Moc05g16000 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g16000
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr5: 12056492 .. 12057912 (+)
RNA-Seq Expression	Moc05g16000
Synteny	Moc05g16000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGGAGCAGATCGATACTCTTGGAAACAACAACCGTATGGTCGGACACCTCGATACGCTCCATATTATTACCCAATGCCATACAGGTATAATTAGCTAAAAGTGAACAATACAACTTCACATTACTCTTCTTATGCCTCTCAAAATTTTCGACCACCGACCAGTCAAAATTTTCATCCTAGGGGTCAGCAGAATAATACATTCTATTCCCAAGGGCAGCAGAATAATAGGGGGCACGTAAACAGACTCAGTTCGACCCAATCCCTATGACTTATGTATTAGTGGAGTACCTATGCCCAAACCTCAAGTATAAAAGGGTATGATGAAAGTCTGACGTGCCCGTTCCATGCTAGGGCAAAAAGACATTCTCTAAAGCAATGCAATCATTTTCAAAAAAGAGTCCAAGAGTTGTTAGACATAAAAGCCTTTACAGTTACAAAATCTCACCAGAAGAAAGGGATAAATGTCGTGGAGGATGTCTCAACTGCTGAAAGCTCAAATGCTTCGCTTAAGCCTAAACCTCTCACCATCTTTTACCGTGAAAAGCCTGATGCACCCAACAGCAGTAGAACACTAATCACTATCACGGTTCCAGCTCCTTTCGAGTATAAAAGTTCCAAAGCAGTACCTTGGAGATATGAGTACAAGGTAACTGTTGGGCAAGAGGTACAATCCCTTAACTTCCAGTTGATAATATCACTAGAGTAGGAGGCTTGACACGAACTGAGAGATGTTATGCATTGGATAGCTTGCTAAAACACATGAATGAGTCTACCAGTGAAAAGAAGAAGGAGAAAGCAAGTGAGAATAAAAAAAATAATGCAAAGGAAGATAAGAAGGGGAAGGCCAAACTTAACGAGGATGTTTATGATGAATTAGAGGAAGCAATTGTTGTAAAGGATGCAAATCCTAAACAACCCGTGTCTGAGGAAGATACTCAGAAGTTTCTAAAGTTAGTAAAGCAAAGTGAATACAAAGTTATCGAACAACTAGGTCGAACACCCGCAAAGATCTCTTTATTATCTTTGTTGTTATCCTCTGAAGCGCATCAGAATAAATTGTTGGAGGTCTTGAAGCAGGCTTTCGTTTCACAAGATATCACAGTGGATAATTTGAACAACGTTGTTGGGAATATAGCTGCATCTAGCTCCATCACTTTTACAGATGAGAAGATACCACCAGAGGGTACAGGACACACCAAAACCCTCCATATAACAGTTAAGTATAAAAACTTCCTAGTAGCAAAAGTCCTCGTTGATAATGGATCTTCCTTAAACATAATGCCAAGATCCACACTAGAGAAGTTACCAGTCGATATGTCCCATATGAGGCCTAGTACTATGATAGTGACAGCTTTCGATGGAGCTTGCAGCACTGTTGTTGGGGATATTAAGATCCCGATCCAGATAGGTCATTGA

