Moc05g15400 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g15400
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease H
Locationchr5: 11669118 .. 11672167 (+)
RNA-Seq ExpressionMoc05g15400
SyntenyMoc05g15400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAAGTATGAGGGCCGAGGTGGACTTGGCCAAGGTCCGATCTACCGGGAAGCTCGGTGGGGGCCGATGTGAGCTACTGTCAGCGGCGTGATTATCGGGTGTAACCGTCGGTCCCGAGATTATCGGGTGTAACCGACGGTCCCGAGATTACCGGGTGTAACCGCCGCGAGATTAGCGGGACTACCACCTATAAGTAGAGGACCTCACTCCACGATCAGGTACGATGATTCTTTGACCTCGAACTAAATTGGGAATCCGACCTATACTGACTTGATCGTCGGAGTGCTCACCTTTTTGTGCAGGTCCGCCCAAGTATTCAGATCGGTCCGGAGACCGAGTTCGAGCTGTAATCAGAAATACACTGTTGTGCATATCCTTGCATAAACAAAATATATAAATTATTAATTAATAGTAATTAATAGTAAGTGACAATGTTATCTAAATTTAAAATTTTACGAAGGATAAAATAGTCAAAACATGTATTTTTAAAGATGGTAACTTTTTATATAAATACGATGGTAAAATATAAATTTGGACCAGTTCAATATTTGGTCTTTATTATCAAGGGTCTTTTTGAAAGTTTTAAAAAATCTTTCAAAAATATATAATAGGTTTGTCCATAGATTTTTTCAATGGGTCAATCTTAAAAAAAAAAAAAAACTTCCATTATGATGTTTTTACATGTAAAATTGAATGAGACCAATAATTTGAAATTGAACCAACCAAGTTGGTTTAGTCAATTAATTTCGTTGTTGAATCAAACCGTTTAAAACCATTTGTACGTTGAACGTATTGAATAGTCTATATAATTTTTATGATTATTTTAATTCTGAAAACGTAAGTGTATATTTGATAGTGATGGAAGCATGGAGTGGAACGACAATTTCACTCTTTGGCTTAAAGATGTAGTTCTTTCAAAATTTTCCCTTTGTTAGTTTCTGTTTTGTATTGCTAAGAAAAAAAATGTATATTTGATATTTTAATCATTTTAAAGTAGGTTCATGCTTTTTAAAAAATAATAATAATAAAGAAAACTAATCACATTTTTTATTAAAGGAATAAAATTCAATGTCAAGACGAAGGTAGGAGGCATTATGGAGTTTGAATTCATTCCCTTTTAATTAACTCTAGCTCTTTTGGACCAATGAGTCACCCAAGATGGTTAATAATTCATACTTTTCACTCAACCCAACCTCTATCAACAAGCAACCTCATGAGCCCGTACAACCAGCTGTTGATCACTACCTACTCAACTTTCCATGTACATCACACTTAATGACAGATCCATAAATTTTGTTGAGACGGGCTTCACACTAATATACAAGTAAATAATTTCAAGAATTTATAAAATTATGATTTTGAGACTAACATAATCATTTTTGTAAATATGCTCTACGAATTTGGTCTCGAATATTACAATTGTAATAAACAATTTTAGTTCTTAGACTGAGATCTGTCGGTAATTTTCTTAACTTACTTATGTAAAATATCAATCAGAACTACTATTACTGAGACGATGATCTCATCCATTCTCTTTGTAGTATCTCTCACATTTAAATTAAATCTACAATAGCATTAATAAAAAAAAAATACCAAGGTGCAGGCTCAAACTCAAAACCGTTAGAGGGGCTAGTGCGTAGGCATTATGCCAAAGATCTATTTGATTCTTAAGCTGGACTTTTATATACTAATTTTTAGTTCCAAAAGTTCGCGCTCCTGATTGCACCTATTATTTTACCAGTCTGATTTATCTCCCTTACCTAGCAAACATATGGATACATTTAGGCTATGTTTACTTCAACGGAATGAAAATCAAGGAATTTGAATCAATGCCAACATGGACTTAGCTTAGCGGTAATTGACATCTGTCTTTGACCAAGAGGTCATAAGTTCGAATCTTCTCTCCTCCTTATTGTATTAAAAGAAAATGTGAATCAATTTTTCATTCCCTTATACTCACCATGTCCACATTGTTGCTTGTCGTAAAGTGTTCGCTGCTACTGCCTATCGGTGCTAGTCGCTACTGCTGTTATTCATGATCACTTTTTTGTTGCAAACAATAAAGAAAATGAAAAGGATTTTTTGTGAGGAGAGGGTACACAATTCCTGGTAGATAAAAATGCGAACAAATCGAGAAAGATAGAGATTCACCTTTGTTTGGGGGTAAACATACCCAGGACACGTATAAATAGAGGACTTCATTTCACGCTCAGGTATCGAATCTCACCTCGAACTAAATAAGGAGTCCGATCTATACTGACTTGGGCGTCGGAGTGCTTACCCTCTTGTGCAGGTCCACTCTAGTGTTCAAGTCGGAACCGGAGACCGGGTTCGAGCTCGATTCGTAAAGAACCGTTGTGCAAATTCCTGCATAAACATTTGGCGTCGTCTGTGGGGAAGGACGATCTAAGTCATCCCGATCTAGAAAATATACGCAACGATGGTGCATCCGGCAAGCTCTGCTAATACGACAGAACGGAGGGGTGTGAACGCTGATAATGGCACTCAGCGAGGCATCGACGCAAGAATAGTCGAGGATCAGGTCCGAGCAGGGCAAAAGGGAGATCTGCCCGCCGTGCGAACCAAGAGTTACCCCTTGCTCATCCGAAACCCTCAAAGGCCAACCATGGCCGAGGAGGGACCTCGAGAAAAACCTCCCAAAGGGCCAACCAGGCAGACCCTGAAGCTTTGACTACTCTCCAGCGCGAGTTGGATGATATGCGCCATCTGTTGCGCACAATAGAAGAGATGTACGCCGAGGCAACGCGTGCTAACCGAACCGCGTCTCCCTCTAGGGCCCCTGGAGCACCCGGTGAAAAAGGAGCTCCGTCTATCCAACCTGGCGACCGCGAGCCCATCCCCAACGATGGAGTGGATTACAGCTTGCGGGATAACGATCTAAGAAAGCACCTGACTGAAAAGAAGAAGAAAGCCTCTTGGGTGCCGGAGGACTCACCGTCCTACTCCCGAGAGTTCTCCAACTCTGACCTTAAGGCTCAGTCAAAGTATAAGCCCCTAACACCAGAAGCTGTGATCACCAGGGAATAG

mRNA sequence

ATGCTAAGTATGAGGGCCGAGGTGGACTTGGCCAAGGTCCGATCTACCGGGAAGCTCGGTGGGGGCCGATGTCCGCCCAAGTATTCAGATCGGTCCGGAGACCGAGTTCGAGCTGTCCACTCTAGTGTTCAAGTCGGAACCGGAGACCGGGTTCGAGCTCGATTCGTAAAGAACCGTTCGAGGCATCGACGCAAGAATAGTCGAGGATCAGGTCCGAGCAGGGCAAAAGGGAGATCTGCCCGCCGTGCGAACCAAGAGTTACCCCTTGCTCATCCGAAACCCTCAAAGGCCAACCATGGCCGAGGAGGGACCTCGAGAAAAACCTCCCAAAGGGCCAACCAGGCAGACCCTGAAGCTTTGACTACTCTCCAGCGCGAGTTGGATGATATGCGCCATCTGTTGCGCACAATAGAAGAGATGTACGCCGAGGCAACGCGTGCTAACCGAACCGCGTCTCCCTCTAGGGCCCCTGGAGCACCCGGTGAAAAAGGAGCTCCGTCTATCCAACCTGGCGACCGCGAGCCCATCCCCAACGATGGAGTGGATTACAGCTTGCGGGATAACGATCTAAGAAAGCACCTGACTGAAAAGAAGAAGAAAGCCTCTTGGGTGCCGGAGGACTCACCGTCCTACTCCCGAGAGTTCTCCAACTCTGACCTTAAGGCTCAGTCAAAGTATAAGCCCCTAACACCAGAAGCTGTGATCACCAGGGAATAG

