Moc05g27390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g27390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Transposon TX1 uncharacterized 149 kDa protein
Location	chr5: 19749804 .. 19750598 (-)
RNA-Seq Expression	Moc05g27390
Synteny	Moc05g27390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATATCCTTGTCCCTACTACCTCTGAGCCCCTCGAAATTCAGCCCCTCCACTTGTTTGATCCCACCATACACCCTTCGGAGTATCTACAGGTGGTCATCCCTTGGCTCTGTACTATGGGTATGGGCATTTACCCTATTCCTCAAAAAGTCACAAAGAAAATGCTCTCCAACAAAAAAGAGAAGGATAAAAAAGAGAACTTGTTGGCCTCGTATCAACCATCAACTATGACTTTCACCAAACCAAGAATTTATCCGGCCGGTCTACCACTTCTTGATGATTATTGTCTCTTGGAATGTAAGAGGTCTTGGCTCATGGGACAAATGGGCCTTGATTAAAAGCCTTATCAACAAGCACAACCCTACCATAGTCATTCTCCAAGAGACAAAGCTGGAAAAGATTGACCGCCTCACTATTAAGTCTCTTTGGAGCTCTCGAGATATCAGATGGATAGCTATTGATGCATCATGCTCATCAGGGGTATTCTTATCATGTGGATGACCCATCTTTACTTGTTTCAAAACATCACCATGAGTCTTCACACAATAACCATCTTTATTAATCTTTCTGATGGCTTCAATTTTTGGCTTACAGGGGTCTACGGGCCAACAAGAACAGAAGAGAAAGCTGGTTTCTGGAATGAACTGATGGACCTCTCCTTTCTTTGCTCGGATTTTTGGCTATTGGGGGATGATTTTAACACCATTCGATGGACACAAGAAAAATCTTCTTATCGCCGACCGAATAGGAATATGAGTTGCTTCAATTCCTTCATTGAGAGAGCAGAGCTATAG

mRNA sequence

ATGGATATCCTTGTCCCTACTACCTCTGAGCCCCTCGAAATTCAGCCCCTCCACTTGTTTGATCCCACCATACACCCTTCGGAGTATCTACAGGTGGTCATCCCTTGGCTCTGTACTATGGGTCTTGGCTCATGGGACAAATGGGCCTTGATTAAAAGCCTTATCAACAAGCACAACCCTACCATAGTCATTCTCCAAGAGACAAAGCTGGAAAAGATTGACCGCCTCACTATTAAGTCTCTTTGGAGCTCTCGAGATATCAGATGGATAGCTATTGATGCATCATGCTCATCAGGGGTATTCTTATCATGGGTCTACGGGCCAACAAGAACAGAAGAGAAAGCTGGTTTCTGGAATGAACTGATGGACCTCTCCTTTCTTTGCTCGGATTTTTGGCTATTGGGGGATGATTTTAACACCATTCGATGGACACAAGAAAAATCTTCTTATCGCCGACCGAATAGGAATATGAGTTGCTTCAATTCCTTCATTGAGAGAGCAGAGCTATAG

Coding sequence (CDS)

ATGGATATCCTTGTCCCTACTACCTCTGAGCCCCTCGAAATTCAGCCCCTCCACTTGTTTGATCCCACCATACACCCTTCGGAGTATCTACAGGTGGTCATCCCTTGGCTCTGTACTATGGGTCTTGGCTCATGGGACAAATGGGCCTTGATTAAAAGCCTTATCAACAAGCACAACCCTACCATAGTCATTCTCCAAGAGACAAAGCTGGAAAAGATTGACCGCCTCACTATTAAGTCTCTTTGGAGCTCTCGAGATATCAGATGGATAGCTATTGATGCATCATGCTCATCAGGGGTATTCTTATCATGGGTCTACGGGCCAACAAGAACAGAAGAGAAAGCTGGTTTCTGGAATGAACTGATGGACCTCTCCTTTCTTTGCTCGGATTTTTGGCTATTGGGGGATGATTTTAACACCATTCGATGGACACAAGAAAAATCTTCTTATCGCCGACCGAATAGGAATATGAGTTGCTTCAATTCCTTCATTGAGAGAGCAGAGCTATAG

Protein sequence

MDILVPTTSEPLEIQPLHLFDPTIHPSEYLQVVIPWLCTMGLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDASCSSGVFLSWVYGPTRTEEKAGFWNELMDLSFLCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCFNSFIERAEL

Homology

[Close]

BLAST of Moc05g27390 vs. NCBI nr
Match: XP_022158956.1 (uncharacterized protein LOC111025405 [Momordica charantia])

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-25
Identity = 72/163 (44.17%), Postives = 88/163 (53.99%), Query Frame = 0


Query: 41  GLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDAS----- 100
           GL SW K ALIK  I++ NP +VILQETKL  +D L +KSLWS+  I W A+DAS     
Sbjct: 10  GLDSWKKGALIKQFISRLNPNVVILQETKLSYMDILIVKSLWSAHGINWSALDASGMASG 69

Query: 101 --------------------------CSSGVFLSWV---YGPTRTEEKAGFWNELMDLSF 160
                                     C S  FL WV   YGP+ TE    FW EL+DLS 
Sbjct: 70  ILILWNDPDLKAAEMIEGVFSLTINFCLSDGFLFWVSGIYGPSTTEFHYLFWQELLDLSD 129

Query: 161 LCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCFNSFIERAEL 170
           LC + W+L  DFN  RW+ EKS+ R   ++M  FNSFIE + L
Sbjct: 130 LCENHWILAGDFNVTRWSWEKSNGRPLTKSMWLFNSFIEDSSL 172

[Close]

BLAST of Moc05g27390 vs. NCBI nr
Match: XP_022145142.1 (uncharacterized protein LOC111014657 [Momordica charantia])

HSP 1 Score: 108.6 bits (270), Expect = 5.3e-20
Identity = 65/163 (39.88%), Postives = 78/163 (47.85%), Query Frame = 0


Query: 41  GLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDAS-CSSG 100
           GLGS  K A IK  I    P IVIL ETK   I+   IKSLWSS  I W ++DAS  S G
Sbjct: 10  GLGSASKRATIKDTITSLCPDIVILSETKSSSINNKFIKSLWSSISIAWASLDASGASGG 69

Query: 101 VFLSW---------------------------------VYGPTRTEEKAGFWNELMDLSF 160
           + L W                                 VY P + +++  FW EL DL+ 
Sbjct: 70  IILLWDQLSTSAVEVICGHFSISVHFKLADNFTWWLTGVYSPVKQKKRKLFWQELFDLNG 129

Query: 161 LCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCFNSFIERAEL 170
           LC   WLLG+DFN  RW+ E SS   P   M+ FN FI+ A L
Sbjct: 130 LCGPIWLLGEDFNIYRWSHETSSANPPQTGMNKFNHFIDFAGL 172

[Close]

BLAST of Moc05g27390 vs. NCBI nr
Match: XP_022154822.1 (uncharacterized protein LOC111021983 [Momordica charantia])

HSP 1 Score: 98.2 bits (243), Expect = 7.2e-17
Identity = 50/129 (38.76%), Postives = 69/129 (53.49%), Query Frame = 0


Query: 41  GLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDASCSSGV 100
           G+  + K   +K  I K NP +V+LQETKL++IDR+ IKSLWSS+D+ W  +++      
Sbjct: 10  GINGYKKRLKVKKTIMKSNPDVVLLQETKLQQIDRIIIKSLWSSKDVGWACLNSK----- 69

Query: 101 FLSWVYGPTRTEEKAGFWNELMDLSFLCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCF 160
                        +   W+EL D+S     FW LG DFN  RW  +KSS  R  R+M  F
Sbjct: 70  ---------DYRNRKRLWSELRDISGFSEKFWCLGGDFNVSRWPSDKSSGGRITRSMRKF 124

Query: 161 NSFIERAEL 170
           N  I   +L
Sbjct: 130 NCIIGELDL 124

[Close]

BLAST of Moc05g27390 vs. NCBI nr
Match: RVW67200.1 (hypothetical protein CK203_065147 [Vitis vinifera])

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-16
Identity = 51/138 (36.96%), Postives = 72/138 (52.17%), Query Frame = 0


Query: 39  TMGLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDA-SCS 98
           T GLGS  K  +++  ++  NP +V+LQETK E  DR  + S+W  + + W+A+ A    
Sbjct: 30  TRGLGSRKKRRIVRRFLSTQNPDVVLLQETKREIWDRRFVSSVWKGKCLDWVALPACGAF 89

Query: 99  SGVFLSW------VYGPTRTEEKAGFWNELMDLSFLCSDFWLLGDDFNTIRWTQEKSSYR 158
            G+ + W      +YGP +   +  FW EL DL  L    W +G DFN IR   EK    
Sbjct: 90  GGIVILWDSVKFNLYGPNKAVWRKDFWMELQDLHGLTFPRWCVGGDFNVIRRISEKMGDS 149

Query: 159 RPNRNMSCFNSFIERAEL 170
           R   NM CF+ FI  + L
Sbjct: 150 RLTVNMRCFDEFIRESGL 167

[Close]

BLAST of Moc05g27390 vs. NCBI nr
Match: VVA20479.1 (Hypothetical predicted protein, partial [Prunus dulcis])

HSP 1 Score: 95.5 bits (236), Expect = 4.6e-16
Identity = 59/174 (33.91%), Postives = 78/174 (44.83%), Query Frame = 0


Query: 30  LQVVIPWLCTMGLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRW 89
           L  +I W    GLGS  K  L+K  + +  P IVIL ETK E +DR  +  +W SR   W
Sbjct: 7   LMKIISW-NIRGLGSRRKRLLVKEQLRRLKPDIVILLETKKEIVDRQLVTGVWGSRFKEW 66

Query: 90  ----------------------------------IAIDASCSSGVFLSWVYGPTRTEEKA 149
                                             I I  +  +  +LS +YGP R  E+ 
Sbjct: 67  VFSPSLGRSGGIAVLWNSQSVSVIDSMVGDFSVSIRIVENIGTDWWLSGIYGPCRQRERI 126

Query: 150 GFWNELMDLSFLCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCFNSFIERAEL 170
            FW EL DL   C D W LG DFN +R++ EKS+  R  ++M  FN FI+   L
Sbjct: 127 SFWEELADLYGYCGDKWCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNL 179

[Close]

BLAST of Moc05g27390 vs. ExPASy TrEMBL
Match: A0A6J1E2G6 (uncharacterized protein LOC111025405 OS=Momordica charantia OX=3673 GN=LOC111025405 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 7.0e-26
Identity = 72/163 (44.17%), Postives = 88/163 (53.99%), Query Frame = 0


Query: 41  GLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDAS----- 100
           GL SW K ALIK  I++ NP +VILQETKL  +D L +KSLWS+  I W A+DAS     
Sbjct: 10  GLDSWKKGALIKQFISRLNPNVVILQETKLSYMDILIVKSLWSAHGINWSALDASGMASG 69

Query: 101 --------------------------CSSGVFLSWV---YGPTRTEEKAGFWNELMDLSF 160
                                     C S  FL WV   YGP+ TE    FW EL+DLS 
Sbjct: 70  ILILWNDPDLKAAEMIEGVFSLTINFCLSDGFLFWVSGIYGPSTTEFHYLFWQELLDLSD 129

Query: 161 LCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCFNSFIERAEL 170
           LC + W+L  DFN  RW+ EKS+ R   ++M  FNSFIE + L
Sbjct: 130 LCENHWILAGDFNVTRWSWEKSNGRPLTKSMWLFNSFIEDSSL 172

[Close]

BLAST of Moc05g27390 vs. ExPASy TrEMBL
Match: A0A6J1CVN2 (uncharacterized protein LOC111014657 OS=Momordica charantia OX=3673 GN=LOC111014657 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.6e-20
Identity = 65/163 (39.88%), Postives = 78/163 (47.85%), Query Frame = 0


Query: 41  GLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDAS-CSSG 100
           GLGS  K A IK  I    P IVIL ETK   I+   IKSLWSS  I W ++DAS  S G
Sbjct: 10  GLGSASKRATIKDTITSLCPDIVILSETKSSSINNKFIKSLWSSISIAWASLDASGASGG 69

Query: 101 VFLSW---------------------------------VYGPTRTEEKAGFWNELMDLSF 160
           + L W                                 VY P + +++  FW EL DL+ 
Sbjct: 70  IILLWDQLSTSAVEVICGHFSISVHFKLADNFTWWLTGVYSPVKQKKRKLFWQELFDLNG 129

Query: 161 LCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCFNSFIERAEL 170
           LC   WLLG+DFN  RW+ E SS   P   M+ FN FI+ A L
Sbjct: 130 LCGPIWLLGEDFNIYRWSHETSSANPPQTGMNKFNHFIDFAGL 172

[Close]

BLAST of Moc05g27390 vs. ExPASy TrEMBL
Match: A0A6J1DMQ9 (uncharacterized protein LOC111021983 OS=Momordica charantia OX=3673 GN=LOC111021983 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 3.5e-17
Identity = 50/129 (38.76%), Postives = 69/129 (53.49%), Query Frame = 0


Query: 41  GLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDASCSSGV 100
           G+  + K   +K  I K NP +V+LQETKL++IDR+ IKSLWSS+D+ W  +++      
Sbjct: 10  GINGYKKRLKVKKTIMKSNPDVVLLQETKLQQIDRIIIKSLWSSKDVGWACLNSK----- 69

Query: 101 FLSWVYGPTRTEEKAGFWNELMDLSFLCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCF 160
                        +   W+EL D+S     FW LG DFN  RW  +KSS  R  R+M  F
Sbjct: 70  ---------DYRNRKRLWSELRDISGFSEKFWCLGGDFNVSRWPSDKSSGGRITRSMRKF 124

Query: 161 NSFIERAEL 170
           N  I   +L
Sbjct: 130 NCIIGELDL 124

[Close]

BLAST of Moc05g27390 vs. ExPASy TrEMBL
Match: M5WJ76 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa015871mg PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 3.5e-17
Identity = 59/174 (33.91%), Postives = 79/174 (45.40%), Query Frame = 0


Query: 30  LQVVIPWLCTMGLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRW 89
           L  +I W    GLGS  K  L+K  + +  P IVIL ETK E +DR  +  +W SR   W
Sbjct: 322 LMKIISW-NIRGLGSRRKRLLVKEQLRRLKPDIVILLETKKEIVDRQLVAGVWGSRFKEW 381

Query: 90  ----------------------------------IAIDASCSSGVFLSWVYGPTRTEEKA 149
                                             I I+ +  +  +LS +YGP R  E+ 
Sbjct: 382 VFSPSLGRSGGIAVLWNSQSVSVIDSMVGEFSVSIRIEENIGTDWWLSGIYGPCRQRERN 441

Query: 150 GFWNELMDLSFLCSDFWLLGDDFNTIRWTQEKSSYRRPNRNMSCFNSFIERAEL 170
            FW EL DL   C D W LG DFN +R++ EKS+  R  ++M  FN FI+   L
Sbjct: 442 SFWEELADLYGYCGDMWCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNL 494

[Close]

BLAST of Moc05g27390 vs. ExPASy TrEMBL
Match: A0A438G4S3 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_065147 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 7.7e-17
Identity = 51/138 (36.96%), Postives = 72/138 (52.17%), Query Frame = 0


Query: 39  TMGLGSWDKWALIKSLINKHNPTIVILQETKLEKIDRLTIKSLWSSRDIRWIAIDA-SCS 98
           T GLGS  K  +++  ++  NP +V+LQETK E  DR  + S+W  + + W+A+ A    
Sbjct: 30  TRGLGSRKKRRIVRRFLSTQNPDVVLLQETKREIWDRRFVSSVWKGKCLDWVALPACGAF 89

Query: 99  SGVFLSW------VYGPTRTEEKAGFWNELMDLSFLCSDFWLLGDDFNTIRWTQEKSSYR 158
            G+ + W      +YGP +   +  FW EL DL  L    W +G DFN IR   EK    
Sbjct: 90  GGIVILWDSVKFNLYGPNKAVWRKDFWMELQDLHGLTFPRWCVGGDFNVIRRISEKMGDS 149

Query: 159 RPNRNMSCFNSFIERAEL 170
           R   NM CF+ FI  + L
Sbjct: 150 RLTVNMRCFDEFIRESGL 167

The following BLAST results are available for this feature:

BLAST of Moc05g27390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158956.1	1.4e-25	44.17	uncharacterized protein LOC111025405 [Momordica charantia]	[more]
XP_022145142.1	5.3e-20	39.88	uncharacterized protein LOC111014657 [Momordica charantia]	[more]
XP_022154822.1	7.2e-17	38.76	uncharacterized protein LOC111021983 [Momordica charantia]	[more]
RVW67200.1	1.6e-16	36.96	hypothetical protein CK203_065147 [Vitis vinifera]	[more]
VVA20479.1	4.6e-16	33.91	Hypothetical predicted protein, partial [Prunus dulcis]	[more]

BLAST of Moc05g27390 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc05g27390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E2G6	7.0e-26	44.17	uncharacterized protein LOC111025405 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1CVN2	2.6e-20	39.88	uncharacterized protein LOC111014657 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1DMQ9	3.5e-17	38.76	uncharacterized protein LOC111021983 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
M5WJ76	3.5e-17	33.91	Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=...	[more]
A0A438G4S3	7.7e-17	36.96	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_065147 PE=4 SV=1	[more]

BLAST of Moc05g27390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	GENE3D	3.60.10.10	Endonuclease/exonuclease/phosphatase	coord: 36..169 e-value: 2.7E-10 score: 42.4
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	SUPERFAMILY	56219	DNase I-like	coord: 41..167
IPR004808	AP endonuclease 1	PANTHER	PTHR22748	AP ENDONUCLEASE	coord: 41..154
None	No IPR available	PANTHER	PTHR22748:SF11	DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE CHLOROPLASTIC	coord: 41..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g27390.1	Moc05g27390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
molecular_function	GO:0005488	binding
molecular_function	GO:0004518	nuclease activity