Moc05g14880 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g14880
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionReverse transcriptase
Locationchr5: 11223891 .. 11224962 (+)
RNA-Seq ExpressionMoc05g14880
SyntenyMoc05g14880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACAAGCTTGGTGCAATTTGCTTCGGGACAAGATTCGGGACATATCCCAGACTGATCTCGAACCAAGATTGGTCTGAGACATGTCTCGGACTAATCCTGAACCATGATTGGTCCGGGACAGTTCGAGATTAATTAAATTTTTTTGGATTATTTTTTTAATGTTTTTTTGTTAATCATTTTCTTATCTCACCTAATTTTATTCTATTTGCAAGATGCCTCGTGTTTTCATGACATTCGATGGATAGCGGAATGATAGTGAAAAAGATTATGTCGGTGGTCGTACGAGAGGATTGACAGTAGATACTACAACCACATACAAAGAATTTCTAGGTCATGTATATAGATTGAGTAATATAAACCCCTTATAGTTTGTTATTATAATTAGACTTGTATATCATTTTAAAACTAACGTTTGTGAAATGGAAATAATTGATGACAATGACCTGAGTTTTTTTTATTAGCGAGGATGTATCTGAATTGCATGTATTCATATCTACTGTGTCGAAGCAAGTACATGGAAATGAAATGTAGATGCATTCTTTCCCATATTATGTAGGCCAACACGTGTCATCCATTCCTGTGCCCACAACTTGTATCCCCCCAATTCCAAAAACCCACATATCGTAGACACTCATTTCCTTCCTCATCATCGAACCTCTCTTCTTCTGAACCCTCACATCCCTCCTACGGACATATAGGTCATGATATAGCATCTTTAACACCATTGGATTCAGATCATGTTGTTCCATGTAATCTGAGGGATGAAAGGGTATGTGATTGGGATGCGTCGAATGATAATGAAGATGAAAGTGATGAATCATATTTCCCGTTGGGAAAGACTGAAGAAGGAGACTCTGAAGCAGAATATGGGAATGATGCCAATGACTATGCACTTGATGAAGACCATGATGTGCAACAGGTAAACAATGGGATTAGTAGGGATGAAGAAGCGGTCCGACAAACAGTATGTAATGGTCTCTCCGGACAACCTAATGATGAGAAATTGCAACTCATGGTACAGTCTTTTAGGACAAATGATGTTAAGGAGAGCAAAGTATTTGATACGTAG

mRNA sequence

ATGTACAAGCTTGGTGCAATTTGCTTCGGGACAAGATTCGGGACATATCCCAGACTGATCTCGAACCAAGATTGGTCTGAGACATGTCTCGGACTAATCCTGAACCATGATTGGTCCGGGACAGCCAACACGTGTCATCCATTCCTGTGCCCACAACTTGTATCCCCCCAATTCCAAAAACCCACATATCGTAGACACTCATTTCCTTCCTCATCATCGAACCTCTCTTCTTCTGAACCCTCACATCCCTCCTACGGACATATAGGTCATGATATAGCATCTTTAACACCATTGGATTCAGATCATGTTGTTCCATGTAATCTGAGGGATGAAAGGGTATGTGATTGGGATGCGTCGAATGATAATGAAGATGAAAGTGATGAATCATATTTCCCGTTGGGAAAGACTGAAGAAGGAGACTCTGAAGCAGAATATGGGAATGATGCCAATGACTATGCACTTGATGAAGACCATGATGTGCAACAGGTAAACAATGGGATTAGTAGGGATGAAGAAGCGGTCCGACAAACAGTATGTAATGGTCTCTCCGGACAACCTAATGATGAGAAATTGCAACTCATGGTACAGTCTTTTAGGACAAATGATGTTAAGGAGAGCAAAGTATTTGATACGTAG

Coding sequence (CDS)

ATGTACAAGCTTGGTGCAATTTGCTTCGGGACAAGATTCGGGACATATCCCAGACTGATCTCGAACCAAGATTGGTCTGAGACATGTCTCGGACTAATCCTGAACCATGATTGGTCCGGGACAGCCAACACGTGTCATCCATTCCTGTGCCCACAACTTGTATCCCCCCAATTCCAAAAACCCACATATCGTAGACACTCATTTCCTTCCTCATCATCGAACCTCTCTTCTTCTGAACCCTCACATCCCTCCTACGGACATATAGGTCATGATATAGCATCTTTAACACCATTGGATTCAGATCATGTTGTTCCATGTAATCTGAGGGATGAAAGGGTATGTGATTGGGATGCGTCGAATGATAATGAAGATGAAAGTGATGAATCATATTTCCCGTTGGGAAAGACTGAAGAAGGAGACTCTGAAGCAGAATATGGGAATGATGCCAATGACTATGCACTTGATGAAGACCATGATGTGCAACAGGTAAACAATGGGATTAGTAGGGATGAAGAAGCGGTCCGACAAACAGTATGTAATGGTCTCTCCGGACAACCTAATGATGAGAAATTGCAACTCATGGTACAGTCTTTTAGGACAAATGATGTTAAGGAGAGCAAAGTATTTGATACGTAG

Protein sequence

MYKLGAICFGTRFGTYPRLISNQDWSETCLGLILNHDWSGTANTCHPFLCPQLVSPQFQKPTYRRHSFPSSSSNLSSSEPSHPSYGHIGHDIASLTPLDSDHVVPCNLRDERVCDWDASNDNEDESDESYFPLGKTEEGDSEAEYGNDANDYALDEDHDVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLMVQSFRTNDVKESKVFDT
Homology
BLAST of Moc05g14880 vs. NCBI nr
Match: XP_022155970.1 (uncharacterized protein LOC111022954 [Momordica charantia])

HSP 1 Score: 181.8 bits (460), Expect = 6.1e-42
Identity = 110/183 (60.11%), Postives = 126/183 (68.85%), Query Frame = 0

Query: 34  LNHDWSGTANTCHPFLCPQLVSPQFQKPTYRRHSFPSSSSNLSSSEPSHPSYGHIGHDIA 93
           L  D    +++C P  C     P FQ+PT    SFPSSSSN SSS+  H  YGH+GHDIA
Sbjct: 61  LTVDSQNVSSSCMPIAC----IPSFQRPTDPIPSFPSSSSNPSSSQQPHLYYGHLGHDIA 120

Query: 94  SLTPLDSDHVVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEYGNDAN 153
            LTPL+SD VVPCNL D+RVC W+     NDN+DESDESY  LGK+EEGD EAE+ ND  
Sbjct: 121 GLTPLESD-VVPCNLGDDRVCVWNMPGLWNDNQDESDESYDSLGKSEEGDYEAEFMNDDY 180

Query: 154 DYALDE--DHDVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLMVQSFRTNDVKESKV 212
           D A DE  + DV+QV   I RDE  V Q  C+GL GQPNDEKLQL+VQS  TNDVKE KV
Sbjct: 181 DDARDEKFEPDVEQVQVEIRRDEVVVLQMGCDGLIGQPNDEKLQLIVQSSGTNDVKEGKV 238

BLAST of Moc05g14880 vs. NCBI nr
Match: XP_022157017.1 (uncharacterized protein LOC111023843 [Momordica charantia])

HSP 1 Score: 149.1 bits (375), Expect = 4.4e-32
Identity = 83/127 (65.35%), Postives = 95/127 (74.80%), Query Frame = 0

Query: 89  GHDIASLTPLDSDHVVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEY 148
           GHD+  LTPL SD VVPCNL D+RVCDWD     NDNEDES ESY PL  +EEG S+AEY
Sbjct: 8   GHDVEGLTPLGSD-VVPCNLGDDRVCDWDVPGVWNDNEDESGESYDPLAXSEEGHSQAEY 67

Query: 149 GNDANDYALDE--DHDVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLMVQSFRTNDV 208
           GN+ +D ALD+  + DV+QV+  I RDEEAVR   CNGL+G PNDEKLQL+VQS  TNDV
Sbjct: 68  GNEEHDDALDDELEXDVEQVHTEIRRDEEAVRXPGCNGLTGDPNDEKLQLIVQSSGTNDV 127

Query: 209 KESKVFD 211
            E  VFD
Sbjct: 128 NEGDVFD 133

BLAST of Moc05g14880 vs. NCBI nr
Match: XP_022156328.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111023249 [Momordica charantia])

HSP 1 Score: 133.3 bits (334), Expect = 2.5e-27
Identity = 73/114 (64.04%), Postives = 87/114 (76.32%), Query Frame = 0

Query: 102  HVVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEYGNDANDYALDE-- 161
            +VVPCNL D+RVCDWD     NDNEDES ESY PL +++EG S+AEYGN+ +D ALD+  
Sbjct: 1132 NVVPCNLGDDRVCDWDVPGVWNDNEDESGESYDPLAESKEGHSQAEYGNEEHDDALDDEL 1191

Query: 162  DHDVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLMVQSFRTNDVKESKVFD 211
            + DV+QV+  I RDEEAVR   CNGL+G PNDEKLQL+VQS  TNDV E  VFD
Sbjct: 1192 EPDVEQVHTEIRRDEEAVRPPGCNGLTGDPNDEKLQLIVQSSGTNDVNEGDVFD 1245

BLAST of Moc05g14880 vs. NCBI nr
Match: XP_022154947.1 (uncharacterized protein LOC111022090 [Momordica charantia])

HSP 1 Score: 132.5 bits (332), Expect = 4.3e-27
Identity = 78/126 (61.90%), Postives = 92/126 (73.02%), Query Frame = 0

Query: 43  NTCHPFLCPQLVSPQFQKPTYRRHSFPSSSSNLSSSEPSHPSYGHIGHDIASLTPLDSDH 102
           ++C P  C     PQFQ+PT    SFPSSSSN SSS+  HP Y H+GHDIA LTPL+SD 
Sbjct: 45  SSCMPTTC----IPQFQRPTDPIPSFPSSSSNPSSSQQPHPYYRHLGHDIAGLTPLESD- 104

Query: 103 VVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEYGNDANDYALDEDH- 162
           VVPCNL D+RVCDW+     NDN+DESDESY PLG++EEGD EAE+ ND  D ALDE+  
Sbjct: 105 VVPCNLGDDRVCDWNVPGLWNDNQDESDESYDPLGESEEGDYEAEFVNDDYDDALDEESE 164

Query: 163 -DVQQV 164
            DV+QV
Sbjct: 165 PDVEQV 165

BLAST of Moc05g14880 vs. NCBI nr
Match: XP_022153146.1 (uncharacterized protein LOC111020715 [Momordica charantia])

HSP 1 Score: 78.6 bits (192), Expect = 7.3e-11
Identity = 48/77 (62.34%), Postives = 57/77 (74.03%), Query Frame = 0

Query: 137 EEGDSEAEYGNDANDYALDEDH--DVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLM 196
           EEGD EAE+ ND  D ALDE+   DV+QV+  I RDE AV+Q  C+GL+GQ N E LQL+
Sbjct: 3   EEGDYEAEFVNDDYDDALDEESEPDVEQVHAEICRDEAAVQQMGCDGLTGQHNYENLQLI 62

Query: 197 VQSFRTNDVKESKVFDT 212
           VQS  TNDVKE +VFDT
Sbjct: 63  VQSSGTNDVKEGEVFDT 79

BLAST of Moc05g14880 vs. ExPASy TrEMBL
Match: A0A6J1DP00 (uncharacterized protein LOC111022954 OS=Momordica charantia OX=3673 GN=LOC111022954 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 3.0e-42
Identity = 110/183 (60.11%), Postives = 126/183 (68.85%), Query Frame = 0

Query: 34  LNHDWSGTANTCHPFLCPQLVSPQFQKPTYRRHSFPSSSSNLSSSEPSHPSYGHIGHDIA 93
           L  D    +++C P  C     P FQ+PT    SFPSSSSN SSS+  H  YGH+GHDIA
Sbjct: 61  LTVDSQNVSSSCMPIAC----IPSFQRPTDPIPSFPSSSSNPSSSQQPHLYYGHLGHDIA 120

Query: 94  SLTPLDSDHVVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEYGNDAN 153
            LTPL+SD VVPCNL D+RVC W+     NDN+DESDESY  LGK+EEGD EAE+ ND  
Sbjct: 121 GLTPLESD-VVPCNLGDDRVCVWNMPGLWNDNQDESDESYDSLGKSEEGDYEAEFMNDDY 180

Query: 154 DYALDE--DHDVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLMVQSFRTNDVKESKV 212
           D A DE  + DV+QV   I RDE  V Q  C+GL GQPNDEKLQL+VQS  TNDVKE KV
Sbjct: 181 DDARDEKFEPDVEQVQVEIRRDEVVVLQMGCDGLIGQPNDEKLQLIVQSSGTNDVKEGKV 238

BLAST of Moc05g14880 vs. ExPASy TrEMBL
Match: A0A6J1DTG5 (uncharacterized protein LOC111023843 OS=Momordica charantia OX=3673 GN=LOC111023843 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 2.1e-32
Identity = 83/127 (65.35%), Postives = 95/127 (74.80%), Query Frame = 0

Query: 89  GHDIASLTPLDSDHVVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEY 148
           GHD+  LTPL SD VVPCNL D+RVCDWD     NDNEDES ESY PL  +EEG S+AEY
Sbjct: 8   GHDVEGLTPLGSD-VVPCNLGDDRVCDWDVPGVWNDNEDESGESYDPLAXSEEGHSQAEY 67

Query: 149 GNDANDYALDE--DHDVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLMVQSFRTNDV 208
           GN+ +D ALD+  + DV+QV+  I RDEEAVR   CNGL+G PNDEKLQL+VQS  TNDV
Sbjct: 68  GNEEHDDALDDELEXDVEQVHTEIRRDEEAVRXPGCNGLTGDPNDEKLQLIVQSSGTNDV 127

Query: 209 KESKVFD 211
            E  VFD
Sbjct: 128 NEGDVFD 133

BLAST of Moc05g14880 vs. ExPASy TrEMBL
Match: A0A6J1DQB9 (Reverse transcriptase OS=Momordica charantia OX=3673 GN=LOC111023249 PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 73/114 (64.04%), Postives = 87/114 (76.32%), Query Frame = 0

Query: 102  HVVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEYGNDANDYALDE-- 161
            +VVPCNL D+RVCDWD     NDNEDES ESY PL +++EG S+AEYGN+ +D ALD+  
Sbjct: 1132 NVVPCNLGDDRVCDWDVPGVWNDNEDESGESYDPLAESKEGHSQAEYGNEEHDDALDDEL 1191

Query: 162  DHDVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLMVQSFRTNDVKESKVFD 211
            + DV+QV+  I RDEEAVR   CNGL+G PNDEKLQL+VQS  TNDV E  VFD
Sbjct: 1192 EPDVEQVHTEIRRDEEAVRPPGCNGLTGDPNDEKLQLIVQSSGTNDVNEGDVFD 1245

BLAST of Moc05g14880 vs. ExPASy TrEMBL
Match: A0A6J1DN26 (uncharacterized protein LOC111022090 OS=Momordica charantia OX=3673 GN=LOC111022090 PE=4 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 2.1e-27
Identity = 78/126 (61.90%), Postives = 92/126 (73.02%), Query Frame = 0

Query: 43  NTCHPFLCPQLVSPQFQKPTYRRHSFPSSSSNLSSSEPSHPSYGHIGHDIASLTPLDSDH 102
           ++C P  C     PQFQ+PT    SFPSSSSN SSS+  HP Y H+GHDIA LTPL+SD 
Sbjct: 45  SSCMPTTC----IPQFQRPTDPIPSFPSSSSNPSSSQQPHPYYRHLGHDIAGLTPLESD- 104

Query: 103 VVPCNLRDERVCDWDAS---NDNEDESDESYFPLGKTEEGDSEAEYGNDANDYALDEDH- 162
           VVPCNL D+RVCDW+     NDN+DESDESY PLG++EEGD EAE+ ND  D ALDE+  
Sbjct: 105 VVPCNLGDDRVCDWNVPGLWNDNQDESDESYDPLGESEEGDYEAEFVNDDYDDALDEESE 164

Query: 163 -DVQQV 164
            DV+QV
Sbjct: 165 PDVEQV 165

BLAST of Moc05g14880 vs. ExPASy TrEMBL
Match: A0A6J1DJT1 (uncharacterized protein LOC111020715 OS=Momordica charantia OX=3673 GN=LOC111020715 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.5e-11
Identity = 48/77 (62.34%), Postives = 57/77 (74.03%), Query Frame = 0

Query: 137 EEGDSEAEYGNDANDYALDEDH--DVQQVNNGISRDEEAVRQTVCNGLSGQPNDEKLQLM 196
           EEGD EAE+ ND  D ALDE+   DV+QV+  I RDE AV+Q  C+GL+GQ N E LQL+
Sbjct: 3   EEGDYEAEFVNDDYDDALDEESEPDVEQVHAEICRDEAAVQQMGCDGLTGQHNYENLQLI 62

Query: 197 VQSFRTNDVKESKVFDT 212
           VQS  TNDVKE +VFDT
Sbjct: 63  VQSSGTNDVKEGEVFDT 79

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155970.16.1e-4260.11uncharacterized protein LOC111022954 [Momordica charantia][more]
XP_022157017.14.4e-3265.35uncharacterized protein LOC111023843 [Momordica charantia][more]
XP_022156328.12.5e-2764.04LOW QUALITY PROTEIN: uncharacterized protein LOC111023249 [Momordica charantia][more]
XP_022154947.14.3e-2761.90uncharacterized protein LOC111022090 [Momordica charantia][more]
XP_022153146.17.3e-1162.34uncharacterized protein LOC111020715 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DP003.0e-4260.11uncharacterized protein LOC111022954 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DTG52.1e-3265.35uncharacterized protein LOC111023843 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1DQB91.2e-2764.04Reverse transcriptase OS=Momordica charantia OX=3673 GN=LOC111023249 PE=4 SV=1[more]
A0A6J1DN262.1e-2761.90uncharacterized protein LOC111022090 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DJT13.5e-1162.34uncharacterized protein LOC111020715 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..90
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..142
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 117..146

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g14880.1Moc05g14880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005488 binding