Coding sequence (CDS)

ATGCTAAGTATGAGGGCCGAGGTGGACTTGGCCAAGGTCCGATCTACCGGGAAGCTCGGTGGGGGCCGATGTCCGCCCAAGTATTCAGATCGGTCCGGAGACCGAGTTCGAGCTGTCCACTCTAGTGTTCAAGTCGGAACCGGAGACCGGGTTCGAGCTCGATTCGTAAAGAACCGTTCGAGGCATCGACGCAAGAATAGTCGAGGATCAGGTCCGAGCAGGGCAAAAGGGAGATCTGCCCGCCGTGCGAACCAAGAGTTACCCCTTGCTCATCCGAAACCCTCAAAGGCCAACCATGGCCGAGGAGGGACCTCGAGAAAAACCTCCCAAAGGGCCAACCAGGCAGACCCTGAAGCTTTGACTACTCTCCAGCGCGAGTTGGATGATATGCGCCATCTGTTGCGCACAATAGAAGAGATGTACGCCGAGGCAACGCGTGCTAACCGAACCGCGTCTCCCTCTAGGGCCCCTGGAGCACCCGGTGAAAAAGGAGCTCCGTCTATCCAACCTGGCGACCGCGAGCCCATCCCCAACGATGGAGTGGATTACAGCTTGCGGGATAACGATCTAAGAAAGCACCTGACTGAAAAGAAGAAGAAAGCCTCTTGGGTGCCGGAGGACTCACCGTCCTACTCCCGAGAGTTCTCCAACTCTGACCTTAAGGCTCAGTCAAAGTATAAGCCCCTAACACCAGAAGCTGTGATCACCAGGGAATAG

Protein sequence

MLSMRAEVDLAKVRSTGKLGGGRCPPKYSDRSGDRVRAVHSSVQVGTGDRVRARFVKNRSRHRRKNSRGSGPSRAKGRSARRANQELPLAHPKPSKANHGRGGTSRKTSQRANQADPEALTTLQRELDDMRHLLRTIEEMYAEATRANRTASPSRAPGAPGEKGAPSIQPGDREPIPNDGVDYSLRDNDLRKHLTEKKKKASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE
Homology
[Close]
BLAST of Moc05g15400 vs. NCBI nr
Match: XP_022154406.1 (uncharacterized protein LOC111021683 [Momordica charantia])

HSP 1 Score: 171.0 bits (432), Expect = 1.2e-38
Identity = 89/103 (86.41%), Postives = 97/103 (94.17%), Query Frame = 0 

Query: 137 IEEMYAEATRANRTASPSRAPGAPGEKGAPSIQPGDREPIPND-GVDYSLRDNDLRKHLT 196
           +E+MYAEATRANRTASPS APGAPGEKGAPSIQPGDREPIPND G+DYSLRDND+RKHLT
Sbjct: 1   MEKMYAEATRANRTASPSIAPGAPGEKGAPSIQPGDREPIPNDGGMDYSLRDNDMRKHLT 60

Query: 197 EKKKKASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           EKKK+AS  PEDS SYSR+FSNS+LKAQSKYKPL PEA+ITRE
Sbjct: 61  EKKKRASREPEDSLSYSRKFSNSNLKAQSKYKPLAPEAMITRE 103

[Close]
BLAST of Moc05g15400 vs. NCBI nr
Match: XP_022155139.1 (uncharacterized protein LOC111022280 [Momordica charantia])

HSP 1 Score: 146.7 bits (369), Expect = 2.5e-31
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 0 

Query: 157 PGAPGEKGAPSIQPGDREPIPND-GVDYSLRDNDLRKHLTEKKKKASWVPEDSPSYSREF 216
           PGAPGEKGAPSIQPG+REPIPND GVDYSLRDNDLRKHLT+KKKKASW PEDS SYSREF
Sbjct: 5   PGAPGEKGAPSIQPGNREPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREF 64

Query: 217 SNSDLKAQSKYKPLTPEAVITRE 239
           SNS+LKAQSKYKPL PEAVI RE
Sbjct: 65  SNSNLKAQSKYKPLIPEAVINRE 87

[Close]
BLAST of Moc05g15400 vs. NCBI nr
Match: XP_022150385.1 (uncharacterized protein LOC111018561 [Momordica charantia])

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-10
Identity = 42/53 (79.25%), Postives = 46/53 (86.79%), Query Frame = 0 

Query: 186 RDNDLRKHLTEKKKKASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           RD D R  + ++KK+AS  PEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE
Sbjct: 24  RDLDARM-VEDQKKRASREPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 75

[Close]
BLAST of Moc05g15400 vs. NCBI nr
Match: XP_022159327.1 (uncharacterized protein LOC111025738 [Momordica charantia])

HSP 1 Score: 75.9 bits (185), Expect = 5.4e-10
Identity = 64/162 (39.51%), Postives = 83/162 (51.23%), Query Frame = 0 

Query: 78  RSARRANQELPLAHPKPSKANHGRGGTSRKTSQRANQA-DPEALTTLQRELDDMRHLLRT 137
           RSAR    +L  AHPKP KAN GRGG SR+T+  A  A   E    LQ+E++ MR  + T
Sbjct: 46  RSARITTPDLSPAHPKPFKANRGRGGASRRTTLGAAPAPSRENFDALQKEMEAMRTQMLT 105

Query: 138 IEEMYAEATRANRTASPSRAPGAPGEKGAPSIQPGDREPIPNDGVDYSLRDNDLRKHLTE 197
           +EEMY E  +A    S S    A  E+G                        DLR HL+ 
Sbjct: 106 MEEMYNEMVQAVGAGSRSEDRAARDERG------------------------DLRDHLS- 165

Query: 198 KKKKASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           +K+ +S     SPS S +  NS+ +A+S Y P+ PE VITRE
Sbjct: 166 RKRSSSLRKGRSPSCSHK--NSNQQAESSYNPVVPEGVITRE 180

[Close]
BLAST of Moc05g15400 vs. NCBI nr
Match: XP_022157897.1 (uncharacterized protein LOC111024503 [Momordica charantia])

HSP 1 Score: 67.4 bits (163), Expect = 1.9e-07
Identity = 34/39 (87.18%), Postives = 35/39 (89.74%), Query Frame = 0 

Query: 200 KASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           +AS   EDSPSYSREFSNSD KAQSKYKPLTPEAVITRE
Sbjct: 468 RASRESEDSPSYSREFSNSDFKAQSKYKPLTPEAVITRE 506

[Close]
BLAST of Moc05g15400 vs. ExPASy TrEMBL
Match: A0A6J1DNM4 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021683 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 5.9e-39
Identity = 89/103 (86.41%), Postives = 97/103 (94.17%), Query Frame = 0 

Query: 137 IEEMYAEATRANRTASPSRAPGAPGEKGAPSIQPGDREPIPND-GVDYSLRDNDLRKHLT 196
           +E+MYAEATRANRTASPS APGAPGEKGAPSIQPGDREPIPND G+DYSLRDND+RKHLT
Sbjct: 1   MEKMYAEATRANRTASPSIAPGAPGEKGAPSIQPGDREPIPNDGGMDYSLRDNDMRKHLT 60

Query: 197 EKKKKASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           EKKK+AS  PEDS SYSR+FSNS+LKAQSKYKPL PEA+ITRE
Sbjct: 61  EKKKRASREPEDSLSYSRKFSNSNLKAQSKYKPLAPEAMITRE 103

[Close]
BLAST of Moc05g15400 vs. ExPASy TrEMBL
Match: A0A6J1DPC9 (uncharacterized protein LOC111022280 OS=Momordica charantia OX=3673 GN=LOC111022280 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.2e-31
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 0 

Query: 157 PGAPGEKGAPSIQPGDREPIPND-GVDYSLRDNDLRKHLTEKKKKASWVPEDSPSYSREF 216
           PGAPGEKGAPSIQPG+REPIPND GVDYSLRDNDLRKHLT+KKKKASW PEDS SYSREF
Sbjct: 5   PGAPGEKGAPSIQPGNREPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREF 64

Query: 217 SNSDLKAQSKYKPLTPEAVITRE 239
           SNS+LKAQSKYKPL PEAVI RE
Sbjct: 65  SNSNLKAQSKYKPLIPEAVINRE 87

[Close]
BLAST of Moc05g15400 vs. ExPASy TrEMBL
Match: A0A6J1D8C0 (uncharacterized protein LOC111018561 OS=Momordica charantia OX=3673 GN=LOC111018561 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 6.8e-11
Identity = 42/53 (79.25%), Postives = 46/53 (86.79%), Query Frame = 0 

Query: 186 RDNDLRKHLTEKKKKASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           RD D R  + ++KK+AS  PEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE
Sbjct: 24  RDLDARM-VEDQKKRASREPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 75

[Close]
BLAST of Moc05g15400 vs. ExPASy TrEMBL
Match: A0A6J1DZJ1 (uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025738 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.6e-10
Identity = 64/162 (39.51%), Postives = 83/162 (51.23%), Query Frame = 0 

Query: 78  RSARRANQELPLAHPKPSKANHGRGGTSRKTSQRANQA-DPEALTTLQRELDDMRHLLRT 137
           RSAR    +L  AHPKP KAN GRGG SR+T+  A  A   E    LQ+E++ MR  + T
Sbjct: 46  RSARITTPDLSPAHPKPFKANRGRGGASRRTTLGAAPAPSRENFDALQKEMEAMRTQMLT 105

Query: 138 IEEMYAEATRANRTASPSRAPGAPGEKGAPSIQPGDREPIPNDGVDYSLRDNDLRKHLTE 197
           +EEMY E  +A    S S    A  E+G                        DLR HL+ 
Sbjct: 106 MEEMYNEMVQAVGAGSRSEDRAARDERG------------------------DLRDHLS- 165

Query: 198 KKKKASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           +K+ +S     SPS S +  NS+ +A+S Y P+ PE VITRE
Sbjct: 166 RKRSSSLRKGRSPSCSHK--NSNQQAESSYNPVVPEGVITRE 180

[Close]
BLAST of Moc05g15400 vs. ExPASy TrEMBL
Match: A0A6J1DVR0 (uncharacterized protein LOC111024503 OS=Momordica charantia OX=3673 GN=LOC111024503 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 9.2e-08
Identity = 34/39 (87.18%), Postives = 35/39 (89.74%), Query Frame = 0 

Query: 200 KASWVPEDSPSYSREFSNSDLKAQSKYKPLTPEAVITRE 239
           +AS   EDSPSYSREFSNSD KAQSKYKPLTPEAVITRE
Sbjct: 468 RASRESEDSPSYSREFSNSDFKAQSKYKPLTPEAVITRE 506

The following BLAST results are available for this feature:
BLAST of Moc05g15400 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022154406.11.2e-3886.41uncharacterized protein LOC111021683 [Momordica charantia][more]
XP_022155139.12.5e-3190.36uncharacterized protein LOC111022280 [Momordica charantia][more]
XP_022150385.11.4e-1079.25uncharacterized protein LOC111018561 [Momordica charantia][more]
XP_022159327.15.4e-1039.51uncharacterized protein LOC111025738 [Momordica charantia][more]
XP_022157897.11.9e-0787.18uncharacterized protein LOC111024503 [Momordica charantia][more]
BLAST of Moc05g15400 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Moc05g15400 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DNM45.9e-3986.41Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021683 PE=4 SV=1[more]
A0A6J1DPC91.2e-3190.36uncharacterized protein LOC111022280 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1D8C06.8e-1179.25uncharacterized protein LOC111018561 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1DZJ12.6e-1039.51uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DVR09.2e-0887.18uncharacterized protein LOC111024503 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
BLAST of Moc05g15400 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 120..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 209..224
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 144..238
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g15400.1Moc05g15400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity