATAA

Coding sequence (CDS)

ATGGCGTGCAATTCCTCAACCTGCCAATCCAACTGCTACAGAAATCAAGATGACGAAGAAACACTTGAACAACATTCGCCTCCAGCAAACAAGGCCGAGGCAGCCGCCGCCATGGAGTCCTGTCCAGTTGACACGAACGGCGACGGCAACACGCATCTCTGCTTCAAATGCAAGTCCAACGAGCGCATGTCGAGCTCTGGGGTAGGAGACGATGGCCGATTTTGCAGCGGTTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCTGGCCGTAACCTCGAACGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCCTTCTCTGGGGGTCCTTGTTCCAGGGTGGCTTTGCAATTTGTCCATGAGTTGCAAGTGAAAGCACACAAAAATTTTGAAGCGAGTAGGGATAGATCATTACAAGTTTTTGGTGTTGGAGTTGCTTATATCGACGAAACATCTGCTTGTTCTATCCCTTCACACGAGATGGACAGTGCAATTGAAACCATCCAATCAGTTGTGTCAAATCTACGGCCACCAAGTAAAGAGTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGGGTGATAGAAGGGAGAAGTTGAAGAAGTTGCTAGATACTGTCAATGATGTCACAGGAAAGGAAGACCTTCTCTCACACTTGCGGATGTTATCCTTGCAAAAAATTGCTACTGAGAATGGATATAATAGACTTCTAGTCGGGACATGTACCTCAAGGATTGCTCGGCACGTGATTTCTTCCACTGTTAAGGGCCAAGGGTATTCCTTGCCGGCAGATATACAGTATGTTGATGCAAGGTGGGAGGTCCCAGTGGCACTTCCTCTACGTGACTGTATTGCACAAGAGCTCCACATGCTCTGCTGTGTTGATGGTCTGAAGACAGTGGAACTGGCCAAAAATTCCTTCTCTGGCATCAATGGCTTGGTGTCATCATTTGTAGCTCTATTGCAGGAAGAGAATCCATCTCGTGAGTCTACAATTGTGAGAACTGCTGGCAAGCTTACTCCGTTCAATTTTAGCAGGATTCCAGATTTGGATGAAAGTAATAATGTTCCTTTGGCCACCAAAAGGCGTCAAAAGAGAAATAACGTTAAGCATGTTAACTCCATATCGTCAGAGTCATTTTGTGCAGTCTGCAATAGCCCACTTAACATATCTGATGTGCAAACTTTAAGAAGTTGTGAAACTAGTCCGGAGACTGCTAATATTTTGAGTGGTACCTGGTGTTCGAGTTGTCAATTTCAGATTCTTCCTAAGGATTCCACGTCACAAGAACTTTTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCAATTCACGGCAGCACTGGAAATTCCAATTCGCTAAGGGAGCAAATACAGGAGTTCTTGCTCTCAGATGATGAAGCCGAATCATAA

Protein sequence

MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSNERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPSKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES

Homology

[Close]

BLAST of Moc05g27520 vs. NCBI nr
Match: XP_022152059.1 (cytoplasmic tRNA 2-thiolation protein 2 [Momordica charantia])

HSP 1 Score: 938.7 bits (2425), Expect = 1.9e-269
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60

Query: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120
           ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV
Sbjct: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120

Query: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180
           HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS
Sbjct: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180

Query: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRL 240
           KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRL
Sbjct: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRL 240

Query: 241 LVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDG 300
           LVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDG
Sbjct: 241 LVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDG 300

Query: 301 LKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNV 360
           LKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNV
Sbjct: 301 LKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNV 360

Query: 361 PLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSS 420
           PLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSS
Sbjct: 361 PLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSS 420

Query: 421 CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES
Sbjct: 421 CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 475

[Close]

BLAST of Moc05g27520 vs. NCBI nr
Match: XP_038905927.1 (cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 398/474 (83.97%), Postives = 428/474 (90.30%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQ DE T+EQ SPP+++++ AAA+ESC VDTN D  +THLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQVSPPSSRSDTAAAVESCQVDTNDDSSHTHLCFKCKS 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ER+SSSGVGDDGR CS CFRSNLF KFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERISSSGVGDDGRLCSDCFRSNLFGKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEAS+DRSLQVFGVGVAYI+ET+A SIPSHE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASKDRSLQVFGVGVAYINETAAYSIPSHEIDQTIQTIQSVVSSLCPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
            K+LHIVQIE+VYSSNL D REKLKKLLDTVNDVTGKEDL+S+LRMLSLQKIA ENGYNR
Sbjct: 181 RKDLHIVQIENVYSSNLSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           LLVGTCTSRIA HVISSTVKGQGYSLPADIQY+DARWE+PV LPL DC+AQEL++LCCVD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADIQYIDARWEIPVVLPLLDCLAQELNLLCCVD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKTVEL K S SGIN LVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDL ES+N
Sbjct: 301 GLKTVELTKQSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLTESSN 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VPLATKRRQKRNN KHVNSISSESFCAVCN PLN SDVQTLR  E    +++IL  T CS
Sbjct: 361 VPLATKRRQKRNNFKHVNSISSESFCAVCNGPLNTSDVQTLRRFEAGQTSSSILGCTCCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEA 474
           SCQFQILPKDS SQELFLSLLPPSMVARA+HGS GNSNSLREQIQEFLLSDDEA
Sbjct: 421 SCQFQILPKDSLSQELFLSLLPPSMVARAMHGSFGNSNSLREQIQEFLLSDDEA 474

[Close]

BLAST of Moc05g27520 vs. NCBI nr
Match: XP_023539180.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.8 bits (2002), Expect = 2.2e-220
Identity = 402/476 (84.45%), Postives = 426/476 (89.50%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSSTCQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP HE+D  I+ IQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQAIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQKIA ENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           L++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC+AQEL+MLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKTVEL K SFSGIN LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDL+ESN 
Sbjct: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN+SDVQTLRS E   + ++IL+GT CS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAG-QVSSILAGTCCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           SCQFQILPKDS SQEL LSLLPPSMVARA+HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 421 SCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of Moc05g27520 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 401/476 (84.24%), Postives = 425/476 (89.29%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQKIA ENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           L++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC+AQEL+MLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKT EL K SFSGIN LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDL+ESN 
Sbjct: 301 GLKTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN+SDVQTLRS E   + ++IL+GT CS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAG-QVSSILAGTCCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           SCQFQILPKDS SQEL LSLLPPSMVARA+HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 421 SCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of Moc05g27520 vs. NCBI nr
Match: XP_022971223.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima])

HSP 1 Score: 767.7 bits (1981), Expect = 5.9e-218
Identity = 398/476 (83.61%), Postives = 423/476 (88.87%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NK +  AA       T GD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSL VFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQKIA ENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           L++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC+AQEL+MLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKTVEL K SFSGIN LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDL+ESN 
Sbjct: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN+SDVQTLRS E   + ++IL+GT CS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAG-QVSSILAGTCCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           SCQFQILPKDS SQEL LSLLPPSMVARA+HGS  NSNSLRE+IQEFLLSDDE ES
Sbjct: 421 SCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

[Close]

BLAST of Moc05g27520 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 510.8 bits (1314), Expect = 1.7e-143
Identity = 274/473 (57.93%), Postives = 346/473 (73.15%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSS C+S CY  + D           +K    A        + G  +  +C KCK N
Sbjct: 1   MACNSSGCESGCYDREKDN---------GSKIVDDAV-------SGGGNHESVCVKCKCN 60

Query: 61  ERMS-SSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
             M+   G  DDGRFC+ CFR+N+F KFRLAVTS+AMI+PSD VLVAFSGG  SRV+LQF
Sbjct: 61  APMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHELQ+KA KN+EASRDRSL VFGVGVA++DET+A    S EM  AIE ++  VS L PP
Sbjct: 121 VHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
           +K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDLL HL+MLSLQK+A ENGYNR
Sbjct: 181 AKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           L++G+CTSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLRDC+  E+  LC +D
Sbjct: 241 LVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKTVELA  S  GIN LVSSFVALLQEENPSRE TIVRTA KLTPF F++IP+ D+S N
Sbjct: 301 GLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS-N 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VP+AT+RR KR N+K+  S+++E+FC +CN PLN SD   L + E   E +++L    CS
Sbjct: 361 VPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQE-SDVLYAACCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDE 473
           SC+FQILP+D +S E F S LP  M+++  H    +   LRE+I++ LL DDE
Sbjct: 421 SCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

[Close]

BLAST of Moc05g27520 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.7e-103
Identity = 208/446 (46.64%), Postives = 290/446 (65.02%), Query Frame = 0


Query: 29  ANKAEAAAAMESCPVDTNGDGNTHLCFKC-KSNERMSSSGVGDDGRFCSGCFRSNLFAKF 88
           ++ A AAA  ++        G   L  +C K     ++  V      C  CFR+NL  KF
Sbjct: 19  SSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLLGKF 78

Query: 89  RLAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHELQVKAHKNFEASRDRSLQVFGVGVA 148
           +LAVTSNAM+ P+D VL+AFSGGP SRVALQF+HE++ KA ++++ S  ++L VFGVGVA
Sbjct: 79  KLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVSNSQALPVFGVGVA 138

Query: 149 YIDETSACSIPSHEMDSAIETIQSVVSNLRPPSKELHIVQIESVYSSNLGDRREKLKKLL 208
           ++DE+  CS P  E++ AIE I+S+VS+L    K +HIV++E V+S+   D   +L++ +
Sbjct: 139 FVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIVRLEDVFSTESEDGERRLREAV 198

Query: 209 DTVNDVTGKEDLLSHLRMLSLQKIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPA 268
           D + D TG+ED L  LRMLSLQKIA ENGY ++++G+C S IA HV+S+TVKGQGYSLPA
Sbjct: 199 DMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCASAIACHVLSATVKGQGYSLPA 258

Query: 269 DIQYVDARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQE 328
           D+QYVD RWE+PV LPLRDC+AQEL +LC +D LKT +      +GIN LV+SF+  L+E
Sbjct: 259 DVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHLDRPSNGINSLVASFIKRLRE 318

Query: 329 ENPSRESTIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAV 388
           ENPSRE TIVRTA KL PF+F++       + +P  ++ R K   V    S  SE  C +
Sbjct: 319 ENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLRPKFQKVDSDESTFSEILCLM 378

Query: 389 CNSPLNISDVQTLRSCE-TSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVA 448
           C SP + S++Q L S +  + +  ++ +   C SC FQILP      E F SLLP     
Sbjct: 379 CGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQILPAGENLNEHFFSLLPKLWTG 438

Query: 449 RAIHGSTGNSNSLREQIQEFLLSDDE 473
           +    S  +S  LR+QI+E+LL +++
Sbjct: 439 KMDTISDSHS-LLRDQIEEYLLEEND 461

[Close]

BLAST of Moc05g27520 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.9e-102
Identity = 206/446 (46.19%), Postives = 288/446 (64.57%), Query Frame = 0


Query: 29  ANKAEAAAAMESCPVDTNGDGNTHLCFKC-KSNERMSSSGVGDDGRFCSGCFRSNLFAKF 88
           ++ A AAA  ++        G   L  +C K     ++  V      C  CFR+NL  KF
Sbjct: 19  SSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLLGKF 78

Query: 89  RLAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHELQVKAHKNFEASRDRSLQVFGVGVA 148
           +LAVTSNAM+ P+D VL+AFSGGP SRVALQF+HE++ KA ++++ S  ++L VFGVGVA
Sbjct: 79  KLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVSNSQALPVFGVGVA 138

Query: 149 YIDETSACSIPSHEMDSAIETIQSVVSNLRPPSKELHIVQIESVYSSNLGDRREKLKKLL 208
           ++DE+  CS P  E++ AIE I+S+VS+L    K +HI ++E V+S+   D   +L++ +
Sbjct: 139 FVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIARLEDVFSTESEDGERRLREAV 198

Query: 209 DTVNDVTGKEDLLSHLRMLSLQKIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPA 268
           D + D TG+ED L  LRMLSLQKIA ENGY ++++G+C S IA HV+S+TVKGQGYSLPA
Sbjct: 199 DMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCASAIACHVLSATVKGQGYSLPA 258

Query: 269 DIQYVDARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQE 328
           D+QYVD RWE+PV LPLRDC+AQEL +LC +D LKT +      +GIN LV+SF+  L+E
Sbjct: 259 DVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHLDRPSNGINSLVASFIKRLRE 318

Query: 329 ENPSRESTIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAV 388
           ENPSRE TIVRTA KL PF+F++       + +P  ++ R K        S  SE  C +
Sbjct: 319 ENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLRPKFQKFDSDESTFSEILCLM 378

Query: 389 CNSPLNISDVQTLRSCE-TSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVA 448
           C SP + S++Q L S +  + +  ++ +   C SC FQILP      E F SLLP     
Sbjct: 379 CGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQILPAGENLNEHFFSLLPKLWTG 438

Query: 449 RAIHGSTGNSNSLREQIQEFLLSDDE 473
           +    S  +S  LR+QI+E+LL +++
Sbjct: 439 KMDTISDSHS-LLRDQIEEYLLEEND 461

[Close]

BLAST of Moc05g27520 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 7.9e-24
Identity = 127/515 (24.66%), Postives = 208/515 (40.39%), Query Frame = 0


Query: 53  LCFKCKSNERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPC 112
           +C KCK +        GD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 113 SRVALQFVHELQVKAHKNFEASRDRSLQV-FGVGVAYIDETSACSIPSHEMDSAIETIQS 172
           S   ++ V E           SRD   ++ F  G+ +IDE +AC +   E    +  I S
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQQILSEICS 143

Query: 173 VVSNLRPPSKELHIVQIESVYS------------------------------------SN 232
           V+   + P    HIV +E V+S                                    S 
Sbjct: 144 VLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 233 LGDRRE---------------------------KLKKLLDTVNDVTGKEDLLSHLRMLSL 292
           + +R E                           KLK+L  +   +T K+ LL  LR   +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 293 QKIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLRDC 352
             IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R+ 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 353 IAQELHMLCCVDGLKTV-----ELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGK 412
            ++E+        +  +     +   +  S I  L   FV  LQ + PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVSPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 413 LTPFNFSRIPDLDESNNV---------PLATKRRQ----KRNNVKH---------VNSIS 472
           L   N S+I D D+             PL T+  +        + H          N ++
Sbjct: 384 L---NVSKI-DADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLA 443

Query: 473 SESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLL 476
           + S  + C       +     +C++            C SC+  +  KD  S    L +L
Sbjct: 444 NNSDKSCCQGGQGCKEAGYGDTCQSRALQTPSFVHMLCYSCRLTV--KDMQS----LDVL 503

[Close]

BLAST of Moc05g27520 vs. ExPASy Swiss-Prot
Match: Q32NV1 (Cytoplasmic tRNA 2-thiolation protein 2-B OS=Xenopus laevis OX=8355 GN=ctu2-b PE=2 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 2.0e-22
Identity = 126/515 (24.47%), Postives = 207/515 (40.19%), Query Frame = 0


Query: 53  LCFKCKSNERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPC 112
           +C KCK +        GD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 113 SRVALQFVHELQVKAHKNFEASRDRSLQV-FGVGVAYIDETSACSIPSHEMDSAIETIQS 172
           S   ++ V E           SRD   ++ F  G+ +IDE +AC +   E    +  I S
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQQILSEICS 143

Query: 173 VVSNLRPPSKELHIVQIESVYS------------------------------------SN 232
           V+   + P    HIV +E V+S                                    S 
Sbjct: 144 VLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 233 LGDRRE---------------------------KLKKLLDTVNDVTGKEDLLSHLRMLSL 292
           + +R E                           KLK+L  +   +T K+ LL  LR   +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSRPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 293 QKIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLRDC 352
             IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R+ 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 353 IAQEL-----HMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGK 412
            ++E+             +  ++   +  S I  L   FV  LQ + PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVLPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 413 LTPFNFSRIPDLDESNNV---------PLATKRRQ----KRNNVKH---------VNSIS 472
           L   N S I D D+             PL T+  +        + H          N ++
Sbjct: 384 L---NVS-IIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLA 443

Query: 473 SESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLL 476
           + S  + C       +     +C++            C SC+  +  KD  S    L +L
Sbjct: 444 NNSDKSCCQGGQGCKEAGYGDTCQSRALQTPSFVHMLCYSCRLTV--KDMQS----LDVL 503

[Close]

BLAST of Moc05g27520 vs. ExPASy TrEMBL
Match: A0A6J1DEW9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111019863 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 9.4e-270
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60

Query: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120
           ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV
Sbjct: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120

Query: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180
           HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS
Sbjct: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180

Query: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRL 240
           KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRL
Sbjct: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRL 240

Query: 241 LVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDG 300
           LVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDG
Sbjct: 241 LVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDG 300

Query: 301 LKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNV 360
           LKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNV
Sbjct: 301 LKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNV 360

Query: 361 PLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSS 420
           PLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSS
Sbjct: 361 PLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSS 420

Query: 421 CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES
Sbjct: 421 CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 475

[Close]

BLAST of Moc05g27520 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.2e-220
Identity = 401/476 (84.24%), Postives = 425/476 (89.29%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQKIA ENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           L++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC+AQEL+MLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKT EL K SFSGIN LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDL+ESN 
Sbjct: 301 GLKTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN+SDVQTLRS E   + ++IL+GT CS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAG-QVSSILAGTCCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           SCQFQILPKDS SQEL LSLLPPSMVARA+HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 421 SCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of Moc05g27520 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 2.9e-218
Identity = 398/476 (83.61%), Postives = 423/476 (88.87%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NK +  AA       T GD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSL VFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQKIA ENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           L++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC+AQEL+MLCC D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKTVEL K SFSGIN LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDL+ESN 
Sbjct: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN+SDVQTLRS E   + ++IL+GT CS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAG-QVSSILAGTCCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           SCQFQILPKDS SQEL LSLLPPSMVARA+HGS  NSNSLRE+IQEFLLSDDE ES
Sbjct: 421 SCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

[Close]

BLAST of Moc05g27520 vs. ExPASy TrEMBL
Match: A0A0A0LC57 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G816060 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.2e-213
Identity = 388/476 (81.51%), Postives = 418/476 (87.82%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQ DE T+EQ SPP+++ +  AAME+  V T  D  NT+LCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMS+SGVGDDGRFCS CFRSNLFAKFR AVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYI+ET+ACSIPS E+D  ++TIQSVVS+L  P
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
            K LHIVQIESVYSSNL D REKLKKLLD VNDVTGKEDL+S+LRMLSLQKIA ENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           LLVGTCTSRIA HVISSTVKGQGYSLP D+QY+DARWEVPV LPL DC+AQEL+MLCCVD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKTVEL K S SGIN LVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDL+ESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VPLATKRRQ+RNN KHVNS+SSESFCAVCN PLN SDV T  +        + + G  CS
Sbjct: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHA-------GSSILGACCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           SCQFQILPKDS+SQELFLSLLPPSMVARA HGS GNSNSLR++IQEFLLSDDEAES
Sbjct: 421 SCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

[Close]

BLAST of Moc05g27520 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.7e-210
Identity = 382/475 (80.42%), Postives = 415/475 (87.37%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSS CQSNCYRNQ DE T+EQ SPP+++   AA  ES  +  + + NT+LCFKCKS 
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGES-RITNDDNSNTNLCFKCKSK 60

Query: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120
           ERMS+SGVGDDGR CS CFRSNLFAKFR AVTSNAMISPSDKVLVAFSGGPCSRVALQFV
Sbjct: 61  ERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120

Query: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180
           HE+QVKAHKNFEASRDRSLQVFGVGVAYI+ET+A SIPS E+D  I+TIQSVVS+L  P 
Sbjct: 121 HEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQPR 180

Query: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNRL 240
           K+LHIV IE+VYSSNL D REKLKKLLD V+DVTGKEDL+S+LRMLSLQKIA ENGYNRL
Sbjct: 181 KDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNRL 240

Query: 241 LVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDG 300
           LVGTCTSRIA HVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DC+AQEL+MLCC+DG
Sbjct: 241 LVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCIDG 300

Query: 301 LKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNNV 360
           LKTVEL K S SGIN LVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDL+ESNNV
Sbjct: 301 LKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNV 360

Query: 361 PLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSS 420
           PLATKRRQKRNN KHVNS+SSESFCAVCN PLN SDVQT  +       ++ + G  CSS
Sbjct: 361 PLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA-------SSSILGACCSS 420

Query: 421 CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 476
           CQFQILPKDS S ELFLSLLPPSMVARA HGS GNSNSLR +IQEFLLSDD+AES
Sbjct: 421 CQFQILPKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]

BLAST of Moc05g27520 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.2e-144
Identity = 274/473 (57.93%), Postives = 346/473 (73.15%), Query Frame = 0


Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSS C+S CY  + D           +K    A        + G  +  +C KCK N
Sbjct: 1   MACNSSGCESGCYDREKDN---------GSKIVDDAV-------SGGGNHESVCVKCKCN 60

Query: 61  ERMS-SSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
             M+   G  DDGRFC+ CFR+N+F KFRLAVTS+AMI+PSD VLVAFSGG  SRV+LQF
Sbjct: 61  APMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHELQ+KA KN+EASRDRSL VFGVGVA++DET+A    S EM  AIE ++  VS L PP
Sbjct: 121 VHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240
           +K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDLL HL+MLSLQK+A ENGYNR
Sbjct: 181 AKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNR 240

Query: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300
           L++G+CTSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLRDC+  E+  LC +D
Sbjct: 241 LVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLD 300

Query: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360
           GLKTVELA  S  GIN LVSSFVALLQEENPSRE TIVRTA KLTPF F++IP+ D+S N
Sbjct: 301 GLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS-N 360

Query: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420
           VP+AT+RR KR N+K+  S+++E+FC +CN PLN SD   L + E   E +++L    CS
Sbjct: 361 VPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQE-SDVLYAACCS 420

Query: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDE 473
           SC+FQILP+D +S E F S LP  M+++  H    +   LRE+I++ LL DDE
Sbjct: 421 SCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

The following BLAST results are available for this feature:

BLAST of Moc05g27520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152059.1	1.9e-269	100.00	cytoplasmic tRNA 2-thiolation protein 2 [Momordica charantia]	[more]
XP_038905927.1	1.1e-221	83.97	cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida]	[more]
XP_023539180.1	2.2e-220	84.45	cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022939604.1	1.1e-219	84.24	cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata]	[more]
XP_022971223.1	5.9e-218	83.61	cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima]	[more]

BLAST of Moc05g27520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65628	1.7e-143	57.93	Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 ...	[more]
A2ZM73	1.7e-103	46.64	Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 G...	[more]
Q2QMW0	1.9e-102	46.19	Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q08B12	7.9e-24	24.66	Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE...	[more]
Q32NV1	2.0e-22	24.47	Cytoplasmic tRNA 2-thiolation protein 2-B OS=Xenopus laevis OX=8355 GN=ctu2-b PE...	[more]

BLAST of Moc05g27520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DEW9	9.4e-270	100.00	Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1FHP7	5.2e-220	84.24	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1I684	2.9e-218	83.61	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]
A0A0A0LC57	1.2e-213	81.51	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G8160...	[more]
A0A1S3B7H9	3.7e-210	80.42	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 ...	[more]

BLAST of Moc05g27520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35910.1	1.2e-144	57.93	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019407	Cytoplasmic tRNA 2-thiolation protein 2	PFAM	PF10288	CTU2	coord: 314..406 e-value: 1.3E-6 score: 28.5
IPR019407	Cytoplasmic tRNA 2-thiolation protein 2	PANTHER	PTHR20882	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2	coord: 48..469
IPR019407	Cytoplasmic tRNA 2-thiolation protein 2	HAMAP	MF_03054	CTU2	coord: 50..429 score: 20.646618
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 53..413 e-value: 2.8E-40 score: 140.3
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 78..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g27520.1	Moc05g27520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010311	lateral root formation
biological_process	GO:0032447	protein urmylation
biological_process	GO:0002143	tRNA wobble position uridine thiolation
biological_process	GO:0034227	tRNA thio-modification
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0000049	tRNA binding