mRNA sequence

ATGATGGGAGCAGATCGATACTCTTGGAAACAACAACCGTATGGTCGGACACCTCGATACGCTCCATATTATTACCCAATGCCATACAGGGCAAAAAGACATTCTCTAAAGCAATGCAATCATTTTCAAAAAAGAGTCCAAGAGTTGTTAGACATAAAAGCCTTTACAGTTACAAAATCTCACCAGAAGAAAGGGATAAATGTCGTGGAGGATGTCTCAACTGCTGAAAGCTCAAATGCTTCGCTTAAGCCTAAACCTCTCACCATCTTTTACCGTGAAAAGCCTGATGCACCCAACAGCAGTAGAACACTAATCACTATCACGGTTCCAGCTCCTTTCGAGTATAAAAGTTCCAAAGCAGTACCTTGGAGATATGAGTACAAGGTAACTGTTGGGCAAGAGTCTACCAGTGAAAAGAAGAAGGAGAAAGCAAGTGAGAATAAAAAAAATAATGCAAAGGAAGATAAGAAGGGGAAGGCCAAACTTAACGAGGATGTTTATGATGAATTAGAGGAAGCAATTGTTGTAAAGGATGCAAATCCTAAACAACCCGTGTCTGAGGAAGATACTCAGAAGTTTCTAAAGTTAGTAAAGCAAAGTGAATACAAAGTTATCGAACAACTAGGTCGAACACCCGCAAAGATCTCTTTATTATCTTTGTTGTTATCCTCTGAAGCGCATCAGAATAAATTGTTGGAGGTCTTGAAGCAGGCTTTCGTTTCACAAGATATCACAGTGGATAATTTGAACAACGTTGTTGGGAATATAGCTGCATCTAGCTCCATCACTTTTACAGATGAGAAGATACCACCAGAGGGTACAGGACACACCAAAACCCTCCATATAACAGTTAAGTATAAAAACTTCCTAGTAGCAAAAGTCCTCGTTGATAATGGATCTTCCTTAAACATAATGCCAAGATCCACACTAGAGAAGTTACCAGTCGATATGTCCCATATGAGGCCTAGTACTATGATAGTGACAGCTTTCGATGGAGCTTGCAGCACTGTTGTTGGGGATATTAAGATCCCGATCCAGATAGGTCATTGA

Coding sequence (CDS)

ATGATGGGAGCAGATCGATACTCTTGGAAACAACAACCGTATGGTCGGACACCTCGATACGCTCCATATTATTACCCAATGCCATACAGGGCAAAAAGACATTCTCTAAAGCAATGCAATCATTTTCAAAAAAGAGTCCAAGAGTTGTTAGACATAAAAGCCTTTACAGTTACAAAATCTCACCAGAAGAAAGGGATAAATGTCGTGGAGGATGTCTCAACTGCTGAAAGCTCAAATGCTTCGCTTAAGCCTAAACCTCTCACCATCTTTTACCGTGAAAAGCCTGATGCACCCAACAGCAGTAGAACACTAATCACTATCACGGTTCCAGCTCCTTTCGAGTATAAAAGTTCCAAAGCAGTACCTTGGAGATATGAGTACAAGGTAACTGTTGGGCAAGAGTCTACCAGTGAAAAGAAGAAGGAGAAAGCAAGTGAGAATAAAAAAAATAATGCAAAGGAAGATAAGAAGGGGAAGGCCAAACTTAACGAGGATGTTTATGATGAATTAGAGGAAGCAATTGTTGTAAAGGATGCAAATCCTAAACAACCCGTGTCTGAGGAAGATACTCAGAAGTTTCTAAAGTTAGTAAAGCAAAGTGAATACAAAGTTATCGAACAACTAGGTCGAACACCCGCAAAGATCTCTTTATTATCTTTGTTGTTATCCTCTGAAGCGCATCAGAATAAATTGTTGGAGGTCTTGAAGCAGGCTTTCGTTTCACAAGATATCACAGTGGATAATTTGAACAACGTTGTTGGGAATATAGCTGCATCTAGCTCCATCACTTTTACAGATGAGAAGATACCACCAGAGGGTACAGGACACACCAAAACCCTCCATATAACAGTTAAGTATAAAAACTTCCTAGTAGCAAAAGTCCTCGTTGATAATGGATCTTCCTTAAACATAATGCCAAGATCCACACTAGAGAAGTTACCAGTCGATATGTCCCATATGAGGCCTAGTACTATGATAGTGACAGCTTTCGATGGAGCTTGCAGCACTGTTGTTGGGGATATTAAGATCCCGATCCAGATAGGTCATTGA

Protein sequence

MMGADRYSWKQQPYGRTPRYAPYYYPMPYRAKRHSLKQCNHFQKRVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPKPLTIFYREKPDAPNSSRTLITITVPAPFEYKSSKAVPWRYEYKVTVGQESTSEKKKEKASENKKNNAKEDKKGKAKLNEDVYDELEEAIVVKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSEAHQNKLLEVLKQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVKYKNFLVAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPIQIGH

Homology

[Close]

BLAST of Moc05g16000 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 465.3 bits (1196), Expect = 4.6e-127
Identity = 258/358 (72.07%), Postives = 286/358 (79.89%), Query Frame = 0


Query: 26  PMPYRAKRHSLKQCNHFQKRVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPK 85
           P    AK HSL+QCN F+ +VQELLD K  TV  SHQKKGIN+VEDVS AE S+ +LKPK
Sbjct: 498 PFHAGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINIVEDVSVAEGSSDALKPK 557

Query: 86  PLTIFYREKPDAPNSSRTLITITVPAPFEYKSSKAVPWRYEYKVTVGQ------------ 145
            LTIFY EKP+APN SR  ITITVPAPFEYKSSKAVPW+Y+ KVTVGQ            
Sbjct: 558 CLTIFYSEKPNAPNCSRKPITITVPAPFEYKSSKAVPWKYQCKVTVGQDVSSPPLPIDNI 617

Query: 146 -----------------------ESTSEKKKEKASENKKNNAKEDKKGKAKLNEDVYDEL 205
                                  E+TSEK KEKASE KK   +EDKKGKAKL+EDV+DEL
Sbjct: 618 TGVGGLTRTGRCYTPDSLLKCVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDEL 677

Query: 206 EEAIVVKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSEAHQNK 265
            EAIVVKD +PKQP+SEE+TQ+ LKLVKQSEYKVIEQLGRTPAKIS+LSLLLSSEAH+N 
Sbjct: 678 VEAIVVKDVSPKQPMSEEETQEILKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAHRNA 737

Query: 266 LLEVLKQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVKYKNFL 325
           LLE LKQAFVSQDITVDNL+NVVGNI+ +SSITFTDE+IPPEGTGHTK LHI++K KNFL
Sbjct: 738 LLEALKQAFVSQDITVDNLSNVVGNISXASSITFTDEEIPPEGTGHTKALHISIKCKNFL 797

Query: 326 VAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPIQIG 349
           +AKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S VVGDI+IPIQIG
Sbjct: 798 IAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQIG 855

[Close]

BLAST of Moc05g16000 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 59.7 bits (143), Expect = 5.8e-05
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 1   MMGADRYSWKQQPYGRTPRYAPYYYPMPY 30
           M+GADR+SWKQQPY RTPRY PYYYP PY
Sbjct: 254 MVGADRHSWKQQPYSRTPRYTPYYYPTPY 282

HSP 2 Score: 457.2 bits (1175), Expect = 1.3e-124
Identity = 255/353 (72.24%), Postives = 277/353 (78.47%), Query Frame = 0


Query: 31  AKRHSLKQCNHFQKRVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPKPLTIF 90
           AK HSL+QCN F+ +VQELLD K  T   SH KK  NVVED+  AE S+ SLKPKPLTIF
Sbjct: 541 AKGHSLEQCNXFRMKVQELLDSKILTXANSHXKKXTNVVEDILVAEGSSDSLKPKPLTIF 600

Query: 91  YREKPDAPNSSRTLITITVPAPFEYKSSKAVPWRYEYKVTVGQ----------------- 150
           YREKPDAP+ SR    ITVP PFEYKSSKAVPW+YE KVTVGQ                 
Sbjct: 601 YREKPDAPSCSRKPXXITVPXPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGG 660

Query: 151 ------------------ESTSEKKKEKASENKKNNAKEDKKGKAKLNEDVYDELEEAIV 210
                             E+TSEK KEKASE KK   +EDKKGKAKL+ED  DEL EAIV
Sbjct: 661 LTRTGRCYTPDSLLKRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDARDELVEAIV 720

Query: 211 VKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSEAHQNKLLEVL 270
           VKD +PKQP+SEE+TQ+FLKLVKQSEYKVIEQLGRTPA IS+LSLLLSSEAHQN LLE L
Sbjct: 721 VKDVSPKQPMSEEETQEFLKLVKQSEYKVIEQLGRTPANISILSLLLSSEAHQNALLEAL 780

Query: 271 KQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVKYKNFLVAKVL 330
           KQAFVSQDITVDNL+NVVGNI ASSSI+FTDE+IPPEGTGHTK LHI+VK KNFL+AKVL
Sbjct: 781 KQAFVSQDITVDNLSNVVGNITASSSISFTDEEIPPEGTGHTKALHISVKCKNFLIAKVL 840

Query: 331 VDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPIQIG 349
           VDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S VVGDI+IPIQIG
Sbjct: 841 VDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQIG 893

[Close]

BLAST of Moc05g16000 vs. NCBI nr
Match: XP_022143495.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia])

HSP 1 Score: 456.8 bits (1174), Expect = 1.6e-124
Identity = 262/363 (72.18%), Postives = 282/363 (77.69%), Query Frame = 0


Query: 26  PMPYRAKRHSLKQCNHFQKRVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNA----- 85
           P    AK H+L+QCN F+  VQELLD K  TV  SHQKKGINVVEDVS AE S A     
Sbjct: 433 PFHAGAKGHALEQCNSFRMIVQELLDSKILTVANSHQKKGINVVEDVSVAEGSIAEGSSD 492

Query: 86  SLKPKPLTIFYREKPDAPNSSRTLITITVPAPFEYKSSKAVPWRYEYKVTVGQ------- 145
           +LKPK LTIFY EKPDAPN SR  ITITVPAPFEYKSSKAVPW+YE KVTVGQ       
Sbjct: 493 ALKPKRLTIFYSEKPDAPNCSRKPITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPPL 552

Query: 146 ----------------------------ESTSEKKKEKASENKKNNAKEDKKGKAKLNED 205
                                       E+TSEK KEKASE KK   +EDKKGKAKL+ED
Sbjct: 553 PVDNITGVGGLTXTGRCYTPDSLLKRVSETTSEKNKEKASEKKKEKVEEDKKGKAKLHED 612

Query: 206 VYDELEEAIVVKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSE 265
           V+DEL EAIVVKD +PKQ V EE+ Q+FLKLVKQSEYKV EQLGRTPAKIS+LSLLLSSE
Sbjct: 613 VHDELVEAIVVKDVSPKQHVFEEEIQEFLKLVKQSEYKVTEQLGRTPAKISILSLLLSSE 672

Query: 266 AHQNKLLEVLKQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVK 325
           AH+N LLE LKQAFVSQDITVDNL+NVVGNI ASSSITFTDE+IPPEGTGHTK LHI+VK
Sbjct: 673 AHRNTLLEXLKQAFVSQDITVDNLSNVVGNITASSSITFTDEEIPPEGTGHTKALHISVK 732

Query: 326 YKNFLVAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPI 349
            KNFL+AKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S VVGDI+IPI
Sbjct: 733 CKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPI 792

[Close]

BLAST of Moc05g16000 vs. NCBI nr
Match: XP_022150030.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111018303 [Momordica charantia])

HSP 1 Score: 409.8 bits (1052), Expect = 2.3e-110
Identity = 235/339 (69.32%), Postives = 257/339 (75.81%), Query Frame = 0


Query: 45  RVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPKPLTIFYREKPDAPNSSRTL 104
           +VQELLD K  TV  SHQKK  NVVED+  AE S+ S+KPK LTIFYREKPDAP+ SR  
Sbjct: 2   KVQELLDSKILTVANSHQKKRTNVVEDILVAEGSSDSIKPKRLTIFYREKPDAPSCSRKP 61

Query: 105 ITITVPAPFEYKSSKAVPWRYEYKVTVGQ------------------------------- 164
           ITITVPAPFEYKSSKAVPW+YE KVTVGQ                               
Sbjct: 62  ITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLL 121

Query: 165 ----ESTSEKKKEKASENKKNNAKEDKKGKAKLNEDVYDELEEAIVVKDANPKQPVSEED 224
               E+TSEK KEKASE KK   +EDKKGKAKL+EDV+DEL                EE+
Sbjct: 122 KRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDEL---------------VEEE 181

Query: 225 TQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSEAHQNKLLEVLKQAFVSQDITVDNL 284
           TQ+FLKLVKQ+EYKVIEQLGRTPAKIS+LSLLLSSEAH+N LLE LKQAFVSQDITVDNL
Sbjct: 182 TQEFLKLVKQNEYKVIEQLGRTPAKISILSLLLSSEAHRNALLEALKQAFVSQDITVDNL 241

Query: 285 NNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVKYKNFLVAKVLVDNGSSLNIMPRST 344
           +NVVGNI ASS ITFTDE+IPPEGTGHTK LHI+VK KNFL+AKVLV NGSSLNIMPRST
Sbjct: 242 SNVVGNIMASSCITFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVGNGSSLNIMPRST 301

Query: 345 LEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPIQIG 349
           LEKLPVDMSHMRPST+IV AFDGA + VVGDI+IPIQIG
Sbjct: 302 LEKLPVDMSHMRPSTVIVRAFDGARNAVVGDIEIPIQIG 325

[Close]

BLAST of Moc05g16000 vs. NCBI nr
Match: XP_022155744.1 (uncharacterized protein LOC111022791 [Momordica charantia])

HSP 1 Score: 396.4 bits (1017), Expect = 2.6e-106
Identity = 222/308 (72.08%), Postives = 253/308 (82.14%), Query Frame = 0


Query: 45  RVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPKPLTIFYREKPDAPNSSRTL 104
           +VQELLD K  TV  SHQKK  NVVED+  AE S+ S+KPKPLTIFYREKPDAP+  + +
Sbjct: 2   KVQELLDSKILTVANSHQKKRTNVVEDILVAEGSSDSIKPKPLTIFYREKPDAPSCRQDV 61

Query: 105 ITITVPAPFEYK----SSKAVPWRYEYKVTVGQESTSEKKKEKASENKKNNAKEDKKGKA 164
            + ++P     +    +     +  +  +    E+TSEK KEKASE KK   +EDKKGKA
Sbjct: 62  SSPSLPVDNITRVGGLTRTGRCYTPDSLLKRVNETTSEKNKEKASEKKKEKVEEDKKGKA 121

Query: 165 KLNEDVYDELEEAIVVKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSL 224
           KL+EDV+DEL EAIVVKD +PKQPVSEE+TQ+FLKLVKQSEYKVIEQLGRTPAKIS+LSL
Sbjct: 122 KLHEDVHDELVEAIVVKDVSPKQPVSEEETQEFLKLVKQSEYKVIEQLGRTPAKISILSL 181

Query: 225 LLSSEAHQNKLLEVLKQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTL 284
           LLSSEAH+N LLE LKQAFVSQDITVDNL+NVVGNI  SSSITFT+E+IPPEGTGHTK L
Sbjct: 182 LLSSEAHRNALLEALKQAFVSQDITVDNLSNVVGNIMTSSSITFTNEEIPPEGTGHTKAL 241

Query: 285 HITVKYKNFLVAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGD 344
           HI+VK KNFL+AKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S  VGD
Sbjct: 242 HISVKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAFVGD 301

Query: 345 IKIPIQIG 349
           I+IPIQIG
Sbjct: 302 IEIPIQIG 309

[Close]

BLAST of Moc05g16000 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.2e-127
Identity = 258/358 (72.07%), Postives = 286/358 (79.89%), Query Frame = 0


Query: 26  PMPYRAKRHSLKQCNHFQKRVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPK 85
           P    AK HSL+QCN F+ +VQELLD K  TV  SHQKKGIN+VEDVS AE S+ +LKPK
Sbjct: 498 PFHAGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINIVEDVSVAEGSSDALKPK 557

Query: 86  PLTIFYREKPDAPNSSRTLITITVPAPFEYKSSKAVPWRYEYKVTVGQ------------ 145
            LTIFY EKP+APN SR  ITITVPAPFEYKSSKAVPW+Y+ KVTVGQ            
Sbjct: 558 CLTIFYSEKPNAPNCSRKPITITVPAPFEYKSSKAVPWKYQCKVTVGQDVSSPPLPIDNI 617

Query: 146 -----------------------ESTSEKKKEKASENKKNNAKEDKKGKAKLNEDVYDEL 205
                                  E+TSEK KEKASE KK   +EDKKGKAKL+EDV+DEL
Sbjct: 618 TGVGGLTRTGRCYTPDSLLKCVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDEL 677

Query: 206 EEAIVVKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSEAHQNK 265
            EAIVVKD +PKQP+SEE+TQ+ LKLVKQSEYKVIEQLGRTPAKIS+LSLLLSSEAH+N 
Sbjct: 678 VEAIVVKDVSPKQPMSEEETQEILKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAHRNA 737

Query: 266 LLEVLKQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVKYKNFL 325
           LLE LKQAFVSQDITVDNL+NVVGNI+ +SSITFTDE+IPPEGTGHTK LHI++K KNFL
Sbjct: 738 LLEALKQAFVSQDITVDNLSNVVGNISXASSITFTDEEIPPEGTGHTKALHISIKCKNFL 797

Query: 326 VAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPIQIG 349
           +AKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S VVGDI+IPIQIG
Sbjct: 798 IAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQIG 855

[Close]

BLAST of Moc05g16000 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-05
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 1   MMGADRYSWKQQPYGRTPRYAPYYYPMPY 30
           M+GADR+SWKQQPY RTPRY PYYYP PY
Sbjct: 254 MVGADRHSWKQQPYSRTPRYTPYYYPTPY 282

HSP 2 Score: 457.2 bits (1175), Expect = 6.1e-125
Identity = 255/353 (72.24%), Postives = 277/353 (78.47%), Query Frame = 0


Query: 31  AKRHSLKQCNHFQKRVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPKPLTIF 90
           AK HSL+QCN F+ +VQELLD K  T   SH KK  NVVED+  AE S+ SLKPKPLTIF
Sbjct: 541 AKGHSLEQCNXFRMKVQELLDSKILTXANSHXKKXTNVVEDILVAEGSSDSLKPKPLTIF 600

Query: 91  YREKPDAPNSSRTLITITVPAPFEYKSSKAVPWRYEYKVTVGQ----------------- 150
           YREKPDAP+ SR    ITVP PFEYKSSKAVPW+YE KVTVGQ                 
Sbjct: 601 YREKPDAPSCSRKPXXITVPXPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGG 660

Query: 151 ------------------ESTSEKKKEKASENKKNNAKEDKKGKAKLNEDVYDELEEAIV 210
                             E+TSEK KEKASE KK   +EDKKGKAKL+ED  DEL EAIV
Sbjct: 661 LTRTGRCYTPDSLLKRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDARDELVEAIV 720

Query: 211 VKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSEAHQNKLLEVL 270
           VKD +PKQP+SEE+TQ+FLKLVKQSEYKVIEQLGRTPA IS+LSLLLSSEAHQN LLE L
Sbjct: 721 VKDVSPKQPMSEEETQEFLKLVKQSEYKVIEQLGRTPANISILSLLLSSEAHQNALLEAL 780

Query: 271 KQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVKYKNFLVAKVL 330
           KQAFVSQDITVDNL+NVVGNI ASSSI+FTDE+IPPEGTGHTK LHI+VK KNFL+AKVL
Sbjct: 781 KQAFVSQDITVDNLSNVVGNITASSSISFTDEEIPPEGTGHTKALHISVKCKNFLIAKVL 840

Query: 331 VDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPIQIG 349
           VDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S VVGDI+IPIQIG
Sbjct: 841 VDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQIG 893

[Close]

BLAST of Moc05g16000 vs. ExPASy TrEMBL
Match: A0A6J1CNY7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013372 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.9e-125
Identity = 262/363 (72.18%), Postives = 282/363 (77.69%), Query Frame = 0


Query: 26  PMPYRAKRHSLKQCNHFQKRVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNA----- 85
           P    AK H+L+QCN F+  VQELLD K  TV  SHQKKGINVVEDVS AE S A     
Sbjct: 433 PFHAGAKGHALEQCNSFRMIVQELLDSKILTVANSHQKKGINVVEDVSVAEGSIAEGSSD 492

Query: 86  SLKPKPLTIFYREKPDAPNSSRTLITITVPAPFEYKSSKAVPWRYEYKVTVGQ------- 145
           +LKPK LTIFY EKPDAPN SR  ITITVPAPFEYKSSKAVPW+YE KVTVGQ       
Sbjct: 493 ALKPKRLTIFYSEKPDAPNCSRKPITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPPL 552

Query: 146 ----------------------------ESTSEKKKEKASENKKNNAKEDKKGKAKLNED 205
                                       E+TSEK KEKASE KK   +EDKKGKAKL+ED
Sbjct: 553 PVDNITGVGGLTXTGRCYTPDSLLKRVSETTSEKNKEKASEKKKEKVEEDKKGKAKLHED 612

Query: 206 VYDELEEAIVVKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSE 265
           V+DEL EAIVVKD +PKQ V EE+ Q+FLKLVKQSEYKV EQLGRTPAKIS+LSLLLSSE
Sbjct: 613 VHDELVEAIVVKDVSPKQHVFEEEIQEFLKLVKQSEYKVTEQLGRTPAKISILSLLLSSE 672

Query: 266 AHQNKLLEVLKQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVK 325
           AH+N LLE LKQAFVSQDITVDNL+NVVGNI ASSSITFTDE+IPPEGTGHTK LHI+VK
Sbjct: 673 AHRNTLLEXLKQAFVSQDITVDNLSNVVGNITASSSITFTDEEIPPEGTGHTKALHISVK 732

Query: 326 YKNFLVAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPI 349
            KNFL+AKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S VVGDI+IPI
Sbjct: 733 CKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPI 792

[Close]

BLAST of Moc05g16000 vs. ExPASy TrEMBL
Match: A0A6J1D7C7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111018303 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.1e-110
Identity = 235/339 (69.32%), Postives = 257/339 (75.81%), Query Frame = 0


Query: 45  RVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPKPLTIFYREKPDAPNSSRTL 104
           +VQELLD K  TV  SHQKK  NVVED+  AE S+ S+KPK LTIFYREKPDAP+ SR  
Sbjct: 2   KVQELLDSKILTVANSHQKKRTNVVEDILVAEGSSDSIKPKRLTIFYREKPDAPSCSRKP 61

Query: 105 ITITVPAPFEYKSSKAVPWRYEYKVTVGQ------------------------------- 164
           ITITVPAPFEYKSSKAVPW+YE KVTVGQ                               
Sbjct: 62  ITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLL 121

Query: 165 ----ESTSEKKKEKASENKKNNAKEDKKGKAKLNEDVYDELEEAIVVKDANPKQPVSEED 224
               E+TSEK KEKASE KK   +EDKKGKAKL+EDV+DEL                EE+
Sbjct: 122 KRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDEL---------------VEEE 181

Query: 225 TQKFLKLVKQSEYKVIEQLGRTPAKISLLSLLLSSEAHQNKLLEVLKQAFVSQDITVDNL 284
           TQ+FLKLVKQ+EYKVIEQLGRTPAKIS+LSLLLSSEAH+N LLE LKQAFVSQDITVDNL
Sbjct: 182 TQEFLKLVKQNEYKVIEQLGRTPAKISILSLLLSSEAHRNALLEALKQAFVSQDITVDNL 241

Query: 285 NNVVGNIAASSSITFTDEKIPPEGTGHTKTLHITVKYKNFLVAKVLVDNGSSLNIMPRST 344
           +NVVGNI ASS ITFTDE+IPPEGTGHTK LHI+VK KNFL+AKVLV NGSSLNIMPRST
Sbjct: 242 SNVVGNIMASSCITFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVGNGSSLNIMPRST 301

Query: 345 LEKLPVDMSHMRPSTMIVTAFDGACSTVVGDIKIPIQIG 349
           LEKLPVDMSHMRPST+IV AFDGA + VVGDI+IPIQIG
Sbjct: 302 LEKLPVDMSHMRPSTVIVRAFDGARNAVVGDIEIPIQIG 325

[Close]

BLAST of Moc05g16000 vs. ExPASy TrEMBL
Match: A0A6J1DR66 (uncharacterized protein LOC111022791 OS=Momordica charantia OX=3673 GN=LOC111022791 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 1.3e-106
Identity = 222/308 (72.08%), Postives = 253/308 (82.14%), Query Frame = 0


Query: 45  RVQELLDIKAFTVTKSHQKKGINVVEDVSTAESSNASLKPKPLTIFYREKPDAPNSSRTL 104
           +VQELLD K  TV  SHQKK  NVVED+  AE S+ S+KPKPLTIFYREKPDAP+  + +
Sbjct: 2   KVQELLDSKILTVANSHQKKRTNVVEDILVAEGSSDSIKPKPLTIFYREKPDAPSCRQDV 61

Query: 105 ITITVPAPFEYK----SSKAVPWRYEYKVTVGQESTSEKKKEKASENKKNNAKEDKKGKA 164
            + ++P     +    +     +  +  +    E+TSEK KEKASE KK   +EDKKGKA
Sbjct: 62  SSPSLPVDNITRVGGLTRTGRCYTPDSLLKRVNETTSEKNKEKASEKKKEKVEEDKKGKA 121

Query: 165 KLNEDVYDELEEAIVVKDANPKQPVSEEDTQKFLKLVKQSEYKVIEQLGRTPAKISLLSL 224
           KL+EDV+DEL EAIVVKD +PKQPVSEE+TQ+FLKLVKQSEYKVIEQLGRTPAKIS+LSL
Sbjct: 122 KLHEDVHDELVEAIVVKDVSPKQPVSEEETQEFLKLVKQSEYKVIEQLGRTPAKISILSL 181

Query: 225 LLSSEAHQNKLLEVLKQAFVSQDITVDNLNNVVGNIAASSSITFTDEKIPPEGTGHTKTL 284
           LLSSEAH+N LLE LKQAFVSQDITVDNL+NVVGNI  SSSITFT+E+IPPEGTGHTK L
Sbjct: 182 LLSSEAHRNALLEALKQAFVSQDITVDNLSNVVGNIMTSSSITFTNEEIPPEGTGHTKAL 241

Query: 285 HITVKYKNFLVAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTMIVTAFDGACSTVVGD 344
           HI+VK KNFL+AKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPST+IV AFDGA S  VGD
Sbjct: 242 HISVKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAFVGD 301

Query: 345 IKIPIQIG 349
           I+IPIQIG
Sbjct: 302 IEIPIQIG 309

The following BLAST results are available for this feature:

BLAST of Moc05g16000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158986.1	4.6e-127	72.07	LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia]	[more]
XP_022158986.1	5.8e-05	82.76	LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia]	[more]
XP_022143495.1	1.6e-124	72.18	LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia]	[more]
XP_022150030.1	2.3e-110	69.32	LOW QUALITY PROTEIN: uncharacterized protein LOC111018303 [Momordica charantia]	[more]
XP_022155744.1	2.6e-106	72.08	uncharacterized protein LOC111022791 [Momordica charantia]	[more]

BLAST of Moc05g16000 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc05g16000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E2J7	2.2e-127	72.07	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1	[more]
A0A6J1E2J7	2.8e-05	82.76	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1	[more]
A0A6J1CNY7	7.9e-125	72.18	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013372 PE=4 SV=1	[more]
A0A6J1D7C7	1.1e-110	69.32	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111018303 PE=4 SV=1	[more]
A0A6J1DR66	1.3e-106	72.08	uncharacterized protein LOC111022791 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]

BLAST of Moc05g16000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 262..349 e-value: 4.3E-5 score: 25.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 134..160
None	No IPR available	PANTHER	PTHR33240:SF7	GAG-PRO-LIKE PROTEIN	coord: 24..348
None	No IPR available	PANTHER	PTHR33240	OS08G0508500 PROTEIN	coord: 24..348
None	No IPR available	CDD	cd00303	retropepsin_like	coord: 282..349 e-value: 4.28497E-8 score: 48.4868

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g16000.1	Moc05g16000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0015074	DNA integration
biological_process	GO:0006310	DNA recombination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity